Cmc04g0101131 (gene) Melon (Charmono) v1.1

Overview
NameCmc04g0101131
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionPyruvate kinase
LocationCMiso1.1chr04: 17365196 .. 17365670 (-)
RNA-Seq ExpressionCmc04g0101131
SyntenyCmc04g0101131
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACACTTTGCATGCTGCTGAAATTCACATTGATCTTCCAACACTTACTGAGAAAGACAGAGTAACATTCTTCTGTTTCCTGTTTGTTATGCCTGTTAAGTCTCTCTAATTGATTGCTTGCAATTAGCTTATACAACCTTAACATGATTGGTAGCTCATAGTATTTTTTGATGCTGACTGGTGACTTTAGATCATAGCTACGTGGGGAGTAAAAAACAAGATTGACTTCCTCTCTCTGTCACATGCTAGACATGTGGAAGATGTTCGACAAGTATTATTTCCTTGCATTGAGTCTTCACTTTAAACAAATGATCGACTGTGGGTGATTTGACTATGTTGATTATTAGAATAGAGGATATTGATTGCTAAATTAAATTTTTATGTAGGCTCGACAATTTCTTTCTAAGCTAGGTGACCTTAACCAAACTCAAATATTTGCAAAGATTGAAAGTGTAGAGGTAATTCATTGTTAG

mRNA sequence

ATGTACACTTTGCATGCTGCTGAAATTCACATTGATCTTCCAACACTTACTGAGAAAGACAGAATCATAGCTACGTGGGGAGTAAAAAACAAGATTGACTTCCTCTCTCTGTCACATGCTAGACATGTGGAAGATGTTCGACAAGCTCGACAATTTCTTTCTAAGCTAGGTGACCTTAACCAAACTCAAATATTTGCAAAGATTGAAAGTGTAGAGGTAATTCATTGTTAG

Coding sequence (CDS)

ATGTACACTTTGCATGCTGCTGAAATTCACATTGATCTTCCAACACTTACTGAGAAAGACAGAATCATAGCTACGTGGGGAGTAAAAAACAAGATTGACTTCCTCTCTCTGTCACATGCTAGACATGTGGAAGATGTTCGACAAGCTCGACAATTTCTTTCTAAGCTAGGTGACCTTAACCAAACTCAAATATTTGCAAAGATTGAAAGTGTAGAGGTAATTCATTGTTAG

Protein sequence

MYTLHAAEIHIDLPTLTEKDRIIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDLNQTQIFAKIESVEVIHC
Homology
BLAST of Cmc04g0101131 vs. NCBI nr
Match: KAA0036675.1 (pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa])

HSP 1 Score: 140.2 bits (352), Expect = 7.4e-30
Identity = 73/84 (86.90%), Postives = 73/84 (86.90%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-----------IIATWGVKNKIDFLSLSHARHVEDVRQA 60
           MYTLHAAEIHIDLPTLTEKDR           IIATWGVKNKIDFLSLSHARHVEDVRQA
Sbjct: 51  MYTLHAAEIHIDLPTLTEKDRVTFFCFLFVMPIIATWGVKNKIDFLSLSHARHVEDVRQA 110

Query: 61  RQFLSKLGDLNQTQIFAKIESVEV 74
           RQFLSKLGDLNQTQIFAKIESVEV
Sbjct: 111 RQFLSKLGDLNQTQIFAKIESVEV 134

BLAST of Cmc04g0101131 vs. NCBI nr
Match: KAA0025298.1 (pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa])

HSP 1 Score: 140.2 bits (352), Expect = 7.4e-30
Identity = 70/73 (95.89%), Postives = 71/73 (97.26%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           MYTLHAAEIHIDLPTLTEKD+ IIATWGVKNKIDFLSLSHARH EDVRQARQFLSKLGDL
Sbjct: 189 MYTLHAAEIHIDLPTLTEKDKEIIATWGVKNKIDFLSLSHARHAEDVRQARQFLSKLGDL 248

Query: 61  NQTQIFAKIESVE 73
           NQTQIFAKIESVE
Sbjct: 249 NQTQIFAKIESVE 261

BLAST of Cmc04g0101131 vs. NCBI nr
Match: XP_008462524.1 (PREDICTED: pyruvate kinase 2, cytosolic-like [Cucumis melo])

HSP 1 Score: 140.2 bits (352), Expect = 7.4e-30
Identity = 70/73 (95.89%), Postives = 71/73 (97.26%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           MYTLHAAEIHIDLPTLTEKD+ IIATWGVKNKIDFLSLSHARH EDVRQARQFLSKLGDL
Sbjct: 189 MYTLHAAEIHIDLPTLTEKDKEIIATWGVKNKIDFLSLSHARHAEDVRQARQFLSKLGDL 248

Query: 61  NQTQIFAKIESVE 73
           NQTQIFAKIESVE
Sbjct: 249 NQTQIFAKIESVE 261

BLAST of Cmc04g0101131 vs. NCBI nr
Match: TYK07370.1 (pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa])

HSP 1 Score: 140.2 bits (352), Expect = 7.4e-30
Identity = 70/73 (95.89%), Postives = 71/73 (97.26%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           MYTLHAAEIHIDLPTLTEKD+ IIATWGVKNKIDFLSLSHARH EDVRQARQFLSKLGDL
Sbjct: 189 MYTLHAAEIHIDLPTLTEKDKEIIATWGVKNKIDFLSLSHARHAEDVRQARQFLSKLGDL 248

Query: 61  NQTQIFAKIESVE 73
           NQTQIFAKIESVE
Sbjct: 249 NQTQIFAKIESVE 261

BLAST of Cmc04g0101131 vs. NCBI nr
Match: XP_011657683.1 (pyruvate kinase 2, cytosolic [Cucumis sativus] >KGN48226.1 hypothetical protein Csa_003160 [Cucumis sativus])

HSP 1 Score: 139.0 bits (349), Expect = 1.6e-29
Identity = 69/73 (94.52%), Postives = 71/73 (97.26%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           MYTLHAAEIHIDLPTLT+KD+ IIATWGVKNKIDFLSLSHARH EDVRQARQFLSKLGDL
Sbjct: 189 MYTLHAAEIHIDLPTLTDKDKEIIATWGVKNKIDFLSLSHARHAEDVRQARQFLSKLGDL 248

Query: 61  NQTQIFAKIESVE 73
           NQTQIFAKIESVE
Sbjct: 249 NQTQIFAKIESVE 261

BLAST of Cmc04g0101131 vs. ExPASy Swiss-Prot
Match: B8BM17 (Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_37456 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 5.7e-25
Identity = 53/73 (72.60%), Postives = 66/73 (90.41%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           ++TLH ++IHIDLPTL+++D+ +I  WG  NKIDFLSLS+ RHVEDVRQAR+FLSKLGDL
Sbjct: 189 LFTLHCSQIHIDLPTLSDEDKEVIRKWGAPNKIDFLSLSYTRHVEDVRQAREFLSKLGDL 248

Query: 61  NQTQIFAKIESVE 73
           +QTQIFAKIE+VE
Sbjct: 249 SQTQIFAKIENVE 261

BLAST of Cmc04g0101131 vs. ExPASy Swiss-Prot
Match: Q2QXR8 (Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0145700 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 5.7e-25
Identity = 53/73 (72.60%), Postives = 66/73 (90.41%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           ++TLH ++IHIDLPTL+++D+ +I  WG  NKIDFLSLS+ RHVEDVRQAR+FLSKLGDL
Sbjct: 189 LFTLHCSQIHIDLPTLSDEDKEVIRKWGAPNKIDFLSLSYTRHVEDVRQAREFLSKLGDL 248

Query: 61  NQTQIFAKIESVE 73
           +QTQIFAKIE+VE
Sbjct: 249 SQTQIFAKIENVE 261

BLAST of Cmc04g0101131 vs. ExPASy Swiss-Prot
Match: B8BJ39 (Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_35105 PE=3 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.7e-24
Identity = 52/73 (71.23%), Postives = 65/73 (89.04%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           ++TLH ++IHIDLPTL+++D+ +I  WG  NKIDFLSLS+ RH EDVRQAR+FLSKLGDL
Sbjct: 189 LFTLHCSQIHIDLPTLSDEDKEVIRRWGAPNKIDFLSLSYTRHAEDVRQAREFLSKLGDL 248

Query: 61  NQTQIFAKIESVE 73
           +QTQIFAKIE+VE
Sbjct: 249 SQTQIFAKIENVE 261

BLAST of Cmc04g0101131 vs. ExPASy Swiss-Prot
Match: Q2RAK2 (Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0148500 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.7e-24
Identity = 52/73 (71.23%), Postives = 65/73 (89.04%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           ++TLH ++IHIDLPTL+++D+ +I  WG  NKIDFLSLS+ RH EDVRQAR+FLSKLGDL
Sbjct: 189 LFTLHCSQIHIDLPTLSDEDKEVIRRWGAPNKIDFLSLSYTRHAEDVRQAREFLSKLGDL 248

Query: 61  NQTQIFAKIESVE 73
           +QTQIFAKIE+VE
Sbjct: 249 SQTQIFAKIENVE 261

BLAST of Cmc04g0101131 vs. ExPASy Swiss-Prot
Match: Q7RVA8 (Pyruvate kinase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=ace-8 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.5e-06
Identity = 26/60 (43.33%), Postives = 38/60 (63.33%), Query Frame = 0

Query: 11  IDLPTLTEKDRIIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDLNQTQIFAKIES 70
           +DLP L+EKD+    +GVKNK+D +  S  R  +D++  R+ L +  D  Q QI AKIE+
Sbjct: 199 VDLPALSEKDKADLRFGVKNKVDMVFASFIRRGQDIKDIREVLGE--DGKQIQIIAKIEN 256

BLAST of Cmc04g0101131 vs. ExPASy TrEMBL
Match: A0A5D3C7Q7 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00900 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 3.6e-30
Identity = 70/73 (95.89%), Postives = 71/73 (97.26%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           MYTLHAAEIHIDLPTLTEKD+ IIATWGVKNKIDFLSLSHARH EDVRQARQFLSKLGDL
Sbjct: 189 MYTLHAAEIHIDLPTLTEKDKEIIATWGVKNKIDFLSLSHARHAEDVRQARQFLSKLGDL 248

Query: 61  NQTQIFAKIESVE 73
           NQTQIFAKIESVE
Sbjct: 249 NQTQIFAKIESVE 261

BLAST of Cmc04g0101131 vs. ExPASy TrEMBL
Match: A0A5A7SGM8 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001520 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 3.6e-30
Identity = 70/73 (95.89%), Postives = 71/73 (97.26%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           MYTLHAAEIHIDLPTLTEKD+ IIATWGVKNKIDFLSLSHARH EDVRQARQFLSKLGDL
Sbjct: 189 MYTLHAAEIHIDLPTLTEKDKEIIATWGVKNKIDFLSLSHARHAEDVRQARQFLSKLGDL 248

Query: 61  NQTQIFAKIESVE 73
           NQTQIFAKIESVE
Sbjct: 249 NQTQIFAKIESVE 261

BLAST of Cmc04g0101131 vs. ExPASy TrEMBL
Match: A0A5A7T5E1 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold510G00160 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 3.6e-30
Identity = 73/84 (86.90%), Postives = 73/84 (86.90%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-----------IIATWGVKNKIDFLSLSHARHVEDVRQA 60
           MYTLHAAEIHIDLPTLTEKDR           IIATWGVKNKIDFLSLSHARHVEDVRQA
Sbjct: 51  MYTLHAAEIHIDLPTLTEKDRVTFFCFLFVMPIIATWGVKNKIDFLSLSHARHVEDVRQA 110

Query: 61  RQFLSKLGDLNQTQIFAKIESVEV 74
           RQFLSKLGDLNQTQIFAKIESVEV
Sbjct: 111 RQFLSKLGDLNQTQIFAKIESVEV 134

BLAST of Cmc04g0101131 vs. ExPASy TrEMBL
Match: A0A1S3CH64 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103500862 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 3.6e-30
Identity = 70/73 (95.89%), Postives = 71/73 (97.26%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           MYTLHAAEIHIDLPTLTEKD+ IIATWGVKNKIDFLSLSHARH EDVRQARQFLSKLGDL
Sbjct: 189 MYTLHAAEIHIDLPTLTEKDKEIIATWGVKNKIDFLSLSHARHAEDVRQARQFLSKLGDL 248

Query: 61  NQTQIFAKIESVE 73
           NQTQIFAKIESVE
Sbjct: 249 NQTQIFAKIESVE 261

BLAST of Cmc04g0101131 vs. ExPASy TrEMBL
Match: A0A0A0KKD4 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_6G449820 PE=3 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 8.0e-30
Identity = 69/73 (94.52%), Postives = 71/73 (97.26%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           MYTLHAAEIHIDLPTLT+KD+ IIATWGVKNKIDFLSLSHARH EDVRQARQFLSKLGDL
Sbjct: 189 MYTLHAAEIHIDLPTLTDKDKEIIATWGVKNKIDFLSLSHARHAEDVRQARQFLSKLGDL 248

Query: 61  NQTQIFAKIESVE 73
           NQTQIFAKIESVE
Sbjct: 249 NQTQIFAKIESVE 261

BLAST of Cmc04g0101131 vs. TAIR 10
Match: AT3G52990.1 (Pyruvate kinase family protein )

HSP 1 Score: 117.1 bits (292), Expect = 6.2e-27
Identity = 53/73 (72.60%), Postives = 67/73 (91.78%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           ++TLH++++HIDLPTLTEKD+ +I+TWGV+NKIDFLSLS+ RH EDVRQ R+ L KLGDL
Sbjct: 189 LFTLHSSQVHIDLPTLTEKDKEVISTWGVQNKIDFLSLSYCRHAEDVRQTREMLKKLGDL 248

Query: 61  NQTQIFAKIESVE 73
           +QTQIFAKIE+VE
Sbjct: 249 SQTQIFAKIENVE 261

BLAST of Cmc04g0101131 vs. TAIR 10
Match: AT3G52990.2 (Pyruvate kinase family protein )

HSP 1 Score: 117.1 bits (292), Expect = 6.2e-27
Identity = 53/73 (72.60%), Postives = 67/73 (91.78%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           ++TLH++++HIDLPTLTEKD+ +I+TWGV+NKIDFLSLS+ RH EDVRQ R+ L KLGDL
Sbjct: 136 LFTLHSSQVHIDLPTLTEKDKEVISTWGVQNKIDFLSLSYCRHAEDVRQTREMLKKLGDL 195

Query: 61  NQTQIFAKIESVE 73
           +QTQIFAKIE+VE
Sbjct: 196 SQTQIFAKIENVE 208

BLAST of Cmc04g0101131 vs. TAIR 10
Match: AT2G36580.1 (Pyruvate kinase family protein )

HSP 1 Score: 111.7 bits (278), Expect = 2.6e-25
Identity = 50/73 (68.49%), Postives = 65/73 (89.04%), Query Frame = 0

Query: 1   MYTLHAAEIHIDLPTLTEKDR-IIATWGVKNKIDFLSLSHARHVEDVRQARQFLSKLGDL 60
           ++TLH +++HID+PTLTEKD+ +I+TWGV+NKIDFLSLS+ RH EDVRQAR+ L+  GDL
Sbjct: 189 LFTLHVSQVHIDMPTLTEKDKEVISTWGVQNKIDFLSLSYCRHAEDVRQARELLNSCGDL 248

Query: 61  NQTQIFAKIESVE 73
           +QTQIFAKIE+ E
Sbjct: 249 SQTQIFAKIENEE 261

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0036675.17.4e-3086.90pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa][more]
KAA0025298.17.4e-3095.89pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa][more]
XP_008462524.17.4e-3095.89PREDICTED: pyruvate kinase 2, cytosolic-like [Cucumis melo][more]
TYK07370.17.4e-3095.89pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa][more]
XP_011657683.11.6e-2994.52pyruvate kinase 2, cytosolic [Cucumis sativus] >KGN48226.1 hypothetical protein ... [more]
Match NameE-valueIdentityDescription
B8BM175.7e-2572.60Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_37456... [more]
Q2QXR85.7e-2572.60Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g01... [more]
B8BJ391.7e-2471.23Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_35105... [more]
Q2RAK21.7e-2471.23Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g01... [more]
Q7RVA83.5e-0643.33Pyruvate kinase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.7... [more]
Match NameE-valueIdentityDescription
A0A5D3C7Q73.6e-3095.89Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G0090... [more]
A0A5A7SGM83.6e-3095.89Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G0015... [more]
A0A5A7T5E13.6e-3086.90Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold510G0016... [more]
A0A1S3CH643.6e-3095.89Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103500862 PE=3 SV=1[more]
A0A0A0KKD48.0e-3094.52Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_6G449820 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G52990.16.2e-2772.60Pyruvate kinase family protein [more]
AT3G52990.26.2e-2772.60Pyruvate kinase family protein [more]
AT2G36580.12.6e-2568.49Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 15..73
e-value: 4.2E-15
score: 57.4
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 8..74
e-value: 4.2E-13
score: 48.9
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 4..14
e-value: 4.2E-15
score: 57.4
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 3..74
NoneNo IPR availablePANTHERPTHR11817:SF105PYRUVATE KINASEcoord: 3..74
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 5..72

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc04g0101131.1Cmc04g0101131.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0043231 intracellular membrane-bounded organelle
cellular_component GO:0016020 membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity