CmaCh20G011420 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G011420
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionNASP-related protein sim3
LocationCma_Chr20: 9040979 .. 9046168 (+)
RNA-Seq ExpressionCmaCh20G011420
SyntenyCmaCh20G011420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCGCCCAAAAAGGATTTTAAAGCTTGAAGTGCGGACTCCTCTGCTTAAACCTCCATTTTCTCCAAGCTTCGAAACTATAGCCATGGCCGACGAAGATCCACCATCGGAGCTCTCAGTGACGTTGCAGAAACCGAAACTCGACGAAATCCTGAACGTCAGTAAGGCCACCACTGAGTCCGATGTTCTGGGAGGCCTCGAGTCGTCTTGCAATTGTTCCAATGAAACGAAGCCCGGTGCTGAATCCACTGCTCAAACCTCGGATGGAAGTGGTGAAAAATCGTTGGAGCTGGCGGAAGAGTTGCTGGAGAAGGGTTCCAAGGCTATTAAGGACAACGATTTCGTTGAGGCTGTCGATTGCTTCAGCCGTGCCCTTGAGATTAGGTTAGGGTTTGGTTATTTTTCACTTTCTCTTCAGTTTCTTTTTGATTATGAATTGAATTCTGGAACGAAGATGAAAGTCGAGCTTCTATTACCCTTGAAGTCTTAAAGTTGATTTGTTACAATTGCTTCACTCTGTTAGATCACATGTTTCCACTTATCATTTTGTCGGTTTTAAGATTTTCATCTTGGGTAAATTTTAATTCCATTTTCTTTAGCGGTGGGGGAGCTTTTCGTTGCTCCAGATTCATTTTCGGAACTTTATCTTTCTTTTATTGAAGATCAACCGCTCGTCAGGATCACCGAACAGTCTTGGTAGATATGAATTGAGTTTTAACGAGGTTTTGTAATGTTGTTAGGTTCCAACTTTATTTAACTTTTGCATATAAGAACTTGTTGCTCATTTTTTTTTCTTGTCTATATCATTGGATACCTGTTGCGAACATGTCTCTTTTACGATGTGAAAAGTGTATTCTTAAATCAAGCATTTGCATGCGTTTAGTTTCATCCTTTGATTTGTTTTTTCTAGCAATAATCTTGAAAATATTTCACTGCAGAGCTGCGCGATATGGTGAACTGGCTCCGGAATGTGTTAAATTGTACTACAAATATGGATGTGCTTTATTGTACAAAGCCCAGGAGGAGGCAGATCCACTGGGAGCTGTCCCAAAGAAAGAAGGTGAACCCCATCAACAATCTTACAAAGATGAATCTGTTAAGAGTGCTGAAAATGGCGAGTCATCAAAAGCTTCTGTTTCCAGCAATGCTGAACTGGTGGATGGAGTTGCAGATGATGGTTGGTATCTTCCTCATGATGTCTAACTTATCTAGTTCCTAACATACATTTCCTTTCTCTTTTCCTTGGATATTTTCTATTTTACATTATTTAAAATCTACATGAAACATGGCATGTTGCAGCGTAATGAATTCCCCTTTAATATTTGACTAACTTGAAGAGGATTCAACCTTGATTTACTTATTCTCTCGCCTTTCCCTTCCTTAAAGATAGAGAGGATCTTATATTATATACTTGAGCATTGAGAGCAACATGCTTCTGGTTTTATTTTATCTTTGTTTCATTGATGTATTTCTTGGTAACCTAAGCAATGAATTTGGTGCTACAGAAGCATACTGAGCATGAATAAATTGCTGCTGGTTTTATGGTGTCTAAGTTTCATTGAAGTAGTTCAACTGAATACGAAGAGATCAATTACTTTTTTTCAATGTATTGTTTTATTTCGGTATTGAGATGTTACAAGCTCTATAGCTTTTTTTTTTTAATTTAATTTTATTTTTTTATGGACATCAGAATGTGATGATTTTCATTGTTTCGTGAAAAGCAGGATTTTTTATATATTGCTAAGTTAGAATGCATACACGTTAATCATTGATGACTATCATCATTTCCTGTTTAGAACACACCTACATGTTCGCTAGGCTTCCAGCTCTAATTGGATAGGTATCTTGCCTTCTGTGGAAGTCATTTGACTTGAGTCTAGGTGACTGACATACCTTGGCGTGATGACTTATCGCAGTGGACATTCAAGTACCCTTCCTTATGTCTTGCATATAAGTGCCTTTACGGAAGCTTTGTCTTCCACTTTAGTTTCTTGTTTCCTCCTTTCACTTTGTCTCTATCGTTTCCTTTTATTCTCTCTTTTCAAAGTGCTTGCAGCAACATTTTATGAATTTTTATGTCCTGTAATATTTCAATATTTAGAATGAATTACTTTTCCATGTGATATCATGCACCTCACCTCATCTTGGCTTGCATCTTCTTTTTTTGCATCTCCAGTGTGGCTCTGTGCATCTTTTTTCATTTTTCAATCCACTTAGTGCTTTAGAAAATGCCGATCACTTTATACATTATTTTAGAATTTGATGAATTATAAATTTCTGAAGTTTTATTGTTTGAGTTGCAAATGGGAATATTAACTACAGGTCTTTAGGGATCTTTACAACTTGTGTTCAACAAATTATTGATGCCTTATTGCTTTACTTCTGGATGTGTCCCCACTGTCACACTCGTCAATATTTACCTTCTCATGTTGTACATATTATCTTAAGAAAAAAAAAAAAAAAAAAAAATTAGCTGCAGTGATACAGCCACCGTCCAGTTTTATTACAAAAAAAAGTTAATGCTATCATCATCCTATGTAGTCGTTTCAGAGCTTTGGGGCCCTAAATCCTAAGAAATAACTTGAATAAATTTTTGGTGATTAAGACGAGACATCTGCCTTGGAATTCTTTGCTAACTTGCTGATGTCTATCTGGCCATTTAATCTTAACTTCCCTTATGTGGTCAAAATACTTTTTTAAGAAAAGTTTTTGCATTGAATCATCGTGTTTGCAACAACAGATTTTTATTGTATTCAATTTCAATAATTTTATAAATTTCTATCTATGTAATCAAGCACTTTGAATAGAACAATTTTCTTGGGGCTTGTAGTTTACTGCTTTACTCATCATTTGAATGAATTTGCTGTTTCTTGTTTTTAAAAGTACAATTATGTAGATTTGATGGTACCTACTGATGCATCAAAAAGAAGTTGTATCTTATCTTCCTTTATAGGAGTTTTGTTTGATGTATAACGTACATGGAATCTCAACTTCTATCATGTATCATTTTTACATGCATTAACTGTGAATCACTTAAATTATGAACAGTTACTAGGATGACAGTCGTTTTCAACTGTGAAGAGGCATGTATAAATGCTCTTCAATTTTGAATGATGGACTATCATGCTTAACATTTTGTTCCGCTTGATTTTGACTGTGTTATTTTATTGCTGGTCTTCGTTTTAAGATTATCCCCATCTTTTATCTATAAAAAATGTACATTTTTAAAAAAGTTATCTCATTAGGAGATTTTTGACCTTCCATTTTCCCTTTTTTTTTTTTTTCTTTGAGACAGTTTCCAGTAAGAAAGATCAGGATGAAGAAGAGGGTGATGATAGTGGTACTGAGGACTTAGCAGATGCAGATGAAGACGAATCTGACCTTGATTTAGCATGGAAAATGCTAGACGTTGCCAGAGCAATAGTTGAAAAAGACTCGGGTGATACGATGGAGAAAGTAGACATATTGTCAGCCTTGGCAGAAGTTGCTCTGGAAAGAGGTATACATGCATTTTATTTGCTCTGTTGTGATACACATTCAGTTGGTTTGTGTTTCTTTGATATTATTCCCCTTTCTTTCTCGGGTTAGAGGGTTATAGGTTATCCTTTAATTTTAATTTTAAAGTTCATTTTTTTTCTCTCTCCTTCATGTAGAGGACATTGAAACTTCCCTCAGTGACTACCAGAAAGCATTATCAATTTTAGAAAGACTTGTCGAACCTGACAATCGACAGCTTGCTGAACTGTATCCTTCTTTCAGATTCTAAATCTCATATAAGTACGTAAATAGAATATAATATATACGTAAAAATCTGTTTTCAAAATATTCTAACCGAGAATTCAAGATAAAAAGAAGAAAAAACATCCTGTTTTCAAGGTAGCCGACTTCAATTCTGTTTATAATAGTAGCTTATAAATTCTGGGAGCTTGTTAGTCTATGTTTCTACCTCGTTTATAATAGTATCTTATAAATTCTAGGAGCTTGATGATAAAAGTTTAGTTTTTATTTTAATTATATATTGCTTTTTCTTAAGAAAAAGAAAACCGTATGAATTGCTATTATTGACATTTGAACCACATCACTTCCTTTCAAGTGAGGAAAGTGTCTTTTGCAACCTTAAACTGAAACTTCCAGAAACTTCCGTGTATGCTTGTGCCTGGAGTTTGGTTCTCAGCCGCAGGAAGCCATTTCATTTTGCCAGAAGGCAATATCAATTTGCAAGTCACGTGTGATGCGGCTTACTGATGAAGTAAAGGGTATCATTGTACCGACCACAGCTTCTTCTACTTCTGGGTCAGAACCAGAGGTCCCACTATCGTCCAATGACTCCCAGTCTGACCATGCCAATGCTGCAACAGAGAAACAATCTGAGATTGAAATTCTATCTGGGCTTTTGGTCGAGCTAGAAAAGAAGGCGAGTTTAGATTATAACTTCTGCAAGTAACTGTGACAGAGAAATGGAAAAATCTAACCACTGTTTTGTGTTTGTGCAGCTTGAAGATCTGCAACAGCTGGCCTCAAACCCAAAGTCAATCCTCTCGGAGATCCTCGGGATAGGAGCGGCGAAGGCAAAAGTCGAAAAGATCGCTCCTCCACTTCCAGCAGTGTTGAACTCCTCACAGATGGGTTCAGCTAACAGCAATGGAGGATTTGACTCTCCAACGGTCTCAACTGCACACACGAACGGCGCGGCTGGAGTGACGCACCTTGGCGTCGTTGGAAGGGGAGTCAAACGAGTATCAACAAATTCAGAGTCTGCTGACTCCTCCCACCCAACGAAGAAACGGGCAACAGATTCATCAACACAAGATAAAGGTGATGGCAGTTCCGCCTGAAGAGGTTGTGTTCTCGAACTCATTTTGAAATTAGATATATCACATAAGTGCATAGCAGTAAAAGTAGATTCAGAATCATCCTGCCTGTGGCTGTTATCATTTCATGTATGTTTGTACTATGTTCGACTAATATCTTACTCTGAATGATGTTTTTCCAGACCTCCTAAATCCAAGTTCTAGTAATCATCCAGCAGTTTTATGTTTGTCACTTCACTCTTTGGGGCAATTCATGATTATACTGATTTATTTTTGTAATTTGTAGAAGTCAACCTAATAATTTGTAATTTGTGTCATATTGTTACAATATTATATTGATACCCGTGGATATCTATTTAGACATGACTCAACCAAGAAAT

mRNA sequence

TTCCGCCCAAAAAGGATTTTAAAGCTTGAAGTGCGGACTCCTCTGCTTAAACCTCCATTTTCTCCAAGCTTCGAAACTATAGCCATGGCCGACGAAGATCCACCATCGGAGCTCTCAGTGACGTTGCAGAAACCGAAACTCGACGAAATCCTGAACGTCAGTAAGGCCACCACTGAGTCCGATGTTCTGGGAGGCCTCGAGTCGTCTTGCAATTGTTCCAATGAAACGAAGCCCGGTGCTGAATCCACTGCTCAAACCTCGGATGGAAGTGGTGAAAAATCGTTGGAGCTGGCGGAAGAGTTGCTGGAGAAGGGTTCCAAGGCTATTAAGGACAACGATTTCGTTGAGGCTGTCGATTGCTTCAGCCGTGCCCTTGAGATTAGAGCTGCGCGATATGGTGAACTGGCTCCGGAATGTGTTAAATTGTACTACAAATATGGATGTGCTTTATTGTACAAAGCCCAGGAGGAGGCAGATCCACTGGGAGCTGTCCCAAAGAAAGAAGGTGAACCCCATCAACAATCTTACAAAGATGAATCTGTTAAGAGTGCTGAAAATGGCGAGTCATCAAAAGCTTCTGTTTCCAGCAATGCTGAACTGGTGGATGGAGTTGCAGATGATGTTTCCAGTAAGAAAGATCAGGATGAAGAAGAGGGTGATGATAGTGGTACTGAGGACTTAGCAGATGCAGATGAAGACGAATCTGACCTTGATTTAGCATGGAAAATGCTAGACGTTGCCAGAGCAATAGTTGAAAAAGACTCGGGTGATACGATGGAGAAAGTAGACATATTGTCAGCCTTGGCAGAAGTTGCTCTGGAAAGAGAGGACATTGAAACTTCCCTCAGTGACTACCAGAAAGCATTATCAATTTTAGAAAGACTTGTCGAACCTGACAATCGACAGCTTGCTGAACTAAACTTCCGTGTATGCTTGTGCCTGGAGTTTGGTTCTCAGCCGCAGGAAGCCATTTCATTTTGCCAGAAGGCAATATCAATTTGCAAGTCACGTGTGATGCGGCTTACTGATGAAGTAAAGGGTATCATTGTACCGACCACAGCTTCTTCTACTTCTGGGTCAGAACCAGAGGTCCCACTATCGTCCAATGACTCCCAGTCTGACCATGCCAATGCTGCAACAGAGAAACAATCTGAGATTGAAATTCTATCTGGGCTTTTGGTCGAGCTAGAAAAGAAGCTTGAAGATCTGCAACAGCTGGCCTCAAACCCAAAGTCAATCCTCTCGGAGATCCTCGGGATAGGAGCGGCGAAGGCAAAAGTCGAAAAGATCGCTCCTCCACTTCCAGCAGTGTTGAACTCCTCACAGATGGGTTCAGCTAACAGCAATGGAGGATTTGACTCTCCAACGGTCTCAACTGCACACACGAACGGCGCGGCTGGAGTGACGCACCTTGGCGTCGTTGGAAGGGGAGTCAAACGAGTATCAACAAATTCAGAGTCTGCTGACTCCTCCCACCCAACGAAGAAACGGGCAACAGATTCATCAACACAAGATAAAGGTGATGGCAGTTCCGCCTGAAGAGGTTGTGTTCTCGAACTCATTTTGAAATTAGATATATCACATAAGTGCATAGCAGTAAAAGTAGATTCAGAATCATCCTGCCTGTGGCTGTTATCATTTCATGTATGTTTGTACTATGTTCGACTAATATCTTACTCTGAATGATGTTTTTCCAGACCTCCTAAATCCAAGTTCTAGTAATCATCCAGCAGTTTTATGTTTGTCACTTCACTCTTTGGGGCAATTCATGATTATACTGATTTATTTTTGTAATTTGTAGAAGTCAACCTAATAATTTGTAATTTGTGTCATATTGTTACAATATTATATTGATACCCGTGGATATCTATTTAGACATGACTCAACCAAGAAAT

Coding sequence (CDS)

ATGGCCGACGAAGATCCACCATCGGAGCTCTCAGTGACGTTGCAGAAACCGAAACTCGACGAAATCCTGAACGTCAGTAAGGCCACCACTGAGTCCGATGTTCTGGGAGGCCTCGAGTCGTCTTGCAATTGTTCCAATGAAACGAAGCCCGGTGCTGAATCCACTGCTCAAACCTCGGATGGAAGTGGTGAAAAATCGTTGGAGCTGGCGGAAGAGTTGCTGGAGAAGGGTTCCAAGGCTATTAAGGACAACGATTTCGTTGAGGCTGTCGATTGCTTCAGCCGTGCCCTTGAGATTAGAGCTGCGCGATATGGTGAACTGGCTCCGGAATGTGTTAAATTGTACTACAAATATGGATGTGCTTTATTGTACAAAGCCCAGGAGGAGGCAGATCCACTGGGAGCTGTCCCAAAGAAAGAAGGTGAACCCCATCAACAATCTTACAAAGATGAATCTGTTAAGAGTGCTGAAAATGGCGAGTCATCAAAAGCTTCTGTTTCCAGCAATGCTGAACTGGTGGATGGAGTTGCAGATGATGTTTCCAGTAAGAAAGATCAGGATGAAGAAGAGGGTGATGATAGTGGTACTGAGGACTTAGCAGATGCAGATGAAGACGAATCTGACCTTGATTTAGCATGGAAAATGCTAGACGTTGCCAGAGCAATAGTTGAAAAAGACTCGGGTGATACGATGGAGAAAGTAGACATATTGTCAGCCTTGGCAGAAGTTGCTCTGGAAAGAGAGGACATTGAAACTTCCCTCAGTGACTACCAGAAAGCATTATCAATTTTAGAAAGACTTGTCGAACCTGACAATCGACAGCTTGCTGAACTAAACTTCCGTGTATGCTTGTGCCTGGAGTTTGGTTCTCAGCCGCAGGAAGCCATTTCATTTTGCCAGAAGGCAATATCAATTTGCAAGTCACGTGTGATGCGGCTTACTGATGAAGTAAAGGGTATCATTGTACCGACCACAGCTTCTTCTACTTCTGGGTCAGAACCAGAGGTCCCACTATCGTCCAATGACTCCCAGTCTGACCATGCCAATGCTGCAACAGAGAAACAATCTGAGATTGAAATTCTATCTGGGCTTTTGGTCGAGCTAGAAAAGAAGCTTGAAGATCTGCAACAGCTGGCCTCAAACCCAAAGTCAATCCTCTCGGAGATCCTCGGGATAGGAGCGGCGAAGGCAAAAGTCGAAAAGATCGCTCCTCCACTTCCAGCAGTGTTGAACTCCTCACAGATGGGTTCAGCTAACAGCAATGGAGGATTTGACTCTCCAACGGTCTCAACTGCACACACGAACGGCGCGGCTGGAGTGACGCACCTTGGCGTCGTTGGAAGGGGAGTCAAACGAGTATCAACAAATTCAGAGTCTGCTGACTCCTCCCACCCAACGAAGAAACGGGCAACAGATTCATCAACACAAGATAAAGGTGATGGCAGTTCCGCCTGA

Protein sequence

MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSDGSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGCALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDVSSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSALAEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQKAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEILSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANSNGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGDGSSA
Homology
BLAST of CmaCh20G011420 vs. ExPASy Swiss-Prot
Match: Q17886 (Protein NASP homolog 1 OS=Caenorhabditis elegans OX=6239 GN=nasp-1 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 6.9e-07
Identity = 87/404 (21.53%), Postives = 159/404 (39.36%), Query Frame = 0

Query: 43  NCSNETKPGAESTAQTSDGSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAA 102
           + S ++      T    +   +K   LA ELL  G +A+K ND  +A D  S A E+ + 
Sbjct: 16  DASGDSDEKGNGTTTEEETVEQKEKRLA-ELLAAGRRALKVNDIDKASDSLSEATELSSE 75

Query: 103 RYGELAPECVKLYYKYGCALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESS 162
            YGE         Y YG A L  A+EE+  L    +KE    +Q+   +     ENGE+ 
Sbjct: 76  IYGENHENTFDSLYYYGMATLELAKEESQLLKGPGEKESGDEEQAGNSDDKTDEENGETE 135

Query: 163 KASVSSNAELVDGVADDVSSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVAR-- 222
           K                         E+G++SG E+    D+D+  + L+W++L+ AR  
Sbjct: 136 K-------------------------EDGEESGEEE----DDDDDTMKLSWEILETARCI 195

Query: 223 ------AIVEKDSGDT------MEKVDILSALAEVALEREDIETSLSDYQKALSILERLV 282
                 A+  + SG +      ++  D+L  L E  +       +  D  +AL+I   ++
Sbjct: 196 AAAKIEALEAEQSGISAIEEWNLKLADVLVLLGEHGISDGKYTQAFEDLDRALNIQRNVL 255

Query: 283 EPDNRQLAELNFRVCLCLEFGSQPQEAISFCQKAISICKSRVMRLTDEVKGIIVPTTASS 342
            P +R++A+    +       +   E + +  K   +  +R   L  E+           
Sbjct: 256 PPSSRKIAQTYILIGNACASDANYDETVQYFGKTKDVLIARQTELKHEL----------- 315

Query: 343 TSGSEPEVPLSSNDSQSDHANAATEKQSEIEILSGLLVELEKKLEDLQQLASNPKSILSE 402
                 E  +   + +S+  N   E +   E++ G+   +   +    Q+    K+I ++
Sbjct: 316 ------ERGVDDKEKKSEFENELKELE---EMMPGVEEMIADAVHSAAQVEETKKAIKAQ 369

Query: 403 ILGIGAAKAKVEKIAPPLPAVLNSSQMGSANSNGGFDSPTVSTA 433
             G     AK+ + A       + S +    +    D+PT + A
Sbjct: 376 FEGFTQVLAKLPQEAGDQKEANDISSLVRRPAKRAVDAPTDNQA 369

BLAST of CmaCh20G011420 vs. ExPASy Swiss-Prot
Match: Q2T9P4 (Nuclear autoantigenic sperm protein OS=Bos taurus OX=9913 GN=NASP PE=2 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 1.5e-06
Identity = 69/260 (26.54%), Postives = 130/260 (50.00%), Query Frame = 0

Query: 128 EEADPLG-AVPKKEGEPHQQSYKDESVKSAENGES-SKASVSSNAELVDGVADDVSSKKD 187
           EE++  G  V  +  +   +   ++ VK A N E+  K       E  +G +++   + D
Sbjct: 419 EESEAAGDGVETEVAQGATEQSPEDKVKIAANEEAQDKEEQMKEGEETEG-SEEEDKEND 478

Query: 188 QDEEEGDDSGTED--LADADEDE-SDLDLAWKMLDVARAIVEKDSGDTMEKVDILSA--- 247
           + EE  +DS  E+  L + +E+E  +L+LAW MLD+A+ I ++   DT E   + +A   
Sbjct: 479 KAEETLNDSALENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQ--DTKE-AQLYAAQAH 538

Query: 248 --LAEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAIS 307
             L EV++E E+   ++ ++Q  L++ E+ +E  +R LAE ++++ L   + SQ  EA++
Sbjct: 539 LKLGEVSVESENYLQAVEEFQACLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVA 598

Query: 308 FCQKAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSE 367
              K+I + + R+  L +++K                           +   + TE + E
Sbjct: 599 QFSKSIEVIEKRMAVLNEQMK---------------------------EAEGSPTEYEKE 647

Query: 368 IEILSGLLVELEKKLEDLQQ 378
           IE L  LL E+ +K+ED ++
Sbjct: 659 IEELKELLPEIREKIEDAKE 647

BLAST of CmaCh20G011420 vs. ExPASy Swiss-Prot
Match: Q66HD3 (Nuclear autoantigenic sperm protein OS=Rattus norvegicus OX=10116 GN=Nasp PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 9.9e-06
Identity = 100/438 (22.83%), Postives = 181/438 (41.32%), Query Frame = 0

Query: 34  VLGGLESSCNCSNETKPGAESTAQTSDGSGEKSLELAEELLEKGSKAIKDNDFVEAVDCF 93
           V+G   +     +E +  AE  ++T DG+  +  E+  EL+ +  +A+     VE  +  
Sbjct: 369 VVGQSSAGDQTPSEPQNSAERLSETKDGASVE--EVKAELVPEQEEAMPP---VEESEAA 428

Query: 94  SRALEIRAA-RYGELAPECVKLYYKYGCALLYKAQEEADPLGAVPKKEGEPHQQSYKDES 153
              +E + A R  E APE      K+  A   + QE                    +DE 
Sbjct: 429 GDGVETKVAQRATEKAPE-----DKFKIAANEETQE--------------------RDEQ 488

Query: 154 VKSAENGESSKASVSSNAELVDGVADDVSSKKDQDEEEGDDSGTEDLADADEDESDLDLA 213
           +K  E  E S+     N +  +   + V  KK   E E            +E+  +L+LA
Sbjct: 489 MKEGEETEGSEEEDKENDKAEETTNESVLEKKTLQENE------------EEEIGNLELA 548

Query: 214 WKMLDVARAIVEKDSGDTMEKVDILSA-----LAEVALEREDIETSLSDYQKALSILERL 273
           W MLD+A+ I ++      ++  + +A     L EV++E E+   ++ ++Q  LS+ E+ 
Sbjct: 549 WDMLDLAKIIFKRQE---TKEAQLYAAQAHLKLGEVSVESENYIQAVEEFQACLSLQEQY 608

Query: 274 VEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQKAISICKSRVMRLTDEVKGIIVPTTAS 333
           +E  +R LAE ++++ L   + SQ  EA++   K+I + + R+  L +++K         
Sbjct: 609 LEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFGKSIDVIEKRMAVLLEQMK--------- 668

Query: 334 STSGSEPEVPLSSNDSQSDHANAATEKQSEIEILSGLLVELEKKLEDLQQLASNPKSILS 393
                             +   + TE + EIE L  LL E+ +K+ED ++         S
Sbjct: 669 ------------------EAEGSFTEYEKEIEELKELLPEIREKIEDAKE---------S 725

Query: 394 EILGIGAAKAKVEKIAPPLPAVLNSSQMGSANSNGGFDSPTVSTAHTNGAAGVTHLGVVG 453
           +  G  A  A    +     +    S  G++ S      PT   + +N    ++HL    
Sbjct: 729 QRSGNVAELALKATLVESSTSGFTPSGAGASVSMIASRKPTDGASSSNCVTDISHLVRKK 725

Query: 454 RGVKRVSTNSESADSSHP 466
           R  +  S   + A  + P
Sbjct: 789 RKPEEESPRKDDAKKAKP 725

BLAST of CmaCh20G011420 vs. ExPASy Swiss-Prot
Match: P06180 (Histone-binding protein N1/N2 OS=Xenopus laevis OX=8355 PE=1 SV=3)

HSP 1 Score: 51.6 bits (122), Expect = 2.9e-05
Identity = 81/354 (22.88%), Postives = 151/354 (42.66%), Query Frame = 0

Query: 151 ESVKSAENGESSKASVSSNAELVDGVADDVSSKKD----QDEEEGDDS-----------G 210
           +S + AE    S+AS S  +  +    ++ +   D    ++EEEG+DS           G
Sbjct: 257 DSTEVAEEKMDSEASESKESTSIPPTENEANKPDDPEKMEEEEEGEDSEENEDGTEENEG 316

Query: 211 TEDLADADEDESDLDLAWKMLDVARAIVEKDSGD--TMEKVDILSALAEVALEREDIETS 270
           TE+    +ED  +L LAW+MLD+ + I ++       ++       L EV +E E+   +
Sbjct: 317 TEEKETEEEDVGNLQLAWEMLDLCKTIFKRQQSKEAQLKAAQAHQKLGEVCIESENYSQA 376

Query: 271 LSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQKAISICKSRVMRL 330
           + D+   L+I +  +E  +R LAE ++ + L  ++ S+ +EAIS   ++I + + R+  L
Sbjct: 377 VEDFLACLNIQKEHLEEHDRLLAETHYHLGLAYQYSSKHEEAISHFTQSIGVIEKRMDVL 436

Query: 331 TDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEILSGLLVELEKKLE 390
           T +++  +                               E + E++ L  LL ++++K+E
Sbjct: 437 TKQLEASV--------------------------GELVDEVKKEMDELKDLLPDIKEKIE 496

Query: 391 DLQQLASN---PKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANSNGGFDSPTVS 450
           D ++   N    +  L E L  G++                S + GS +S+   +    S
Sbjct: 497 DSKEAQKNATVTEKALKETLVGGSSGF--------------SKENGSTSSSSAVEKSGDS 556

Query: 451 TAH-TNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGDGSS 484
           T   TN  + ++HL       K+  T  ES       KK   +      G+G +
Sbjct: 557 TVPVTNCVSDISHL-----VRKKRKTEEESPLKDKDAKKSKQEPVANGAGNGDA 565

BLAST of CmaCh20G011420 vs. ExPASy Swiss-Prot
Match: Q9USQ4 (NASP-related protein sim3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sim3 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 4.2e-04
Identity = 93/392 (23.72%), Postives = 180/392 (45.92%), Query Frame = 0

Query: 38  LESSCNCSNETKPGAESTAQTSDGSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRAL 97
           + S       +K  + + A T + S   S  + E+L+ +G+ A    ++ EAVD + +AL
Sbjct: 1   MSSDTKTLENSKGNSATDADTKNPSSSDSRAI-EQLVTQGNMAYAQKNYEEAVDKYGQAL 60

Query: 98  EIRAARYGELAPECVKLYYKYGCALLYKAQEEADPLG-AVPKKEGEPHQQSYKDESVKSA 157
               + +G  + E   + + YG +L   A E +  LG A+  KE          ++ +S 
Sbjct: 61  MQSESIHGSESLENRNVLWLYGKSLFQIAIENSQVLGNALGAKES-------VSQATESF 120

Query: 158 ENGESSKASVSSNAELVDG-VADDVSSKKDQDEEEGDDSGTEDLADA-DEDESDLDLAWK 217
           E  E+  +   S  ++ +    ++ +S     E+E ++  T + + A +EDE D ++AW+
Sbjct: 121 EEPEAIGSFTFSGQKIENKYTVNEENSSIAHPEKESEEKETNEASPASEEDEDDFNVAWE 180

Query: 218 MLDVARAIVEK------DSGD-TMEKVDILSALAEVALEREDIETSLSDYQKALSILERL 277
           +LD+ R +  K      DS D  +   DI   L E++LE E+   +  D + AL   E++
Sbjct: 181 VLDLTRVMQSKAVDAYPDSKDEKIRLADIYDLLGELSLEIENFSQASQDLKTALEWKEKV 240

Query: 278 VE-PDNRQLAELNFRVCLCLEFGSQPQEAISFCQKAISICKSRVMRLTDEVKGIIVPTTA 337
               +N  L+E ++++ L LEF + P++  +         KSR     ++   I+     
Sbjct: 241 YNVSNNTLLSEAHYKLALALEF-TNPEDPSN---------KSRACEHVEKAAEILKNVL- 300

Query: 338 SSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEILSGLLVELEKKLEDLQQLA-SNPKSI 397
                +E E  ++    +       +   S++E L  +L ELE+K  DL+  A S  +++
Sbjct: 301 -----NERENEVTDKKGKGKQKAEESTLTSDLENLREMLSELEQKTLDLKHGAPSLEEAV 360

Query: 398 LSEILGIGAAKAKVEKIAPPL-PAVLNSSQMG 417
           +S++            +A  +  AV N++ +G
Sbjct: 361 MSKMHESSLLSKDSSSLAQAVAEAVKNANDLG 368

BLAST of CmaCh20G011420 vs. ExPASy TrEMBL
Match: A0A6J1I412 (protein HGV2 OS=Cucurbita maxima OX=3661 GN=LOC111470382 PE=4 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 4.3e-254
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD 60
           MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD
Sbjct: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD 60

Query: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120
           GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC
Sbjct: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120

Query: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV 180
           ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV
Sbjct: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV 180

Query: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240
           SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL
Sbjct: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240

Query: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300
           AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ
Sbjct: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300

Query: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEI 360
           KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEI
Sbjct: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEI 360

Query: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 420
           LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS
Sbjct: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 420

Query: 421 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD 480
           NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD
Sbjct: 421 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD 480

Query: 481 GSSA 485
           GSSA
Sbjct: 481 GSSA 484

BLAST of CmaCh20G011420 vs. ExPASy TrEMBL
Match: A0A6J1EJQ1 (protein HGV2 OS=Cucurbita moschata OX=3662 GN=LOC111434946 PE=4 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 2.9e-250
Identity = 476/484 (98.35%), Postives = 482/484 (99.59%), Query Frame = 0

Query: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD 60
           MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGL+SSCNCSNETKP AESTAQTSD
Sbjct: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLDSSCNCSNETKPAAESTAQTSD 60

Query: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120
           GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC
Sbjct: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120

Query: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV 180
           ALLYKAQEEADPLGAVPKKEGEP+QQSYKDESVK+ ENGESSKASVSSNAELVDGVADDV
Sbjct: 121 ALLYKAQEEADPLGAVPKKEGEPYQQSYKDESVKNVENGESSKASVSSNAELVDGVADDV 180

Query: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240
           SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL
Sbjct: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240

Query: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300
           AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ
Sbjct: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300

Query: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEI 360
           KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPE+PLSSNDSQ+D+ANAATEKQSEIEI
Sbjct: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEIPLSSNDSQTDNANAATEKQSEIEI 360

Query: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 420
           LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS
Sbjct: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 420

Query: 421 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD 480
           NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD
Sbjct: 421 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD 480

Query: 481 GSSA 485
           GSSA
Sbjct: 481 GSSA 484

BLAST of CmaCh20G011420 vs. ExPASy TrEMBL
Match: A0A1S3C6L8 (NASP-related protein sim3 OS=Cucumis melo OX=3656 GN=LOC103497438 PE=4 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 5.5e-209
Identity = 419/487 (86.04%), Postives = 437/487 (89.73%), Query Frame = 0

Query: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD 60
           MADEDPPSE+SVT+ KPKLDE LNVS+ TTES   GGL+SSCN  NE KP  E TAQTSD
Sbjct: 1   MADEDPPSEVSVTVDKPKLDETLNVSEVTTESIAQGGLDSSCNSPNEKKPITEPTAQTSD 60

Query: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120
           GSGEKSLELAEELLEKGSKA+KDNDF EAVDCFSRALEIRAA YGELA ECVKLYYKYGC
Sbjct: 61  GSGEKSLELAEELLEKGSKAMKDNDFNEAVDCFSRALEIRAAHYGELASECVKLYYKYGC 120

Query: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV 180
           ALLYKAQEEADPLGAVPKKEG    QS K ES KSA NGESSKASVSSNAE+VDGV DDV
Sbjct: 121 ALLYKAQEEADPLGAVPKKEG----QSDKAESAKSAVNGESSKASVSSNAEVVDGVTDDV 180

Query: 181 S---SKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDIL 240
           S   SKKDQDEEE DDS  EDLADADEDESDLDLAWKMLDVARAIVEKDS DTMEKVDIL
Sbjct: 181 SETVSKKDQDEEETDDSDAEDLADADEDESDLDLAWKMLDVARAIVEKDSADTMEKVDIL 240

Query: 241 SALAEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAIS 300
           SALAEVALEREDI TSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAIS
Sbjct: 241 SALAEVALEREDIGTSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAIS 300

Query: 301 FCQKAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSE 360
           +CQKAISICKSRV+RLTDEVK +IVPTTASSTSGSEPE+PLSSN SQ+D+ NAATEKQSE
Sbjct: 301 YCQKAISICKSRVVRLTDEVKSVIVPTTASSTSGSEPEIPLSSNGSQTDNENAATEKQSE 360

Query: 361 IEILSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGS 420
           IE LSGLLVELEKKLEDLQQLASNP SILSEILGIG+AK  +EKI PP+P+V NSSQMGS
Sbjct: 361 IETLSGLLVELEKKLEDLQQLASNPMSILSEILGIGSAKPNIEKITPPVPSVFNSSQMGS 420

Query: 421 ANSNGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQD 480
           ANSNGGFDSPTVSTAHTN   GVTHLGVVGRGVKRVSTNSES D S+PTKK A D S+QD
Sbjct: 421 ANSNGGFDSPTVSTAHTN---GVTHLGVVGRGVKRVSTNSESND-SNPTKKLAKDLSSQD 479

Query: 481 KGDGSSA 485
           KGD SSA
Sbjct: 481 KGDSSSA 479

BLAST of CmaCh20G011420 vs. ExPASy TrEMBL
Match: A0A6J1E053 (NASP-related protein sim3 OS=Momordica charantia OX=3673 GN=LOC111025157 PE=4 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 1.6e-208
Identity = 414/485 (85.36%), Postives = 440/485 (90.72%), Query Frame = 0

Query: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD 60
           MADE PPSE+SVT+ KPK+DE LN S+ T ES+  GG+ESS NCSN+     E+TAQTSD
Sbjct: 1   MADEGPPSEVSVTVDKPKVDESLNASEVTIESNAQGGVESSSNCSNDKNAATEATAQTSD 60

Query: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120
           GSGEKSLE+AEELLEKGSKA+KDNDF EAVDCFSRALEIRAA YGELAPECVKLYYKYGC
Sbjct: 61  GSGEKSLEMAEELLEKGSKAMKDNDFNEAVDCFSRALEIRAAHYGELAPECVKLYYKYGC 120

Query: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV 180
           ALLYKAQEEADPLGAVPKKEGE HQ+S KD SVKSA NGESSKASVSSNAELVDGV DDV
Sbjct: 121 ALLYKAQEEADPLGAVPKKEGESHQESDKDGSVKSAVNGESSKASVSSNAELVDGVTDDV 180

Query: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240
           SSKKDQDEE+ D+S  EDLA+ADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL
Sbjct: 181 SSKKDQDEEDADNSDAEDLAEADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240

Query: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300
           AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFR+CLCLEFGS+PQEAI FCQ
Sbjct: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRLCLCLEFGSKPQEAIPFCQ 300

Query: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEI 360
           KAISICKSRV+RLTDEVK I+VPTTASSTSGSEP   LSSN SQ D  NAA+EKQSEIE 
Sbjct: 301 KAISICKSRVLRLTDEVKSILVPTTASSTSGSEPAAQLSSNVSQIDTDNAASEKQSEIET 360

Query: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKV-EKIAPPLPAVLNSSQMGSAN 420
           LSGLLVELEKKLEDLQQLASNPKSILSEILGIG+A++KV EK AP  PA LNSSQ+ SAN
Sbjct: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGSARSKVNEKSAP--PAALNSSQLASAN 420

Query: 421 SNGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKG 480
           SNGGFDSPTVSTAHTNGA GVTHLGVVGRGVKRVSTNSESA+ S+P KK A DSS+QDKG
Sbjct: 421 SNGGFDSPTVSTAHTNGAPGVTHLGVVGRGVKRVSTNSESAE-SNPMKKPAIDSSSQDKG 480

Query: 481 DGSSA 485
           DGSSA
Sbjct: 481 DGSSA 482

BLAST of CmaCh20G011420 vs. ExPASy TrEMBL
Match: A0A0A0LMR2 (TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G287190 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 4.8e-205
Identity = 414/488 (84.84%), Postives = 437/488 (89.55%), Query Frame = 0

Query: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD 60
           MADEDPPSE+SVT+ KPKLDE LNVS+ TTES V GGL+SSCN  NE KP  + TAQTSD
Sbjct: 1   MADEDPPSEVSVTVDKPKLDETLNVSEVTTESIVQGGLQSSCNSPNEKKPITQPTAQTSD 60

Query: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120
            SG+KSL+LAEELLEKGSKA+KDNDF EAVDCFSRALEIRAA YGELA ECVKLYYKYGC
Sbjct: 61  ESGDKSLDLAEELLEKGSKAMKDNDFNEAVDCFSRALEIRAAHYGELASECVKLYYKYGC 120

Query: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV 180
           ALLYKAQEEADPLGAVPKKEG    QS KD+SVKSA NGESSKASVSSNAE VDGV DDV
Sbjct: 121 ALLYKAQEEADPLGAVPKKEG----QSDKDDSVKSAVNGESSKASVSSNAEAVDGVTDDV 180

Query: 181 S---SKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDIL 240
           S   SKKD+DEEE D S  EDLADADEDESDLDLAWKMLDVARAIVEKDS DTMEKVDIL
Sbjct: 181 SETVSKKDRDEEESDGSDAEDLADADEDESDLDLAWKMLDVARAIVEKDSADTMEKVDIL 240

Query: 241 SALAEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAIS 300
           SALAEVALEREDI TSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAIS
Sbjct: 241 SALAEVALEREDIGTSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAIS 300

Query: 301 FCQKAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSE 360
           +CQKAISICKSRV+RLTDEVK +IVPTTASSTSGSEPEVPLSSN SQ+D+ NA TEKQSE
Sbjct: 301 YCQKAISICKSRVVRLTDEVKSVIVPTTASSTSGSEPEVPLSSNGSQTDNENATTEKQSE 360

Query: 361 IEILSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGS 420
           I+ LSGLLVELEKKLEDLQQ ASNPKSILSEILGIG+AK  +EKI PP+P+V NSSQMGS
Sbjct: 361 IDTLSGLLVELEKKLEDLQQQASNPKSILSEILGIGSAKPNLEKITPPVPSVFNSSQMGS 420

Query: 421 ANSNGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATD-SSTQ 480
           A+SNGGFDSPTVSTAHTN   GVTHLGVVGRGVKRVSTNSES D S+PTKK A D SS+Q
Sbjct: 421 AHSNGGFDSPTVSTAHTN---GVTHLGVVGRGVKRVSTNSESND-SNPTKKLAKDLSSSQ 480

Query: 481 DKGDGSSA 485
           DKGD SSA
Sbjct: 481 DKGDSSSA 480

BLAST of CmaCh20G011420 vs. NCBI nr
Match: XP_022971716.1 (protein HGV2 [Cucurbita maxima])

HSP 1 Score: 886.7 bits (2290), Expect = 8.9e-254
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD 60
           MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD
Sbjct: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD 60

Query: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120
           GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC
Sbjct: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120

Query: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV 180
           ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV
Sbjct: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV 180

Query: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240
           SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL
Sbjct: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240

Query: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300
           AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ
Sbjct: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300

Query: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEI 360
           KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEI
Sbjct: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEI 360

Query: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 420
           LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS
Sbjct: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 420

Query: 421 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD 480
           NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD
Sbjct: 421 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD 480

Query: 481 GSSA 485
           GSSA
Sbjct: 481 GSSA 484

BLAST of CmaCh20G011420 vs. NCBI nr
Match: KAG7011181.1 (Nuclear autoantigenic sperm protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 879.0 bits (2270), Expect = 1.9e-251
Identity = 479/484 (98.97%), Postives = 483/484 (99.79%), Query Frame = 0

Query: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD 60
           MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGL+SSCNCSNETKPGAESTAQTSD
Sbjct: 36  MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLDSSCNCSNETKPGAESTAQTSD 95

Query: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120
           GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC
Sbjct: 96  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 155

Query: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV 180
           ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVK+ ENGESSKASVSSNAELVDGVADDV
Sbjct: 156 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKNVENGESSKASVSSNAELVDGVADDV 215

Query: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240
           SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL
Sbjct: 216 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 275

Query: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300
           AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ
Sbjct: 276 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 335

Query: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEI 360
           KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQ+D+ANAATEKQSEIEI
Sbjct: 336 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQTDNANAATEKQSEIEI 395

Query: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 420
           LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS
Sbjct: 396 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 455

Query: 421 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD 480
           NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD
Sbjct: 456 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD 515

Query: 481 GSSA 485
           GSSA
Sbjct: 516 GSSA 519

BLAST of CmaCh20G011420 vs. NCBI nr
Match: XP_023513004.1 (LOW QUALITY PROTEIN: protein HGV2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 876.3 bits (2263), Expect = 1.2e-250
Identity = 478/484 (98.76%), Postives = 482/484 (99.59%), Query Frame = 0

Query: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD 60
           MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGL+SSCNCSNETKP AESTAQTSD
Sbjct: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLDSSCNCSNETKPAAESTAQTSD 60

Query: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120
           GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC
Sbjct: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120

Query: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV 180
           ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVK+AENGESSKASVSSNAELVDGVADDV
Sbjct: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKNAENGESSKASVSSNAELVDGVADDV 180

Query: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240
           SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDIL AL
Sbjct: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILXAL 240

Query: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300
           AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ
Sbjct: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300

Query: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEI 360
           KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQ+D+ANAATEKQSEIEI
Sbjct: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQTDNANAATEKQSEIEI 360

Query: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 420
           LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS
Sbjct: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 420

Query: 421 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD 480
           NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD
Sbjct: 421 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD 480

Query: 481 GSSA 485
           GSSA
Sbjct: 481 GSSA 484

BLAST of CmaCh20G011420 vs. NCBI nr
Match: XP_022928044.1 (protein HGV2 [Cucurbita moschata])

HSP 1 Score: 874.0 bits (2257), Expect = 6.0e-250
Identity = 476/484 (98.35%), Postives = 482/484 (99.59%), Query Frame = 0

Query: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD 60
           MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGL+SSCNCSNETKP AESTAQTSD
Sbjct: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLDSSCNCSNETKPAAESTAQTSD 60

Query: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120
           GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC
Sbjct: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120

Query: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV 180
           ALLYKAQEEADPLGAVPKKEGEP+QQSYKDESVK+ ENGESSKASVSSNAELVDGVADDV
Sbjct: 121 ALLYKAQEEADPLGAVPKKEGEPYQQSYKDESVKNVENGESSKASVSSNAELVDGVADDV 180

Query: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240
           SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL
Sbjct: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240

Query: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300
           AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ
Sbjct: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300

Query: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEI 360
           KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPE+PLSSNDSQ+D+ANAATEKQSEIEI
Sbjct: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEIPLSSNDSQTDNANAATEKQSEIEI 360

Query: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 420
           LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS
Sbjct: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 420

Query: 421 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD 480
           NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD
Sbjct: 421 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDKGD 480

Query: 481 GSSA 485
           GSSA
Sbjct: 481 GSSA 484

BLAST of CmaCh20G011420 vs. NCBI nr
Match: KAG6571417.1 (hypothetical protein SDJN03_30332, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 866.7 bits (2238), Expect = 9.5e-248
Identity = 472/478 (98.74%), Postives = 476/478 (99.58%), Query Frame = 0

Query: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTAQTSD 60
           MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGL+SSCNCSNETKPGAESTAQTSD
Sbjct: 1   MADEDPPSELSVTLQKPKLDEILNVSKATTESDVLGGLDSSCNCSNETKPGAESTAQTSD 60

Query: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120
           GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC
Sbjct: 61  GSGEKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGC 120

Query: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKDESVKSAENGESSKASVSSNAELVDGVADDV 180
           ALLYKAQEEADPLGAVPKKEGEPHQQSYK ESVK+ ENGESSKASVSSNAELVDGVADDV
Sbjct: 121 ALLYKAQEEADPLGAVPKKEGEPHQQSYKGESVKNVENGESSKASVSSNAELVDGVADDV 180

Query: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240
           SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL
Sbjct: 181 SSKKDQDEEEGDDSGTEDLADADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILSAL 240

Query: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300
           AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ
Sbjct: 241 AEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISFCQ 300

Query: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEIEI 360
           KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQ+D+ANAATEKQSEIEI
Sbjct: 301 KAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQTDNANAATEKQSEIEI 360

Query: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 420
           LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS
Sbjct: 361 LSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSANS 420

Query: 421 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDK 479
           NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDK
Sbjct: 421 NGGFDSPTVSTAHTNGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRATDSSTQDK 478

BLAST of CmaCh20G011420 vs. TAIR 10
Match: AT4G37210.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 403.3 bits (1035), Expect = 2.8e-112
Identity = 265/493 (53.75%), Postives = 338/493 (68.56%), Query Frame = 0

Query: 5   DPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTA-QTSDGSG 64
           +P +E++ TL+ P L  I    +AT ES V GG ES+CN        A+S A +  D   
Sbjct: 19  EPATEIAQTLE-PNLASI----EATVESVVQGGTESTCNNDANNNNAADSAATEVCDEER 78

Query: 65  EKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGCALL 124
           EK+LE AEEL EKGS  +K+NDF EAVDCFSRALEIR A YGEL  EC+  YY+YG ALL
Sbjct: 79  EKTLEFAEELTEKGSVFLKENDFAEAVDCFSRALEIRVAHYGELDAECINAYYRYGLALL 138

Query: 125 YKAQEEADPLGAVPKKEGEPHQQSYKDESV-KSAENGESSKASVSSNAELVDGVADDVSS 184
            KAQ EADPLG +PKKEGE  Q+S   ES+  S  +G+  +   SS  E         S 
Sbjct: 139 AKAQAEADPLGNMPKKEGEVQQESSNGESLAPSVVSGDPERQGSSSGQE--------GSG 198

Query: 185 KKDQDEEEGDDSGTEDLA----DADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILS 244
            KDQ  E+G+D   +DL+    DADEDESDLD+AWKMLD+AR I +K S +TMEKVDIL 
Sbjct: 199 GKDQG-EDGEDCQDDDLSDADGDADEDESDLDMAWKMLDIARVITDKQSTETMEKVDILC 258

Query: 245 ALAEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISF 304
           +LAEV+LEREDIE+SLSDY+ ALSILERLVEPD+R+ AELNFR+C+CLE G QP+EAI +
Sbjct: 259 SLAEVSLEREDIESSLSDYKNALSILERLVEPDSRRTAELNFRICICLETGCQPKEAIPY 318

Query: 305 CQKAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEI 364
           CQKA+ ICK+R+ RL++E+KG     T+S+ S  +  +  SSN    D   +A++K+ EI
Sbjct: 319 CQKALLICKARMERLSNEIKGASGSATSSTVSEIDEGIQQSSNVPYID--KSASDKEVEI 378

Query: 365 EILSGLLVELEKKLEDLQQLASNPKSILSEILGIGAAKAKVEKIAPPLPAVLNSSQMGSA 424
             L+GL  +LEKKLEDL+Q A NPK +L+E++G+ +AK        P  A ++SS+MG+ 
Sbjct: 379 GDLAGLAEDLEKKLEDLKQQAENPKQVLAELMGMVSAKPNASDKVVPAAAEMSSSRMGTV 438

Query: 425 NSNGGFD--SPTVSTAHT-----NGAAGVTHLGVVGRGVKRVSTNSESADSSHPTKKRAT 484
           N+N G D  SPTVSTAHT       A+GVTHLGVVGRGVKRV  N+ S +SS  +KK A 
Sbjct: 439 NTNFGKDLESPTVSTAHTGAAGGGAASGVTHLGVVGRGVKRVLMNTTSIESS-ASKKPAL 492

BLAST of CmaCh20G011420 vs. TAIR 10
Match: AT4G37210.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 319.3 bits (817), Expect = 5.3e-87
Identity = 203/373 (54.42%), Postives = 257/373 (68.90%), Query Frame = 0

Query: 5   DPPSELSVTLQKPKLDEILNVSKATTESDVLGGLESSCNCSNETKPGAESTA-QTSDGSG 64
           +P +E++ TL+ P L  I    +AT ES V GG ES+CN        A+S A +  D   
Sbjct: 19  EPATEIAQTLE-PNLASI----EATVESVVQGGTESTCNNDANNNNAADSAATEVCDEER 78

Query: 65  EKSLELAEELLEKGSKAIKDNDFVEAVDCFSRALEIRAARYGELAPECVKLYYKYGCALL 124
           EK+LE AEEL EKGS  +K+NDF EAVDCFSRALEIR A YGEL  EC+  YY+YG ALL
Sbjct: 79  EKTLEFAEELTEKGSVFLKENDFAEAVDCFSRALEIRVAHYGELDAECINAYYRYGLALL 138

Query: 125 YKAQEEADPLGAVPKKEGEPHQQSYKDESV-KSAENGESSKASVSSNAELVDGVADDVSS 184
            KAQ EADPLG +PKKEGE  Q+S   ES+  S  +G+  +   SS  E         S 
Sbjct: 139 AKAQAEADPLGNMPKKEGEVQQESSNGESLAPSVVSGDPERQGSSSGQE--------GSG 198

Query: 185 KKDQDEEEGDDSGTEDLA----DADEDESDLDLAWKMLDVARAIVEKDSGDTMEKVDILS 244
            KDQ  E+G+D   +DL+    DADEDESDLD+AWKMLD+AR I +K S +TMEKVDIL 
Sbjct: 199 GKDQG-EDGEDCQDDDLSDADGDADEDESDLDMAWKMLDIARVITDKQSTETMEKVDILC 258

Query: 245 ALAEVALEREDIETSLSDYQKALSILERLVEPDNRQLAELNFRVCLCLEFGSQPQEAISF 304
           +LAEV+LEREDIE+SLSDY+ ALSILERLVEPD+R+ AELNFR+C+CLE G QP+EAI +
Sbjct: 259 SLAEVSLEREDIESSLSDYKNALSILERLVEPDSRRTAELNFRICICLETGCQPKEAIPY 318

Query: 305 CQKAISICKSRVMRLTDEVKGIIVPTTASSTSGSEPEVPLSSNDSQSDHANAATEKQSEI 364
           CQKA+ ICK+R+ RL++E+KG     T+S+ S  +  +  SSN    D   +A++K+ EI
Sbjct: 319 CQKALLICKARMERLSNEIKGASGSATSSTVSEIDEGIQQSSNVPYID--KSASDKEVEI 375

Query: 365 EILSGLLVELEKK 372
             L+GL  +LEKK
Sbjct: 379 GDLAGLAEDLEKK 375

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q178866.9e-0721.53Protein NASP homolog 1 OS=Caenorhabditis elegans OX=6239 GN=nasp-1 PE=1 SV=1[more]
Q2T9P41.5e-0626.54Nuclear autoantigenic sperm protein OS=Bos taurus OX=9913 GN=NASP PE=2 SV=2[more]
Q66HD39.9e-0622.83Nuclear autoantigenic sperm protein OS=Rattus norvegicus OX=10116 GN=Nasp PE=1 S... [more]
P061802.9e-0522.88Histone-binding protein N1/N2 OS=Xenopus laevis OX=8355 PE=1 SV=3[more]
Q9USQ44.2e-0423.72NASP-related protein sim3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843)... [more]
Match NameE-valueIdentityDescription
A0A6J1I4124.3e-254100.00protein HGV2 OS=Cucurbita maxima OX=3661 GN=LOC111470382 PE=4 SV=1[more]
A0A6J1EJQ12.9e-25098.35protein HGV2 OS=Cucurbita moschata OX=3662 GN=LOC111434946 PE=4 SV=1[more]
A0A1S3C6L85.5e-20986.04NASP-related protein sim3 OS=Cucumis melo OX=3656 GN=LOC103497438 PE=4 SV=1[more]
A0A6J1E0531.6e-20885.36NASP-related protein sim3 OS=Momordica charantia OX=3673 GN=LOC111025157 PE=4 SV... [more]
A0A0A0LMR24.8e-20584.84TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G287190 ... [more]
Match NameE-valueIdentityDescription
XP_022971716.18.9e-254100.00protein HGV2 [Cucurbita maxima][more]
KAG7011181.11.9e-25198.97Nuclear autoantigenic sperm protein, partial [Cucurbita argyrosperma subsp. argy... [more]
XP_023513004.11.2e-25098.76LOW QUALITY PROTEIN: protein HGV2 [Cucurbita pepo subsp. pepo][more]
XP_022928044.16.0e-25098.35protein HGV2 [Cucurbita moschata][more]
KAG6571417.19.5e-24898.74hypothetical protein SDJN03_30332, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT4G37210.12.8e-11253.75Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G37210.25.3e-8754.42Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 247..267
NoneNo IPR availableCOILSCoilCoilcoord: 351..381
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..157
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 190..205
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 324..351
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 324..354
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..205
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..64
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 414..484
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 414..437
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..66
NoneNo IPR availablePANTHERPTHR15081:SF1NUCLEAR AUTOANTIGENIC SPERM PROTEINcoord: 52..480
NoneNo IPR availablePANTHERPTHR15081NUCLEAR AUTOANTIGENIC SPERM PROTEIN NASP -RELATEDcoord: 52..480
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 234..267
e-value: 4.2
score: 16.4
coord: 70..103
e-value: 5.0
score: 16.1
coord: 276..309
e-value: 140.0
score: 4.8
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 54..378
e-value: 1.1E-22
score: 82.8
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 65..308

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G011420.1CmaCh20G011420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034080 CENP-A containing chromatin assembly
biological_process GO:0006335 DNA replication-dependent chromatin assembly
cellular_component GO:0005654 nucleoplasm
molecular_function GO:0042393 histone binding
molecular_function GO:0005515 protein binding