Cmc02g0050221 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc02g0050221
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	3'-5' exonuclease domain-containing protein
Location	CMiso1.1chr02: 16301757 .. 16302944 (+)
RNA-Seq Expression	Cmc02g0050221
Synteny	Cmc02g0050221

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGTCCGAAAAAAAAGGGGCTATTGCTGAATTAACCAAACTTGCTTCAACGAAAAAGATTGCGTCCAGTGACTTTTCAGAAGTTTGTAGAACTTCAAAGAAGGTATGATATCTTCCCCGTGGCCCTATTTTCTCTCCCAAGGATTGAATTGATTTCTACGGACGGTATGCCCGAATTTACTTGCACTTAGTTGAAGAAAATTTACATACATGATCTCTTGATTAAAATCTGTTTGGTAATATTTTTGTTTTTCGTTTTTGTTTCTGATTTTTTAAAAAACGGATAACATTCTATGTTCATCGTTCTTAAAATATTAGAAATGTTTCTCATTTAGGTTAGAAATTTATGAGAAGAAAAAAAAAAAAAATTCTACCGTTCCGTTTCTTCTTTTTTTTTCCTTATGTTCCTTCTTTTTTCCTCTCGTTCCTTCTTTTTTTCGTTCCTTCTTCTTTCCTCCTTTTTTCGTTCTCTTCGCCCCCTTTCTCTTCTTCAATCTCTTCATCAATCTCTTCATCGCCTTCTTTCCTTTCTAACCTTCAATCTTTTACCTTCGACGATGAAATCCTCACCCTCCTCACTACTTGCCCTTCCATGGTTGACATGTGGATCTCCGAAATCCTCTATATTTACCGTCTTAGACTCCACCATCTCGTCGTTGGTCTTAACATTGAGTGGCGTCCTAGCTCCGATCGTCATTTTCGTAACCTCGTCACCACTTTACAACTCTGTGTCGGTCGCTACTGCCTCATTTTCCAACATCTGCAGATCTGAAATTCCTCAGTCTTTGATCAATTTCTTAGAGGACGATACCTTCACTTTCGTTGGTGTTGGAATCGACAATGACGTTCTGAAGCACTATAGTGATTACGACCTAAATGTTGATAACACCGTTGATCTTAGGGAGTTGGCTGCGGATGAGATATGTGGATGAGATGCAGAGTGATGAGTTGAGGACTGTCGGACTGAAGACTTTGGAGAGAGAGGTTCTTGGGAGGGAGAATGGTAAAATCTTTCTAGGGATTTTGATTTTGGTTGTGAAAATATTTCTAGGGTTTGGTTTTCTTTCCATGGGTTCTATATAGGATAGCAAACGGAATATTATATATGTTAATAACTGTTCTTCCAGTGAATTCATGTTCATTGTATTTTAGTTTTAGCCTTTTTCCTTTAATTTGATTATCAGTTTCT

mRNA sequence

TGGTCCGAAAAAAAAGGGGCTATTGCTGAATTAACCAAACTTGCTTCAACGAAAAAGATTGCGTCCAGTGACTTTTCAGAAGTTTGTAGAACTTCAAAGAAGGTATGATATCTTCCCCGTGGCCCTATTTTCTCTCCCAAGGATTGAATTGATTTCTACGGACGGTATGCCCGAATTTACTTGCACTTAGTTGAAGAAAATTTACATACATGATCTCTTGATTAAAATCTGTTTGGTAATATTTTTGTTTTTCGTTTTTGTTTCTGATTTTTTAAAAAACGGATAACATTCTATGTTCATCGTTCTTAAAATATTAGAAATGTTTCTCATTTAGGTTAGAAATTTATGAGAAGAAAAAAAAAAAAAATTCTACCGTTCCGTTTCTTCTTTTTTTTTCCTTATGTTCCTTCTTTTTTCCTCTCGTTCCTTCTTTTTTTCGTTCCTTCTTCTTTCCTCCTTTTTTCGTTCTCTTCGCCCCCTTTCTCTTCTTCAATCTCTTCATCAATCTCTTCATCGCCTTCTTTCCTTTCTAACCTTCAATCTTTTACCTTCGACGATGAAATCCTCACCCTCCTCACTACTTGCCCTTCCATGGTTGACATGTGGATCTCCGAAATCCTCTATATTTACCGTCTTAGACTCCACCATCTCGTCGTTGGTCTTAACATTGAGTGGCGTCCTAGCTCCGATCGTCATTTTCGTAACCTCGTCACCACTTTACAACTCTGTGTCGGTCGCTACTGCCTCATTTTCCAACATCTGCAGATCTGAAATTCCTCAGTCTTTGATCAATTTCTTAGAGGACGATACCTTCACTTTCGTTGGTGTTGGAATCGACAATGACGTTCTGAAGCACTATAGTGATTACGACCTAAATGTTGATAACACCGTTGATCTTAGGGAGTTGGCTGCGGATGAGATATGTGGATGAGATGCAGAGTGATGAGTTGAGGACTGTCGGACTGAAGACTTTGGAGAGAGAGGTTCTTGGGAGGGAGAATGGTAAAATCTTTCTAGGGATTTTGATTTTGGTTGTGAAAATATTTCTAGGGTTTGGTTTTCTTTCCATGGGTTCTATATAGGATAGCAAACGGAATATTATATATGTTAATAACTGTTCTTCCAGTGAATTCATGTTCATTGTATTTTAGTTTTAGCCTTTTTCCTTTAATTTGATTATCAGTTTCT

