Cmc06g0157391 (gene) Melon (Charmono) v1.1

Overview
NameCmc06g0157391
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein
LocationCMiso1.1chr06: 6271509 .. 6272093 (-)
RNA-Seq ExpressionCmc06g0157391
SyntenyCmc06g0157391
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTATCTCAAATTGCAAGAAAAGTTTGATTCTTTTCCAATTAATTCTCCAATTAACAATAATTCATTCAGCCATAACTCCACAAAGCAGCACAGAGTTCATAAAAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTTCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACCGCCGCTCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCACCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCTCCTCCAGAGATGTCTCTGCTCTTAACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGCAGCAATTTCGAGCTCGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACCGATGAAACCACATGTAGCGAAGGGTTTCAAGGGAAGACGGTGAAGGGAGGCGTTAAGACTGCGGTTAGGAGTAGGATTGTCAACATTGCGCAATTGACGAGTAATGCTCTGTCTTTGATTAATCGAATTGATGATCTGCATTAG

mRNA sequence

ATGGGTATCTCAAATTGCAAGAAAAGTTTGATTCTTTTCCAATTAATTCTCCAATTAACAATAATTCATTCAGCCATAACTCCACAAAGCAGCACAGAGTTCATAAAAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTTCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACCGCCGCTCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCACCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCTCCTCCAGAGATGTCTCTGCTCTTAACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGCAGCAATTTCGAGCTCGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACCGATGAAACCACATGTAGCGAAGGGTTTCAAGGGAAGACGGTGAAGGGAGGCGTTAAGACTGCGGTTAGGAGTAGGATTGTCAACATTGCGCAATTGACGAGTAATGCTCTGTCTTTGATTAATCGAATTGATGATCTGCATTAG

Coding sequence (CDS)

ATGGGTATCTCAAATTGCAAGAAAAGTTTGATTCTTTTCCAATTAATTCTCCAATTAACAATAATTCATTCAGCCATAACTCCACAAAGCAGCACAGAGTTCATAAAAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTTCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACCGCCGCTCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCACCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCTCCTCCAGAGATGTCTCTGCTCTTAACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGCAGCAATTTCGAGCTCGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACCGATGAAACCACATGTAGCGAAGGGTTTCAAGGGAAGACGGTGAAGGGAGGCGTTAAGACTGCGGTTAGGAGTAGGATTGTCAACATTGCGCAATTGACGAGTAATGCTCTGTCTTTGATTAATCGAATTGATGATCTGCATTAG

Protein sequence

MGISNCKKSLILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASISEMPKLRGSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLTSNALSLINRIDDLH
Homology
BLAST of Cmc06g0157391 vs. NCBI nr
Match: XP_011652768.2 (21 kDa protein [Cucumis sativus])

HSP 1 Score: 333.2 bits (853), Expect = 1.5e-87
Identity = 182/194 (93.81%), Postives = 188/194 (96.91%), Query Frame = 0

Query: 1   MGISNCKKSLILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGISN KKSLILFQLILQLT+IHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKST TQILKLSHSHGL SRDVSAL+DCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLT 180
           SEMPKLRG+NF+LAMSNVQTWVSAALTDETTCSEGFQGKTV GGVK AVR++IVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINRIDDLH 195
           SNALSLINRI DLH
Sbjct: 181 SNALSLINRIGDLH 194

BLAST of Cmc06g0157391 vs. NCBI nr
Match: XP_004148138.3 (21 kDa protein [Cucumis sativus])

HSP 1 Score: 332.4 bits (851), Expect = 2.6e-87
Identity = 181/194 (93.30%), Postives = 187/194 (96.39%), Query Frame = 0

Query: 1   MGISNCKKSLILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGISN KK+LILFQLILQLT+IHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKST TQILKLSHSHGL SRDVSALNDCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALNDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLT 180
           SEMPKLRG+NF+LAMSNVQTWVSAALTDETTCSEGFQGKTV GGVK  VR++IVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLT 180

Query: 181 SNALSLINRIDDLH 195
           SNALSLINRI DLH
Sbjct: 181 SNALSLINRIADLH 194

BLAST of Cmc06g0157391 vs. NCBI nr
Match: XP_038882403.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 320.1 bits (819), Expect = 1.3e-83
Identity = 175/194 (90.21%), Postives = 181/194 (93.30%), Query Frame = 0

Query: 1   MGISNCKKSLILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MG+SN KK LILFQLILQLT IHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKS  TQILKLSH+HGL SRDVSALNDCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLT 180
           SEMPKLRG +F+L MSNVQTWVSAALTDETTCSEGFQGK V GGVK  VR+RIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLT 180

Query: 181 SNALSLINRIDDLH 195
           SNALSLINRI DL+
Sbjct: 181 SNALSLINRIADLY 194

BLAST of Cmc06g0157391 vs. NCBI nr
Match: KGN57306.2 (hypothetical protein Csa_011540 [Cucumis sativus])

HSP 1 Score: 312.8 bits (800), Expect = 2.1e-81
Identity = 181/233 (77.68%), Postives = 188/233 (80.69%), Query Frame = 0

Query: 1   MGISNCKKSLILFQLILQLTIIHSAITPQSSTEFIKSSCS-------------------- 60
           MGISN KK+LILFQLILQLT+IHSAITPQSSTEFIKSSCS                    
Sbjct: 60  MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 119

Query: 61  -------------------STTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKST 120
                              STTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKST
Sbjct: 120 TSPRLLATAALSVSLSSVNSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKST 179

Query: 121 TTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASISEMPKLRGSNFELAMSNVQTW 180
            TQILKLSHSHGL SRDVSAL+DCLEELSDSVDSLAASISEMPKLRG+NF+LAMSNVQTW
Sbjct: 180 ATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTW 239

Query: 181 VSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLTSNALSLINRIDDLH 195
           VSAALTDETTCSEGFQGKTV GGVK AVR++IVNIAQLTSNALSLINRI DLH
Sbjct: 240 VSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTSNALSLINRIGDLH 292

BLAST of Cmc06g0157391 vs. NCBI nr
Match: XP_022985514.1 (21 kDa protein-like [Cucurbita maxima])

HSP 1 Score: 306.2 bits (783), Expect = 2.0e-79
Identity = 163/194 (84.02%), Postives = 180/194 (92.78%), Query Frame = 0

Query: 1   MGISNCKKSLILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MG+S CKKSLI  Q+ILQLT+IHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQ
Sbjct: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLAT ALSV+LSSVKS  TQILKLSH+HGL +RDVSALNDCLEELSDSVDSLA+SI
Sbjct: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120

Query: 121 SEMPKLRGSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLT 180
           SEMPKLRG +F+L MS+VQTWVSAALTDETTCSEGFQGK VKGGVK AVR RIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180

Query: 181 SNALSLINRIDDLH 195
           SNALSLINRI +LH
Sbjct: 181 SNALSLINRIAELH 194

BLAST of Cmc06g0157391 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 2.4e-35
Identity = 85/190 (44.74%), Postives = 129/190 (67.89%), Query Frame = 0

Query: 8   KSLILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLA 67
           KS ++F  +L   +I +A +   + +FIK+SC+ TTYP +C  SLS +A  IQ +P+ LA
Sbjct: 5   KSTLVFSALL---VILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELA 64

Query: 68  TAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASISEMPKL- 127
           + AL VSL+  +   T + +L+   GL +R  +A++DCLEE+ DS+D ++ S  EM  L 
Sbjct: 65  STALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLS 124

Query: 128 --RGSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLTSNAL 187
             +G++F   MSNV+TWVSAALTDETTC +GF GK + G +K +VR+++V +A++TSNAL
Sbjct: 125 HAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNAL 184

Query: 188 SLINRIDDLH 195
           +L+N     H
Sbjct: 185 ALVNNFAAKH 191

BLAST of Cmc06g0157391 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 3.5e-34
Identity = 90/198 (45.45%), Postives = 127/198 (64.14%), Query Frame = 0

Query: 5   NCKKSLILFQLILQLTIIHSA--ITPQSS--TEFIKSSCSSTTYPRLCFSSLSVHANAIQ 64
           N + SLILF L L    I  A  +  +SS  TEFIK+SC +T+YP  CF SLS +A+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 65  TSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASI 124
             PR LA  AL+VS++  KS  T + +++   G++ R   A+ DCLEE+ D+VD L+ S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 125 SEMPKLR----GSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNI 184
            E+  L     G +F   +SNV+TW SAALTDET C +GF GK + G +K+ +R+ IV++
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 185 AQLTSNALSLINRIDDLH 195
           A+ TSNAL+LIN     H
Sbjct: 184 AEETSNALALINDFASKH 201

BLAST of Cmc06g0157391 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.4e-32
Identity = 85/189 (44.97%), Postives = 123/189 (65.08%), Query Frame = 0

Query: 12  LFQLILQLTII-------HSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPR 71
           LF  +L   I+        +AIT + +  FI++SC +TTYP +C +SL+ +AN+IQTSPR
Sbjct: 9   LFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPR 68

Query: 72  LLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASISEMP 131
            LA  AL+V+++  +ST   + +L     L  R++ A+ DC+EE+ D+VD L  SI E+ 
Sbjct: 69  RLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEV- 128

Query: 132 KLRGS-----NFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQL 189
           K+ GS      F   MSN QTW SAALT+  TCS+GF G+ + G VK +VR+RI+N+ + 
Sbjct: 129 KMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRG 188

BLAST of Cmc06g0157391 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 5.2e-30
Identity = 76/168 (45.24%), Postives = 116/168 (69.05%), Query Frame = 0

Query: 24  SAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTTT 83
           S  + Q++ ++IK+SC+ T Y  +C++SLS +A+ I+++P+ LA  AL+++LSS KS + 
Sbjct: 137 STYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASK 196

Query: 84  QILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASISEMPKLR---GSNFELAMSNVQT 143
            +  +SH  GL+  +V A+ DC+EE+ DSV SL  SI E+  +     + FE+ MS+V+T
Sbjct: 197 FVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVET 256

Query: 144 WVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLTSNALSLIN 189
           WVSAALT++ TC +GF    VK  VK  VR  +V +A+LTSNAL+LIN
Sbjct: 257 WVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALIN 302

BLAST of Cmc06g0157391 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 4.8e-28
Identity = 82/191 (42.93%), Postives = 121/191 (63.35%), Query Frame = 0

Query: 8   KSLILFQLILQLTIIH-SAITP--QSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ-TSP 67
           K+ I   ++L +TI+  S+ TP    S +FI SSC +T YP LC  +LS +A  I+  + 
Sbjct: 4   KNTIFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNND 63

Query: 68  RLLATAALSVSLSSVKSTTTQILKLS-HSHGLSSRDVSALNDCLEELSDSVDSLAASISE 127
           + LA  AL +SL+  KS T  + KL+  +     R+  A+ DC+E L +SVD LA S+ E
Sbjct: 64  QDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKE 123

Query: 128 MPK----LRGSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQ 187
           + +    +   +F   MSNVQTWVSAALTDETTC +GF  + + G VK  +R ++V++AQ
Sbjct: 124 LARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQ 183

Query: 188 LTSNALSLINR 190
           +TSNAL+L+N+
Sbjct: 184 VTSNALALVNQ 194

BLAST of Cmc06g0157391 vs. ExPASy TrEMBL
Match: A0A0A0LB27 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176245 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 2.1e-87
Identity = 181/194 (93.30%), Postives = 188/194 (96.91%), Query Frame = 0

Query: 1   MGISNCKKSLILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGISN KK+LILFQLILQLT+IHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKST TQILKLSHSHGL SRDVSAL+DCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLT 180
           SEMPKLRG+NF+LAMSNVQTWVSAALTDETTCSEGFQGKTV GGVK AVR++IVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINRIDDLH 195
           SNALSLINRI DLH
Sbjct: 181 SNALSLINRIADLH 194

BLAST of Cmc06g0157391 vs. ExPASy TrEMBL
Match: A0A0A0L8D1 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G175740 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 2.1e-87
Identity = 181/193 (93.78%), Postives = 187/193 (96.89%), Query Frame = 0

Query: 2   GISNCKKSLILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 61
           GISN KKSLILFQLILQLT+IHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT
Sbjct: 44  GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 103

Query: 62  SPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASIS 121
           SPRLLATAALSVSLSSVKST TQILKLSHSHGL SRDVSAL+DCLEELSDSVDSLAASIS
Sbjct: 104 SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 163

Query: 122 EMPKLRGSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLTS 181
           EMPKLRG+NF+LAMSNVQTWVSAALTDETTCSEGFQGKTV GGVK AVR++IVNIAQLTS
Sbjct: 164 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTS 223

Query: 182 NALSLINRIDDLH 195
           NALSLINRI DLH
Sbjct: 224 NALSLINRIGDLH 236

BLAST of Cmc06g0157391 vs. ExPASy TrEMBL
Match: A0A6J1JDH5 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483501 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 9.6e-80
Identity = 163/194 (84.02%), Postives = 180/194 (92.78%), Query Frame = 0

Query: 1   MGISNCKKSLILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MG+S CKKSLI  Q+ILQLT+IHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQ
Sbjct: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLAT ALSV+LSSVKS  TQILKLSH+HGL +RDVSALNDCLEELSDSVDSLA+SI
Sbjct: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120

Query: 121 SEMPKLRGSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLT 180
           SEMPKLRG +F+L MS+VQTWVSAALTDETTCSEGFQGK VKGGVK AVR RIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180

Query: 181 SNALSLINRIDDLH 195
           SNALSLINRI +LH
Sbjct: 181 SNALSLINRIAELH 194

BLAST of Cmc06g0157391 vs. ExPASy TrEMBL
Match: A0A6J1E4J6 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430716 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.8e-79
Identity = 163/194 (84.02%), Postives = 178/194 (91.75%), Query Frame = 0

Query: 1   MGISNCKKSLILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60
           MG+S CKKSLI  Q+ILQLT IHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQ
Sbjct: 1   MGLSICKKSLIFVQIILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASI 120
           TSPRLLAT ALSV+LSSVKS  TQILKLSH+HGL  RDVSALNDCLEELSDSVDSLA+SI
Sbjct: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSI 120

Query: 121 SEMPKLRGSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLT 180
           SEMPKLRG +F+L MS+VQTWVSAALTDETTCSEGFQGK VKGGVK AVR RIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180

Query: 181 SNALSLINRIDDLH 195
           SNALSLINRI +LH
Sbjct: 181 SNALSLINRIAELH 194

BLAST of Cmc06g0157391 vs. ExPASy TrEMBL
Match: A0A6J1JNI8 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111486299 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 6.5e-76
Identity = 161/202 (79.70%), Postives = 179/202 (88.61%), Query Frame = 0

Query: 1   MGISNCKKSLILFQLILQLTI----IHSAITPQ----SSTEFIKSSCSSTTYPRLCFSSL 60
           MG S CK+SLIL   I QLT+    IHSAITPQ    SSTEFIK+SCSSTTYPRLCFSSL
Sbjct: 1   MGFSICKQSLILLVAIFQLTLNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSSL 60

Query: 61  SVHANAIQTSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDS 120
           S+HA +IQTSPRLLATAALSVS+SSVKS TTQILKLSH+HGLS RDVSALNDCLEEL DS
Sbjct: 61  SLHATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDS 120

Query: 121 VDSLAASISEMPKLRGSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSR 180
           +DSL +SISEMPKLRG++F++ MSN+QTWVSAALTDETTCSEGFQGKTV GG+K AVRS+
Sbjct: 121 IDSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSK 180

Query: 181 IVNIAQLTSNALSLINRIDDLH 195
           IVNIAQLTSNA+SLINRI +LH
Sbjct: 181 IVNIAQLTSNAISLINRIAELH 202

BLAST of Cmc06g0157391 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 178.3 bits (451), Expect = 5.8e-45
Identity = 98/185 (52.97%), Postives = 138/185 (74.59%), Query Frame = 0

Query: 10  LILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATA 69
           +I+  L L  T   +  T  ++TEF+KSSC+ TTYPRLCFSSLS HA+ IQTSP+L+A A
Sbjct: 19  IIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHA 78

Query: 70  ALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASISEMPKLRGS 129
           AL+++L+S K T+  +++LS+S  L  ++VSA+ DC+EEL D+++ L  SI EM +L GS
Sbjct: 79  ALNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGS 138

Query: 130 NFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLTSNALSLINR 189
           N+E+ +S++QTWVSAALTD  TC++GF+G+ + G VK  VR RI+ IA LTSNAL+LIN 
Sbjct: 139 NYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALINH 198

Query: 190 IDDLH 195
              +H
Sbjct: 199 FASIH 202

BLAST of Cmc06g0157391 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.0e-37
Identity = 93/186 (50.00%), Postives = 125/186 (67.20%), Query Frame = 0

Query: 11  ILFQLILQLTIIHSAITPQSSTE---FIKSSCSSTTYPRLCFSSLSVHANAIQTSPRLLA 70
           I + L L+LT   +A    +S +   FI+SSC +TTYP LC  SLSV+AN IQTSP+ LA
Sbjct: 12  ISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLA 71

Query: 71  TAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASISEMPKLR 130
             A++V+LS  +ST   + +L+   GL  R+V A+ DC+EE++D+VD L  S+ E+ KL 
Sbjct: 72  ETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQEL-KLC 131

Query: 131 GS-----NFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQLTSN 189
           GS      F   MSN QTW SAALTDE TCS+GF G+ + G +K +VR+RI+N+   TSN
Sbjct: 132 GSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSN 191

BLAST of Cmc06g0157391 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 146.4 bits (368), Expect = 2.5e-35
Identity = 90/198 (45.45%), Postives = 127/198 (64.14%), Query Frame = 0

Query: 5   NCKKSLILFQLILQLTIIHSA--ITPQSS--TEFIKSSCSSTTYPRLCFSSLSVHANAIQ 64
           N + SLILF L L    I  A  +  +SS  TEFIK+SC +T+YP  CF SLS +A+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 65  TSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASI 124
             PR LA  AL+VS++  KS  T + +++   G++ R   A+ DCLEE+ D+VD L+ S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 125 SEMPKLR----GSNFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNI 184
            E+  L     G +F   +SNV+TW SAALTDET C +GF GK + G +K+ +R+ IV++
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 185 AQLTSNALSLINRIDDLH 195
           A+ TSNAL+LIN     H
Sbjct: 184 AEETSNALALINDFASKH 201

BLAST of Cmc06g0157391 vs. TAIR 10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 141.0 bits (354), Expect = 1.0e-33
Identity = 85/189 (44.97%), Postives = 123/189 (65.08%), Query Frame = 0

Query: 12  LFQLILQLTII-------HSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSPR 71
           LF  +L   I+        +AIT + +  FI++SC +TTYP +C +SL+ +AN+IQTSPR
Sbjct: 9   LFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPR 68

Query: 72  LLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASISEMP 131
            LA  AL+V+++  +ST   + +L     L  R++ A+ DC+EE+ D+VD L  SI E+ 
Sbjct: 69  RLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEV- 128

Query: 132 KLRGS-----NFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQL 189
           K+ GS      F   MSN QTW SAALT+  TCS+GF G+ + G VK +VR+RI+N+ + 
Sbjct: 129 KMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRG 188

BLAST of Cmc06g0157391 vs. TAIR 10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 132.9 bits (333), Expect = 2.8e-31
Identity = 84/196 (42.86%), Postives = 120/196 (61.22%), Query Frame = 0

Query: 6   CKKSLILFQLILQLTII--HSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQTSP 65
           C   L+   L+ Q TI    S+  P SS  FI SSC  T Y  LC   L+  A+ I+ + 
Sbjct: 9   CYCLLLFLPLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNE 68

Query: 66  RLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASISEM 125
             LA  AL+V+L  V+STT  + KL+ +  +  R+  A+ DC+E L D ++ LA S+ E+
Sbjct: 69  NQLAQTALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMREL 128

Query: 126 PKLRGS-----NFELAMSNVQTWVSAALTDETTCSEGFQGKTVKGGVKTAVRSRIVNIAQ 185
            ++  S      F   +SNV+TWVSAALTDETTC +GF GK + G VK+A+R R+V++A+
Sbjct: 129 KQVGRSGRDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVAR 188

Query: 186 LTSNALSLINRIDDLH 195
           +TSNAL+L+NR    H
Sbjct: 189 VTSNALALVNRFAARH 204

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011652768.21.5e-8793.8121 kDa protein [Cucumis sativus][more]
XP_004148138.32.6e-8793.3021 kDa protein [Cucumis sativus][more]
XP_038882403.11.3e-8390.2121 kDa protein-like [Benincasa hispida][more]
KGN57306.22.1e-8177.68hypothetical protein Csa_011540 [Cucumis sativus][more]
XP_022985514.12.0e-7984.0221 kDa protein-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P174072.4e-3544.7421 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1[more]
Q9SB373.5e-3445.45Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1[more]
Q9STY51.4e-3244.97Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1[more]
Q9SI745.2e-3045.24Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1[more]
Q9SI724.8e-2842.93Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LB272.1e-8793.30PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176245 PE=4 S... [more]
A0A0A0L8D12.1e-8793.78PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G175740 PE=4 S... [more]
A0A6J1JDH59.6e-8084.0221 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483501 PE=4 SV=1[more]
A0A6J1E4J62.8e-7984.0221 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430716 PE=4 SV=1[more]
A0A6J1JNI86.5e-7679.7021 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111486299 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G62360.15.8e-4552.97Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G62350.12.0e-3750.00Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT4G25260.12.5e-3545.45Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT3G47380.11.0e-3344.97Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT4G12390.12.8e-3142.86pectin methylesterase inhibitor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 30..186
e-value: 6.0E-49
score: 178.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 34..186
e-value: 6.9E-37
score: 127.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 7..189
e-value: 8.6E-37
score: 124.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 29..191
e-value: 8.7E-40
score: 138.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 33..190
NoneNo IPR availablePANTHERPTHR31080PECTINESTERASE INHIBITOR-LIKEcoord: 22..190
NoneNo IPR availablePANTHERPTHR31080:SF16121 KDA PROTEIN-LIKEcoord: 22..190
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 37..191
e-value: 1.00503E-40
score: 133.718

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc06g0157391.1Cmc06g0157391.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0009827 plant-type cell wall modification
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004222 metalloendopeptidase activity