Cmc01g0023381 (gene) Melon (Charmono) v1.1

Overview
NameCmc01g0023381
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionReverse transcriptase
LocationCMiso1.1chr01: 23157562 .. 23157894 (+)
RNA-Seq ExpressionCmc01g0023381
SyntenyCmc01g0023381
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACCGATGGAACTGAGAGAGCTAAAGTCGCAGTTGCAGGAATTAGTGGATACGGGATTTATCCGACCTAGTGCATCTCCGTGGGGTGCACCGGTGTTGTTTGTTAAAAAGAAGGATGGTACATTAAGGTTATGTATTGACTACCGGCAGTTAAATAAGGTAACAATACATAACAAATATCCTTTGCCCCGAATAGATGATTTGTTTGATCAGCTTCATGGTGCCTCTGTATTCTCTAAGATTGACTTGAGATCAGGTTATCATCAGTTGAAAGTTAAAGAGTCAGATATCCTGAAAAACAGCCTTTCGAACGAGATATGGGCATTATGA

mRNA sequence

ATGGCACCGATGGAACTGAGAGAGCTAAAGTCGCAGTTGCAGGAATTAGTGGATACGGGATTTATCCGACCTAGTGCATCTCCGTGGGGTGCACCGGTGTTGTTTGTTAAAAAGAAGGATGGTACATTAAGGTTATGTATTGACTACCGGCAGTTAAATAAGGTAACAATACATAACAAATATCCTTTGCCCCGAATAGATGATTTGTTTGATCAGCTTCATGGTGCCTCTGTATTCTCTAAGATTGACTTGAGATCAGGTTATCATCAGTTGAAAGTTAAAGAGTCAGATATCCTGAAAAACAGCCTTTCGAACGAGATATGGGCATTATGA

Coding sequence (CDS)

ATGGCACCGATGGAACTGAGAGAGCTAAAGTCGCAGTTGCAGGAATTAGTGGATACGGGATTTATCCGACCTAGTGCATCTCCGTGGGGTGCACCGGTGTTGTTTGTTAAAAAGAAGGATGGTACATTAAGGTTATGTATTGACTACCGGCAGTTAAATAAGGTAACAATACATAACAAATATCCTTTGCCCCGAATAGATGATTTGTTTGATCAGCTTCATGGTGCCTCTGTATTCTCTAAGATTGACTTGAGATCAGGTTATCATCAGTTGAAAGTTAAAGAGTCAGATATCCTGAAAAACAGCCTTTCGAACGAGATATGGGCATTATGA

Protein sequence

MAPMELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNKYPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKESDILKNSLSNEIWAL
Homology
BLAST of Cmc01g0023381 vs. NCBI nr
Match: TYK18080.1 (hypothetical protein E5676_scaffold306G004160 [Cucumis melo var. makuwa])

HSP 1 Score: 203.0 bits (515), Expect = 1.3e-48
Identity = 97/102 (95.10%), Postives = 99/102 (97.06%), Query Frame = 0

Query: 1   MAPMELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 60
           MAPME+RELKSQLQELVD GFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK
Sbjct: 179 MAPMEMRELKSQLQELVDMGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 238

Query: 61  YPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKESDILKNS 103
           YPLPRIDDLFDQ HGASVFSKIDLRSGYHQLKVKESDILK +
Sbjct: 239 YPLPRIDDLFDQHHGASVFSKIDLRSGYHQLKVKESDILKTA 280

BLAST of Cmc01g0023381 vs. NCBI nr
Match: KAA0042141.1 (hypothetical protein E6C27_scaffold67G006300 [Cucumis melo var. makuwa])

HSP 1 Score: 203.0 bits (515), Expect = 1.3e-48
Identity = 97/102 (95.10%), Postives = 99/102 (97.06%), Query Frame = 0

Query: 1   MAPMELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 60
           MAPME+RELKSQLQELVD GFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK
Sbjct: 179 MAPMEMRELKSQLQELVDMGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 238

Query: 61  YPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKESDILKNS 103
           YPLPRIDDLFDQ HGASVFSKIDLRSGYHQLKVKESDILK +
Sbjct: 239 YPLPRIDDLFDQHHGASVFSKIDLRSGYHQLKVKESDILKTA 280

BLAST of Cmc01g0023381 vs. NCBI nr
Match: KAA0061889.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 188.3 bits (477), Expect = 3.4e-44
Identity = 88/102 (86.27%), Postives = 95/102 (93.14%), Query Frame = 0

Query: 1   MAPMELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 60
           +AP EL+ELK QLQELVD G+IRPS SPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK
Sbjct: 536 IAPSELKELKMQLQELVDKGYIRPSVSPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 595

Query: 61  YPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKESDILKNS 103
           YPLPRIDDLFDQL GA++FSKIDLRSGYHQLKV+ESDI K +
Sbjct: 596 YPLPRIDDLFDQLRGAALFSKIDLRSGYHQLKVRESDIAKTA 637

BLAST of Cmc01g0023381 vs. NCBI nr
Match: TYK27290.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 186.8 bits (473), Expect = 1.0e-43
Identity = 88/102 (86.27%), Postives = 94/102 (92.16%), Query Frame = 0

Query: 1   MAPMELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 60
           MAP EL+ELK QLQELVD G+IRPS SPWGAPVLFVKKKDGTLRLCIDYRQLNKVTI NK
Sbjct: 594 MAPSELKELKMQLQELVDKGYIRPSVSPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIRNK 653

Query: 61  YPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKESDILKNS 103
           YPLPRIDDLFDQL GA++FSKIDLRSGYHQLKV+ESDI K +
Sbjct: 654 YPLPRIDDLFDQLRGAALFSKIDLRSGYHQLKVRESDIAKTA 695

BLAST of Cmc01g0023381 vs. NCBI nr
Match: KAA0050527.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 186.8 bits (473), Expect = 1.0e-43
Identity = 88/102 (86.27%), Postives = 94/102 (92.16%), Query Frame = 0

Query: 1   MAPMELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 60
           MAP EL+ELK QLQELVD G+IRPS SPWGAPVLFVKKKDGTLRLCIDYRQLNKVTI NK
Sbjct: 326 MAPSELKELKMQLQELVDKGYIRPSVSPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIRNK 385

Query: 61  YPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKESDILKNS 103
           YPLPRIDDLFDQL GA++FSKIDLRSGYHQLKV+ESDI K +
Sbjct: 386 YPLPRIDDLFDQLRGATLFSKIDLRSGYHQLKVRESDIAKTA 427

BLAST of Cmc01g0023381 vs. ExPASy Swiss-Prot
Match: Q99315 (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 107.8 bits (268), Expect = 7.7e-23
Identity = 47/96 (48.96%), Postives = 67/96 (69.79%), Query Frame = 0

Query: 7   RELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNKYPLPRI 66
           +E+   +Q+L+D  FI PS SP  +PV+ V KKDGT RLC+DYR LNK TI + +PLPRI
Sbjct: 610 QEINKIVQKLLDNKFIVPSKSPCSSPVVLVPKKDGTFRLCVDYRTLNKATISDPFPLPRI 669

Query: 67  DDLFDQLHGASVFSKIDLRSGYHQLKVKESDILKNS 103
           D+L  ++  A +F+ +DL SGYHQ+ ++  D  K +
Sbjct: 670 DNLLSRIGNAQIFTTLDLHSGYHQIPMEPKDRYKTA 705

BLAST of Cmc01g0023381 vs. ExPASy Swiss-Prot
Match: Q7LHG5 (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 7.7e-23
Identity = 47/96 (48.96%), Postives = 67/96 (69.79%), Query Frame = 0

Query: 7   RELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNKYPLPRI 66
           +E+   +Q+L+D  FI PS SP  +PV+ V KKDGT RLC+DYR LNK TI + +PLPRI
Sbjct: 636 QEINKIVQKLLDNKFIVPSKSPCSSPVVLVPKKDGTFRLCVDYRTLNKATISDPFPLPRI 695

Query: 67  DDLFDQLHGASVFSKIDLRSGYHQLKVKESDILKNS 103
           D+L  ++  A +F+ +DL SGYHQ+ ++  D  K +
Sbjct: 696 DNLLSRIGNAQIFTTLDLHSGYHQIPMEPKDRYKTA 731

BLAST of Cmc01g0023381 vs. ExPASy Swiss-Prot
Match: P10394 (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 2.6e-18
Identity = 47/109 (43.12%), Postives = 69/109 (63.30%), Query Frame = 0

Query: 5   ELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDG------TLRLCIDYRQLNKVTIH 64
           ++ E+++Q+Q+L+    + PS S + +P+L V KK          RL IDYRQ+NK  + 
Sbjct: 326 QVEEIQAQVQKLIKDKIVEPSVSQYNSPLLLVPKKSSPNSDKKKWRLVIDYRQINKKLLA 385

Query: 65  NKYPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKES--DILKNSLSN 106
           +K+PLPRIDD+ DQL  A  FS +DL SG+HQ+++ E   DI   S SN
Sbjct: 386 DKFPLPRIDDILDQLGRAKYFSCLDLMSGFHQIELDEGSRDITSFSTSN 434

BLAST of Cmc01g0023381 vs. ExPASy Swiss-Prot
Match: Q9UR07 (Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-11 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 9.8e-18
Identity = 37/97 (38.14%), Postives = 66/97 (68.04%), Query Frame = 0

Query: 1   MAPMELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 60
           + P +++ +  ++ + + +G IR S +    PV+FV KK+GTLR+ +DY+ LNK    N 
Sbjct: 420 LPPGKMQAMNDEINQGLKSGIIRESKAINACPVMFVPKKEGTLRMVVDYKPLNKYVKPNI 479

Query: 61  YPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKESD 98
           YPLP I+ L  ++ G+++F+K+DL+S YH ++V++ D
Sbjct: 480 YPLPLIEQLLAKIQGSTIFTKLDLKSAYHLIRVRKGD 516

BLAST of Cmc01g0023381 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 9.8e-18
Identity = 37/97 (38.14%), Postives = 66/97 (68.04%), Query Frame = 0

Query: 1   MAPMELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 60
           + P +++ +  ++ + + +G IR S +    PV+FV KK+GTLR+ +DY+ LNK    N 
Sbjct: 420 LPPGKMQAMNDEINQGLKSGIIRESKAINACPVMFVPKKEGTLRMVVDYKPLNKYVKPNI 479

Query: 61  YPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKESD 98
           YPLP I+ L  ++ G+++F+K+DL+S YH ++V++ D
Sbjct: 480 YPLPLIEQLLAKIQGSTIFTKLDLKSAYHLIRVRKGD 516

BLAST of Cmc01g0023381 vs. ExPASy TrEMBL
Match: A0A5D3D2Y2 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G004160 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 6.5e-49
Identity = 97/102 (95.10%), Postives = 99/102 (97.06%), Query Frame = 0

Query: 1   MAPMELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 60
           MAPME+RELKSQLQELVD GFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK
Sbjct: 179 MAPMEMRELKSQLQELVDMGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 238

Query: 61  YPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKESDILKNS 103
           YPLPRIDDLFDQ HGASVFSKIDLRSGYHQLKVKESDILK +
Sbjct: 239 YPLPRIDDLFDQHHGASVFSKIDLRSGYHQLKVKESDILKTA 280

BLAST of Cmc01g0023381 vs. ExPASy TrEMBL
Match: A0A5A7TKQ0 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67G006300 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 6.5e-49
Identity = 97/102 (95.10%), Postives = 99/102 (97.06%), Query Frame = 0

Query: 1   MAPMELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 60
           MAPME+RELKSQLQELVD GFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK
Sbjct: 179 MAPMEMRELKSQLQELVDMGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 238

Query: 61  YPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKESDILKNS 103
           YPLPRIDDLFDQ HGASVFSKIDLRSGYHQLKVKESDILK +
Sbjct: 239 YPLPRIDDLFDQHHGASVFSKIDLRSGYHQLKVKESDILKTA 280

BLAST of Cmc01g0023381 vs. ExPASy TrEMBL
Match: A0A5A7V873 (DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G001390 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.7e-44
Identity = 88/102 (86.27%), Postives = 95/102 (93.14%), Query Frame = 0

Query: 1   MAPMELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 60
           +AP EL+ELK QLQELVD G+IRPS SPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK
Sbjct: 536 IAPSELKELKMQLQELVDKGYIRPSVSPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 595

Query: 61  YPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKESDILKNS 103
           YPLPRIDDLFDQL GA++FSKIDLRSGYHQLKV+ESDI K +
Sbjct: 596 YPLPRIDDLFDQLRGAALFSKIDLRSGYHQLKVRESDIAKTA 637

BLAST of Cmc01g0023381 vs. ExPASy TrEMBL
Match: A0A5A7U2V7 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00630 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.8e-44
Identity = 88/102 (86.27%), Postives = 94/102 (92.16%), Query Frame = 0

Query: 1   MAPMELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 60
           MAP EL+ELK QLQELVD G+IRPS SPWGAPVLFVKKKDGTLRLCIDYRQLNKVTI NK
Sbjct: 627 MAPSELKELKMQLQELVDKGYIRPSVSPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIRNK 686

Query: 61  YPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKESDILKNS 103
           YPLPRIDDLFDQL GA++FSKIDLRSGYHQLKV+ESDI K +
Sbjct: 687 YPLPRIDDLFDQLRGAALFSKIDLRSGYHQLKVRESDIAKTA 728

BLAST of Cmc01g0023381 vs. ExPASy TrEMBL
Match: A0A5D3BS67 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold509G00050 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.8e-44
Identity = 88/102 (86.27%), Postives = 94/102 (92.16%), Query Frame = 0

Query: 1   MAPMELRELKSQLQELVDTGFIRPSASPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIHNK 60
           MAP EL+ELK QLQELVD G+IRPS SPWGAPVLFVKKKDGTLRLCIDYRQLNKVTI NK
Sbjct: 665 MAPSELKELKMQLQELVDKGYIRPSVSPWGAPVLFVKKKDGTLRLCIDYRQLNKVTIRNK 724

Query: 61  YPLPRIDDLFDQLHGASVFSKIDLRSGYHQLKVKESDILKNS 103
           YPLPRIDDLFDQL GA++FSKIDLRSGYHQLKV+ESDI K +
Sbjct: 725 YPLPRIDDLFDQLRGAALFSKIDLRSGYHQLKVRESDIAKTA 766

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK18080.11.3e-4895.10hypothetical protein E5676_scaffold306G004160 [Cucumis melo var. makuwa][more]
KAA0042141.11.3e-4895.10hypothetical protein E6C27_scaffold67G006300 [Cucumis melo var. makuwa][more]
KAA0061889.13.4e-4486.27DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
TYK27290.11.0e-4386.27DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
KAA0050527.11.0e-4386.27DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q993157.7e-2348.96Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20... [more]
Q7LHG57.7e-2348.96Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20... [more]
P103942.6e-1843.12Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaste... [more]
Q9UR079.8e-1838.14Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT419.8e-1838.14Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
Match NameE-valueIdentityDescription
A0A5D3D2Y26.5e-4995.10Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7TKQ06.5e-4995.10Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5A7V8731.7e-4486.27DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A5A7U2V74.8e-4486.27Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37... [more]
A0A5D3BS674.8e-4486.27Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold50... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR043128Reverse transcriptase/Diguanylate cyclase domainGENE3D3.30.70.270coord: 61..88
e-value: 1.0E-42
score: 147.9
NoneNo IPR availableGENE3D3.10.10.10HIV Type 1 Reverse Transcriptase, subunit A, domain 1coord: 1..100
e-value: 1.0E-42
score: 147.9
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 12..101
NoneNo IPR availablePANTHERPTHR24559:SF319SUBFAMILY NOT NAMEDcoord: 12..101
NoneNo IPR availableCDDcd01647RT_LTRcoord: 20..100
e-value: 7.61823E-43
score: 136.186
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 2..102

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc01g0023381.1Cmc01g0023381.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity
molecular_function GO:0008270 zinc ion binding