Coding sequence (CDS)

ATGTTCCTTCTTTTTTCCTCTCGTTCCTTCTTTTTTTCGTTCCTTCTTCTTTCCTCCTTTTTTCGTTCTCTTCGCCCCCTTTCTCTTCTTCAATCTCTTCATCAATCTCTTCATCGCCTTCTTTCCTTTCTAACCTTCAATCTTTTACCTTCGACGATGAAATCCTCACCCTCCTCACTACTTGCCCTTCCATGGTTGACATGTGGATCTCCGAAATCCTCTATATTTACCGTCTTAGACTCCACCATCTCGTCGTTGGTCTTAACATTGAGTGGCGTCCTAGCTCCGATCGTCATTTTCGTAACCTCGTCACCACTTTACAACTCTGTGTCGGTCGCTACTGCCTCATTTTCCAACATCTGCAGATCTGAAATTCCTCAGTCTTTGATCAATTTCTTAGAGGACGATACCTTCACTTTCGTTGGTGTTGGAATCGACAATGACGTTCTGAAGCACTATAGTGATTACGACCTAAATGTTGATAACACCGTTGATCTTAGGGAGTTGGCTGCGGATGAGATATGTGGATGA

Protein sequence

MFLLFSSRSFFFSFLLLSSFFRSLRPLSLLQSLHQSLHRLLSFLTFNLLPSTMKSSPSSLLALPWLTCGSPKSSIFTVLDSTISSLVLTLSGVLAPIVIFVTSSPLYNSVSVATASFSNICRSEIPQSLINFLEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDLRELAADEICG

Homology

[Close]

BLAST of Cmc02g0050221 vs. NCBI nr
Match: KAE8651170.1 (hypothetical protein Csa_000755 [Cucumis sativus])

HSP 1 Score: 94.7 bits (234), Expect = 8.2e-16
Identity = 48/58 (82.76%), Postives = 51/58 (87.93%), Query Frame = 0


Query: 117 FSNICRSEIPQSLINFLEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDLRELAADEI 175
           F  I  SE PQSLI+FLEDDTFTFVGVGIDNDVLK Y+DYDLNV NTVDLRELAADE+
Sbjct: 91  FQLIHASETPQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEM 148

[Close]

BLAST of Cmc02g0050221 vs. NCBI nr
Match: XP_004136771.1 (Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 94.7 bits (234), Expect = 8.2e-16
Identity = 48/58 (82.76%), Postives = 51/58 (87.93%), Query Frame = 0


Query: 117 FSNICRSEIPQSLINFLEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDLRELAADEI 175
           F  I  SE PQSLI+FLEDDTFTFVGVGIDNDVLK Y+DYDLNV NTVDLRELAADE+
Sbjct: 91  FQLIHASETPQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEM 148

[Close]

BLAST of Cmc02g0050221 vs. NCBI nr
Match: XP_038906336.1 (Werner Syndrome-like exonuclease [Benincasa hispida])

HSP 1 Score: 85.1 bits (209), Expect = 6.5e-13
Identity = 43/58 (74.14%), Postives = 49/58 (84.48%), Query Frame = 0


Query: 117 FSNICRSEIPQSLINFLEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDLRELAADEI 175
           F  I  SEIP+SLI+FLEDDTFTFVGVGID DV + Y+DYDL V NTVDLRELAA+E+
Sbjct: 91  FQLIHTSEIPESLIDFLEDDTFTFVGVGIDQDVQQLYNDYDLKVANTVDLRELAANEM 148

[Close]

BLAST of Cmc02g0050221 vs. NCBI nr
Match: XP_023876352.1 (Werner Syndrome-like exonuclease [Quercus suber])

HSP 1 Score: 72.4 bits (176), Expect = 4.4e-09
Identity = 57/128 (44.53%), Postives = 67/128 (52.34%), Query Frame = 0


Query: 47  NLLPSTMKSSPSSLLALPWLTCGSPKSSIFTVLDSTISSLVLTLSGVLAPIVIFVTSSPL 106
           +LL  T+ S PSS     W T GSP S+  T   ST SS   T SG   P+      + L
Sbjct: 6   SLLSPTI-SRPSSPTLPKWSTPGSPTSTASTAAVSTASSSASTSSG--NPV------ATL 65

Query: 107 YNSVSVATASFSNICRSEIPQSLINFLEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDL 166
              V      F  I  +  P SLI FL D+ FTFVGVGI++DV K   DYDL V N VDL
Sbjct: 66  QLCVGRCCLIFQLIYATHFPNSLIEFLGDEDFTFVGVGIESDVEKLLDDYDLKVGNVVDL 124

Query: 167 RELAADEI 175
           R LA D +
Sbjct: 126 RGLAVDRL 124

[Close]

BLAST of Cmc02g0050221 vs. NCBI nr
Match: XP_023873050.1 (Werner syndrome ATP-dependent helicase homolog [Quercus suber])

HSP 1 Score: 70.1 bits (170), Expect = 2.2e-08
Identity = 56/128 (43.75%), Postives = 67/128 (52.34%), Query Frame = 0


Query: 47  NLLPSTMKSSPSSLLALPWLTCGSPKSSIFTVLDSTISSLVLTLSGVLAPIVIFVTSSPL 106
           +LL  T+ S PSS     W T GSP S+  T   ST SS   T SG   P+      + L
Sbjct: 6   SLLSPTI-SRPSSPTLPKWSTPGSPTSTASTAAVSTTSSSASTSSG--DPV------ATL 65

Query: 107 YNSVSVATASFSNICRSEIPQSLINFLEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDL 166
              V      F  I  +  P SLI FL ++ FTFVGVGI++DV K   DYDL V N VDL
Sbjct: 66  QLCVGRRCLIFQLIYATHFPNSLIEFLGNEDFTFVGVGIESDVEKLLDDYDLKVGNVVDL 124

Query: 167 RELAADEI 175
           R LA D +
Sbjct: 126 RGLAVDRL 124

[Close]

BLAST of Cmc02g0050221 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.8e-05
Identity = 25/58 (43.10%), Postives = 37/58 (63.79%), Query Frame = 0


Query: 119 NICRSEIPQSLINFLEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDLRELAADEICG 177
           +I  S IPQSL + +ED T   VG+GID D +K + DY +++ +  DL +LA  +I G
Sbjct: 165 HIFHSGIPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG 222

[Close]

BLAST of Cmc02g0050221 vs. ExPASy TrEMBL
Match: A0A0A0LHC5 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G819830 PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 4.0e-16
Identity = 48/58 (82.76%), Postives = 51/58 (87.93%), Query Frame = 0


Query: 117 FSNICRSEIPQSLINFLEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDLRELAADEI 175
           F  I  SE PQSLI+FLEDDTFTFVGVGIDNDVLK Y+DYDLNV NTVDLRELAADE+
Sbjct: 91  FQLIHASETPQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEM 148

[Close]

BLAST of Cmc02g0050221 vs. ExPASy TrEMBL
Match: A0A6J1D121 (Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016622 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.1e-08
Identity = 34/56 (60.71%), Postives = 40/56 (71.43%), Query Frame = 0


Query: 117 FSNICRSEIPQSLINFLEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDLRELAAD 173
           F  I   EIP SL++FL D+TFTFVGVGID D  K + DYDL V+N VDLR +A D
Sbjct: 82  FQLIHAPEIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVD 137

[Close]

BLAST of Cmc02g0050221 vs. ExPASy TrEMBL
Match: A0A5D3DF32 (Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold333G00650 PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.3e-08
Identity = 31/42 (73.81%), Postives = 37/42 (88.10%), Query Frame = 0


Query: 133 LEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDLRELAADEI 175
           +EDDTF F+GVGI+ND+LK Y+DYDLNV N VDLRELA DE+
Sbjct: 1   MEDDTFPFIGVGINNDILKLYNDYDLNVANIVDLRELATDEM 42

[Close]

BLAST of Cmc02g0050221 vs. ExPASy TrEMBL
Match: A0A5A7SNJ2 (Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold219G00080 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 3.1e-08
Identity = 30/42 (71.43%), Postives = 37/42 (88.10%), Query Frame = 0


Query: 133 LEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDLRELAADEI 175
           +EDDTF F+GVGI+ND+LK Y+DYDLNV N +DLRELA DE+
Sbjct: 1   MEDDTFPFIGVGINNDILKLYNDYDLNVANIIDLRELATDEM 42

[Close]

BLAST of Cmc02g0050221 vs. ExPASy TrEMBL
Match: A0A5B7BMQ8 (Putative Werner Syndrome-like exonuclease OS=Davidia involucrata OX=16924 GN=Din_038562 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 3.1e-08
Identity = 36/58 (62.07%), Postives = 41/58 (70.69%), Query Frame = 0


Query: 117 FSNICRSEIPQSLINFLEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDLRELAADEI 175
           F  I   EIPQSL +FL D+ +TFVGVGID DV K Y DY L+V NTVDL  LAA E+
Sbjct: 85  FQLIFAPEIPQSLRDFLTDNDYTFVGVGIDKDVQKLYDDYGLDVSNTVDLGYLAASEL 142

[Close]

BLAST of Cmc02g0050221 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 50.8 bits (120), Expect = 1.3e-06
Identity = 25/58 (43.10%), Postives = 37/58 (63.79%), Query Frame = 0


Query: 119 NICRSEIPQSLINFLEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDLRELAADEICG 177
           +I  S IPQSL + +ED T   VG+GID D +K + DY +++ +  DL +LA  +I G
Sbjct: 165 HIFHSGIPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG 222

[Close]

BLAST of Cmc02g0050221 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 50.8 bits (120), Expect = 1.3e-06
Identity = 25/58 (43.10%), Postives = 37/58 (63.79%), Query Frame = 0


Query: 119 NICRSEIPQSLINFLEDDTFTFVGVGIDNDVLKHYSDYDLNVDNTVDLRELAADEICG 177
           +I  S IPQSL + +ED T   VG+GID D +K + DY +++ +  DL +LA  +I G
Sbjct: 165 HIFHSGIPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG 222

The following BLAST results are available for this feature:

BLAST of Cmc02g0050221 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8651170.1	8.2e-16	82.76	hypothetical protein Csa_000755 [Cucumis sativus]	[more]
XP_004136771.1	8.2e-16	82.76	Werner Syndrome-like exonuclease [Cucumis sativus]	[more]
XP_038906336.1	6.5e-13	74.14	Werner Syndrome-like exonuclease [Benincasa hispida]	[more]
XP_023876352.1	4.4e-09	44.53	Werner Syndrome-like exonuclease [Quercus suber]	[more]
XP_023873050.1	2.2e-08	43.75	Werner syndrome ATP-dependent helicase homolog [Quercus suber]	[more]

BLAST of Cmc02g0050221 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84LH3	1.8e-05	43.10	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=...	[more]

BLAST of Cmc02g0050221 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHC5	4.0e-16	82.76	3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A6J1D121	1.1e-08	60.71	Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016622 ...	[more]
A0A5D3DF32	2.3e-08	73.81	Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7SNJ2	3.1e-08	71.43	Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5B7BMQ8	3.1e-08	62.07	Putative Werner Syndrome-like exonuclease OS=Davidia involucrata OX=16924 GN=Din...	[more]

BLAST of Cmc02g0050221 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13870.1	1.3e-06	43.10	Werner syndrome-like exonuclease	[more]
AT4G13870.2	1.3e-06	43.10	Werner syndrome-like exonuclease	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 111..175 e-value: 8.0E-13 score: 50.3
None	No IPR available	PANTHER	PTHR13620:SF65	OS01G0660800 PROTEIN	coord: 122..173
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 122..173
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 122..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc02g0050221.1	Cmc02g0050221.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016020	membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding