Cmc02g0046911 (gene) Melon (Charmono) v1.1

Overview
NameCmc02g0046911
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionReverse transcriptase
LocationCMiso1.1chr02: 12485421 .. 12485720 (-)
RNA-Seq ExpressionCmc02g0046911
SyntenyCmc02g0046911
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGCCTTTGCATTGACTACAGAGAGCTGAAGAAGGCGACAATCAAGAACCGCTACCCCTTGCCCAGGATTGATGACTTGTTTGATCAGTTGCAGGGAGCCACTGTCTTTTCTAAGATCGACCTGCGATCAGGCTACCACCAGTTGAGGATCAGGGATAGTGACATTCCTAAGACGGCCTTTCGTTCGAGATACGGACATTACGAGTTCATTGTGATGTCTTTTGGGTTGACCAATGCTCCTGCGGTATTCATGGACTTGATGAACAGAGTGTTACCATGGAAAATCTTAAGAAACTAA

mRNA sequence

ATGCGCCTTTGCATTGACTACAGAGAGCTGAAGAAGGCGACAATCAAGAACCGCTACCCCTTGCCCAGGATTGATGACTTGTTTGATCAGTTGCAGGGAGCCACTGTCTTTTCTAAGATCGACCTGCGATCAGGCTACCACCAGTTGAGGATCAGGGATAGTGACATTCCTAAGACGGCCTTTCGTTCGAGATACGGACATTACGAGTTCATTGTGATGTCTTTTGGGTTGACCAATGCTCCTGCGGTATTCATGGACTTGATGAACAGAGTGTTACCATGGAAAATCTTAAGAAACTAA

Coding sequence (CDS)

ATGCGCCTTTGCATTGACTACAGAGAGCTGAAGAAGGCGACAATCAAGAACCGCTACCCCTTGCCCAGGATTGATGACTTGTTTGATCAGTTGCAGGGAGCCACTGTCTTTTCTAAGATCGACCTGCGATCAGGCTACCACCAGTTGAGGATCAGGGATAGTGACATTCCTAAGACGGCCTTTCGTTCGAGATACGGACATTACGAGTTCATTGTGATGTCTTTTGGGTTGACCAATGCTCCTGCGGTATTCATGGACTTGATGAACAGAGTGTTACCATGGAAAATCTTAAGAAACTAA

Protein sequence

MRLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTAFRSRYGHYEFIVMSFGLTNAPAVFMDLMNRVLPWKILRN
Homology
BLAST of Cmc02g0046911 vs. NCBI nr
Match: KAA0051386.1 (putative Retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 186.4 bits (472), Expect = 1.2e-43
Identity = 88/91 (96.70%), Postives = 89/91 (97.80%), Query Frame = 0

Query: 1   MRLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 60
           MRLCIDYREL K T+KNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA
Sbjct: 310 MRLCIDYRELNKVTVKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 369

Query: 61  FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 92
           FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV
Sbjct: 370 FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 400

BLAST of Cmc02g0046911 vs. NCBI nr
Match: TYK04344.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 186.4 bits (472), Expect = 1.2e-43
Identity = 88/91 (96.70%), Postives = 89/91 (97.80%), Query Frame = 0

Query: 1   MRLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 60
           MRLCIDYREL K T+KNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA
Sbjct: 414 MRLCIDYRELNKVTVKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 473

Query: 61  FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 92
           FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV
Sbjct: 474 FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 504

BLAST of Cmc02g0046911 vs. NCBI nr
Match: KAA0063946.1 (pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 186.0 bits (471), Expect = 1.5e-43
Identity = 87/91 (95.60%), Postives = 89/91 (97.80%), Query Frame = 0

Query: 1   MRLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 60
           MRLCIDYREL K T+KNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA
Sbjct: 867 MRLCIDYRELNKVTVKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 926

Query: 61  FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 92
           FRSRYGHYEF+VMSFGLTNAPAVFMDLMNRV
Sbjct: 927 FRSRYGHYEFVVMSFGLTNAPAVFMDLMNRV 957

BLAST of Cmc02g0046911 vs. NCBI nr
Match: TYK21327.1 (ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 185.3 bits (469), Expect = 2.6e-43
Identity = 87/91 (95.60%), Postives = 89/91 (97.80%), Query Frame = 0

Query: 1   MRLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 60
           MRLCIDYREL K T+KNRYPLP+IDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA
Sbjct: 254 MRLCIDYRELNKVTVKNRYPLPKIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 313

Query: 61  FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 92
           FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV
Sbjct: 314 FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 344

BLAST of Cmc02g0046911 vs. NCBI nr
Match: KAA0063098.1 (pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 184.9 bits (468), Expect = 3.4e-43
Identity = 87/91 (95.60%), Postives = 88/91 (96.70%), Query Frame = 0

Query: 1   MRLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 60
           MRLCIDYREL K TIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRD DIPKTA
Sbjct: 641 MRLCIDYRELNKVTIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDGDIPKTA 700

Query: 61  FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 92
           FRSRYGHYEF+VMSFGLTNAPAVFMDLMNRV
Sbjct: 701 FRSRYGHYEFVVMSFGLTNAPAVFMDLMNRV 731

BLAST of Cmc02g0046911 vs. ExPASy Swiss-Prot
Match: P31843 (RNA-directed DNA polymerase homolog OS=Oenothera berteroana OX=3950 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 3.2e-28
Identity = 59/92 (64.13%), Postives = 71/92 (77.17%), Query Frame = 0

Query: 1  MRLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 60
          +R+CIDYR L K TIKN+YP+PR+DDLFD+L  AT F+K+DLRSGY Q+RI   D PKT 
Sbjct: 6  LRMCIDYRALTKVTIKNKYPIPRVDDLFDRLAQATWFTKLDLRSGYWQVRIAKGDEPKTT 65

Query: 61 FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRVL 93
            +RYG +EF VM FGLTNA A F +LMN VL
Sbjct: 66 CVTRYGSFEFRVMPFGLTNALATFCNLMNNVL 97

BLAST of Cmc02g0046911 vs. ExPASy Swiss-Prot
Match: Q99315 (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 99.0 bits (245), Expect = 3.2e-20
Identity = 45/87 (51.72%), Postives = 59/87 (67.82%), Query Frame = 0

Query: 2   RLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTAF 61
           RLC+DYR L KATI + +PLPRID+L  ++  A +F+ +DL SGYHQ+ +   D  KTAF
Sbjct: 647 RLCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIFTTLDLHSGYHQIPMEPKDRYKTAF 706

Query: 62  RSRYGHYEFIVMSFGLTNAPAVFMDLM 89
            +  G YE+ VM FGL NAP+ F   M
Sbjct: 707 VTPSGKYEYTVMPFGLVNAPSTFARYM 733

BLAST of Cmc02g0046911 vs. ExPASy Swiss-Prot
Match: Q7LHG5 (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 99.0 bits (245), Expect = 3.2e-20
Identity = 45/87 (51.72%), Postives = 59/87 (67.82%), Query Frame = 0

Query: 2   RLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTAF 61
           RLC+DYR L KATI + +PLPRID+L  ++  A +F+ +DL SGYHQ+ +   D  KTAF
Sbjct: 673 RLCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIFTTLDLHSGYHQIPMEPKDRYKTAF 732

Query: 62  RSRYGHYEFIVMSFGLTNAPAVFMDLM 89
            +  G YE+ VM FGL NAP+ F   M
Sbjct: 733 VTPSGKYEYTVMPFGLVNAPSTFARYM 759

BLAST of Cmc02g0046911 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 7.2e-20
Identity = 42/91 (46.15%), Postives = 61/91 (67.03%), Query Frame = 0

Query: 2   RLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTAF 61
           R+ IDYR+L + TI +RYP+P +D++  +L     F+ IDL  G+HQ+ + +  I KTAF
Sbjct: 262 RVVIDYRKLNEITIPDRYPIPNMDEILGKLGKCQYFTTIDLAKGFHQIEMDEESISKTAF 321

Query: 62  RSRYGHYEFIVMSFGLTNAPAVFMDLMNRVL 93
            ++ GHYE++ M FGL NAPA F   MN +L
Sbjct: 322 STKSGHYEYLRMPFGLRNAPATFQRCMNNIL 352

BLAST of Cmc02g0046911 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 4.7e-19
Identity = 39/91 (42.86%), Postives = 61/91 (67.03%), Query Frame = 0

Query: 2   RLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTAF 61
           R+ IDYR+L + T+ +R+P+P +D++  +L     F+ IDL  G+HQ+ +    + KTAF
Sbjct: 263 RIVIDYRKLNEITVGDRHPIPNMDEILGKLGRCNYFTTIDLAKGFHQIEMDPESVSKTAF 322

Query: 62  RSRYGHYEFIVMSFGLTNAPAVFMDLMNRVL 93
            +++GHYE++ M FGL NAPA F   MN +L
Sbjct: 323 STKHGHYEYLRMPFGLKNAPATFQRCMNDIL 353

BLAST of Cmc02g0046911 vs. ExPASy TrEMBL
Match: A0A5A7UB12 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold55G001260 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.7e-44
Identity = 88/91 (96.70%), Postives = 89/91 (97.80%), Query Frame = 0

Query: 1   MRLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 60
           MRLCIDYREL K T+KNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA
Sbjct: 310 MRLCIDYRELNKVTVKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 369

Query: 61  FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 92
           FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV
Sbjct: 370 FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 400

BLAST of Cmc02g0046911 vs. ExPASy TrEMBL
Match: A0A5D3BZD2 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold675G00070 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.7e-44
Identity = 88/91 (96.70%), Postives = 89/91 (97.80%), Query Frame = 0

Query: 1   MRLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 60
           MRLCIDYREL K T+KNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA
Sbjct: 414 MRLCIDYRELNKVTVKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 473

Query: 61  FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 92
           FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV
Sbjct: 474 FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 504

BLAST of Cmc02g0046911 vs. ExPASy TrEMBL
Match: A0A5A7VBY3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold616G00920 PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 7.4e-44
Identity = 87/91 (95.60%), Postives = 89/91 (97.80%), Query Frame = 0

Query: 1   MRLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 60
           MRLCIDYREL K T+KNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA
Sbjct: 867 MRLCIDYRELNKVTVKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 926

Query: 61  FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 92
           FRSRYGHYEF+VMSFGLTNAPAVFMDLMNRV
Sbjct: 927 FRSRYGHYEFVVMSFGLTNAPAVFMDLMNRV 957

BLAST of Cmc02g0046911 vs. ExPASy TrEMBL
Match: A0A5D3DCG0 (Ty3-gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68479G00060 PE=4 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.3e-43
Identity = 87/91 (95.60%), Postives = 89/91 (97.80%), Query Frame = 0

Query: 1   MRLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 60
           MRLCIDYREL K T+KNRYPLP+IDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA
Sbjct: 254 MRLCIDYRELNKVTVKNRYPLPKIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 313

Query: 61  FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 92
           FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV
Sbjct: 314 FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 344

BLAST of Cmc02g0046911 vs. ExPASy TrEMBL
Match: A0A5A7V646 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1337G00030 PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.6e-43
Identity = 87/91 (95.60%), Postives = 88/91 (96.70%), Query Frame = 0

Query: 1   MRLCIDYRELKKATIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDSDIPKTA 60
           MRLCIDYREL K TIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRD DIPKTA
Sbjct: 641 MRLCIDYRELNKVTIKNRYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIRDGDIPKTA 700

Query: 61  FRSRYGHYEFIVMSFGLTNAPAVFMDLMNRV 92
           FRSRYGHYEF+VMSFGLTNAPAVFMDLMNRV
Sbjct: 701 FRSRYGHYEFVVMSFGLTNAPAVFMDLMNRV 731

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0051386.11.2e-4396.70putative Retrotransposon protein [Cucumis melo var. makuwa][more]
TYK04344.11.2e-4396.70reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0063946.11.5e-4395.60pol protein [Cucumis melo var. makuwa][more]
TYK21327.12.6e-4395.60ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa][more]
KAA0063098.13.4e-4395.60pol protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
P318433.2e-2864.13RNA-directed DNA polymerase homolog OS=Oenothera berteroana OX=3950 PE=4 SV=1[more]
Q993153.2e-2051.72Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20... [more]
Q7LHG53.2e-2051.72Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20... [more]
P208257.2e-2046.15Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste... [more]
P043234.7e-1942.86Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast... [more]
Match NameE-valueIdentityDescription
A0A5A7UB125.7e-4496.70Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold55... [more]
A0A5D3BZD25.7e-4496.70Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold67... [more]
A0A5A7VBY37.4e-4495.60Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold61... [more]
A0A5D3DCG01.3e-4395.60Ty3-gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A5A7V6461.6e-4395.60Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold13... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 3..92
e-value: 8.0E-9
score: 35.3
IPR043128Reverse transcriptase/Diguanylate cyclase domainGENE3D3.30.70.270coord: 19..93
e-value: 9.1E-40
score: 138.2
NoneNo IPR availableGENE3D3.10.10.10HIV Type 1 Reverse Transcriptase, subunit A, domain 1coord: 1..79
e-value: 9.1E-40
score: 138.2
NoneNo IPR availablePANTHERPTHR24559:SF319SUBFAMILY NOT NAMEDcoord: 2..92
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 2..92
NoneNo IPR availableCDDcd01647RT_LTRcoord: 2..92
e-value: 1.53814E-46
score: 145.046
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 1..93

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc02g0046911.1Cmc02g0046911.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0006508 proteolysis
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity
molecular_function GO:0008194 UDP-glycosyltransferase activity
molecular_function GO:0008270 zinc ion binding