Cmc05g0146031 (gene) Melon (Charmono) v1.1

Overview
NameCmc05g0146031
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionVacuolar protein sorting-associated protein 32-like protein 2
LocationCMiso1.1chr05: 28789571 .. 28794287 (+)
RNA-Seq ExpressionCmc05g0146031
SyntenyCmc05g0146031
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCACTATTGAATTGATTATATTTTGCTAGGAACATATTTGAGAGTTGGTTCATAGTGGGCTTGTAGGTATGCTAACTAGTTCGGCCCATATACATGTCAGGAGCCCAACTGAGAGTTTGGGCCGACTCATAGATAAGACAATCGGCCTATATTAAAATATCTATTGAGCTCGGCCCAAATACTCATTTAAATAGAATTGGCGGAGAGGAAAATGGGAGTTTTGATTTGATGAACAGAAACCAATAAGTAGATTGCCGCGTGGAGACGAAACAGCGACTTGAAGAGAAAGGGAACTCCAAATCTTCGACCTCCGTTTTCCGGAGCGTCTGACGAACCCTTTCTACAATTCTACTTCAAATTTTTTCATTGAATCTTTCGATTCATTCTCTGTTTTCTGAATTCGTTTCGGGTTCTCATCTTTCTGTTTTCAATCTCTCTGGTTTCTCGACTTTTGGTACACATTGATTACTTTTTCCTTTTCTTTTTCATTCATGTTGAATCCAAGCGTTTGAATCTGATTTTGTTCGTTGTTGTAGAAGATCGAGAGAGGATTCTTGTTGCTTTGTATCAACAATGTTTAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTGGAGAAGCTAAGCGAGGTGCGGCCTCATTTTGTGATTTTATTTTTTTAGATTCATATTCTTTTTGTGGAAACGATATTTTCTTGTGTGTCGTTGGTTTTAAGCTCTAGTTCTATTTCAACAATTTTGATGTACGCTTGACTTCTCGATCATTCATTTTTTCCAAATGGTAGTGTTTAGGACGTCGGTATCATTGGTTTTCACGTCCCCTTGACCGTGTATTAGTGTTTATTTGGTTTTTACTTGGTCAAGTGGCGGTTTCTGGTTGAAAATTTTTCCGTATTTGAATTGGATTAACTGTTATTATTATTATTTTTTTGTTATGCGAATGGTAATCGGTGTGTTTTAGGTGAAATTACACTTATGTAAGTTAATTGGTTGTTATTCTTCTATGCTGCTATATTGAAAGAGTAGATAACTGGAATCATGTCTGAGACGATTGTAATTGTGATTGTATAGACTTTTGTAGTCTTGCAAAAATTTTCGTGATTGATACCAATGTTTCTGGGTACTCCTTTTTATTGTCTGTAATTCTCTGTTTAGACACTTGAAATGCTGGAGAAGAAAGAGAATGTTCTTGTGAAGAAGGCAGCTGCAGAGGTTGAAAGAGCTAAAGAATTCACAAGAGTGAAGAATAAACGAGGTATGGTCTGAAATTGTGAGTGCAGTAGCAGTATGAATTTTTGTTTCCTATGTTAGCATTTCTTACATGTGTGACGTGTGAATAACATTTCATTAATACGTATATTGTCCGCTCTTAGGTGTAAACTTGTTGGTAGCACCATACCTGTTTTCTCATGATTTGGAATTGCAATTTATGCTTCTGTCAATCCTTTGCAATGTGATAATCTGCAGAAACTTTTTAATGCTGTCTTTCGTGTACATTTTTGTTCATTATTATTAGGAGAATCTTGTGGTCAATGAATCCTTCGACTCTGTATCCTCAATGAAATTAATTTTGATTTTTCAAAAATTGTCCATGTTAATCTCAAGATTCTGTAGAGTCAAGGTTTTACTGTTATATTAGTAAAATTACTAGACAGGCTTGCATGGTTGGTCGTTCTTACCTTGTGCTCTTCTGGCTTTTGCTACTGTCTTTATGGCTTGCAGTTGAAATGTAACCTTTCTATTTCTTTTATAATAGGGTTGTGTATGGCCAAGATCTGTAGACTTGATTACTTCAATGCTTCGTTTTCGATTTCTGTCCTTTTTAGATTTGCCTTGTTCTTATCATTATTAGTAGCTTTGGATAATTGTTTTCAAATAGTTTAATTTCCTAGTTATTTGTCTCTTTTGTTGAGAACTGGAGCTTTGGAAATTGTTCTGTTTCCTTTTTTTAATCTATATCTTTCCTTTACATTTTTCTTTTTTATTTATTTATATCAAAATCATTGTTACCTTTGTTCTTGCTTATCCTCAACTCCCTATTTTACCTTCTGTCCATATCCCAGAGGCCTTGGATATTGAATAATTTTGTTTAGGATTTAAATATTTTTCCTACACAAAAATTAACATTTGGAAATTAATCAACTATCGTAAATCTTACCGGTTGTATATAAGAATCAAATTTATGTAATCAATTAATTAGATTGAACCTAATAGATTACTGATACTGAATTATTTAAAATATCCTTCACAGATCAAATCTTACGTAATTCAGTACATTACTATGTTTTATTTTGCTTCTGTTTTTAAGAGTCCATTCATAGTCATTGTAATTTGTTTCCAGCGGCTATACAATGTTTGAAGAGAAAGAGGCTATATGAACAGCAAATAGAGCAGCTTGGAAATTTCCAATTGCGCATCCATGACCAAGTAAGTTGTAGTTGTTACTTACAGATTGATGAGACATCAATTTCTCTTTTTTACTTCCTTTAACTACAATTCATGGAAGTGTTAACTTTATTCAAAACATTTCTTACTCTTGCAGATGATTATGTTAGAAGGGGCAAAAGCTACAACGGAGACTGTAGATGCTCTAAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACGTATGCCTGCTTTACTTGCGCTCATTTTGCCTTTGTGGTTTATAACTTTTCAGAATTTTGGTCATTGAATATTCCTAGTTTTATTGTTTATTTTTTTTACATGGCCGGATTATTAAAAAAAAAAAAAAAAAAAACTTTACAACATATCTGCACTTATTTAGTAACTTTATTCATCTAGTTATTGATATTAAGCTAACAGTCTGAGAAAACAGCCTTCGAATGCCAGCAGATTTTGAAAAATGTTTGTGAGATTTTGTTTCAAGTCTTATCGTGAAGTGTTAGACATGCACATCTTATGTGGAAAGTAGGCTGATTATTTGGAGCTTTCTTGTTCAGGAATATTGATGATGTGGACAAGACAATGGATGAGATCAACGAGCAGACTGAGAATATGAAGCAGATCCAGGAAGCGTTATCAGCTCCAATTGGTGCTGCAGCTGATTTCGATGAGGTTGTGTTAATCTTCTAATAGTATCTTGAGGTTTTCTTGTGATTATAGGCTTTATATTAACAAGGGTGTGGTACTCAGGATGAATTGGAGGCAGAACTTGAAGATCTTGAAAGTGCGGAGTTGGAAGAGCAACTTCTCCAACCAGCATCAACAGCTCCAGCTGCACCTGTTTCAGTCCCGGCAGGCCGAGTTCCTGCACGCCCTGCTCCTCAAAAGAGAACTGCCGAAGAAGACGAACTCGCTGCTTTGCAAGCTGAGATGGCACTCTAATCAGGTTTTTTCTATTTTATTTGAGTTCAACACGAAAAAGAAAAGGGAGTATATCCAAGCAAGTTTGTTGTTGTAACATTGTAACTGAGTTTTGATTGGAAATTGCAGGTAAATGGGATGGATCATACATACATTCATTCATATATGTCGTGGCTGACTGATTGTGTGCATCATCATCATCATCCAAAGAAATCTTCTGACGAGTGTTTTTATACGAATTATTAATATTCCCTGACGCAAGACGGTGTATATAAAGTCTGCTAAATGATTGGAATTGTGATTTACATCTTTTTATTTATTTATTTTCTATTTTGCAGAGTTTGGGAACGCCTTGTCTATTTTTTTTTTCCGAAAAACTTGCATTCACGTTCACCCCCTCGGCAAGTGTAGTAGGTAAAGTAAGGTATACTCGAGTTTGGTCATTACATAAAAAGACAAACTCTAATCGGTTTATGCGCAAGTCAATTTATCATAATTTCTTAAATCGAGGACAAATTTTTTTATTAAAATGTACTTAACTATTGCATGAATTTTACTTCACTTCAAGGGATTGAAATTTGAGTATCTAAACAAAGTAGCTGACAAGTTAGGGCTCCCGCATATATTTTTAATCAAGTATTGTAAATGAATGATTGTTTTCATACAACTTATTCATGGAATTTCATTTTCTAAATGTTTGCTTTGACCTTTGTTTTCTCATTTTTTTAATTCCTTGATAAATTTTAAAAATAGAAATGGACCTTTGAAAACTTAACTTACTATAGTTGAAAATGTCAATAAAAAAGTTTTGTAAATCCGCATTTATTTTGTAGAATGTTGAAAATTTATCATGATCCTCGGTTTTTATTTTTTCGATAGGTGTGGAATCAAGGTTTACGACAAGGAAAAATGAGCCAAGAGCTGCTTTGTTCACATCGATGATAGACTATTGTTATACAAAACATTTGGAAAATGATCAGTGTTATAGACTACACAAACTAATTACATTCAAAAAACGATCAACGTTAAAGCCTACAAAAACTAATCCAATTTTGGCCAGTTCTAAAAAGTACTGAAATTGATATCTAAAAGGAGAGATTCTGAAGTTCCTTTTCCTAAAAAATTAGATGAAATCCCATATTCAGTATTTATTCTGGCGTTGAAATTATTCCAACATCGTTTTCTTTTTTTGAAAAGGTTTATGGAGTAAGATAAATGTGCCAAGGGTCATAATTGATGCTTCAATGGTTTTGATTAAAGCAGCACCCAAAAACCTTATTTAAACAGCCAATGCTAGCTTAAAAAAATAGCCATATTTGGCAAGTTCCAAACAGACCTGGAAAACAAGCCCCGGTGTCCAAATTAGTC

mRNA sequence

TCACTATTGAATTGATTATATTTTGCTAGGAACATATTTGAGAGTTGGTTCATAGTGGGCTTGTAGGTATGCTAACTAGTTCGGCCCATATACATGTCAGGAGCCCAACTGAGAGTTTGGGCCGACTCATAGATAAGACAATCGGCCTATATTAAAATATCTATTGAGCTCGGCCCAAATACTCATTTAAATAGAATTGGCGGAGAGGAAAATGGGAGTTTTGATTTGATGAACAGAAACCAATAAGTAGATTGCCGCGTGGAGACGAAACAGCGACTTGAAGAGAAAGGGAACTCCAAATCTTCGACCTCCGTTTTCCGGAGCGTCTGACGAACCCTTTCTACAATTCTACTTCAAATTTTTTCATTGAATCTTTCGATTCATTCTCTGTTTTCTGAATTCGTTTCGGGTTCTCATCTTTCTGTTTTCAATCTCTCTGGTTTCTCGACTTTTGATCGAGAGAGGATTCTTGTTGCTTTGTATCAACAATGTTTAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTGGAGAAGCTAAGCGAGACACTTGAAATGCTGGAGAAGAAAGAGAATGTTCTTGTGAAGAAGGCAGCTGCAGAGGTTGAAAGAGCTAAAGAATTCACAAGAGTGAAGAATAAACGAGCGGCTATACAATGTTTGAAGAGAAAGAGGCTATATGAACAGCAAATAGAGCAGCTTGGAAATTTCCAATTGCGCATCCATGACCAAATGATTATGTTAGAAGGGGCAAAAGCTACAACGGAGACTGTAGATGCTCTAAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACGAATATTGATGATGTGGACAAGACAATGGATGAGATCAACGAGCAGACTGAGAATATGAAGCAGATCCAGGAAGCGTTATCAGCTCCAATTGGTGCTGCAGCTGATTTCGATGAGGATGAATTGGAGGCAGAACTTGAAGATCTTGAAAGTGCGGAGTTGGAAGAGCAACTTCTCCAACCAGCATCAACAGCTCCAGCTGCACCTGTTTCAGTCCCGGCAGGCCGAGTTCCTGCACGCCCTGCTCCTCAAAAGAGAACTGCCGAAGAAGACGAACTCGCTGCTTTGCAAGCTGAGATGGCACTCTAATCAGGTAAATGGGATGGATCATACATACATTCATTCATATATGTCGTGGCTGACTGATTGTGTGCATCATCATCATCATCCAAAGAAATCTTCTGACGAGTGTTTTTATACGAATTATTAATATTCCCTGACGCAAGACGGTGTATATAAAGTCTGCTAAATGATTGGAATTGTGATTTACATCTTTTTATTTATTTATTTTCTATTTTGCAGAGTTTGGGAACGCCTTGTCTATTTTTTTTTTCCGAAAAACTTGCATTCACGTTCACCCCCTCGGCAAGTGTAGTAGGTAAAGTAAGGTGTGGAATCAAGGTTTACGACAAGGAAAAATGAGCCAAGAGCTGCTTTGTTCACATCGATGATAGACTATTGTTATACAAAACATTTGGAAAATGATCAGTGTTATAGACTACACAAACTAATTACATTCAAAAAACGATCAACGTTAAAGCCTACAAAAACTAATCCAATTTTGGCCAGTTCTAAAAAGTACTGAAATTGATATCTAAAAGGAGAGATTCTGAAGTTCCTTTTCCTAAAAAATTAGATGAAATCCCATATTCAGTATTTATTCTGGCGTTGAAATTATTCCAACATCGTTTTCTTTTTTTGAAAAGGTTTATGGAGTAAGATAAATGTGCCAAGGGTCATAATTGATGCTTCAATGGTTTTGATTAAAGCAGCACCCAAAAACCTTATTTAAACAGCCAATGCTAGCTTAAAAAAATAGCCATATTTGGCAAGTTCCAAACAGACCTGGAAAACAAGCCCCGGTGTCCAAATTAGTC

Coding sequence (CDS)

ATGTTTAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTGGAGAAGCTAAGCGAGACACTTGAAATGCTGGAGAAGAAAGAGAATGTTCTTGTGAAGAAGGCAGCTGCAGAGGTTGAAAGAGCTAAAGAATTCACAAGAGTGAAGAATAAACGAGCGGCTATACAATGTTTGAAGAGAAAGAGGCTATATGAACAGCAAATAGAGCAGCTTGGAAATTTCCAATTGCGCATCCATGACCAAATGATTATGTTAGAAGGGGCAAAAGCTACAACGGAGACTGTAGATGCTCTAAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACGAATATTGATGATGTGGACAAGACAATGGATGAGATCAACGAGCAGACTGAGAATATGAAGCAGATCCAGGAAGCGTTATCAGCTCCAATTGGTGCTGCAGCTGATTTCGATGAGGATGAATTGGAGGCAGAACTTGAAGATCTTGAAAGTGCGGAGTTGGAAGAGCAACTTCTCCAACCAGCATCAACAGCTCCAGCTGCACCTGTTTCAGTCCCGGCAGGCCGAGTTCCTGCACGCCCTGCTCCTCAAAAGAGAACTGCCGAAGAAGACGAACTCGCTGCTTTGCAAGCTGAGATGGCACTCTAA

Protein sequence

MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPASTAPAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL
Homology
BLAST of Cmc05g0146031 vs. NCBI nr
Match: XP_008459153.1 (PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_008459161.1 PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_008459166.1 PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_038889616.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Benincasa hispida] >XP_038889617.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Benincasa hispida] >KAA0061506.1 vacuolar protein sorting-associated protein 32-like protein 2 [Cucumis melo var. makuwa] >TYK10767.1 vacuolar protein sorting-associated protein 32-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 390.6 bits (1002), Expect = 9.0e-105
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of Cmc05g0146031 vs. NCBI nr
Match: XP_004141038.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_011648616.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_031736822.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >KGN60554.1 hypothetical protein Csa_019460 [Cucumis sativus])

HSP 1 Score: 386.3 bits (991), Expect = 1.7e-103
Identity = 216/218 (99.08%), Postives = 216/218 (99.08%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTR KNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVP GRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of Cmc05g0146031 vs. NCBI nr
Match: KAG7030473.1 (Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 380.2 bits (975), Expect = 1.2e-101
Identity = 209/218 (95.87%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKA+AEVE+AKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 219
            PAAPVS+P+GRVPARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of Cmc05g0146031 vs. NCBI nr
Match: XP_023545615.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545616.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545619.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545620.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547008.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547009.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547010.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547011.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 378.6 bits (971), Expect = 3.5e-101
Identity = 209/218 (95.87%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKA+AEVE+AKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 219
            PAAPVSVP+GRVPARP PQKR AEEDELAALQAEMAL
Sbjct: 181 TPAAPVSVPSGRVPARPVPQKRNAEEDELAALQAEMAL 218

BLAST of Cmc05g0146031 vs. NCBI nr
Match: XP_022946105.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022946106.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022946107.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022946108.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata])

HSP 1 Score: 377.5 bits (968), Expect = 7.9e-101
Identity = 207/218 (94.95%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKA+AEVE+AKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMD+INEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDDINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 219
            PAAPVS+P+GRVPARP PQKRTAEEDEL ALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELTALQAEMAL 218

BLAST of Cmc05g0146031 vs. ExPASy Swiss-Prot
Match: Q9SZE4 (Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS32.2 PE=1 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 3.1e-92
Identity = 189/219 (86.30%), Postives = 203/219 (92.69%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANAL TL+KL+ETLEMLEKKE VL+KKA AEVE+AKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE Q+EQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+ P+GAAADFDEDEL AEL++LES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAAP-VSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 219
           AP  P V VPAGR PARP PQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of Cmc05g0146031 vs. ExPASy Swiss-Prot
Match: O82197 (Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS32.1 PE=1 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 1.1e-84
Identity = 181/217 (83.41%), Postives = 193/217 (88.94%), Query Frame = 0

Query: 3   NRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAIQC 62
           NRLFGKPKQE + L TL+KL+ETLEMLEKKENVL+KKA  EVE+AKEF+R KNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 63  LKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 122
           LKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 123 DKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPASTAP 182
           DKTMDEINEQTENMKQIQEALSAP G A DFDEDELEAEL++LE AELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 183 AAPVSVPAGRVPAR-PAPQKRTAEEDELAALQAEMAL 219
             P+ VP G  PAR PA ++ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of Cmc05g0146031 vs. ExPASy Swiss-Prot
Match: Q7ZVC4 (Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.1e-27
Identity = 88/217 (40.55%), Postives = 128/217 (58.99%), Query Frame = 0

Query: 1   MFNRLF---GKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKR 60
           +F ++F   GK  + A+    +++L ET EML KK+  L KK   E+  AK+    KNKR
Sbjct: 3   LFGKMFGSGGKGGKSASPQEAIQRLRETEEMLTKKQEFLEKKIEQELVTAKK-NGTKNKR 62

Query: 61  AAIQCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENAHTNTEVIKNMGYAAKAMKAAHDNM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQP 180
           +ID VD+ M +I EQ E  ++I +A+S P+G   +FDEDEL AELE+LE  EL++ LL+ 
Sbjct: 123 DIDKVDELMQDIIEQQELAQEISDAISKPVGFGEEFDEDELLAELEELEQEELDKNLLEI 182

Query: 181 ASTAPAAPVSVPAGRVPARPAPQKRTAEEDELAALQA 215
               P    +VP+  +P+RPA +K   +ED++  L+A
Sbjct: 183 GDNVPLP--NVPSTSLPSRPAKKKEEEDEDDMKDLEA 216

BLAST of Cmc05g0146031 vs. ExPASy Swiss-Prot
Match: Q6IQ73 (Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 8.7e-26
Identity = 91/217 (41.94%), Postives = 122/217 (56.22%), Query Frame = 0

Query: 1   MFNRLF---GKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKR 60
           +F +LF   GK  +       ++KL ET EML KK++ L KK  AE+  AK+    KNKR
Sbjct: 3   VFGKLFGGGGKGGKGPTPQEAIQKLRETEEMLAKKQDFLEKKIDAELLIAKK-NGTKNKR 62

Query: 61  AAIQCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMK   +  
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKTAHENM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQP 180
           +ID VD  M +I EQ E  ++I +A+S P+G   +FDEDEL AELE+LE  EL++ LLQ 
Sbjct: 123 DIDKVDDLMQDITEQQELAQEISDAISRPVGFGEEFDEDELMAELEELEQEELDKDLLQ- 182

Query: 181 ASTAPAAPVSVPAGRVPARPAP------QKRTAEEDE 209
                + P  VP   VP+ P P      QK+  EEDE
Sbjct: 183 ----ISGPEDVPLPNVPSNPLPKKTAVAQKKRQEEDE 213

BLAST of Cmc05g0146031 vs. ExPASy Swiss-Prot
Match: Q5XGW6 (Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.5e-25
Identity = 81/207 (39.13%), Postives = 120/207 (57.97%), Query Frame = 0

Query: 7   GKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAIQCLKRK 66
           GK  +  +    ++KL +T EML KK+  L KK   E+  AK+    KNKRAA+Q LKRK
Sbjct: 12  GKGAKGPSPQEAIQKLRDTEEMLAKKQEFLEKKIEQELVTAKKH-GTKNKRAALQALKRK 71

Query: 67  RLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTM 126
           + YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     +I+ VD+ M
Sbjct: 72  KRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKAAHDNMDIEKVDELM 131

Query: 127 DEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPASTAPAAPV 186
            +I +Q E  ++I +A+S P+G   DFDEDEL AELE+LE  EL++ LL+          
Sbjct: 132 QDIADQQELAQEISDAISKPVGFGEDFDEDELMAELEELEQEELDKNLLEVQGPETVPLP 191

Query: 187 SVPAGRVPARPAPQKRTAEEDELAALQ 214
           +VPA  +PA+P  +K+  ++D++  L+
Sbjct: 192 NVPAASLPAKPVKKKQEEDDDDMRELE 217

