Cmc03g0069181 (gene) Melon (Charmono) v1.1

Overview
NameCmc03g0069181
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Descriptionflavonol synthase/flavanone 3-hydroxylase
LocationCMiso1.1chr03: 13951021 .. 13952606 (-)
RNA-Seq ExpressionCmc03g0069181
SyntenyCmc03g0069181
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTGCTCTGAAAACACGCACCAAAGTGGAAGTTAGTTAGAACCGTATCTATTGAAAGCAACCTTCTACCTTCTTTATTCTCTTCAATTTTCATTGTATTAATTGAGGTTCTAAATTCATTCTCTCCTCACCAAAAACAAAAATGGATGTTGGGAGAGTCCAGGAAGTTGCTTCATCTCTTCTTTTCTCCGGCACTCTCCCCTCCGAGTTTGTCCGACCAGAGACCGAACAGCCCGGAATCACTACCGTCAAAGGCCAAGTCCTTGCTGTCCCTGTCATTGATTTCACCAACCCAGATGACGATTCTGTTCTCACAGCCATTCACGACGCTTCTCTCAATTGGGGCATGTTTCAAATCATTAACCACCAGATCCCAAATGAAGTAATAACCCATTTGCAAGCCGTCGGGAAGGAATTCTTCGAGCTCCGACCTGAAGAGAAAGAGATATACGCCAAACCCTCTGCTTCCAAATCCGTCGAAGGCTACGGAACTAAGCTCGAAAAAGAAATCGATGGAAAAAGAGGCTGGGTCGACCATTTGTTCCACATCATTTGGCCTCCTTCTTCCATTAATTACAATTTCTGGCCTCAAAATCCCCCTTCTTACAGGTAACGAAATCAATAAATTCCTCCGAGTTCTTCTCTTAATAATTTGTTATTGTACTCTGTTTTGATTTTTGGGTGCACGTGTGTCCGAGAATGCAGGGTAGCGAATGAAGAGTACAGAAAGAATTTACATGGGGTTGTGGAGAAGCTGTTCAGAAGCCTCTCGCTGGGTTTGGGGCTTGAAGGGCATGAACTAAAAGAGCGAGTGGGTGGAGATGAATTGGTGTATTTACTGAAAATCAATTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCAGCCGTTACGATTCTTGTGCCGAATGAAGTTCAAGGACTTCAGGCGTTTAGAGATGGGCATTGGTATGATGTTAATTACATTCCTAATGCTCTTGTCATCCATATCGGAGATCAAATTGAGGTAAAGTGTTTCTAGAATTCAAGAGATGTTTGATTTTTTGAGTTGCGTGGGAAATGGCTGAATTAGAAGTTTGTTGTGTTGTGGGGGCAGATTCTGAGTAATGGAAAGTACAAGAGTGTGTTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCCGTGTTCTTGGAGCCACCGCCGGAGTTTGAAGTTGGGCCTCATCCGAAATTGGTCGATGAGGAAAATCCGGCAAAATATAAGACTAAGAAGTACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCACAGTAACTCAAAACACCGCCGCCGCTTCCTTTAATCGTCTTCGTTTTGTCTGCTCCGGCCGCTGTGTTCTTTGTTCTGCTTACAATGTCTATTTAAATAATAATGAAACTTCCCCTTGTTTATTGAAACTTATTTTGAGAATATAAAACCCAAAAATATCTAAATTAATCTACATATATATGGAACAATAAACAACAATATCCCACGATTATTTGTTTTCATAAGTTACCGATTCACCTTTATTTTTTGGACCAGT

mRNA sequence

TTCTGCTCTGAAAACACGCACCAAAGTGGAAGTTAGTTAGAACCGTATCTATTGAAAGCAACCTTCTACCTTCTTTATTCTCTTCAATTTTCATTGTATTAATTGAGGTTCTAAATTCATTCTCTCCTCACCAAAAACAAAAATGGATGTTGGGAGAGTCCAGGAAGTTGCTTCATCTCTTCTTTTCTCCGGCACTCTCCCCTCCGAGTTTGTCCGACCAGAGACCGAACAGCCCGGAATCACTACCGTCAAAGGCCAAGTCCTTGCTGTCCCTGTCATTGATTTCACCAACCCAGATGACGATTCTGTTCTCACAGCCATTCACGACGCTTCTCTCAATTGGGGCATGTTTCAAATCATTAACCACCAGATCCCAAATGAAGTAATAACCCATTTGCAAGCCGTCGGGAAGGAATTCTTCGAGCTCCGACCTGAAGAGAAAGAGATATACGCCAAACCCTCTGCTTCCAAATCCGTCGAAGGCTACGGAACTAAGCTCGAAAAAGAAATCGATGGAAAAAGAGGCTGGGTCGACCATTTGTTCCACATCATTTGGCCTCCTTCTTCCATTAATTACAATTTCTGGCCTCAAAATCCCCCTTCTTACAGGGTAGCGAATGAAGAGTACAGAAAGAATTTACATGGGGTTGTGGAGAAGCTGTTCAGAAGCCTCTCGCTGGGTTTGGGGCTTGAAGGGCATGAACTAAAAGAGCGAGTGGGTGGAGATGAATTGGTGTATTTACTGAAAATCAATTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCAGCCGTTACGATTCTTGTGCCGAATGAAGTTCAAGGACTTCAGGCGTTTAGAGATGGGCATTGGTATGATGTTAATTACATTCCTAATGCTCTTGTCATCCATATCGGAGATCAAATTGAGATTCTGAGTAATGGAAAGTACAAGAGTGTGTTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCCGTGTTCTTGGAGCCACCGCCGGAGTTTGAAGTTGGGCCTCATCCGAAATTGGTCGATGAGGAAAATCCGGCAAAATATAAGACTAAGAAGTACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCACAGTAACTCAAAACACCGCCGCCGCTTCCTTTAATCGTCTTCGTTTTGTCTGCTCCGGCCGCTGTGTTCTTTGTTCTGCTTACAATGTCTATTTAAATAATAATGAAACTTCCCCTTGTTTATTGAAACTTATTTTGAGAATATAAAACCCAAAAATATCTAAATTAATCTACATATATATGGAACAATAAACAACAATATCCCACGATTATTTGTTTTCATAAGTTACCGATTCACCTTTATTTTTTGGACCAGT

Coding sequence (CDS)

ATGGATGTTGGGAGAGTCCAGGAAGTTGCTTCATCTCTTCTTTTCTCCGGCACTCTCCCCTCCGAGTTTGTCCGACCAGAGACCGAACAGCCCGGAATCACTACCGTCAAAGGCCAAGTCCTTGCTGTCCCTGTCATTGATTTCACCAACCCAGATGACGATTCTGTTCTCACAGCCATTCACGACGCTTCTCTCAATTGGGGCATGTTTCAAATCATTAACCACCAGATCCCAAATGAAGTAATAACCCATTTGCAAGCCGTCGGGAAGGAATTCTTCGAGCTCCGACCTGAAGAGAAAGAGATATACGCCAAACCCTCTGCTTCCAAATCCGTCGAAGGCTACGGAACTAAGCTCGAAAAAGAAATCGATGGAAAAAGAGGCTGGGTCGACCATTTGTTCCACATCATTTGGCCTCCTTCTTCCATTAATTACAATTTCTGGCCTCAAAATCCCCCTTCTTACAGGGTAGCGAATGAAGAGTACAGAAAGAATTTACATGGGGTTGTGGAGAAGCTGTTCAGAAGCCTCTCGCTGGGTTTGGGGCTTGAAGGGCATGAACTAAAAGAGCGAGTGGGTGGAGATGAATTGGTGTATTTACTGAAAATCAATTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCAGCCGTTACGATTCTTGTGCCGAATGAAGTTCAAGGACTTCAGGCGTTTAGAGATGGGCATTGGTATGATGTTAATTACATTCCTAATGCTCTTGTCATCCATATCGGAGATCAAATTGAGATTCTGAGTAATGGAAAGTACAAGAGTGTGTTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCCGTGTTCTTGGAGCCACCGCCGGAGTTTGAAGTTGGGCCTCATCCGAAATTGGTCGATGAGGAAAATCCGGCAAAATATAAGACTAAGAAGTACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCACAGTAA

Protein sequence

MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLEKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ
Homology
BLAST of Cmc03g0069181 vs. NCBI nr
Match: XP_008445671.1 (PREDICTED: flavonol synthase/flavanone 3-hydroxylase [Cucumis melo] >KAA0063921.1 flavonol synthase/flavanone 3-hydroxylase [Cucumis melo var. makuwa] >TYK11311.1 flavonol synthase/flavanone 3-hydroxylase [Cucumis melo var. makuwa])

HSP 1 Score: 691.8 bits (1784), Expect = 2.9e-195
Identity = 331/335 (98.81%), Postives = 331/335 (98.81%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
           HDASLNWGMFQIINHQIPNE ITHLQAVGKEFFEL PEEKEIYAKP ASKSVEGYGTKLE
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 336
            VGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

BLAST of Cmc03g0069181 vs. NCBI nr
Match: XP_004140862.1 (flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN45965.1 hypothetical protein Csa_005329 [Cucumis sativus])

HSP 1 Score: 671.4 bits (1731), Expect = 4.1e-189
Identity = 318/335 (94.93%), Postives = 327/335 (97.61%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQEVASSLLFSGTLPS+FVRP+TEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
           HDASLNWGMFQIINHQIPNE ITHLQAVGKEFF+L PE KEIYAKP ASKSVEGYGT L+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYR ANEEYRK+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSV HRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVDE+ PA+YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEKIPARYKTKKYSEYAYCKLNKIPQ 335

BLAST of Cmc03g0069181 vs. NCBI nr
Match: XP_038885635.1 (flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida])

HSP 1 Score: 644.4 bits (1661), Expect = 5.3e-181
Identity = 302/334 (90.42%), Postives = 319/334 (95.51%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQ++ASS+L+S TLP+EFVR ETEQPGITTVKGQVLAVPVIDFT+PDDDSVL AI
Sbjct: 1   MEVGRVQDIASSVLYSDTLPAEFVRSETEQPGITTVKGQVLAVPVIDFTDPDDDSVLRAI 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
            DAS NWGMFQIINH+IPNE ITHLQAVGKEFFEL PEEKE+YAKP  SKSVEGYGTKL+
Sbjct: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KEIDGK+GWVDHLFHIIWPPS+INYNFWPQNPPSYR ANEEY K+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKKGWVDHLFHIIWPPSAINYNFWPQNPPSYRAANEEYSKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKER GG+ELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERAGGEELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDG WYDVNYIPNALVIHIGDQ+EILSNGKYKSVFHRT VNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGQWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTRVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIP 335
           EVGPHPKLVDE+NP KYKTKKYSEYAYCKLNKIP
Sbjct: 301 EVGPHPKLVDEKNPPKYKTKKYSEYAYCKLNKIP 334

BLAST of Cmc03g0069181 vs. NCBI nr
Match: KAG7016505.1 (Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 622.5 bits (1604), Expect = 2.2e-174
Identity = 287/335 (85.67%), Postives = 312/335 (93.13%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQ++ASS LFSG+LP+EFVRP+TEQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
            DAS NWGMFQIINH IPNE I HLQAVGK FFEL PEEKE+YAKP  SKSVEGYGTKL+
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+IN+ FWPQNPPSYR ANEEYRK+LHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKE  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS +TILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD++NP KYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of Cmc03g0069181 vs. NCBI nr
Match: XP_023551148.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 622.5 bits (1604), Expect = 2.2e-174
Identity = 287/335 (85.67%), Postives = 312/335 (93.13%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQ++ASS LFSG+LP+EFVRP+TEQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIRAI 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
            DAS NWGMFQIINH IPNE I HLQAVGK FFEL PEEKE+YAKP  SKSVEGYGTKL+
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+IN+ FWPQNPPSYR ANEEYRK+LHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKE  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS +TILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD++NP KYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of Cmc03g0069181 vs. ExPASy Swiss-Prot
Match: Q9M547 (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellianum OX=52518 GN=FLS PE=2 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 1.2e-143
Identity = 232/335 (69.25%), Postives = 280/335 (83.58%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+V RVQE+AS      T+P+E++R E EQP I+TV G VL VPVID ++ D+  ++  +
Sbjct: 1   MEVQRVQEIASLSKVIDTIPAEYIRSENEQPVISTVHGVVLEVPVIDLSDSDEKKIVGLV 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
            +AS  WG+FQ++NH IPNEVI  LQ VGK FFEL  EEKE+ AKP  S+S+EGYGT+L+
Sbjct: 61  SEASKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFH IWPPS+INY FWP+NPP+YR ANEEY K L  VV+ LF+ LSLG
Sbjct: 121 KEVDGKKGWVDHLFHKIWPPSAINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           L LE +  K+  GGD+LVYL+KINYYPPCPRPDLALGV AHTDMSA+T+LVPNEV GLQ 
Sbjct: 181 LDLEPNSFKDGAGGDDLVYLMKINYYPPCPRPDLALGV-AHTDMSAITVLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           ++DGHWYD  YIPNAL++HIGDQ+EI+SNGKYKSV+HRTTVNKEKTR+SWPVFLEPPP+ 
Sbjct: 241 YKDGHWYDCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDH 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 336
           EVGP PKLV+EENPAK+KTKKY +YAYCKLNK+PQ
Sbjct: 301 EVGPIPKLVNEENPAKFKTKKYKDYAYCKLNKLPQ 334

BLAST of Cmc03g0069181 vs. ExPASy Swiss-Prot
Match: Q07512 (Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 1.6e-143
Identity = 233/336 (69.35%), Postives = 276/336 (82.14%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+V RVQ +AS      T+PSE++R E EQP  TT+ G VL VPVID  +PD++ ++  I
Sbjct: 13  MEVARVQAIASLSKCMDTIPSEYIRSENEQPAATTLHGVVLQVPVIDLRDPDENKMVKLI 72

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRP-EEKEIYAKPSASKSVEGYGTKL 120
            DAS  WG+FQ+INH IP+E I  LQ VGKEFFE  P EEKE+ AK   S  +EGYGT L
Sbjct: 73  ADASKEWGIFQLINHGIPDEAIADLQKVGKEFFEHVPQEEKELIAKTPGSNDIEGYGTSL 132

Query: 121 EKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSL 180
           +KE++GK+GWVDHLFH IWPPS++NY +WP+NPPSYR ANEEY K +  VV+++F+SLSL
Sbjct: 133 QKEVEGKKGWVDHLFHKIWPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKSLSL 192

Query: 181 GLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQ 240
           GLGLEGHE+ E  GGDE+VYLLKINYYPPCPRPDLALGV AHTDMS +TILVPNEVQGLQ
Sbjct: 193 GLGLEGHEMIEAAGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVPNEVQGLQ 252

Query: 241 AFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPE 300
            F+DGHWYDV YIPNAL++HIGDQ+EILSNGKYKSV+HRTTVNK+KTR+SWPVFLEPP E
Sbjct: 253 VFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPSE 312

Query: 301 FEVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 336
            EVGP PKL+ E NP K+KTKKY +Y YCKLNK+PQ
Sbjct: 313 HEVGPIPKLLSEANPPKFKTKKYKDYVYCKLNKLPQ 348

BLAST of Cmc03g0069181 vs. ExPASy Swiss-Prot
Match: Q9XHG2 (Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 6.6e-142
Identity = 236/323 (73.07%), Postives = 271/323 (83.90%), Query Frame = 0

Query: 17  GTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQ 76
           GT+P+EF+R E EQPGITTV G+VL VP+IDF++PD++ ++  I +AS NWGM+QI+NH 
Sbjct: 15  GTIPAEFIRSENEQPGITTVHGKVLEVPIIDFSDPDEEKLIVQITEASSNWGMYQIVNHD 74

Query: 77  IPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLEKEID----GKRGWVDH 136
           IP+EVI+ LQAVGKEFFEL  EEKE YAKP  S S+EGYGTKL KEI      K+GWVD+
Sbjct: 75  IPSEVISKLQAVGKEFFELPQEEKEAYAKPPDSASIEGYGTKLFKEISEGDTTKKGWVDN 134

Query: 137 LFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLGLGLEGHELKERV 196
           LF+ IWPPS +NY FWP+NPPSYR ANEEY K+LH VVEKLFR LSLGLGLEG ELK+  
Sbjct: 135 LFNKIWPPSVVNYQFWPKNPPSYREANEEYAKHLHNVVEKLFRLLSLGLGLEGQELKKAA 194

Query: 197 GGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQAFRDGHWYDVNYI 256
           GGD L YLLKINYYPPCPRPDLALGV AHTDMS VTILVPN+VQGLQA +DG WYDV YI
Sbjct: 195 GGDNLEYLLKINYYPPCPRPDLALGVVAHTDMSTVTILVPNDVQGLQACKDGRWYDVKYI 254

Query: 257 PNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEE 316
           PNALVIHIGDQ+EI+SNGKY SV HRTTVNK+KTRISWPVFLEPP +  VGPHP+LV+  
Sbjct: 255 PNALVIHIGDQMEIMSNGKYTSVLHRTTVNKDKTRISWPVFLEPPADHVVGPHPQLVNAV 314

Query: 317 NPAKYKTKKYSEYAYCKLNKIPQ 336
           N  KYKTKKY +Y YCK+NK+PQ
Sbjct: 315 NQPKYKTKKYGDYVYCKINKLPQ 337

BLAST of Cmc03g0069181 vs. ExPASy Swiss-Prot
Match: Q7XZQ6 (Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS PE=1 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 3.6e-140
Identity = 227/337 (67.36%), Postives = 276/337 (81.90%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDF--TNPDDDSVLT 60
           M+V RVQ ++       T+PSE++R E+EQP +TT++G VL VPVID   +N  +++++ 
Sbjct: 1   MEVERVQAISKMSRCMDTIPSEYIRSESEQPAVTTMQGVVLQVPVIDLGSSNNTEENLVE 60

Query: 61  AIHDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTK 120
            I +AS  WG+FQ++NH IP++ I  LQ VGKEFFEL  +EKE+ AKP   + VEGYGTK
Sbjct: 61  LIAEASREWGIFQVVNHGIPDDAIAKLQKVGKEFFELPQQEKEVIAKPEGYQGVEGYGTK 120

Query: 121 LEKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLS 180
           L+KE+ GK+GWVDHLFHIIWP S++NYNFWP NPP YR ANEEY   L GVV+KLF +LS
Sbjct: 121 LQKELGGKKGWVDHLFHIIWPKSAVNYNFWPNNPPLYREANEEYAVALRGVVDKLFEALS 180

Query: 181 LGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGL 240
           LG+GLE HELK+  GGD+L+Y+LKINYYPPCPRPDLALGV AHTDMSA+TILVPNEVQGL
Sbjct: 181 LGIGLEKHELKKASGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVPNEVQGL 240

Query: 241 QAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPP 300
           Q  +D HWYDV YIPNAL+IHIGDQIEI+SNGKYKSV+HRTTVNK+KTR+SWPVFLEPPP
Sbjct: 241 QVHKDDHWYDVKYIPNALIIHIGDQIEIMSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPP 300

Query: 301 EFEVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 336
           E   GP  KL+ +ENPAK+KTKKY +Y YCKLNK+PQ
Sbjct: 301 ELLTGPISKLITDENPAKFKTKKYKDYVYCKLNKLPQ 337

BLAST of Cmc03g0069181 vs. ExPASy Swiss-Prot
Match: Q9ZWQ9 (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 1.1e-136
Identity = 221/335 (65.97%), Postives = 270/335 (80.60%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+V RVQ +AS    +GT+P+EF+RPE EQP  TT  G    +P ID  +P  D ++ +I
Sbjct: 1   MEVERVQAIASLSHSNGTIPAEFIRPEKEQPASTTYHGPAPEIPTIDLDDPVQDRLVRSI 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
            +AS  WG+FQ+ NH IP+++I  LQAVGKEFFEL  EEKE+Y++P+ +K V+GYGTKL+
Sbjct: 61  AEASREWGIFQVTNHGIPSDLICKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KE++GK+ WVDHLFH +WPPSSINY FWP+NPPSYR  NEEY K +  VV+KLF  LSLG
Sbjct: 121 KEVEGKKSWVDHLFHRVWPPSSINYRFWPKNPPSYRAVNEEYAKYMREVVDKLFTYLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LG+EG  LKE  GGD++ Y+LKINYYPPCPRPDLALGV AHTD+SA+T+LVPNEV GLQ 
Sbjct: 181 LGVEGGVLKEAAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           F+D  W D  YIPNALVIHIGDQIEILSNGKYK+V HRTTVNK+KTR+SWPVFLEPP + 
Sbjct: 241 FKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPPADT 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 336
            VGP P+LVD+ENP KYK KK+ +Y+YCKLNK+PQ
Sbjct: 301 VVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ 335

BLAST of Cmc03g0069181 vs. ExPASy TrEMBL
Match: A0A5D3CHN5 (Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1827G00220 PE=3 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 1.4e-195
Identity = 331/335 (98.81%), Postives = 331/335 (98.81%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
           HDASLNWGMFQIINHQIPNE ITHLQAVGKEFFEL PEEKEIYAKP ASKSVEGYGTKLE
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 336
            VGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

BLAST of Cmc03g0069181 vs. ExPASy TrEMBL
Match: A0A1S3BCS7 (flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC103488630 PE=3 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 1.4e-195
Identity = 331/335 (98.81%), Postives = 331/335 (98.81%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
           HDASLNWGMFQIINHQIPNE ITHLQAVGKEFFEL PEEKEIYAKP ASKSVEGYGTKLE
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 336
            VGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

BLAST of Cmc03g0069181 vs. ExPASy TrEMBL
Match: A0A0A0K885 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040540 PE=3 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 2.0e-189
Identity = 318/335 (94.93%), Postives = 327/335 (97.61%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQEVASSLLFSGTLPS+FVRP+TEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
           HDASLNWGMFQIINHQIPNE ITHLQAVGKEFF+L PE KEIYAKP ASKSVEGYGT L+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYR ANEEYRK+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSV HRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVDE+ PA+YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEKIPARYKTKKYSEYAYCKLNKIPQ 335

BLAST of Cmc03g0069181 vs. ExPASy TrEMBL
Match: A0A6J1FDW3 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111444648 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.3e-174
Identity = 286/335 (85.37%), Postives = 312/335 (93.13%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQ++ASS LFSG+LP+EFVRP+TEQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
            DAS NWGMFQIINH IPNE I HLQAVGK FFEL PEEKE+YAKP  SKSVEGYGTKL+
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KE+DG++GWVDHLFHIIWPPS+IN+ FWPQNPPSYR ANEEYRK+LHGVV+KLFRSLSLG
Sbjct: 121 KEVDGRKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKE  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS +TILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD++NP KYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of Cmc03g0069181 vs. ExPASy TrEMBL
Match: A0A6J1JY72 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111489355 PE=3 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 6.8e-174
Identity = 285/335 (85.07%), Postives = 312/335 (93.13%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQ++ASS LFSG++P+EFVRP+ EQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSIPAEFVRPDPEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
            DAS NWGMFQIINH IPNE I HLQAVGK FFEL PEEKE+YAKP  SKSVEGYGTKL+
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+IN+ FWPQNPPSYRVANEEYRK+LHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRVANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELK+  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS +TILVPN+VQGLQA
Sbjct: 181 LGLEGHELKQSAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRARISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD++NP KYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of Cmc03g0069181 vs. TAIR 10
Match: AT5G08640.1 (flavonol synthase 1 )

HSP 1 Score: 438.0 bits (1125), Expect = 7.0e-123
Identity = 198/334 (59.28%), Postives = 255/334 (76.35%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+V RVQ+++SS L +  +P EF+R E EQP ITT +G   A+PV+D ++PD++SV  A+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
             AS  WG+FQ++NH IP E+I  LQ VG++FFEL   EKE  AKP  SK +EGYGTKL+
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           K+ +GK+ WVDHLFH IWPPS +NY FWP+NPP YR  NEEY  ++  + E L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGL+   LKE +GG+   Y++KINYYPPCPRPDLALGVPAHTD+S +T+LVPNEV GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           F+D HW+D  YIP+A+++HIGDQI  LSNG+YK+V HRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIP 335
            VGP P+L  ++NP K+K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

BLAST of Cmc03g0069181 vs. TAIR 10
Match: AT5G08640.2 (flavonol synthase 1 )

HSP 1 Score: 438.0 bits (1125), Expect = 7.0e-123
Identity = 198/334 (59.28%), Postives = 255/334 (76.35%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+V RVQ+++SS L +  +P EF+R E EQP ITT +G   A+PV+D ++PD++SV  A+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  HDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKSVEGYGTKLE 120
             AS  WG+FQ++NH IP E+I  LQ VG++FFEL   EKE  AKP  SK +EGYGTKL+
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           K+ +GK+ WVDHLFH IWPPS +NY FWP+NPP YR  NEEY  ++  + E L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGL+   LKE +GG+   Y++KINYYPPCPRPDLALGVPAHTD+S +T+LVPNEV GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           F+D HW+D  YIP+A+++HIGDQI  LSNG+YK+V HRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIP 335
            VGP P+L  ++NP K+K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

BLAST of Cmc03g0069181 vs. TAIR 10
Match: AT5G63590.1 (flavonol synthase 3 )

HSP 1 Score: 332.0 bits (850), Expect = 5.4e-91
Identity = 152/294 (51.70%), Postives = 202/294 (68.71%), Query Frame = 0

Query: 41  LAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEK 100
           L +PVID +NPD++ V +A+  AS  WG+FQ++NH IP E+I  L  VG EFFEL   EK
Sbjct: 12  LDIPVIDLSNPDEELVASAVVKASQEWGIFQVVNHGIPTELILRLLQVGMEFFELPETEK 71

Query: 101 EIYAKPSASKSVEGYGTKLEKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANE 160
           E  AKP  S  +EGY TK +K+++G+  WVDHLFH IWPPS +N+ FWP+NPP Y   NE
Sbjct: 72  EAVAKPEDSLDIEGYRTKYQKDLEGRNAWVDHLFHRIWPPSRVNHKFWPKNPPEYIEVNE 131

Query: 161 EYRKNLHGVVEKLFRSLSLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPA 220
           EY  ++  + EK+   LS GLGL    LKE +GG+ + YL+KINYYPPCP P+L +G P 
Sbjct: 132 EYASHIKKLSEKIMEWLSEGLGLRHEALKEGLGGETIEYLMKINYYPPCPDPELVVGAPD 191

Query: 221 HTDMSAVTILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTT 280
           HTD++ +T+LV NE  GLQAF+D  W D  Y  + +++ IGDQ   +SNGKYKSV HR  
Sbjct: 192 HTDVNGITLLVANEALGLQAFKDNQWIDAEYTTSGIIVIIGDQFLRMSNGKYKSVEHRAK 251

Query: 281 VNKEKTRISWPVFLEPPPEFEVGPHPKLV-DEENPAKYKTKKYSEYAYCKLNKI 334
           ++KEKTRISWPVF+E   +   GP P+L+  +EN  K+K   Y +Y + KL K+
Sbjct: 252 MDKEKTRISWPVFVESSLDQVFGPLPELITGDENVPKFKPYVYKDYKFRKLKKL 305

BLAST of Cmc03g0069181 vs. TAIR 10
Match: AT4G22880.1 (leucoanthocyanidin dioxygenase )

HSP 1 Score: 289.3 bits (739), Expect = 4.0e-78
Identity = 147/341 (43.11%), Postives = 213/341 (62.46%), Query Frame = 0

Query: 3   VGRVQEVASSLLFSGTLPSEFVRPETEQPGITTV-----KGQVLAVPVIDFTNPDDD--- 62
           V RV+ +A S + S  +P E++RP+ E   I  V     K     VP ID  N + D   
Sbjct: 4   VERVESLAKSGIIS--IPKEYIRPKEELESINDVFLEEKKEDGPQVPTIDLKNIESDDEK 63

Query: 63  ---SVLTAIHDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKS 122
              + +  +  ASL+WG+  +INH IP +++  ++  G+EFF L  EEKE YA   A+  
Sbjct: 64  IRENCIEELKKASLDWGVMHLINHGIPADLMERVKKAGEEFFSLSVEEKEKYANDQATGK 123

Query: 123 VEGYGTKLEKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVE 182
           ++GYG+KL     G+  W D+ FH+ +P    + + WP+ P  Y  A  EY K L  +  
Sbjct: 124 IQGYGSKLANNASGQLEWEDYFFHLAYPEEKRDLSIWPKTPSDYIEATSEYAKCLRLLAT 183

Query: 183 KLFRSLSLGLGLEGHELKERVGG-DELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTIL 242
           K+F++LS+GLGLE   L++ VGG +EL+  +KINYYP CP+P+LALGV AHTD+SA+T +
Sbjct: 184 KVFKALSVGLGLEPDRLEKEVGGLEELLLQMKINYYPKCPQPELALGVEAHTDVSALTFI 243

Query: 243 VPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISW 302
           + N V GLQ F +G W     +P+++V+HIGD +EILSNGKYKS+ HR  VNKEK RISW
Sbjct: 244 LHNMVPGLQLFYEGKWVTAKCVPDSIVMHIGDTLEILSNGKYKSILHRGLVNKEKVRISW 303

Query: 303 PVFLEPPPE-FEVGPHPKLVDEENPAKYKTKKYSEYAYCKL 331
            VF EPP +   + P P++V  E+PAK+  + ++++   KL
Sbjct: 304 AVFCEPPKDKIVLKPLPEMVSVESPAKFPPRTFAQHIEHKL 342

BLAST of Cmc03g0069181 vs. TAIR 10
Match: AT4G22880.2 (leucoanthocyanidin dioxygenase )

HSP 1 Score: 289.3 bits (739), Expect = 4.0e-78
Identity = 147/341 (43.11%), Postives = 213/341 (62.46%), Query Frame = 0

Query: 3   VGRVQEVASSLLFSGTLPSEFVRPETEQPGITTV-----KGQVLAVPVIDFTNPDDD--- 62
           V RV+ +A S + S  +P E++RP+ E   I  V     K     VP ID  N + D   
Sbjct: 4   VERVESLAKSGIIS--IPKEYIRPKEELESINDVFLEEKKEDGPQVPTIDLKNIESDDEK 63

Query: 63  ---SVLTAIHDASLNWGMFQIINHQIPNEVITHLQAVGKEFFELRPEEKEIYAKPSASKS 122
              + +  +  ASL+WG+  +INH IP +++  ++  G+EFF L  EEKE YA   A+  
Sbjct: 64  IRENCIEELKKASLDWGVMHLINHGIPADLMERVKKAGEEFFSLSVEEKEKYANDQATGK 123

Query: 123 VEGYGTKLEKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVE 182
           ++GYG+KL     G+  W D+ FH+ +P    + + WP+ P  Y  A  EY K L  +  
Sbjct: 124 IQGYGSKLANNASGQLEWEDYFFHLAYPEEKRDLSIWPKTPSDYIEATSEYAKCLRLLAT 183

Query: 183 KLFRSLSLGLGLEGHELKERVGG-DELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTIL 242
           K+F++LS+GLGLE   L++ VGG +EL+  +KINYYP CP+P+LALGV AHTD+SA+T +
Sbjct: 184 KVFKALSVGLGLEPDRLEKEVGGLEELLLQMKINYYPKCPQPELALGVEAHTDVSALTFI 243

Query: 243 VPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISW 302
           + N V GLQ F +G W     +P+++V+HIGD +EILSNGKYKS+ HR  VNKEK RISW
Sbjct: 244 LHNMVPGLQLFYEGKWVTAKCVPDSIVMHIGDTLEILSNGKYKSILHRGLVNKEKVRISW 303

Query: 303 PVFLEPPPE-FEVGPHPKLVDEENPAKYKTKKYSEYAYCKL 331
            VF EPP +   + P P++V  E+PAK+  + ++++   KL
Sbjct: 304 AVFCEPPKDKIVLKPLPEMVSVESPAKFPPRTFAQHIEHKL 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008445671.12.9e-19598.81PREDICTED: flavonol synthase/flavanone 3-hydroxylase [Cucumis melo] >KAA0063921.... [more]
XP_004140862.14.1e-18994.93flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN45965.1 hypothet... [more]
XP_038885635.15.3e-18190.42flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida][more]
KAG7016505.12.2e-17485.67Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp... [more]
XP_023551148.12.2e-17485.67flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9M5471.2e-14369.25Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellian... [more]
Q075121.6e-14369.35Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 ... [more]
Q9XHG26.6e-14273.07Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2... [more]
Q7XZQ63.6e-14067.36Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS... [more]
Q9ZWQ91.1e-13665.97Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A5D3CHN51.4e-19598.81Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BCS71.4e-19598.81flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC10348863... [more]
A0A0A0K8852.0e-18994.93Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G... [more]
A0A6J1FDW32.3e-17485.37flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JY726.8e-17485.07flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G08640.17.0e-12359.28flavonol synthase 1 [more]
AT5G08640.27.0e-12359.28flavonol synthase 1 [more]
AT5G63590.15.4e-9151.70flavonol synthase 3 [more]
AT4G22880.14.0e-7843.11leucoanthocyanidin dioxygenase [more]
AT4G22880.24.0e-7843.11leucoanthocyanidin dioxygenase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 201..296
e-value: 2.6E-25
score: 88.8
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..333
e-value: 2.8E-95
score: 321.4
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 43..150
e-value: 3.6E-21
score: 76.1
NoneNo IPR availablePANTHERPTHR47990:SF36OS02G0767300 PROTEINcoord: 6..332
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 6..332
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 17..333
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 196..296
score: 14.531129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc03g0069181.1Cmc03g0069181.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051555 flavonol biosynthetic process
biological_process GO:0009416 response to light stimulus
molecular_function GO:0045431 flavonol synthase activity
molecular_function GO:0046872 metal ion binding