Cmc02g0052971 (gene) Melon (Charmono) v1.1

BLAST of Cmc02g0052971 vs. NCBI nr
Match: XP_008453615.1 (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo] >TYK11846.1 4-coumarate--CoA ligase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1077.0 bits (2784), Expect = 0.0e+00
Identity = 551/552 (99.82%), Postives = 552/552 (100.00%), Query Frame = 0

Query: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60
           MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120
           ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180
           AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240
           LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300
           CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360
           SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420
           EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480
           DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540
           NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 541 QLRALLAASILN 553
           QLRALLAASIL+
Sbjct: 541 QLRALLAASILS 552

BLAST of Cmc02g0052971 vs. NCBI nr
Match: KAA0058290.1 (4-coumarate--CoA ligase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 551/552 (99.82%), Postives = 551/552 (99.82%), Query Frame = 0

Query: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60
           MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120
           A SRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ
Sbjct: 61  AISRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180
           AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240
           LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300
           CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360
           SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420
           EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480
           DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540
           NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 541 QLRALLAASILN 553
           QLRALLAASILN
Sbjct: 541 QLRALLAASILN 552

BLAST of Cmc02g0052971 vs. NCBI nr
Match: XP_004146311.1 (4-coumarate--CoA ligase 1 [Cucumis sativus] >KGN65444.1 hypothetical protein Csa_019996 [Cucumis sativus])

HSP 1 Score: 988.0 bits (2553), Expect = 3.2e-284
Identity = 508/550 (92.36%), Postives = 528/550 (96.00%), Query Frame = 0

Query: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60
           MAAAD SSPEF+FRSKLPEIPISTHLPLHTYCFENISEFK RPCLINAATGH YTYGEVQ
Sbjct: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120
           ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFL ASYLGAAATMANPFFM +EIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120

Query: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180
           AVSSGAKVIITQ+AFAEKVK LSQ +EMIIKVIFIDD+DPP     +FSSLI+DVAKEEE
Sbjct: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPP-----QFSSLIEDVAKEEE 180

Query: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240
           LEMGDVKISP+DVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHL+IRSDDVVL
Sbjct: 181 LEMGDVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVL 240

Query: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300
           CLLPLFHIYSLNSIMMC+LRVGAAIL+VQKFD+NSL+ELV KYKVTFAP VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAK 300

Query: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360
           SPAVDHFDMSSLR+VLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAF K
Sbjct: 301 SPAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVK 360

Query: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420
           EGFGIKSGGCGTIMRNSEMKI+  QTG SLPRN+TGEICIRS QMMKGYLNDE+ATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAII 420

Query: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480
           D+DGWLHTGDIG+VDDDDELFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM
Sbjct: 421 DEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPM 480

Query: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540
           NDEVAGEVPV FIVRFDGSNITEDEIKQ+ISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 541 QLRALLAASI 551
           QLRALLAASI
Sbjct: 541 QLRALLAASI 545

BLAST of Cmc02g0052971 vs. NCBI nr
Match: XP_038878634.1 (4-coumarate--CoA ligase 1-like [Benincasa hispida])

HSP 1 Score: 929.5 bits (2401), Expect = 1.4e-266
Identity = 473/555 (85.23%), Postives = 501/555 (90.27%), Query Frame = 0

Query: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60
           MAA + SSPEF+FRSKLPEI I THLPLHTY FE +SEF  RPCLINA TG T+TYGEV 
Sbjct: 1   MAATEDSSPEFIFRSKLPEISIPTHLPLHTYLFEKVSEFTNRPCLINATTGETFTYGEVH 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120
            TSRRVAAGLHKLGI KGDVIMLLLQNTP+FV AFL ASYLGAAAT ANPFF PAEI KQ
Sbjct: 61  ETSRRVAAGLHKLGIRKGDVIMLLLQNTPQFVFAFLGASYLGAAATTANPFFTPAEITKQ 120

Query: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFI---DDDDPPTKGGYKFSSLIDDVAK 180
           A SS  K+IITQ+AFAEKVKKLSQ ++ IIKVIFI   DDDDPP  GGY FSSL DDVAK
Sbjct: 121 AASSSIKLIITQAAFAEKVKKLSQENKAIIKVIFIDDDDDDDPPPDGGYHFSSLTDDVAK 180

Query: 181 EEELEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDD 240
           EEE+EMGD K+SPDDVVALPYSSGTTGLPKGVMLTHKG V  VAQQVDGENPH  I+SDD
Sbjct: 181 EEEMEMGDAKVSPDDVVALPYSSGTTGLPKGVMLTHKGLVAIVAQQVDGENPHFNIQSDD 240

Query: 241 VVLCLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLA 300
           VVLC+LPLFHIYSLNSIMMCSLRVGAAILIVQKFD+N LL L+PKYKVT AP+VPPIVLA
Sbjct: 241 VVLCVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNLLLGLIPKYKVTIAPIVPPIVLA 300

Query: 301 IAKSPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLA 360
           I KSPAVDH DMSSLR+V+SGAAPLGKNLEDAFR KLPHVILGQGYGMTE+GS MTMSLA
Sbjct: 301 IVKSPAVDHSDMSSLRIVMSGAAPLGKNLEDAFRTKLPHVILGQGYGMTESGSAMTMSLA 360

Query: 361 FAKEGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATK 420
           FAKEGF IKSGGCGTIMRN+EMKI+ PQTG SLPRN+ GEICI+S Q+MKGYLNDEEATK
Sbjct: 361 FAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQIMKGYLNDEEATK 420

Query: 421 AIIDKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAV 480
            IIDKDGWLHTGD+G+VDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAV
Sbjct: 421 GIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAV 480

Query: 481 IPMNDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKI 540
           IPM DEVAGEVPV FI R DG+NITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKI
Sbjct: 481 IPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKI 540

Query: 541 LRRQLRALLAASILN 553
           LRRQLRALLAASI N
Sbjct: 541 LRRQLRALLAASIPN 555

BLAST of Cmc02g0052971 vs. NCBI nr
Match: KAG6587759.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 856.3 bits (2211), Expect = 1.5e-244
Identity = 450/552 (81.52%), Postives = 485/552 (87.86%), Query Frame = 0

Query: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60
           MAAAD  SPEF+FRSKLP+I I  HLPLHTYCFENIS+FK RPCLIN ATG TYTY EV 
Sbjct: 1   MAAAD-PSPEFIFRSKLPDITIPDHLPLHTYCFENISQFKHRPCLINGATGDTYTYDEVH 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120
            T+RRVAAGLHKLG+GKGDVIMLLLQN+P+FVLAFL ASY+GA ATMANPFF P EIAK 
Sbjct: 61  VTARRVAAGLHKLGVGKGDVIMLLLQNSPQFVLAFLGASYIGAVATMANPFFTPPEIAKH 120

Query: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180
           A SSGAK+IITQ+AFAEKVK L   + + IK+IFI   DPP  G   FS L DDV +E  
Sbjct: 121 AASSGAKLIITQAAFAEKVKSLVDKNGVSIKLIFI---DPPPYGEVHFSVLTDDVKEE-- 180

Query: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240
            EM DVKISP+DVVALPYSSGTTGLPKGVMLTH+G VTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 -EMPDVKISPNDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300
           C+LPLFHIYSLNSIMMCSLRVGAAILIVQKFDIN ++ELV KYKVT AP+VPPIVLAIAK
Sbjct: 241 CVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINYVVELVGKYKVTIAPLVPPIVLAIAK 300

Query: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360
           SPAV   +MSS+RMVLSGAAPLGK LEDAFRAKLP  ILGQGYGMTEAGS +T+SLAFAK
Sbjct: 301 SPAVGGTEMSSIRMVLSGAAPLGKELEDAFRAKLPDAILGQGYGMTEAGSALTLSLAFAK 360

Query: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420
           E F IKSG CGT+MRNS+MKIV PQTG SLPRN+ GEI IRS  +MKGYLN+EEATKAII
Sbjct: 361 ERFPIKSGACGTLMRNSDMKIVNPQTGASLPRNQPGEIWIRSPHVMKGYLNNEEATKAII 420

Query: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480
           D+ GWLHTGDIG+VD+DDE+FIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM
Sbjct: 421 DEHGWLHTGDIGFVDEDDEVFIVDRLKELIKYKGFQVAPAELEALLISHTHIADAAVIPM 480

Query: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540
            +EVAGEVPV FIVR  GSNITEDEIKQYISKQVVFYKRI+RVFFVDSIPKSPSGKILRR
Sbjct: 481 KNEVAGEVPVAFIVRSGGSNITEDEIKQYISKQVVFYKRIHRVFFVDSIPKSPSGKILRR 540

Query: 541 QLRALLAASILN 553
           QLR LLAA I N
Sbjct: 541 QLRELLAARIPN 545

BLAST of Cmc02g0052971 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 9.0e-213
Identity = 379/545 (69.54%), Postives = 443/545 (81.28%), Query Frame = 0

Query: 8   SPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQATSRRVA 67
           S + +FRSKLP+I I  HLPLH+YCFEN+SEF  RPCLI+ A    YTY EV+ TSR+VA
Sbjct: 10  SGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVA 69

Query: 68  AGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQAVSSGAK 127
            GL+KLGI + D IM+LL N PEFV AF+ ASYLGA +TMANP F PAE+ KQA +S AK
Sbjct: 70  VGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAK 129

Query: 128 VIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEELEMGDVK 187
           ++ITQ+ FA KVK      E  +KVI +D      +G   FS LI    + +E E+ DVK
Sbjct: 130 IVITQACFAGKVK--DYAIENDLKVICVDS---VPEGCVHFSELI----QSDEHEIPDVK 189

Query: 188 ISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVLCLLPLFH 247
           I PDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGEN +LY+ SDDV++C+LPLFH
Sbjct: 190 IQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFH 249

Query: 248 IYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAKSPAVDHF 307
           IYSLNS+++C+LRVGAAILI+QKFDI   LEL+PK+KVT  P VPPIVLAIAKSP VD++
Sbjct: 250 IYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNY 309

Query: 308 DMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAKEGFGIKS 367
           D+SS+R V+SGAAPLGK LEDA RAK P+  LGQGYGMTEAG V+ M LAFAKE F IKS
Sbjct: 310 DLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKS 369

Query: 368 GGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAIIDKDGWLH 427
           G CGT++RN+EMKIV P TG SLPRN+ GEICIR  Q+MKGYLND EAT   I+K+GWLH
Sbjct: 370 GACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLH 429

Query: 428 TGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMNDEVAGE 487
           TGDIG++DDDDELFIVDRLKELIKYKGFQVAPAELEALLI+H  I+DAAV+PM DE AGE
Sbjct: 430 TGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGE 489

Query: 488 VPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLA 547
           VPV F+VR +GS ITEDE+K +ISKQV+FYKRI RVFFV+++PKSPSGKILR+ LRA LA
Sbjct: 490 VPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLA 545

Query: 548 ASILN 553
           A I N
Sbjct: 550 AGISN 545

BLAST of Cmc02g0052971 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 4.5e-212
Identity = 378/545 (69.36%), Postives = 442/545 (81.10%), Query Frame = 0

Query: 8   SPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQATSRRVA 67
           S + +FRSKLP+I I  HLPLH+YCFEN+SEF  RPCLI+ A    YTY EV+ TSR+VA
Sbjct: 10  SGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVA 69

Query: 68  AGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQAVSSGAK 127
            GL+KLGI + D IM+LL N PEFV AF+ ASYLGA +TMANP F PAE+ KQA +S AK
Sbjct: 70  VGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAK 129

Query: 128 VIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEELEMGDVK 187
           ++ITQ+ FA KVK      E  +KVI +D      +G   FS LI    + +E E+ DVK
Sbjct: 130 IVITQACFAGKVK--DYAIENDLKVICVDS---APEGCVHFSELI----QSDEHEIPDVK 189

Query: 188 ISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVLCLLPLFH 247
           I PDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGEN +LY+ SDDV++C+LPLFH
Sbjct: 190 IQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFH 249

Query: 248 IYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAKSPAVDHF 307
           IYSLNS+++C+LRVGAAILI+QKFDI   LEL+PK+KVT  P VPPIVLAIAKSP V ++
Sbjct: 250 IYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNY 309

Query: 308 DMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAKEGFGIKS 367
           D+SS+R V+SGAAPLGK LEDA RAK P+  LGQGYGMTEAG V+ M LAFAKE F IKS
Sbjct: 310 DLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKS 369

Query: 368 GGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAIIDKDGWLH 427
           G CGT++RN+EMKIV P TG SLPRN+ GEICIR  Q+MKGYLND EAT   I+K+GWLH
Sbjct: 370 GACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLH 429

Query: 428 TGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMNDEVAGE 487
           TGDIG++DDDDELFIVDRLKELIKYKGFQVAPAELEALLI+H  I+DAAV+PM DE AGE
Sbjct: 430 TGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGE 489

Query: 488 VPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLA 547
           VPV F+VR +GS ITEDE+K +ISKQV+FYKRI RVFFV+++PKSPSGKILR+ LRA LA
Sbjct: 490 VPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLA 545

Query: 548 ASILN 553
           A I N
Sbjct: 550 AGISN 545

BLAST of Cmc02g0052971 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 7.6e-212
Identity = 371/542 (68.45%), Postives = 443/542 (81.73%), Query Frame = 0

Query: 5   DGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQATSR 64
           D    EF+FRSKLP+I I  HLPLH+YCFENIS+FK RPCLIN ATG   TY +V  TSR
Sbjct: 7   DDHQEEFIFRSKLPDIYIPNHLPLHSYCFENISQFKDRPCLINGATGEIITYADVDLTSR 66

Query: 65  RVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQAVSS 124
           +VAAGL KLGI +GDVIMLLLQN+PEFV AFLAASY+GA  T ANPF+ PAE+AKQA +S
Sbjct: 67  KVAAGLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQAAAS 126

Query: 125 GAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEELEMG 184
             K++IT + + +KVK+ + G E  +KV+ + D  PP      FS L     + +E E+ 
Sbjct: 127 KTKLVITLAGYIDKVKEFT-GGESGVKVMCV-DAPPPESECLHFSEL----TQADETEIP 186

Query: 185 DVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVLCLLP 244
            VKI PDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDG+NP+LY   +DV+LC+LP
Sbjct: 187 AVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLP 246

Query: 245 LFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAKSPAV 304
           LFHIYSLNSI++C LRVGAAILI+QKF+I+ LLEL+ K+KVT AP VPPIVL++AK P +
Sbjct: 247 LFHIYSLNSILLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDL 306

Query: 305 DHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAKEGFG 364
             +D+SS+R V+SG AP+GK LEDA + KLPH  LGQGYGMTEAG V++M LAFAKE F 
Sbjct: 307 HRYDLSSIRTVMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFP 366

Query: 365 IKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAIIDKDG 424
           IKSG CGT++RN+EMKIV P TG SLPRN++GEICIR  Q+MKGY+ND EATK  ID+ G
Sbjct: 367 IKSGACGTVVRNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGG 426

Query: 425 WLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMNDEV 484
           WLHTGDIG++D+DDELFIVDRLKELIKYKGFQVAPAELE++LISH +I DAAV+PM DE 
Sbjct: 427 WLHTGDIGFIDNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEA 486

Query: 485 AGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRA 544
           AGEVPV F+VR +GS ITE++IKQYISKQVVFYKRIN+ FF++ IPK+PSGKILR+ LRA
Sbjct: 487 AGEVPVAFVVRSNGSKITEEDIKQYISKQVVFYKRINKAFFIEEIPKNPSGKILRKILRA 542

Query: 545 LL 547
            L
Sbjct: 547 KL 542

BLAST of Cmc02g0052971 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 6.5e-211
Identity = 376/548 (68.61%), Postives = 439/548 (80.11%), Query Frame = 0

Query: 5   DGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQATSR 64
           D    + +FRSKLP+I I  HLPLH+YCFENISEF  RPCLIN A    YTY +V+  SR
Sbjct: 4   DTKQVDIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSR 63

Query: 65  RVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQAVSS 124
           +VAAGLHK GI   D IM+LL N+PEFV AF+ ASYLGA +TMANP F PAE+ KQA +S
Sbjct: 64  KVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKAS 123

Query: 125 GAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEELEMG 184
            AK+I+TQ+    KVK      E  +K+I ID      +G   FS L     +  E ++ 
Sbjct: 124 SAKIIVTQACHVNKVK--DYAFENDVKIICIDS---APEGCLHFSVL----TQANEHDIP 183

Query: 185 DVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVLCLLP 244
           +V+I PDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENP+LYI S+DV+LC+LP
Sbjct: 184 EVEIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLP 243

Query: 245 LFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAKSPAV 304
           LFHIYSLNS+++C LRVGAAILI+QKFDI S LEL+ +YKVT  P VPPIVLAIAKSP V
Sbjct: 244 LFHIYSLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMV 303

Query: 305 DHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAKEGFG 364
           D +D+SS+R V+SGAAPLGK LED  RAK P+  LGQGYGMTEAG V+ M LAFAKE F 
Sbjct: 304 DDYDLSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFE 363

Query: 365 IKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAIIDKDG 424
           IKSG CGT++RN+EMKIV P+TG SLPRN++GEICIR  Q+MKGYLND EAT   IDK+G
Sbjct: 364 IKSGACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEG 423

Query: 425 WLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMNDEV 484
           WL+TGDIGY+DDDDELFIVDRLKELIKYKGFQVAPAELEALL++H +I+DAAV+PM DE 
Sbjct: 424 WLYTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQ 483

Query: 485 AGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRA 544
           AGEVPV F+VR +GS ITEDE+K +ISKQV+FYKRI RVFFVD+IPKSPSGKILR+ LRA
Sbjct: 484 AGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRA 542

Query: 545 LLAASILN 553
            LAA + N
Sbjct: 544 KLAAGLPN 542

BLAST of Cmc02g0052971 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 8.4e-211
Identity = 375/545 (68.81%), Postives = 439/545 (80.55%), Query Frame = 0

Query: 8   SPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQATSRRVA 67
           S + +FRSKLP+I I  HLPLH+YCFENISEF  RPCLIN A    YTY EV+ T R+VA
Sbjct: 12  SGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVA 71

Query: 68  AGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQAVSSGAK 127
            GL+KLGI + D IM+LL N+PEFV AF+ ASYLGA +TMANP F PAE+ KQA +S AK
Sbjct: 72  VGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAK 131

Query: 128 VIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEELEMGDVK 187
           +IITQS F  KVK     SE  +KVI ID      +G   FS L     + +E E+ +VK
Sbjct: 132 IIITQSCFVGKVK--DYASENDVKVICIDS---APEGCLHFSEL----TQSDEHEIPEVK 191

Query: 188 ISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVLCLLPLFH 247
           I PDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGEN +LY+ S+DV++C+LPLFH
Sbjct: 192 IQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFH 251

Query: 248 IYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAKSPAVDHF 307
           IYSLNSI++C LRVGAAILI+QKFDI   LEL+ KYKV+  P VPPIVLAIAKSP VD +
Sbjct: 252 IYSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSY 311

Query: 308 DMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAKEGFGIKS 367
           D+SS+R V+SGAAPLGK LEDA R K P+  LGQGYGMTEAG V+ M LAFAKE F IKS
Sbjct: 312 DLSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKS 371

Query: 368 GGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAIIDKDGWLH 427
           G CGT++RN+EMKIV P TG SLPRN+ GEICIR  Q+MKGYLND EAT   IDK+GWLH
Sbjct: 372 GACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLH 431

Query: 428 TGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMNDEVAGE 487
           TGDIG++D+DDELFIVDRLKELIKYKGFQVAPAE+EALL++H +I+DAAV+PM DE AGE
Sbjct: 432 TGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGE 491

Query: 488 VPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLA 547
           VPV F+VR +GS ITEDE+K +ISKQV+FYKR+ RVFFV+++PKSPSGKILR+ LRA LA
Sbjct: 492 VPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLA 547

Query: 548 ASILN 553
           A + N
Sbjct: 552 AGVPN 547

BLAST of Cmc02g0052971 vs. ExPASy TrEMBL
Match: A0A5D3CN41 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00670 PE=4 SV=1)

HSP 1 Score: 1077.0 bits (2784), Expect = 0.0e+00
Identity = 551/552 (99.82%), Postives = 552/552 (100.00%), Query Frame = 0

Query: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60
           MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120
           ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180
           AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240
           LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300
           CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360
           SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420
           EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480
           DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540
           NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 541 QLRALLAASILN 553
           QLRALLAASIL+
Sbjct: 541 QLRALLAASILS 552

BLAST of Cmc02g0052971 vs. ExPASy TrEMBL
Match: A0A1S3BWP0 (4-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103494274 PE=4 SV=1)

HSP 1 Score: 1077.0 bits (2784), Expect = 0.0e+00
Identity = 551/552 (99.82%), Postives = 552/552 (100.00%), Query Frame = 0

Query: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60
           MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120
           ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180
           AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240
           LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300
           CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360
           SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420
           EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480
           DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540
           NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 541 QLRALLAASILN 553
           QLRALLAASIL+
Sbjct: 541 QLRALLAASILS 552

BLAST of Cmc02g0052971 vs. ExPASy TrEMBL
Match: A0A5A7UT30 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G006230 PE=4 SV=1)

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 551/552 (99.82%), Postives = 551/552 (99.82%), Query Frame = 0

Query: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60
           MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120
           A SRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ
Sbjct: 61  AISRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180
           AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240
           LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300
           CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360
           SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420
           EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480
           DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540
           NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 541 QLRALLAASILN 553
           QLRALLAASILN
Sbjct: 541 QLRALLAASILN 552

BLAST of Cmc02g0052971 vs. ExPASy TrEMBL
Match: A0A0A0LWT6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423020 PE=4 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 1.6e-284
Identity = 508/550 (92.36%), Postives = 528/550 (96.00%), Query Frame = 0

Query: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60
           MAAAD SSPEF+FRSKLPEIPISTHLPLHTYCFENISEFK RPCLINAATGH YTYGEVQ
Sbjct: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120
           ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFL ASYLGAAATMANPFFM +EIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120

Query: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180
           AVSSGAKVIITQ+AFAEKVK LSQ +EMIIKVIFIDD+DPP     +FSSLI+DVAKEEE
Sbjct: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPP-----QFSSLIEDVAKEEE 180

Query: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240
           LEMGDVKISP+DVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHL+IRSDDVVL
Sbjct: 181 LEMGDVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVL 240

Query: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300
           CLLPLFHIYSLNSIMMC+LRVGAAIL+VQKFD+NSL+ELV KYKVTFAP VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAK 300

Query: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360
           SPAVDHFDMSSLR+VLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAF K
Sbjct: 301 SPAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVK 360

Query: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420
           EGFGIKSGGCGTIMRNSEMKI+  QTG SLPRN+TGEICIRS QMMKGYLNDE+ATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAII 420

Query: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480
           D+DGWLHTGDIG+VDDDDELFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM
Sbjct: 421 DEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPM 480

Query: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540
           NDEVAGEVPV FIVRFDGSNITEDEIKQ+ISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 541 QLRALLAASI 551
           QLRALLAASI
Sbjct: 541 QLRALLAASI 545

BLAST of Cmc02g0052971 vs. ExPASy TrEMBL
Match: A0A6J1KTJ2 (4-coumarate--CoA ligase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497098 PE=4 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 6.0e-244
Identity = 450/552 (81.52%), Postives = 482/552 (87.32%), Query Frame = 0

Query: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60
           MAAAD  SPEF+FRSKLP+I I  HLPLHTYCFENIS+FK RPCLIN ATG TYTY EV 
Sbjct: 1   MAAAD-LSPEFIFRSKLPDITIPDHLPLHTYCFENISQFKHRPCLINGATGDTYTYDEVH 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120
            TSRRVAAGLHKLGIGKGDVIMLLLQN+P FVLAFL ASY+GA  TMANPFF   EIAK 
Sbjct: 61  VTSRRVAAGLHKLGIGKGDVIMLLLQNSPHFVLAFLGASYIGAVVTMANPFFTLPEIAKH 120

Query: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180
             SSGAK+IITQ+AFAEKVK L   + + IK+IFI   DPP  G   FS L DDV +E  
Sbjct: 121 VASSGAKLIITQAAFAEKVKSLVDKNGVSIKLIFI---DPPPDGEAHFSVLTDDVKEE-- 180

Query: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240
            +M DVKISP+DVVALPYSSGTTGLPKGVMLTH+G VTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 -DMPDVKISPNDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300
           C+LPLFHIYSLNSIMMCSLRVGAAILIVQKFDIN ++ELV KYKVT AP+VPPIVLAIAK
Sbjct: 241 CVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINYVVELVGKYKVTIAPLVPPIVLAIAK 300

Query: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360
           SPAV  ++MSS+RMVLSGAAPLGK LEDAFRAKLP  ILGQGYGMTEAGS +T+SLAFAK
Sbjct: 301 SPAVGGWEMSSIRMVLSGAAPLGKELEDAFRAKLPDAILGQGYGMTEAGSALTLSLAFAK 360

Query: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420
           E F IKSG CGT+MRNSEMKIV PQTG SLPRN+ GEI IRS  +MKGYLN+EEATKAII
Sbjct: 361 ERFQIKSGACGTLMRNSEMKIVSPQTGASLPRNQPGEIWIRSPHVMKGYLNNEEATKAII 420

Query: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480
           D+ GWLHTGDIGYVD+DDE+FIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM
Sbjct: 421 DEHGWLHTGDIGYVDEDDEVFIVDRLKELIKYKGFQVAPAELEALLISHTHIADAAVIPM 480

Query: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540
            +EVAGEVPV FIVR  GSNITEDEIKQYISKQVVFYKRI+RVFFVDSIPKSPSGKILRR
Sbjct: 481 KNEVAGEVPVAFIVRSGGSNITEDEIKQYISKQVVFYKRIHRVFFVDSIPKSPSGKILRR 540

Query: 541 QLRALLAASILN 553
           QLR LLAA I N
Sbjct: 541 QLRELLAARIPN 545

BLAST of Cmc02g0052971 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 706.1 bits (1821), Expect = 2.3e-203
Identity = 358/545 (65.69%), Postives = 429/545 (78.72%), Query Frame = 0

Query: 8   SPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQATSRRVA 67
           S + +FRS+LP+I I  HLPLH Y FENISEF  +PCLIN  TG  YTY +V  TSR++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  AGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQAVSSGAK 127
           AGLH LG+ + DV+M+LL N+PE VL FLAAS++GA  T ANPFF PAEI+KQA +S AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 VIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEELEMGDVK 187
           +I+TQS + +K+K L     +I+      D D   +   +FS L    ++E  ++    K
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTT----DSDAIPENCLRFSELTQ--SEEPRVDSIPEK 197

Query: 188 ISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVLCLLPLFH 247
           ISP+DVVALP+SSGTTGLPKGVMLTHKG VTSVAQQVDGENP+LY   DDV+LC+LP+FH
Sbjct: 198 ISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFH 257

Query: 248 IYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAKSPAVDHF 307
           IY+LNSIM+CSLRVGA ILI+ KF+I  LLE + + KVT A VVPPIVLAIAKSP  + +
Sbjct: 258 IYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKY 317

Query: 308 DMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAKEGFGIKS 367
           D+SS+RMV SGAAPLGK LEDA  AK P+  LGQGYGMTEAG V+ MSL FAKE F +KS
Sbjct: 318 DLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 377

Query: 368 GGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAIIDKDGWLH 427
           G CGT++RN+EMKI+ P TG SLPRN+ GEICIR  Q+MKGYLND  AT + IDKDGWLH
Sbjct: 378 GACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLH 437

Query: 428 TGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMNDEVAGE 487
           TGD+G++DDDDELFIVDRLKELIKYKGFQVAPAELE+LLI H  I D AV+ M +E AGE
Sbjct: 438 TGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGE 497

Query: 488 VPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLA 547
           VPV F+VR   SNI+EDEIKQ++SKQVVFYKRIN+VFF DSIPK+PSGKILR+ LRA LA
Sbjct: 498 VPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLA 556

Query: 548 ASILN 553
             ++N
Sbjct: 558 NGLMN 556

BLAST of Cmc02g0052971 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 705.7 bits (1820), Expect = 3.0e-203
Identity = 354/544 (65.07%), Postives = 429/544 (78.86%), Query Frame = 0

Query: 4   ADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQATS 63
           ++ ++ + +FRSKLP+I I  HL LH Y F+NISEF  +PCLIN  TGH YTY +V   S
Sbjct: 16  SNNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVIS 75

Query: 64  RRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQAVS 123
           R++AA  HKLG+ + DV+MLLL N PEFVL+FLAAS+ GA AT ANPFF PAEIAKQA +
Sbjct: 76  RQIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKA 135

Query: 124 SGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEELEM 183
           S  K+IIT++ + +K+K L     ++I  I  ++  P  +G  +F+ L     +  E+ +
Sbjct: 136 SNTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEV-I 195

Query: 184 GDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVLCLL 243
             V+ISPDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENP+LY  SDDV+LC+L
Sbjct: 196 DSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVL 255

Query: 244 PLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAKSPA 303
           P+FHIY+LNSIM+C LRVGAAILI+ KF+IN LLEL+ + KVT AP+VPPIVLAIAKS  
Sbjct: 256 PMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSE 315

Query: 304 VDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAKEGF 363
            + +D+SS+R+V SGAAPLGK LEDA  AK P+  LGQGYGMTEAG V+ MSL FAKE F
Sbjct: 316 TEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPF 375

Query: 364 GIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAIIDKD 423
            +KSG CGT++RN+EMKIV P TG SL RN+ GEICIR  Q+MKGYLN+  AT   IDKD
Sbjct: 376 PVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKD 435

Query: 424 GWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMNDE 483
           GWLHTGDIG +DDDDELFIVDRLKELIKYKGFQVAPAELEALLI H  I D AV+ M +E
Sbjct: 436 GWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEE 495

Query: 484 VAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLR 543
            AGEVPV F+V+   S ++ED++KQ++SKQVVFYKRIN+VFF +SIPK+PSGKILR+ LR
Sbjct: 496 AAGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLR 555

Query: 544 ALLA 548
           A LA
Sbjct: 556 AKLA 558

BLAST of Cmc02g0052971 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 659.1 bits (1699), Expect = 3.2e-189
Identity = 327/535 (61.12%), Postives = 422/535 (78.88%), Query Frame = 0

Query: 12  LFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQATSRRVAAGLH 71
           +FRSKLP+I I  HLPLHTYCFE +S    +PCLI  +TG +YTYGE     RRVA+GL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  KLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQAVSSGAKVIIT 131
           KLGI KGDVIM+LLQN+ EFV +F+ AS +GA +T ANPF+   E+ KQ  SSGAK+IIT
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 QSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEELEMGDVKISPD 191
            S + +K+K L +       +  I  D+P  +    FS+LI D   E       V I  D
Sbjct: 154 HSQYVDKLKNLGE------NLTLITTDEPTPENCLPFSTLITD--DETNPFQETVDIGGD 213

Query: 192 DVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVLCLLPLFHIYSL 251
           D  ALP+SSGTTGLPKGV+LTHK  +TSVAQQVDG+NP+LY++S+DV+LC+LPLFHIYSL
Sbjct: 214 DAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSL 273

Query: 252 NSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAKSPAVDHFDMSS 311
           NS+++ SLR GA +L++ KF+I +LL+L+ +++VT A +VPP+V+A+AK+P V+ +D+SS
Sbjct: 274 NSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSS 333

Query: 312 LRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAKEGFGIKSGGCG 371
           +R VLSGAAPLGK L+D+ R +LP  ILGQGYGMTEAG V++MSL FAKE    KSG CG
Sbjct: 334 VRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCG 393

Query: 372 TIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAIIDKDGWLHTGDI 431
           T++RN+E+K+V  +T +SL  N+ GEICIR  Q+MK YLND EAT A ID++GWLHTGDI
Sbjct: 394 TVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDI 453

Query: 432 GYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMNDEVAGEVPVT 491
           GYVD+DDE+FIVDRLKE+IK+KGFQV PAELE+LLI+H  IADAAV+P NDEVAGEVPV 
Sbjct: 454 GYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVA 513

Query: 492 FIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALL 547
           F+VR +G++ITE+++K+Y++KQVVFYKR+++VFFV SIPKSPSGKILR+ L+A L
Sbjct: 514 FVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Cmc02g0052971 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 654.1 bits (1686), Expect = 1.0e-187
Identity = 328/511 (64.19%), Postives = 399/511 (78.08%), Query Frame = 0

Query: 4   ADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQATS 63
           ++ ++ + +FRSKLP+I I  HL LH Y F+NISEF  +PCLIN  TGH YTY +V   S
Sbjct: 16  SNNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVIS 75

Query: 64  RRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQAVS 123
           R++AA  HKLG+ + DV+MLLL N PEFVL+FLAAS+ GA AT ANPFF PAEIAKQA +
Sbjct: 76  RQIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKA 135

Query: 124 SGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEELEM 183
           S  K+IIT++ + +K+K L     ++I  I  ++  P  +G  +F+ L     +  E+ +
Sbjct: 136 SNTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEV-I 195

Query: 184 GDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVLCLL 243
             V+ISPDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENP+LY  SDDV+LC+L
Sbjct: 196 DSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVL 255

Query: 244 PLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAKSPA 303
           P+FHIY+LNSIM+C LRVGAAILI+ KF+IN LLEL+ + KVT AP+VPPIVLAIAKS  
Sbjct: 256 PMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSE 315

Query: 304 VDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAKEGF 363
            + +D+SS+R+V SGAAPLGK LEDA  AK P+  LGQGYGMTEAG V+ MSL FAKE F
Sbjct: 316 TEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPF 375

Query: 364 GIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAIIDKD 423
            +KSG CGT++RN+EMKIV P TG SL RN+ GEICIR  Q+MKGYLN+  AT   IDKD
Sbjct: 376 PVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKD 435

Query: 424 GWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMNDE 483
           GWLHTGDIG +DDDDELFIVDRLKELIKYKGFQVAPAELEALLI H  I D AV+ M +E
Sbjct: 436 GWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEE 495

Query: 484 VAGEVPVTFIVRFDGSNITEDEIKQYISKQV 515
            AGEVPV F+V+   S ++ED++KQ++SKQV
Sbjct: 496 AAGEVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of Cmc02g0052971 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 627.9 bits (1618), Expect = 7.9e-180
Identity = 316/543 (58.20%), Postives = 406/543 (74.77%), Query Frame = 0

Query: 8   SPEFLFRSKLPEIPISTHLPLHTYCFENIS----EFKQRPCLINAATGHTYTYGEVQATS 67
           S +F+FRSKLP+I I  HLPL  Y F+  S          C+I+ ATG   TY +VQ   
Sbjct: 24  SHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQTNM 83

Query: 68  RRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQAVS 127
           RR+AAG+H+LGI  GDV+MLLL N+PEF L+FLA +YLGA +T ANPF+   EIAKQA +
Sbjct: 84  RRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAKA 143

Query: 128 SGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEELEM 187
           S AK+IIT+    +K+  L     +I+  +  D D+              ++ + +E E+
Sbjct: 144 SAAKMIITKKCLVDKLTNLKNDGVLIV-CLDDDGDNGVVSSSDDGCVSFTELTQADETEL 203

Query: 188 GDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVLCLL 247
              KISP+D VA+PYSSGTTGLPKGVM+THKG VTS+AQ+VDGENP+L   ++DV+LC L
Sbjct: 204 LKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFL 263

Query: 248 PLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAKSPA 307
           P+FHIY+L+++M+ ++R GAA+LIV +F++N ++EL+ +YKVT  PV PP+VLA  KSP 
Sbjct: 264 PMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKSPE 323

Query: 308 VDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAKEGF 367
            + +D+SS+R++LSGAA L K LEDA R K P+ I GQGYGMTE+G+V   SLAFAK  F
Sbjct: 324 TERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAKNPF 383

Query: 368 GIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAIIDKD 427
             KSG CGT++RN+EMK+V  +TG+SLPRN++GEIC+R  Q+MKGYLND EAT   IDKD
Sbjct: 384 KTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTIDKD 443

Query: 428 GWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMNDE 487
           GWLHTGDIG+VDDDDE+FIVDRLKELIK+KG+QVAPAELEALLISH  I DAAV+ M DE
Sbjct: 444 GWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKDE 503

Query: 488 VAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLR 547
           VA EVPV F+ R  GS +TED++K Y++KQVV YKRI  VFF++ IPK+ SGKILR+ LR
Sbjct: 504 VADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKDLR 563