Cmc05g0145221 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc05g0145221
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	tRNA (guanine(37)-N1)-methyltransferase
Location	CMiso1.1chr05: 28234483 .. 28239260 (-)
RNA-Seq Expression	Cmc05g0145221
Synteny	Cmc05g0145221

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTAACTAATTCCCAGTTCGAAATACTGTCTAAACGACGTCGTGTAGTGGTCTCCTAATTTTAGCTTAAACCCTAGTCCTGTCGCCGCCGGTGGTCACAGCAGTCTCCGGCGACAGTTCCCGCTGTTAGTCTTCTACTACGGTGAAAATCTCGATTCTGATTCTTTCTATCCGGTGGAAAAATCTCCGAAACCATTACTCTACATTGTTCTATTCTGATTTGAATTTGTTTGTATTGAATTCGGTGTAATTTTTGTTGTGTTATTGAGTATTGAGAAAAATCGAACTTGTGAATCGATCAATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCATTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACGCCATGTTTTGTGTTATATTGTTTCTTTGTTATACTTTGCCAATTATTCGATTTCAATTAATCATCTGGGTAATGGCATATAGTATTAGTGTTACTTATATTTTGTTCTCGTTGAACTGTTCATCTTTCTCATCCTAGTTCATCATTATTCGAAAACTTATCAGAATTTTCCTGATAGATTTTAGTTTTTTTTCTTTGAAAGTTCTTGTTGTTAATTTTTGTTTCAAAGCAGACTTTTCTTTGGCCCTCAGTTCGTTTCTGTTGAAAATTTTCAGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAACCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGGTAAAAATCTCCATCTCCGTCCTTATTTTTTTCCTGTTAACTATATGCTTAGATTGCAATTTCACGTTCTTTATAGAAACTCTTCTGTAGGACATTCTGTTCGCTGAAGTTCCAATTCTTGTTCATTGATTTTTTTCATGTTACCTTAGTGGCTTTTTGGGTCTATTCGTGCACTCAAGTTCTTAAATAGAACTAAAATTATCGAGCGAGTAACTTTTTTATCTTAAAACATTAATTTCTGTAGGGTATAACCATTAACTTGTATGCATCTTAAGTTTAGAAAATTGTTCGAAAACAGCCTATATAACACATGAAATCCAGCTTTAAAAAATCTAAACATCGATAAAAGAAACCAAATAAACGTTGTGTAACTAGGTAATTTTCATAAAAATAGTCTTGTTTCCCTTTAGAAATATTAATCAGCTTTTCTCTTCTTTTGCTCTTATTTTCTCGATGGAATTTTCCTCAATCATGTCCTTAGATGACACCATTTTTCTATCTTGTGATTCTTTGAATGGTCAGAATTATCAGAAATTCCAGAAATGAAGCTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCTTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGGTATTTTCCATAATTCTACCCTTTATCGCACATCGTGCAAAAATTTTACTTTCACCTTAGGATTGCCTGTTGAACTCCAGACATTTATAGTTCCCTTTAAGTTTCTAATTGCAAGTCTGAAGTGTTATGTAAGGCATATCTAGCACCATTTTTAATGATCCCATTCCCATTACTCAAAATCCAAACGTTTTCTGCATTGTGACTGTACACGTGCTGATAGAATCTGGTTGTTTTTGCCTCTCATTTGATATTATAACAACTACATGTGATGTGACCGGTCTACATCAAGTGGTTGCAATTATAAGAATAATGTTCGATTTCTGAAAGATAGTTTAAACAATTCTATATGCAAATTTACCTGTCATTTGACGTGTTTGTATTGTACATGCTCGACTAACTTACTGTGTGGTTGCTCTGTAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATAGTTAAGTACTCTAACCCCTGCCGTCTTCCACCTACGTTTTCCTTTCTATAGTTTGTCTCCATTTCCATATGCTCTGTATAGCTTTTATGTGCGTTCTTTTGTTGCTCTGTATTGCATTTTGGTACCACTTGTTTATGTGACATTGGTCGTAACGTTGCTTTTTCACGTACAACTATTGACAGGGAGATAATTTTGTACTGTGCTTTGCAGGTCACGTTGCTCACCTGAATATACATGACGAATTGCTTCCATATAAGGATGTTATTGCGAAGGTTATTTATGATGTATGTCATCAGTATCTATTTACTCATTTCCTAATTATTTTGAAATGTACACTGTGATTTATTGATCACTATAATTTAGCTGTTACTTCATTTTTTTATTCTCCCCTTATTTTCCTTCGTGCATATGTTGGGGGTACCTTTTTAAGAAATTCAAATGTTCACAATTGGATATTTAATATTTTTATCCTCCCCCTCCATCTAGCAATACACACACACACACACACACACATTTTCCCTATTGAAACTGTTTCTGTAACTATTTCTTTTTGTAATCTTCGCAGAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTGAAGCAATATGGAGCGACATTTAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTGTACAATTTGGATGCTAGGAAATTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAAACTGTCACCTCAATACTAAAAGCTTCTGAGGAAGGCATTGGAGGGACCAATCAAGAATCACCGTTGGCAAATGGTATGCTGACTGGTATTATATTATTATCATTTAAAGAAATTTTTATATCGTCTTCAGGAGATTAGTGTAGATGATCTTAATTTTCTTTCTTGCTTCAGTTCAGGTGCAAGAAGTTCAAGATATTGAAATTAGCAATAAATTTGTAGAACATTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCGGAAAGTTGTGAGAATGAGAAAGGTAACATACTGTACCATATCTTGTGATTGAACAACCAAGACCATTTCTTCCTATAATTTTCTTTGCAGATTTTTTTTTGTTTTGATGAAAATTTGCTTTGCAGACGACAGAATTGCTGATGATGCTTCCACCCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCACCAATGTATCTCATGCTAGGACCTGGGAACACATCGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGGTACGAATGGACTTTTTTCTTTTTAATTTCATTTCTCTTATTGCAGTTGTTTTAGCATATTTCTCTACATTTGTGTGTCGGAATGAAGAGTTGAAAGTTGGGGAAATAACAATGTTAGGAAAGGGAAACAATTACTTTTTAATGCTTCAGATATGAAGGGAAACCATAAACGATTCTTTTTCTCTTACTATCACCATGACAGACCATTTTGGATATGTCATATTTACTAATTCCTACCTCTCAAATGGAGTTTTGTTGTAAGTTTCGTAGTCACTCTCTATACCTATCTTACGAGTTTCTTCAATCTCCTTTAGCTCATGGAGAGTGCCTTATCTCCTCCGCTCAATTATCTCAACTCGTCTTTTGTATCCTTATCTATATTTTGCTTAGTTAACCAATGAAGTATATTTTGTTATTTTCTTGCCAGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAATCAAGATTTTATCATTTCGGTATGTTTATACTAATTACTATTGATCCTTATTTGTCTGTCTTCTTTTTGATAATTGTAATTCTTTCTCACCATTCTATTTGGATATTTGTCTCGAAAAAAGTCGTCTTTCTTCTCATACCATCCTCTTTTAACCGTTCCACTCCAGGTGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGTATTGCTCTGTGTGTGTGTATAAATGGCAACTATTTGGTTTTTAACTCATTTTTCTTTTTTGTTTTTTTATCAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGTGTCAATGAGGACACGACTAGCTAAAAATCGAGTAAAACTTTTCTATCTCTTGGGGCTTCATTTTCCGAATTTTTTTCCGCAGAGATTTTCTTGTTCTCACATCATCGTAATTCATGTTTGTTTCTCATTTCTGAGATGTACAAGGTAGACGTATGATATGGTAGGCTAGCGCTTGGGTATTTCAGTTCTAAAGTTGAGATTGGTGTTTAGCTGCAAAATGTGTTTGGAAAATTTTCCATATTGGCTCGGTATAATTCATAACTAATCACTTGAATTTGTATAAAACAAATGTCTCATGTCCTAAGTTATAACAATATCCCTTGATAGACTTAGTATGTGCCTTGATGAAAGTGCTTTTCTATTCTTTAACATCAAAATATGGTTATCAAAAAGGACATATTAATAAATAGCTGTTAAGTTTGTGTTAGGACTTTTGAGTTATAAATAGTAGGTGAGAGAGACTAG

mRNA sequence

GGTAACTAATTCCCAGTTCGAAATACTGTCTAAACGACGTCGTGTAGTGGTCTCCTAATTTTAGCTTAAACCCTAGTCCTGTCGCCGCCGGTGGTCACAGCAGTCTCCGGCGACAGTTCCCGCTGTTAGTCTTCTACTACGGTGAAAATCTCGATTCTGATTCTTTCTATCCGGTGGAAAAATCTCCGAAACCATTACTCTACATTGTTCTATTCTGATTTGAATTTGTTTGTATTGAATTCGGTGTAATTTTTGTTGTGTTATTGAGTATTGAGAAAAATCGAACTTGTGAATCGATCAATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCATTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAACCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAGCTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCTTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATAGTTAAGTACTCTAACCCCTGCCGTCACGTTGCTCACCTGAATATACATGACGAATTGCTTCCATATAAGGATGTTATTGCGAAGGTTATTTATGATAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTGAAGCAATATGGAGCGACATTTAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTGTACAATTTGGATGCTAGGAAATTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAAACTGTCACCTCAATACTAAAAGCTTCTGAGGAAGGCATTGGAGGGACCAATCAAGAATCACCGTTGGCAAATGTTCAGGTGCAAGAAGTTCAAGATATTGAAATTAGCAATAAATTTGTAGAACATTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCGGAAAGTTGTGAGAATGAGAAAGACGACAGAATTGCTGATGATGCTTCCACCCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCACCAATGTATCTCATGCTAGGACCTGGGAACACATCGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAATCAAGATTTTATCATTTCGGTGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGTGTCAATGAGGACACGACTAGCTAAAAATCGAGTAAAACTTTTCTATCTCTTGGGGCTTCATTTTCCGAATTTTTTTCCGCAGAGATTTTCTTGTTCTCACATCATCGTAATTCATGTTTGTTTCTCATTTCTGAGATGTACAAGGTAGACGTATGATATGGTAGGCTAGCGCTTGGGTATTTCAGTTCTAAAGTTGAGATTGGTGTTTAGCTGCAAAATGTGTTTGGAAAATTTTCCATATTGGCTCGGTATAATTCATAACTAATCACTTGAATTTGTATAAAACAAATGTCTCATGTCCTAAGTTATAACAATATCCCTTGATAGACTTAGTATGTGCCTTGATGAAAGTGCTTTTCTATTCTTTAACATCAAAATATGGTTATCAAAAAGGACATATTAATAAATAGCTGTTAAGTTTGTGTTAGGACTTTTGAGTTATAAATAGTAGGTGAGAGAGACTAG

Coding sequence (CDS)

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCATTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAACCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAGCTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCTTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATAGTTAAGTACTCTAACCCCTGCCGTCACGTTGCTCACCTGAATATACATGACGAATTGCTTCCATATAAGGATGTTATTGCGAAGGTTATTTATGATAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTGAAGCAATATGGAGCGACATTTAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTGTACAATTTGGATGCTAGGAAATTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAAACTGTCACCTCAATACTAAAAGCTTCTGAGGAAGGCATTGGAGGGACCAATCAAGAATCACCGTTGGCAAATGTTCAGGTGCAAGAAGTTCAAGATATTGAAATTAGCAATAAATTTGTAGAACATTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCGGAAAGTTGTGAGAATGAGAAAGACGACAGAATTGCTGATGATGCTTCCACCCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCACCAATGTATCTCATGCTAGGACCTGGGAACACATCGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAATCAAGATTTTATCATTTCGGTGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGTGTCAATGAGGACACGACTAGCTAA

Protein sequence

MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKVQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETIVKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS

Homology

[Close]

BLAST of Cmc05g0145221 vs. NCBI nr
Match: KAA0061573.1 (tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 985.7 bits (2547), Expect = 1.4e-283
Identity = 491/500 (98.20%), Postives = 491/500 (98.20%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPC--RHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180
                 C   HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE
Sbjct: 121 GDNFVLCFAGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180

Query: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240
           ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA
Sbjct: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240

Query: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300
           IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS
Sbjct: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300

Query: 301 ETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES 360
           ETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES
Sbjct: 301 ETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES 360

Query: 361 CENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD 420
           CENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD
Sbjct: 361 CENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD 420

Query: 421 AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK 480
           AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK
Sbjct: 421 AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK 480

Query: 481 AMYCLSFRLPEQCVNEDTTS 499
           AMYCLSFRLPEQCVNEDTTS
Sbjct: 481 AMYCLSFRLPEQCVNEDTTS 500

[Close]

BLAST of Cmc05g0145221 vs. NCBI nr
Match: XP_008458074.1 (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo])

HSP 1 Score: 983.8 bits (2542), Expect = 5.5e-283
Identity = 489/498 (98.19%), Postives = 490/498 (98.39%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180
                   HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL
Sbjct: 121 -------GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180

Query: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240
           KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP
Sbjct: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240

Query: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 300
           AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFIS+LMIVPPNESNSET
Sbjct: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSET 300

Query: 301 VTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCE 360
           VTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCE
Sbjct: 301 VTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCE 360

Query: 361 NEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAF 420
           NEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAF
Sbjct: 361 NEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAF 420

Query: 421 RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAM 480
           RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAM
Sbjct: 421 RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAM 480

Query: 481 YCLSFRLPEQCVNEDTTS 499
           YCLSFRLPEQCVNEDTTS
Sbjct: 481 YCLSFRLPEQCVNEDTTS 491

[Close]

BLAST of Cmc05g0145221 vs. NCBI nr
Match: XP_011648665.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 949.9 bits (2454), Expect = 8.8e-273
Identity = 476/499 (95.39%), Postives = 484/499 (96.99%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180
                   HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL
Sbjct: 121 -------GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180

Query: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240
           KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP
Sbjct: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240

Query: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 300
           AAQKECIVYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSE+
Sbjct: 241 AAQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSES 300

Query: 301 VTSILKASEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESC 360
           VTSILKA E+GIGGTNQESPLANVQVQEVQD I+ISNK VE SRSADISVAVLKRSSESC
Sbjct: 301 VTSILKACEKGIGGTNQESPLANVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESC 360

Query: 361 ENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDA 420
           ENEKD+ IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFLDA
Sbjct: 361 ENEKDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFLDA 420

Query: 421 FRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKA 480
           FRGSIKKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKA
Sbjct: 421 FRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKA 480

Query: 481 MYCLSFRLPEQCVNEDTTS 499
           MYCLSFRLPEQCV+EDTTS
Sbjct: 481 MYCLSFRLPEQCVDEDTTS 491

[Close]

BLAST of Cmc05g0145221 vs. NCBI nr
Match: XP_031736378.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 944.1 bits (2439), Expect = 4.8e-271
Identity = 476/503 (94.63%), Postives = 484/503 (96.22%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180
                   HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL
Sbjct: 121 -------GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180

Query: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240
           KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP
Sbjct: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240

Query: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 300
           AAQKECIVYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSE+
Sbjct: 241 AAQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSES 300

Query: 301 VTSILKASEEGIGGTNQESPLAN----VQVQEVQD-IEISNKFVEHSRSADISVAVLKRS 360
           VTSILKA E+GIGGTNQESPLAN    VQVQEVQD I+ISNK VE SRSADISVAVLKRS
Sbjct: 301 VTSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRS 360

Query: 361 SESCENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALH 420
           SESCENEKD+ IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALH
Sbjct: 361 SESCENEKDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALH 420

Query: 421 FLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVA 480
           FLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVA
Sbjct: 421 FLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVA 480

Query: 481 PNKAMYCLSFRLPEQCVNEDTTS 499
           PNKAMYCLSFRLPEQCV+EDTTS
Sbjct: 481 PNKAMYCLSFRLPEQCVDEDTTS 495

[Close]

BLAST of Cmc05g0145221 vs. NCBI nr
Match: TYK10701.1 (tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 934.5 bits (2414), Expect = 3.8e-268
Identity = 468/498 (93.98%), Postives = 469/498 (94.18%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAG-------------------- 120

Query: 121 VKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180
                   HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL
Sbjct: 121 --------HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180

Query: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240
           KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP
Sbjct: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240

Query: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 300
           AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET
Sbjct: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 300

Query: 301 VTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCE 360
           VTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCE
Sbjct: 301 VTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCE 360

Query: 361 NEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAF 420
           NEK+DRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAF
Sbjct: 361 NEKEDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAF 420

Query: 421 RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAM 480
           RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAM
Sbjct: 421 RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAM 470

Query: 481 YCLSFRLPEQCVNEDTTS 499
           YCLSFRLPEQCVNEDTTS
Sbjct: 481 YCLSFRLPEQCVNEDTTS 470

[Close]

BLAST of Cmc05g0145221 vs. ExPASy Swiss-Prot
Match: F6HH45 (tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0016g04350 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 3.9e-191
Identity = 339/504 (67.26%), Postives = 403/504 (79.96%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           +LDESKFD+ L LWALRIP +LCK A +ILNGYL+DKPR+KP+TEDPTC+ NRY+ILSE+
Sbjct: 2   LLDESKFDLQLKLWALRIPSQLCKVASRILNGYLLDKPRVKPITEDPTCETNRYIILSER 61

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           ++ P+LS+IPE K +ELK LC  E VPYSLTLGYSYW ADH+LK+ILP  VEVPSSFETI
Sbjct: 62  IKNPDLSDIPEDKRDELKVLCKIEVVPYSLTLGYSYWSADHVLKQILPDGVEVPSSFETI 121

Query: 121 VKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180
               + C H+AHLNI  ELLPYKDVIAKVIYDKNYPRIKTVVNKVG+ITNEFRVPKFEIL
Sbjct: 122 T--FSLC-HIAHLNITGELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEIL 181

Query: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240
            G++DMVTEVKQY ATFKLDYSLVYWNSRLEHEH+RLVS F+PG++ICDMF+G+GPFAIP
Sbjct: 182 VGKDDMVTEVKQYRATFKLDYSLVYWNSRLEHEHMRLVSQFRPGQIICDMFSGVGPFAIP 241

Query: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 300
           AAQK C+VYANDLNPDS+RYLK+NA+INKV   +  YN+DARKFISQLM VP +E   E 
Sbjct: 242 AAQKGCLVYANDLNPDSIRYLKINAKINKVDDNIWAYNMDARKFISQLMEVPVHEILPEP 301

Query: 301 VTSILKASEEGIGGTNQESPLAN----VQVQEVQDIEISN-KFVEHSRS-ADISVAVLKR 360
              +LKA+EE     N ++   N    V+   V   + SN   +E S + AD +VA +K+
Sbjct: 302 DVPVLKATEECSIQANVQTESQNGGLTVEAIGVHSDDSSNMDGIEGSCTVADDTVAAVKK 361

Query: 361 SSESCENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASAL 420
            S SCE E  +   D+A+  V GRR    NKRM+GS + S+ + WEH+DHVIMNLPASA+
Sbjct: 362 HSGSCEEE--NGTYDNATVSVVGRRKDRMNKRMRGSESSSNIKPWEHVDHVIMNLPASAI 421

Query: 421 HFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDV 480
            FLDAFRG I++KYWKGSLPWIHCYCF+RANE  + IIS AE+ALNA+IQ+PI HRVRDV
Sbjct: 422 QFLDAFRGLIQRKYWKGSLPWIHCYCFIRANETNEMIISEAETALNASIQEPILHRVRDV 481

Query: 481 APNKAMYCLSFRLPEQ-CVNEDTT 498
           APNKAM+CLSFRL E+ C  ED T
Sbjct: 482 APNKAMFCLSFRLSEEACFKEDAT 500

[Close]

BLAST of Cmc05g0145221 vs. ExPASy Swiss-Prot
Match: Q93YU6 (tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g56120 PE=2 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 7.7e-171
Identity = 305/497 (61.37%), Postives = 371/497 (74.65%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           V+  +LSEIPE KL +LK L   E VP+S+TLGYSYW ADH+LK+ILP  +++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 VKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180
                   H+AHLN+HDELLP+KDVIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPKFE+L
Sbjct: 121 -------GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVL 180

Query: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240
            GEN M TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIP
Sbjct: 181 AGENGMETEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIP 240

Query: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 300
           AAQK C VYANDLNPDSVRYLK+NA+ NKV   + V+N+DARKF S LM V   E N ++
Sbjct: 241 AAQKGCFVYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQS 300

Query: 301 VTSILKASEEGI--GGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES 360
           V    K  E  +  GG    S          ++I  SN  +     A+      K+ S +
Sbjct: 301 VADNDKTKEAAVSRGGETNSSG---------EEIRESNASINEPLGAN------KKPSGT 360

Query: 361 CENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD 420
            + E  + +  D  +       G+ NKR++  T +  A+ WEHIDHVIMNLPASAL FLD
Sbjct: 361 TKTE--NGVGKDCKS-----IEGHANKRLR-QTLLPIAKPWEHIDHVIMNLPASALQFLD 420

Query: 421 AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK 480
           +F   I+KKYWKG LP IHCYCF+RA+E  +FII+ AE+AL  +I+DP+FH+VRDVAPNK
Sbjct: 421 SFSNVIQKKYWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNK 467

Query: 481 AMYCLSFRLPEQCVNED 496
           AM+CLSFRLPE C+ ++
Sbjct: 481 AMFCLSFRLPEACLKQE 467

[Close]

BLAST of Cmc05g0145221 vs. ExPASy Swiss-Prot
Match: C5XX79 (tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g025390 PE=3 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 2.9e-154
Identity = 288/496 (58.06%), Postives = 353/496 (71.17%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKIL-NGYLIDKPRIKPVTEDPTCDKNRYVILSEK 61
           LDESKF+  L LWALRIPREL  A  ++L +GYL+DKPR+K V EDP  DKNR VILSEK
Sbjct: 4   LDESKFEQRLQLWALRIPRELSSAVTRLLRSGYLLDKPRVKTVVEDPEGDKNRLVILSEK 63

Query: 62  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 121
           +Q P+LS++P+ +L+ LK LC  + VPY+LTLGYSYW A                     
Sbjct: 64  IQKPDLSDMPQQELDSLKQLCNVDVVPYTLTLGYSYWSAG-------------------- 123

Query: 122 VKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 181
                   HVAHLNI D+LLPYK+VIAKVIYDKNYPRI+TV NKVG+ITNEFRVPKFEIL
Sbjct: 124 --------HVAHLNISDDLLPYKNVIAKVIYDKNYPRIQTVANKVGTITNEFRVPKFEIL 183

Query: 182 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 241
            G+NDMVTEVKQYGATFKLDY LVYWNSRL+HEHIRLVSLF+ G+VICDMFAGIGPFAIP
Sbjct: 184 AGKNDMVTEVKQYGATFKLDYGLVYWNSRLDHEHIRLVSLFKKGDVICDMFAGIGPFAIP 243

Query: 242 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSE- 301
           AAQK C+VYANDLNPDSV YL+ NA+INKV   +  YN+DAR F+  LM VP  E+ S+ 
Sbjct: 244 AAQKGCVVYANDLNPDSVHYLRTNAKINKVDDYIFAYNMDARVFMQNLMTVPGLETGSDC 303

Query: 302 TVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESC 361
            V +     +EG+      S  +N    +V+  E S      S  A  +    +++SE C
Sbjct: 304 QVAADESYPKEGVPANENSS--SNGNHNDVR--EGSQNGANESSVASTTAKKRQQTSEEC 363

Query: 362 ENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDA 421
           E++  D   D + T+   RRN   NKR++G      ++ WEH DHV+MNLPASAL FLD 
Sbjct: 364 ESDCQD--GDASQTK---RRN---NKRVRG-PGPPPSKPWEHFDHVLMNLPASALQFLDC 423

Query: 422 FRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKA 481
           F G ++KKYW GSLPWIHCYCF+R++E+++ I+S   + LNA I +PIFHRVRDVAPNKA
Sbjct: 424 FDGLVQKKYWTGSLPWIHCYCFIRSSESEESILS---NKLNAKIAEPIFHRVRDVAPNKA 455

Query: 482 MYCLSFRLPEQCVNED 496
           M+CLSF+LP +C+ ED
Sbjct: 484 MFCLSFKLPMECLRED 455

[Close]

BLAST of Cmc05g0145221 vs. ExPASy Swiss-Prot
Match: A9T6G5 (tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_141055 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.5e-113
Identity = 225/480 (46.88%), Postives = 297/480 (61.88%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           +D+S F V L   ALRIP++       +L+ YL+D PR+K + EDP     R ++ SE +
Sbjct: 11  IDKSAFVVRLPRVALRIPKQKSHRVASLLSKYLLDMPRVKHIVEDPQDPSMRLLLFSEAI 70

Query: 62  QTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETIV 121
             P+L  + E K+  +K +   + V + + L YSYW  +HILK+ILP   EVPSSFETI 
Sbjct: 71  AQPDLEGVSEEKILAVKEIVPLDVVQHEVVLDYSYWPVEHILKEILPAGCEVPSSFETI- 130

Query: 122 KYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILK 181
                  H+AHLN+ ++LL YK +IA+VI DKN P++KTVVNKVG+ITNEFRVP+FEIL 
Sbjct: 131 ------GHIAHLNLREDLLTYKKIIAEVILDKN-PKLKTVVNKVGTITNEFRVPEFEILA 190

Query: 182 GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA 241
           GE  +VTE+KQ+GATF+LDY +VYWNSRLE EH RL + F+PG+VI DMFAGIGPFAIPA
Sbjct: 191 GEPSLVTEIKQHGATFRLDYGMVYWNSRLEGEHKRLFAQFKPGQVIVDMFAGIGPFAIPA 250

Query: 242 AQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETV 301
           AQ+ C VYANDLNP SV++LK+N++INKV   +  +NLDAR+F+ +L  V   E     +
Sbjct: 251 AQQGCAVYANDLNPTSVKFLKLNSDINKVGESIKAFNLDAREFMRKL--VTEEEGCVGEI 310

Query: 302 TSILKASEEGIGGTNQESPLAN-VQVQEVQDIEISNKFVEH-SRSADISVAVLKRSSESC 361
            S    +E  + GT   S   N   + E  D+  S K  E  ++ A +S  V        
Sbjct: 311 ASSTLIAENSLNGTRIPSSKENSSSLTEGSDMPSSKKPEERKAKKAKVSEVV-------- 370

Query: 362 ENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDA 421
                                     +MK    +S  + WEH DHV+MNLPASAL FLD 
Sbjct: 371 --------------------------KMK----ISDVKPWEHFDHVVMNLPASALEFLDV 430

Query: 422 FRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKA 480
             G + K  WKG++P +HCYCFMR+NE    I+  AE+ L  +I DP  + VRDVAPNKA
Sbjct: 431 LNGLLCKGRWKGTMPRVHCYCFMRSNETNADIVKKAETYLGGSIADPDVYVVRDVAPNKA 442

[Close]

BLAST of Cmc05g0145221 vs. ExPASy Swiss-Prot
Match: F4NUJ6 (tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) OX=684364 GN=TRM5 PE=3 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 6.7e-74
Identity = 166/493 (33.67%), Postives = 260/493 (52.74%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           L+ + F+    L AL+IP      A++ L  +++  PR++ + +DPT  K R ++L   +
Sbjct: 16  LNHALFESSHTLPALKIPAHASGIAMETLRSHILVAPRLRTIVDDPTDKKWRLLLLDPSM 75

Query: 62  QTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETIV 121
           +  E++++P   L+E       + V +++ L Y YW +D +L+ ILP E+E P +FET+ 
Sbjct: 76  EASEINDLPN-PLKEFALKHEAKLVKHTIELKYDYWTSDQVLRSILPDEMETPGAFETV- 135

Query: 122 KYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILK 181
                  H+AHLN+ D   P+K +I +VI DK+   IKTVVNK+ +I + FR  + EIL 
Sbjct: 136 ------GHIAHLNLRDRYQPFKHIIGQVILDKS-SHIKTVVNKLDNIDHTFRFFQMEILA 195

Query: 182 GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA 241
           G NDM  ++K+ G  F  D+S VYWNSRL+ EH R++ LF   ++ICD+FAG+GPFA+PA
Sbjct: 196 GINDMNAKLKEGGCFFHFDFSKVYWNSRLQGEHDRIIKLFGQNDLICDVFAGVGPFALPA 255

Query: 242 AQ-KECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 301
           A+ K C+V+ANDLNP S +YL  N ++NK+  R+  +N+D R+FI Q             
Sbjct: 256 AKHKRCVVFANDLNPQSFKYLMENIKLNKLETRILPFNMDGRQFIKQ------------- 315

Query: 302 VTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCE 361
                                    ++++ +  I NK  +   +++      K+ +   E
Sbjct: 316 ------------------------SLEDLNNPAIWNKITKQKPTSND-----KKRNRKVE 375

Query: 362 NEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAF 421
           +     + D  +  ++G R                     H  H +MNLPA+A+ FLDAF
Sbjct: 376 SPTVAPLTDQPA--ISGIR---------------------HFKHYVMNLPATAIEFLDAF 434

Query: 422 RG---SIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIF--HRVRDVA 481
            G    ++       LP IHC+CF  A + +  +I   E  +   +   +   H VR VA
Sbjct: 436 HGLYSGMRDVIMDSDLPTIHCHCFSNAKDVKADVIERVERVIGMPLGSNLIMVHSVRTVA 434

Query: 482 PNKAMYCLSFRLP 489
           PNK M C+SFRLP
Sbjct: 496 PNKDMLCISFRLP 434

[Close]

BLAST of Cmc05g0145221 vs. ExPASy TrEMBL
Match: A0A5A7V0B1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001350 PE=3 SV=1)

HSP 1 Score: 985.7 bits (2547), Expect = 7.0e-284
Identity = 491/500 (98.20%), Postives = 491/500 (98.20%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPC--RHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180
                 C   HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE
Sbjct: 121 GDNFVLCFAGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180

Query: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240
           ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA
Sbjct: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240

Query: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300
           IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS
Sbjct: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300

Query: 301 ETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES 360
           ETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES
Sbjct: 301 ETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES 360

Query: 361 CENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD 420
           CENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD
Sbjct: 361 CENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD 420

Query: 421 AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK 480
           AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK
Sbjct: 421 AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK 480

Query: 481 AMYCLSFRLPEQCVNEDTTS 499
           AMYCLSFRLPEQCVNEDTTS
Sbjct: 481 AMYCLSFRLPEQCVNEDTTS 500

[Close]

BLAST of Cmc05g0145221 vs. ExPASy TrEMBL
Match: A0A1S3C7L3 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 PE=3 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 2.7e-283
Identity = 489/498 (98.19%), Postives = 490/498 (98.39%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180
                   HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL
Sbjct: 121 -------GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180

Query: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240
           KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP
Sbjct: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240

Query: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 300
           AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFIS+LMIVPPNESNSET
Sbjct: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSET 300

Query: 301 VTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCE 360
           VTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCE
Sbjct: 301 VTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCE 360

Query: 361 NEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAF 420
           NEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAF
Sbjct: 361 NEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAF 420

Query: 421 RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAM 480
           RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAM
Sbjct: 421 RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAM 480

Query: 481 YCLSFRLPEQCVNEDTTS 499
           YCLSFRLPEQCVNEDTTS
Sbjct: 481 YCLSFRLPEQCVNEDTTS 491

[Close]

BLAST of Cmc05g0145221 vs. ExPASy TrEMBL
Match: A0A0A0LFW8 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G005350 PE=3 SV=1)

HSP 1 Score: 950.3 bits (2455), Expect = 3.3e-273
Identity = 476/499 (95.39%), Postives = 485/499 (97.19%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 VKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180
           +       HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL
Sbjct: 121 L-----LCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180

Query: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240
           KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP
Sbjct: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240

Query: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 300
           AAQKECIVYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSE+
Sbjct: 241 AAQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSES 300

Query: 301 VTSILKASEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESC 360
           VTSILKA E+GIGGTNQESPLANVQVQEVQD I+ISNK VE SRSADISVAVLKRSSESC
Sbjct: 301 VTSILKACEKGIGGTNQESPLANVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESC 360

Query: 361 ENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDA 420
           ENEKD+ IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFLDA
Sbjct: 361 ENEKDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFLDA 420

Query: 421 FRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKA 480
           FRGSIKKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKA
Sbjct: 421 FRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKA 480

Query: 481 MYCLSFRLPEQCVNEDTTS 499
           MYCLSFRLPEQCV+EDTTS
Sbjct: 481 MYCLSFRLPEQCVDEDTTS 493

[Close]

BLAST of Cmc05g0145221 vs. ExPASy TrEMBL
Match: A0A5D3CFY1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00100 PE=3 SV=1)

HSP 1 Score: 934.5 bits (2414), Expect = 1.9e-268
Identity = 468/498 (93.98%), Postives = 469/498 (94.18%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAG-------------------- 120

Query: 121 VKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180
                   HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL
Sbjct: 121 --------HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180

Query: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240
           KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP
Sbjct: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240

Query: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 300
           AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET
Sbjct: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 300

Query: 301 VTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCE 360
           VTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCE
Sbjct: 301 VTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCE 360

Query: 361 NEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAF 420
           NEK+DRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAF
Sbjct: 361 NEKEDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAF 420

Query: 421 RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAM 480
           RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAM
Sbjct: 421 RGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAM 470

Query: 481 YCLSFRLPEQCVNEDTTS 499
           YCLSFRLPEQCVNEDTTS
Sbjct: 481 YCLSFRLPEQCVNEDTTS 470

[Close]

BLAST of Cmc05g0145221 vs. ExPASy TrEMBL
Match: A0A6J1DUW2 (tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111024249 PE=3 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 4.9e-245
Identity = 425/495 (85.86%), Postives = 453/495 (91.52%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHL LWALRIPRELCKAAIKILNGYLID+ R+KPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKAAIKILNGYLIDRARVKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQ PELS+IPE KLEELKGLC  E VPYSLTLGYSYWGADHILK+ILPP VEVPSSFETI
Sbjct: 61  VQAPELSQIPEAKLEELKGLCEIELVPYSLTLGYSYWGADHILKQILPPRVEVPSSFETI 120

Query: 121 VKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180
                   H+AHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVG+ITN+FRVPKFEIL
Sbjct: 121 -------GHIAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNKFRVPKFEIL 180

Query: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240
            GE+DMVTEVKQYGATFKLDY LVYWNSRLEHEH+RLVSLFQPGE+ICDMFAGIGPFAIP
Sbjct: 181 MGEHDMVTEVKQYGATFKLDYGLVYWNSRLEHEHVRLVSLFQPGEIICDMFAGIGPFAIP 240

Query: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 300
           AAQKECIVY NDLNPDSV+YLKVNAEINKVS RVHVYNLDARKFISQLMIVP NESNSE+
Sbjct: 241 AAQKECIVYGNDLNPDSVQYLKVNAEINKVSDRVHVYNLDARKFISQLMIVPDNESNSES 300

Query: 301 VTSILKASEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESC 360
           VTS+LKA ++GIGG+NQESP ANVQV+EVQ+  +IS++ VEHSRSADISV+VLKRSSESC
Sbjct: 301 VTSVLKACDKGIGGSNQESPSANVQVEEVQENSKISDETVEHSRSADISVSVLKRSSESC 360

Query: 361 ENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDA 420
             EKDD I DDAS  VAGR+  N NKRMKGS N+ HARTWEH+DHVIMNLPASAL FLDA
Sbjct: 361 --EKDDGIVDDASISVAGRKKRNANKRMKGS-NICHARTWEHVDHVIMNLPASALQFLDA 420

Query: 421 FRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKA 480
           FRGSI++KYWKGSLPWIHCYCFMRANENQDFIIS AESALN  IQDP+FHRVRDVAPNKA
Sbjct: 421 FRGSIRRKYWKGSLPWIHCYCFMRANENQDFIISEAESALNGKIQDPVFHRVRDVAPNKA 480

Query: 481 MYCLSFRLPEQCVNE 495
           MYCLSFRLPEQC NE
Sbjct: 481 MYCLSFRLPEQCFNE 485

[Close]

BLAST of Cmc05g0145221 vs. TAIR 10
Match: AT3G56120.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 601.7 bits (1550), Expect = 5.5e-172
Identity = 305/497 (61.37%), Postives = 371/497 (74.65%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           V+  +LSEIPE KL +LK L   E VP+S+TLGYSYW ADH+LK+ILP  +++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 VKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEIL 180
                   H+AHLN+HDELLP+KDVIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPKFE+L
Sbjct: 121 -------GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVL 180

Query: 181 KGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIP 240
            GEN M TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIP
Sbjct: 181 AGENGMETEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIP 240

Query: 241 AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSET 300
           AAQK C VYANDLNPDSVRYLK+NA+ NKV   + V+N+DARKF S LM V   E N ++
Sbjct: 241 AAQKGCFVYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQS 300

Query: 301 VTSILKASEEGI--GGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES 360
           V    K  E  +  GG    S          ++I  SN  +     A+      K+ S +
Sbjct: 301 VADNDKTKEAAVSRGGETNSSG---------EEIRESNASINEPLGAN------KKPSGT 360

Query: 361 CENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD 420
            + E  + +  D  +       G+ NKR++  T +  A+ WEHIDHVIMNLPASAL FLD
Sbjct: 361 TKTE--NGVGKDCKS-----IEGHANKRLR-QTLLPIAKPWEHIDHVIMNLPASALQFLD 420

Query: 421 AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK 480
           +F   I+KKYWKG LP IHCYCF+RA+E  +FII+ AE+AL  +I+DP+FH+VRDVAPNK
Sbjct: 421 SFSNVIQKKYWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNK 467

Query: 481 AMYCLSFRLPEQCVNED 496
           AM+CLSFRLPE C+ ++
Sbjct: 481 AMFCLSFRLPEACLKQE 467

[Close]

BLAST of Cmc05g0145221 vs. TAIR 10
Match: AT4G27340.1 (Met-10+ like family protein )

HSP 1 Score: 203.0 bits (515), Expect = 5.7e-52
Identity = 105/266 (39.47%), Postives = 160/266 (60.15%), Query Frame = 0


Query: 53  RYVILSEKVQTPELSEIPE------MKLEELKGLCMFERVPYSLTLGYSYWGADHILKKI 112
           R ++L EK    E+ ++PE       + +       FE V   LTL Y YW    IL+ +
Sbjct: 274 RLLLLDEKYSGEEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAV 333

Query: 113 LPPEVEVPSSFETIVKYSNPCRHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVG 172
           LP  + VPS+FE +        H+AHLN+ DE LPYK +IAKV+ DKN P+I+TVVNK+ 
Sbjct: 334 LPKGMIVPSAFEMV-------GHIAHLNLRDEHLPYKRLIAKVVLDKNQPKIQTVVNKID 393

Query: 173 SITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEV 232
            I N+FR  + E+L G + +VT V + G  F +D + VYWNS+L  E  RL+  F   +V
Sbjct: 394 PIHNDFRTMQLEVLAGNHSLVTLVVENGLRFHVDLARVYWNSKLGTERQRLLLGFDQNDV 453

Query: 233 ICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFIS 292
           +CD+FAG+GP A+ AA+    VYANDLNP +V +++ N+ +NK+  R+ ++N+D R+FI 
Sbjct: 454 VCDVFAGVGPIALAAARIVKRVYANDLNPHAVEFMEQNSVVNKLEKRIEIFNMDGRRFIK 513

Query: 293 QLMIVPPNESNSETVTSILKASEEGI 313
            +      +  ++ V ++ K + E +
Sbjct: 514 AMFSSEKGQKVTQVVMNLPKDAAESL 532

[Close]

BLAST of Cmc05g0145221 vs. TAIR 10
Match: AT4G04670.1 (Met-10+ like family protein / kelch repeat-containing protein )

HSP 1 Score: 55.5 bits (132), Expect = 1.5e-07
Identity = 51/193 (26.42%), Postives = 87/193 (45.08%), Query Frame = 0


Query: 170 NEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICD 229
           N  R    EIL G+N  V   ++ G  +  D +   ++     E +R+ ++    EV+ D
Sbjct: 785 NGTRDSTLEILVGDNGWVNH-RENGILYSFDATKCMFSWGNLSEKLRMGNMACENEVVVD 844

Query: 230 MFAGIGPFAIP--AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDAR----K 289
           +FAGIG F +P     K  +VYA + NP ++  L+ N E N VS R  +   D R    K
Sbjct: 845 LFAGIGYFVLPFLVRAKAKLVYACEWNPHAIEALRRNVEANSVSERCIILEGDNRITAPK 904

Query: 290 FIS---QLMIVPPNESNSETVTSIL-------------KASEEGIGGTNQESPLANVQVQ 341
            ++    L ++P +E +  T    L             K S+E   G +    L+++   
Sbjct: 905 GVADRVNLGLIPSSEGSWVTAIQALRPEGGILHVHGNVKDSDESSWGEHVTKTLSDIARA 964

The following BLAST results are available for this feature:

BLAST of Cmc05g0145221 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0061573.1	1.4e-283	98.20	tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa]	[more]
XP_008458074.1	5.5e-283	98.19	PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo]	[more]
XP_011648665.1	8.8e-273	95.39	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucumis sativus]	[more]
XP_031736378.1	4.8e-271	94.63	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucumis sativus]	[more]
TYK10701.1	3.8e-268	93.98	tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa]	[more]

BLAST of Cmc05g0145221 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F6HH45	3.9e-191	67.26	tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0...	[more]
Q93YU6	7.7e-171	61.37	tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3...	[more]
C5XX79	2.9e-154	58.06	tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g02539...	[more]
A9T6G5	1.5e-113	46.88	tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPA...	[more]
F4NUJ6	6.7e-74	33.67	tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strai...	[more]

BLAST of Cmc05g0145221 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7V0B1	7.0e-284	98.20	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3C7L3	2.7e-283	98.19	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 ...	[more]
A0A0A0LFW8	3.3e-273	95.39	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G0053...	[more]
A0A5D3CFY1	1.9e-268	93.98	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1DUW2	4.9e-245	85.86	tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of Cmc05g0145221 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56120.1	5.5e-172	61.37	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G27340.1	5.7e-52	39.47	Met-10+ like family protein	[more]
AT4G04670.1	1.5e-07	26.42	Met-10+ like family protein / kelch repeat-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PFAM	PF02475	Met_10	coord: 129..439 e-value: 8.8E-63 score: 211.7
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PROSITE	PS51684	SAM_MT_TRM5_TYW2	coord: 117..489 score: 102.920769
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 187..487 e-value: 1.7E-57 score: 196.0
None	No IPR available	GENE3D	3.30.300.110		coord: 100..184 e-value: 7.0E-19 score: 69.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 363..392
None	No IPR available	PANTHER	PTHR23245:SF36	TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE	coord: 2..493
None	No IPR available	PANTHER	PTHR23245	TRNA METHYLTRANSFERASE	coord: 2..493
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 227..284 e-value: 3.96953E-4 score: 37.7947
IPR025792	tRNA (guanine(37)-N(1))-methyltransferase, eukaryotic	HAMAP	MF_03152	TRM5	coord: 2..489 score: 25.01199
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 125..489

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc05g0145221.1	Cmc05g0145221.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0005759	mitochondrial matrix
cellular_component	GO:0005634	nucleus
molecular_function	GO:0052906	tRNA (guanine(37)-N(1))-methyltransferase activity
molecular_function	GO:0009019	tRNA (guanine-N1-)-methyltransferase activity