Cmc05g0125311 (gene) Melon (Charmono) v1.1

Overview
NameCmc05g0125311
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionGlucan endo-1,3-beta-D-glucosidase
LocationCMiso1.1chr05: 2396985 .. 2399608 (-)
RNA-Seq ExpressionCmc05g0125311
SyntenyCmc05g0125311
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGGCCCATCCATGATCTTCTCTCATCCCATTAATAAAAGACGCACCCATTTTTCTCTCCTCCTCCCACCCCCGCAGAACTCGACCCTCCACTCACTCTTTCTCTTTCTCATCCCCATATTCGTGCTTAAAACCCGTAACCAGAGATTTTGTTTTTATATCTCATTTCCTCTCACAACTCTGCTTCCTAATTAATGGAGCAACTCAAATCTCTCTGGTTTTTCTCCCTCTTCCTCCTTTTCCTCTCCCCATTTGCAGGTAGATTCATCCCTTTCCCTTTTACTGTTTCTCTATTACATTCTTCGCTCTCTTTTTCTTCTTCAATGATATGTGTTTTTCATTTTTGTCTTCTGTTTTGTTCCAGAGAGCGGTTCAATTGGAGTTAACTATGGCAGAATCGCCAACGACCTTCCTTCCGCCGTTCAAGTAGTCAAGCTTCTCAAATCCAATGGTCTTCAAAGGGTCAAAGTTTACGACACCGATCCCGCGGTTCTCAGAGCTCTTTCCGGCTCTGGGATTAAAGTCACCGTTGATTTGCCTAACGAACTTCTCTTCGCCGCTTCCCAACGTCTCTCCTTCGCTTACAATTGGGTCCAAAAGAATGTAGCCGCTTATTACCCTTCCACTGAAATCGAAGCCATTGCCGTCGGGAATGAAGTATTTGTCGACCCACATAACACCACGTCGTATCTTGTTCCGGCGATGAAGAACATTCATCAAGCTCTTGTCAAATACAACCTTGATTCCAAAATTATGGTCTCTTCTCCTATAGCTTTGAGCGCTTTACAAAACTCTTACCCTGCCTCAGCTGGTTCATTCCGGCCGGAGCTTGTCGAAACTGTTTTCCGTCCCATGTTGGATTTCCTCCGGCGAACTGGGTCTTATTTAATGGTTAACGCCTACCCATTTTTCGCTTACGAGTCCAACACCGATGTGATTTCTTTAGACTACGCTCTTTTCCGGGATAATCCCGGCGTCGTGGATACCGGCAGTGGTTACCGTTACTTCAGTTTATTCGACGCGCAAATCGACGCCGTGTTTGCCGCAATGTCGGCTCTGAAATACGACGACATTAATATGGTGGTTAGCGAAACAGGGTGGCCATCGAAAGGAGACGAAAATGAGATCGGAGCCAGTGTTGAAAACGCGGCCGCGTACAACGGAAACCTAGTCCATCGTATACTGAGCGGTGGCGGAACTCCGTTGAGACCAAAAGCAGATCTGACCGTCTATTTATTTGCTCTTTTCAACGAGAACAAAAAGAACGGTCCCACATCGGAAAGAAACTACGGCTTATTTTACCCAAACGAAGAGAAAGTTTACGACATCCCGTTTACTGCTGAAGGATTGAAAGAGTATAAGGACAAGCCATCGCGGAAGCCGATATCCGCGCCGACGGGAGCACCGGTGAGCGGCGGCGATGGTGGTGTCTCAAAGAGTCAAACGGGGAACACGTGGTGTGTTGCAAGTGGTGAAGCGGGAAGAGAGAAGCTGCAGGCAGGTCTAGACTATGCTTGTGGTGAAGGAGGGGCGGATTGCCGTCCGATCCAAGTGGGTGCCACGTGCTACAATCCGAATACGCTAGAGGCGCACGCTTCGTATGCTTTTAATAGTTATTACCAAAAGAACAACCGTAAGGTTGGCACGTGCTACTTTGGAGGGGCGGCTTACGTAGTCACTCAACCACCCAGTAAGTCCTCTCTTAAATTTCTTCTCTTTCTTTCTTTATTTTCATTTCTATAAATGGCGCACGTTAGAGAGGTTGGGATCCAACTCCCCCACTCGCGCCTCCGACTCGTGTTCTCTGCTAAAATGTTCCGGCCCGGACCACGAGGCTTAATAATGGAGCTGCACGCCAGCTCAGTAATAAGTCAAATTTCACTGCCCACATGCCGGCCACCTTTGAACTACGGAATCTCATCTTGCTTTCATGCTCTTTTTCTTTTCGTTTAATCCTCCATCCATTTACTCTGCGGTAAAAAGGTAAAGAAAAGGAAGAACATAGATTTTTCCCCATGTGATTTGAGGATAAACAAAGGACAAGTTGAAACAAAAGGCTTGGAATTGAAGTTTTGATTCTTCTGAAACCAAAACAAGCAGAAAAAGATGAATCTTTTACAACGGATGAATGCTTCTTTTTTCTTTTTTTTTCCCCCCCTCTTTTACTCAATGAGAGAAGACAAAAACTCAAGTGATTCTCAAACTTTTCTTGGTTTTTGTTTTGCTGTTTTGGTTAATTCTCATTTCCTTTGAGCAAGTTTCTGAAAATGGGTGTTTTCTTGTATGTATTGCAGAGTATGGCAGTTGTGAGTTCCCAACAGGGTATTGAAGAAGAGGAATGGGAATCTTAATGGGAGGGAGCAGTTGAAGGTTGAAGTGAGAAAAGGGGTTACTTTTTTCAACTTTGTTTTTAGAGGATAATTAATTCTTTTTGTAATCCAATTGCTTAGAATGATGGTGGTGGTTGGATTTTGGGGTTTTATTTTCGAAGCGTTTTCTGATTTGTAATTAACATGGTTTTCCTAATTGTTAGACTTCATCTACTTTTGTCATTTATCTAATTAATTGCTAGTAATTCTATATTTTAATACAAAGTTGTAATAACTCTATCTTTGCTTTAATTAT