BLAST of Cmc05g0146031 vs. ExPASy TrEMBL
Match: A0A1S3CAQ7 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656 GN=LOC103498351 PE=4 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.4e-105
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of Cmc05g0146031 vs. ExPASy TrEMBL
Match: A0A5A7V4V7 (Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00800 PE=4 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.4e-105
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of Cmc05g0146031 vs. ExPASy TrEMBL
Match: A0A0A0LHT5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000620 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 8.2e-104
Identity = 216/218 (99.08%), Postives = 216/218 (99.08%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTR KNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVP GRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of Cmc05g0146031 vs. ExPASy TrEMBL
Match: A0A6J1G2S3 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111450291 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 3.8e-101
Identity = 207/218 (94.95%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKA+AEVE+AKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMD+INEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDDINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 219
            PAAPVS+P+GRVPARP PQKRTAEEDEL ALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELTALQAEMAL 218

BLAST of Cmc05g0146031 vs. ExPASy TrEMBL
Match: A0A6J1FBK1 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata OX=3662 GN=LOC111444128 PE=4 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 6.5e-101
Identity = 207/218 (94.95%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKAAAEVE+AKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APA PVSVP+GRVPARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of Cmc05g0146031 vs. TAIR 10
Match: AT4G29160.1 (SNF7 family protein )

HSP 1 Score: 339.3 bits (869), Expect = 2.2e-93
Identity = 189/219 (86.30%), Postives = 203/219 (92.69%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANAL TL+KL+ETLEMLEKKE VL+KKA AEVE+AKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE Q+EQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+ P+GAAADFDEDEL AEL++LES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAAP-VSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 219
           AP  P V VPAGR PARP PQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of Cmc05g0146031 vs. TAIR 10
Match: AT4G29160.3 (SNF7 family protein )

HSP 1 Score: 339.3 bits (869), Expect = 2.2e-93
Identity = 189/219 (86.30%), Postives = 203/219 (92.69%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANAL TL+KL+ETLEMLEKKE VL+KKA AEVE+AKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE Q+EQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+ P+GAAADFDEDEL AEL++LES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAAP-VSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 219
           AP  P V VPAGR PARP PQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of Cmc05g0146031 vs. TAIR 10
Match: AT2G19830.1 (SNF7 family protein )

HSP 1 Score: 314.3 bits (804), Expect = 7.6e-86
Identity = 181/217 (83.41%), Postives = 193/217 (88.94%), Query Frame = 0

Query: 3   NRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAIQC 62
           NRLFGKPKQE + L TL+KL+ETLEMLEKKENVL+KKA  EVE+AKEF+R KNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 63  LKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 122
           LKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 123 DKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPASTAP 182
           DKTMDEINEQTENMKQIQEALSAP G A DFDEDELEAEL++LE AELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 183 AAPVSVPAGRVPAR-PAPQKRTAEEDELAALQAEMAL 219
             P+ VP G  PAR PA ++ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of Cmc05g0146031 vs. TAIR 10
Match: AT4G29160.2 (SNF7 family protein )

HSP 1 Score: 295.4 bits (755), Expect = 3.7e-80
Identity = 166/192 (86.46%), Postives = 178/192 (92.71%), Query Frame = 0

Query: 28  MLEKKENVLVKKAAAEVERAKEFTRVKNKRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQM 87
           MLEKKE VL+KKA AEVE+AKE++R KNKRAAIQCLKRKRLYE Q+EQLGNFQLRIHDQM
Sbjct: 1   MLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAIQCLKRKRLYEGQVEQLGNFQLRIHDQM 60

Query: 88  IMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSAPI 147
           IMLEGAKATTETVDALR+GASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEAL+ P+
Sbjct: 61  IMLEGAKATTETVDALRSGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALATPM 120

Query: 148 GAAADFDEDELEAELEDLESAELEEQLLQPASTAPAAP-VSVPAGRVPARPAPQKRTAEE 207
           GAAADFDEDEL AEL++LES ELE QLLQPA+TAP  P V VPAGR PARP PQKRTAEE
Sbjct: 121 GAAADFDEDELAAELDELESEELESQLLQPATTAPPLPSVPVPAGRQPARPVPQKRTAEE 180

Query: 208 DELAALQAEMAL 219
           +ELAALQAEMAL
Sbjct: 181 EELAALQAEMAL 192

BLAST of Cmc05g0146031 vs. TAIR 10
Match: AT5G09260.1 (vacuolar protein sorting-associated protein 20.2 )

HSP 1 Score: 58.2 bits (139), Expect = 9.9e-09
Identity = 60/199 (30.15%), Postives = 96/199 (48.24%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60
           M N    KPK      A L  L      L + +  L K   AE + A++  R K K  A+
Sbjct: 1   MGNLFVKKPKITEVDRAIL-SLKTQRRKLGQYQQQLEKVIEAEKQAARDLIREKRKDRAL 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
             LK+KR  E+ ++Q+  + + +  Q+  +E         ++L+ G +A+KA+Q   N+D
Sbjct: 61  LALKKKRTQEELLKQVDQWLINVEQQLADIELTSKQKAVFESLKQGNNAIKAIQSEVNLD 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADF-DEDELEAELEDLESAELEEQLLQPAS 180
           DV K MD+    T   K  Q+ LSA +G      DE+E+ AE ++LES  + E + +  +
Sbjct: 121 DVQKLMDD----TAEAKAYQDELSAILGEKLSAEDEEEILAEFDNLESLLIVEDMPEVPT 180

Query: 181 T--APAAPVSVPAGRVPAR 197
           T   P  P  +    VP +
Sbjct: 181 TELMPEEPEKMDLPDVPTK 194

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008459153.19.0e-105100.00PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis mel... [more]
XP_004141038.11.7e-10399.08vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_0... [more]
KAG7030473.11.2e-10195.87Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma su... [more]
XP_023545615.13.5e-10195.87vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo su... [more]
XP_022946105.17.9e-10194.95vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschat... [more]
Match NameE-valueIdentityDescription
Q9SZE43.1e-9286.30Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana... [more]
O821971.1e-8483.41Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana... [more]
Q7ZVC42.1e-2740.55Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=... [more]
Q6IQ738.7e-2641.94Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=... [more]
Q5XGW62.5e-2539.13Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A1S3CAQ74.4e-105100.00vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656... [more]
A0A5A7V4V74.4e-105100.00Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo va... [more]
A0A0A0LHT58.2e-10499.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000620 PE=4 SV=1[more]
A0A6J1G2S33.8e-10194.95vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita mosch... [more]
A0A6J1FBK16.5e-10194.95vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata O... [more]
Match NameE-valueIdentityDescription
AT4G29160.12.2e-9386.30SNF7 family protein [more]
AT4G29160.32.2e-9386.30SNF7 family protein [more]
AT2G19830.17.6e-8683.41SNF7 family protein [more]
AT4G29160.23.7e-8086.46SNF7 family protein [more]
AT5G09260.19.9e-0930.15vacuolar protein sorting-associated protein 20.2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 12..39
NoneNo IPR availableCOILSCoilCoilcoord: 122..142
NoneNo IPR availableCOILSCoilCoilcoord: 151..175
NoneNo IPR availableGENE3D1.10.287.1060coord: 14..91
e-value: 3.4E-22
score: 80.3
NoneNo IPR availableGENE3D6.10.250.1710coord: 117..172
e-value: 2.4E-8
score: 35.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 176..207
NoneNo IPR availablePANTHERPTHR22761CHARGED MULTIVESICULAR BODY PROTEINcoord: 1..218
NoneNo IPR availablePANTHERPTHR22761:SF63VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 32 HOMOLOG 1coord: 1..218
IPR005024Snf7 familyPFAMPF03357Snf7coord: 20..185
e-value: 8.0E-46
score: 155.8

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc05g0146031.1Cmc05g0146031.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070676 intralumenal vesicle formation
biological_process GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway
biological_process GO:0007034 vacuolar transport
cellular_component GO:0009898 cytoplasmic side of plasma membrane
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body