Cmc02g0038701 (gene) Melon (Charmono) v1.1

Overview
NameCmc02g0038701
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionGlutathione synthetase
LocationCMiso1.1chr02: 946181 .. 949523 (+)
RNA-Seq ExpressionCmc02g0038701
SyntenyCmc02g0038701
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAAACACTAGCAGAAATCGATAGAGAGGGAGTACTAAGATCTGATGGAACACTCGTTGTGTAAGTAATGTTGAATGTCAAATTTGGTTTTTCCTTCATAAAATTGGTTACAGTTTTATGTTTCTTATAACTCTTGAGAACTTCTCCTCCTGCAGGGATGGCCAAGCAATTGCTGTTGTGTACTTCAGGGCTGGATATACGCCTAGGGACTATCCGTCTGAATCGGTGAGCAACTGCTTCATTCTGCTGCTGCTCGCAGTCGCAGGAGACCTTTTTTGTTTAGGAATTAGGATTAAGTTGATGTATTCTTTTGGGATGGACTTGCTGTTGCTACCATGCATTAGTGTTGCCATTCATTCTAGGAAGAAGCTCACTTTTCTTGATGAATTTCAATAGTTTCATGAGATTTTAAAACAAGGAAAAGTAAATTCAATCCCGGAGGATTTTAGAGTTCCAGACATTGAATTTCTTAGCTACTTTGTCAGACCTTTCTTCATGCTGGCTTTGTGGATGTACCAGAAACCCATTGCTTCTTCTAACTTAAAGTTCTTGTGTGAAAATGTTTGGGAGAAATAAATTATGTAATCTGAAGTGTTGAACCTTCACAATAATTGAAACAATGAGAGTTCATACTCCAGAATCCTCCCCTTTGTCATCTTATGAATTTATCTCCTCAAACTTGAATAACTTATAGATCATTCTAGATGAAGTCTTGGAATTACATTCATCAGAGGAAAAATGTAGACTTTGATTTTCCTTGATTGTGCTCGTTAGATTTTGCAGTTGTCTTTCATCAGCATATCTTATATCTTTCCCACTGTTGTCAAAGCAATGCTGACAGTGACTATTTGCATGTTCGATTGTTGCTAGGTGCTAAGCAATTTGGCATGCTTAAGTTACAAAATGGATTGTATCATAGATGTTATATTACCTGTCCTGACTCATAATAATACTACGCTGAGGTTGCTTGGCATTAAAAAATTCAGAAGACTCTCTTACTCAAGCTCATTGAAGCTTGAGTGTAGGGCCCTCTAAATAGGCATTAAAATTTACCAACATCCACCCATTAAGCCAGATAATGTTGAAGCAATTTTGAACTATTCATATGTACAGACAATTATAACATCTGATGATAATCCTTCCAATTTGATGACCATTATCGATTTCAATACCAAGGAATAAAAGAGTCTCTCTTAAAGTTCTTTAACGTCCTTGTCAATTTATTCAAGTTCATTTTATCTTGAATAAAGCTTCGGAGTTATTTTCTCATTTGAATGGTAACAAATTTCAGGAATGGAGTGCAAGGTTACTTATGGAGCAGTCATCTGCAGTCAAATGCCCATCCATATCTTATCATTTAGTAGGCACCAAGAAAATTCAACAGGAACTTGCAAGACCAAATGTTCTTGAAAGGTATTATTTATTTTTGCATCTTCAGTTCCCATCCTTCCGTTTGACTAGAAAGAAACGTGTTTGGTAAATCATCTGTTTCTTGTTTCTAAATTCTAAAAAAAATTTATAAAACTGAACCAAAATTTATAAAACTAAAAAATGTATAGAAATAAAAGTTTAATTATTGAACTAAAGTGATGTCGCCTATCCTTAAATAAATGTCTCATTATTAAGTCGACACAAGTATATATCAATAGCATGTGGTGTAAAAGATTAATGATCACAAAAAAGTGACAAAGTTTTCAGACAATGTTCAATTCATCTAATTGAACTAACACATGTAAGCCCACATCATCTTAGATGAATTTGTTGGGAATTGATAATTTATATAGGAAATAGTGTTTCTTATTGCAGTTTTTATTTGCAGCTGAAATTTTGAGCCTCTACTCAGGTTCCTTGACAGTGAAGAGGATATTGCCAAGATAAGAAAATGTTTTGCAGGGTTGTGGAGTTTAGATGACAAAGATATAGTTAAAAAAGCCATTGAAACACCGGAACTATTCGTTATGAAGCCACAAAGAGAAGGCGGAGGTCTGTTCCATAACCTTCTGAATTTTTTCAATTTGGTTTACCCAACTTTTCTCTCTCTTTGCAATAAAGCCGTTCACAAAGATGTCATTGATCTTCCTCAGGGAATAATATTTATGGAGATGATGTGAGGGAAGCCCTTCTAAAATTGCAGGAGGAAGGGAGTGAAGAAGATGGTGCTTACATCCTTATGCAGAGGATATTCCCATCTGTCTTCCCGACGATTTTTGTGCGAGATGGGATCTGTCATAAGGACCATGCAGTTTCAGAACTCGGTATATTCGGTGCTTACTTAAGGTATGCAACTTACAACTTCATTGGGATTCACTGAGGGTAACCAACTAATTCTATTGCTCTAAATATATAGTAATTTTTTTCTTTTCAGTCAACCATGTTGTGATTTTGGGAATTCTGGTTGGAGTATACTTTTAAATAAAGTATTTAGTAGCGGTGTAAACCGAAACTAAGAAATAGAGAAAATTGGTTTGGCGTTCTAACTCTGTAGTGGATTAGTTTGATTTGGATATATTTTGTTGATTTGGGTTGGTCAAGCAGTTGAACTTCTAAAAAGTAGTTAATTTCAGTGTCAACCGACCCAGTTACACTAACGAGACTGATAAGTGAACTACTGTCTTTTATCTCATGCCTTTTCCTTTAAGCATTATTATTATTAGTTTTTGTTCAACAATTGTGAGATGGAGATCAAATCATCAACCTTTGACATGGAAATTGATTCCTTGTCTATTGAGTTATGCTTGGATTTTTGTTGCTTCTTTTCTTCTATAAACTGAAAAACCAATAAACTTACACTAATGATACTAAGATATGAAGTAATTTATATTAGCTCAAGTCCTTTTCTTTATGCATTATTATCGATACTTTTGTTCAACAATTGTGGGGTGGGGACCGAATCATCAACCTTAGAGATGTTAATTGGTTCTTTGTTCATAAATCTATGCTTGGATTGATTTAATTTTTCTCTTTTTGTAATATTTTTGAACTTCATATGATCAGGAACAAGGACAAAGTAATTGTAAATCAGCAGACTGGTTACTTGATGAGGACAAAAGTATCTTCATCAAATGAGGGTGGTGTTGCTGCTGGATTTGCCGTCTTGGATAGCATTTACTTAACTTGATTCTCTTTAATTACCCAATTCTCTCCATATTACAAATCCATAATCTTTATTATATACTTTGCTTGACAACAGGTTTTGTTGCCAGTTCTTCCTTTGTATAAGCCTCTATAAAAAAACTATATGGAATTTGATGATTTTAAATAATCCTAAGATAATCCAAATTTATAATTACAAATTATTCTGACCTCAAAGGCCCCAAACAACTGATATAATTTATTGTTATT

mRNA sequence

GGAAAACACTAGCAGAAATCGATAGAGAGGGAGTACTAAGATCTGATGGAACACTCGTTGTGTAAGGATGGCCAAGCAATTGCTGTTGTGTACTTCAGGGCTGGATATACGCCTAGGGACTATCCGTCTGAATCGGAATGGAGTGCAAGGTTACTTATGGAGCAGTCATCTGCAGTCAAATGCCCATCCATATCTTATCATTTAGTAGGCACCAAGAAAATTCAACAGGAACTTGCAAGACCAAATGTTCTTGAAAGGTTCCTTGACAGTGAAGAGGATATTGCCAAGATAAGAAAATGTTTTGCAGGGTTGTGGAGTTTAGATGACAAAGATATAGTTAAAAAAGCCATTGAAACACCGGAACTATTCGTTATGAAGCCACAAAGAGAAGGCGGAGGGAATAATATTTATGGAGATGATGTGAGGGAAGCCCTTCTAAAATTGCAGGAGGAAGGGAGTGAAGAAGATGGTGCTTACATCCTTATGCAGAGGATATTCCCATCTGTCTTCCCGACGATTTTTGTGCGAGATGGGATCTGTCATAAGGACCATGCAGTTTCAGAACTCGGTATATTCGGTGCTTACTTAAGGAACAAGGACAAAGTAATTGTAAATCAGCAGACTGGTTACTTGATGAGGACAAAAGTATCTTCATCAAATGAGGGTGGTGTTGCTGCTGGATTTGCCGTCTTGGATAGCATTTACTTAACTTGATTCTCTTTAATTACCCAATTCTCTCCATATTACAAATCCATAATCTTTATTATATACTTTGCTTGACAACAGGTTTTGTTGCCAGTTCTTCCTTTGTATAAGCCTCTATAAAAAAACTATATGGAATTTGATGATTTTAAATAATCCTAAGATAATCCAAATTTATAATTACAAATTATTCTGACCTCAAAGGCCCCAAACAACTGATATAATTTATTGTTATT

Coding sequence (CDS)

ATGGAACACTCGTTGTGTAAGGATGGCCAAGCAATTGCTGTTGTGTACTTCAGGGCTGGATATACGCCTAGGGACTATCCGTCTGAATCGGAATGGAGTGCAAGGTTACTTATGGAGCAGTCATCTGCAGTCAAATGCCCATCCATATCTTATCATTTAGTAGGCACCAAGAAAATTCAACAGGAACTTGCAAGACCAAATGTTCTTGAAAGGTTCCTTGACAGTGAAGAGGATATTGCCAAGATAAGAAAATGTTTTGCAGGGTTGTGGAGTTTAGATGACAAAGATATAGTTAAAAAAGCCATTGAAACACCGGAACTATTCGTTATGAAGCCACAAAGAGAAGGCGGAGGGAATAATATTTATGGAGATGATGTGAGGGAAGCCCTTCTAAAATTGCAGGAGGAAGGGAGTGAAGAAGATGGTGCTTACATCCTTATGCAGAGGATATTCCCATCTGTCTTCCCGACGATTTTTGTGCGAGATGGGATCTGTCATAAGGACCATGCAGTTTCAGAACTCGGTATATTCGGTGCTTACTTAAGGAACAAGGACAAAGTAATTGTAAATCAGCAGACTGGTTACTTGATGAGGACAAAAGTATCTTCATCAAATGAGGGTGGTGTTGCTGCTGGATTTGCCGTCTTGGATAGCATTTACTTAACTTGA

Protein sequence

MEHSLCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYLT
Homology
BLAST of Cmc02g0038701 vs. NCBI nr
Match: XP_008450698.1 (PREDICTED: glutathione synthetase, chloroplastic [Cucumis melo])

HSP 1 Score: 430.6 bits (1106), Expect = 8.0e-117
Identity = 212/219 (96.80%), Postives = 216/219 (98.63%), Query Frame = 0

Query: 4   SLCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQEL 63
           +L  DGQAIAVVYFRAGYTPRDYPSESEWSARLLME SSAVKCPSISYHLVGTKKIQQEL
Sbjct: 333 TLVVDGQAIAVVYFRAGYTPRDYPSESEWSARLLMELSSAVKCPSISYHLVGTKKIQQEL 392

Query: 64  ARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYG 123
           ARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYG
Sbjct: 393 ARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYG 452

Query: 124 DDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRN 183
           DDVREALLKLQ+EGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRN
Sbjct: 453 DDVREALLKLQKEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRN 512

Query: 184 KDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 223
           KDKVIVN+Q+GYLMRTKVSSSNEGGVAAGFAVLDSIYLT
Sbjct: 513 KDKVIVNEQSGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 551

BLAST of Cmc02g0038701 vs. NCBI nr
Match: ADM53349.1 (glutathione synthetase [Cucumis sativus])

HSP 1 Score: 424.1 bits (1089), Expect = 7.5e-115
Identity = 208/218 (95.41%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 5   LCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQELA 64
           L  DGQAIAVVYFRAGYTPRDYPSESEW ARLLMEQSSAVKCPSISYHLVGTKKIQQELA
Sbjct: 255 LIVDGQAIAVVYFRAGYTPRDYPSESEWRARLLMEQSSAVKCPSISYHLVGTKKIQQELA 314

Query: 65  RPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYGD 124
           RPNVLERFLDSE+DIAKIRKCFAGLWSLDD+DIVKKAIETPELFVMKPQREGGGNNIYGD
Sbjct: 315 RPNVLERFLDSEDDIAKIRKCFAGLWSLDDQDIVKKAIETPELFVMKPQREGGGNNIYGD 374

Query: 125 DVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRNK 184
           DVREALL+LQ+EGSEEDGAYILMQRIFPSV PTIFVRDGICHKDHAVSELGIFGAYLRNK
Sbjct: 375 DVREALLRLQKEGSEEDGAYILMQRIFPSVSPTIFVRDGICHKDHAVSELGIFGAYLRNK 434

Query: 185 DKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 223
           DKVIVN+Q+GYLMRTKVSSSNEGGVAAGFAVLDSIYLT
Sbjct: 435 DKVIVNEQSGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 472

BLAST of Cmc02g0038701 vs. NCBI nr
Match: XP_004135633.1 (glutathione synthetase, chloroplastic [Cucumis sativus])

HSP 1 Score: 422.2 bits (1084), Expect = 2.8e-114
Identity = 207/218 (94.95%), Postives = 213/218 (97.71%), Query Frame = 0

Query: 5   LCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQELA 64
           L  DGQAIAVVYFRAGYTPRDYPSESEW ARLLMEQSSAVKCPSISYHLVGTKKIQQELA
Sbjct: 330 LIVDGQAIAVVYFRAGYTPRDYPSESEWRARLLMEQSSAVKCPSISYHLVGTKKIQQELA 389

Query: 65  RPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYGD 124
           RPNVLERFLDSE+DIAKIRKCFAGLWSLDD+DIV KAIETPELFVMKPQREGGGNNIYGD
Sbjct: 390 RPNVLERFLDSEDDIAKIRKCFAGLWSLDDQDIVNKAIETPELFVMKPQREGGGNNIYGD 449

Query: 125 DVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRNK 184
           DVREALL+LQ+EGSEEDGAYILMQRIFPSV PTIFVRDGICHKDHAVSELGIFGAYLRNK
Sbjct: 450 DVREALLRLQKEGSEEDGAYILMQRIFPSVSPTIFVRDGICHKDHAVSELGIFGAYLRNK 509

Query: 185 DKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 223
           DKVIVN+Q+GYLMRTKVSSSNEGGVAAGFAVLDSIYLT
Sbjct: 510 DKVIVNEQSGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 547

BLAST of Cmc02g0038701 vs. NCBI nr
Match: XP_038877693.1 (glutathione synthetase, chloroplastic [Benincasa hispida])

HSP 1 Score: 421.0 bits (1081), Expect = 6.3e-114
Identity = 205/219 (93.61%), Postives = 213/219 (97.26%), Query Frame = 0

Query: 4   SLCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQEL 63
           +L  DGQAIAVVYFRAGYTPRDYPSESEW ARLLMEQSSAVKCPSISYHLVGTKKIQQEL
Sbjct: 327 TLLVDGQAIAVVYFRAGYTPRDYPSESEWRARLLMEQSSAVKCPSISYHLVGTKKIQQEL 386

Query: 64  ARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYG 123
           A+PNVLERFLDS+EDIAKIRKCFAGLWSLDDKDIVKKAIETP+LFVMKPQREGGGNNIYG
Sbjct: 387 AKPNVLERFLDSKEDIAKIRKCFAGLWSLDDKDIVKKAIETPDLFVMKPQREGGGNNIYG 446

Query: 124 DDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRN 183
           DDVREALL+LQ+EGSEED AYILMQRIFPSVFPTIFVRDGICHKDHA+SELGIFGAYLRN
Sbjct: 447 DDVREALLRLQKEGSEEDAAYILMQRIFPSVFPTIFVRDGICHKDHAISELGIFGAYLRN 506

Query: 184 KDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 223
           KDKVIVN  +GYLMRTKVSSSNEGGVAAGFAVLDSIYLT
Sbjct: 507 KDKVIVNDHSGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 545

BLAST of Cmc02g0038701 vs. NCBI nr
Match: XP_023537149.1 (glutathione synthetase, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 402.9 bits (1034), Expect = 1.8e-108
Identity = 193/219 (88.13%), Postives = 209/219 (95.43%), Query Frame = 0

Query: 4   SLCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQEL 63
           +L  DGQA+AVVYFRAGYTP DYPSE+EW+ARLL+EQSSAVKCPSISYHL GTKKIQQEL
Sbjct: 321 TLLVDGQAVAVVYFRAGYTPIDYPSETEWAARLLVEQSSAVKCPSISYHLAGTKKIQQEL 380

Query: 64  ARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYG 123
           A+PNVLERFLD++EDIAKIRKCFAGLWSLDDKDIVKKAIE P+LFVMKPQREGGGNNIYG
Sbjct: 381 AKPNVLERFLDNKEDIAKIRKCFAGLWSLDDKDIVKKAIEAPDLFVMKPQREGGGNNIYG 440

Query: 124 DDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRN 183
           DDVR+ L++LQ+EG+ ED AYILMQRIFPSV+PTIFVRDGICHKDHA+SELGIFGAYLRN
Sbjct: 441 DDVRKTLVRLQKEGNVEDAAYILMQRIFPSVYPTIFVRDGICHKDHAISELGIFGAYLRN 500

Query: 184 KDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 223
           KDKVIVN Q GYLMRTKVSSSNEGGVAAGFAVLDSIYLT
Sbjct: 501 KDKVIVNDQCGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 539

BLAST of Cmc02g0038701 vs. ExPASy Swiss-Prot
Match: O23732 (Glutathione synthetase, chloroplastic OS=Brassica juncea OX=3707 GN=GSH2 PE=2 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 6.0e-99
Identity = 168/218 (77.06%), Postives = 198/218 (90.83%), Query Frame = 0

Query: 4   SLCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQEL 63
           +L   GQA++VVY+R+GYTPRDYPSESEW+ARLL+EQSSAVKCPSI+YHL GTKKIQQEL
Sbjct: 312 TLTVGGQAVSVVYYRSGYTPRDYPSESEWNARLLIEQSSAVKCPSIAYHLAGTKKIQQEL 371

Query: 64  ARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYG 123
           A+P VLERF+D+++DIAK+RKCFAGLWSLDD +I+KKAIE PELFVMKPQREGGGNNIYG
Sbjct: 372 AKPGVLERFMDNKDDIAKLRKCFAGLWSLDDPEIIKKAIEKPELFVMKPQREGGGNNIYG 431

Query: 124 DDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRN 183
           DDVRE LL+LQ EG EE+ AYILMQRIFP V     +RDG+ HKD A+SELG++GAYLRN
Sbjct: 432 DDVRENLLRLQREGEEENAAYILMQRIFPKVSNVFLLRDGVYHKDQAISELGVYGAYLRN 491

Query: 184 KDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYL 222
           K++VI+N+ +GYLMRTKVSSS+EGGVAAG+AVLDSIYL
Sbjct: 492 KERVIINEHSGYLMRTKVSSSDEGGVAAGYAVLDSIYL 529

BLAST of Cmc02g0038701 vs. ExPASy Swiss-Prot
Match: O22494 (Glutathione synthetase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=GSH2 PE=2 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 2.8e-96
Identity = 167/218 (76.61%), Postives = 193/218 (88.53%), Query Frame = 0

Query: 4   SLCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQEL 63
           +L  DGQA+AV+YFRAGY P DY SESEW ARLLMEQS AVKCPSISYHL G+KKIQQEL
Sbjct: 328 TLVVDGQAVAVIYFRAGYAPSDYHSESEWKARLLMEQSRAVKCPSISYHLAGSKKIQQEL 387

Query: 64  ARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYG 123
           A+PNVLERFL++++DIAK+RKCFAGLWSLD+ DIVK AIE PEL+VMKPQREGGGNNIYG
Sbjct: 388 AKPNVLERFLENKDDIAKLRKCFAGLWSLDESDIVKDAIERPELYVMKPQREGGGNNIYG 447

Query: 124 DDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRN 183
           +DVR ALLKLQ+EG+  D AYILMQRIFP +  +I +R+GI HK+  +SELGI+G YLRN
Sbjct: 448 EDVRGALLKLQKEGTGSDAAYILMQRIFPKISHSILMREGISHKEETISELGIYGTYLRN 507

Query: 184 KDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYL 222
           K +V++NQQ GYLMRTKVSSS+EGGVAAGFAVLDSIYL
Sbjct: 508 KTEVLINQQAGYLMRTKVSSSDEGGVAAGFAVLDSIYL 545

BLAST of Cmc02g0038701 vs. ExPASy Swiss-Prot
Match: P46416 (Glutathione synthetase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GSH2 PE=2 SV=3)

HSP 1 Score: 350.9 bits (899), Expect = 1.1e-95
Identity = 165/220 (75.00%), Postives = 196/220 (89.09%), Query Frame = 0

Query: 2   EHSLCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQ 61
           + +L   GQA+AVVYFR+GYTP D+PSESEW+ARLL+E+SSAVKCPSI+YHL G+KKIQQ
Sbjct: 319 DETLIVGGQAVAVVYFRSGYTPNDHPSESEWNARLLIEESSAVKCPSIAYHLTGSKKIQQ 378

Query: 62  ELARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNI 121
           ELA+P VLERFLD++EDIAK+RKCFAGLWSLDD +IVK+AIE P LFVMKPQREGGGNNI
Sbjct: 379 ELAKPGVLERFLDNKEDIAKLRKCFAGLWSLDDSEIVKQAIEKPGLFVMKPQREGGGNNI 438

Query: 122 YGDDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYL 181
           YGDDVRE LL+LQ+EG E + AYILMQRIFP V     VR+G+ HK  A+SELG++GAYL
Sbjct: 439 YGDDVRENLLRLQKEGEEGNAAYILMQRIFPKVSNMFLVREGVYHKHQAISELGVYGAYL 498

Query: 182 RNKDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYL 222
           R+KD+VIVN+Q+GYLMRTK++SS+EGGVAAGF VLDSIYL
Sbjct: 499 RSKDEVIVNEQSGYLMRTKIASSDEGGVAAGFGVLDSIYL 538

BLAST of Cmc02g0038701 vs. ExPASy Swiss-Prot
Match: P35668 (Glutathione synthetase OS=Xenopus laevis OX=8355 GN=gss PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 8.2e-56
Identity = 114/227 (50.22%), Postives = 156/227 (68.72%), Query Frame = 0

Query: 2   EHSLCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQ 61
           E  L  DG  +AV YFR GY P+DY +E +W ARL++E+S AVKCP +   LVGTKK+QQ
Sbjct: 251 ERHLFIDGYEVAVAYFRTGYVPQDY-TEQDWEARLMLERSRAVKCPDVPTQLVGTKKVQQ 310

Query: 62  ELARPNVLERFL-DSEEDIAKIRKCFAGLWSLD----DKDIVKKAIETPELFVMKPQREG 121
           EL+RP +LE+FL D  E +A+IR+ F GL+SLD      + V+ A+  P+ FV+KPQREG
Sbjct: 311 ELSRPQILEKFLPDKPEAVARIRETFTGLYSLDIGEEGDEAVRVALANPDQFVLKPQREG 370

Query: 122 GGNNIYGDDVREALLKLQE-EGSEEDGAYILMQRIFPSVFPTIFVR-DGICHKDHAVSEL 181
           GGNN+YG++++E   KLQE + SEE  +YILM +I P       +R  G       +SEL
Sbjct: 371 GGNNLYGEELKE---KLQECKDSEERTSYILMDKINPKPLKNCLLRAGGRVQISECISEL 430

Query: 182 GIFGAYLRNKDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYL 222
           G+FG Y+R++D++I   Q G+L+RTK    ++GGVAAG AVLD+ YL
Sbjct: 431 GMFGVYVRHRDQMIYYDQVGHLLRTKAIEHSDGGVAAGVAVLDNPYL 473

BLAST of Cmc02g0038701 vs. ExPASy Swiss-Prot
Match: Q54E83 (Glutathione synthetase OS=Dictyostelium discoideum OX=44689 GN=gshB PE=3 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 6.9e-55
Identity = 113/222 (50.90%), Postives = 155/222 (69.82%), Query Frame = 0

Query: 5   LCKDGQAIAVVYFRAGYTPRDYPSE--SEWSARLLMEQSSAVKCPSISYHLVGTKKIQQE 64
           L  DG  I+V Y+RAGYTP DY S    EW ARLL+E+S A+KCP+I++HLVG KKIQQ 
Sbjct: 254 LIVDGMEISVAYYRAGYTPNDYTSSGGDEWKARLLIERSLAIKCPTIAHHLVGVKKIQQV 313

Query: 65  LARPNVLERFLDSE-EDIAKIRKCFAGLWSLDDKDI----VKKAIETPELFVMKPQREGG 124
           LA+P VLE+F++++ E + ++++ F GL+SL  +DI    VK+AIE+P+ +VMKPQREGG
Sbjct: 314 LAQPGVLEKFINNDKESLQRVKRSFTGLYSLSKEDIDMSVVKEAIESPQNYVMKPQREGG 373

Query: 125 GNNIYGDDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIF 184
           GNNIY D V  AL  +    SEE  +YILM +I    F T  VRD    +   + ELGI+
Sbjct: 374 GNNIYNDQVAIALKSM---SSEELSSYILMDKIMSKSFKTHVVRDRQLLEIEGLYELGIY 433

Query: 185 GAYLRN-KDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDS 219
             ++ N  D +++N+Q G L+RTK ++S+E GVAAGF +LDS
Sbjct: 434 SVFISNGDDDIVLNKQAGILLRTKTANSDEVGVAAGFGLLDS 472

BLAST of Cmc02g0038701 vs. ExPASy TrEMBL
Match: A0A1S3BQG6 (Glutathione synthetase OS=Cucumis melo OX=3656 GN=LOC103492200 PE=3 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 3.9e-117
Identity = 212/219 (96.80%), Postives = 216/219 (98.63%), Query Frame = 0

Query: 4   SLCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQEL 63
           +L  DGQAIAVVYFRAGYTPRDYPSESEWSARLLME SSAVKCPSISYHLVGTKKIQQEL
Sbjct: 333 TLVVDGQAIAVVYFRAGYTPRDYPSESEWSARLLMELSSAVKCPSISYHLVGTKKIQQEL 392

Query: 64  ARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYG 123
           ARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYG
Sbjct: 393 ARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYG 452

Query: 124 DDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRN 183
           DDVREALLKLQ+EGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRN
Sbjct: 453 DDVREALLKLQKEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRN 512

Query: 184 KDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 223
           KDKVIVN+Q+GYLMRTKVSSSNEGGVAAGFAVLDSIYLT
Sbjct: 513 KDKVIVNEQSGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 551

BLAST of Cmc02g0038701 vs. ExPASy TrEMBL
Match: K9JF36 (Glutathione synthetase OS=Cucumis sativus OX=3659 GN=GSH2 PE=2 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 3.6e-115
Identity = 208/218 (95.41%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 5   LCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQELA 64
           L  DGQAIAVVYFRAGYTPRDYPSESEW ARLLMEQSSAVKCPSISYHLVGTKKIQQELA
Sbjct: 255 LIVDGQAIAVVYFRAGYTPRDYPSESEWRARLLMEQSSAVKCPSISYHLVGTKKIQQELA 314

Query: 65  RPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYGD 124
           RPNVLERFLDSE+DIAKIRKCFAGLWSLDD+DIVKKAIETPELFVMKPQREGGGNNIYGD
Sbjct: 315 RPNVLERFLDSEDDIAKIRKCFAGLWSLDDQDIVKKAIETPELFVMKPQREGGGNNIYGD 374

Query: 125 DVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRNK 184
           DVREALL+LQ+EGSEEDGAYILMQRIFPSV PTIFVRDGICHKDHAVSELGIFGAYLRNK
Sbjct: 375 DVREALLRLQKEGSEEDGAYILMQRIFPSVSPTIFVRDGICHKDHAVSELGIFGAYLRNK 434

Query: 185 DKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 223
           DKVIVN+Q+GYLMRTKVSSSNEGGVAAGFAVLDSIYLT
Sbjct: 435 DKVIVNEQSGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 472

BLAST of Cmc02g0038701 vs. ExPASy TrEMBL
Match: A0A0A0LW32 (Glutathione synthetase OS=Cucumis sativus OX=3659 GN=Csa_1G571280 PE=3 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.4e-114
Identity = 207/218 (94.95%), Postives = 213/218 (97.71%), Query Frame = 0

Query: 5   LCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQELA 64
           L  DGQAIAVVYFRAGYTPRDYPSESEW ARLLMEQSSAVKCPSISYHLVGTKKIQQELA
Sbjct: 330 LIVDGQAIAVVYFRAGYTPRDYPSESEWRARLLMEQSSAVKCPSISYHLVGTKKIQQELA 389

Query: 65  RPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYGD 124
           RPNVLERFLDSE+DIAKIRKCFAGLWSLDD+DIV KAIETPELFVMKPQREGGGNNIYGD
Sbjct: 390 RPNVLERFLDSEDDIAKIRKCFAGLWSLDDQDIVNKAIETPELFVMKPQREGGGNNIYGD 449

Query: 125 DVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRNK 184
           DVREALL+LQ+EGSEEDGAYILMQRIFPSV PTIFVRDGICHKDHAVSELGIFGAYLRNK
Sbjct: 450 DVREALLRLQKEGSEEDGAYILMQRIFPSVSPTIFVRDGICHKDHAVSELGIFGAYLRNK 509

Query: 185 DKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 223
           DKVIVN+Q+GYLMRTKVSSSNEGGVAAGFAVLDSIYLT
Sbjct: 510 DKVIVNEQSGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 547

BLAST of Cmc02g0038701 vs. ExPASy TrEMBL
Match: A0A6J1KK27 (Glutathione synthetase OS=Cucurbita maxima OX=3661 GN=LOC111495981 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.5e-108
Identity = 193/219 (88.13%), Postives = 207/219 (94.52%), Query Frame = 0

Query: 4   SLCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQEL 63
           +L  DGQA+AVVY+RAGYTP DYPSE EW ARLLMEQSSAVKCPSISYHLVGTKKIQQEL
Sbjct: 240 TLLVDGQAVAVVYYRAGYTPTDYPSELEWKARLLMEQSSAVKCPSISYHLVGTKKIQQEL 299

Query: 64  ARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYG 123
           A+PNVLERFLD++EDIAKIRKCFAGLWSLDDKDIVKKAIE P+LFVMKPQREGGGNNIYG
Sbjct: 300 AQPNVLERFLDNKEDIAKIRKCFAGLWSLDDKDIVKKAIEAPDLFVMKPQREGGGNNIYG 359

Query: 124 DDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRN 183
           DDVR+ L++LQ+EG+ ED AYILMQRIFPSV+PTIFVRDGICHKDHA+SELGIFGAYLRN
Sbjct: 360 DDVRKTLVRLQKEGNVEDAAYILMQRIFPSVYPTIFVRDGICHKDHAISELGIFGAYLRN 419

Query: 184 KDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 223
           KDKVIVN Q GYLM TKVSSSNEGGVAAGFAVLDSIYLT
Sbjct: 420 KDKVIVNDQCGYLMTTKVSSSNEGGVAAGFAVLDSIYLT 458

BLAST of Cmc02g0038701 vs. ExPASy TrEMBL
Match: A0A6J1KP26 (Glutathione synthetase OS=Cucurbita maxima OX=3661 GN=LOC111495981 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.5e-108
Identity = 193/219 (88.13%), Postives = 207/219 (94.52%), Query Frame = 0

Query: 4   SLCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQEL 63
           +L  DGQA+AVVY+RAGYTP DYPSE EW ARLLMEQSSAVKCPSISYHLVGTKKIQQEL
Sbjct: 338 TLLVDGQAVAVVYYRAGYTPTDYPSELEWKARLLMEQSSAVKCPSISYHLVGTKKIQQEL 397

Query: 64  ARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNIYG 123
           A+PNVLERFLD++EDIAKIRKCFAGLWSLDDKDIVKKAIE P+LFVMKPQREGGGNNIYG
Sbjct: 398 AQPNVLERFLDNKEDIAKIRKCFAGLWSLDDKDIVKKAIEAPDLFVMKPQREGGGNNIYG 457

Query: 124 DDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYLRN 183
           DDVR+ L++LQ+EG+ ED AYILMQRIFPSV+PTIFVRDGICHKDHA+SELGIFGAYLRN
Sbjct: 458 DDVRKTLVRLQKEGNVEDAAYILMQRIFPSVYPTIFVRDGICHKDHAISELGIFGAYLRN 517

Query: 184 KDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYLT 223
           KDKVIVN Q GYLM TKVSSSNEGGVAAGFAVLDSIYLT
Sbjct: 518 KDKVIVNDQCGYLMTTKVSSSNEGGVAAGFAVLDSIYLT 556

BLAST of Cmc02g0038701 vs. TAIR 10
Match: AT5G27380.1 (glutathione synthetase 2 )

HSP 1 Score: 350.9 bits (899), Expect = 7.5e-97
Identity = 165/220 (75.00%), Postives = 196/220 (89.09%), Query Frame = 0

Query: 2   EHSLCKDGQAIAVVYFRAGYTPRDYPSESEWSARLLMEQSSAVKCPSISYHLVGTKKIQQ 61
           + +L   GQA+AVVYFR+GYTP D+PSESEW+ARLL+E+SSAVKCPSI+YHL G+KKIQQ
Sbjct: 319 DETLIVGGQAVAVVYFRSGYTPNDHPSESEWNARLLIEESSAVKCPSIAYHLTGSKKIQQ 378

Query: 62  ELARPNVLERFLDSEEDIAKIRKCFAGLWSLDDKDIVKKAIETPELFVMKPQREGGGNNI 121
           ELA+P VLERFLD++EDIAK+RKCFAGLWSLDD +IVK+AIE P LFVMKPQREGGGNNI
Sbjct: 379 ELAKPGVLERFLDNKEDIAKLRKCFAGLWSLDDSEIVKQAIEKPGLFVMKPQREGGGNNI 438

Query: 122 YGDDVREALLKLQEEGSEEDGAYILMQRIFPSVFPTIFVRDGICHKDHAVSELGIFGAYL 181
           YGDDVRE LL+LQ+EG E + AYILMQRIFP V     VR+G+ HK  A+SELG++GAYL
Sbjct: 439 YGDDVRENLLRLQKEGEEGNAAYILMQRIFPKVSNMFLVREGVYHKHQAISELGVYGAYL 498

Query: 182 RNKDKVIVNQQTGYLMRTKVSSSNEGGVAAGFAVLDSIYL 222
           R+KD+VIVN+Q+GYLMRTK++SS+EGGVAAGF VLDSIYL
Sbjct: 499 RSKDEVIVNEQSGYLMRTKIASSDEGGVAAGFGVLDSIYL 538

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008450698.18.0e-11796.80PREDICTED: glutathione synthetase, chloroplastic [Cucumis melo][more]
ADM53349.17.5e-11595.41glutathione synthetase [Cucumis sativus][more]
XP_004135633.12.8e-11494.95glutathione synthetase, chloroplastic [Cucumis sativus][more]
XP_038877693.16.3e-11493.61glutathione synthetase, chloroplastic [Benincasa hispida][more]
XP_023537149.11.8e-10888.13glutathione synthetase, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
O237326.0e-9977.06Glutathione synthetase, chloroplastic OS=Brassica juncea OX=3707 GN=GSH2 PE=2 SV... [more]
O224942.8e-9676.61Glutathione synthetase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=GSH2 PE... [more]
P464161.1e-9575.00Glutathione synthetase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GSH2 PE... [more]
P356688.2e-5650.22Glutathione synthetase OS=Xenopus laevis OX=8355 GN=gss PE=2 SV=1[more]
Q54E836.9e-5550.90Glutathione synthetase OS=Dictyostelium discoideum OX=44689 GN=gshB PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BQG63.9e-11796.80Glutathione synthetase OS=Cucumis melo OX=3656 GN=LOC103492200 PE=3 SV=1[more]
K9JF363.6e-11595.41Glutathione synthetase OS=Cucumis sativus OX=3659 GN=GSH2 PE=2 SV=1[more]
A0A0A0LW321.4e-11494.95Glutathione synthetase OS=Cucumis sativus OX=3659 GN=Csa_1G571280 PE=3 SV=1[more]
A0A6J1KK272.5e-10888.13Glutathione synthetase OS=Cucurbita maxima OX=3661 GN=LOC111495981 PE=3 SV=1[more]
A0A6J1KP262.5e-10888.13Glutathione synthetase OS=Cucurbita maxima OX=3661 GN=LOC111495981 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G27380.17.5e-9775.00glutathione synthetase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004887Glutathione synthase, substrate-binding domainPFAMPF03199GSH_synthasecoord: 9..54
e-value: 8.4E-20
score: 71.2
IPR037013Glutathione synthase, substrate-binding domain superfamilyGENE3D3.40.50.1760coord: 1..44
e-value: 6.4E-20
score: 73.7
IPR014709Glutathione synthase, C-terminal, eukaryoticGENE3D3.30.1490.50coord: 89..154
e-value: 1.5E-28
score: 100.4
IPR005615Glutathione synthaseTIGRFAMTIGR01986TIGR01986coord: 8..221
e-value: 1.6E-104
score: 348.0
IPR005615Glutathione synthasePFAMPF03917GSH_synth_ATPcoord: 55..221
e-value: 1.6E-77
score: 261.0
IPR005615Glutathione synthasePANTHERPTHR11130GLUTATHIONE SYNTHETASEcoord: 4..222
NoneNo IPR availableGENE3D3.30.470.20coord: 162..221
e-value: 3.0E-18
score: 67.7
NoneNo IPR availablePIRSRPIRSR001558-1PIRSR001558-1coord: 3..221
e-value: 2.7E-111
score: 369.1
NoneNo IPR availablePIRSRPIRSR001558-2PIRSR001558-2coord: 3..221
e-value: 1.2E-110
score: 367.0
NoneNo IPR availablePANTHERPTHR11130:SF3GLUTATHIONE SYNTHETASEcoord: 4..222
NoneNo IPR availableSUPERFAMILY56059Glutathione synthetase ATP-binding domain-likecoord: 11..221
IPR014042Glutathione synthase, alpha-helicalGENE3D1.10.1080.10Glutathione Synthetase; Chain A, domain 3coord: 45..88
e-value: 1.2E-8
score: 36.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc02g0038701.1Cmc02g0038701.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006750 glutathione biosynthetic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005829 cytosol
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0043295 glutathione binding
molecular_function GO:0004363 glutathione synthase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0016874 ligase activity