mRNA sequence

TGGGCCCATCCATGATCTTCTCTCATCCCATTAATAAAAGACGCACCCATTTTTCTCTCCTCCTCCCACCCCCGCAGAACTCGACCCTCCACTCACTCTTTCTCTTTCTCATCCCCATATTCGTGCTTAAAACCCGTAACCAGAGATTTTGTTTTTATATCTCATTTCCTCTCACAACTCTGCTTCCTAATTAATGGAGCAACTCAAATCTCTCTGGTTTTTCTCCCTCTTCCTCCTTTTCCTCTCCCCATTTGCAGAGAGCGGTTCAATTGGAGTTAACTATGGCAGAATCGCCAACGACCTTCCTTCCGCCGTTCAAGTAGTCAAGCTTCTCAAATCCAATGGTCTTCAAAGGGTCAAAGTTTACGACACCGATCCCGCGGTTCTCAGAGCTCTTTCCGGCTCTGGGATTAAAGTCACCGTTGATTTGCCTAACGAACTTCTCTTCGCCGCTTCCCAACGTCTCTCCTTCGCTTACAATTGGGTCCAAAAGAATGTAGCCGCTTATTACCCTTCCACTGAAATCGAAGCCATTGCCGTCGGGAATGAAGTATTTGTCGACCCACATAACACCACGTCGTATCTTGTTCCGGCGATGAAGAACATTCATCAAGCTCTTGTCAAATACAACCTTGATTCCAAAATTATGGTCTCTTCTCCTATAGCTTTGAGCGCTTTACAAAACTCTTACCCTGCCTCAGCTGGTTCATTCCGGCCGGAGCTTGTCGAAACTGTTTTCCGTCCCATGTTGGATTTCCTCCGGCGAACTGGGTCTTATTTAATGGTTAACGCCTACCCATTTTTCGCTTACGAGTCCAACACCGATGTGATTTCTTTAGACTACGCTCTTTTCCGGGATAATCCCGGCGTCGTGGATACCGGCAGTGGTTACCGTTACTTCAGTTTATTCGACGCGCAAATCGACGCCGTGTTTGCCGCAATGTCGGCTCTGAAATACGACGACATTAATATGGTGGTTAGCGAAACAGGGTGGCCATCGAAAGGAGACGAAAATGAGATCGGAGCCAGTGTTGAAAACGCGGCCGCGTACAACGGAAACCTAGTCCATCGTATACTGAGCGGTGGCGGAACTCCGTTGAGACCAAAAGCAGATCTGACCGTCTATTTATTTGCTCTTTTCAACGAGAACAAAAAGAACGGTCCCACATCGGAAAGAAACTACGGCTTATTTTACCCAAACGAAGAGAAAGTTTACGACATCCCGTTTACTGCTGAAGGATTGAAAGAGTATAAGGACAAGCCATCGCGGAAGCCGATATCCGCGCCGACGGGAGCACCGGTGAGCGGCGGCGATGGTGGTGTCTCAAAGAGTCAAACGGGGAACACGTGGTGTGTTGCAAGTGGTGAAGCGGGAAGAGAGAAGCTGCAGGCAGGTCTAGACTATGCTTGTGGTGAAGGAGGGGCGGATTGCCGTCCGATCCAAGTGGGTGCCACGTGCTACAATCCGAATACGCTAGAGGCGCACGCTTCGTATGCTTTTAATAGTTATTACCAAAAGAACAACCGTAAGGTTGGCACGTGCTACTTTGGAGGGGCGGCTTACGTAGTCACTCAACCACCCAAGTATGGCAGTTGTGAGTTCCCAACAGGGTATTGAAGAAGAGGAATGGGAATCTTAATGGGAGGGAGCAGTTGAAGGTTGAAGTGAGAAAAGGGGTTACTTTTTTCAACTTTGTTTTTAGAGGATAATTAATTCTTTTTGTAATCCAATTGCTTAGAATGATGGTGGTGGTTGGATTTTGGGGTTTTATTTTCGAAGCGTTTTCTGATTTGTAATTAACATGGTTTTCCTAATTGTTAGACTTCATCTACTTTTGTCATTTATCTAATTAATTGCTAGTAATTCTATATTTTAATACAAAGTTGTAATAACTCTATCTTTGCTTTAATTAT

Coding sequence (CDS)

ATGGAGCAACTCAAATCTCTCTGGTTTTTCTCCCTCTTCCTCCTTTTCCTCTCCCCATTTGCAGAGAGCGGTTCAATTGGAGTTAACTATGGCAGAATCGCCAACGACCTTCCTTCCGCCGTTCAAGTAGTCAAGCTTCTCAAATCCAATGGTCTTCAAAGGGTCAAAGTTTACGACACCGATCCCGCGGTTCTCAGAGCTCTTTCCGGCTCTGGGATTAAAGTCACCGTTGATTTGCCTAACGAACTTCTCTTCGCCGCTTCCCAACGTCTCTCCTTCGCTTACAATTGGGTCCAAAAGAATGTAGCCGCTTATTACCCTTCCACTGAAATCGAAGCCATTGCCGTCGGGAATGAAGTATTTGTCGACCCACATAACACCACGTCGTATCTTGTTCCGGCGATGAAGAACATTCATCAAGCTCTTGTCAAATACAACCTTGATTCCAAAATTATGGTCTCTTCTCCTATAGCTTTGAGCGCTTTACAAAACTCTTACCCTGCCTCAGCTGGTTCATTCCGGCCGGAGCTTGTCGAAACTGTTTTCCGTCCCATGTTGGATTTCCTCCGGCGAACTGGGTCTTATTTAATGGTTAACGCCTACCCATTTTTCGCTTACGAGTCCAACACCGATGTGATTTCTTTAGACTACGCTCTTTTCCGGGATAATCCCGGCGTCGTGGATACCGGCAGTGGTTACCGTTACTTCAGTTTATTCGACGCGCAAATCGACGCCGTGTTTGCCGCAATGTCGGCTCTGAAATACGACGACATTAATATGGTGGTTAGCGAAACAGGGTGGCCATCGAAAGGAGACGAAAATGAGATCGGAGCCAGTGTTGAAAACGCGGCCGCGTACAACGGAAACCTAGTCCATCGTATACTGAGCGGTGGCGGAACTCCGTTGAGACCAAAAGCAGATCTGACCGTCTATTTATTTGCTCTTTTCAACGAGAACAAAAAGAACGGTCCCACATCGGAAAGAAACTACGGCTTATTTTACCCAAACGAAGAGAAAGTTTACGACATCCCGTTTACTGCTGAAGGATTGAAAGAGTATAAGGACAAGCCATCGCGGAAGCCGATATCCGCGCCGACGGGAGCACCGGTGAGCGGCGGCGATGGTGGTGTCTCAAAGAGTCAAACGGGGAACACGTGGTGTGTTGCAAGTGGTGAAGCGGGAAGAGAGAAGCTGCAGGCAGGTCTAGACTATGCTTGTGGTGAAGGAGGGGCGGATTGCCGTCCGATCCAAGTGGGTGCCACGTGCTACAATCCGAATACGCTAGAGGCGCACGCTTCGTATGCTTTTAATAGTTATTACCAAAAGAACAACCGTAAGGTTGGCACGTGCTACTTTGGAGGGGCGGCTTACGTAGTCACTCAACCACCCAAGTATGGCAGTTGTGAGTTCCCAACAGGGTATTGA

Protein sequence

MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDTDPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVETVFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFDAQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRKPISAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGATCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY
Homology
BLAST of Cmc05g0125311 vs. NCBI nr
Match: XP_008449478.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 12 [Cucumis melo] >KAA0061649.1 glucan endo-1,3-beta-glucosidase 12 [Cucumis melo var. makuwa] >TYK05857.1 glucan endo-1,3-beta-glucosidase 12 [Cucumis melo var. makuwa])

HSP 1 Score: 946.8 bits (2446), Expect = 7.1e-272
Identity = 472/474 (99.58%), Postives = 472/474 (99.58%), Query Frame = 0

Query: 1   MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT 60
           MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT
Sbjct: 1   MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT 60

Query: 61  DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV 120
           DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV
Sbjct: 61  DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV 120

Query: 121 FVDPHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVET 180
           FVDPHNTTSYLVPAMKNIHQALVK NLDSKI VSSPIALSALQNSYPASAGSFRPELVET
Sbjct: 121 FVDPHNTTSYLVPAMKNIHQALVKNNLDSKIKVSSPIALSALQNSYPASAGSFRPELVET 180

Query: 181 VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD 240
           VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD
Sbjct: 181 VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD 240

Query: 241 AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT 300
           AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT
Sbjct: 241 AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT 300

Query: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK 360
           PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK
Sbjct: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK 360

Query: 361 PISAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGA 420
           PISAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGA
Sbjct: 361 PISAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGA 420

Query: 421 TCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 475
           TCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY
Sbjct: 421 TCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 474

BLAST of Cmc05g0125311 vs. NCBI nr
Match: XP_004140121.1 (glucan endo-1,3-beta-glucosidase 12 [Cucumis sativus] >KGN47963.1 hypothetical protein Csa_003377 [Cucumis sativus])

HSP 1 Score: 882.1 bits (2278), Expect = 2.1e-252
Identity = 438/477 (91.82%), Postives = 459/477 (96.23%), Query Frame = 0

Query: 1   MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT 60
           MEQLKSLWFF  FLLFLSPFAESGSIGVNYGRI NDLPSAV+VVKLLKS+GLQRVKVYDT
Sbjct: 1   MEQLKSLWFFYFFLLFLSPFAESGSIGVNYGRIGNDLPSAVKVVKLLKSHGLQRVKVYDT 60

Query: 61  DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV 120
           DPAVL+ALSGSGIKVTVDLPNELLFAA++RL+FAY WV+KNVAAYYPSTEIEAIAVGNEV
Sbjct: 61  DPAVLKALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEV 120

Query: 121 FVDPHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVET 180
           FVDPHNTTS+LVPAMKNIHQALVKYNL S I VSSPIALSALQNSYP+SAGSFRPELVET
Sbjct: 121 FVDPHNTTSFLVPAMKNIHQALVKYNLHSNIKVSSPIALSALQNSYPSSAGSFRPELVET 180

Query: 181 VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD 240
           VFRPML+FLR+TGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVD GSGYRYF+LFD
Sbjct: 181 VFRPMLEFLRQTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDAGSGYRYFNLFD 240

Query: 241 AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT 300
           AQIDAVFAAMSALKYDDI MVV+ETGWPSKGDENEIGASVENAAAYNGNLV RILSGGGT
Sbjct: 241 AQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGT 300

Query: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK 360
           PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFT EGLK+++DKPS K
Sbjct: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTTEGLKDFEDKPSPK 360

Query: 361 PIS---APTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQ 420
           P+S   APT AP + GDGGVSKSQTGNTWCVASGEAG+EKLQ+GLDYACGEGGADCRPIQ
Sbjct: 361 PVSGGNAPT-APPASGDGGVSKSQTGNTWCVASGEAGKEKLQSGLDYACGEGGADCRPIQ 420

Query: 421 VGATCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 475
           VGATCYNPNTLEAHASYAFNSYYQKN+RKVGTCYFGGAAYVVTQPPKYGSCEFPTGY
Sbjct: 421 VGATCYNPNTLEAHASYAFNSYYQKNSRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 476

BLAST of Cmc05g0125311 vs. NCBI nr
Match: XP_038886813.1 (LOW QUALITY PROTEIN: glucan endo-1,3-beta-glucosidase 12 [Benincasa hispida])

HSP 1 Score: 838.6 bits (2165), Expect = 2.7e-239
Identity = 419/476 (88.03%), Postives = 443/476 (93.07%), Query Frame = 0

Query: 6   SLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDTDPAVL 65
           +L FF +F LFLS FAESGSIGVNYGRIAN+LPSAV+VVKLLK++GLQRVKVYDTDPAVL
Sbjct: 5   TLCFFFIFPLFLSSFAESGSIGVNYGRIANNLPSAVKVVKLLKTHGLQRVKVYDTDPAVL 64

Query: 66  RALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEVFVDPH 125
           RALSGSGIKVTVDLPNELLFAA++RL+FAY WV+KNVAAYYPSTEIEAIAVGNEVFVDPH
Sbjct: 65  RALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPH 124

Query: 126 NTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVETVFRPM 185
           NTTS+LVPAMKNIHQALVKYNL S I VSSPIALSALQNSYP+SAGSFRPELVETVFRPM
Sbjct: 125 NTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPM 184

Query: 186 LDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFDAQIDA 245
           L+FLR+TGSYLMVNAYPFFAYESN DVISLDYALFRDNPGVVD GSGYRYF+LFDAQIDA
Sbjct: 185 LEFLRQTGSYLMVNAYPFFAYESNADVISLDYALFRDNPGVVDAGSGYRYFNLFDAQIDA 244

Query: 246 VFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGTPLRPK 305
           VFAAMSALKYDDI MVV+ETGWPSKGDENEIGASVENAAAYNGNLV RIL+GGGTPLRPK
Sbjct: 245 VFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILNGGGTPLRPK 304

Query: 306 ADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRKPISAP 365
           ADLTVYLFALFNENKKNGP SERNYGLFYPNEEKVY+IPFT EGLK+Y DKPS  P++  
Sbjct: 305 ADLTVYLFALFNENKKNGPASERNYGLFYPNEEKVYEIPFTTEGLKDYHDKPSPAPVTGG 364

Query: 366 TG--APVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGG-----ADCRPIQV 425
            G   P   GDGGVSKSQTGNTWCVASGEAG+EKLQ+ LDYACGEGG     ADCRPIQ 
Sbjct: 365 NGPSGPPESGDGGVSKSQTGNTWCVASGEAGKEKLQSSLDYACGEGGXXXXXADCRPIQP 424

Query: 426 GATCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 475
           GATCYNPNTLEAHASYAFNSYYQKN+RKVGTCYFGGAAYVVTQPPKYGSCEFPTGY
Sbjct: 425 GATCYNPNTLEAHASYAFNSYYQKNSRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 480

BLAST of Cmc05g0125311 vs. NCBI nr
Match: XP_022952844.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata])

HSP 1 Score: 838.6 bits (2165), Expect = 2.7e-239
Identity = 421/475 (88.63%), Postives = 448/475 (94.32%), Query Frame = 0

Query: 1   MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT 60
           ME+L +L FF +FLLFLSPFAESGSIGVNYGRIANDLPSAV+VVKLLKS+GLQRVKVYDT
Sbjct: 1   MERL-TLSFF-IFLLFLSPFAESGSIGVNYGRIANDLPSAVKVVKLLKSHGLQRVKVYDT 60

Query: 61  DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV 120
           DPAVLRALSGSGIKVTVDLPNELLFAA++RLSFAY WV+KNVAAYYPSTEIEAIAVGNEV
Sbjct: 61  DPAVLRALSGSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEV 120

Query: 121 FVDPHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVET 180
           FVDPHNTTS+LVPAMKNIHQALVK+NL S I VSSPIALSALQNSYP+SAGSFRPELVE 
Sbjct: 121 FVDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEP 180

Query: 181 VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD 240
           VFRPML+FLR+TGSYLMVNAYPFFAYESN+DVISLDYALFR+NPGVVD+GSGYRYF+LFD
Sbjct: 181 VFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFD 240

Query: 241 AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT 300
           AQIDAVFAAMSALKYDDI +VV+ETGWPSKGDENEIGASV NAAAYNGNL+ RILSGGGT
Sbjct: 241 AQIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGT 300

Query: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK 360
           PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE+VYDIPFT E LK+Y DKPS  
Sbjct: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPA 360

Query: 361 PISAPTGAPVSG-GDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVG 420
           P +   G   SG GDGGVSKSQTGNTWCVASGEAG+EKLQAGLDYACGEGGADCRPIQ  
Sbjct: 361 PATGGDGQAGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 475
           ATCYNPNTL+AHASYAFNSYYQKN RK+GTCYFGGAAYVVTQPPK+GSCEFPTGY
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPPKFGSCEFPTGY 473

BLAST of Cmc05g0125311 vs. NCBI nr
Match: KAG6572415.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012014.1 Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 834.7 bits (2155), Expect = 3.9e-238
Identity = 418/475 (88.00%), Postives = 448/475 (94.32%), Query Frame = 0

Query: 1   MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT 60
           ME+L +L FF +F+LFLSPFAESGSIGVNYGRIAN+LPSAV+VVKLLK++GLQRVKVYDT
Sbjct: 1   MERL-TLSFF-IFILFLSPFAESGSIGVNYGRIANNLPSAVKVVKLLKTHGLQRVKVYDT 60

Query: 61  DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV 120
           DPAVLRALSGSGIKVTVDLPNELLFAA++RL+FAY WV+KNVAAYYPSTEIEAIAVGNEV
Sbjct: 61  DPAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEV 120

Query: 121 FVDPHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVET 180
           FVDPHNTTS+LVPAMKNIHQALVK+NL S I VSSPIALSALQNSYP+SAGSFRPELVE 
Sbjct: 121 FVDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEP 180

Query: 181 VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD 240
           VFRPML+FLR+TGSYLMVNAYPFFAYESN+DVISLDYALFR+NPGVVD+GSGYRYF+LFD
Sbjct: 181 VFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFD 240

Query: 241 AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT 300
           AQIDAVFAAMSALKYDDI MVV+ETGWPSKGDENEIGASV NAAAYNGNL+ RILSGGGT
Sbjct: 241 AQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGT 300

Query: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK 360
           PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE+VYDIPFT E LK+Y DKPS  
Sbjct: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPA 360

Query: 361 PISAPTGAPVSG-GDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVG 420
           P +   G   SG GDGGVSKSQTGNTWCVASGEAG+EKLQAGLDYACGEGGADCRPIQ  
Sbjct: 361 PATGGDGQTGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 475
           ATCYNPNTL+AHASYAFNSYYQKN RK+GTCYFGGAAYVVTQPPK+GSCEFPTGY
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPPKFGSCEFPTGY 473

BLAST of Cmc05g0125311 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.5e-99
Identity = 190/466 (40.77%), Postives = 285/466 (61.16%), Query Frame = 0

Query: 8   WFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDTDPAVLRA 67
           W F   L FL+ F  +  IG+ YGR A++LPS  +V +L++   ++ V++YD +  VL+A
Sbjct: 10  WIFVSILAFLN-FGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKA 69

Query: 68  LSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEVFVDPHNT 127
            + +GI++ + +PN  L A +Q  S    W+  N+  YYPST+I +I+VG EV   P N 
Sbjct: 70  FANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNA 129

Query: 128 TSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVETVFRPMLD 187
           T  ++PAM+NIH AL K  LD KI +SS  +L+ L  S+P S+ SF  +      +PML+
Sbjct: 130 TGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HSAFLKPMLE 189

Query: 188 FLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFDAQIDAVF 247
           FL    S  M++ YP++AY  +T+ + L+YALF  +  VVD  +G  Y ++FDAQ+DA++
Sbjct: 190 FLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIY 249

Query: 248 AAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGTPLRPKAD 307
            A++A+ +  + ++V+E+GWPSKG   E  A+ ENA AYN NL+  ++   GTP +P  +
Sbjct: 250 FALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEE 309

Query: 308 LTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRKPIS-APT 367
           + VYLF+LFNEN+K G  SERN+G+FY N   VY + FT E         S    S +P+
Sbjct: 310 IDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPS 369

Query: 368 GAPVSGGDGGVSKSQTG----NTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGATC 427
            +P+  G+  V+    G      WC+AS +A   +LQ  LD+ACG G  DC  +Q    C
Sbjct: 370 SSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPC 429

Query: 428 YNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSC 469
           + P+T+ +HASYAFN+YYQ++      C F GA+  V + P YG+C
Sbjct: 430 FEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNC 473

BLAST of Cmc05g0125311 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 5.9e-96
Identity = 185/468 (39.53%), Postives = 276/468 (58.97%), Query Frame = 0

Query: 7   LWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDTDPAVLR 66
           L F    LL L      G +GV YGR A+DLP+  +VV+L++ + ++ V++YD +  VL+
Sbjct: 7   LIFSISILLLLLDCCYGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLK 66

Query: 67  ALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEVFVDPH- 126
           A   + I++ + +PN  L A SQ  S    W++ +V  YYP+T+I  I VG E   DPH 
Sbjct: 67  AFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHI 126

Query: 127 NTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVETVFRPM 186
           N +S++VPAM+N+  AL K  L  +I VS+ ++L  L  S+P SAG+F         RPM
Sbjct: 127 NASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYA-YFLRPM 186

Query: 187 LDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFDAQIDA 246
           L+FL    S  M++ YP++AY  + + +SLDY LF  +  V+D  +G  Y ++FDAQ+DA
Sbjct: 187 LEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDA 246

Query: 247 VFAAMSALKYDDINMVVSETGWPSKGDENE-IGASVENAAAYNGNLVHRILSGGGTPLRP 306
           ++ A++AL +  I ++V+ETGWP+KG   E   AS +NA  YN N++  +++  GTP +P
Sbjct: 247 LYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKP 306

Query: 307 KADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRKPISA 366
              + VY+F+LFNEN+K G  SERN+GLFYP++  VY + FT +    + +         
Sbjct: 307 GEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSN--------- 366

Query: 367 PTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGATCYN 426
                      G + S + N+WC+AS +A    L+  LD+ACG G  DC  IQ    C+ 
Sbjct: 367 ---------SSGTNSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQ 426

Query: 427 PNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPT 473
           P+TL +HAS+ FNSY+Q+N      C FGGA   V + P Y  C + T
Sbjct: 427 PDTLVSHASFVFNSYFQQNRATDVACSFGGAGVKVNKDPSYDKCIYIT 455

BLAST of Cmc05g0125311 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 351.3 bits (900), Expect = 1.7e-95
Identity = 192/471 (40.76%), Postives = 275/471 (58.39%), Query Frame = 0

Query: 4   LKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDTDPA 63
           + +L    LFL   S  ++   IGVN G    ++PS  QVV LLKS  + RV++YD D +
Sbjct: 1   MAALLLLFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRS 60

Query: 64  VLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEVFVD 123
           +L A + +G++V + +PN+ L   SQ  + A NWV +NVAAYYP+T I  IAVG+EV   
Sbjct: 61  MLLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTS 120

Query: 124 PHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVETVFR 183
             N  S LV A+K I  ALV  NLD +I VS+P + + + +S+P S   F  +  + V  
Sbjct: 121 LTNAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFN-KTWDPVIV 180

Query: 184 PMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRD---NPGVVDTGSGYRYFSLFD 243
           P+L FL+ TGS L++N YP+F Y  +  VI LDYALF+    N   VD  +   Y ++FD
Sbjct: 181 PLLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFD 240

Query: 244 AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT 303
           A +DA + AMS L + +I +VV+E+GWPSKG  +E  A+VENA  YN NL+  +++  GT
Sbjct: 241 AIVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGT 300

Query: 304 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK 363
           P  P   +T Y++ L+NE+ + GP SE+N+GLFY N   VY +                 
Sbjct: 301 PKHPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTL----------------- 360

Query: 364 PISAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGA 423
                    ++G    ++   T  T+C+A  +  R+ LQA LD+ACG G  DC  +  G 
Sbjct: 361 --------RLAGAGAILANDTTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGE 420

Query: 424 TCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFP 472
           +CY P+ + AH++YAFN+YYQK  +  G+C F G A V T  P  G+C FP
Sbjct: 421 SCYEPDDVVAHSTYAFNAYYQKMGKASGSCDFKGVATVTTTDPSRGTCVFP 445

BLAST of Cmc05g0125311 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 348.6 bits (893), Expect = 1.1e-94
Identity = 196/479 (40.92%), Postives = 275/479 (57.41%), Query Frame = 0

Query: 1   MEQLKSLWFFSLFLLFL-----SPFAESGS-IGVNYGRIANDLPSAVQVVKLLKSNGLQR 60
           M  L  L   SL LL L     SP A+ GS IGVN G   +D+P   QVV LLK+  ++ 
Sbjct: 1   MASLLHLLLLSLSLLVLASASPSPPADEGSYIGVNIGTDLSDMPHPTQVVALLKAQEIRH 60

Query: 61  VKVYDTDPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAI 120
           +++Y+ DP +L AL+ +GIKV + +PN+ L    Q  S A NWV++NV A+YP+T I A+
Sbjct: 61  IRLYNADPGLLIALANTGIKVIISIPNDQLLGIGQSNSTAANWVKRNVIAHYPATMITAV 120

Query: 121 AVGNEVFVDPHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFR 180
           +VG+EV     N    LV A+KN+H AL+  NLD  I VS+P++ S + + +P S   F 
Sbjct: 121 SVGSEVLTSLSNAAPVLVSAIKNVHAALLSANLDKLIKVSTPLSTSLILDPFPPSQAFFN 180

Query: 181 PELVETVFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFR---DNPGVVDTGS 240
             L   V  P+L FL+ T SYLMVN YP+  Y  +  VI LDYALF+    N   VD  +
Sbjct: 181 RSL-NAVIVPLLSFLQSTNSYLMVNVYPYIDYMQSNGVIPLDYALFKPIPPNKEAVDANT 240

Query: 241 GYRYFSLFDAQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLV 300
             RY + FDA +DA + AM+ L + +I ++V+E+GWPSKG+ NE  A+++NA  YN NL+
Sbjct: 241 LVRYSNAFDAMVDATYFAMAFLNFTNIPVLVTESGWPSKGETNEPDATLDNANTYNSNLI 300

Query: 301 HRILSGGGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLK 360
             +L+  GTP RP   ++ Y++ L+NE+ K G  SE+N+GLF  N E VY +  T  G  
Sbjct: 301 RHVLNKTGTPKRPGIAVSTYIYELYNEDTKAG-LSEKNWGLFNANGEPVYVLRLTNSG-- 360

Query: 361 EYKDKPSRKPISAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGA 420
                                    ++   T  T+C A   A  + LQA LD+ACG G  
Sbjct: 361 -----------------------SVLANDTTNQTYCTAREGADTKMLQAALDWACGPGKI 420

Query: 421 DCRPIQVGATCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEF 471
           DC PI+ G TCY P+ + AHA+YAF++YY +       C F G A + T  P +G+C F
Sbjct: 421 DCSPIKQGETCYEPDNVVAHANYAFDTYYHQTGNNPDACNFNGVASITTTDPSHGTCVF 452

BLAST of Cmc05g0125311 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 347.1 bits (889), Expect = 3.3e-94
Identity = 191/465 (41.08%), Postives = 285/465 (61.29%), Query Frame = 0

Query: 14  LLFLSPFAESGS---IGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDTDPAVLRALSG 73
           L+FLS F  S +   IGVNYG++A++LP   + VKLL+S  +Q+V++Y  DPA+++AL+G
Sbjct: 11  LIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAG 70

Query: 74  SGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEVFV--DPHNTT 133
           +G+ + +   N  + + +   + A  W+  NV  +YP+++I  I VGNE+ +  DP N  
Sbjct: 71  TGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDP-NLV 130

Query: 134 SYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVETVFRPMLDF 193
           + L+PAM+N+ +AL   +L  KI VS+  +++ L +S P S+GSF     +T  + +L F
Sbjct: 131 NQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAG-YQTGLKGILQF 190

Query: 194 LRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFDAQIDAVFA 253
           L  TGS   +N YPFFAY+S+    +L + LF  N G VD+ +G +Y ++FDAQ+DAV +
Sbjct: 191 LSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHS 250

Query: 254 AMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGTPLRPKADL 313
           A+ ++ ++ + +VV+ETGW S+GD NE+GASV+NA AYNGNL+  + S  GTPL P   +
Sbjct: 251 ALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPV 310

Query: 314 TVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRKPISAPTGA 373
             Y+FAL++EN K GP+SER +GLF  +   VYD+     GL +     S      P+G 
Sbjct: 311 DTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDV-----GLAK-----SSSSSQTPSGK 370

Query: 374 PVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGATCYNPNTL 433
             S G            WCV    A  E+LQA LD+ACG  G DC  IQ G  C+ PN +
Sbjct: 371 VTSSG------------WCVPKKGATNEELQASLDWACGH-GIDCGAIQPGGACFEPNNV 430

Query: 434 EAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTG 474
            +HA+YA N Y+QK+ ++   C F   A V +Q P Y +C +P G
Sbjct: 431 VSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGG 450

BLAST of Cmc05g0125311 vs. ExPASy TrEMBL
Match: A0A5D3C206 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1465G00120 PE=3 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 3.4e-272
Identity = 472/474 (99.58%), Postives = 472/474 (99.58%), Query Frame = 0

Query: 1   MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT 60
           MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT
Sbjct: 1   MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT 60

Query: 61  DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV 120
           DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV
Sbjct: 61  DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV 120

Query: 121 FVDPHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVET 180
           FVDPHNTTSYLVPAMKNIHQALVK NLDSKI VSSPIALSALQNSYPASAGSFRPELVET
Sbjct: 121 FVDPHNTTSYLVPAMKNIHQALVKNNLDSKIKVSSPIALSALQNSYPASAGSFRPELVET 180

Query: 181 VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD 240
           VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD
Sbjct: 181 VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD 240

Query: 241 AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT 300
           AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT
Sbjct: 241 AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT 300

Query: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK 360
           PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK
Sbjct: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK 360

Query: 361 PISAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGA 420
           PISAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGA
Sbjct: 361 PISAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGA 420

Query: 421 TCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 475
           TCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY
Sbjct: 421 TCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 474

BLAST of Cmc05g0125311 vs. ExPASy TrEMBL
Match: A0A1S3BMR4 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103491351 PE=3 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 3.4e-272
Identity = 472/474 (99.58%), Postives = 472/474 (99.58%), Query Frame = 0

Query: 1   MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT 60
           MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT
Sbjct: 1   MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT 60

Query: 61  DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV 120
           DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV
Sbjct: 61  DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV 120

Query: 121 FVDPHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVET 180
           FVDPHNTTSYLVPAMKNIHQALVK NLDSKI VSSPIALSALQNSYPASAGSFRPELVET
Sbjct: 121 FVDPHNTTSYLVPAMKNIHQALVKNNLDSKIKVSSPIALSALQNSYPASAGSFRPELVET 180

Query: 181 VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD 240
           VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD
Sbjct: 181 VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD 240

Query: 241 AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT 300
           AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT
Sbjct: 241 AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT 300

Query: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK 360
           PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK
Sbjct: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK 360

Query: 361 PISAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGA 420
           PISAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGA
Sbjct: 361 PISAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGA 420

Query: 421 TCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 475
           TCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY
Sbjct: 421 TCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 474

BLAST of Cmc05g0125311 vs. ExPASy TrEMBL
Match: A0A0A0KEL0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G421570 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.0e-252
Identity = 438/477 (91.82%), Postives = 459/477 (96.23%), Query Frame = 0

Query: 1   MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT 60
           MEQLKSLWFF  FLLFLSPFAESGSIGVNYGRI NDLPSAV+VVKLLKS+GLQRVKVYDT
Sbjct: 1   MEQLKSLWFFYFFLLFLSPFAESGSIGVNYGRIGNDLPSAVKVVKLLKSHGLQRVKVYDT 60

Query: 61  DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV 120
           DPAVL+ALSGSGIKVTVDLPNELLFAA++RL+FAY WV+KNVAAYYPSTEIEAIAVGNEV
Sbjct: 61  DPAVLKALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEV 120

Query: 121 FVDPHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVET 180
           FVDPHNTTS+LVPAMKNIHQALVKYNL S I VSSPIALSALQNSYP+SAGSFRPELVET
Sbjct: 121 FVDPHNTTSFLVPAMKNIHQALVKYNLHSNIKVSSPIALSALQNSYPSSAGSFRPELVET 180

Query: 181 VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD 240
           VFRPML+FLR+TGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVD GSGYRYF+LFD
Sbjct: 181 VFRPMLEFLRQTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDAGSGYRYFNLFD 240

Query: 241 AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT 300
           AQIDAVFAAMSALKYDDI MVV+ETGWPSKGDENEIGASVENAAAYNGNLV RILSGGGT
Sbjct: 241 AQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGT 300

Query: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK 360
           PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFT EGLK+++DKPS K
Sbjct: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTTEGLKDFEDKPSPK 360

Query: 361 PIS---APTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQ 420
           P+S   APT AP + GDGGVSKSQTGNTWCVASGEAG+EKLQ+GLDYACGEGGADCRPIQ
Sbjct: 361 PVSGGNAPT-APPASGDGGVSKSQTGNTWCVASGEAGKEKLQSGLDYACGEGGADCRPIQ 420

Query: 421 VGATCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 475
           VGATCYNPNTLEAHASYAFNSYYQKN+RKVGTCYFGGAAYVVTQPPKYGSCEFPTGY
Sbjct: 421 VGATCYNPNTLEAHASYAFNSYYQKNSRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 476

BLAST of Cmc05g0125311 vs. ExPASy TrEMBL
Match: A0A6J1GMW6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111455410 PE=3 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.3e-239
Identity = 421/475 (88.63%), Postives = 448/475 (94.32%), Query Frame = 0

Query: 1   MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT 60
           ME+L +L FF +FLLFLSPFAESGSIGVNYGRIANDLPSAV+VVKLLKS+GLQRVKVYDT
Sbjct: 1   MERL-TLSFF-IFLLFLSPFAESGSIGVNYGRIANDLPSAVKVVKLLKSHGLQRVKVYDT 60

Query: 61  DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV 120
           DPAVLRALSGSGIKVTVDLPNELLFAA++RLSFAY WV+KNVAAYYPSTEIEAIAVGNEV
Sbjct: 61  DPAVLRALSGSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEV 120

Query: 121 FVDPHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVET 180
           FVDPHNTTS+LVPAMKNIHQALVK+NL S I VSSPIALSALQNSYP+SAGSFRPELVE 
Sbjct: 121 FVDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEP 180

Query: 181 VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD 240
           VFRPML+FLR+TGSYLMVNAYPFFAYESN+DVISLDYALFR+NPGVVD+GSGYRYF+LFD
Sbjct: 181 VFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFD 240

Query: 241 AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT 300
           AQIDAVFAAMSALKYDDI +VV+ETGWPSKGDENEIGASV NAAAYNGNL+ RILSGGGT
Sbjct: 241 AQIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGT 300

Query: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK 360
           PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE+VYDIPFT E LK+Y DKPS  
Sbjct: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPA 360

Query: 361 PISAPTGAPVSG-GDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVG 420
           P +   G   SG GDGGVSKSQTGNTWCVASGEAG+EKLQAGLDYACGEGGADCRPIQ  
Sbjct: 361 PATGGDGQAGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 475
           ATCYNPNTL+AHASYAFNSYYQKN RK+GTCYFGGAAYVVTQPPK+GSCEFPTGY
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPPKFGSCEFPTGY 473

BLAST of Cmc05g0125311 vs. ExPASy TrEMBL
Match: A0A6J1I0V3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111468418 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 4.2e-238
Identity = 419/475 (88.21%), Postives = 446/475 (93.89%), Query Frame = 0

Query: 1   MEQLKSLWFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDT 60
           ME+L +L FF +FLLFL PFAESGSIGVNYGRIAN+LPSAV+VVKLLKS+GLQRVKVYDT
Sbjct: 1   MERL-TLSFF-IFLLFLYPFAESGSIGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDT 60

Query: 61  DPAVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEV 120
           DPAVLRALSGSGIKVTVDLPNELLFAA++RLSFAY WV+KNVAAYYPSTEIEAIAVGNEV
Sbjct: 61  DPAVLRALSGSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEV 120

Query: 121 FVDPHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVET 180
           FVDPHNTTS+LVPAMKNIHQALVK+NL S I VSSPIALSALQNSYP+SAGSFRPELVE 
Sbjct: 121 FVDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEP 180

Query: 181 VFRPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFD 240
           VFRPML+FLR+TGSYLMVNAYPFFAYESN+DVISLDYALFR+NPGVVD+GSGYRYF+LFD
Sbjct: 181 VFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFD 240

Query: 241 AQIDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGT 300
           AQIDAVFAAMSALKYDDI MVV+ETGWPSKGDENEIGAS  NAAAYNGNL+ RILSGGGT
Sbjct: 241 AQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASAANAAAYNGNLIRRILSGGGT 300

Query: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRK 360
           PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE+VYDIPFT E LK+Y DKPS  
Sbjct: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPA 360

Query: 361 PISAPTGAPVSG-GDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVG 420
           P +   G   SG GDGGVSKSQTGNTWCVASGEAG+EKLQAGLDYACGEGGADCRPIQ  
Sbjct: 361 PATGGDGQTGSGHGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 475
           ATCYNPNTL+AHASYAFNSYYQKN RK+GTCYFGGAAYVVTQPPK+GSCEFPTGY
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNVRKIGTCYFGGAAYVVTQPPKFGSCEFPTGY 473

BLAST of Cmc05g0125311 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 709.1 bits (1829), Expect = 2.3e-204
Identity = 344/471 (73.04%), Postives = 409/471 (86.84%), Query Frame = 0

Query: 14  LLFLSPF--AESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDTDPAVLRALSGS 73
           LL LSPF  +++GSIGVNYGRI+++LPSA +VV+LLKS G+ RVK++D DP+VL+ALSGS
Sbjct: 11  LLLLSPFSLSDAGSIGVNYGRISDELPSAFKVVQLLKSQGITRVKIFDADPSVLKALSGS 70

Query: 74  GIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSYL 133
           GIKVTVDLPNELLF+A++R SFA +WV++NVAAY+PST+IE+IAVGNEVFVD HNTTS+L
Sbjct: 71  GIKVTVDLPNELLFSAAKRTSFAVSWVKRNVAAYHPSTQIESIAVGNEVFVDTHNTTSFL 130

Query: 134 VPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVETVFRPMLDFLRR 193
           +PAM+NIH+AL+ +NL S I +SSP+ALSALQNSYP+S+GSFRPEL+++V +PMLDFLR 
Sbjct: 131 IPAMRNIHKALMSFNLHSDIKISSPLALSALQNSYPSSSGSFRPELIDSVIKPMLDFLRE 190

Query: 194 TGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFDAQIDAVFAAMS 253
           TGS LM+N YPFFAYE N+DVI LDYAL R+NPG+VD+G+G RYF+LFDAQIDAVFAAMS
Sbjct: 191 TGSRLMINVYPFFAYEGNSDVIPLDYALLRENPGMVDSGNGLRYFNLFDAQIDAVFAAMS 250

Query: 254 ALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGTPLRPKADLTVY 313
           ALKYDDI ++V+ETGWPSKGDENE+GA++ NAA+YNGNL+ RIL+ GGTPLRPKADLTVY
Sbjct: 251 ALKYDDIEIIVTETGWPSKGDENEVGATLANAASYNGNLIRRILTRGGTPLRPKADLTVY 310

Query: 314 LFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRKPISAPTGAPVS 373
           LFALFNENKK GPTSERNYGLF+P+E+KVYDIPFT EGLK Y+D             PV+
Sbjct: 311 LFALFNENKKLGPTSERNYGLFFPDEKKVYDIPFTTEGLKHYRDGGH---------TPVT 370

Query: 374 GGD--------GGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGATCY 433
           GGD        GGVSKS  G TWCVA+G+AG E+LQ GLDYACGEGGADCRPIQ GA CY
Sbjct: 371 GGDQVTKPPMSGGVSKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRPIQPGANCY 430

Query: 434 NPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 475
           +P+TLEAHAS+AFNSYYQK  R  G+CYFGGAAYVV+QPPKYG CEFPTGY
Sbjct: 431 SPDTLEAHASFAFNSYYQKKGRAGGSCYFGGAAYVVSQPPKYGRCEFPTGY 472

BLAST of Cmc05g0125311 vs. TAIR 10
Match: AT5G55180.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 610.9 bits (1574), Expect = 8.6e-175
Identity = 307/472 (65.04%), Postives = 361/472 (76.48%), Query Frame = 0

Query: 10  FSLFLLFLSPF-------AESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDTDP 69
           F L LL LS F       A+SG IGVNYGRIA++LP+  +VV+LLK+ G+ R+K+YDT+ 
Sbjct: 4   FVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYDTET 63

Query: 70  AVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEVFV 129
            VL AL+ SGIKV V LPNE L +A+   S+   WVQ N+  Y P+T+IEAIAVGNEVFV
Sbjct: 64  TVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNEVFV 123

Query: 130 DPHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVETVF 189
           DP NTT+YLVPAMKN+  +LVK+NLD  I +SSPIALSAL +SYP SAGSF+PEL+E V 
Sbjct: 124 DPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIEPVI 183

Query: 190 RPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFDAQ 249
           +PMLD LR+T S+LMVNAYPFFAY +N D ISLDYALF++N G VD+G+G +Y SL DAQ
Sbjct: 184 KPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLLDAQ 243

Query: 250 IDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGTPL 309
           IDAVFAAMSA+ ++D+ +VV+ETGWPS GDENEIGA   NAAAYNG LV R+L+G GTPL
Sbjct: 244 IDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNGTPL 303

Query: 310 RPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRKPI 369
           +PK  L VYLFALFNEN+K GPTSERNYGLFYPNE KVYD+    +      +K    P 
Sbjct: 304 KPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSLNGKSTPVNDNKEKVVP- 363

Query: 370 SAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGATC 429
                         V  S  G TWCVA+G+  +EKLQ GLDYACGEGGADCRPIQ GATC
Sbjct: 364 --------------VKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGATC 423

Query: 430 YNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 475
           YNP +LEAHASYAFNSYYQKN R VGTC FGGAAYVV+QPPKYG CEFPTG+
Sbjct: 424 YNPESLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPPKYGKCEFPTGH 460

BLAST of Cmc05g0125311 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 601.3 bits (1549), Expect = 6.8e-172
Identity = 302/466 (64.81%), Postives = 363/466 (77.90%), Query Frame = 0

Query: 10  FSLFLLFLSPF-AESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDTDPAVLRAL 69
           + L L FLS   A SG +GVNYGRIAN+LPS  +VV LLKS G+ R+K++DTD  VL AL
Sbjct: 8   YFLILSFLSAIDAHSGMVGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDTDKNVLTAL 67

Query: 70  SGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEVFVDPHNTT 129
           + S IKV V LPNELL +A+   SFA NW++ ++  Y+P+TEIEAIAVGNEVFVDP   T
Sbjct: 68  ANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEVFVDP-TIT 127

Query: 130 SYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVETVFRPMLDF 189
            YLV AMKNIH +LVKY LD  I +SSPIALSAL NSYP S+GSF+PEL+E V +PML  
Sbjct: 128 PYLVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIEPVVKPMLAL 187

Query: 190 LRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFDAQIDAVFA 249
           L++T SYLMVNAYPFFAY +N D ISLDYALF++N G +D+G+G +Y SLFDAQIDAV+A
Sbjct: 188 LQQTSSYLMVNAYPFFAYAANADKISLDYALFKENAGNIDSGTGLKYNSLFDAQIDAVYA 247

Query: 250 AMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGTPLRPKADL 309
           A+SA+ +  + ++V+ETGWPS GDENEIGAS  NAAAYN  LV R+L+G GTPLRP   L
Sbjct: 248 ALSAVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGKGTPLRPTEPL 307

Query: 310 TVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRKPISAPTGA 369
            VYLFALFNEN+K GPTSERNYGLFYPNE KVY++PFT +                 +  
Sbjct: 308 NVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFTKK-----------------STT 367

Query: 370 PVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGATCYNPNTL 429
           PV+G  G V  +  G+TWCV++GE  +EKLQ  LDYACGEGGADCRPIQ GATCY+P +L
Sbjct: 368 PVNGNRGKVPVTHEGHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCYHPESL 427

Query: 430 EAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSCEFPTGY 475
           EAHASYAFNSYYQKN+R+VGTC+FGGAA+VVTQPP+YG CEFPTG+
Sbjct: 428 EAHASYAFNSYYQKNSRRVGTCFFGGAAHVVTQPPRYGKCEFPTGH 455

BLAST of Cmc05g0125311 vs. TAIR 10
Match: AT5G55180.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 588.6 bits (1516), Expect = 4.6e-168
Identity = 298/461 (64.64%), Postives = 351/461 (76.14%), Query Frame = 0

Query: 10  FSLFLLFLSPF-------AESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDTDP 69
           F L LL LS F       A+SG IGVNYGRIA++LP+  +VV+LLK+ G+ R+K+YDT+ 
Sbjct: 4   FVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYDTET 63

Query: 70  AVLRALSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEVFV 129
            VL AL+ SGIKV V LPNE L +A+   S+   WVQ N+  Y P+T+IEAIAVGNEVFV
Sbjct: 64  TVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNEVFV 123

Query: 130 DPHNTTSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVETVF 189
           DP NTT+YLVPAMKN+  +LVK+NLD  I +SSPIALSAL +SYP SAGSF+PEL+E V 
Sbjct: 124 DPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIEPVI 183

Query: 190 RPMLDFLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFDAQ 249
           +PMLD LR+T S+LMVNAYPFFAY +N D ISLDYALF++N G VD+G+G +Y SL DAQ
Sbjct: 184 KPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLLDAQ 243

Query: 250 IDAVFAAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGTPL 309
           IDAVFAAMSA+ ++D+ +VV+ETGWPS GDENEIGA   NAAAYNG LV R+L+G GTPL
Sbjct: 244 IDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNGTPL 303

Query: 310 RPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRKPI 369
           +PK  L VYLFALFNEN+K GPTSERNYGLFYPNE KVYD+    +      +K    P 
Sbjct: 304 KPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSLNGKSTPVNDNKEKVVP- 363

Query: 370 SAPTGAPVSGGDGGVSKSQTGNTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGATC 429
                         V  S  G TWCVA+G+  +EKLQ GLDYACGEGGADCRPIQ GATC
Sbjct: 364 --------------VKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGATC 423

Query: 430 YNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPP 464
           YNP +LEAHASYAFNSYYQKN R VGTC FGGAAYVV+QPP
Sbjct: 424 YNPESLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPP 449

BLAST of Cmc05g0125311 vs. TAIR 10
Match: AT4G29360.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 364.8 bits (935), Expect = 1.1e-100
Identity = 190/466 (40.77%), Postives = 285/466 (61.16%), Query Frame = 0

Query: 8   WFFSLFLLFLSPFAESGSIGVNYGRIANDLPSAVQVVKLLKSNGLQRVKVYDTDPAVLRA 67
           W F   L FL+ F  +  IG+ YGR A++LPS  +V +L++   ++ V++YD +  VL+A
Sbjct: 10  WIFVSILAFLN-FGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKA 69

Query: 68  LSGSGIKVTVDLPNELLFAASQRLSFAYNWVQKNVAAYYPSTEIEAIAVGNEVFVDPHNT 127
            + +GI++ + +PN  L A +Q  S    W+  N+  YYPST+I +I+VG EV   P N 
Sbjct: 70  FANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNA 129

Query: 128 TSYLVPAMKNIHQALVKYNLDSKIMVSSPIALSALQNSYPASAGSFRPELVETVFRPMLD 187
           T  ++PAM+NIH AL K  LD KI +SS  +L+ L  S+P S+ SF  +      +PML+
Sbjct: 130 TGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HSAFLKPMLE 189

Query: 188 FLRRTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDTGSGYRYFSLFDAQIDAVF 247
           FL    S  M++ YP++AY  +T+ + L+YALF  +  VVD  +G  Y ++FDAQ+DA++
Sbjct: 190 FLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIY 249

Query: 248 AAMSALKYDDINMVVSETGWPSKGDENEIGASVENAAAYNGNLVHRILSGGGTPLRPKAD 307
            A++A+ +  + ++V+E+GWPSKG   E  A+ ENA AYN NL+  ++   GTP +P  +
Sbjct: 250 FALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEE 309

Query: 308 LTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTAEGLKEYKDKPSRKPIS-APT 367
           + VYLF+LFNEN+K G  SERN+G+FY N   VY + FT E         S    S +P+
Sbjct: 310 IDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPS 369

Query: 368 GAPVSGGDGGVSKSQTG----NTWCVASGEAGREKLQAGLDYACGEGGADCRPIQVGATC 427
            +P+  G+  V+    G      WC+AS +A   +LQ  LD+ACG G  DC  +Q    C
Sbjct: 370 SSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPC 429

Query: 428 YNPNTLEAHASYAFNSYYQKNNRKVGTCYFGGAAYVVTQPPKYGSC 469
           + P+T+ +HASYAFN+YYQ++      C F GA+  V + P YG+C
Sbjct: 430 FEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNC 473

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008449478.17.1e-27299.58PREDICTED: glucan endo-1,3-beta-glucosidase 12 [Cucumis melo] >KAA0061649.1 gluc... [more]
XP_004140121.12.1e-25291.82glucan endo-1,3-beta-glucosidase 12 [Cucumis sativus] >KGN47963.1 hypothetical p... [more]
XP_038886813.12.7e-23988.03LOW QUALITY PROTEIN: glucan endo-1,3-beta-glucosidase 12 [Benincasa hispida][more]
XP_022952844.12.7e-23988.63glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata][more]
KAG6572415.13.9e-23888.00Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. soro... [more]
Match NameE-valueIdentityDescription
Q8VYE51.5e-9940.77Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9FJU95.9e-9639.53Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Q9ZU911.7e-9540.76Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ... [more]
Q9C7U51.1e-9440.92Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ... [more]
Q9M0693.3e-9441.08Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Match NameE-valueIdentityDescription
A0A5D3C2063.4e-27299.58(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BMR43.4e-27299.58(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103491351 PE=3 SV... [more]
A0A0A0KEL01.0e-25291.82(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G421570 PE=3... [more]
A0A6J1GMW61.3e-23988.63(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111455410 P... [more]
A0A6J1I0V34.2e-23888.21(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111468418 PE=... [more]
Match NameE-valueIdentityDescription
AT2G05790.12.3e-20473.04O-Glycosyl hydrolases family 17 protein [more]
AT5G55180.18.6e-17565.04O-Glycosyl hydrolases family 17 protein [more]
AT4G26830.16.8e-17264.81O-Glycosyl hydrolases family 17 protein [more]
AT5G55180.24.6e-16864.64O-Glycosyl hydrolases family 17 protein [more]
AT4G29360.11.1e-10040.77O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 385..470
e-value: 5.8E-49
score: 178.6
IPR012946X8 domainPFAMPF07983X8coord: 385..456
e-value: 2.8E-22
score: 79.2
NoneNo IPR availableGENE3D1.20.58.1040coord: 380..470
e-value: 2.4E-10
score: 42.6
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 24..353
e-value: 4.6E-126
score: 422.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 355..378
NoneNo IPR availablePANTHERPTHR32227:SF350O-GLYCOSYL HYDROLASES FAMILY 17 PROTEIN-RELATEDcoord: 11..467
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 26..345
e-value: 6.8E-91
score: 305.1
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 258..271
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 11..467
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 26..344

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc05g0125311.1Cmc05g0125311.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds