Cmc05g0140511 (gene) Melon (Charmono) v1.1

Overview
NameCmc05g0140511
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Descriptionnicotianamine synthase
LocationCMiso1.1chr05: 24903674 .. 24904513 (-)
RNA-Seq ExpressionCmc05g0140511
SyntenyCmc05g0140511
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTTGCCAAAGTCTTCTTTTGGTCCAAAAAATCTCAGAGCTTTACCACAAGATCTCCACTCTTGAGAGCCTCAAACCCTCTAAACATGTCAACTCCCTCTTCTCTCAACTTGTACTCCTCATTTGCACTCCCCCGATGGCCTCCTCTTCTCCCTCGTCCATCGACGTCTCGTCGCTCTGCTCCTCCATCCAAACCATGAGATCCAATCTCATCAAGCTCTGCGGCGAGGCCGAGTCCCTTCTCGAGCATCACTACTCCACCATTTTATCTTCCCACCAAAACCCTCTCGACCATCTCTCCCTTTTCCCTTATTACTCCAACTACCTCAAACTAACTCACTTAGAATTCTCCATTTTGAACCACCACATTACGCCGCCTCCCTCTAAAGTGGCATTTGTTGGCTCCGGTGCACTTCCCTTCTCGTCTATCATCATGGCCTTGAAGTACCTCCCGAAAACCGAGTTTCACAACTTCGATCTCGACCCATCTGCCAATGCCAAAGCATCGATGCTGGTAGCAGGCGACGTGGAGCTGTCGAGGCGAATGGTGTTCCACACGACCGACATCATGGAGGTTGGGTCGGAAGAGTTGAGGGAGTTCGAGGTGGTGTTTTTGGCGGCGTTGGTGGGAATGGAGAGAGAAGAGAAGGCGAAGGTGATCGAGCATTTAAGGAAGAATATGAGGGGTGGGGCGTTGTTGATGTTGAGGAGCGCATATGGTGCACGGGCCTTTCTTTATCCGGTGGTGGAAGCTTGTGATCTTCGGGGGTTCGAGATTCTTACGGTGTTTCATCCAACGGATGAAGTTATTAATTCGATTGTGTTGGCACGATTATAA

mRNA sequence

ATGTGTTGCCAAAGTCTTCTTTTGGTCCAAAAAATCTCAGAGCTTTACCACAAGATCTCCACTCTTGAGAGCCTCAAACCCTCTAAACATGTCAACTCCCTCTTCTCTCAACTTGTACTCCTCATTTGCACTCCCCCGATGGCCTCCTCTTCTCCCTCGTCCATCGACGTCTCGTCGCTCTGCTCCTCCATCCAAACCATGAGATCCAATCTCATCAAGCTCTGCGGCGAGGCCGAGTCCCTTCTCGAGCATCACTACTCCACCATTTTATCTTCCCACCAAAACCCTCTCGACCATCTCTCCCTTTTCCCTTATTACTCCAACTACCTCAAACTAACTCACTTAGAATTCTCCATTTTGAACCACCACATTACGCCGCCTCCCTCTAAAGTGGCATTTGTTGGCTCCGGTGCACTTCCCTTCTCGTCTATCATCATGGCCTTGAAGTACCTCCCGAAAACCGAGTTTCACAACTTCGATCTCGACCCATCTGCCAATGCCAAAGCATCGATGCTGGTAGCAGGCGACGTGGAGCTGTCGAGGCGAATGGTGTTCCACACGACCGACATCATGGAGGTTGGGTCGGAAGAGTTGAGGGAGTTCGAGGTGGTGTTTTTGGCGGCGTTGGTGGGAATGGAGAGAGAAGAGAAGGCGAAGGTGATCGAGCATTTAAGGAAGAATATGAGGGGTGGGGCGTTGTTGATGTTGAGGAGCGCATATGGTGCACGGGCCTTTCTTTATCCGGTGGTGGAAGCTTGTGATCTTCGGGGGTTCGAGATTCTTACGGTGTTTCATCCAACGGATGAAGTTATTAATTCGATTGTGTTGGCACGATTATAA

Coding sequence (CDS)

ATGTGTTGCCAAAGTCTTCTTTTGGTCCAAAAAATCTCAGAGCTTTACCACAAGATCTCCACTCTTGAGAGCCTCAAACCCTCTAAACATGTCAACTCCCTCTTCTCTCAACTTGTACTCCTCATTTGCACTCCCCCGATGGCCTCCTCTTCTCCCTCGTCCATCGACGTCTCGTCGCTCTGCTCCTCCATCCAAACCATGAGATCCAATCTCATCAAGCTCTGCGGCGAGGCCGAGTCCCTTCTCGAGCATCACTACTCCACCATTTTATCTTCCCACCAAAACCCTCTCGACCATCTCTCCCTTTTCCCTTATTACTCCAACTACCTCAAACTAACTCACTTAGAATTCTCCATTTTGAACCACCACATTACGCCGCCTCCCTCTAAAGTGGCATTTGTTGGCTCCGGTGCACTTCCCTTCTCGTCTATCATCATGGCCTTGAAGTACCTCCCGAAAACCGAGTTTCACAACTTCGATCTCGACCCATCTGCCAATGCCAAAGCATCGATGCTGGTAGCAGGCGACGTGGAGCTGTCGAGGCGAATGGTGTTCCACACGACCGACATCATGGAGGTTGGGTCGGAAGAGTTGAGGGAGTTCGAGGTGGTGTTTTTGGCGGCGTTGGTGGGAATGGAGAGAGAAGAGAAGGCGAAGGTGATCGAGCATTTAAGGAAGAATATGAGGGGTGGGGCGTTGTTGATGTTGAGGAGCGCATATGGTGCACGGGCCTTTCTTTATCCGGTGGTGGAAGCTTGTGATCTTCGGGGGTTCGAGATTCTTACGGTGTTTCATCCAACGGATGAAGTTATTAATTCGATTGTGTTGGCACGATTATAA

Protein sequence

MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSLCSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL
Homology
BLAST of Cmc05g0140511 vs. NCBI nr
Match: XP_008453494.1 (PREDICTED: nicotianamine synthase 1-like [Cucumis melo])

HSP 1 Score: 538.9 bits (1387), Expect = 2.6e-149
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0

Query: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60
           MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60

Query: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120
           CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL
Sbjct: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120

Query: 121 NHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELS 180
           NHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELS
Sbjct: 121 NHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELS 180

Query: 181 RRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAY 240
           RRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAY
Sbjct: 181 RRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAY 240

Query: 241 GARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 280
           GARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL
Sbjct: 241 GARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 279

BLAST of Cmc05g0140511 vs. NCBI nr
Match: XP_004144812.1 (nicotianamine synthase 1 [Cucumis sativus])

HSP 1 Score: 497.7 bits (1280), Expect = 6.7e-137
Identity = 262/283 (92.58%), Postives = 268/283 (94.70%), Query Frame = 0

Query: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60
           MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTP     S SSIDVS+L
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTP-----SSSSIDVSTL 60

Query: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120
           CS+IQTMRS+LIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL
Sbjct: 61  CSTIQTMRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120

Query: 121 NHH----ITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGD 180
           NHH     T PPSKVAFVGSGALPFSSIIMALKY PKTEFHNFDLDPSANAKASMLVAGD
Sbjct: 121 NHHAPHSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGD 180

Query: 181 VELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLML 240
           VELSRRMVFHTTDIM+VGSEELREFEVVFLAALVGMEREEK KVIEHLRKNM GGALLML
Sbjct: 181 VELSRRMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLML 240

Query: 241 RSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 280
           RSAYGARAF+YPVVEACDLRGF+ILTVFHPTDEVINSIVLARL
Sbjct: 241 RSAYGARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 278

BLAST of Cmc05g0140511 vs. NCBI nr
Match: XP_038891237.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 478.4 bits (1230), Expect = 4.2e-131
Identity = 251/280 (89.64%), Postives = 265/280 (94.64%), Query Frame = 0

Query: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60
           MCCQ LLLVQKISELYHKISTLESLKPSK VNSLF+QLV LIC+PP   SSPSSIDVS+L
Sbjct: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFTQLV-LICSPP--PSSPSSIDVSTL 60

Query: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120
           CS++QTMRS+LIKLCGEAE+LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEFSIL
Sbjct: 61  CSAVQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120

Query: 121 NHHIT-PPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVEL 180
           NHH    PPSKVAFVGSGALPFSSIIMALKYLPKTE HNFD+DPSANAKA+MLVAGD EL
Sbjct: 121 NHHAPHAPPSKVAFVGSGALPFSSIIMALKYLPKTELHNFDIDPSANAKAAMLVAGDAEL 180

Query: 181 SRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSA 240
           S+RMVFHTTDIM+V S+ELREFEVVFLAALVGMEREEK KVIEHLRK+M GGALLMLRSA
Sbjct: 181 SKRMVFHTTDIMDVASDELREFEVVFLAALVGMEREEKVKVIEHLRKSMGGGALLMLRSA 240

Query: 241 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 280
           YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL
Sbjct: 241 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 277

BLAST of Cmc05g0140511 vs. NCBI nr
Match: KAA0048068.1 (nicotianamine synthase 1-like [Cucumis melo var. makuwa] >TYJ96468.1 nicotianamine synthase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 452.6 bits (1163), Expect = 2.5e-123
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0

Query: 47  MASSSPSSIDVSSLCSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYY 106
           MASSSPSSIDVSSLCSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYY
Sbjct: 1   MASSSPSSIDVSSLCSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYY 60

Query: 107 SNYLKLTHLEFSILNHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSAN 166
           SNYLKLTHLEFSILNHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSAN
Sbjct: 61  SNYLKLTHLEFSILNHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSAN 120

Query: 167 AKASMLVAGDVELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRK 226
           AKASMLVAGDVELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRK
Sbjct: 121 AKASMLVAGDVELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRK 180

Query: 227 NMRGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 280
           NMRGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL
Sbjct: 181 NMRGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 233

BLAST of Cmc05g0140511 vs. NCBI nr
Match: XP_022965879.1 (nicotianamine synthase [Cucurbita maxima])

HSP 1 Score: 438.7 bits (1127), Expect = 3.7e-119
Identity = 229/282 (81.21%), Postives = 251/282 (89.01%), Query Frame = 0

Query: 1   MCC--QSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVS 60
           MCC  + LLLVQK+SELYHKISTL+SL+PSKHVNSLFSQLVL    PP    SP  ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  SLCSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFS 120
            L S++QTMRS+LIKLCG+AE LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILNHHITPP-PSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDV 180
           IL  +I+P  PSKVAF+GSGALPFSSIIMALKYLP TEFHNFD+D SANAKA+MLVA DV
Sbjct: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180

Query: 181 ELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLR 240
           +LSRRM FHTTDIM+V S+ LREFEVVFLAALVGME+EEK KVIEHLRKNM  G+LLMLR
Sbjct: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 280
           SAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

BLAST of Cmc05g0140511 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 2.3e-84
Identity = 160/281 (56.94%), Postives = 219/281 (77.94%), Query Frame = 0

Query: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60
           M CQ+ L+V++I +LY +IS L+SLKPSK+V++LF QLV   C P     + ++IDV+++
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLV-STCLP-----TDTNIDVTNM 60

Query: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSS---HQNPLDHLSLFPYYSNYLKLTHLEF 120
           C  ++ MR+NLIKLCGEAE  LE H+STIL S    QNPLDHL +FPYYSNYLKL  LEF
Sbjct: 61  CEEVKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEF 120

Query: 121 SILNHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDV 180
            +L+ H +  P+K+AFVGSG +P +SI++A  +LP T FHNFD+D  AN  AS LV+ D 
Sbjct: 121 DLLSQHSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDP 180

Query: 181 ELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLR 240
           +LS+RM+FHTTD++   +E L +++VVFLAALVGM++E K K IEHL K+M  GA+LMLR
Sbjct: 181 DLSKRMIFHTTDVLN-ATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 279
           SA+  RAFLYP+V++ DL+GF++LT++HPTD+V+NS+V+AR
Sbjct: 241 SAHALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

BLAST of Cmc05g0140511 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 6.8e-84
Identity = 166/278 (59.71%), Postives = 214/278 (76.98%), Query Frame = 0

Query: 3   CQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSLCS 62
           CQ   LV KI +LY KIS LE+LKP + V++LF QLV   C PP       +IDV+ +  
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLV-STCIPP-----NPNIDVTKMSE 63

Query: 63  SIQTMRSNLIKLCGEAESLLEHHYSTILSSHQ-NPLDHLSLFPYYSNYLKLTHLEFSILN 122
           +IQ MRSNLIK+CGEAE  LEHH+S+IL+S + NPL HL+LFPYY+NYLKL+ LEF +L 
Sbjct: 64  NIQEMRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLE 123

Query: 123 HHITP-PPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELS 182
            ++    P  VAF+GSG LP +S+++A  +L  + FHNFD+DPSAN  A+ LV+ D +LS
Sbjct: 124 QNLNGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLS 183

Query: 183 RRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAY 242
           +RM FHT DIM+V +E L+ F+VVFLAALVGM+++EK KV+EHL K+M  GALLMLRSA+
Sbjct: 184 QRMFFHTVDIMDV-TESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAH 243

Query: 243 GARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 279
           G RAFLYP+VE CDL GFE+L+V+HPTDEVINSIV++R
Sbjct: 244 GPRAFLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

BLAST of Cmc05g0140511 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.2e-83
Identity = 165/280 (58.93%), Postives = 216/280 (77.14%), Query Frame = 0

Query: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60
           M CQ   LVQ I +LY KIS LESLKPS+ VN LF QLV   C PP       +IDV+ +
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLV-STCIPP-----NPNIDVTKM 60

Query: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQ-NPLDHLSLFPYYSNYLKLTHLEFSI 120
           C  +Q +R NLIK+CG AE  LE+H+S+IL+S+Q NPL HL++FPYY+NYLKL  LEF +
Sbjct: 61  CDRVQEIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDL 120

Query: 121 LNHHITP-PPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVE 180
           L  ++    P  VAF+GSG LP +SI++A  +L  T FHNFD+DPSAN+ AS+LV+ D +
Sbjct: 121 LEQNLNGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPD 180

Query: 181 LSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRS 240
           +S+RM FHT DIM+V +E L+ F+VVFLAALVGM +EEK KVIEHL+K+M  GA+LMLRS
Sbjct: 181 ISQRMFFHTVDIMDV-TESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRS 240

Query: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 279
           A+G RAFLYP+VE CDL+GFE+L+++HPTD+VINS+V+++
Sbjct: 241 AHGPRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

BLAST of Cmc05g0140511 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 3.7e-82
Identity = 159/278 (57.19%), Postives = 212/278 (76.26%), Query Frame = 0

Query: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60
           +C  S  +V+K+ ELY +IS LE+L PSK VN LF+ LV   C P      P+ IDVS L
Sbjct: 2   VCPNSNPVVEKVCELYEQISRLENLSPSKDVNVLFTDLV-HTCMP------PNPIDVSKL 61

Query: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120
           C  IQ +RS+LIKLCG+AE LLE H+S ILSS++NPL HL +FPY+ NY+KL+ LE++IL
Sbjct: 62  CQKIQEIRSHLIKLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNIL 121

Query: 121 NHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELS 180
             + T  P K+AF+GSG LP +S+++A K+L  T FHN+D+D  AN  AS LVA D ++S
Sbjct: 122 TKNTTNIPKKIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMS 181

Query: 181 RRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAY 240
            RM FHT D+M+V +  L++++VVFLAALVGM++E+K KV++HL K M  GA LMLRSA+
Sbjct: 182 SRMTFHTADVMDV-TCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAH 241

Query: 241 GARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 279
           GARAFLYPV++  DLRGFE+L+V+HPTDEVINS+++AR
Sbjct: 242 GARAFLYPVLDPRDLRGFEVLSVYHPTDEVINSVIIAR 271

BLAST of Cmc05g0140511 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 4.9e-82
Identity = 157/280 (56.07%), Postives = 218/280 (77.86%), Query Frame = 0

Query: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60
           M C++ L+V++I +LY++IS LESLKPSK+V++LF QLV   C P     + ++IDV+ +
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLV-STCLP-----TDTNIDVTEI 60

Query: 61  -CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQ-NPLDHLSLFPYYSNYLKLTHLEFS 120
               ++ MRS+LIKLCGEAE  LE H+S IL S + NPL+HL +FPYY+NYLKL  LEF 
Sbjct: 61  HDEKVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFD 120

Query: 121 ILNHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVE 180
           +L+ H T  P+KVAF+GSG +P +SI++A  +LP T FHNFD+D  AN  AS LV+ D +
Sbjct: 121 LLSQHTTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSD 180

Query: 181 LSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRS 240
           LS+RM+FHTTD++    E L +++VVFLAALVGM++E K K IEHL K+M  GA++MLRS
Sbjct: 181 LSKRMIFHTTDVLN-AKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRS 240

Query: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 279
           A+G RAFLYP+V++CDL+GFE+LT++HP+D+V+NS+V+AR
Sbjct: 241 AHGLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

BLAST of Cmc05g0140511 vs. ExPASy TrEMBL
Match: A0A1S3BXJ6 (nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.3e-149
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0

Query: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60
           MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60

Query: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120
           CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL
Sbjct: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120

Query: 121 NHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELS 180
           NHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELS
Sbjct: 121 NHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELS 180

Query: 181 RRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAY 240
           RRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAY
Sbjct: 181 RRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAY 240

Query: 241 GARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 280
           GARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL
Sbjct: 241 GARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 279

BLAST of Cmc05g0140511 vs. ExPASy TrEMBL
Match: A0A0A0LJ09 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.2e-137
Identity = 262/283 (92.58%), Postives = 268/283 (94.70%), Query Frame = 0

Query: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60
           MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTP     S SSIDVS+L
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTP-----SSSSIDVSTL 60

Query: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120
           CS+IQTMRS+LIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL
Sbjct: 61  CSTIQTMRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120

Query: 121 NHH----ITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGD 180
           NHH     T PPSKVAFVGSGALPFSSIIMALKY PKTEFHNFDLDPSANAKASMLVAGD
Sbjct: 121 NHHAPHSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGD 180

Query: 181 VELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLML 240
           VELSRRMVFHTTDIM+VGSEELREFEVVFLAALVGMEREEK KVIEHLRKNM GGALLML
Sbjct: 181 VELSRRMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLML 240

Query: 241 RSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 280
           RSAYGARAF+YPVVEACDLRGF+ILTVFHPTDEVINSIVLARL
Sbjct: 241 RSAYGARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 278

BLAST of Cmc05g0140511 vs. ExPASy TrEMBL
Match: A0A5A7TYM0 (Nicotianamine synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001120 PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.2e-123
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0

Query: 47  MASSSPSSIDVSSLCSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYY 106
           MASSSPSSIDVSSLCSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYY
Sbjct: 1   MASSSPSSIDVSSLCSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYY 60

Query: 107 SNYLKLTHLEFSILNHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSAN 166
           SNYLKLTHLEFSILNHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSAN
Sbjct: 61  SNYLKLTHLEFSILNHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSAN 120

Query: 167 AKASMLVAGDVELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRK 226
           AKASMLVAGDVELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRK
Sbjct: 121 AKASMLVAGDVELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRK 180

Query: 227 NMRGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 280
           NMRGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL
Sbjct: 181 NMRGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 233

BLAST of Cmc05g0140511 vs. ExPASy TrEMBL
Match: A0A6J1HS54 (nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 1.8e-119
Identity = 229/282 (81.21%), Postives = 251/282 (89.01%), Query Frame = 0

Query: 1   MCC--QSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVS 60
           MCC  + LLLVQK+SELYHKISTL+SL+PSKHVNSLFSQLVL    PP    SP  ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  SLCSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFS 120
            L S++QTMRS+LIKLCG+AE LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILNHHITPP-PSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDV 180
           IL  +I+P  PSKVAF+GSGALPFSSIIMALKYLP TEFHNFD+D SANAKA+MLVA DV
Sbjct: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180

Query: 181 ELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLR 240
           +LSRRM FHTTDIM+V S+ LREFEVVFLAALVGME+EEK KVIEHLRKNM  G+LLMLR
Sbjct: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 280
           SAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

BLAST of Cmc05g0140511 vs. ExPASy TrEMBL
Match: A0A6J1FHL8 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 8.3e-117
Identity = 226/282 (80.14%), Postives = 248/282 (87.94%), Query Frame = 0

Query: 1   MCC--QSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVS 60
           MCC  + LLLVQK+SELYHKISTL+SL+PSKHVNSLFSQLVL    PP    SP  ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  SLCSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFS 120
            L S++QTMRS+LIKLCG+AE LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILNHHITPP-PSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDV 180
           IL  +I+   PSKVAF+GSGALPFSSIIMALKYLP TEFHNFD+D SANAKA+ LVA DV
Sbjct: 121 ILTRYISSSLPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATALVASDV 180

Query: 181 ELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLR 240
           +LSRRM FHTTDIM+V S+ L EFEVVFLAALVGME+EEK KVIEHLRKNM  G+LLMLR
Sbjct: 181 DLSRRMSFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 280
           SAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

BLAST of Cmc05g0140511 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 313.5 bits (802), Expect = 1.7e-85
Identity = 160/281 (56.94%), Postives = 219/281 (77.94%), Query Frame = 0

Query: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60
           M CQ+ L+V++I +LY +IS L+SLKPSK+V++LF QLV   C P     + ++IDV+++
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLV-STCLP-----TDTNIDVTNM 60

Query: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSS---HQNPLDHLSLFPYYSNYLKLTHLEF 120
           C  ++ MR+NLIKLCGEAE  LE H+STIL S    QNPLDHL +FPYYSNYLKL  LEF
Sbjct: 61  CEEVKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEF 120

Query: 121 SILNHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDV 180
            +L+ H +  P+K+AFVGSG +P +SI++A  +LP T FHNFD+D  AN  AS LV+ D 
Sbjct: 121 DLLSQHSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDP 180

Query: 181 ELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLR 240
           +LS+RM+FHTTD++   +E L +++VVFLAALVGM++E K K IEHL K+M  GA+LMLR
Sbjct: 181 DLSKRMIFHTTDVLN-ATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 279
           SA+  RAFLYP+V++ DL+GF++LT++HPTD+V+NS+V+AR
Sbjct: 241 SAHALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

BLAST of Cmc05g0140511 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 312.0 bits (798), Expect = 4.9e-85
Identity = 166/278 (59.71%), Postives = 214/278 (76.98%), Query Frame = 0

Query: 3   CQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSLCS 62
           CQ   LV KI +LY KIS LE+LKP + V++LF QLV   C PP       +IDV+ +  
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLV-STCIPP-----NPNIDVTKMSE 63

Query: 63  SIQTMRSNLIKLCGEAESLLEHHYSTILSSHQ-NPLDHLSLFPYYSNYLKLTHLEFSILN 122
           +IQ MRSNLIK+CGEAE  LEHH+S+IL+S + NPL HL+LFPYY+NYLKL+ LEF +L 
Sbjct: 64  NIQEMRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLE 123

Query: 123 HHITP-PPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELS 182
            ++    P  VAF+GSG LP +S+++A  +L  + FHNFD+DPSAN  A+ LV+ D +LS
Sbjct: 124 QNLNGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLS 183

Query: 183 RRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAY 242
           +RM FHT DIM+V +E L+ F+VVFLAALVGM+++EK KV+EHL K+M  GALLMLRSA+
Sbjct: 184 QRMFFHTVDIMDV-TESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAH 243

Query: 243 GARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 279
           G RAFLYP+VE CDL GFE+L+V+HPTDEVINSIV++R
Sbjct: 244 GPRAFLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

BLAST of Cmc05g0140511 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 311.2 bits (796), Expect = 8.3e-85
Identity = 165/280 (58.93%), Postives = 216/280 (77.14%), Query Frame = 0

Query: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60
           M CQ   LVQ I +LY KIS LESLKPS+ VN LF QLV   C PP       +IDV+ +
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLV-STCIPP-----NPNIDVTKM 60

Query: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQ-NPLDHLSLFPYYSNYLKLTHLEFSI 120
           C  +Q +R NLIK+CG AE  LE+H+S+IL+S+Q NPL HL++FPYY+NYLKL  LEF +
Sbjct: 61  CDRVQEIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDL 120

Query: 121 LNHHITP-PPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVE 180
           L  ++    P  VAF+GSG LP +SI++A  +L  T FHNFD+DPSAN+ AS+LV+ D +
Sbjct: 121 LEQNLNGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPD 180

Query: 181 LSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRS 240
           +S+RM FHT DIM+V +E L+ F+VVFLAALVGM +EEK KVIEHL+K+M  GA+LMLRS
Sbjct: 181 ISQRMFFHTVDIMDV-TESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRS 240

Query: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 279
           A+G RAFLYP+VE CDL+GFE+L+++HPTD+VINS+V+++
Sbjct: 241 AHGPRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

BLAST of Cmc05g0140511 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 305.8 bits (782), Expect = 3.5e-83
Identity = 157/280 (56.07%), Postives = 218/280 (77.86%), Query Frame = 0

Query: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60
           M C++ L+V++I +LY++IS LESLKPSK+V++LF QLV   C P     + ++IDV+ +
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLV-STCLP-----TDTNIDVTEI 60

Query: 61  -CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQ-NPLDHLSLFPYYSNYLKLTHLEFS 120
               ++ MRS+LIKLCGEAE  LE H+S IL S + NPL+HL +FPYY+NYLKL  LEF 
Sbjct: 61  HDEKVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFD 120

Query: 121 ILNHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVE 180
           +L+ H T  P+KVAF+GSG +P +SI++A  +LP T FHNFD+D  AN  AS LV+ D +
Sbjct: 121 LLSQHTTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSD 180

Query: 181 LSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRS 240
           LS+RM+FHTTD++    E L +++VVFLAALVGM++E K K IEHL K+M  GA++MLRS
Sbjct: 181 LSKRMIFHTTDVLN-AKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRS 240

Query: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 279
           A+G RAFLYP+V++CDL+GFE+LT++HP+D+V+NS+V+AR
Sbjct: 241 AHGLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

BLAST of Cmc05g0140511 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 62.4 bits (150), Expect = 6.7e-10
Identity = 30/53 (56.60%), Postives = 41/53 (77.36%), Query Frame = 0

Query: 228 MRGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHP--TDEVINSIVLAR 279
           M  GALLMLRSA G R+FLY  V+ CDL+GFE+L ++HP  +D  +NS+++AR
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVAR 53

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008453494.12.6e-149100.00PREDICTED: nicotianamine synthase 1-like [Cucumis melo][more]
XP_004144812.16.7e-13792.58nicotianamine synthase 1 [Cucumis sativus][more]
XP_038891237.14.2e-13189.64probable nicotianamine synthase 4 [Benincasa hispida][more]
KAA0048068.12.5e-123100.00nicotianamine synthase 1-like [Cucumis melo var. makuwa] >TYJ96468.1 nicotianami... [more]
XP_022965879.13.7e-11981.21nicotianamine synthase [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FF792.3e-8456.94Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1[more]
Q9C7X56.8e-8459.71Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S... [more]
O804831.2e-8358.93Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1[more]
Q9XGI73.7e-8257.19Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1[more]
Q9FKT94.9e-8256.07Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BXJ61.3e-149100.00nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1[more]
A0A0A0LJ093.2e-13792.58Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1[more]
A0A5A7TYM01.2e-123100.00Nicotianamine synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A6J1HS541.8e-11981.21nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1[more]
A0A6J1FHL88.3e-11780.14nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G04950.11.7e-8556.94nicotianamine synthase 1 [more]
AT1G56430.14.9e-8559.71nicotianamine synthase 4 [more]
AT1G09240.18.3e-8558.93nicotianamine synthase 3 [more]
AT5G56080.13.5e-8356.07nicotianamine synthase 2 [more]
AT4G26483.16.7e-1056.60nicotianamine synthases [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004298Nicotianamine synthasePFAMPF03059NAScoord: 7..278
e-value: 3.0E-109
score: 364.6
IPR004298Nicotianamine synthasePANTHERPTHR32266NICOTIANAMINE SYNTHASE 3coord: 1..278
IPR004298Nicotianamine synthasePROSITEPS51142NAScoord: 1..279
score: 49.824211
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 2..279
e-value: 1.7E-61
score: 209.7
NoneNo IPR availablePANTHERPTHR32266:SF17SYNTHASE, PUTATIVE-RELATEDcoord: 1..278
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 90..239

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc05g0140511.1Cmc05g0140511.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030418 nicotianamine biosynthetic process
molecular_function GO:0030410 nicotianamine synthase activity