Cmc02g0058361 (gene) Melon (Charmono) v1.1

Overview
NameCmc02g0058361
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionKinetochore protein SPC25
LocationCMiso1.1chr02: 23926975 .. 23929667 (+)
RNA-Seq ExpressionCmc02g0058361
SyntenyCmc02g0058361
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATATTATCTCTAGAGAAGAAAATTTAATTTTGAAGGTTAAAGCAAATTTGGCGGGCGAACGAGTGTACAGCAACTCCTTTGCTCTGTGGGAGAATGGAGATCAAGGCGACTGATTCTGTTCGTGCGAGGTTGGAAAAACTTCGAATCGTTCGTGAAAATGAAATTCCAGTTCAGCAGCAAAAGATGGAGTCCTTCGCGGATTCATTTTGCAAATCAGTGGAATCGATCAGTGTAAGGTCCCGAGCAAATGCCCTAAGTCAAGGTGAGCTATCTATTTTCTTCATTTTATTTGGGAAAGAAATGGGTGTGAAACTTGTTACTGTGGCCACTTACGACACTGTAATCGATATTAGAATGGTTTGTGAAGTGACCATTAGGGTTTTGAAATGGGTAGCGATTAAGACTCAGAATGGATGTAATTCTAGGTAATTGAGTTGGAGTTGGTTCAGAAGTGGCGAAGTTTACTTTGAAATTTCTTGTTTCGTTTTTTTGTTTTGTTTTTGTTTATATATATATTTTTAATTCTCAGGGAAACTGGGGGAACTAAAAGCGAGGCTGAGGGTGGCGGAAGATGAACTGGTTAAAACTCTTGCAGGTATGAGCTATGATTATGCTACTTTCCTTCACCGCTGTATGAGTTAGAATATACTACTTCATTGAAGATTGAATCGACTTTAAGTGGCATTTAAGAGTTGAGTTATTTTTCCACTAAATTGTCGAGGATCTTTTAACGGTAGTGTTTTGGATTTGGAGCGAGTGTTGGGCGAAATGTTGGTGTGTGTGATGCTGGTATATTGTTTCTTACTTAAAGTTATTGCTCGTCGTATGGGCTTATTTCCTTTATACTTGCATATTTTTGTAGTGAAGACTCGTAAAGAGGCCAAACGCCTCACAATCATCGACTCCATTGCAACTTCAAAATCCAGAATAGAAGAACTCAGGAATACTTTACAAGACTGCAAAGCAAGGAGGGACGAATATGCTACAATTATATCTCAGCAATCCTTAGGTAATGTCTGTAAATCTTATTTTCTTTCAGACCCAATTTTCCTTTATGCAAATAGAAAACTGCACATGAACTGAAGGTAAGTTCATTGTTAACAAGTTGGATATGCATTGTTCAATCTTTATTTCCGCAATTCTTTTGCAAACTTCAACTTTAGAAGTTCCATGCGATAACTGATTACTTCTCTTTCTTTCCAATGCTATCAATAGTTTTATCTTCATCTGAACGCAAGGAAACCCAGGAGAGCGAGTGCAGAAATGAAATACAAGAGGCTATTTCTTGGTACAAAAGGGTTCTTGATTTTCATATTGAAGGAGGATATGGTATGATAATTCAAGTTTGTTTATGAAGAATCTCAACGGTTTTTGGGAAGCTTGAGATAAATTTGTTGACAACTTCTCTCGGCATTTCAGGGGTGAAATTCTCTTTCAAGAAAATAAATATAGACAATCCTGATGAAGAATATTCTTTTACGATCCGTCATGCAAATGATACTTATATGTGTAAGTTTTATCATATGTGCACTTAATATATGTTGATTTTTCTTGCTCATTATCTTCTGACAACCTGTATTTTTTTCTCTTGACTTTTTAGTGTTAGATTGTAACCCATCCTTGCCAGATATTAAGGAGTTGATACATGAATTAAATAAAACAAATGGTTTGTTCAAGCTCGTCAGGATCATGAGACGGAGATTCCAGGAGGCTGCAGCAGAAGGTGTGCCCTGAATTTTTAACTTTTTGCTTGAAAGGTATTTTTCCACTAAACGGTTGTTATATGTTTGCTATTTTCAACAGGATCTGGGGCTCCAGCATTGTCTCTGCATCAAGGTTCATCAATAATATCTACGTCAGCACCAGGCTTGTCATCATCAACTGAGAGAAGTGATTCTTCAACTGAAGAAATTAACATGGATGAAGTTGAGGAAACTAACAAACATTCTAAGAACATTTTACCCAGAAAGAAACCAGCTATCCACTCCAGTGGATCTGCTTTTTCTTTGCGTAGATCTCCTCGTTTTAAGGTATATTCAATTTTAATGTTTGATATCTGCTGCTTTTGATTTAGGATTCTTGAGTATATGATACTAAGAGAATACTATAAGTCTATAACTTCTATGTATAACAGGTTGATCTTTCTTTAAAGCAGATTTCAAATTGTTGTGCAAAAGAGAATTACTAATAAAATGCACCTCGTATGGCAGGTCAGGAAATAAGATTGGAGGCTGTTAATGATTGAACAAGTATTAGGTTGATGGGCTCAGCCTTTTGCTTCCAAATTGCTTTGCAACTAACGTTGATTATGCCATTATGGGTCCTGATCTCTACATTTGCCAGAATTTCTCTGACTACTCTGAAGGGTCCATATCATACAAGTGAAGTTGAAGAAGGCTTTATCACATGACCTGTTGTATGTCCATGTATCTGATTTGTTTTTATGTGTATTATTTTACACGAAGTTATGGTTTTCATGTAATGCATGGGGGTGATTATCCCAAATGTACCGAATGCCAGTACTTTGTATTAATCTGATCTATATTCTAGGTTTCACATCATCGTACTTAAATTAAGGTTTGTGCAGTTTTAAATTAATTAATTGTGAAAGCTTGAAGGTGAAAGTTCACTTCTCTGTGAAATTAAGTTAGTATATAACGATGTAGTTTACATTCTTTAATTAGTAGGTTCT

