CmaCh20G009050 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G009050
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionInositol-tetrakisphosphate 1-kinase
LocationCma_Chr20: 4428883 .. 4429923 (+)
RNA-Seq ExpressionCmaCh20G009050
SyntenyCmaCh20G009050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTTCTTCTTCTTCTTCGTCGAATCGCCTTCGAGTAGGCTACGCTTTTCCTCCTAATAAAGAGCGCAATGTGATTCGCCCTTCTCTCGTTGATTACGCCAAGCTCCATGGTGTCGACCTGGTCCGTATCGACATCCAAACGCCGCTCCTCCACCAAGGTCCATTTCATTGCATTATTCACAAACTCTATGACGATGCTTGGGCGGAGCATCTGCAGGACTTCGCCTCGAAGAATCCGGACGTCGTCGTCGTGGATCGTCCGGACCTAATCGCTCAACTCTACGACAGAGTCTCCATGCTTGATGTGGTGAGCCAAGTGAAGGTTTCGGACAGCGGCGTGAGGATCGAGGTGCCGAAACAGATCGTTCTGAAACAGGAGGATGGAGAAATCGATTCCATTAGGGATTTGGATGTGAAATTTCCAGTGATTGCGAAACCGTTGGCGTCGGATGGAAGTGCGAAATCGCATGAAATGTCTCTGGTTTTCAATCGACGTGGATTAAAGGATTTGGAAACGCCCGTGCTTCTACAGGAGTTTGTGAACCACGGCGGAGTAATGTTCAAAGTATACGTCGCTGGAGAGGAATCCGTGTGCGTGAAACGGAAATCGCTGCCGGATGTGACAGAGACAGAGGAGGAACTAGAGAAGAAACCAGACGGCGCGATGAAATTTTCGCAAATATCTAGGGCAGAGGAGAAGAGCGAAAAATGTGACGGCGCTGAGAAGAAGGCGGCGGCGGAGAAGGAAGAAGAGATCGAAATGCCGCCGGAGAAGATAGTTAGGGAGGTATCGAATGGGCTGAAGGAAGCGATGGGGATTAGGCTGTTCAATTTCGACATGATTAGGGACGGGAATGGTACATACTATGTGATAGACATAAATTACTTACCAGGATTTGCAGTACTGCCGGAGTACGAGCAATTTCTGACGAAGTTCTTCAAGGAAGTTGCGGAGAAGAAGAAGAAGAAGGTGGAAGAGGATGATTCCGCCATTGAAGACGGTAAAGCAAAACCGCGGTGCTGCTGTTTCCAATGA

mRNA sequence

ATGGCTTCTTCTTCTTCTTCTTCGTCGAATCGCCTTCGAGTAGGCTACGCTTTTCCTCCTAATAAAGAGCGCAATGTGATTCGCCCTTCTCTCGTTGATTACGCCAAGCTCCATGGTGTCGACCTGGTCCGTATCGACATCCAAACGCCGCTCCTCCACCAAGGTCCATTTCATTGCATTATTCACAAACTCTATGACGATGCTTGGGCGGAGCATCTGCAGGACTTCGCCTCGAAGAATCCGGACGTCGTCGTCGTGGATCGTCCGGACCTAATCGCTCAACTCTACGACAGAGTCTCCATGCTTGATGTGGTGAGCCAAGTGAAGGTTTCGGACAGCGGCGTGAGGATCGAGGTGCCGAAACAGATCGTTCTGAAACAGGAGGATGGAGAAATCGATTCCATTAGGGATTTGGATGTGAAATTTCCAGTGATTGCGAAACCGTTGGCGTCGGATGGAAGTGCGAAATCGCATGAAATGTCTCTGGTTTTCAATCGACGTGGATTAAAGGATTTGGAAACGCCCGTGCTTCTACAGGAGTTTGTGAACCACGGCGGAGTAATGTTCAAAGTATACGTCGCTGGAGAGGAATCCGTGTGCGTGAAACGGAAATCGCTGCCGGATGTGACAGAGACAGAGGAGGAACTAGAGAAGAAACCAGACGGCGCGATGAAATTTTCGCAAATATCTAGGGCAGAGGAGAAGAGCGAAAAATGTGACGGCGCTGAGAAGAAGGCGGCGGCGGAGAAGGAAGAAGAGATCGAAATGCCGCCGGAGAAGATAGTTAGGGAGGTATCGAATGGGCTGAAGGAAGCGATGGGGATTAGGCTGTTCAATTTCGACATGATTAGGGACGGGAATGGTACATACTATGTGATAGACATAAATTACTTACCAGGATTTGCAGTACTGCCGGAGTACGAGCAATTTCTGACGAAGTTCTTCAAGGAAGTTGCGGAGAAGAAGAAGAAGAAGGTGGAAGAGGATGATTCCGCCATTGAAGACGGTAAAGCAAAACCGCGGTGCTGCTGTTTCCAATGA

Coding sequence (CDS)

ATGGCTTCTTCTTCTTCTTCTTCGTCGAATCGCCTTCGAGTAGGCTACGCTTTTCCTCCTAATAAAGAGCGCAATGTGATTCGCCCTTCTCTCGTTGATTACGCCAAGCTCCATGGTGTCGACCTGGTCCGTATCGACATCCAAACGCCGCTCCTCCACCAAGGTCCATTTCATTGCATTATTCACAAACTCTATGACGATGCTTGGGCGGAGCATCTGCAGGACTTCGCCTCGAAGAATCCGGACGTCGTCGTCGTGGATCGTCCGGACCTAATCGCTCAACTCTACGACAGAGTCTCCATGCTTGATGTGGTGAGCCAAGTGAAGGTTTCGGACAGCGGCGTGAGGATCGAGGTGCCGAAACAGATCGTTCTGAAACAGGAGGATGGAGAAATCGATTCCATTAGGGATTTGGATGTGAAATTTCCAGTGATTGCGAAACCGTTGGCGTCGGATGGAAGTGCGAAATCGCATGAAATGTCTCTGGTTTTCAATCGACGTGGATTAAAGGATTTGGAAACGCCCGTGCTTCTACAGGAGTTTGTGAACCACGGCGGAGTAATGTTCAAAGTATACGTCGCTGGAGAGGAATCCGTGTGCGTGAAACGGAAATCGCTGCCGGATGTGACAGAGACAGAGGAGGAACTAGAGAAGAAACCAGACGGCGCGATGAAATTTTCGCAAATATCTAGGGCAGAGGAGAAGAGCGAAAAATGTGACGGCGCTGAGAAGAAGGCGGCGGCGGAGAAGGAAGAAGAGATCGAAATGCCGCCGGAGAAGATAGTTAGGGAGGTATCGAATGGGCTGAAGGAAGCGATGGGGATTAGGCTGTTCAATTTCGACATGATTAGGGACGGGAATGGTACATACTATGTGATAGACATAAATTACTTACCAGGATTTGCAGTACTGCCGGAGTACGAGCAATTTCTGACGAAGTTCTTCAAGGAAGTTGCGGAGAAGAAGAAGAAGAAGGTGGAAGAGGATGATTCCGCCATTGAAGACGGTAAAGCAAAACCGCGGTGCTGCTGTTTCCAATGA

Protein sequence

MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPKQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFVNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDGAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVLPEYEQFLTKFFKEVAEKKKKKVEEDDSAIEDGKAKPRCCCFQ
Homology
BLAST of CmaCh20G009050 vs. ExPASy Swiss-Prot
Match: Q9SBA5 (Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.8e-70
Identity = 147/323 (45.51%), Postives = 215/323 (66.56%), Query Frame = 0

Query: 5   SSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIHKL 64
           S S   R  VGYA    K+ + I+PSL+++++  G+DLV++D    LL QG   CIIHKL
Sbjct: 2   SDSIQERYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKL 61

Query: 65  YDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVK--VSDSGVRIEVPKQ 124
           YD  W E+L +F  K P V V+D P+ I +L++RVSML+V++Q++  VSDS  R  VP+Q
Sbjct: 62  YDVYWKENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDS-ERFGVPEQ 121

Query: 125 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFV 184
           +V+             ++KFPVIAKPL +DGSAKSH+M L++++ G+K L+ P++LQEFV
Sbjct: 122 VVVMDSSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFV 181

Query: 185 NHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEK-CDG 244
           NHGGV+FKVYV G+   CVKR+SLPD++E +    K   G++ FSQIS    + +K  + 
Sbjct: 182 NHGGVIFKVYVVGDHVKCVKRRSLPDISEEKIGTSK---GSLPFSQISNLTAQEDKNIEY 241

Query: 245 AEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGN--GTYYVIDINYLP 304
            E ++     E++EMPP   + +++  ++E+MG+ LFNFD+IRD      Y +IDINY P
Sbjct: 242 GEDRSL----EKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFP 301

Query: 305 GFAVLPEYEQFLTKFFKEVAEKK 323
           G+A +P YE  LT+FF ++  KK
Sbjct: 302 GYAKMPSYEPVLTEFFWDMVTKK 316

BLAST of CmaCh20G009050 vs. ExPASy Swiss-Prot
Match: Q84Y01 (Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.1e-67
Identity = 145/322 (45.03%), Postives = 201/322 (62.42%), Query Frame = 0

Query: 3   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIH 62
           SS  +   R  +GYA  P K+++ I+PSLV  A   G+DLV +D   PL  QGPFH +IH
Sbjct: 10  SSGVTHPPRYVIGYALAPKKQQSFIQPSLVAQAASRGMDLVPVDASQPLAEQGPFHLLIH 69

Query: 63  KLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQV-KVSDSGVRIEVPK 122
           KLY D W   L  FA+++P V +VD P  I +L++R+SML VVS++   +D      +P 
Sbjct: 70  KLYGDDWRAQLVAFAARHPAVPIVDPPHAIDRLHNRISMLQVVSELDHAADQDSTFGIPS 129

Query: 123 QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEF 182
           Q+V+       D      ++FP+IAKPL +DG+AKSH+MSLV++R GL  L  P++LQEF
Sbjct: 130 QVVVYDAAALADFGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLGKLRPPLVLQEF 189

Query: 183 VNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDG 242
           VNHGGV+FKVYV G    CVKR+SLPDV+    E +    G++ FSQ+S    +      
Sbjct: 190 VNHGGVIFKVYVVGGHVTCVKRRSLPDVS---PEDDASAQGSVSFSQVSNLPTERT---- 249

Query: 243 AEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRD--GNGTYYVIDINYLP 302
           AE+    +  E+  +PP   + +++ GL+ A+G++LFNFDMIRD      Y VIDINY P
Sbjct: 250 AEEYYGEKSLEDAVVPPAAFINQIAGGLRRALGLQLFNFDMIRDVRAGDRYLVIDINYFP 309

Query: 303 GFAVLPEYEQFLTKFFKEVAEK 322
           G+A +P YE  LT FF E+  K
Sbjct: 310 GYAKMPGYETVLTDFFWEMVHK 324

BLAST of CmaCh20G009050 vs. ExPASy Swiss-Prot
Match: A0JJZ6 (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=ITPK5 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.0e-65
Identity = 141/316 (44.62%), Postives = 199/316 (62.97%), Query Frame = 0

Query: 11  RLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIHKLYDDAWA 70
           R  +GYA  P K+++ I+PSLV  A   G+DLV +D   PL  QGPFH +IHKLY + W 
Sbjct: 15  RYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEWR 74

Query: 71  EHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKV---SDSGVRIEVPKQIVLKQ 130
             L  F++ +P V VVD P  I +L++R+SML VVS++ V   +       +P Q+V+  
Sbjct: 75  GQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVYD 134

Query: 131 EDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFVNHGGV 190
                DS     ++FP+IAKPL +DG+AKSH+MSLV++R GL+ L  P++LQEFVNHGGV
Sbjct: 135 AAALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGGV 194

Query: 191 MFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDGAEKKAA 250
           +FKVYV G    CVKR+SLPDV+ ++   +   +G++ FSQ+S    +      A++   
Sbjct: 195 IFKVYVVGAHVTCVKRRSLPDVS-SDVLQDASAEGSLSFSQVSNLPNERT----AQEYYD 254

Query: 251 AEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNG--TYYVIDINYLPGFAVLP 310
             + E+  MPP   + +++  L+ A+G+ LFNFDMIRD      Y VIDINY PG+A +P
Sbjct: 255 DMRLEDAIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMP 314

Query: 311 EYEQFLTKFFKEVAEK 322
            YE  LT FF E+  K
Sbjct: 315 GYETVLTDFFWEMVHK 325

BLAST of CmaCh20G009050 vs. ExPASy Swiss-Prot
Match: Q7XBW0 (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=ITPK5 PE=2 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.0e-65
Identity = 141/316 (44.62%), Postives = 199/316 (62.97%), Query Frame = 0

Query: 11  RLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIHKLYDDAWA 70
           R  +GYA  P K+++ I+PSLV  A   G+DLV +D   PL  QGPFH +IHKLY + W 
Sbjct: 15  RYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEWR 74

Query: 71  EHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKV---SDSGVRIEVPKQIVLKQ 130
             L  F++ +P V VVD P  I +L++R+SML VVS++ V   +       +P Q+V+  
Sbjct: 75  GQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVYD 134

Query: 131 EDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFVNHGGV 190
                DS     ++FP+IAKPL +DG+AKSH+MSLV++R GL+ L  P++LQEFVNHGGV
Sbjct: 135 AAALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGGV 194

Query: 191 MFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDGAEKKAA 250
           +FKVYV G    CVKR+SLPDV+ ++   +   +G++ FSQ+S    +      A++   
Sbjct: 195 IFKVYVVGAHVTCVKRRSLPDVS-SDVLQDASAEGSLSFSQVSNLPNERT----AQEYYD 254

Query: 251 AEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNG--TYYVIDINYLPGFAVLP 310
             + E+  MPP   + +++  L+ A+G+ LFNFDMIRD      Y VIDINY PG+A +P
Sbjct: 255 DMRLEDAIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMP 314

Query: 311 EYEQFLTKFFKEVAEK 322
            YE  LT FF E+  K
Sbjct: 315 GYETVLTDFFWEMVHK 325

BLAST of CmaCh20G009050 vs. ExPASy Swiss-Prot
Match: O81893 (Inositol-tetrakisphosphate 1-kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ITPK2 PE=1 SV=3)

HSP 1 Score: 242.3 bits (617), Expect = 8.2e-63
Identity = 130/318 (40.88%), Postives = 198/318 (62.26%), Query Frame = 0

Query: 11  RLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIHKLYDDAWA 70
           +L VGYA    K+++ ++P L   A+  G+  V ID+  PL  QGPF  ++HKL    W 
Sbjct: 80  KLVVGYALTSKKKKSFLQPKLELLARRKGIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWE 139

Query: 71  EHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPKQ-IVLKQED 130
           E ++D+  K+P+V V+D P  I ++Y+R SML  ++ +K+SD    + VPKQ +VLK   
Sbjct: 140 EVIEDYQQKHPEVTVLDPPGSIQRIYNRQSMLQGMADLKLSDCSGSLFVPKQMVVLKDSA 199

Query: 131 GEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFVNHGGVMF 190
              D++ +  +KFP++AKPL  DG+AKSH++ L ++RR L +L+ P++LQEFVNHGGVMF
Sbjct: 200 ASADAVVEAGLKFPLVAKPLWIDGTAKSHQLYLAYDRRSLAELDPPLVLQEFVNHGGVMF 259

Query: 191 KVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDGAEKKAAAE 250
           KV+V G+    ++R SLP+V+  E   + K DG  +F ++S A   ++  D   + A   
Sbjct: 260 KVFVVGDVIKVMRRFSLPNVSNCE---KAKVDGVFQFPRVSSAAASADNADLDPRVA--- 319

Query: 251 KEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRD--GNGTYYVIDINYLPGFAVLPEY 310
                E+PP+  +  +   L+  +G+RLFN DMIR+      +YVIDINY PG+  LP+Y
Sbjct: 320 -----ELPPKPFLEALVKELRSLLGLRLFNIDMIREHGSKNVFYVIDINYFPGYGKLPDY 379

Query: 311 EQFLTKFFKEVAEKKKKK 326
           EQ    FF+ +A+ K KK
Sbjct: 380 EQVFVDFFQNLAQVKYKK 386

BLAST of CmaCh20G009050 vs. ExPASy TrEMBL
Match: A0A6J1JBH8 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111484264 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.1e-190
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCI 60
           MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCI
Sbjct: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCI 60

Query: 61  IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVP 120
           IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVP
Sbjct: 61  IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVP 120

Query: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQE 180
           KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQE
Sbjct: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQE 180

Query: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCD 240
           FVNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCD
Sbjct: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCD 240

Query: 241 GAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPG 300
           GAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPG
Sbjct: 241 GAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPG 300

Query: 301 FAVLPEYEQFLTKFFKEVAEKKKKKVEEDDSAIEDGKAKPRCCCFQ 347
           FAVLPEYEQFLTKFFKEVAEKKKKKVEEDDSAIEDGKAKPRCCCFQ
Sbjct: 301 FAVLPEYEQFLTKFFKEVAEKKKKKVEEDDSAIEDGKAKPRCCCFQ 346

BLAST of CmaCh20G009050 vs. ExPASy TrEMBL
Match: A0A6J1FTQ5 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC111448363 PE=3 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 6.5e-180
Identity = 331/347 (95.39%), Postives = 336/347 (96.83%), Query Frame = 0

Query: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCI 60
           MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRID+QTPLLHQGPFHCI
Sbjct: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI 60

Query: 61  IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVP 120
           IHKLYDDAWAEHLQDFA KNPDVVVVDRPDLIA+LYDRVSMLDVVSQVK SDSGVRIEVP
Sbjct: 61  IHKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP 120

Query: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQE 180
           KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDL+ PVLLQE
Sbjct: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE 180

Query: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCD 240
           FVNHGGVMFKVYVAGEESVCVKRKSLPDV ETEEELEKK DGAMKFSQISRAEEKSEKC+
Sbjct: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN 240

Query: 241 GAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPG 300
           GAEKK A    EEIEMPPEKIVREVSNGL+EAMGIRLFNFDMIRDGNGTYYVIDINYLPG
Sbjct: 241 GAEKKEA----EEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPG 300

Query: 301 FAVLPEYEQFLTKFFKEVAE-KKKKKVEEDDSAIEDGKAKPRCCCFQ 347
           FAVLPEYEQFLTKFFKEVAE KKKKKVEEDDSAIEDGKAKPRCCCFQ
Sbjct: 301 FAVLPEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ 343

BLAST of CmaCh20G009050 vs. ExPASy TrEMBL
Match: A0A5A7TY89 (Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold66G00690 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 1.1e-158
Identity = 294/345 (85.22%), Postives = 319/345 (92.46%), Query Frame = 0

Query: 3   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIH 62
           +S+S SSNRLRVGYAFPPNKERNVIRPSL+DYAK HGVDLVRID+QTPLLHQGPFHCIIH
Sbjct: 2   ASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKFHGVDLVRIDLQTPLLHQGPFHCIIH 61

Query: 63  KLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPKQ 122
           KLYDDAWAE+LQDFASKNPDVVVVDRPDLI++LY+RVSMLDVVSQVKVSDSGVRIEVPKQ
Sbjct: 62  KLYDDAWAENLQDFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSGVRIEVPKQ 121

Query: 123 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFV 182
           I++K+E+  IDSI +L++KFPVIAKP+ SDGSAKSHEMSLVFNRRGLK+L TPVLLQEFV
Sbjct: 122 ILVKKEEEAIDSIMNLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKELNTPVLLQEFV 181

Query: 183 NHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDGA 242
           NHGGVMFK+YVAG++SVCVKRKSLPDV  TEEELEKK +GAMKFSQISRAEEKSEKC+G 
Sbjct: 182 NHGGVMFKIYVAGDQSVCVKRKSLPDVEGTEEELEKKTEGAMKFSQISRAEEKSEKCNGE 241

Query: 243 EKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGFA 302
            KK    +E+EIEMPPEKIVREVS GLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGFA
Sbjct: 242 AKK----EEKEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGFA 301

Query: 303 VLPEYEQFLTKFFKEVAEKKKKKVEEDDSAIEDGKAKPRCC-CFQ 347
           VLPEYE FLTKFFKEV E   KKV+E  SAIED KAKPRCC CFQ
Sbjct: 302 VLPEYEPFLTKFFKEVTE---KKVDEVASAIEDDKAKPRCCSCFQ 339

BLAST of CmaCh20G009050 vs. ExPASy TrEMBL
Match: A0A1S3C9X3 (Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo OX=3656 GN=LOC103498455 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 1.1e-158
Identity = 294/345 (85.22%), Postives = 319/345 (92.46%), Query Frame = 0

Query: 3   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIH 62
           +S+S SSNRLRVGYAFPPNKERNVIRPSL+DYAK HGVDLVRID+QTPLLHQGPFHCIIH
Sbjct: 2   ASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKFHGVDLVRIDLQTPLLHQGPFHCIIH 61

Query: 63  KLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPKQ 122
           KLYDDAWAE+LQDFASKNPDVVVVDRPDLI++LY+RVSMLDVVSQVKVSDSGVRIEVPKQ
Sbjct: 62  KLYDDAWAENLQDFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSGVRIEVPKQ 121

Query: 123 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFV 182
           I++K+E+  IDSI +L++KFPVIAKP+ SDGSAKSHEMSLVFNRRGLK+L TPVLLQEFV
Sbjct: 122 ILVKKEEEAIDSIMNLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKELNTPVLLQEFV 181

Query: 183 NHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDGA 242
           NHGGVMFK+YVAG++SVCVKRKSLPDV  TEEELEKK +GAMKFSQISRAEEKSEKC+G 
Sbjct: 182 NHGGVMFKIYVAGDQSVCVKRKSLPDVEGTEEELEKKTEGAMKFSQISRAEEKSEKCNGE 241

Query: 243 EKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGFA 302
            KK    +E+EIEMPPEKIVREVS GLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGFA
Sbjct: 242 AKK----EEKEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGFA 301

Query: 303 VLPEYEQFLTKFFKEVAEKKKKKVEEDDSAIEDGKAKPRCC-CFQ 347
           VLPEYE FLTKFFKEV E   KKV+E  SAIED KAKPRCC CFQ
Sbjct: 302 VLPEYEPFLTKFFKEVTE---KKVDEVASAIEDDKAKPRCCSCFQ 339

BLAST of CmaCh20G009050 vs. ExPASy TrEMBL
Match: A0A6J1L0P8 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111499970 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.0e-157
Identity = 290/345 (84.06%), Postives = 318/345 (92.17%), Query Frame = 0

Query: 2   ASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCII 61
           +SSSSSSS+  RVGYAFPPNKERNVIRPSL+DYAKLHGVDLVRID+QTP+LHQGPFHCII
Sbjct: 7   SSSSSSSSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCII 66

Query: 62  HKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPK 121
           HK+YDDAW + LQDFASKNPDVVVVDRPD I +LY+RVSMLDVV+QVKVSDSGV+IEVPK
Sbjct: 67  HKMYDDAWVDTLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPK 126

Query: 122 QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEF 181
           Q+VL ++DG ID IRDL+VKFPVIAKPL S+GSAKSHEMSLV+NRRG KDLETPVLLQEF
Sbjct: 127 QVVLNRKDGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEF 186

Query: 182 VNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCD- 241
           VNHGGVMFKVYVAGE+SVCVKRKSLPDV ETEEELEKK DGAMKFSQISRAEEKSEKC+ 
Sbjct: 187 VNHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNC 246

Query: 242 GAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPG 301
           G +K+AAA  EEEIEMPPEK+V EVS GL+EAMGIRLFNFDMIRD NGTYYVIDINYLPG
Sbjct: 247 GGKKEAAA--EEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDRNGTYYVIDINYLPG 306

Query: 302 FAVLPEYEQFLTKFFKEVAEKKKKKVEEDDSAIEDGKAKPRCCCF 346
           FAVLP+YE FLTKFF EV +  KK VEE++SA+ED KAKPRCCCF
Sbjct: 307 FAVLPDYEAFLTKFFNEVMQ--KKVVEEENSAMEDEKAKPRCCCF 347

BLAST of CmaCh20G009050 vs. NCBI nr
Match: XP_022986561.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita maxima])

HSP 1 Score: 675.6 bits (1742), Expect = 2.2e-190
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCI 60
           MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCI
Sbjct: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCI 60

Query: 61  IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVP 120
           IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVP
Sbjct: 61  IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVP 120

Query: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQE 180
           KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQE
Sbjct: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQE 180

Query: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCD 240
           FVNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCD
Sbjct: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCD 240

Query: 241 GAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPG 300
           GAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPG
Sbjct: 241 GAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPG 300

Query: 301 FAVLPEYEQFLTKFFKEVAEKKKKKVEEDDSAIEDGKAKPRCCCFQ 347
           FAVLPEYEQFLTKFFKEVAEKKKKKVEEDDSAIEDGKAKPRCCCFQ
Sbjct: 301 FAVLPEYEQFLTKFFKEVAEKKKKKVEEDDSAIEDGKAKPRCCCFQ 346

BLAST of CmaCh20G009050 vs. NCBI nr
Match: XP_023512946.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 657.1 bits (1694), Expect = 8.2e-185
Identity = 337/346 (97.40%), Postives = 343/346 (99.13%), Query Frame = 0

Query: 2   ASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCII 61
           +SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRID+QTPLLHQGPFHCII
Sbjct: 4   SSSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCII 63

Query: 62  HKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPK 121
           HKLYDDAWAEHLQDFASKNPDVVVVDRPDLIA+LYDRVSMLDVVSQVK SDSGVRIEVPK
Sbjct: 64  HKLYDDAWAEHLQDFASKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPK 123

Query: 122 QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEF 181
           QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEF
Sbjct: 124 QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEF 183

Query: 182 VNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDG 241
           VNHGGVMFKVYVAGEESVCVKRKSLPDV ETEEELEKKPDGAMKFSQISRAEEKSEKC+G
Sbjct: 184 VNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKPDGAMKFSQISRAEEKSEKCNG 243

Query: 242 AEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGF 301
           AEKKAAAE+EEEIEMPPEKIVREVSNGL+EAMGIRLFNFDMIRDGNGTYYVIDINYLPGF
Sbjct: 244 AEKKAAAEEEEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGF 303

Query: 302 AVLPEYEQFLTKFFKEVAEK-KKKKVEEDDSAIEDGKAKPRCCCFQ 347
           AVLPEYEQFLTKFFKEVAEK KKKKVEEDDSAIEDGKAKPRCCCFQ
Sbjct: 304 AVLPEYEQFLTKFFKEVAEKEKKKKVEEDDSAIEDGKAKPRCCCFQ 349

BLAST of CmaCh20G009050 vs. NCBI nr
Match: XP_022943687.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita moschata])

HSP 1 Score: 639.8 bits (1649), Expect = 1.4e-179
Identity = 331/347 (95.39%), Postives = 336/347 (96.83%), Query Frame = 0

Query: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCI 60
           MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRID+QTPLLHQGPFHCI
Sbjct: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI 60

Query: 61  IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVP 120
           IHKLYDDAWAEHLQDFA KNPDVVVVDRPDLIA+LYDRVSMLDVVSQVK SDSGVRIEVP
Sbjct: 61  IHKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP 120

Query: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQE 180
           KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDL+ PVLLQE
Sbjct: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE 180

Query: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCD 240
           FVNHGGVMFKVYVAGEESVCVKRKSLPDV ETEEELEKK DGAMKFSQISRAEEKSEKC+
Sbjct: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN 240

Query: 241 GAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPG 300
           GAEKK A    EEIEMPPEKIVREVSNGL+EAMGIRLFNFDMIRDGNGTYYVIDINYLPG
Sbjct: 241 GAEKKEA----EEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPG 300

Query: 301 FAVLPEYEQFLTKFFKEVAE-KKKKKVEEDDSAIEDGKAKPRCCCFQ 347
           FAVLPEYEQFLTKFFKEVAE KKKKKVEEDDSAIEDGKAKPRCCCFQ
Sbjct: 301 FAVLPEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ 343

BLAST of CmaCh20G009050 vs. NCBI nr
Match: KAG6571172.1 (Inositol-tetrakisphosphate 1-kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 637.5 bits (1643), Expect = 6.7e-179
Identity = 330/346 (95.38%), Postives = 336/346 (97.11%), Query Frame = 0

Query: 2   ASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCII 61
           +SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRID+QTPLLHQGPFHCII
Sbjct: 3   SSSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCII 62

Query: 62  HKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPK 121
           HKLYDDAWAEHLQDFASKNPDVVVVDRPDLIA+LYDRVSMLDVVSQVK SDSGVRIEVPK
Sbjct: 63  HKLYDDAWAEHLQDFASKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKGSDSGVRIEVPK 122

Query: 122 QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEF 181
           QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDL+ PVLLQEF
Sbjct: 123 QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEF 182

Query: 182 VNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDG 241
           VNHGGVMFKVYVAGEESVCVKRKSLPDV ETEEELEKK DGAMKFSQISRAEEKSEKC+G
Sbjct: 183 VNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNG 242

Query: 242 AEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGF 301
           AEKK A    EEIEMPPEKIVREVSNGL+EAMGIRLFNFDMIRDGNGTYYVIDINYLPGF
Sbjct: 243 AEKKEA----EEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGF 302

Query: 302 AVLPEYEQFLTKFFKEVAE-KKKKKVEEDDSAIEDGKAKPRCCCFQ 347
           AVLPEYEQFLTKFFKEVAE KKKKKVEEDDSAIEDGKAKPRCCCFQ
Sbjct: 303 AVLPEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ 344

BLAST of CmaCh20G009050 vs. NCBI nr
Match: KAG7010978.1 (Inositol-tetrakisphosphate 1-kinase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 613.2 bits (1580), Expect = 1.4e-171
Identity = 320/336 (95.24%), Postives = 325/336 (96.73%), Query Frame = 0

Query: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCI 60
           MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRID+QTPLLHQGPFHCI
Sbjct: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI 60

Query: 61  IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVP 120
           IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIA+LYDRVSMLDVVSQVK SDSGVRIEVP
Sbjct: 61  IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP 120

Query: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQE 180
           KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDL+ PVLLQE
Sbjct: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE 180

Query: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCD 240
           FVNHGGVMFKVYVAGEESVCVKRKSLPDV ETEEELEKK DGAMKFSQISRAEEKSEKC+
Sbjct: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN 240

Query: 241 GAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPG 300
           GAEKK A    EEIEMPPEKIVREVSNGL+EAMGIRLFNFD IRDGNGTYYVIDINYLPG
Sbjct: 241 GAEKKEA----EEIEMPPEKIVREVSNGLREAMGIRLFNFDTIRDGNGTYYVIDINYLPG 300

Query: 301 FAVLPEYEQFLTKFFKEVAEKKKK-KVEEDDSAIED 336
           FAVLPEYEQFLTKFFKEVAEKKKK KVEEDDSAIED
Sbjct: 301 FAVLPEYEQFLTKFFKEVAEKKKKEKVEEDDSAIED 332

BLAST of CmaCh20G009050 vs. TAIR 10
Match: AT5G16760.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.3e-71
Identity = 147/323 (45.51%), Postives = 215/323 (66.56%), Query Frame = 0

Query: 5   SSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIHKL 64
           S S   R  VGYA    K+ + I+PSL+++++  G+DLV++D    LL QG   CIIHKL
Sbjct: 2   SDSIQERYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKL 61

Query: 65  YDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVK--VSDSGVRIEVPKQ 124
           YD  W E+L +F  K P V V+D P+ I +L++RVSML+V++Q++  VSDS  R  VP+Q
Sbjct: 62  YDVYWKENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDS-ERFGVPEQ 121

Query: 125 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFV 184
           +V+             ++KFPVIAKPL +DGSAKSH+M L++++ G+K L+ P++LQEFV
Sbjct: 122 VVVMDSSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFV 181

Query: 185 NHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEK-CDG 244
           NHGGV+FKVYV G+   CVKR+SLPD++E +    K   G++ FSQIS    + +K  + 
Sbjct: 182 NHGGVIFKVYVVGDHVKCVKRRSLPDISEEKIGTSK---GSLPFSQISNLTAQEDKNIEY 241

Query: 245 AEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGN--GTYYVIDINYLP 304
            E ++     E++EMPP   + +++  ++E+MG+ LFNFD+IRD      Y +IDINY P
Sbjct: 242 GEDRSL----EKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFP 301

Query: 305 GFAVLPEYEQFLTKFFKEVAEKK 323
           G+A +P YE  LT+FF ++  KK
Sbjct: 302 GYAKMPSYEPVLTEFFWDMVTKK 316

BLAST of CmaCh20G009050 vs. TAIR 10
Match: AT4G33770.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 242.3 bits (617), Expect = 5.9e-64
Identity = 130/318 (40.88%), Postives = 198/318 (62.26%), Query Frame = 0

Query: 11  RLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIHKLYDDAWA 70
           +L VGYA    K+++ ++P L   A+  G+  V ID+  PL  QGPF  ++HKL    W 
Sbjct: 80  KLVVGYALTSKKKKSFLQPKLELLARRKGIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWE 139

Query: 71  EHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPKQ-IVLKQED 130
           E ++D+  K+P+V V+D P  I ++Y+R SML  ++ +K+SD    + VPKQ +VLK   
Sbjct: 140 EVIEDYQQKHPEVTVLDPPGSIQRIYNRQSMLQGMADLKLSDCSGSLFVPKQMVVLKDSA 199

Query: 131 GEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFVNHGGVMF 190
              D++ +  +KFP++AKPL  DG+AKSH++ L ++RR L +L+ P++LQEFVNHGGVMF
Sbjct: 200 ASADAVVEAGLKFPLVAKPLWIDGTAKSHQLYLAYDRRSLAELDPPLVLQEFVNHGGVMF 259

Query: 191 KVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDGAEKKAAAE 250
           KV+V G+    ++R SLP+V+  E   + K DG  +F ++S A   ++  D   + A   
Sbjct: 260 KVFVVGDVIKVMRRFSLPNVSNCE---KAKVDGVFQFPRVSSAAASADNADLDPRVA--- 319

Query: 251 KEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRD--GNGTYYVIDINYLPGFAVLPEY 310
                E+PP+  +  +   L+  +G+RLFN DMIR+      +YVIDINY PG+  LP+Y
Sbjct: 320 -----ELPPKPFLEALVKELRSLLGLRLFNIDMIREHGSKNVFYVIDINYFPGYGKLPDY 379

Query: 311 EQFLTKFFKEVAEKKKKK 326
           EQ    FF+ +A+ K KK
Sbjct: 380 EQVFVDFFQNLAQVKYKK 386

BLAST of CmaCh20G009050 vs. TAIR 10
Match: AT4G08170.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 232.3 bits (591), Expect = 6.1e-61
Identity = 130/316 (41.14%), Postives = 191/316 (60.44%), Query Frame = 0

Query: 14  VGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCIIHKLYDDAWAEHL 73
           VGYA    K ++ ++P L   A+  G+  V ID   PL  QGPF  ++HK     W   L
Sbjct: 43  VGYALTSKKIKSFLQPKLEGLARNKGILFVAIDQNKPLSEQGPFDIVLHKQIGKEWRRIL 102

Query: 74  QDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPKQIVLKQEDGEI- 133
           ++F   +PDV V+D PD I  L +R SML  V+ + +SDS  R+ VPKQ+V+K++   I 
Sbjct: 103 EEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVIKKDASSIP 162

Query: 134 DSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFVNHGGVMFKVY 193
           +++ +  ++ P++AKPL +DGSAKSHE+SL +++  L  LE P++LQEFVNHGGV+FKVY
Sbjct: 163 EAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNHGGVLFKVY 222

Query: 194 VAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDGAEKKAAAEKEE 253
           + GE    V+R SLPDV+  E     K  G  +F ++S A   ++  D     A      
Sbjct: 223 IVGEAIRVVRRFSLPDVSRRE---LPKSAGVFRFPRVSCAAASADDADLDPSIA------ 282

Query: 254 EIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGT---YYVIDINYLPGFAVLPEYEQ 313
             E+PP  ++  ++  L+  +G+RLFN D+IR+ +GT   +YVIDINY PG+  +PEYE 
Sbjct: 283 --ELPPRPLLERLAKELRRGLGLRLFNLDIIRE-HGTRDRFYVIDINYFPGYGKMPEYEH 342

Query: 314 FLTKFFKEVAEKKKKK 326
             T F   V + + KK
Sbjct: 343 VFTDFLLSVVQSQCKK 346

BLAST of CmaCh20G009050 vs. TAIR 10
Match: AT4G33770.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 226.1 bits (575), Expect = 4.3e-59
Identity = 121/290 (41.72%), Postives = 182/290 (62.76%), Query Frame = 0

Query: 39  GVDLVRIDIQTPLLHQGPFHCIIHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDR 98
           G+  V ID+  PL  QGPF  ++HKL    W E ++D+  K+P+V V+D P  I ++Y+R
Sbjct: 15  GIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWEEVIEDYQQKHPEVTVLDPPGSIQRIYNR 74

Query: 99  VSMLDVVSQVKVSDSGVRIEVPKQ-IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKS 158
            SML  ++ +K+SD    + VPKQ +VLK      D++ +  +KFP++AKPL  DG+AKS
Sbjct: 75  QSMLQGMADLKLSDCSGSLFVPKQMVVLKDSAASADAVVEAGLKFPLVAKPLWIDGTAKS 134

Query: 159 HEMSLVFNRRGLKDLETPVLLQEFVNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELE 218
           H++ L ++RR L +L+ P++LQEFVNHGGVMFKV+V G+    ++R SLP+V+  E   +
Sbjct: 135 HQLYLAYDRRSLAELDPPLVLQEFVNHGGVMFKVFVVGDVIKVMRRFSLPNVSNCE---K 194

Query: 219 KKPDGAMKFSQISRAEEKSEKCDGAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRL 278
            K DG  +F ++S A   ++  D   + A        E+PP+  +  +   L+  +G+RL
Sbjct: 195 AKVDGVFQFPRVSSAAASADNADLDPRVA--------ELPPKPFLEALVKELRSLLGLRL 254

Query: 279 FNFDMIRD--GNGTYYVIDINYLPGFAVLPEYEQFLTKFFKEVAEKKKKK 326
           FN DMIR+      +YVIDINY PG+  LP+YEQ    FF+ +A+ K KK
Sbjct: 255 FNIDMIREHGSKNVFYVIDINYFPGYGKLPDYEQVFVDFFQNLAQVKYKK 293

BLAST of CmaCh20G009050 vs. TAIR 10
Match: AT4G08170.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 195.3 bits (495), Expect = 8.2e-50
Identity = 110/261 (42.15%), Postives = 163/261 (62.45%), Query Frame = 0

Query: 69  WAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVPKQIVLKQE 128
           W   L++F   +PDV V+D PD I  L +R SML  V+ + +SDS  R+ VPKQ+V+K++
Sbjct: 10  WRRILEEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVIKKD 69

Query: 129 DGEI-DSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEFVNHGGV 188
              I +++ +  ++ P++AKPL +DGSAKSHE+SL +++  L  LE P++LQEFVNHGGV
Sbjct: 70  ASSIPEAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNHGGV 129

Query: 189 MFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCDGAEKKAA 248
           +FKVY+ GE    V+R SLPDV+  E     K  G  +F ++S A   ++  D     A 
Sbjct: 130 LFKVYIVGEAIRVVRRFSLPDVSRRE---LPKSAGVFRFPRVSCAAASADDADLDPSIA- 189

Query: 249 AEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGT---YYVIDINYLPGFAVL 308
                  E+PP  ++  ++  L+  +G+RLFN D+IR+ +GT   +YVIDINY PG+  +
Sbjct: 190 -------ELPPRPLLERLAKELRRGLGLRLFNLDIIRE-HGTRDRFYVIDINYFPGYGKM 249

Query: 309 PEYEQFLTKFFKEVAEKKKKK 326
           PEYE   T F   V + + KK
Sbjct: 250 PEYEHVFTDFLLSVVQSQCKK 258

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SBA51.8e-7045.51Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 P... [more]
Q84Y011.1e-6745.03Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1[more]
A0JJZ61.0e-6544.62Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q7XBW01.0e-6544.62Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
O818938.2e-6340.88Inositol-tetrakisphosphate 1-kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ITPK2 P... [more]
Match NameE-valueIdentityDescription
A0A6J1JBH81.1e-190100.00Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111484264 ... [more]
A0A6J1FTQ56.5e-18095.39Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC11144836... [more]
A0A5A7TY891.1e-15885.22Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3C9X31.1e-15885.22Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo OX=3656 GN=LOC103498455 PE=3... [more]
A0A6J1L0P82.0e-15784.06Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111499970 ... [more]
Match NameE-valueIdentityDescription
XP_022986561.12.2e-190100.00inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita maxima][more]
XP_023512946.18.2e-18597.40inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022943687.11.4e-17995.39inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita moschata][more]
KAG6571172.16.7e-17995.38Inositol-tetrakisphosphate 1-kinase 1, partial [Cucurbita argyrosperma subsp. so... [more]
KAG7010978.11.4e-17195.24Inositol-tetrakisphosphate 1-kinase 1, partial [Cucurbita argyrosperma subsp. ar... [more]
Match NameE-valueIdentityDescription
AT5G16760.11.3e-7145.51Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G33770.15.9e-6440.88Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G08170.26.1e-6141.14Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G33770.24.3e-5941.72Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G08170.18.2e-5042.15Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 232..252
NoneNo IPR availableGENE3D3.30.1490.220coord: 122..220
e-value: 2.4E-30
score: 106.2
NoneNo IPR availableGENE3D3.30.470.20coord: 221..302
e-value: 7.5E-15
score: 57.4
NoneNo IPR availableGENE3D3.40.50.11370coord: 2..114
e-value: 7.7E-23
score: 82.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 229..253
NoneNo IPR availablePANTHERPTHR14217:SF24INOSITOL-TETRAKISPHOSPHATE 1-KINASE 1coord: 7..324
NoneNo IPR availableSUPERFAMILY56059Glutathione synthetase ATP-binding domain-likecoord: 89..311
IPR008656Inositol-tetrakisphosphate 1-kinasePIRSFPIRSF038186ITPKcoord: 1..331
e-value: 4.4E-107
score: 355.8
IPR008656Inositol-tetrakisphosphate 1-kinasePANTHERPTHR14217INOSITOL-TETRAKISPHOSPHATE 1-KINASEcoord: 7..324
IPR041429Inositol-tetrakisphosphate 1-kinase, N-terminalPFAMPF17927Ins134_P3_kin_Ncoord: 13..92
e-value: 1.8E-23
score: 82.7
IPR040464Inositol 1,3,4-trisphosphate 5/6-kinase, ATP-grasp domainPFAMPF05770Ins134_P3_kincoord: 115..316
e-value: 2.7E-66
score: 222.9
IPR011761ATP-grasp foldPROSITEPS50975ATP_GRASPcoord: 109..327
score: 15.284159

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G009050.1CmaCh20G009050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0052746 inositol phosphorylation
biological_process GO:0032957 inositol trisphosphate metabolic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0052726 inositol-1,3,4-trisphosphate 5-kinase activity
molecular_function GO:0052725 inositol-1,3,4-trisphosphate 6-kinase activity
molecular_function GO:0047325 inositol tetrakisphosphate 1-kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0046872 metal ion binding