Cmc03g0084341 (gene) Melon (Charmono) v1.1

Overview
NameCmc03g0084341
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionAbscisic acid 8'-hydroxylase 4-like
LocationCMiso1.1chr03: 28592026 .. 28595568 (-)
RNA-Seq ExpressionCmc03g0084341
SyntenyCmc03g0084341
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCAATTGCATACATGTCACCAAATCTTTGGACGACATTTGTTGCTTGTCCTCTCTCTTTTCCTCTCCCTATATAAACCCACTTTCTTTCTTCACTTCTTCTTCAATTTCCTTTGCTTATTAACACAACACAACACAACACAAAAATGGCTTCAATTTTTCTACTCTTTTCCATCTTCCTTCTCCCCCTTCTCCTTCTATTATTCTTCTTCCAAATTCATAACGACAATAAGTTCCTTATCAAGAAACTCCCTCCTGGTTCAATGGGTTGGCCTTTAATCGGGGAAACCTTTCATTTCTATTCTCAACATCCAACCTCCTTTTTCCTTGGAAAGCAAAAAAGGTGGAATTCCCCAATTCAATTTAAATTTATAACAGACACTCACATTGAATTCAAATAATTGATTGATTGATTGATTCATCTGTTGTTACAGATATGGAGAGATATTCAAGACCCACATTCATGGGTGTCCGTGTGTGATGTTAGCGACGCCCGAGGCGGCTCGGTTCGTGCTAGTCACACAAGCTCATCTCTTTAAACCTACATATCCACAAAGCAAAGAGCGATTAATTGGACCATCGGCTCTGTTTTTCCACCAAAGTGAGACCCATTTGCGGCTGCGTAAGCTTATACAAAGCTCCCTCTCACCGGACCCTGTTCGAGCCTTGGTCCCTCACATCCACGCCTTAGCCATCTCTGCATTGCCTTCATCGCTTAGTGGTCATGTCATTGACACTTACCATCAAATGAAGAAGGTGAATTTCAGTTTTTTATTTTATACATGTAAAAAAAAAAAAAAAAAAAAAAAATCTAAATCACAATTGGAGCAGAGATGCTCTGTATACCAAGACAAAAACAGAGCTTTCAATAAGTTTTTGTGTTCTTAATTGAATCAAATGGGGACCAATTATTACTTATCAAATAGTTTAATTTTAGTATATATGCATTTTTTTCTTTTCCCCCAATTTCTCTCGCTGACTTTCATAGCTTTTGTTTTGTTGTCGTCGTCTCTTTTTCCTTCACTCTCCAAAGTTCTCTTTTGAAGTCGGAATTTTGGCAATTTTCGGACATTTGGAAGACACCAACTACAGAGAAGAACTAAGGACAAACTATTCCATAGTGAACAAAGGCTATAATTCTTTCCCCACCAACATTCCGGGAACGCTTTACAAAAAAGCAATTAAAGTAAGTCAATTATGTATGTATATATAAAAAGACATTCTGATTCGGAAATGAATTATTTTATGCTTTTCTTAGAATCTCAGTTCTTTTTCTTTTATTTTTTAAAAAAAAAGGCAAGAAAAAGGCTGGAAGAGATCATAAGCTGTATAATATTAGAGAAAAAGGAGAAGAGATCAAAGGTATTAGGAGAGAAGAATCTATTGTCATGTTTGGTAAAATGGAGAGATGAAAAAGGAGAAAGTTTAAGTGATGAACAGATCGCCGACAACATTATCGGAGTTCTGTTTGCGGCTCAGGACACAACGGCCAGTGTTCTCACTTGGGTTGTTAAATATCTACATGATAACCCCAAAGTTCTTGAAGCTGTCAAGGTAAACTCACACTCACTTCACTCTACCTCTCTATTCATTCATTCATTCATTCATTCAAATGTTTTGAGATTTCATTTTTGCCTCTATATCATTATCAACATAATGTGTTCATTTATAAGTAAATGTTAAGCAGAATTATTAATTACATAATTGATACAAATCAATACTTAGCTAACGCAACCAAGTCCCATGTTTTCTCTCCTATACATATAAAAATATATACCTCTCTACAAAAGTAAATCACATTTGATGGACGAGGCTACTTACTTCATATTTGTTTGTTAGGATAACGTAACAAACCTCGCAAATATCTCCACTATGGTATAATATTGTCCACTTTGAACTTTTATACCAATTGAGATGATTGTTCTTACTTATATGCATAACACTTAATCCCTTATCTAACTGATGTGAGATTTTGGTCGTTTTTTAACCTTGTTAATTTAGATTTAATTTGATATTTGTAATGTTTTCTTATAAAGAAGTAGTAAACGAAAATTGAATGCTTGTTTAGGTATGTTATAATCAATTCATAAAAAAAAGAAGAGGAATAATTAGGTTGCAACGTAGGTTTAGTTCTCCTATATTGTCTTTACTCACTTTATATTTATATATTGATGATGAAATCTTTAATTTAATGTGGTTAAACTAGAAGAGGGTTAGCTAAGAAAAAGAAGAAGAAACATTCGAATATCTTATTATTATTATTATTATTATTTTTTTCCTTTTGTGATACAATTAATTTAAAAGGGAGGTATGAAAGTTAGATAATAAAGGTTAAATGTAGCTAATGAGGGAAGTTAATTTATATAGAAGAGATTGTGTGTTGCAGGTGGAACAAAAGGTAATTGAAGAAGACATTAATGAGGGGAGCCGACCAATGAGATGGTCTCACACACGAAACATGCCCTTTACCAATAAGGTACCACTAAATTCAACCAAATGTTTCTAAATATAATATATAGTTTAGAAAACAAAAAAAAAAAAAAAAAAAAGGTCAATTTAGGGTTTCTTGTTTTATTTTATTTTGTTTTCTAATCAAGATTTTGTTGCATTTTGACTTATGGGTAACGTTATTTATTGGAAAAACAGGTGGTGTTGGAGAGCTTGAGAATGGCTAGTATTATATCATTCACTTTTAGAGAAGCTGTGGCTGATGTTGAATATAAAGGTCAGATATTAAAATCCTAATTTTTGTATTTGTGTTTAAAATATCATAAATAAATTTCCTTTTAGAAAAAGTAGTGTGATAATTAAGTAATTTATAACTCAATTTTCTGACTAATACAACTTTATTTGATAACATATTCCAAATTTTATGTCTATTTTCGATATGTCATAACAAGGCTCATTTAAGTAATTAACCATTCAAATTAATTTTATATTTAATCAATACATTTTTTAATGCAACTTTCCATTAATCAAAATTGTTTTTTTTCATCATAATTAAAAACATGATTTATATAATAATTTTAAAAATCACAACAATCTTAAAATTGTCAGAAATTTCTCAATCTCTACAAGATATAAAATTGAATTTTATGTTTTAAACTACTATGACTTTTAATTTTGTATTTAGTAGGTATTTTTATTTTAAAAAAATGAATAGGTCAATGACTTACTAGATACAAAATTAAAATTGTAGAAGATCTATTTAATGTCAAACTTGAAAGCTTAACAATTTCAAAGTCTTTTAGAGTTTTTATGGAAAGAATTTATATAAAATTCACTACTAATCCAAACATATGTATGATTTCAAATTTCTAGTGCTGAAAAGATGAGATTAAATTTATTGTTTAATGTGTGGAATTTTAGGTCCAAAATTATTTGAAAGGTAGAAATTAAAATGAAAAAGGAAGTTTTATTGGGAATTTTGGTTTGAAATAAGTTTGATTCCTCACCAGGTTATTTAATACCAAAAGGTTGGAAAGTAATGCCTTTTGTTCAGGAATATTCATCACAATCCTGACTACTTTGTTGACCCTCATAA

mRNA sequence

TGCAATTGCATACATGTCACCAAATCTTTGGACGACATTTGTTGCTTGTCCTCTCTCTTTTCCTCTCCCTATATAAACCCACTTTCTTTCTTCACTTCTTCTTCAATTTCCTTTGCTTATTAACACAACACAACACAACACAAAAATGGCTTCAATTTTTCTACTCTTTTCCATCTTCCTTCTCCCCCTTCTCCTTCTATTATTCTTCTTCCAAATTCATAACGACAATAAGTTCCTTATCAAGAAACTCCCTCCTGGTTCAATGGGTTGGCCTTTAATCGGGGAAACCTTTCATTTCTATTCTCAACATCCAACCTCCTTTTTCCTTGGAAAGCAAAAAAGATATGGAGAGATATTCAAGACCCACATTCATGGGTGTCCGTGTGTGATGTTAGCGACGCCCGAGGCGGCTCGGTTCGTGCTAGTCACACAAGCTCATCTCTTTAAACCTACATATCCACAAAGCAAAGAGCGATTAATTGGACCATCGGCTCTGTTTTTCCACCAAAGTGAGACCCATTTGCGGCTGCGTAAGCTTATACAAAGCTCCCTCTCACCGGACCCTGTTCGAGCCTTGGTCCCTCACATCCACGCCTTAGCCATCTCTGCATTGCCTTCATCGCTTAGTGGTCATGTCATTGACACTTACCATCAAATGAAGAAGTTCTCTTTTGAAGTCGGAATTTTGGCAATTTTCGGACATTTGGAAGACACCAACTACAGAGAAGAACTAAGGACAAACTATTCCATAGTGAACAAAGGCTATAATTCTTTCCCCACCAACATTCCGGGAACGCTTTACAAAAAAGCAATTAAAGCAAGAAAAAGGCTGGAAGAGATCATAAGCTGTATAATATTAGAGAAAAAGGAGAAGAGATCAAAGGTATTAGGAGAGAAGAATCTATTGTCATGTTTGGTAAAATGGAGAGATGAAAAAGGAGAAAGTTTAAGTGATGAACAGATCGCCGACAACATTATCGGAGTTCTGTTTGCGGCTCAGGACACAACGGCCAGTGTTCTCACTTGGGTTGTTAAATATCTACATGATAACCCCAAAGTTCTTGAAGCTGTCAAGGTGGAACAAAAGGTAATTGAAGAAGACATTAATGAGGGGAGCCGACCAATGAGATGGTCTCACACACGAAACATGCCCTTTACCAATAAGGTGGTGTTGGAGAGCTTGAGAATGGCTAGTATTATATCATTCACTTTTAGAGAAGCTGTGGCTGATGTTGAATATAAAGGTTATTTAATACCAAAAGGTTGGAAAGTAATGCCTTTTGTTCAGGAATATTCATCACAATCCTGACTACTTTGTTGACCCTCATAA

Coding sequence (CDS)

ATGGCTTCAATTTTTCTACTCTTTTCCATCTTCCTTCTCCCCCTTCTCCTTCTATTATTCTTCTTCCAAATTCATAACGACAATAAGTTCCTTATCAAGAAACTCCCTCCTGGTTCAATGGGTTGGCCTTTAATCGGGGAAACCTTTCATTTCTATTCTCAACATCCAACCTCCTTTTTCCTTGGAAAGCAAAAAAGATATGGAGAGATATTCAAGACCCACATTCATGGGTGTCCGTGTGTGATGTTAGCGACGCCCGAGGCGGCTCGGTTCGTGCTAGTCACACAAGCTCATCTCTTTAAACCTACATATCCACAAAGCAAAGAGCGATTAATTGGACCATCGGCTCTGTTTTTCCACCAAAGTGAGACCCATTTGCGGCTGCGTAAGCTTATACAAAGCTCCCTCTCACCGGACCCTGTTCGAGCCTTGGTCCCTCACATCCACGCCTTAGCCATCTCTGCATTGCCTTCATCGCTTAGTGGTCATGTCATTGACACTTACCATCAAATGAAGAAGTTCTCTTTTGAAGTCGGAATTTTGGCAATTTTCGGACATTTGGAAGACACCAACTACAGAGAAGAACTAAGGACAAACTATTCCATAGTGAACAAAGGCTATAATTCTTTCCCCACCAACATTCCGGGAACGCTTTACAAAAAAGCAATTAAAGCAAGAAAAAGGCTGGAAGAGATCATAAGCTGTATAATATTAGAGAAAAAGGAGAAGAGATCAAAGGTATTAGGAGAGAAGAATCTATTGTCATGTTTGGTAAAATGGAGAGATGAAAAAGGAGAAAGTTTAAGTGATGAACAGATCGCCGACAACATTATCGGAGTTCTGTTTGCGGCTCAGGACACAACGGCCAGTGTTCTCACTTGGGTTGTTAAATATCTACATGATAACCCCAAAGTTCTTGAAGCTGTCAAGGTGGAACAAAAGGTAATTGAAGAAGACATTAATGAGGGGAGCCGACCAATGAGATGGTCTCACACACGAAACATGCCCTTTACCAATAAGGTGGTGTTGGAGAGCTTGAGAATGGCTAGTATTATATCATTCACTTTTAGAGAAGCTGTGGCTGATGTTGAATATAAAGGTTATTTAATACCAAAAGGTTGGAAAGTAATGCCTTTTGTTCAGGAATATTCATCACAATCCTGA

Protein sequence

MASIFLLFSIFLLPLLLLLFFFQIHNDNKFLIKKLPPGSMGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPFVQEYSSQS
Homology
BLAST of Cmc03g0084341 vs. NCBI nr
Match: KAA0060904.1 (abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 685.3 bits (1767), Expect = 3.1e-193
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 40  MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHL 99
           MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHL
Sbjct: 1   MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHL 60

Query: 100 FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS 159
           FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS
Sbjct: 61  FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS 120

Query: 160 LSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLY 219
           LSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLY
Sbjct: 121 LSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLY 180

Query: 220 KKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIG 279
           KKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIG
Sbjct: 181 KKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIG 240

Query: 280 VLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTN 339
           VLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTN
Sbjct: 241 VLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTN 300

Query: 340 KVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMP 379
           KVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMP
Sbjct: 301 KVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMP 339

BLAST of Cmc03g0084341 vs. NCBI nr
Match: XP_038884199.1 (abscisic acid 8'-hydroxylase CYP707A1-like [Benincasa hispida])

HSP 1 Score: 667.2 bits (1720), Expect = 8.8e-188
Identity = 341/378 (90.21%), Postives = 352/378 (93.12%), Query Frame = 0

Query: 1   MASIFLLFSIFLLPLLLLLFFFQIHNDNKFLIKKLPPGSMGWPLIGETFHFYSQHPTSFF 60
           MA  FLL S FLLP  LLLFFFQIH ++KF  KKLPPGSMGWPLIGETFHFYSQ PTSFF
Sbjct: 1   MAPFFLLISFFLLP--LLLFFFQIHKNSKFPNKKLPPGSMGWPLIGETFHFYSQDPTSFF 60

Query: 61  LGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH 120
           LGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH
Sbjct: 61  LGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH 120

Query: 121 QSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSFEVGI 180
           QSETHLRLRKLIQSSLSPDPVRALVP IHALAISALPS L+GH+I+T+HQMKKFSFEVGI
Sbjct: 121 QSETHLRLRKLIQSSLSPDPVRALVPDIHALAISALPSWLNGHLINTFHQMKKFSFEVGI 180

Query: 181 LAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCIILEK 240
           LAIFGHLEDT YREEL TNYSIVNKGYNSFPTNIPGTLYKKA+KARKRL EIISCIILEK
Sbjct: 181 LAIFGHLEDTKYREELMTNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCIILEK 240

Query: 241 KEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLH 300
           +EKR K L EKNLL+CL+KWRDEKGE+LSDEQIADNIIGVLFAAQDTTASVLTWVVKYLH
Sbjct: 241 REKR-KQLEEKNLLACLLKWRDEKGETLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLH 300

Query: 301 DNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTFREAV 360
            N KVLEAVKVEQK IEE INEG RPM WS TRNMPFTNKVVLES RMASIISFTFREAV
Sbjct: 301 YNHKVLEAVKVEQKAIEEGINEGCRPMSWSQTRNMPFTNKVVLESFRMASIISFTFREAV 360

Query: 361 ADVEYKGYLIPKGWKVMP 379
           ADVEYKGYLIPKGWKVMP
Sbjct: 361 ADVEYKGYLIPKGWKVMP 375

BLAST of Cmc03g0084341 vs. NCBI nr
Match: XP_023546528.1 (abscisic acid 8'-hydroxylase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 612.5 bits (1578), Expect = 2.6e-171
Identity = 312/378 (82.54%), Postives = 339/378 (89.68%), Query Frame = 0

Query: 1   MASIFLLFSIFLLPLLLLLFFFQIHNDNKFLIKKLPPGSMGWPLIGETFHFYSQHPTSFF 60
           MA+ F+L SI L P LLL FF++IH +N+   KKLPPGSMGWPLIGETFHFYSQ  T FF
Sbjct: 4   MAATFVLISI-LFP-LLLCFFWKIHKNNRIPNKKLPPGSMGWPLIGETFHFYSQDLT-FF 63

Query: 61  LGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH 120
             KQKRYGEIFKTHIHGCPCVML TPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH
Sbjct: 64  FRKQKRYGEIFKTHIHGCPCVMLTTPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH 123

Query: 121 QSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSFEVGI 180
           QSETHLRLRKLIQSSLSPDPVR LVPHIHALAIS LPS L+GH+I+T HQMKKFSFEVGI
Sbjct: 124 QSETHLRLRKLIQSSLSPDPVRTLVPHIHALAISTLPSWLNGHLINTLHQMKKFSFEVGI 183

Query: 181 LAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCIILEK 240
           L IFGHLE+ NYR+EL  NYSIVNKGYNSFPTN PG++YKKA+KAR RL EII+CII EK
Sbjct: 184 LVIFGHLENANYRDELLKNYSIVNKGYNSFPTNFPGSIYKKALKARNRLGEIINCIISEK 243

Query: 241 KEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLH 300
           +E+R K L EKNLL+CL+KWRDEKGESLSD+QIADNIIGVLFAAQDTTASVLTWVVKYLH
Sbjct: 244 REQR-KELEEKNLLACLLKWRDEKGESLSDDQIADNIIGVLFAAQDTTASVLTWVVKYLH 303

Query: 301 DNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTFREAV 360
           DN K+LEAVKVEQ+ I+E INEGSR M W+ TRNMPFTNKVVLESLRMA++ISF+FREAV
Sbjct: 304 DNDKILEAVKVEQRAIQEAINEGSRAMSWADTRNMPFTNKVVLESLRMATVISFSFREAV 363

Query: 361 ADVEYKGYLIPKGWKVMP 379
            DVEY GYLIPKGWKVMP
Sbjct: 364 TDVEYNGYLIPKGWKVMP 377

BLAST of Cmc03g0084341 vs. NCBI nr
Match: XP_022962256.1 (abscisic acid 8'-hydroxylase 3-like [Cucurbita moschata])

HSP 1 Score: 610.9 bits (1574), Expect = 7.5e-171
Identity = 310/378 (82.01%), Postives = 337/378 (89.15%), Query Frame = 0

Query: 1   MASIFLLFSIFLLPLLLLLFFFQIHNDNKFLIKKLPPGSMGWPLIGETFHFYSQHPTSFF 60
           MA+ F+L SIF    LLL FF++IH +N+   KKLPPGSMGWPLIGETFHFYSQ  T FF
Sbjct: 1   MAATFVLISIFF--PLLLCFFWKIHKNNRIPNKKLPPGSMGWPLIGETFHFYSQDLT-FF 60

Query: 61  LGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH 120
             KQKRYGEIFKTHIHGCPCVML TPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH
Sbjct: 61  FRKQKRYGEIFKTHIHGCPCVMLTTPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH 120

Query: 121 QSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSFEVGI 180
           QSETHLRLRKLIQSSLSPDPVR LVPHIHALAIS LPS L+GH+I+T HQMKKFSFEVGI
Sbjct: 121 QSETHLRLRKLIQSSLSPDPVRTLVPHIHALAISTLPSWLNGHLINTLHQMKKFSFEVGI 180

Query: 181 LAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCIILEK 240
           L IFGHLE+ NYR+EL  NYSIVNKGYNSFPTN PG++YKKA+KAR RL EII+CII EK
Sbjct: 181 LVIFGHLENANYRDELLKNYSIVNKGYNSFPTNFPGSIYKKALKARNRLGEIINCIISEK 240

Query: 241 KEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLH 300
           +E+R K L EKNLL+CL+KWRDEKGESLSD+QIADNIIGVLFAAQDTTASVLTWVVKYLH
Sbjct: 241 REQR-KELEEKNLLACLLKWRDEKGESLSDDQIADNIIGVLFAAQDTTASVLTWVVKYLH 300

Query: 301 DNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTFREAV 360
           DN K+LEAVK EQ+ I+E INEGSR M W+ TRNMPFTNKVVLESLRMA++ISF+FREAV
Sbjct: 301 DNDKILEAVKAEQRAIQEAINEGSRAMSWADTRNMPFTNKVVLESLRMATVISFSFREAV 360

Query: 361 ADVEYKGYLIPKGWKVMP 379
            DVEY GYLIPKGWKVMP
Sbjct: 361 TDVEYNGYLIPKGWKVMP 374

BLAST of Cmc03g0084341 vs. NCBI nr
Match: XP_022144125.1 (abscisic acid 8'-hydroxylase 4-like [Momordica charantia])

HSP 1 Score: 605.5 bits (1560), Expect = 3.2e-169
Identity = 308/382 (80.63%), Postives = 333/382 (87.17%), Query Frame = 0

Query: 1   MASIFLLFSIFLLPLLLL----LFFFQIHNDNKFLIKKLPPGSMGWPLIGETFHFYSQHP 60
           M  IF+LFSIFL  LLLL    L FF+  N+     KKLPPGSMGWPLIGETFH YS+ P
Sbjct: 1   MGEIFVLFSIFLPTLLLLLLLPLIFFKKENNKG---KKLPPGSMGWPLIGETFHLYSRDP 60

Query: 61  TSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSA 120
           T FFL KQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYP  KERLIGPSA
Sbjct: 61  TIFFLDKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPHGKERLIGPSA 120

Query: 121 LFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSF 180
           LFFHQS+ HLRLRKL+QSSLSPDPVRALVP+I ALA SAL   L GH ++T+ +MKKFSF
Sbjct: 121 LFFHQSDAHLRLRKLVQSSLSPDPVRALVPYIDALASSALAFWLHGHTVNTFEEMKKFSF 180

Query: 181 EVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCI 240
           EVGILAIFGHLED NYR+EL  NYS+VN+GYNSF TNIPGT+YKKA+KARKRL EIISCI
Sbjct: 181 EVGILAIFGHLEDDNYRDELMKNYSMVNRGYNSFLTNIPGTIYKKALKARKRLGEIISCI 240

Query: 241 ILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIGVLFAAQDTTASVLTWVV 300
           I EK++  +K L EKNLL CL+KWRDEKGE+LSDEQIADN+IGVLFAAQDTTASVLTWV+
Sbjct: 241 ISEKRQNTTKKLAEKNLLGCLLKWRDEKGETLSDEQIADNLIGVLFAAQDTTASVLTWVL 300

Query: 301 KYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTF 360
           KYLHDN KVL+AVK EQK IEE IN GSRPM WSHTRNMPFTNKVVLESLRMASII+FTF
Sbjct: 301 KYLHDNRKVLQAVKAEQKAIEEAINGGSRPMSWSHTRNMPFTNKVVLESLRMASIITFTF 360

Query: 361 REAVADVEYKGYLIPKGWKVMP 379
           REAVADVEY GYLIPKGWKVMP
Sbjct: 361 REAVADVEYNGYLIPKGWKVMP 379

BLAST of Cmc03g0084341 vs. ExPASy Swiss-Prot
Match: A9QNE7 (Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A1 PE=2 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 8.4e-133
Identity = 246/378 (65.08%), Postives = 303/378 (80.16%), Query Frame = 0

Query: 4   IFLLFSIFLLPLLLLLFFFQIHND--NKFLIKKLPPGSMGWPLIGETFHFYSQHPTSFFL 63
           IFL  S+F+L  L   F F  +N+  +K    KLPPGSMGWP IGET   YSQ P +FF+
Sbjct: 7   IFLYISMFVLGYLSYYFCFGKNNNSSSKKNAYKLPPGSMGWPYIGETLQLYSQDPNAFFI 66

Query: 64  GKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQ 123
            +Q+R+GEIFKT I GCPCVMLA+PEAARFVLV QA+LFKPTYP+SKE LIG SA+FFHQ
Sbjct: 67  NRQRRFGEIFKTKILGCPCVMLASPEAARFVLVNQANLFKPTYPKSKENLIGQSAIFFHQ 126

Query: 124 SETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSFEVGIL 183
            + H  LRKL+Q+ L+P+ +R  +P+I  L+ISAL S + GHV++TYH+MKKFSFEVGIL
Sbjct: 127 GDYHNHLRKLVQAPLNPESIRNQIPYIEELSISALNSWVGGHVVNTYHEMKKFSFEVGIL 186

Query: 184 AIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCIILEKK 243
           AIFGHL D + +EEL+ NYSIV+KGYNSFP N+PGTLY+KA++ARK+L +I+S II E K
Sbjct: 187 AIFGHL-DGHVKEELKKNYSIVDKGYNSFPINLPGTLYRKALQARKKLGKILSEIIREMK 246

Query: 244 EKRSKVLGEKNLLSCLVKWRDEKG-ESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLH 303
           EK++    EK LLSC +  ++EKG   L+++QIADNIIGVLFAAQDTTASVLTW++KYLH
Sbjct: 247 EKKTL---EKGLLSCFLNAKEEKGFLVLNEDQIADNIIGVLFAAQDTTASVLTWIIKYLH 306

Query: 304 DNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTFREAV 363
           DNPK+LE VK EQKVI +   + +  + W+ TR MP T++VVLE+LRMASIISF FREAV
Sbjct: 307 DNPKLLECVKAEQKVIWQSNEQENHGLTWTQTRKMPITSRVVLETLRMASIISFAFREAV 366

Query: 364 ADVEYKGYLIPKGWKVMP 379
           ADVEYKGYLIPKGWKVMP
Sbjct: 367 ADVEYKGYLIPKGWKVMP 380

BLAST of Cmc03g0084341 vs. ExPASy Swiss-Prot
Match: Q9LJK2 (Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 SV=2)

HSP 1 Score: 454.1 bits (1167), Expect = 1.5e-126
Identity = 235/378 (62.17%), Postives = 293/378 (77.51%), Query Frame = 0

Query: 1   MASIFLLFSIFLLPLLLLLFFFQIHNDNKFLIKKLPPGSMGWPLIGETFHFYSQHPTSFF 60
           MA I+ L  + +L L LLL    +    K    KLPPGSMGWP +GET   YSQ+P  FF
Sbjct: 1   MAEIWFLV-VPILILCLLLVRVIVSKKKKNSRGKLPPGSMGWPYLGETLQLYSQNPNVFF 60

Query: 61  LGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH 120
             KQKRYGEIFKT I G PCVMLA+PEAARFVLVT AH+FKPTYP+SKE+LIGPSALFFH
Sbjct: 61  TSKQKRYGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFH 120

Query: 121 QSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSFEVGI 180
           Q + H  +RKL+QSS  P+ +R L+P I  +A+S+L S  +  ++ TY +MKKF+F+VGI
Sbjct: 121 QGDYHSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGI 180

Query: 181 LAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCIILEK 240
           LAIFGHLE ++Y+E L+ NY+IV+KGYNSFP ++PGT Y KA+ ARK+L+ I+S II E+
Sbjct: 181 LAIFGHLE-SSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICER 240

Query: 241 KEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLH 300
           +EKR+    + + L  L+ +++EKG  L+ EQIADNIIGVLFAAQDTTAS LTW++KYLH
Sbjct: 241 REKRAL---QTDFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLH 300

Query: 301 DNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTFREAV 360
           D+ K+LEAVK EQK I E+ +   +P+ W  TRNMP T+KV++ESLRMASIISFTFREAV
Sbjct: 301 DDQKLLEAVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAV 360

Query: 361 ADVEYKGYLIPKGWKVMP 379
            DVEYKGYLIPKGWKVMP
Sbjct: 361 VDVEYKGYLIPKGWKVMP 373

BLAST of Cmc03g0084341 vs. ExPASy Swiss-Prot
Match: Q0J185 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A7 PE=2 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 2.0e-126
Identity = 229/346 (66.18%), Postives = 272/346 (78.61%), Query Frame = 0

Query: 34  KLPPGSMGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVL 93
           +LPPGSMGWP IGET   YSQ P  FF  KQKRYGEIFKTHI GCPCVMLA+PEAARFVL
Sbjct: 43  RLPPGSMGWPYIGETLQLYSQDPNVFFASKQKRYGEIFKTHILGCPCVMLASPEAARFVL 102

Query: 94  VTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAI 153
           VTQAHLFKPTYP+SKER+IGPSALFFHQ + HLRLRKL+Q  L PD +RALVP + A   
Sbjct: 103 VTQAHLFKPTYPRSKERMIGPSALFFHQGDYHLRLRKLVQGPLGPDALRALVPDVEAAVR 162

Query: 154 SALPSSLSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTN 213
           S L +S  G+V  T+H MK+ SF+VGI+ IFG   D   + ELR NY+IV KGYNSFP +
Sbjct: 163 STL-ASWDGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFPNS 222

Query: 214 IPGTLYKKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWR-DEKGESLSDEQ 273
            PGTLY KAI+AR+RL  ++S I+   +E+R++     +LL CL++ R  + G  L+DEQ
Sbjct: 223 FPGTLYYKAIQARRRLHGVLSDIM---RERRARGEPGSDLLGCLMQSRAGDDGALLTDEQ 282

Query: 274 IADNIIGVLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHT 333
           +ADNIIGVLFAAQDTTASVLTW+VKYLHD+PK+LEAV+ EQ  I    + G  P+ W+ T
Sbjct: 283 VADNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQT 342

Query: 334 RNMPFTNKVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMP 379
           R+M  T+KV+LESLRMASIISFTFREAVADVEYKG+LIPKGWKVMP
Sbjct: 343 RSMALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMP 384

BLAST of Cmc03g0084341 vs. ExPASy Swiss-Prot
Match: A2Z212 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A7 PE=3 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.3e-125
Identity = 228/346 (65.90%), Postives = 272/346 (78.61%), Query Frame = 0

Query: 34  KLPPGSMGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVL 93
           +LPPGSMGWP IGET   YSQ P  FF  KQKRYGEIFKTHI GCPCVMLA+PEAARFVL
Sbjct: 43  RLPPGSMGWPYIGETLQLYSQDPNVFFASKQKRYGEIFKTHILGCPCVMLASPEAARFVL 102

Query: 94  VTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAI 153
           VTQAHLFKPTYP+SKER+IGPSALFF+Q + HLRLRKL+Q  L PD +RALVP + A   
Sbjct: 103 VTQAHLFKPTYPRSKERMIGPSALFFNQGDYHLRLRKLVQGPLGPDALRALVPDVEAAVR 162

Query: 154 SALPSSLSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTN 213
           S L +S  G+V  T+H MK+ SF+VGI+ IFG   D   + ELR NY+IV KGYNSFP +
Sbjct: 163 STL-ASWDGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFPNS 222

Query: 214 IPGTLYKKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWR-DEKGESLSDEQ 273
            PGTLY KAI+AR+RL  ++S I+   +E+R++     +LL CL++ R  + G  L+DEQ
Sbjct: 223 FPGTLYYKAIQARRRLHGVLSDIM---RERRARGEPGSDLLGCLMQSRAGDDGALLTDEQ 282

Query: 274 IADNIIGVLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHT 333
           +ADNIIGVLFAAQDTTASVLTW+VKYLHD+PK+LEAV+ EQ  I    + G  P+ W+ T
Sbjct: 283 VADNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQT 342

Query: 334 RNMPFTNKVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMP 379
           R+M  T+KV+LESLRMASIISFTFREAVADVEYKG+LIPKGWKVMP
Sbjct: 343 RSMALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMP 384

BLAST of Cmc03g0084341 vs. ExPASy Swiss-Prot
Match: Q09J78 (Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A6 PE=2 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 5.0e-125
Identity = 223/349 (63.90%), Postives = 271/349 (77.65%), Query Frame = 0

Query: 34  KLPPGSMGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVL 93
           +LPPGSMGWP +GET   YSQ P  FF  KQKRYGEIFKT++ GCPCVMLA+PEAARFVL
Sbjct: 50  RLPPGSMGWPYVGETLQLYSQDPNVFFASKQKRYGEIFKTNLLGCPCVMLASPEAARFVL 109

Query: 94  VTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAI 153
           V+QA LFKPTYP SKER+IGPSALFFHQ E HLRLR+L+Q++L+PD +RALVP + A   
Sbjct: 110 VSQARLFKPTYPPSKERMIGPSALFFHQGEYHLRLRRLVQAALAPDSLRALVPDVDAAVA 169

Query: 154 SALPSSLSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTN 213
           + L +   GHV  T+H MKK SF+VG++ IFG      +REELRTNYS+V +GYN FP  
Sbjct: 170 ATLAAWSGGHVASTFHAMKKLSFDVGVVTIFGGRLGRRHREELRTNYSVVERGYNCFPNR 229

Query: 214 IPGTLYKKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWRDEKGES----LS 273
            PGTLY KAI+ARKRL  I+S I+    E+R++  G  +LL  L++ RD+        L+
Sbjct: 230 FPGTLYHKAIQARKRLRAILSEIV---AERRARGGGGDDLLGGLMRSRDDGTAGAVALLT 289

Query: 274 DEQIADNIIGVLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRW 333
           D+QIADN++GVLFAAQDTTASVLTW++KYLHD+PK+LEAVK EQ  I      G RP+ W
Sbjct: 290 DDQIADNVVGVLFAAQDTTASVLTWILKYLHDSPKLLEAVKAEQMAIYVANEGGKRPLTW 349

Query: 334 SHTRNMPFTNKVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMP 379
           + TR+M  T++V+LESLRMASIISFTFREAVADVEYKG+LIPKGWKVMP
Sbjct: 350 TQTRSMTLTHQVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMP 395

BLAST of Cmc03g0084341 vs. ExPASy TrEMBL
Match: A0A5A7V0Q4 (Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00370 PE=3 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.5e-193
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 40  MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHL 99
           MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHL
Sbjct: 1   MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHL 60

Query: 100 FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS 159
           FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS
Sbjct: 61  FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS 120

Query: 160 LSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLY 219
           LSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLY
Sbjct: 121 LSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLY 180

Query: 220 KKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIG 279
           KKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIG
Sbjct: 181 KKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIG 240

Query: 280 VLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTN 339
           VLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTN
Sbjct: 241 VLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTN 300

Query: 340 KVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMP 379
           KVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMP
Sbjct: 301 KVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMP 339

BLAST of Cmc03g0084341 vs. ExPASy TrEMBL
Match: A0A0A0LKV6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357280 PE=3 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 6.8e-186
Identity = 328/341 (96.19%), Postives = 336/341 (98.53%), Query Frame = 0

Query: 40  MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHL 99
           MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARF+LVTQAHL
Sbjct: 1   MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFILVTQAHL 60

Query: 100 FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS 159
           FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS
Sbjct: 61  FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS 120

Query: 160 L-SGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTL 219
           L +GH+IDTYHQMKKFSFEVGILAIFGHLE+TNYREELRTNYSIVNKGYNSFPTNIPGTL
Sbjct: 121 LNNGHLIDTYHQMKKFSFEVGILAIFGHLENTNYREELRTNYSIVNKGYNSFPTNIPGTL 180

Query: 220 YKKAIKARKRLEEIISCIILEKKEKRSK-VLGEKNLLSCLVKWRDEKGESLSDEQIADNI 279
           YKKAIKARKRLEEIISCII EKKEKRS+ VLGEKNLLSCLVKWRDEKGESLSDEQIADNI
Sbjct: 181 YKKAIKARKRLEEIISCIISEKKEKRSEVVLGEKNLLSCLVKWRDEKGESLSDEQIADNI 240

Query: 280 IGVLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPF 339
           IGVLFAAQDTTASVLTWVVKYLHDNPK+LEAVKVEQKVIEEDINEGS PMRW+HTR MPF
Sbjct: 241 IGVLFAAQDTTASVLTWVVKYLHDNPKILEAVKVEQKVIEEDINEGSGPMRWAHTRTMPF 300

Query: 340 TNKVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMP 379
           TNKVVLESLR+ASIISFTFREAVADVEYKGYLIPKGWKVMP
Sbjct: 301 TNKVVLESLRLASIISFTFREAVADVEYKGYLIPKGWKVMP 341

BLAST of Cmc03g0084341 vs. ExPASy TrEMBL
Match: A0A6J1HCL5 (abscisic acid 8'-hydroxylase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111462760 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.6e-171
Identity = 310/378 (82.01%), Postives = 337/378 (89.15%), Query Frame = 0

Query: 1   MASIFLLFSIFLLPLLLLLFFFQIHNDNKFLIKKLPPGSMGWPLIGETFHFYSQHPTSFF 60
           MA+ F+L SIF    LLL FF++IH +N+   KKLPPGSMGWPLIGETFHFYSQ  T FF
Sbjct: 1   MAATFVLISIFF--PLLLCFFWKIHKNNRIPNKKLPPGSMGWPLIGETFHFYSQDLT-FF 60

Query: 61  LGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH 120
             KQKRYGEIFKTHIHGCPCVML TPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH
Sbjct: 61  FRKQKRYGEIFKTHIHGCPCVMLTTPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH 120

Query: 121 QSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSFEVGI 180
           QSETHLRLRKLIQSSLSPDPVR LVPHIHALAIS LPS L+GH+I+T HQMKKFSFEVGI
Sbjct: 121 QSETHLRLRKLIQSSLSPDPVRTLVPHIHALAISTLPSWLNGHLINTLHQMKKFSFEVGI 180

Query: 181 LAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCIILEK 240
           L IFGHLE+ NYR+EL  NYSIVNKGYNSFPTN PG++YKKA+KAR RL EII+CII EK
Sbjct: 181 LVIFGHLENANYRDELLKNYSIVNKGYNSFPTNFPGSIYKKALKARNRLGEIINCIISEK 240

Query: 241 KEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLH 300
           +E+R K L EKNLL+CL+KWRDEKGESLSD+QIADNIIGVLFAAQDTTASVLTWVVKYLH
Sbjct: 241 REQR-KELEEKNLLACLLKWRDEKGESLSDDQIADNIIGVLFAAQDTTASVLTWVVKYLH 300

Query: 301 DNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTFREAV 360
           DN K+LEAVK EQ+ I+E INEGSR M W+ TRNMPFTNKVVLESLRMA++ISF+FREAV
Sbjct: 301 DNDKILEAVKAEQRAIQEAINEGSRAMSWADTRNMPFTNKVVLESLRMATVISFSFREAV 360

Query: 361 ADVEYKGYLIPKGWKVMP 379
            DVEY GYLIPKGWKVMP
Sbjct: 361 TDVEYNGYLIPKGWKVMP 374

BLAST of Cmc03g0084341 vs. ExPASy TrEMBL
Match: A0A6J1CQS3 (abscisic acid 8'-hydroxylase 4-like OS=Momordica charantia OX=3673 GN=LOC111013891 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.5e-169
Identity = 308/382 (80.63%), Postives = 333/382 (87.17%), Query Frame = 0

Query: 1   MASIFLLFSIFLLPLLLL----LFFFQIHNDNKFLIKKLPPGSMGWPLIGETFHFYSQHP 60
           M  IF+LFSIFL  LLLL    L FF+  N+     KKLPPGSMGWPLIGETFH YS+ P
Sbjct: 1   MGEIFVLFSIFLPTLLLLLLLPLIFFKKENNKG---KKLPPGSMGWPLIGETFHLYSRDP 60

Query: 61  TSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSA 120
           T FFL KQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYP  KERLIGPSA
Sbjct: 61  TIFFLDKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPHGKERLIGPSA 120

Query: 121 LFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSF 180
           LFFHQS+ HLRLRKL+QSSLSPDPVRALVP+I ALA SAL   L GH ++T+ +MKKFSF
Sbjct: 121 LFFHQSDAHLRLRKLVQSSLSPDPVRALVPYIDALASSALAFWLHGHTVNTFEEMKKFSF 180

Query: 181 EVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCI 240
           EVGILAIFGHLED NYR+EL  NYS+VN+GYNSF TNIPGT+YKKA+KARKRL EIISCI
Sbjct: 181 EVGILAIFGHLEDDNYRDELMKNYSMVNRGYNSFLTNIPGTIYKKALKARKRLGEIISCI 240

Query: 241 ILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIGVLFAAQDTTASVLTWVV 300
           I EK++  +K L EKNLL CL+KWRDEKGE+LSDEQIADN+IGVLFAAQDTTASVLTWV+
Sbjct: 241 ISEKRQNTTKKLAEKNLLGCLLKWRDEKGETLSDEQIADNLIGVLFAAQDTTASVLTWVL 300

Query: 301 KYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTF 360
           KYLHDN KVL+AVK EQK IEE IN GSRPM WSHTRNMPFTNKVVLESLRMASII+FTF
Sbjct: 301 KYLHDNRKVLQAVKAEQKAIEEAINGGSRPMSWSHTRNMPFTNKVVLESLRMASIITFTF 360

Query: 361 REAVADVEYKGYLIPKGWKVMP 379
           REAVADVEY GYLIPKGWKVMP
Sbjct: 361 REAVADVEYNGYLIPKGWKVMP 379

BLAST of Cmc03g0084341 vs. ExPASy TrEMBL
Match: A0A6J1K9Y7 (abscisic acid 8'-hydroxylase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111492434 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.8e-162
Identity = 289/339 (85.25%), Postives = 309/339 (91.15%), Query Frame = 0

Query: 40  MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHL 99
           MGWPLIGETFHFYSQ  T FF  KQKRYGEIFKTHIHGCPCVML TPEAARFVLVTQAHL
Sbjct: 1   MGWPLIGETFHFYSQDLT-FFFRKQKRYGEIFKTHIHGCPCVMLTTPEAARFVLVTQAHL 60

Query: 100 FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS 159
           FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVR LVPHIHALAIS LPS 
Sbjct: 61  FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRTLVPHIHALAISTLPSW 120

Query: 160 LSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLY 219
           L+GH+I+T HQMKKFSFEVGIL IFGHLE+ NYR+EL  NYSIVNKGYNSFPTN PG++Y
Sbjct: 121 LNGHLINTLHQMKKFSFEVGILVIFGHLENANYRDELLKNYSIVNKGYNSFPTNFPGSIY 180

Query: 220 KKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIG 279
           KKA+KAR RL EII+CII EK+E+R K L EKNLL+CL+KWRD KGESLSD+QIADNIIG
Sbjct: 181 KKALKARNRLGEIINCIISEKREQR-KELEEKNLLACLLKWRDAKGESLSDDQIADNIIG 240

Query: 280 VLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTN 339
           VLFAAQDTTASVLTWVVKYLHDN K+LEAVKVEQ+ IEE INEGSR M W+ TRNMPFTN
Sbjct: 241 VLFAAQDTTASVLTWVVKYLHDNDKILEAVKVEQRAIEEAINEGSRAMSWADTRNMPFTN 300

Query: 340 KVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMP 379
           KVVLESLRMA++ISF+FREAV DVEY GYLIPKGWKVMP
Sbjct: 301 KVVLESLRMATVISFSFREAVTDVEYNGYLIPKGWKVMP 337

BLAST of Cmc03g0084341 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 454.1 bits (1167), Expect = 1.1e-127
Identity = 235/378 (62.17%), Postives = 293/378 (77.51%), Query Frame = 0

Query: 1   MASIFLLFSIFLLPLLLLLFFFQIHNDNKFLIKKLPPGSMGWPLIGETFHFYSQHPTSFF 60
           MA I+ L  + +L L LLL    +    K    KLPPGSMGWP +GET   YSQ+P  FF
Sbjct: 1   MAEIWFLV-VPILILCLLLVRVIVSKKKKNSRGKLPPGSMGWPYLGETLQLYSQNPNVFF 60

Query: 61  LGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFH 120
             KQKRYGEIFKT I G PCVMLA+PEAARFVLVT AH+FKPTYP+SKE+LIGPSALFFH
Sbjct: 61  TSKQKRYGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFH 120

Query: 121 QSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSFEVGI 180
           Q + H  +RKL+QSS  P+ +R L+P I  +A+S+L S  +  ++ TY +MKKF+F+VGI
Sbjct: 121 QGDYHSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGI 180

Query: 181 LAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCIILEK 240
           LAIFGHLE ++Y+E L+ NY+IV+KGYNSFP ++PGT Y KA+ ARK+L+ I+S II E+
Sbjct: 181 LAIFGHLE-SSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICER 240

Query: 241 KEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLH 300
           +EKR+    + + L  L+ +++EKG  L+ EQIADNIIGVLFAAQDTTAS LTW++KYLH
Sbjct: 241 REKRAL---QTDFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLH 300

Query: 301 DNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTFREAV 360
           D+ K+LEAVK EQK I E+ +   +P+ W  TRNMP T+KV++ESLRMASIISFTFREAV
Sbjct: 301 DDQKLLEAVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAV 360

Query: 361 ADVEYKGYLIPKGWKVMP 379
            DVEYKGYLIPKGWKVMP
Sbjct: 361 VDVEYKGYLIPKGWKVMP 373

BLAST of Cmc03g0084341 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 380.6 bits (976), Expect = 1.5e-105
Identity = 196/377 (51.99%), Postives = 269/377 (71.35%), Query Frame = 0

Query: 4   IFLLFSIFLLPLLLLLFFFQIHNDNKFLIKK--LPPGSMGWPLIGETFHFYSQHPTSFFL 63
           I  LF       L L F   + +  +F   K  LPPG+MGWP +GETF  YSQ P  FF 
Sbjct: 3   ISALFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQ 62

Query: 64  GKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQ 123
            KQKRYG +FKTH+ GCPCVM+++PEAA+FVLVT++HLFKPT+P SKER++G  A+FFHQ
Sbjct: 63  SKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQ 122

Query: 124 SETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSFEVGIL 183
            + H +LRKL+  +  P+ +R +VP I ++A  +L  S  G +I+TY +MK ++F V +L
Sbjct: 123 GDYHAKLRKLVLRAFMPESIRNMVPDIESIAQDSL-RSWEGTMINTYQEMKTYTFNVALL 182

Query: 184 AIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCIILEKK 243
           +IFG  ++  YRE+L+  Y I+ KGYNS P N+PGTL+ K++KARK L +I++ I+ E++
Sbjct: 183 SIFGK-DEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERR 242

Query: 244 EKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHD 303
           +  S      +LL   +  ++E    L+DEQIADNIIGV+FAA+DTTASV++W++KYL +
Sbjct: 243 QNGS---SHNDLLGSFMGDKEE----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAE 302

Query: 304 NPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTFREAVA 363
           NP VLEAV  EQ  I +D  EG   + W  T+ MP T++V+ E+LR+ASI+SFTFREAV 
Sbjct: 303 NPNVLEAVTEEQMAIRKDKEEG-ESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVE 362

Query: 364 DVEYKGYLIPKGWKVMP 379
           DVEY+GYLIPKGWKV+P
Sbjct: 363 DVEYEGYLIPKGWKVLP 369

BLAST of Cmc03g0084341 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 380.6 bits (976), Expect = 1.5e-105
Identity = 196/377 (51.99%), Postives = 269/377 (71.35%), Query Frame = 0

Query: 4   IFLLFSIFLLPLLLLLFFFQIHNDNKFLIKK--LPPGSMGWPLIGETFHFYSQHPTSFFL 63
           I  LF       L L F   + +  +F   K  LPPG+MGWP +GETF  YSQ P  FF 
Sbjct: 3   ISALFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQ 62

Query: 64  GKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQ 123
            KQKRYG +FKTH+ GCPCVM+++PEAA+FVLVT++HLFKPT+P SKER++G  A+FFHQ
Sbjct: 63  SKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQ 122

Query: 124 SETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSFEVGIL 183
            + H +LRKL+  +  P+ +R +VP I ++A  +L  S  G +I+TY +MK ++F V +L
Sbjct: 123 GDYHAKLRKLVLRAFMPESIRNMVPDIESIAQDSL-RSWEGTMINTYQEMKTYTFNVALL 182

Query: 184 AIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCIILEKK 243
           +IFG  ++  YRE+L+  Y I+ KGYNS P N+PGTL+ K++KARK L +I++ I+ E++
Sbjct: 183 SIFGK-DEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERR 242

Query: 244 EKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHD 303
           +  S      +LL   +  ++E    L+DEQIADNIIGV+FAA+DTTASV++W++KYL +
Sbjct: 243 QNGS---SHNDLLGSFMGDKEE----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAE 302

Query: 304 NPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTFREAVA 363
           NP VLEAV  EQ  I +D  EG   + W  T+ MP T++V+ E+LR+ASI+SFTFREAV 
Sbjct: 303 NPNVLEAVTEEQMAIRKDKEEG-ESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVE 362

Query: 364 DVEYKGYLIPKGWKVMP 379
           DVEY+GYLIPKGWKV+P
Sbjct: 363 DVEYEGYLIPKGWKVLP 369

BLAST of Cmc03g0084341 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 379.0 bits (972), Expect = 4.5e-105
Identity = 196/377 (51.99%), Postives = 270/377 (71.62%), Query Frame = 0

Query: 2   ASIFLLFSIFLLPLLLLLFFFQIHNDNKFLIKKLPPGSMGWPLIGETFHFYSQHPTSFFL 61
           + +FL  S   L L LL F   +   +   +  LPPG+MG+P +GETF  YSQ P  FF 
Sbjct: 4   SGLFLTLSAAALFLCLLRFIAGVRRSSSTKL-PLPPGTMGYPYVGETFQLYSQDPNVFFA 63

Query: 62  GKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQ 121
            KQ+RYG +FKTH+ GCPCVM+++PEAA+FVLVT++HLFKPT+P SKER++G  A+FFHQ
Sbjct: 64  AKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQ 123

Query: 122 SETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSFEVGIL 181
            + H +LRKL+  +  PD +R +VPHI ++A  +L +S  G  ++TY +MK ++F V ++
Sbjct: 124 GDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESL-NSWDGTQLNTYQEMKTYTFNVALI 183

Query: 182 AIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCIILEKK 241
           +I G  ++  YRE+L+  Y I+ KGYNS P N+PGTL+ KA+KARK L +I++ I+ +++
Sbjct: 184 SILGK-DEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRR 243

Query: 242 EKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHD 301
           +  S      +LL   ++  D+ G  L+DEQIADNIIGV+FAA+DTTASVLTW++KYL D
Sbjct: 244 QNPS---SHTDLLGSFME--DKAG--LTDEQIADNIIGVIFAARDTTASVLTWILKYLAD 303

Query: 302 NPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTFREAVA 361
           NP VLEAV  EQ  I +D  EG   + W  T+ MP T +V+ E+LR A+I+SFTFREAV 
Sbjct: 304 NPTVLEAVTEEQMAIRKDKKEG-ESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVE 363

Query: 362 DVEYKGYLIPKGWKVMP 379
           DVEY+GYLIPKGWKV+P
Sbjct: 364 DVEYEGYLIPKGWKVLP 369

BLAST of Cmc03g0084341 vs. TAIR 10
Match: AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 379.0 bits (972), Expect = 4.5e-105
Identity = 196/377 (51.99%), Postives = 270/377 (71.62%), Query Frame = 0

Query: 2   ASIFLLFSIFLLPLLLLLFFFQIHNDNKFLIKKLPPGSMGWPLIGETFHFYSQHPTSFFL 61
           + +FL  S   L L LL F   +   +   +  LPPG+MG+P +GETF  YSQ P  FF 
Sbjct: 4   SGLFLTLSAAALFLCLLRFIAGVRRSSSTKL-PLPPGTMGYPYVGETFQLYSQDPNVFFA 63

Query: 62  GKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQ 121
            KQ+RYG +FKTH+ GCPCVM+++PEAA+FVLVT++HLFKPT+P SKER++G  A+FFHQ
Sbjct: 64  AKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQ 123

Query: 122 SETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSSLSGHVIDTYHQMKKFSFEVGIL 181
            + H +LRKL+  +  PD +R +VPHI ++A  +L +S  G  ++TY +MK ++F V ++
Sbjct: 124 GDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESL-NSWDGTQLNTYQEMKTYTFNVALI 183

Query: 182 AIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLYKKAIKARKRLEEIISCIILEKK 241
           +I G  ++  YRE+L+  Y I+ KGYNS P N+PGTL+ KA+KARK L +I++ I+ +++
Sbjct: 184 SILGK-DEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRR 243

Query: 242 EKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHD 301
           +  S      +LL   ++  D+ G  L+DEQIADNIIGV+FAA+DTTASVLTW++KYL D
Sbjct: 244 QNPS---SHTDLLGSFME--DKAG--LTDEQIADNIIGVIFAARDTTASVLTWILKYLAD 303

Query: 302 NPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTNKVVLESLRMASIISFTFREAVA 361
           NP VLEAV  EQ  I +D  EG   + W  T+ MP T +V+ E+LR A+I+SFTFREAV 
Sbjct: 304 NPTVLEAVTEEQMAIRKDKKEG-ESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVE 363

Query: 362 DVEYKGYLIPKGWKVMP 379
           DVEY+GYLIPKGWKV+P
Sbjct: 364 DVEYEGYLIPKGWKVLP 369

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0060904.13.1e-193100.00abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa][more]
XP_038884199.18.8e-18890.21abscisic acid 8'-hydroxylase CYP707A1-like [Benincasa hispida][more]
XP_023546528.12.6e-17182.54abscisic acid 8'-hydroxylase 3-like [Cucurbita pepo subsp. pepo][more]
XP_022962256.17.5e-17182.01abscisic acid 8'-hydroxylase 3-like [Cucurbita moschata][more]
XP_022144125.13.2e-16980.63abscisic acid 8'-hydroxylase 4-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A9QNE78.4e-13365.08Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A... [more]
Q9LJK21.5e-12662.17Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 ... [more]
Q0J1852.0e-12666.18Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70... [more]
A2Z2121.3e-12565.90Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
Q09J785.0e-12563.90Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
Match NameE-valueIdentityDescription
A0A5A7V0Q41.5e-193100.00Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A0A0LKV66.8e-18696.19Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357280 PE=3 SV=1[more]
A0A6J1HCL53.6e-17182.01abscisic acid 8'-hydroxylase 3-like OS=Cucurbita moschata OX=3662 GN=LOC11146276... [more]
A0A6J1CQS31.5e-16980.63abscisic acid 8'-hydroxylase 4-like OS=Momordica charantia OX=3673 GN=LOC1110138... [more]
A0A6J1K9Y71.8e-16285.25abscisic acid 8'-hydroxylase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111492434 ... [more]
Match NameE-valueIdentityDescription
AT3G19270.11.1e-12762.17cytochrome P450, family 707, subfamily A, polypeptide 4 [more]
AT4G19230.11.5e-10551.99cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT4G19230.21.5e-10551.99cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT5G45340.14.5e-10551.99cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT5G45340.24.5e-10551.99cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 335..351
score: 32.13
coord: 58..81
score: 23.72
coord: 274..300
score: 28.49
IPR001128Cytochrome P450PFAMPF00067p450coord: 36..381
e-value: 3.8E-47
score: 161.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 26..384
e-value: 4.8E-87
score: 294.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 33..380
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 17..380
NoneNo IPR availablePANTHERPTHR24286:SF103CYTOCHROME P450 FAMILY ABA 8'-HYDROXYLASEcoord: 17..380

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc03g0084341.1Cmc03g0084341.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0010295 (+)-abscisic acid 8'-hydroxylase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen