Cmc05g0129581 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc05g0129581
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	Reverse transcriptase
Location	CMiso1.1chr05: 7613502 .. 7613807 (-)
RNA-Seq Expression	Cmc05g0129581
Synteny	Cmc05g0129581

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTTTGGACTGTAGGGCCGACAACAATATCACTACAAAGTATCGTCATCCTATTTGTAGGCTAGATGGTATGTTTTATGAATTGCATGGTTCATGTTTCTCGACAAAGATTGATTTGAAATCTGGATATCATCAAGTTAGAATGCATGAAGGGGATGAATGGAAAATTGTCTTCAAAACTAAATTTGGATTGTATGAATGGTTTGTTATATCTTTTGTTTTAACTAATGCTCTTAGTACATTTATGCGTTTGATGAATCATTTTTTGGACAAATTTTTGTTGTTTACTTTGATGATACTTTGA

mRNA sequence

ATGTGTTTGGACTGTAGGGCCGACAACAATATCACTACAAAGTATCGTCATCCTATTTGTAGGCTAGATGGTATGTTTTATGAATTGCATGGTTCATGTTTCTCGACAAAGATTGATTTGAAATCTGGATATCATCAAGTTAGAATGCATGAAGGGGATGAATGGAAAATTGTCTTCAAAACTAAATTTGGATTGTATGAATGGTTTGTTATATCTTTTGTTTTAACTAATGCTCTTAGTACATTTATGCGTTTGATGAATCATTTTTTGGACAAATTTTTGTTGTTTACTTTGATGATACTTTGA

Coding sequence (CDS)

ATGTGTTTGGACTGTAGGGCCGACAACAATATCACTACAAAGTATCGTCATCCTATTTGTAGGCTAGATGGTATGTTTTATGAATTGCATGGTTCATGTTTCTCGACAAAGATTGATTTGAAATCTGGATATCATCAAGTTAGAATGCATGAAGGGGATGAATGGAAAATTGTCTTCAAAACTAAATTTGGATTGTATGAATGGTTTGTTATATCTTTTGTTTTAACTAATGCTCTTAGTACATTTATGCGTTTGATGAATCATTTTTTGGACAAATTTTTGTTGTTTACTTTGATGATACTTTGA

Protein sequence

MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFKTKFGLYEWFVISFVLTNALSTFMRLMNHFLDKFLLFTLMIL

Homology

[Close]

BLAST of Cmc05g0129581 vs. NCBI nr
Match: KAF5470845.1 (hypothetical protein F2P56_011333, partial [Juglans regia])

HSP 1 Score: 153.3 bits (386), Expect = 1.1e-33
Identity = 72/94 (76.60%), Postives = 77/94 (81.91%), Query Frame = 0


Query: 1   MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFK 60
           MC+DCRA NNIT KYRHPI RLD M  ELHGSC  TKIDLKSGYHQ+RM EGDEWK VFK
Sbjct: 553 MCVDCRAINNITVKYRHPIPRLDDMLDELHGSCVFTKIDLKSGYHQIRMKEGDEWKTVFK 612

Query: 61  TKFGLYEWFVISFVLTNALSTFMRLMNHFLDKFL 95
           TK+GLYEW V+ F LTNA STFMRLMNH L  F+
Sbjct: 613 TKYGLYEWLVMPFSLTNAPSTFMRLMNHVLRAFI 646

[Close]

BLAST of Cmc05g0129581 vs. NCBI nr
Match: CAN80356.1 (hypothetical protein VITISV_003506 [Vitis vinifera])

HSP 1 Score: 151.8 bits (382), Expect = 3.3e-33
Identity = 71/94 (75.53%), Postives = 76/94 (80.85%), Query Frame = 0


Query: 1   MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFK 60
           MC+DCRA NNIT KYRHPI RLD M  ELHGSC   KIDLKSGYHQ+RM EGDEWK  FK
Sbjct: 250 MCVDCRAINNITVKYRHPIPRLDDMLDELHGSCVFIKIDLKSGYHQIRMKEGDEWKTAFK 309

Query: 61  TKFGLYEWFVISFVLTNALSTFMRLMNHFLDKFL 95
           TK+GLYEW V+ F LTNALSTFMRLMNH L  F+
Sbjct: 310 TKYGLYEWLVMPFGLTNALSTFMRLMNHVLRAFI 343

[Close]

BLAST of Cmc05g0129581 vs. NCBI nr
Match: CAN73690.1 (hypothetical protein VITISV_034834 [Vitis vinifera])

HSP 1 Score: 151.4 bits (381), Expect = 4.3e-33
Identity = 71/94 (75.53%), Postives = 76/94 (80.85%), Query Frame = 0


Query: 1   MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFK 60
           MC+DCRA NNIT KYRHPI RLD M  ELHGSC  TKIDLKSGYHQ+RM EGDEWKI FK
Sbjct: 656 MCVDCRAINNITVKYRHPIPRLDDMLDELHGSCVFTKIDLKSGYHQIRMKEGDEWKIAFK 715

Query: 61  TKFGLYEWFVISFVLTNALSTFMRLMNHFLDKFL 95
           TK+GLYEW  + F LTNA STFMRLMNH L  F+
Sbjct: 716 TKYGLYEWLAMPFGLTNAPSTFMRLMNHALRAFI 749

[Close]

BLAST of Cmc05g0129581 vs. NCBI nr
Match: XP_030519546.1 (uncharacterized protein LOC115733005 [Rhodamnia argentea])

HSP 1 Score: 151.0 bits (380), Expect = 5.6e-33
Identity = 70/94 (74.47%), Postives = 76/94 (80.85%), Query Frame = 0


Query: 1   MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFK 60
           MC+DCRA NNIT KYRHPI RLD M  ELHG+C  TKIDLKSGYHQ+RM EGDEWK  FK
Sbjct: 488 MCIDCRAINNITVKYRHPIPRLDDMLDELHGACIFTKIDLKSGYHQIRMKEGDEWKTAFK 547

Query: 61  TKFGLYEWFVISFVLTNALSTFMRLMNHFLDKFL 95
           TK+GLYEW V+ F LTNA STFMRLMNH L  F+
Sbjct: 548 TKYGLYEWLVMPFGLTNAPSTFMRLMNHVLRAFI 581

[Close]

BLAST of Cmc05g0129581 vs. NCBI nr
Match: XP_035539637.1 (uncharacterized protein LOC109000794 [Juglans regia])

HSP 1 Score: 151.0 bits (380), Expect = 5.6e-33
Identity = 71/94 (75.53%), Postives = 76/94 (80.85%), Query Frame = 0


Query: 1   MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFK 60
           MC+DCRA NNIT KYRHPI RLD M  ELHGSC  TKIDLKSGYH +RM EGDEWK VFK
Sbjct: 624 MCVDCRAINNITVKYRHPIPRLDDMLDELHGSCVFTKIDLKSGYHHIRMKEGDEWKTVFK 683

Query: 61  TKFGLYEWFVISFVLTNALSTFMRLMNHFLDKFL 95
           TK+GLYEW V+ F LTNA STFMRLMNH L  F+
Sbjct: 684 TKYGLYEWLVMPFGLTNAPSTFMRLMNHVLRAFI 717

[Close]

BLAST of Cmc05g0129581 vs. ExPASy Swiss-Prot
Match: P31843 (RNA-directed DNA polymerase homolog OS=Oenothera berteroana OX=3950 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 2.9e-16
Identity = 44/94 (46.81%), Postives = 65/94 (69.15%), Query Frame = 0


Query: 1   MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFK 60
           MC+D RA   +T K ++PI R+D +F  L  + + TK+DL+SGY QVR+ +GDE K    
Sbjct: 8   MCIDYRALTKVTIKNKYPIPRVDDLFDRLAQATWFTKLDLRSGYWQVRIAKGDEPKTTCV 67

Query: 61  TKFGLYEWFVISFVLTNALSTFMRLMNHFLDKFL 95
           T++G +E+ V+ F LTNAL+TF  LMN+ L ++L
Sbjct: 68  TRYGSFEFRVMPFGLTNALATFCNLMNNVLYEYL 101

[Close]

BLAST of Cmc05g0129581 vs. ExPASy Swiss-Prot
Match: Q99315 (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 66.2 bits (160), Expect = 2.4e-10
Identity = 38/107 (35.51%), Postives = 55/107 (51.40%), Query Frame = 0


Query: 1   MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFK 60
           +C+D R  N  T     P+ R+D +   +  +   T +DL SGYHQ+ M   D +K  F 
Sbjct: 648 LCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIFTTLDLHSGYHQIPMEPKDRYKTAFV 707

Query: 61  TKFGLYEWFVISFVLTNALSTF----------MRLMNHFLDKFLLFT 98
           T  G YE+ V+ F L NA STF          +R +N +LD  L+F+
Sbjct: 708 TPSGKYEYTVMPFGLVNAPSTFARYMADTFRDLRFVNVYLDDILIFS 754

[Close]

BLAST of Cmc05g0129581 vs. ExPASy Swiss-Prot
Match: Q7LHG5 (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 2.4e-10
Identity = 38/107 (35.51%), Postives = 55/107 (51.40%), Query Frame = 0


Query: 1   MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFK 60
           +C+D R  N  T     P+ R+D +   +  +   T +DL SGYHQ+ M   D +K  F 
Sbjct: 674 LCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIFTTLDLHSGYHQIPMEPKDRYKTAFV 733

Query: 61  TKFGLYEWFVISFVLTNALSTF----------MRLMNHFLDKFLLFT 98
           T  G YE+ V+ F L NA STF          +R +N +LD  L+F+
Sbjct: 734 TPSGKYEYTVMPFGLVNAPSTFARYMADTFRDLRFVNVYLDDILIFS 780

[Close]

BLAST of Cmc05g0129581 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.0e-09
Identity = 37/92 (40.22%), Postives = 49/92 (53.26%), Query Frame = 0


Query: 3   LDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFKTK 62
           +D R  N IT   R+PI  +D +  +L    + T IDL  G+HQ+ M E    K  F TK
Sbjct: 265 IDYRKLNEITIPDRYPIPNMDEILGKLGKCQYFTTIDLAKGFHQIEMDEESISKTAFSTK 324

Query: 63  FGLYEWFVISFVLTNALSTFMRLMNHFLDKFL 95
            G YE+  + F L NA +TF R MN+ L   L
Sbjct: 325 SGHYEYLRMPFGLRNAPATFQRCMNNILRPLL 356

[Close]

BLAST of Cmc05g0129581 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.0e-09
Identity = 37/92 (40.22%), Postives = 47/92 (51.09%), Query Frame = 0


Query: 3   LDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFKTK 62
           +D R  N IT   RHPI  +D +  +L    + T IDL  G+HQ+ M      K  F TK
Sbjct: 266 IDYRKLNEITVGDRHPIPNMDEILGKLGRCNYFTTIDLAKGFHQIEMDPESVSKTAFSTK 325

Query: 63  FGLYEWFVISFVLTNALSTFMRLMNHFLDKFL 95
            G YE+  + F L NA +TF R MN  L   L
Sbjct: 326 HGHYEYLRMPFGLKNAPATFQRCMNDILRPLL 357

[Close]

BLAST of Cmc05g0129581 vs. ExPASy TrEMBL
Match: A0A2N9HEF3 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS38180 PE=4 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 6.4e-35
Identity = 73/94 (77.66%), Postives = 78/94 (82.98%), Query Frame = 0


Query: 1   MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFK 60
           MC+DCRA NNIT KYRHPI RLD M  ELHGSC  TKIDLKSGYHQ+RM EGDEWK  FK
Sbjct: 75  MCVDCRAINNITVKYRHPIPRLDDMLDELHGSCVFTKIDLKSGYHQIRMKEGDEWKTAFK 134

Query: 61  TKFGLYEWFVISFVLTNALSTFMRLMNHFLDKFL 95
           TK+GLYEWFV+ F LTNALSTFMRLMNH L  F+
Sbjct: 135 TKYGLYEWFVMPFGLTNALSTFMRLMNHVLRAFI 168

[Close]

BLAST of Cmc05g0129581 vs. ExPASy TrEMBL
Match: A0A2N9GU60 (Reverse transcriptase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS30900 PE=4 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 6.4e-35
Identity = 73/94 (77.66%), Postives = 78/94 (82.98%), Query Frame = 0


Query: 1   MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFK 60
           MC+DCRA NNIT KYRHPI RLD M  ELHGSC  TKIDLKSGYHQ+RM EGDEWK  FK
Sbjct: 306 MCVDCRAINNITVKYRHPIPRLDDMLDELHGSCVFTKIDLKSGYHQIRMKEGDEWKTAFK 365

Query: 61  TKFGLYEWFVISFVLTNALSTFMRLMNHFLDKFL 95
           TK+GLYEWFV+ F LTNALSTFMRLMNH L  F+
Sbjct: 366 TKYGLYEWFVMPFGLTNALSTFMRLMNHVLRAFI 399

[Close]

BLAST of Cmc05g0129581 vs. ExPASy TrEMBL
Match: A0A2N9H063 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS33075 PE=4 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 6.4e-35
Identity = 73/94 (77.66%), Postives = 78/94 (82.98%), Query Frame = 0


Query: 1   MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFK 60
           MC+DCRA NNIT KYRHPI RLD M  ELHGSC  TKIDLKSGYHQ+RM EGDEWK  FK
Sbjct: 595 MCVDCRAINNITVKYRHPIPRLDDMLDELHGSCVFTKIDLKSGYHQIRMKEGDEWKTAFK 654

Query: 61  TKFGLYEWFVISFVLTNALSTFMRLMNHFLDKFL 95
           TK+GLYEWFV+ F LTNALSTFMRLMNH L  F+
Sbjct: 655 TKYGLYEWFVMPFGLTNALSTFMRLMNHVLRAFI 688

[Close]

BLAST of Cmc05g0129581 vs. ExPASy TrEMBL
Match: A0A2N9J0J0 (Reverse transcriptase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS58968 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.4e-34
Identity = 73/94 (77.66%), Postives = 78/94 (82.98%), Query Frame = 0


Query: 1    MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFK 60
            MC+DCRA NNIT KYRHPI RLD M  ELHGSC  TKIDLKSGYHQ+RM EGDEWK VFK
Sbjct: 1587 MCVDCRAINNITVKYRHPIPRLDDMLDELHGSCVFTKIDLKSGYHQIRMKEGDEWKTVFK 1646

Query: 61   TKFGLYEWFVISFVLTNALSTFMRLMNHFLDKFL 95
            TK+GLYEWFV+ F LTNA STFMRLMNH L  F+
Sbjct: 1647 TKYGLYEWFVMPFGLTNAPSTFMRLMNHVLRAFI 1680

[Close]

BLAST of Cmc05g0129581 vs. ExPASy TrEMBL
Match: A0A2N9GCV0 (Reverse transcriptase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS28288 PE=4 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.4e-34
Identity = 72/94 (76.60%), Postives = 77/94 (81.91%), Query Frame = 0


Query: 1   MCLDCRADNNITTKYRHPICRLDGMFYELHGSCFSTKIDLKSGYHQVRMHEGDEWKIVFK 60
           MC+DCRA NNIT KY HPI RLD M  ELHGSC  TKIDLKSGYHQ+RM EGDEWK  FK
Sbjct: 283 MCVDCRAINNITVKYHHPIPRLDDMLDELHGSCVFTKIDLKSGYHQIRMKEGDEWKTAFK 342

Query: 61  TKFGLYEWFVISFVLTNALSTFMRLMNHFLDKFL 95
           TK+GLYEWFV+ F LTNALSTFMRLMNH L  F+
Sbjct: 343 TKYGLYEWFVMPFGLTNALSTFMRLMNHVLRAFI 376

The following BLAST results are available for this feature:

BLAST of Cmc05g0129581 vs. NCBI nr


Match Name	E-value	Identity	Description
KAF5470845.1	1.1e-33	76.60	hypothetical protein F2P56_011333, partial [Juglans regia]	[more]
CAN80356.1	3.3e-33	75.53	hypothetical protein VITISV_003506 [Vitis vinifera]	[more]
CAN73690.1	4.3e-33	75.53	hypothetical protein VITISV_034834 [Vitis vinifera]	[more]
XP_030519546.1	5.6e-33	74.47	uncharacterized protein LOC115733005 [Rhodamnia argentea]	[more]
XP_035539637.1	5.6e-33	75.53	uncharacterized protein LOC109000794 [Juglans regia]	[more]

BLAST of Cmc05g0129581 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P31843	2.9e-16	46.81	RNA-directed DNA polymerase homolog OS=Oenothera berteroana OX=3950 PE=4 SV=1	[more]
Q99315	2.4e-10	35.51	Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
Q7LHG5	2.4e-10	35.51	Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
P20825	2.0e-09	40.22	Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste...	[more]
P04323	2.0e-09	40.22	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast...	[more]

BLAST of Cmc05g0129581 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2N9HEF3	6.4e-35	77.66	Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F...	[more]
A0A2N9GU60	6.4e-35	77.66	Reverse transcriptase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS30900 PE=4 SV=1	[more]
A0A2N9H063	6.4e-35	77.66	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS33075 PE=4 SV=1	[more]
A0A2N9J0J0	1.4e-34	77.66	Reverse transcriptase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS58968 PE=4 SV=1	[more]
A0A2N9GCV0	2.4e-34	76.60	Reverse transcriptase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS28288 PE=4 SV=1	[more]

BLAST of Cmc05g0129581 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.10.10	HIV Type 1 Reverse Transcriptase, subunit A, domain 1	coord: 1..77 e-value: 2.3E-29 score: 104.2
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 1..95
None	No IPR available	PANTHER	PTHR24559:SF319	SUBFAMILY NOT NAMED	coord: 1..95
None	No IPR available	CDD	cd01647	RT_LTR	coord: 1..95 e-value: 1.45757E-32 score: 109.607
IPR043128	Reverse transcriptase/Diguanylate cyclase domain	GENE3D	3.30.70.270		coord: 17..91 e-value: 2.3E-29 score: 104.2
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 1..93

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc05g0129581.1	Cmc05g0129581.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
cellular_component	GO:0016020	membrane
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding