Homology
BLAST of CmaCh20G009760 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 226.1 bits (575), Expect = 5.0e-58
Identity = 132/359 (36.77%), Postives = 190/359 (52.92%), Query Frame = 0
Query: 12 RPSTKLPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGHVFYLRLGSQLVVIVSSPSAV 71
R LPPSP +LP+I HL+L+ + +H++L LSKKYG VF L+LG++LV++VSSP+A
Sbjct: 27 RRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGSVFSLQLGNRLVLVVSSPAAA 86
Query: 72 KECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTIEIFSATRLNMFL 131
+ECFT NDI+ AN+P + K++ YN T SPYG+ WR LRR++ +EIFSA LN FL
Sbjct: 87 EECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWRNLRRLAAVEIFSAGSLNRFL 146
Query: 132 ETRRDEIKHLL-------------ALMGSMISELSSNVVMRMVMGKRFYGEG-DLEQARK 191
R DE+K LL M S +SELS NV MRMV GKR++G+ D ++A+
Sbjct: 147 SIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTMRMVAGKRYFGQDVDSDEAKL 206
Query: 192 FRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR------------------------- 251
FR ++ +V + G+ + +F+P L WID +YEK+
Sbjct: 207 FRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVSKISQEMDAFLQRLIHESRINKNN 266
Query: 252 -----------------RNDEFLQGI---------------------------------- 280
D+ ++GI
Sbjct: 267 VTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKAR 326
BLAST of CmaCh20G009760 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 214.9 bits (546), Expect = 1.2e-54
Identity = 143/384 (37.24%), Postives = 192/384 (50.00%), Query Frame = 0
Query: 1 MAFISALIFLL----------RPSTKLPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYG 60
++ I +L FL+ R LPP P LPII +L+ LK+P+H T LS+KYG
Sbjct: 6 LSLIISLFFLIITLKVFFNTSRKFKNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTLSQKYG 65
Query: 61 HVFYLRLGSQLVVIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRW 120
+F L GS+LVV+VSS + +ECFT NDI+LAN+P K++ YNNT + SPYGD W
Sbjct: 66 QIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHW 125
Query: 121 RELRRISTIEIFSATRLNMFLETRRDEIKHLLALMGS-------------MISELSSNVV 180
R LRRI +IEI S+ RLN FLE RRDEI L+ + M SE++ N +
Sbjct: 126 RNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTI 185
Query: 181 MRMVMGKRFYGE----GDLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR- 240
MRMV GKR+YG D+E+AR FR I+K+V+ G+ + +F+ L W D EKR
Sbjct: 186 MRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRL 245
Query: 241 -----RNDEFLQGI---------------------------------------------- 280
R D FLQG+
Sbjct: 246 KKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGT 305
BLAST of CmaCh20G009760 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 213.4 bits (542), Expect = 3.4e-54
Identity = 135/383 (35.25%), Postives = 202/383 (52.74%), Query Frame = 0
Query: 1 MAFISALIFLLRPSTK----LPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGHVFYLR 60
++FI + LL+ +++ LPP PP ++PII +L+ LK P+H+T LS+ YG +F L
Sbjct: 10 LSFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLW 69
Query: 61 LGSQLVVIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRI 120
GS+LVV+VSSPS ECFT NDIILAN+P K++ YN T + YGD WR LRRI
Sbjct: 70 FGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRI 129
Query: 121 STIEIFSATRLNMFLETRRDEIKHLL-ALMGSMIS---------------ELSSNVVMRM 180
+TI++ S RLN FL RRDE L+ L+ ++S E++ N +MRM
Sbjct: 130 TTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMRM 189
Query: 181 VMGKRFYGE----GDLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR---- 240
+ GKR+YG+ D+E+A++FR+I+ ++M G+ + +F+PLL +D+ + EKR
Sbjct: 190 ISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRI 249
Query: 241 --RNDEFLQGI------------------------------------------------- 280
R++ FL+G+
Sbjct: 250 AKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTD 309
BLAST of CmaCh20G009760 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 212.6 bits (540), Expect = 5.7e-54
Identity = 132/372 (35.48%), Postives = 194/372 (52.15%), Query Frame = 0
Query: 3 FISALIFLLRPSTKLPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGHVFYLRLGSQLV 62
FI ++ R LPP PP SLPII +L+ LKRP+H+T + LS+KYGHVF L GS+LV
Sbjct: 18 FIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLV 77
Query: 63 VIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTIEIF 122
V+VSS S ++CFT ND++LAN+P K++ YN T + YG+ WR LRRI+ +++
Sbjct: 78 VVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVL 137
Query: 123 SATRLNMFLETRRDEIKHLLAL-------------MGSMISELSSNVVMRMVMGKRFYGE 182
S R+N F RRDE + L+ + S + +++ N +MRM+ GKR+YGE
Sbjct: 138 SNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGE 197
Query: 183 ----GDLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR------RNDEFLQ 242
DL++A +FRD+V +++Q G+ + +F+PLL ++D + EKR + D FL+
Sbjct: 198 DCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLR 257
Query: 243 GI---------------------------------------------------------- 280
G+
Sbjct: 258 GLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMS 317
BLAST of CmaCh20G009760 vs. ExPASy Swiss-Prot
Match:
Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 201.8 bits (512), Expect = 1.0e-50
Identity = 139/382 (36.39%), Postives = 193/382 (50.52%), Query Frame = 0
Query: 4 ISALIFL------LRPSTK-LPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGH----- 63
I +LIFL L+P + LPPSPP LPII HL LLK P+H+TL+ S+ H
Sbjct: 13 IFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGG 72
Query: 64 VFYLRLGSQLVVIVSSPS-AVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRW 123
V LRLGS+LV +VSS A +ECF ND++LAN+P I KH+ YNNT +PYGD W
Sbjct: 73 VMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHW 132
Query: 124 RELRRISTIEIFSATRLNMFLETRRDEIKHLLALMG-------------SMISELSSNVV 183
R LRR+ TIEIFS RLN FL R DE++ L++ + M+ +L+ N +
Sbjct: 133 RNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNI 192
Query: 184 MRMVMGKRFYGE--GDLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR--- 243
MRM+ GKR+YGE D E+A++ R +V V S ++ +++P+L + YE R
Sbjct: 193 MRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRL--FSSYENRVKK 252
Query: 244 ---RNDEFLQGI------------------------------------------------ 280
D+FLQG+
Sbjct: 253 LGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIAGTNT 312
BLAST of CmaCh20G009760 vs. ExPASy TrEMBL
Match:
A0A6J1FUI0 (cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448921 PE=3 SV=1)
HSP 1 Score: 443.7 bits (1140), Expect = 5.7e-121
Identity = 251/373 (67.29%), Postives = 264/373 (70.78%), Query Frame = 0
Query: 1 MAFISALIFLLRPSTKLPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGHVFYLRLGSQ 60
MAFIS+LIFLLRPSTKLPPSPPFSLPII HLYLLKRPVHQTLQKLS KYGHVF LRLGSQ
Sbjct: 13 MAFISSLIFLLRPSTKLPPSPPFSLPIIGHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQ 72
Query: 61 LVVIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTIE 120
LVV+VSSPSAVKECFTMNDIILAN+PSSNIIKHLTYNNTIFS S YGD WRELRRISTIE
Sbjct: 73 LVVVVSSPSAVKECFTMNDIILANRPSSNIIKHLTYNNTIFSASSYGDHWRELRRISTIE 132
Query: 121 IFSATRLNMFLETRRDEIKHLLAL-------------MGSMISELSSNVVMRMVMGKRFY 180
IFSA+RLNMFLETR+DEIK +L M SMISELSSN+VMRMVMGKRFY
Sbjct: 133 IFSASRLNMFLETRKDEIKRVLGKLCRISSDSFARVEMSSMISELSSNIVMRMVMGKRFY 192
Query: 181 GEG--DLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR------RNDEFLQ 240
GEG DLEQARKFRDIVKQVMQKCGSFDSR+FIPLLNWIDITHYEK+ RNDEFLQ
Sbjct: 193 GEGVLDLEQARKFRDIVKQVMQKCGSFDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQ 252
Query: 241 G----------------------------------------------------------- 280
G
Sbjct: 253 GLIDEHRNQKEEGRNTMIDHLLSLQQSQLDFMSDQVIKGLIMVILLAGTDTIAVTIEWAL 312
BLAST of CmaCh20G009760 vs. ExPASy TrEMBL
Match:
A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)
HSP 1 Score: 274.6 bits (701), Expect = 4.5e-70
Identity = 163/365 (44.66%), Postives = 211/365 (57.81%), Query Frame = 0
Query: 9 FLLRPSTK-LPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGHVFYLRLGSQLVVIVSS 68
FLLRP + LPPSP F LP+I HL+L+K PVH+TL KLS YGHVF LR GS+LVV+VSS
Sbjct: 101 FLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSS 160
Query: 69 PSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTIEIFSATRL 128
PSAV+ECFT NDI+LAN+P K L YN+T +VSPYG+ WR LRRIST+EIFS +RL
Sbjct: 161 PSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRL 220
Query: 129 NMFLETRRDEIKHLL-------------ALMGSMISELSSNVVMRMVMGKRFYGEG--DL 188
N+FL R DE+K+LL + SM+ + + N+V+RM+ GK++YGE D
Sbjct: 221 NLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDE 280
Query: 189 EQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR------RNDEFLQGI----- 248
Q++KFR++VK+++ G+ + +F+PL NWID T YEKR R+DE LQ +
Sbjct: 281 GQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIR 340
Query: 249 ------------------------------------------------------------ 280
Sbjct: 341 NQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPE 400
BLAST of CmaCh20G009760 vs. ExPASy TrEMBL
Match:
A0A7N2M2B9 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)
HSP 1 Score: 273.9 bits (699), Expect = 7.7e-70
Identity = 166/374 (44.39%), Postives = 211/374 (56.42%), Query Frame = 0
Query: 6 ALIFLLRPSTK---LPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGHVFYLRLGSQLV 65
A FLLR TK PPSPP SLPI+ HL+L+K+P+H+TL S+KYG++F LR GSQLV
Sbjct: 19 AFKFLLRTRTKHKHHPPSPP-SLPILGHLHLIKKPLHRTLYHFSQKYGNIFSLRFGSQLV 78
Query: 66 VIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTIEIF 125
VIVSSPSAV+ECFT NDIILAN+P K++TYN+T + + YGD WR LRRIST+EIF
Sbjct: 79 VIVSSPSAVEECFTKNDIILANRPLFPATKYITYNHTTLATTSYGDHWRNLRRISTLEIF 138
Query: 126 SATRLNMFLETRRDEIKHLLALMG-------------SMISELSSNVVMRMVMGKRFYGE 185
S RLNMFL RRDE+KHLL + SM SEL+ N++MRMV GKR+YGE
Sbjct: 139 STNRLNMFLGIRRDEVKHLLCKLSRNSCQGFAKVELKSMFSELTLNIIMRMVAGKRYYGE 198
Query: 186 G--DLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR------RNDEFLQG- 245
D E+AR+F I+K+++ G + ++F+PLL WID EKR R D FLQG
Sbjct: 199 DMKDEEEARQFMGIMKEIIGSAGPSNPQDFVPLLRWIDRRRLEKRLMKLFDRADAFLQGL 258
Query: 246 ------------------------------------------------------------ 284
Sbjct: 259 IDEKRSKEEKGNTIIDHLLSLQKSQPEYYTDQIIKGLIQVLLSAGTNTSTMTLEWAMSNL 318
BLAST of CmaCh20G009760 vs. ExPASy TrEMBL
Match:
A0A6J1FSK8 (cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448412 PE=3 SV=1)
HSP 1 Score: 273.5 bits (698), Expect = 1.0e-69
Identity = 163/369 (44.17%), Postives = 212/369 (57.45%), Query Frame = 0
Query: 1 MAFISALIFLLRPSTKLPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGHVFYLRLGSQ 60
+ F+ AL F R LPP+P FSLPII HL+LLK P+H+TL LS K+GHVF LR GS+
Sbjct: 13 ITFLLALKFFPRHVKNLPPTPLFSLPIIGHLHLLKHPIHRTLHHLSNKHGHVFSLRFGSR 72
Query: 61 LVVIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTIE 120
LVV++SSPSAV+ECFT NDI+LAN+P N KHLTYN T+ +V+PYG+ WR LRRIST E
Sbjct: 73 LVVVISSPSAVRECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGELWRTLRRISTRE 132
Query: 121 IFSATRLNMFLETRRDEIKHLLALM----------GSMISELSSNVVMRMVMGKRFYGE- 180
IFS TRL++ R+DE+K LL + MI +L+SN+VMRMV GKR++G+
Sbjct: 133 IFSTTRLDLSSGIRQDEVKRLLCKLCRDDNVVVEVQPMIMDLTSNIVMRMVAGKRYFGDD 192
Query: 181 -GDLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR---------------- 240
D EQA +FRD+VK+VM G+ + +FIPL NWID + +EK+
Sbjct: 193 VSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKKIMKVGEEADAIFNGLI 252
Query: 241 ---RN------------------------DEFLQGI------------------------ 280
RN D+ ++G+
Sbjct: 253 DEIRNQKGSGNTMIDHLLHLQATQSEYFSDQIIKGLIHVILLAGIDTTAVTLEWALSHLL 312
BLAST of CmaCh20G009760 vs. ExPASy TrEMBL
Match:
A0A0A0LTD0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1)
HSP 1 Score: 273.1 bits (697), Expect = 1.3e-69
Identity = 165/376 (43.88%), Postives = 211/376 (56.12%), Query Frame = 0
Query: 1 MAFISALIFLLRPSTKLPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGHVFYLRLGSQ 60
++ + L LL LPP+P FSLP+I HL+LLK P+HQTL LSKKYGHVF LR GS
Sbjct: 14 ISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSKKYGHVFSLRFGSH 73
Query: 61 LVVIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTIE 120
LVV++SSPSAV+ECFT NDIILAN+P N KHL+YN T+ +V+PYG+ WR LRRIST E
Sbjct: 74 LVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGELWRRLRRISTCE 133
Query: 121 IFSATRLNMFLETRRDEIKHLL-----------------ALMGSMISELSSNVVMRMVMG 180
IFS ++LN F R+DE+K LL + ++ + +SN+VMRMV G
Sbjct: 134 IFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILLDFTSNIVMRMVGG 193
Query: 181 KRFYGEGDLE--QARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR--------- 240
KR++G+ L+ QA KFRD+VK+VM G+ + +FIPL NWID T EK+
Sbjct: 194 KRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEKKIMKVGEEAD 253
Query: 241 ----------RNDE---------------------------------FLQGI-------- 280
RN+E FL GI
Sbjct: 254 EIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVIFLAGIDTISVTLE 313
BLAST of CmaCh20G009760 vs. NCBI nr
Match:
XP_023513339.1 (cytochrome P450 81D1-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 457.6 bits (1176), Expect = 7.8e-125
Identity = 258/373 (69.17%), Postives = 266/373 (71.31%), Query Frame = 0
Query: 1 MAFISALIFLLRPSTKLPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGHVFYLRLGSQ 60
MAFISALIFLLRPSTKLPPSPPFSLPII HLYLLKRPVHQTLQKLS KYGHVF LRLGSQ
Sbjct: 13 MAFISALIFLLRPSTKLPPSPPFSLPIIGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQ 72
Query: 61 LVVIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTIE 120
LVV+VSSPS VKECFTMNDIILAN+PSSNIIKHLTYNNTIFS SPYGD WRELRRISTIE
Sbjct: 73 LVVVVSSPSVVKECFTMNDIILANRPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIE 132
Query: 121 IFSATRLNMFLETRRDEIKHLLAL-------------MGSMISELSSNVVMRMVMGKRFY 180
IFSATRLNMFLETR+DEIKHLL M SMISELSSN+VMRMVMGKRFY
Sbjct: 133 IFSATRLNMFLETRKDEIKHLLGKLCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFY 192
Query: 181 GEG--DLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR------RNDEFLQ 240
GEG DLEQARKFRDIVKQVMQKCGSFDSR+FIPLLNWIDITHYEKR RNDEFLQ
Sbjct: 193 GEGVSDLEQARKFRDIVKQVMQKCGSFDSRSFIPLLNWIDITHYEKRIIKLGQRNDEFLQ 252
Query: 241 G----------------------------------------------------------- 280
G
Sbjct: 253 GLIDEHRNQKEEGRNTMIDHLLSLQQSQLDFMSDQVIKGLITVILLAGTDTIAVTIEWAL 312
BLAST of CmaCh20G009760 vs. NCBI nr
Match:
XP_022944471.1 (cytochrome P450 81D1-like [Cucurbita moschata])
HSP 1 Score: 443.7 bits (1140), Expect = 1.2e-120
Identity = 251/373 (67.29%), Postives = 264/373 (70.78%), Query Frame = 0
Query: 1 MAFISALIFLLRPSTKLPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGHVFYLRLGSQ 60
MAFIS+LIFLLRPSTKLPPSPPFSLPII HLYLLKRPVHQTLQKLS KYGHVF LRLGSQ
Sbjct: 13 MAFISSLIFLLRPSTKLPPSPPFSLPIIGHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQ 72
Query: 61 LVVIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTIE 120
LVV+VSSPSAVKECFTMNDIILAN+PSSNIIKHLTYNNTIFS S YGD WRELRRISTIE
Sbjct: 73 LVVVVSSPSAVKECFTMNDIILANRPSSNIIKHLTYNNTIFSASSYGDHWRELRRISTIE 132
Query: 121 IFSATRLNMFLETRRDEIKHLLAL-------------MGSMISELSSNVVMRMVMGKRFY 180
IFSA+RLNMFLETR+DEIK +L M SMISELSSN+VMRMVMGKRFY
Sbjct: 133 IFSASRLNMFLETRKDEIKRVLGKLCRISSDSFARVEMSSMISELSSNIVMRMVMGKRFY 192
Query: 181 GEG--DLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR------RNDEFLQ 240
GEG DLEQARKFRDIVKQVMQKCGSFDSR+FIPLLNWIDITHYEK+ RNDEFLQ
Sbjct: 193 GEGVLDLEQARKFRDIVKQVMQKCGSFDSRSFIPLLNWIDITHYEKKIIKLGQRNDEFLQ 252
Query: 241 G----------------------------------------------------------- 280
G
Sbjct: 253 GLIDEHRNQKEEGRNTMIDHLLSLQQSQLDFMSDQVIKGLIMVILLAGTDTIAVTIEWAL 312
BLAST of CmaCh20G009760 vs. NCBI nr
Match:
XP_038902550.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 388.7 bits (997), Expect = 4.5e-104
Identity = 222/371 (59.84%), Postives = 246/371 (66.31%), Query Frame = 0
Query: 3 FISALIFLLRPSTKLPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGHVFYLRLGSQLV 62
FISAL FL++PS LPPSPPFSLPII HLYLLKRP+HQTLQKLSKKYGHVF LRLGSQLV
Sbjct: 15 FISALKFLVKPSINLPPSPPFSLPIIGHLYLLKRPIHQTLQKLSKKYGHVFSLRLGSQLV 74
Query: 63 VIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTIEIF 122
V+VSSPSAVKECFT+NDI LAN+P +NI KHLTYNNTIFS S YGD WR+LRRISTIEIF
Sbjct: 75 VVVSSPSAVKECFTVNDITLANRPQTNITKHLTYNNTIFSASSYGDHWRDLRRISTIEIF 134
Query: 123 SATRLNMFLETRRDEIKHLLAL-------------MGSMISELSSNVVMRMVMGKRFYGE 182
S TRLNMFLE R+DEIK LL MGSM S+LSSN+VMRMV GKRFYGE
Sbjct: 135 STTRLNMFLEIRKDEIKRLLGKLCGISSDDFASVEMGSMFSKLSSNIVMRMVTGKRFYGE 194
Query: 183 G--DLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR------RNDEFLQG- 242
D EQA++FRDIVKQVM+ CGS+DS + + LLNWIDIT YEKR RND+FLQG
Sbjct: 195 DVLDAEQAKEFRDIVKQVMENCGSYDSSSIL-LLNWIDITGYEKRIIELGQRNDQFLQGL 254
Query: 243 ------------------------------------------------------------ 280
Sbjct: 255 IDEHRNQKEEGRNTMIDHLLSLQESQLDCMSDQVIKGLIMVILLAGTDTVAVTMEWALSH 314
BLAST of CmaCh20G009760 vs. NCBI nr
Match:
XP_022149460.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])
HSP 1 Score: 274.6 bits (701), Expect = 9.4e-70
Identity = 163/365 (44.66%), Postives = 211/365 (57.81%), Query Frame = 0
Query: 9 FLLRPSTK-LPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGHVFYLRLGSQLVVIVSS 68
FLLRP + LPPSP F LP+I HL+L+K PVH+TL KLS YGHVF LR GS+LVV+VSS
Sbjct: 101 FLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSS 160
Query: 69 PSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTIEIFSATRL 128
PSAV+ECFT NDI+LAN+P K L YN+T +VSPYG+ WR LRRIST+EIFS +RL
Sbjct: 161 PSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRL 220
Query: 129 NMFLETRRDEIKHLL-------------ALMGSMISELSSNVVMRMVMGKRFYGEG--DL 188
N+FL R DE+K+LL + SM+ + + N+V+RM+ GK++YGE D
Sbjct: 221 NLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDE 280
Query: 189 EQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR------RNDEFLQGI----- 248
Q++KFR++VK+++ G+ + +F+PL NWID T YEKR R+DE LQ +
Sbjct: 281 GQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIR 340
Query: 249 ------------------------------------------------------------ 280
Sbjct: 341 NQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPE 400
BLAST of CmaCh20G009760 vs. NCBI nr
Match:
XP_030926044.1 (cytochrome P450 81E8-like [Quercus lobata])
HSP 1 Score: 273.9 bits (699), Expect = 1.6e-69
Identity = 166/374 (44.39%), Postives = 211/374 (56.42%), Query Frame = 0
Query: 6 ALIFLLRPSTK---LPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGHVFYLRLGSQLV 65
A FLLR TK PPSPP SLPI+ HL+L+K+P+H+TL S+KYG++F LR GSQLV
Sbjct: 19 AFKFLLRTRTKHKHHPPSPP-SLPILGHLHLIKKPLHRTLYHFSQKYGNIFSLRFGSQLV 78
Query: 66 VIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTIEIF 125
VIVSSPSAV+ECFT NDIILAN+P K++TYN+T + + YGD WR LRRIST+EIF
Sbjct: 79 VIVSSPSAVEECFTKNDIILANRPLFPATKYITYNHTTLATTSYGDHWRNLRRISTLEIF 138
Query: 126 SATRLNMFLETRRDEIKHLLALMG-------------SMISELSSNVVMRMVMGKRFYGE 185
S RLNMFL RRDE+KHLL + SM SEL+ N++MRMV GKR+YGE
Sbjct: 139 STNRLNMFLGIRRDEVKHLLCKLSRNSCQGFAKVELKSMFSELTLNIIMRMVAGKRYYGE 198
Query: 186 G--DLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR------RNDEFLQG- 245
D E+AR+F I+K+++ G + ++F+PLL WID EKR R D FLQG
Sbjct: 199 DMKDEEEARQFMGIMKEIIGSAGPSNPQDFVPLLRWIDRRRLEKRLMKLFDRADAFLQGL 258
Query: 246 ------------------------------------------------------------ 284
Sbjct: 259 IDEKRSKEEKGNTIIDHLLSLQKSQPEYYTDQIIKGLIQVLLSAGTNTSTMTLEWAMSNL 318
BLAST of CmaCh20G009760 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 210.3 bits (534), Expect = 2.0e-54
Identity = 133/368 (36.14%), Postives = 194/368 (52.72%), Query Frame = 0
Query: 7 LIFLLRPSTKLPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGH--VFYLRLGSQLVVI 66
LI L+ LPPSP +SLP+I HL LLK P+H+T LS+ + +F LRLG++LV +
Sbjct: 20 LIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIFSLRLGNRLVFV 79
Query: 67 VSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTIEIFSA 126
SS S +ECFT ND++LAN+P+ + KH+ Y+ T + YGD WR LRRI ++EIFS
Sbjct: 80 NSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNLRRIGSVEIFSN 139
Query: 127 TRLNMFLETRRDEIKHLL-------------ALMGSMISELSSNVVMRMVMGKRFYGEG- 186
RLN FL R+DEI+ L+ M SM+S+L+ N ++RMV GKR+YG+G
Sbjct: 140 HRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRMVAGKRYYGDGV 199
Query: 187 -DLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR------RNDEFLQGI-- 246
D +A++ R ++ V+ G+ ++ +++P+L ++ YE R R DEFLQG+
Sbjct: 200 EDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRL--VSDYETRVKKLAGRLDEFLQGLVD 259
Query: 247 ------------------------------------------------------------ 280
Sbjct: 260 EKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTSAVTLEWALSNVLN 319
BLAST of CmaCh20G009760 vs. TAIR 10
Match:
AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )
HSP 1 Score: 209.1 bits (531), Expect = 4.5e-54
Identity = 127/371 (34.23%), Postives = 193/371 (52.02%), Query Frame = 0
Query: 4 ISALIFLLRPSTK--LPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGH--VFYLRLGS 63
IS + L + + K LPPSP + LP+I HL+LLK+PVH+T +SK G+ +F+LRLG+
Sbjct: 16 ISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPIFHLRLGN 75
Query: 64 QLVVIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRISTI 123
+LV ++SS S +ECFT ND++LAN+P + KH+ YN T + YGD WR LRRI+ +
Sbjct: 76 RLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNLRRIAAV 135
Query: 124 EIFSATRLNMFLETRRDEIKHLLALMG-------------SMISELSSNVVMRMVMGKRF 183
EIFS+ R++ F R+DEI+ L+ + S+++ L+ N ++ MV GKR+
Sbjct: 136 EIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNIIMMVAGKRY 195
Query: 184 YGEG--DLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNW--------------------- 243
YG G D ++A+ R+++ ++M GS + +++P +NW
Sbjct: 196 YGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWVTNFENQTKILGNRLDRVLQK 255
Query: 244 -IDITHYEKRR-------------------NDEFLQGI---------------------- 280
+D EK + D ++GI
Sbjct: 256 LVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLAGTDTSSVTLEWAMSN 315
BLAST of CmaCh20G009760 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 207.6 bits (527), Expect = 1.3e-53
Identity = 130/374 (34.76%), Postives = 194/374 (51.87%), Query Frame = 0
Query: 1 MAFISALIFLLRPSTK---LPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGH--VFYL 60
++ + IFLL S + LPPSP SLP+I HL+LLK P+H+T LSK G+ VF+L
Sbjct: 12 LSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAPVFHL 71
Query: 61 RLGSQLVVIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRR 120
RLG++LV ++SS S +ECFT ND++LAN+P I KHL YN T + YGD WR LRR
Sbjct: 72 RLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWRNLRR 131
Query: 121 ISTIEIFSATRLNMFLETRRDEIKHLLA-------------LMGSMISELSSNVVMRMVM 180
I+ +EIFS RLN FL R+DEI+ L++ M ++++ L+SN +RM+
Sbjct: 132 IAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNTTIRMLA 191
Query: 181 GKRFYGEGDLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR------RNDE 240
GKR++GE D + A+ +++V + + G+ + +++ +L W ++ YEKR R D
Sbjct: 192 GKRYFGE-DNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRW--VSSYEKRIKNLGNRFDT 251
Query: 241 FLQGI------------------------------------------------------- 279
FLQ +
Sbjct: 252 FLQKLVDEKRAEKEKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIAGTDTSSVTLEW 311
BLAST of CmaCh20G009760 vs. TAIR 10
Match:
AT2G23190.1 (cytochrome P450, family 81, subfamily D, polypeptide 7 )
HSP 1 Score: 205.3 bits (521), Expect = 6.5e-53
Identity = 136/378 (35.98%), Postives = 192/378 (50.79%), Query Frame = 0
Query: 3 FIS-ALIFLLRPST-KLPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYGH--VFYLRLG 62
FIS L+F R S LPPSP LP I HL+LLK+P+H+T S+ G +F LRLG
Sbjct: 59 FISLKLLFGKRHSKFNLPPSPARPLPFIGHLHLLKQPLHRTFLSFSQSLGDAPIFSLRLG 118
Query: 63 SQLVVIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRIST 122
+ L V+VSS S +ECFT NDI+LAN+P + KH+ YN T + +PYGD WR LRRI T
Sbjct: 119 NHLTVVVSSYSIAEECFTKNDIVLANRPKFILGKHIEYNFTTMTSAPYGDHWRNLRRIGT 178
Query: 123 IEIFSATRLNMFLETRRDEIKHLL-------------ALMGSMISELSSNVVMRMVMGKR 182
+EIFS+ +LN FL R+DEI+HLL M + +L+ N ++RMV GKR
Sbjct: 179 LEIFSSHKLNGFLSVRKDEIRHLLLRLSKNSQHGFAKVEMRQLFYDLTINNILRMVAGKR 238
Query: 183 FYGEG--DLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEK------RRNDEF 242
FYGEG E AR+ ++ +++ + G ++ ++IP+L W IT +EK R DEF
Sbjct: 239 FYGEGTEQDEVARRVTQLIDEIVYRAGVGNAADYIPILRW--ITDFEKGVKELASRVDEF 298
Query: 243 LQGI-------------------------------------------------------- 284
LQ +
Sbjct: 299 LQSLVDERRVHKQKGNTMMDHLLSLQETQPDYYTDVTLKGIIIVMILAGTETLAGTLEWA 358
BLAST of CmaCh20G009760 vs. TAIR 10
Match:
AT2G23220.1 (cytochrome P450, family 81, subfamily D, polypeptide 6 )
HSP 1 Score: 204.5 bits (519), Expect = 1.1e-52
Identity = 135/378 (35.71%), Postives = 198/378 (52.38%), Query Frame = 0
Query: 3 FISA-LIFLLRPST-KLPPSPPFSLPIIDHLYLLKRPVHQTLQKLSKKYG--HVFYLRLG 62
FIS L+F R S LPPSP LP+I HL+LLK P+H+T S+ G +F LRLG
Sbjct: 30 FISVKLLFGKRLSKFNLPPSPARPLPLIGHLHLLKLPLHRTFLSFSQSLGGAPIFCLRLG 89
Query: 63 SQLVVIVSSPSAVKECFTMNDIILANQPSSNIIKHLTYNNTIFSVSPYGDRWRELRRIST 122
++L V+VSS S +ECFT NDI+ AN+P + KH+ YN+T + +PYGD WR LRRI+T
Sbjct: 90 NRLTVVVSSYSIAEECFTKNDIVFANRPELILGKHIEYNSTTMTSAPYGDHWRNLRRIAT 149
Query: 123 IEIFSATRLNMFLETRRDEIKHLL-------------ALMGSMISELSSNVVMRMVMGKR 182
+EIF++ +LN FL R+DEI+ LL M ++ EL+ N V RMV GKR
Sbjct: 150 LEIFASHKLNGFLSVRKDEIRQLLLRLSKNSRHGFAKVEMRHLLFELTINNVFRMVAGKR 209
Query: 183 FYGEG--DLEQARKFRDIVKQVMQKCGSFDSRNFIPLLNWIDITHYEKR------RNDEF 242
FYGEG E A++ R ++ +++ G+ ++ +++P++ W T++EKR R D+F
Sbjct: 210 FYGEGTEQDEVAQQVRHLMDEIVTSAGAGNAADYLPIMRW--FTNFEKRVKNLAIRIDKF 269
Query: 243 LQGI-------------------------------------------------------- 284
LQ +
Sbjct: 270 LQSLVDEKRADKEKGTTMIDHLLSLQESQPDYYTDVTLKGIIIVMIIAGSETIAWTLEWA 329
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| W8JMU7 | 5.0e-58 | 36.77 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
| Q6WNQ8 | 1.2e-54 | 37.24 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
| Q6WNQ9 | 3.4e-54 | 35.25 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
| P93147 | 5.7e-54 | 35.48 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
| Q9FG65 | 1.0e-50 | 36.39 | Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1FUI0 | 5.7e-121 | 67.29 | cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448921 PE=3 SV=... | [more] |
| A0A6J1D5S6 | 4.5e-70 | 44.66 | isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE... | [more] |
| A0A7N2M2B9 | 7.7e-70 | 44.39 | Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1 | [more] |
| A0A6J1FSK8 | 1.0e-69 | 44.17 | cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448412 PE=3 SV=... | [more] |
| A0A0A0LTD0 | 1.3e-69 | 43.88 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G37370.1 | 2.0e-54 | 36.14 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
| AT4G37320.1 | 4.5e-54 | 34.23 | cytochrome P450, family 81, subfamily D, polypeptide 5 | [more] |
| AT4G37330.1 | 1.3e-53 | 34.76 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
| AT2G23190.1 | 6.5e-53 | 35.98 | cytochrome P450, family 81, subfamily D, polypeptide 7 | [more] |
| AT2G23220.1 | 1.1e-52 | 35.71 | cytochrome P450, family 81, subfamily D, polypeptide 6 | [more] |
