Cmc04g0101121 (gene) Melon (Charmono) v1.1

Overview
NameCmc04g0101121
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionPyruvate kinase family protein
LocationCMiso1.1chr04: 17362823 .. 17363316 (-)
RNA-Seq ExpressionCmc04g0101121
SyntenyCmc04g0101121
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGGAAAGCCTGCTGTTTTAACTCGTGTGGTAGACAGCATGACTAAAAACTTGAGACCTACACGTGCAGTAGCTATTGATGTTGCCAATGTTGTACTTGATGCTGAGTTATTCAATTTTTAAATCTCCTATTGTTTAAAGACTGAAGATGTAGGGATTTTTTAAGTCATGGTTCTTTTAAATCCCTCTCTACTTTCTAAAGCCACAGATCTCATAATAACTGATGTTGAAGATTGGGTTGAACTTTTTTAAAATTTATCTATTTTATGTTCAATTGTATCATTATATTTAGTATAAATCAGGGTTCTTAGTTCCATTCCTGACTGGGGGTATGATTGTATGACGTTTTTTATAACCTGTCTTCTCATGAGTGTGCAAGAAGTGATGCAATTATTCTTGGGGCTGAGACATTGCATGGATTACATCCAGTAGAAACCGTTTCAACCGTTAGTAGAATATGTGCCGAGGTAATTTATGTGTGTATGAGATAG

mRNA sequence

ATGGCTGGAAAGCCTGCTGTTTTAACTCGTGTGGTAGACAGCATGACTAAAAACTTGAGACCTACACGTGCAGTAGCTATTGATGTTGCCAATGTTGTACTTGATGCTGATGATGCAATTATTCTTGGGGCTGAGACATTGCATGGATTACATCCAGTAGAAACCGTTTCAACCGTTAGTAGAATATGTGCCGAGGTAATTTATGTGTGTATGAGATAG

Coding sequence (CDS)

ATGGCTGGAAAGCCTGCTGTTTTAACTCGTGTGGTAGACAGCATGACTAAAAACTTGAGACCTACACGTGCAGTAGCTATTGATGTTGCCAATGTTGTACTTGATGCTGATGATGCAATTATTCTTGGGGCTGAGACATTGCATGGATTACATCCAGTAGAAACCGTTTCAACCGTTAGTAGAATATGTGCCGAGGTAATTTATGTGTGTATGAGATAG

Protein sequence

MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVSRICAEVIYVCMR
Homology
BLAST of Cmc04g0101121 vs. NCBI nr
Match: KAA0054856.1 (pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa])

HSP 1 Score: 118.2 bits (295), Expect = 2.8e-23
Identity = 61/66 (92.42%), Postives = 61/66 (92.42%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKPAVLTRVVDSMT NLRPTRAVA DVAN VLD DDAIILGAETLHGLHPVETVSTVS
Sbjct: 173 MAGKPAVLTRVVDSMTNNLRPTRAVATDVANAVLDGDDAIILGAETLHGLHPVETVSTVS 232

Query: 61  RICAEV 67
           RIC EV
Sbjct: 233 RICVEV 238

BLAST of Cmc04g0101121 vs. NCBI nr
Match: KAA0046375.1 (pyruvate kinase 1, cytosolic [Cucumis melo var. makuwa])

HSP 1 Score: 115.5 bits (288), Expect = 1.8e-22
Identity = 60/66 (90.91%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKP VLTRVVDSMT NLRPT AVA DVAN VLD DDAIILGAETLHGLHPVETVSTVS
Sbjct: 263 MAGKPTVLTRVVDSMTNNLRPTGAVATDVANAVLDGDDAIILGAETLHGLHPVETVSTVS 322

Query: 61  RICAEV 67
           RICAEV
Sbjct: 323 RICAEV 328

BLAST of Cmc04g0101121 vs. NCBI nr
Match: KAA0046382.1 (pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa])

HSP 1 Score: 115.5 bits (288), Expect = 1.8e-22
Identity = 60/66 (90.91%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKP VLTRVVDSMT NLRPT AVA DVAN VLD DDAIILGAETLHGLHPVETVSTVS
Sbjct: 186 MAGKPTVLTRVVDSMTNNLRPTGAVATDVANAVLDGDDAIILGAETLHGLHPVETVSTVS 245

Query: 61  RICAEV 67
           RICAEV
Sbjct: 246 RICAEV 251

BLAST of Cmc04g0101121 vs. NCBI nr
Match: KAA0054848.1 (pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa])

HSP 1 Score: 114.8 bits (286), Expect = 3.1e-22
Identity = 59/65 (90.77%), Postives = 60/65 (92.31%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKPAVLTRVV+SMT NLRPTRAVA DVAN VLD DDAIILGAETLHGLHPVETVSTVS
Sbjct: 206 MAGKPAVLTRVVNSMTNNLRPTRAVATDVANAVLDGDDAIILGAETLHGLHPVETVSTVS 265

Query: 61  RICAE 66
           RIC E
Sbjct: 266 RICVE 270

BLAST of Cmc04g0101121 vs. NCBI nr
Match: KAA0067614.1 (pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa])

HSP 1 Score: 112.5 bits (280), Expect = 1.6e-21
Identity = 58/65 (89.23%), Postives = 59/65 (90.77%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKPAVLTR+VDSMT NLRPTR VA DVAN VLD DDAIILGAETL GLHPVETVSTVS
Sbjct: 202 MAGKPAVLTRMVDSMTNNLRPTRTVATDVANAVLDGDDAIILGAETLRGLHPVETVSTVS 261

Query: 61  RICAE 66
           RICAE
Sbjct: 262 RICAE 266

BLAST of Cmc04g0101121 vs. ExPASy Swiss-Prot
Match: B8BJ39 (Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_35105 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 8.1e-21
Identity = 51/65 (78.46%), Postives = 56/65 (86.15%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKPAV+TRVVDSMT NLRPTRA A DVAN VLD  DAI+LGAETL GL+PVET+S V 
Sbjct: 305 MAGKPAVVTRVVDSMTDNLRPTRAEATDVANAVLDGSDAILLGAETLRGLYPVETISIVG 364

Query: 61  RICAE 66
           +ICAE
Sbjct: 365 KICAE 369

BLAST of Cmc04g0101121 vs. ExPASy Swiss-Prot
Match: Q2RAK2 (Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0148500 PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 8.1e-21
Identity = 51/65 (78.46%), Postives = 56/65 (86.15%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKPAV+TRVVDSMT NLRPTRA A DVAN VLD  DAI+LGAETL GL+PVET+S V 
Sbjct: 305 MAGKPAVVTRVVDSMTDNLRPTRAEATDVANAVLDGSDAILLGAETLRGLYPVETISIVG 364

Query: 61  RICAE 66
           +ICAE
Sbjct: 365 KICAE 369

BLAST of Cmc04g0101121 vs. ExPASy Swiss-Prot
Match: B8BM17 (Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_37456 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 8.1e-21
Identity = 51/65 (78.46%), Postives = 56/65 (86.15%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKPAV+TRVVDSMT NLRPTRA A DVAN VLD  DAI+LGAETL GL+PVET+S V 
Sbjct: 305 MAGKPAVVTRVVDSMTDNLRPTRAEATDVANAVLDGSDAILLGAETLRGLYPVETISIVG 364

Query: 61  RICAE 66
           +ICAE
Sbjct: 365 KICAE 369

BLAST of Cmc04g0101121 vs. ExPASy Swiss-Prot
Match: Q2QXR8 (Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0145700 PE=2 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 8.1e-21
Identity = 51/65 (78.46%), Postives = 56/65 (86.15%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKPAV+TRVVDSMT NLRPTRA A DVAN VLD  DAI+LGAETL GL+PVET+S V 
Sbjct: 305 MAGKPAVVTRVVDSMTDNLRPTRAEATDVANAVLDGSDAILLGAETLRGLYPVETISIVG 364

Query: 61  RICAE 66
           +ICAE
Sbjct: 365 KICAE 369

BLAST of Cmc04g0101121 vs. ExPASy Swiss-Prot
Match: O44006 (Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.5e-11
Identity = 35/66 (53.03%), Postives = 48/66 (72.73%), Query Frame = 0

Query: 1   MAGKPAV-LTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTV 60
           +AGKP +  T++++SMTKN RPTRA A DVAN VLD  D ++L  ET +G  PV+ V+ +
Sbjct: 324 VAGKPVITATQMLESMTKNPRPTRAEAADVANAVLDGTDCVMLSGETANGSFPVQAVTVM 383

Query: 61  SRICAE 66
           SR+C E
Sbjct: 384 SRVCFE 389

BLAST of Cmc04g0101121 vs. ExPASy TrEMBL
Match: A0A5A7UKP0 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold406G00640 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.4e-23
Identity = 61/66 (92.42%), Postives = 61/66 (92.42%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKPAVLTRVVDSMT NLRPTRAVA DVAN VLD DDAIILGAETLHGLHPVETVSTVS
Sbjct: 173 MAGKPAVLTRVVDSMTNNLRPTRAVATDVANAVLDGDDAIILGAETLHGLHPVETVSTVS 232

Query: 61  RICAEV 67
           RIC EV
Sbjct: 233 RICVEV 238

BLAST of Cmc04g0101121 vs. ExPASy TrEMBL
Match: A0A5A7TSJ5 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold149G001130 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 8.9e-23
Identity = 60/66 (90.91%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKP VLTRVVDSMT NLRPT AVA DVAN VLD DDAIILGAETLHGLHPVETVSTVS
Sbjct: 186 MAGKPTVLTRVVDSMTNNLRPTGAVATDVANAVLDGDDAIILGAETLHGLHPVETVSTVS 245

Query: 61  RICAEV 67
           RICAEV
Sbjct: 246 RICAEV 251

BLAST of Cmc04g0101121 vs. ExPASy TrEMBL
Match: A0A5A7TYA8 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold149G00890 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 8.9e-23
Identity = 60/66 (90.91%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKP VLTRVVDSMT NLRPT AVA DVAN VLD DDAIILGAETLHGLHPVETVSTVS
Sbjct: 263 MAGKPTVLTRVVDSMTNNLRPTGAVATDVANAVLDGDDAIILGAETLHGLHPVETVSTVS 322

Query: 61  RICAEV 67
           RICAEV
Sbjct: 323 RICAEV 328

BLAST of Cmc04g0101121 vs. ExPASy TrEMBL
Match: A0A5A7UIR4 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold406G00540 PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.5e-22
Identity = 59/65 (90.77%), Postives = 60/65 (92.31%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKPAVLTRVV+SMT NLRPTRAVA DVAN VLD DDAIILGAETLHGLHPVETVSTVS
Sbjct: 206 MAGKPAVLTRVVNSMTNNLRPTRAVATDVANAVLDGDDAIILGAETLHGLHPVETVSTVS 265

Query: 61  RICAE 66
           RIC E
Sbjct: 266 RICVE 270

BLAST of Cmc04g0101121 vs. ExPASy TrEMBL
Match: A0A5A7VJS4 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold485G00820 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 7.6e-22
Identity = 58/65 (89.23%), Postives = 59/65 (90.77%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKPAVLTR+VDSMT NLRPTR VA DVAN VLD DDAIILGAETL GLHPVETVSTVS
Sbjct: 202 MAGKPAVLTRMVDSMTNNLRPTRTVATDVANAVLDGDDAIILGAETLRGLHPVETVSTVS 261

Query: 61  RICAE 66
           RICAE
Sbjct: 262 RICAE 266

BLAST of Cmc04g0101121 vs. TAIR 10
Match: AT3G52990.1 (Pyruvate kinase family protein )

HSP 1 Score: 105.1 bits (261), Expect = 2.3e-23
Identity = 54/65 (83.08%), Postives = 57/65 (87.69%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKPAVLTRVVDSMT NLRPTRA A DVAN VLD  DAI+LGAETL GL+PVET+STV 
Sbjct: 305 MAGKPAVLTRVVDSMTDNLRPTRAEATDVANAVLDGSDAILLGAETLRGLYPVETISTVG 364

Query: 61  RICAE 66
           RICAE
Sbjct: 365 RICAE 369

BLAST of Cmc04g0101121 vs. TAIR 10
Match: AT3G52990.2 (Pyruvate kinase family protein )