mRNA sequence

GATATTATCTCTAGAGAAGAAAATTTAATTTTGAAGGTTAAAGCAAATTTGGCGGGCGAACGAGTGTACAGCAACTCCTTTGCTCTGTGGGAGAATGGAGATCAAGGCGACTGATTCTGTTCGTGCGAGGTTGGAAAAACTTCGAATCGTTCGTGAAAATGAAATTCCAGTTCAGCAGCAAAAGATGGAGTCCTTCGCGGATTCATTTTGCAAATCAGTGGAATCGATCAGTGTAAGGTCCCGAGCAAATGCCCTAAGTCAAGGGAAACTGGGGGAACTAAAAGCGAGGCTGAGGGTGGCGGAAGATGAACTGGTTAAAACTCTTGCAGTGAAGACTCGTAAAGAGGCCAAACGCCTCACAATCATCGACTCCATTGCAACTTCAAAATCCAGAATAGAAGAACTCAGGAATACTTTACAAGACTGCAAAGCAAGGAGGGACGAATATGCTACAATTATATCTCAGCAATCCTTAGTTTTATCTTCATCTGAACGCAAGGAAACCCAGGAGAGCGAGTGCAGAAATGAAATACAAGAGGCTATTTCTTGGTACAAAAGGGTTCTTGATTTTCATATTGAAGGAGGATATGGGGTGAAATTCTCTTTCAAGAAAATAAATATAGACAATCCTGATGAAGAATATTCTTTTACGATCCGTCATGCAAATGATACTTATATGTTGTTAGATTGTAACCCATCCTTGCCAGATATTAAGGAGTTGATACATGAATTAAATAAAACAAATGGTTTGTTCAAGCTCGTCAGGATCATGAGACGGAGATTCCAGGAGGCTGCAGCAGAAGGATCTGGGGCTCCAGCATTGTCTCTGCATCAAGGTTCATCAATAATATCTACGTCAGCACCAGGCTTGTCATCATCAACTGAGAGAAGTGATTCTTCAACTGAAGAAATTAACATGGATGAAGTTGAGGAAACTAACAAACATTCTAAGAACATTTTACCCAGAAAGAAACCAGCTATCCACTCCAGTGGATCTGCTTTTTCTTTGCGTAGATCTCCTCGTTTTAAGGTCAGGAAATAAGATTGGAGGCTGTTAATGATTGAACAAGTATTAGGTTGATGGGCTCAGCCTTTTGCTTCCAAATTGCTTTGCAACTAACGTTGATTATGCCATTATGGGTCCTGATCTCTACATTTGCCAGAATTTCTCTGACTACTCTGAAGGGTCCATATCATACAAGTGAAGTTGAAGAAGGCTTTATCACATGACCTGTTGTATGTCCATGTATCTGATTTGTTTTTATGTGTATTATTTTACACGAAGTTATGGTTTTCATGTAATGCATGGGGGTGATTATCCCAAATGTACCGAATGCCAGTACTTTGTATTAATCTGATCTATATTCTAGGTTTCACATCATCGTACTTAAATTAAGGTTTGTGCAGTTTTAAATTAATTAATTGTGAAAGCTTGAAGGTGAAAGTTCACTTCTCTGTGAAATTAAGTTAGTATATAACGATGTAGTTTACATTCTTTAATTAGTAGGTTCT

Coding sequence (CDS)

ATGGAGATCAAGGCGACTGATTCTGTTCGTGCGAGGTTGGAAAAACTTCGAATCGTTCGTGAAAATGAAATTCCAGTTCAGCAGCAAAAGATGGAGTCCTTCGCGGATTCATTTTGCAAATCAGTGGAATCGATCAGTGTAAGGTCCCGAGCAAATGCCCTAAGTCAAGGGAAACTGGGGGAACTAAAAGCGAGGCTGAGGGTGGCGGAAGATGAACTGGTTAAAACTCTTGCAGTGAAGACTCGTAAAGAGGCCAAACGCCTCACAATCATCGACTCCATTGCAACTTCAAAATCCAGAATAGAAGAACTCAGGAATACTTTACAAGACTGCAAAGCAAGGAGGGACGAATATGCTACAATTATATCTCAGCAATCCTTAGTTTTATCTTCATCTGAACGCAAGGAAACCCAGGAGAGCGAGTGCAGAAATGAAATACAAGAGGCTATTTCTTGGTACAAAAGGGTTCTTGATTTTCATATTGAAGGAGGATATGGGGTGAAATTCTCTTTCAAGAAAATAAATATAGACAATCCTGATGAAGAATATTCTTTTACGATCCGTCATGCAAATGATACTTATATGTTGTTAGATTGTAACCCATCCTTGCCAGATATTAAGGAGTTGATACATGAATTAAATAAAACAAATGGTTTGTTCAAGCTCGTCAGGATCATGAGACGGAGATTCCAGGAGGCTGCAGCAGAAGGATCTGGGGCTCCAGCATTGTCTCTGCATCAAGGTTCATCAATAATATCTACGTCAGCACCAGGCTTGTCATCATCAACTGAGAGAAGTGATTCTTCAACTGAAGAAATTAACATGGATGAAGTTGAGGAAACTAACAAACATTCTAAGAACATTTTACCCAGAAAGAAACCAGCTATCCACTCCAGTGGATCTGCTTTTTCTTTGCGTAGATCTCCTCGTTTTAAGGTCAGGAAATAA

Protein sequence

MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLGELKARLRVAEDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSPRFKVRK
Homology
BLAST of Cmc02g0058361 vs. NCBI nr
Match: XP_008464538.1 (PREDICTED: probable kinetochore protein SPC25 [Cucumis melo])

HSP 1 Score: 584.7 bits (1506), Expect = 4.7e-163
Identity = 314/315 (99.68%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60
           MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60

Query: 61  ELKARLRVAEDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
           ELKARLRVAEDELVK LAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT
Sbjct: 61  ELKARLRVAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120

Query: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240
           EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240

Query: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG 300
           PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG
Sbjct: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG 300

Query: 301 SAFSLRRSPRFKVRK 316
           SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315

BLAST of Cmc02g0058361 vs. NCBI nr
Match: KAA0057836.1 (putative kinetochore protein SPC25 [Cucumis melo var. makuwa])

HSP 1 Score: 559.3 bits (1440), Expect = 2.1e-155
Identity = 313/364 (85.99%), Postives = 313/364 (85.99%), Query Frame = 0

Query: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQG--- 60
           MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQG   
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60

Query: 61  ------------------------------------------------KLGELKARLRVA 120
                                                           KLGELKARLRVA
Sbjct: 61  IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120

Query: 121 EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180
           EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL
Sbjct: 121 EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180

Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
           SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240

Query: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300
           ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300

Query: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 314
           SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360

BLAST of Cmc02g0058361 vs. NCBI nr
Match: TYJ98520.1 (putative kinetochore protein SPC25 [Cucumis melo var. makuwa])

HSP 1 Score: 557.4 bits (1435), Expect = 8.0e-155
Identity = 312/364 (85.71%), Postives = 312/364 (85.71%), Query Frame = 0

Query: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQG--- 60
           MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQG   
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60

Query: 61  ------------------------------------------------KLGELKARLRVA 120
                                                           KLGELKARLRVA
Sbjct: 61  IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120

Query: 121 EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180
           EDELVK LAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL
Sbjct: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180

Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
           SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240

Query: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300
           ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300

Query: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 314
           SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360

BLAST of Cmc02g0058361 vs. NCBI nr
Match: XP_004138099.1 (kinetochore protein SPC25 homolog [Cucumis sativus] >KGN63556.1 hypothetical protein Csa_014306 [Cucumis sativus])

HSP 1 Score: 553.1 bits (1424), Expect = 1.5e-153
Identity = 299/315 (94.92%), Postives = 304/315 (96.51%), Query Frame = 0

Query: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60
           ME KA DSVRARLEKLRI+RENEIPVQQQKMESFADSF KSVESISVRSRANALSQGKLG
Sbjct: 1   MENKANDSVRARLEKLRILRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60

Query: 61  ELKARLRVAEDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
           ELKARLR  EDELVK LAVKTRKEAKRLTIIDSIATSKSR EELRNTLQDCKARRDEYAT
Sbjct: 61  ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120

Query: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240
           EEYSFTIRHANDTYMLLDCNPSL DIKELIHELNKTNGLFKLVRIMRRRFQEAAAEG+GA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240

Query: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG 300
           PALSLHQGSS+ISTSAP LSSSTE S+SSTEEIN+DEVEETNKHSKNILPRK PAIHSSG
Sbjct: 241 PALSLHQGSSVISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG 300

Query: 301 SAFSLRRSPRFKVRK 316
           SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315

BLAST of Cmc02g0058361 vs. NCBI nr
Match: XP_038880541.1 (kinetochore protein SPC25 homolog [Benincasa hispida])

HSP 1 Score: 508.1 bits (1307), Expect = 5.6e-140
Identity = 280/316 (88.61%), Postives = 292/316 (92.41%), Query Frame = 0

Query: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60
           ME KA DSVRARLEKLRIVRE EIP QQQKMESF +SF KSVESI+VRSRANALSQGKLG
Sbjct: 1   MENKAKDSVRARLEKLRIVREKEIPFQQQKMESFVNSFYKSVESINVRSRANALSQGKLG 60

Query: 61  ELKARLRVAEDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
           ELKARLR AEDELVK LAVKTRKEAKRLTIIDS+ATSKSRIEELRNTLQD KARRDEY+T
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLTIIDSLATSKSRIEELRNTLQDSKARRDEYST 120

Query: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IIS+QSL LSSSERKE QESECR EIQEAISWYKRVLDFHIEGG+GVKFSFKKIN+ NPD
Sbjct: 121 IISRQSLALSSSERKEPQESECRKEIQEAISWYKRVLDFHIEGGHGVKFSFKKINVVNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240
           EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAA+G  A
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAKGVEA 240

Query: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEIN-MDEVEETNKHSKNILPRKKPAIHSS 300
            ALSLHQGS II  SAPGLSSSTERS+SS+EEIN  DE+EETNKH+K ILP KKPAI SS
Sbjct: 241 QALSLHQGSMIIPMSAPGLSSSTERSESSSEEINKKDELEETNKHTKKILPGKKPAIKSS 300

Query: 301 GSAFSLRRSPRFKVRK 316
           GSAFSLRRSPRFKVRK
Sbjct: 301 GSAFSLRRSPRFKVRK 316

BLAST of Cmc02g0058361 vs. ExPASy Swiss-Prot
Match: Q93VK9 (Kinetochore protein SPC25 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC25 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.2e-49
Identity = 131/314 (41.72%), Postives = 191/314 (60.83%), Query Frame = 0

Query: 3   IKATDSVRARLEKLRIVRENEIPVQQQKMESF-ADSFCKSVESISVRSRANALSQGKLGE 62
           I   D+ +  +  L ++ E +I  Q+ K++SF A  F +S+ S+  R++A A SQ +L  
Sbjct: 7   IAGGDTTKETMASLGLICEKDIHEQRLKIDSFIASPFRRSMNSLVERAQATAQSQVELMN 66

Query: 63  LKARLRVAEDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATI 122
           LKA LR AEDELVK LAVKTRKEA+++ I DSI+ ++SRIE LR  LQ  K+++D+   I
Sbjct: 67  LKADLREAEDELVKVLAVKTRKEARQMGIRDSISATQSRIEVLRRNLQLQKSKKDDSVRI 126

Query: 123 ISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDE 182
           ISQQ   LS S+    + +E + +I EAISWY   L FH+E G+GVKF+F  I+   P  
Sbjct: 127 ISQQLQALSKSKDNAGKVTEDKADIHEAISWYNHALGFHVEAGHGVKFTFTNIDAKRPTR 186

Query: 183 EYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAP 242
           E+SFT+ + ND Y LLD +  L  I E++ ELNKTN LF+ VR+MR +F ++        
Sbjct: 187 EFSFTVHYGNDIYTLLDSDLQLDYINEMVQELNKTNDLFRFVRLMREQFLKSTLSELPTH 246

Query: 243 ALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGS 302
           +  L Q +S IS SAP +S ST+ ++ ST E    +V+   +  +      +  + +  S
Sbjct: 247 SGQLQQETSAISASAPAISFSTD-TNMSTPENKRSKVQVNRRQKRG----SESPLLAPVS 306

Query: 303 AFSLRRSPRFKVRK 316
             + RRS RFK +K
Sbjct: 307 TSATRRSSRFKGKK 315

BLAST of Cmc02g0058361 vs. ExPASy TrEMBL
Match: A0A1S3CM71 (Kinetochore protein SPC25 OS=Cucumis melo OX=3656 GN=LOC103502389 PE=3 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.3e-163
Identity = 314/315 (99.68%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60
           MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60

Query: 61  ELKARLRVAEDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
           ELKARLRVAEDELVK LAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT
Sbjct: 61  ELKARLRVAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120

Query: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240
           EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240

Query: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG 300
           PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG
Sbjct: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG 300

Query: 301 SAFSLRRSPRFKVRK 316
           SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315

BLAST of Cmc02g0058361 vs. ExPASy TrEMBL
Match: A0A5A7UUK0 (Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001230 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.0e-155
Identity = 313/364 (85.99%), Postives = 313/364 (85.99%), Query Frame = 0

Query: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQG--- 60
           MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQG   
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60

Query: 61  ------------------------------------------------KLGELKARLRVA 120
                                                           KLGELKARLRVA
Sbjct: 61  IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120

Query: 121 EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180
           EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL
Sbjct: 121 EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180

Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
           SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240

Query: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300
           ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300

Query: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 314
           SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360

BLAST of Cmc02g0058361 vs. ExPASy TrEMBL
Match: A0A5D3BFE9 (Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001250 PE=3 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.9e-155
Identity = 312/364 (85.71%), Postives = 312/364 (85.71%), Query Frame = 0

Query: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQG--- 60
           MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQG   
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60

Query: 61  ------------------------------------------------KLGELKARLRVA 120
                                                           KLGELKARLRVA
Sbjct: 61  IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120

Query: 121 EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180
           EDELVK LAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL
Sbjct: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180

Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
           SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240

Query: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300
           ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300

Query: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 314
           SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360

BLAST of Cmc02g0058361 vs. ExPASy TrEMBL
Match: A0A0A0LU84 (Kinetochore protein SPC25 OS=Cucumis sativus OX=3659 GN=Csa_1G004230 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 7.3e-154
Identity = 299/315 (94.92%), Postives = 304/315 (96.51%), Query Frame = 0

Query: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60
           ME KA DSVRARLEKLRI+RENEIPVQQQKMESFADSF KSVESISVRSRANALSQGKLG
Sbjct: 1   MENKANDSVRARLEKLRILRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60

Query: 61  ELKARLRVAEDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
           ELKARLR  EDELVK LAVKTRKEAKRLTIIDSIATSKSR EELRNTLQDCKARRDEYAT
Sbjct: 61  ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120

Query: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240
           EEYSFTIRHANDTYMLLDCNPSL DIKELIHELNKTNGLFKLVRIMRRRFQEAAAEG+GA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240

Query: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG 300
           PALSLHQGSS+ISTSAP LSSSTE S+SSTEEIN+DEVEETNKHSKNILPRK PAIHSSG
Sbjct: 241 PALSLHQGSSVISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG 300

Query: 301 SAFSLRRSPRFKVRK 316
           SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315

BLAST of Cmc02g0058361 vs. ExPASy TrEMBL
Match: A0A6J1BYG5 (Kinetochore protein SPC25 OS=Momordica charantia OX=3673 GN=LOC111006883 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.1e-129
Identity = 257/316 (81.33%), Postives = 281/316 (88.92%), Query Frame = 0

Query: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60
           ME K  DS+R ++E LR+VRE EIPVQQQKMESFADSF KS+ESI+VRSR NALSQGKLG
Sbjct: 1   MENKVEDSIRVKMESLRMVREKEIPVQQQKMESFADSFRKSLESITVRSRENALSQGKLG 60

Query: 61  ELKARLRVAEDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
           ELKARLR AEDELVK LAVKTRKEAKRL I DS+A SKSRIEELRNTLQDCKA+RDEY+T
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLAITDSLAASKSRIEELRNTLQDCKAKRDEYST 120

Query: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSL LSSSE KETQES+CR+EIQEAISWY RVLDFHIEGG GVKFSFKKINI+ PD
Sbjct: 121 IISQQSLALSSSEHKETQESDCRSEIQEAISWYNRVLDFHIEGGRGVKFSFKKININIPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240
           +EYSFTIRHA+DTY LLDCNPSL DI++LIHELNKTNGLFK VR+MR+RFQE AAEG GA
Sbjct: 181 KEYSFTIRHASDTYTLLDCNPSLQDIQDLIHELNKTNGLFKFVRVMRQRFQEIAAEGVGA 240

Query: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEI-NMDEVEETNKHSKNILPRKKPAIHSS 300
            ALSLHQ S+IIS SAPGLSSST+RS+SST+EI N   +EETNKHSK IL  KKPA+ S 
Sbjct: 241 QALSLHQDSTIISVSAPGLSSSTDRSESSTQEIDNQVRLEETNKHSKKILSGKKPAVLSP 300

Query: 301 GSAFSLRRSPRFKVRK 316
           GSAFSLRRSPRFKVRK
Sbjct: 301 GSAFSLRRSPRFKVRK 316

BLAST of Cmc02g0058361 vs. TAIR 10
Match: AT3G48210.1 (CONTAINS InterPro DOMAIN/s: Kinetochore-Ndc80 complex, subunit Spc25 (InterPro:IPR013255); Has 194 Blast hits to 194 proteins in 72 species: Archae - 0; Bacteria - 4; Metazoa - 72; Fungi - 39; Plants - 62; Viruses - 0; Other Eukaryotes - 17 (source: NCBI BLink). )

HSP 1 Score: 198.4 bits (503), Expect = 8.8e-51
Identity = 131/314 (41.72%), Postives = 191/314 (60.83%), Query Frame = 0

Query: 3   IKATDSVRARLEKLRIVRENEIPVQQQKMESF-ADSFCKSVESISVRSRANALSQGKLGE 62
           I   D+ +  +  L ++ E +I  Q+ K++SF A  F +S+ S+  R++A A SQ +L  
Sbjct: 7   IAGGDTTKETMASLGLICEKDIHEQRLKIDSFIASPFRRSMNSLVERAQATAQSQVELMN 66

Query: 63  LKARLRVAEDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATI 122
           LKA LR AEDELVK LAVKTRKEA+++ I DSI+ ++SRIE LR  LQ  K+++D+   I
Sbjct: 67  LKADLREAEDELVKVLAVKTRKEARQMGIRDSISATQSRIEVLRRNLQLQKSKKDDSVRI 126

Query: 123 ISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDE 182
           ISQQ   LS S+    + +E + +I EAISWY   L FH+E G+GVKF+F  I+   P  
Sbjct: 127 ISQQLQALSKSKDNAGKVTEDKADIHEAISWYNHALGFHVEAGHGVKFTFTNIDAKRPTR 186

Query: 183 EYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAP 242
           E+SFT+ + ND Y LLD +  L  I E++ ELNKTN LF+ VR+MR +F ++        
Sbjct: 187 EFSFTVHYGNDIYTLLDSDLQLDYINEMVQELNKTNDLFRFVRLMREQFLKSTLSELPTH 246

Query: 243 ALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGS 302
           +  L Q +S IS SAP +S ST+ ++ ST E    +V+   +  +      +  + +  S
Sbjct: 247 SGQLQQETSAISASAPAISFSTD-TNMSTPENKRSKVQVNRRQKRG----SESPLLAPVS 306

Query: 303 AFSLRRSPRFKVRK 316
             + RRS RFK +K
Sbjct: 307 TSATRRSSRFKGKK 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008464538.14.7e-16399.68PREDICTED: probable kinetochore protein SPC25 [Cucumis melo][more]
KAA0057836.12.1e-15585.99putative kinetochore protein SPC25 [Cucumis melo var. makuwa][more]
TYJ98520.18.0e-15585.71putative kinetochore protein SPC25 [Cucumis melo var. makuwa][more]
XP_004138099.11.5e-15394.92kinetochore protein SPC25 homolog [Cucumis sativus] >KGN63556.1 hypothetical pro... [more]
XP_038880541.15.6e-14088.61kinetochore protein SPC25 homolog [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q93VK91.2e-4941.72Kinetochore protein SPC25 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC25 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A1S3CM712.3e-16399.68Kinetochore protein SPC25 OS=Cucumis melo OX=3656 GN=LOC103502389 PE=3 SV=1[more]
A0A5A7UUK01.0e-15585.99Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A5D3BFE93.9e-15585.71Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A0A0LU847.3e-15494.92Kinetochore protein SPC25 OS=Cucumis sativus OX=3659 GN=Csa_1G004230 PE=3 SV=1[more]
A0A6J1BYG52.1e-12981.33Kinetochore protein SPC25 OS=Momordica charantia OX=3673 GN=LOC111006883 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT3G48210.18.8e-5141.72CONTAINS InterPro DOMAIN/s: Kinetochore-Ndc80 complex, subunit Spc25 (InterPro:I... [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 94..121
NoneNo IPR availableGENE3D3.30.457.50Chromosome segregation protein Spc25coord: 138..237
e-value: 5.4E-25
score: 89.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 269..287
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 253..268
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 253..315
NoneNo IPR availablePANTHERPTHR14281:SF3SUBFAMILY NOT NAMEDcoord: 4..313
IPR013255Chromosome segregation protein Spc25, C-terminalPFAMPF08234Spindle_Spc25coord: 166..231
e-value: 2.4E-20
score: 72.5
IPR045143Kinetochore protein Spc25PANTHERPTHR14281KINETOCHORE PROTEIN SPC25-RELATEDcoord: 4..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc02g0058361.1Cmc02g0058361.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007049 cell cycle
biological_process GO:0051301 cell division
biological_process GO:0007059 chromosome segregation
cellular_component GO:0031262 Ndc80 complex
cellular_component GO:0005634 nucleus