HSP 1 Score: 105.1 bits (261), Expect = 2.3e-23
Identity = 54/65 (83.08%), Postives = 57/65 (87.69%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKPAVLTRVVDSMT NLRPTRA A DVAN VLD  DAI+LGAETL GL+PVET+STV 
Sbjct: 252 MAGKPAVLTRVVDSMTDNLRPTRAEATDVANAVLDGSDAILLGAETLRGLYPVETISTVG 311

Query: 61  RICAE 66
           RICAE
Sbjct: 312 RICAE 316

BLAST of Cmc04g0101121 vs. TAIR 10
Match: AT2G36580.1 (Pyruvate kinase family protein )

HSP 1 Score: 103.6 bits (257), Expect = 6.8e-23
Identity = 53/65 (81.54%), Postives = 56/65 (86.15%), Query Frame = 0

Query: 1   MAGKPAVLTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVS 60
           MAGKPAVLTRVVDSMT NLRPTRA A DVAN VLD  DAI+LGAETL GL+PVET+STV 
Sbjct: 305 MAGKPAVLTRVVDSMTDNLRPTRAEATDVANAVLDGSDAILLGAETLRGLYPVETISTVG 364

Query: 61  RICAE 66
           RIC E
Sbjct: 365 RICCE 369

BLAST of Cmc04g0101121 vs. TAIR 10
Match: AT3G04050.1 (Pyruvate kinase family protein )

HSP 1 Score: 67.0 bits (162), Expect = 7.0e-12
Identity = 36/64 (56.25%), Postives = 44/64 (68.75%), Query Frame = 0

Query: 3   GKPAV-LTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVSR 62
           GKP V  T++++SMTK+ RPTRA A DVAN VLD  D ++L  ET  G HP   V T+SR
Sbjct: 287 GKPIVTATQMLESMTKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPETAVLTMSR 346

Query: 63  ICAE 66
           IC E
Sbjct: 347 ICKE 350

BLAST of Cmc04g0101121 vs. TAIR 10
Match: AT3G25960.1 (Pyruvate kinase family protein )

HSP 1 Score: 64.7 bits (156), Expect = 3.5e-11
Identity = 35/64 (54.69%), Postives = 43/64 (67.19%), Query Frame = 0

Query: 3   GKPAV-LTRVVDSMTKNLRPTRAVAIDVANVVLDADDAIILGAETLHGLHPVETVSTVSR 62
           GKP V  T++++SMT + RPTRA A DVAN VLD  D ++L  ET  G HP   V T+SR
Sbjct: 287 GKPVVTATQMLESMTVSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPETAVLTMSR 346

Query: 63  ICAE 66
           IC E
Sbjct: 347 ICKE 350

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0054856.12.8e-2392.42pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa][more]
KAA0046375.11.8e-2290.91pyruvate kinase 1, cytosolic [Cucumis melo var. makuwa][more]
KAA0046382.11.8e-2290.91pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa][more]
KAA0054848.13.1e-2290.77pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa][more]
KAA0067614.11.6e-2189.23pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
B8BJ398.1e-2178.46Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_35105... [more]
Q2RAK28.1e-2178.46Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g01... [more]
B8BM178.1e-2178.46Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_37456... [more]
Q2QXR88.1e-2178.46Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g01... [more]
O440061.5e-1153.03Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UKP01.4e-2392.42Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold406G0064... [more]
A0A5A7TSJ58.9e-2390.91Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold149G0011... [more]
A0A5A7TYA88.9e-2390.91Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold149G0089... [more]
A0A5A7UIR41.5e-2290.77Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold406G0054... [more]
A0A5A7VJS47.6e-2289.23Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold485G0082... [more]
Match NameE-valueIdentityDescription
AT3G52990.12.3e-2383.08Pyruvate kinase family protein [more]
AT3G52990.22.3e-2383.08Pyruvate kinase family protein [more]
AT2G36580.16.8e-2381.54Pyruvate kinase family protein [more]
AT3G04050.17.0e-1256.25Pyruvate kinase family protein [more]
AT3G25960.13.5e-1154.69Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 2..65
e-value: 4.0E-16
score: 58.9
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 2..66
e-value: 3.9E-20
score: 73.8
NoneNo IPR availablePANTHERPTHR11817:SF98PYRUVATE KINASEcoord: 1..66
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 1..66
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 2..66

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc04g0101121.1Cmc04g0101121.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0043231 intracellular membrane-bounded organelle
cellular_component GO:0016020 membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity