CmaCh20G008440 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G008440
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionisoflavone reductase-like protein
LocationCma_Chr20: 4043610 .. 4045636 (-)
RNA-Seq ExpressionCmaCh20G008440
SyntenyCmaCh20G008440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAGCAATATTTCTGTACGACGAAAAAGGAACTCTCCCTGTTTCTTCTTCTTTCTTTTTGCAGCTCGCCGGTAGTTCTCCGATCAGGTTATTCTTCGCCGGAAACCTTACTTCGATGGCTCAGAATCTCAAGGTTCTAATAATTGGTGGAACTGGTTACATCGGAAAATTCATTGTACAAGCTAGCGCCAAAGCCGGTCATCCTACCTATGCATTGATCCGCAAGTCTTCGCTCGGAAGCTCTGCCAAGTCTCAAATCATCAATCATTTCAAAAGCCTAGGGGTCAATTTTCTCTTCGTAAGTACTCTGTCCTCTATTTCTCTGGATTTTGTTGTTTCATTGCTCAGAATTTTTGTGGAATCCTTTGCTGAATAATGTGCCTGATTTTCTTGCTTTACTATTTTGTTAGCGAATGAGTAAACTGAGTTTGATTTGATTAAATCGAAGTGATTACTTGTAATGCAGCATATACTGGTGGTTCTTTTGAGTTGTTCTTATTGTAAATTCTGTGGAATCAAAATGAATTTTCGGAGTGGAAATGGTATTGTTTAATTTCGCTAAAACGCTGATATGATTGGGGCAGGGCGATCTCTTCGACAATGAGAGTTTGGTTAAGGCGATAAAGCAAGTTGATGTGGTGATATCCACCCTCGGTGGTCATATGGTTCCCCATCAACATAAGATCCTATCTGCTATCAAACAAGCTGGCAATGTCAAGGTAACCTCATTTCCCTGCCAATCTTTATCTACATTTTGCCTACAACTATGCCATTGATTGTGATTGCTTTGTTGCAATATGCATTCTTCTATAGAGTTTGTGTGTTTTGGAAATTTTGAGCATTGGAATTTTTGTGGTACAGAGATTCTTTCCTACGGAGTTCGGCAACGATGCCGATCACATCGACGCGGTGGAGCCAGCAAAATCAATGTACACTGCAAAGGCTGAATTTCGTAGGGTTGTTGAGGCTGAGGGAATCCCACACACATTCGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCATCCGTCCCACCTAGAGACAAAGTCGTAATCTTAGGGGATGGAACTCCCAAAGGTACAAATATGTTAACCGCATCCTCTCAAGTTCTTATTGAACCTTTTGTTTTGTTGGATGGATTGTTGTGCACTACTATTATCTCATTTTCTGGAGTTGAAATGGCTTCCAACGTAGTAACCCCCCTGGATATTCAATAGCTATTGATGCGTTTCATTGGTTTTTCATTCATCAAAAACTTATGCATAACATAGACACATGTTGTATATCATTGGCTACAATAAGAGTTTGGTTTCAATAGCCTCCCACACTGTTCATTCTGCACCTGTTTTGTGAAGTAGTGATTTACAACAAGGAAGAAGCTCTTCTAATAAACAAAACTTGTACACACACACACACACGTTATAAATAATTGACTACAACATGAAGCATCATTCGTAAGCAGTTTGATGAAGGGAGATATCGAGTTTGTTTCGTCATTCACCCTATTCATTATGTGTCTGTTTTGTTTGGTGAAGTGATTTACAACAAGGAAGAAGACGTTGGGACGTACACCATCCGAGCAATAGATGATCCAAGAACCCTCAACAAGATCATGTACATTAGACCCGCAGCCAACATCTACTCGACCAACGATCTGGTCTCGCTGTGGGAAAGAAAAATTGGCAAGACTCTTAGAAGGGTCTATGTCCCAGAGGAGGAGGTGTTGAAGAACATCCAAGGTGGGTTTTACCAAACAACAACTTTTCAATTCATAATCTGTCATGCTTCTAAAACACAAACTGATTAACACATTCGTTTTTGTTAATCATCTCTTGCAGAGGCCTCATATCCACTTAATATAGAATTGGCACTCTGTCACACTGCACAGGTGAGGGGATCTCAAACTAACTTTGATATTGAGCCATCTTTTGGAGTGGAAGCTTCAGCGCTGTACCCAGATGTTCGATATACAACTGTGGAAGAATACCTCGACCAGTTTGTCTAA

mRNA sequence

CAAAGCAATATTTCTGTACGACGAAAAAGGAACTCTCCCTGTTTCTTCTTCTTTCTTTTTGCAGCTCGCCGGTAGTTCTCCGATCAGGTTATTCTTCGCCGGAAACCTTACTTCGATGGCTCAGAATCTCAAGGTTCTAATAATTGGTGGAACTGGTTACATCGGAAAATTCATTGTACAAGCTAGCGCCAAAGCCGGTCATCCTACCTATGCATTGATCCGCAAGTCTTCGCTCGGAAGCTCTGCCAAGTCTCAAATCATCAATCATTTCAAAAGCCTAGGGGTCAATTTTCTCTTCGGCGATCTCTTCGACAATGAGAGTTTGGTTAAGGCGATAAAGCAAGTTGATGTGGTGATATCCACCCTCGGTGGTCATATGGTTCCCCATCAACATAAGATCCTATCTGCTATCAAACAAGCTGGCAATGTCAAGAGATTCTTTCCTACGGAGTTCGGCAACGATGCCGATCACATCGACGCGGTGGAGCCAGCAAAATCAATGTACACTGCAAAGGCTGAATTTCGTAGGGTTGTTGAGGCTGAGGGAATCCCACACACATTCGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCATCCGTCCCACCTAGAGACAAAGTCGTAATCTTAGGGGATGGAACTCCCAAAGTGATTTACAACAAGGAAGAAGACGTTGGGACGTACACCATCCGAGCAATAGATGATCCAAGAACCCTCAACAAGATCATGTACATTAGACCCGCAGCCAACATCTACTCGACCAACGATCTGGTCTCGCTGTGGGAAAGAAAAATTGGCAAGACTCTTAGAAGGGTCTATGTCCCAGAGGAGGAGGTGTTGAAGAACATCCAAGAGGCCTCATATCCACTTAATATAGAATTGGCACTCTGTCACACTGCACAGGTGAGGGGATCTCAAACTAACTTTGATATTGAGCCATCTTTTGGAGTGGAAGCTTCAGCGCTGTACCCAGATGTTCGATATACAACTGTGGAAGAATACCTCGACCAGTTTGTCTAA

Coding sequence (CDS)

ATGGCTCAGAATCTCAAGGTTCTAATAATTGGTGGAACTGGTTACATCGGAAAATTCATTGTACAAGCTAGCGCCAAAGCCGGTCATCCTACCTATGCATTGATCCGCAAGTCTTCGCTCGGAAGCTCTGCCAAGTCTCAAATCATCAATCATTTCAAAAGCCTAGGGGTCAATTTTCTCTTCGGCGATCTCTTCGACAATGAGAGTTTGGTTAAGGCGATAAAGCAAGTTGATGTGGTGATATCCACCCTCGGTGGTCATATGGTTCCCCATCAACATAAGATCCTATCTGCTATCAAACAAGCTGGCAATGTCAAGAGATTCTTTCCTACGGAGTTCGGCAACGATGCCGATCACATCGACGCGGTGGAGCCAGCAAAATCAATGTACACTGCAAAGGCTGAATTTCGTAGGGTTGTTGAGGCTGAGGGAATCCCACACACATTCGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCATCCGTCCCACCTAGAGACAAAGTCGTAATCTTAGGGGATGGAACTCCCAAAGTGATTTACAACAAGGAAGAAGACGTTGGGACGTACACCATCCGAGCAATAGATGATCCAAGAACCCTCAACAAGATCATGTACATTAGACCCGCAGCCAACATCTACTCGACCAACGATCTGGTCTCGCTGTGGGAAAGAAAAATTGGCAAGACTCTTAGAAGGGTCTATGTCCCAGAGGAGGAGGTGTTGAAGAACATCCAAGAGGCCTCATATCCACTTAATATAGAATTGGCACTCTGTCACACTGCACAGGTGAGGGGATCTCAAACTAACTTTGATATTGAGCCATCTTTTGGAGTGGAAGCTTCAGCGCTGTACCCAGATGTTCGATATACAACTGTGGAAGAATACCTCGACCAGTTTGTCTAA

Protein sequence

MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFLFGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHIDAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRRVYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTVEEYLDQFV
Homology
BLAST of CmaCh20G008440 vs. ExPASy Swiss-Prot
Match: O81355 (Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC5 PE=1 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 4.0e-125
Identity = 214/308 (69.48%), Postives = 260/308 (84.42%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           MA   ++L IGGTGYIGKFIV+ASAKAG+PTY L+R++SL   AKS++I +FK+LGVNF+
Sbjct: 1   MASKSQILFIGGTGYIGKFIVEASAKAGYPTYVLVREASLSDPAKSKVIENFKALGVNFV 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
            GDL+D+ESLVKAIKQVDVVIST+G   +  Q KI++AIK+AGNVKRFFP+EFGND D  
Sbjct: 61  LGDLYDHESLVKAIKQVDVVISTVGHGQLADQGKIIAAIKEAGNVKRFFPSEFGNDVDRS 120

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
            AVEPAKS +  KA+ RR VEAEGIP+T+V  NFF GYFL  L+QP AS  PRDKVVILG
Sbjct: 121 HAVEPAKSAFETKAKIRRAVEAEGIPYTYVSSNFFAGYFLPTLNQPGASSAPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DG PK I+NKE+D+GTYTIRA+DDPRTLNK++YIRP AN  S N+LVSLWE+KIGKTL R
Sbjct: 181 DGNPKAIFNKEDDIGTYTIRAVDDPRTLNKVLYIRPPANTISFNELVSLWEKKIGKTLER 240

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           +YVPEE++LKNIQEA+ PLN+ L++ H   V+G  TNF+IEPSFGVEA+ALYPDV+YTTV
Sbjct: 241 IYVPEEQLLKNIQEAAVPLNVILSISHAVFVKGDHTNFEIEPSFGVEATALYPDVKYTTV 300

Query: 301 EEYLDQFV 309
           +EYL+QFV
Sbjct: 301 DEYLNQFV 308

BLAST of CmaCh20G008440 vs. ExPASy Swiss-Prot
Match: B9HRL7 (Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=PCBER PE=1 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 1.1e-124
Identity = 210/303 (69.31%), Postives = 258/303 (85.15%), Query Frame = 0

Query: 6   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFLFGDLF 65
           K+L IGGTGYIGKFIV+ASAKAGHPT+ L+R+S+L + AKS +I +FK+LGVNFL GDLF
Sbjct: 4   KILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSNPAKSVVIYNFKNLGVNFLIGDLF 63

Query: 66  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHIDAVEP 125
           D+ESLVKAIKQVDVVIST+G   +  Q +I++AIK+AGNVKRFFP+EFGND D ++AVEP
Sbjct: 64  DHESLVKAIKQVDVVISTVGHAQLVEQDRIIAAIKEAGNVKRFFPSEFGNDVDRVNAVEP 123

Query: 126 AKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 185
           AKS +  KA  RR +EAEGIP+T+V  NFF GYFL + +QP A+ PPRDKVVILGDG PK
Sbjct: 124 AKSAFATKANVRRAIEAEGIPYTYVSSNFFSGYFLLSFNQPGATAPPRDKVVILGDGNPK 183

Query: 186 VIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRRVYVPE 245
            ++NKE+D+ TYTI+A+DDPRTLNKI+YI+P AN  S NDLVSLWE+KIGKTL R+YVPE
Sbjct: 184 AVFNKEDDIATYTIKAVDDPRTLNKILYIKPPANTISFNDLVSLWEKKIGKTLERIYVPE 243

Query: 246 EEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTVEEYLD 305
           E++LKNIQEAS P+N+ L++ H+  V+G  TNF+IEPSFGVEAS LYPDV+YTTV+EYL 
Sbjct: 244 EQLLKNIQEASVPVNVVLSIGHSVFVKGDHTNFEIEPSFGVEASELYPDVKYTTVDEYLK 303

Query: 306 QFV 309
           QFV
Sbjct: 304 QFV 306

BLAST of CmaCh20G008440 vs. ExPASy Swiss-Prot
Match: B2WSN0 (Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 3.5e-121
Identity = 203/309 (65.70%), Postives = 258/309 (83.50%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           M    K+LIIGGTGYIGKFIV+AS K GHPT+AL+R++++    K +++  F++LGV+ L
Sbjct: 1   MGSKSKILIIGGTGYIGKFIVEASVKEGHPTFALVRETTVSDPVKGKLVEKFQNLGVSLL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
           +GDL+D++SLVKAIKQVDVVIST+G   +  Q KI++AIK+AGNVKRFFP+EFGND DH+
Sbjct: 61  YGDLYDHDSLVKAIKQVDVVISTVGFMQIADQTKIIAAIKEAGNVKRFFPSEFGNDVDHV 120

Query: 121 DAVEPAKSM-YTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVIL 180
           +AVEPAKS+ +  KA  RR VEAEGIP+T+V  N F+GYFL  L QP A+ PPRDKV+I 
Sbjct: 121 NAVEPAKSVAFAVKANIRRAVEAEGIPYTYVASNCFNGYFLPTLVQPGATTPPRDKVIIP 180

Query: 181 GDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLR 240
           GDG PK I+NKEED+GTYTI+A+DDPRTLNKI+Y+RP+ NIYS N+LV+LWE+KIGKTL 
Sbjct: 181 GDGNPKAIFNKEEDIGTYTIKAVDDPRTLNKILYLRPSNNIYSFNELVALWEKKIGKTLE 240

Query: 241 RVYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTT 300
           ++YVPEE++LK+IQEA  P+NI L + H+  V+G  TNF+IEPSFGVEAS LYP+V+YTT
Sbjct: 241 KIYVPEEQILKDIQEAPIPINIFLGINHSVFVKGDHTNFEIEPSFGVEASELYPEVKYTT 300

Query: 301 VEEYLDQFV 309
           VEEYLDQFV
Sbjct: 301 VEEYLDQFV 309

BLAST of CmaCh20G008440 vs. ExPASy Swiss-Prot
Match: Q9T030 (Phenylcoumaran benzylic ether reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PCBER1 PE=1 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 2.2e-120
Identity = 203/308 (65.91%), Postives = 250/308 (81.17%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           M    K+L IGGTGYIGK+IV+ASA++GHPT  L+R S+L S ++S  I +FK+LGV FL
Sbjct: 1   MTSKSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
            GDL D+ SLV +IKQ DVVIST+G  ++ HQ+KI+SAIK+AGNVKRFFP+EFGND D +
Sbjct: 61  LGDLDDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRV 120

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
             VEPAKS Y  KA+ RR +EAEGIP+T+V CNFF GYFL  L+QP A+  PRDKV++LG
Sbjct: 121 FTVEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DG PK ++NKEED+GTYTI A+DDPRTLNKI+YIRP  N YS NDLVSLWE KIGKTL R
Sbjct: 181 DGNPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLER 240

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           +YVPEE++LK I E+S PLN+ L+LCH   V+G  T+F+IEPSFGVEAS LYPDV+YTTV
Sbjct: 241 IYVPEEQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTV 300

Query: 301 EEYLDQFV 309
           +E L+Q+V
Sbjct: 301 DEILNQYV 308

BLAST of CmaCh20G008440 vs. ExPASy Swiss-Prot
Match: Q9FUW6 (Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6 PE=1 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.1e-118
Identity = 199/308 (64.61%), Postives = 252/308 (81.82%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           MA   K+LIIGGTGYIGKFIV+ASAK+GHPT+AL+R+S++    K +++  FK LGV  L
Sbjct: 1   MAHKSKILIIGGTGYIGKFIVEASAKSGHPTFALVRESTVSDPVKGKLVEKFKGLGVTLL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
            GDL+D+ESLVKA KQVDVVIST+G   +  Q KI++AIK+AGN+KRFFP+EFGND D +
Sbjct: 61  HGDLYDHESLVKAFKQVDVVISTVGHLQLADQVKIIAAIKEAGNIKRFFPSEFGNDVDRV 120

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
            AVEPAK+ +  KAE RR  EAEGIP+T+V  NFF GYFL  L+QP  + PPR+KVVI G
Sbjct: 121 HAVEPAKTAFATKAEIRRKTEAEGIPYTYVSSNFFAGYFLPTLAQPGLTSPPREKVVIFG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DG  + ++NKE+D+GTYTIRA+DDPRTLNKI+YI+PA NIYS N++V+LWE+KIGKTL +
Sbjct: 181 DGNARAVFNKEDDIGTYTIRAVDDPRTLNKIVYIKPAKNIYSFNEIVALWEKKIGKTLEK 240

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           +YVPEE++LK+IQE+  P+N+ LA+ H+  V+G  TNF+IE SFGVEAS LYPDV+YTTV
Sbjct: 241 IYVPEEKLLKDIQESPIPINVILAINHSVFVKGDHTNFEIEASFGVEASELYPDVKYTTV 300

Query: 301 EEYLDQFV 309
           EEYL QFV
Sbjct: 301 EEYLQQFV 308

BLAST of CmaCh20G008440 vs. ExPASy TrEMBL
Match: A0A6J1JEF2 (isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111484277 PE=4 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 6.2e-174
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL
Sbjct: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
           DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG
Sbjct: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQFV 309
           EEYLDQFV
Sbjct: 301 EEYLDQFV 308

BLAST of CmaCh20G008440 vs. ExPASy TrEMBL
Match: A0A6J1FXY6 (isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111448291 PE=4 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.2e-172
Identity = 306/308 (99.35%), Postives = 306/308 (99.35%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGV FL
Sbjct: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVKFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
           DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG
Sbjct: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRP ANIYSTNDLVSLWERKIGKTLRR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPPANIYSTNDLVSLWERKIGKTLRR 240

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQFV 309
           EEYLDQFV
Sbjct: 301 EEYLDQFV 308

BLAST of CmaCh20G008440 vs. ExPASy TrEMBL
Match: A0A1S3CE33 (isoflavone reductase-like protein OS=Cucumis melo OX=3656 GN=LOC103499680 PE=4 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 3.8e-163
Identity = 288/308 (93.51%), Postives = 300/308 (97.40%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           MAQ  KVLIIGGTGYIGKFIVQASAKAGHPTYALIR+SSL S AK++I+NHFKSLGVNFL
Sbjct: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFP+EFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
           DAVEPAKSMY+AKAEFRR VEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG
Sbjct: 121 DAVEPAKSMYSAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMY+RP ANIYSTNDLVSLWERKIGK+L+R
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           +YVPEEEVLK I+E SYPLNIELALCHTAQV+GSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQFV 309
           EEYLDQFV
Sbjct: 301 EEYLDQFV 306

BLAST of CmaCh20G008440 vs. ExPASy TrEMBL
Match: A0A5A7TR10 (Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G00570 PE=4 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 4.2e-162
Identity = 286/308 (92.86%), Postives = 299/308 (97.08%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           MAQ  KVLIIGGTGYIGKFIVQASAKAGHPTYALIR+SSL S AK++I+NHFKSLGVNFL
Sbjct: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFP+EFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
           DAVEPAKSMY+AKAEFRR VEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG
Sbjct: 121 DAVEPAKSMYSAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMY+RP ANIYSTNDLVSLWERKIGK+L+R
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           +YVPEEEVLK I+E SYPLNIELALCHTAQV+GSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQFV 309
           EEYLDQ +
Sbjct: 301 EEYLDQAI 306

BLAST of CmaCh20G008440 vs. ExPASy TrEMBL
Match: A0A5D3DYL6 (Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00390 PE=4 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 4.2e-162
Identity = 286/306 (93.46%), Postives = 298/306 (97.39%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           MAQ  KVLIIGGTGYIGKFIVQASAKAGHPTYALIR+SSL S AK++I+NHFKSLGVNFL
Sbjct: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFP+EFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
           DAVEPAKSMY+AKAEFRR VEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG
Sbjct: 121 DAVEPAKSMYSAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMY+RP ANIYSTNDLVSLWERKIGK+L+R
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           +YVPEEEVLK I+E SYPLNIELALCHTAQV+GSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQ 307
           EEYLDQ
Sbjct: 301 EEYLDQ 304

BLAST of CmaCh20G008440 vs. NCBI nr
Match: XP_022986575.1 (isoflavone reductase-like protein [Cucurbita maxima] >XP_023512224.1 isoflavone reductase homolog PCBER-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 619.8 bits (1597), Expect = 1.3e-173
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL
Sbjct: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
           DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG
Sbjct: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQFV 309
           EEYLDQFV
Sbjct: 301 EEYLDQFV 308

BLAST of CmaCh20G008440 vs. NCBI nr
Match: KAG6571107.1 (Phenylcoumaran benzylic ether reductase Pyrc5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 617.8 bits (1592), Expect = 4.9e-173
Identity = 307/308 (99.68%), Postives = 307/308 (99.68%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL
Sbjct: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
           DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG
Sbjct: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRP ANIYSTNDLVSLWERKIGKTLRR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPPANIYSTNDLVSLWERKIGKTLRR 240

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQFV 309
           EEYLDQFV
Sbjct: 301 EEYLDQFV 308

BLAST of CmaCh20G008440 vs. NCBI nr
Match: KAG7010928.1 (hypothetical protein SDJN02_27726 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 617.8 bits (1592), Expect = 4.9e-173
Identity = 307/308 (99.68%), Postives = 307/308 (99.68%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL
Sbjct: 304 MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 363

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI
Sbjct: 364 FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 423

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
           DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG
Sbjct: 424 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 483

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRP ANIYSTNDLVSLWERKIGKTLRR
Sbjct: 484 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPPANIYSTNDLVSLWERKIGKTLRR 543

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 544 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 603

Query: 301 EEYLDQFV 309
           EEYLDQFV
Sbjct: 604 EEYLDQFV 611

BLAST of CmaCh20G008440 vs. NCBI nr
Match: XP_022943555.1 (isoflavone reductase-like protein [Cucurbita moschata])

HSP 1 Score: 615.5 bits (1586), Expect = 2.4e-172
Identity = 306/308 (99.35%), Postives = 306/308 (99.35%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGV FL
Sbjct: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVKFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
           DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG
Sbjct: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRP ANIYSTNDLVSLWERKIGKTLRR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPPANIYSTNDLVSLWERKIGKTLRR 240

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQFV 309
           EEYLDQFV
Sbjct: 301 EEYLDQFV 308

BLAST of CmaCh20G008440 vs. NCBI nr
Match: XP_038900572.1 (phenylcoumaran benzylic ether reductase POP1-like [Benincasa hispida])

HSP 1 Score: 590.1 bits (1520), Expect = 1.1e-164
Identity = 287/308 (93.18%), Postives = 301/308 (97.73%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSL S +K +++NHFKSLGVNFL
Sbjct: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLESPSKLRVLNHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFP+EFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
           DAVEPAKSMY AKAEFRR VEAEGIPHTFVVCNFFDGYFLSNLSQPDA+VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DGTPKVIYNKEEDVGTYTIRAI+DPRTLNKIMY+RP ANIYSTNDL+SLWERKIGK+L+R
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAINDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           +YVPEEEVLKNI+E SYPLNIELALCHTAQV+GSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 IYVPEEEVLKNIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQFV 309
           EEYLDQFV
Sbjct: 301 EEYLDQFV 308

BLAST of CmaCh20G008440 vs. TAIR 10
Match: AT4G39230.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 433.3 bits (1113), Expect = 1.6e-121
Identity = 203/308 (65.91%), Postives = 250/308 (81.17%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60
           M    K+L IGGTGYIGK+IV+ASA++GHPT  L+R S+L S ++S  I +FK+LGV FL
Sbjct: 1   MTSKSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120
            GDL D+ SLV +IKQ DVVIST+G  ++ HQ+KI+SAIK+AGNVKRFFP+EFGND D +
Sbjct: 61  LGDLDDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRV 120

Query: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
             VEPAKS Y  KA+ RR +EAEGIP+T+V CNFF GYFL  L+QP A+  PRDKV++LG
Sbjct: 121 FTVEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240
           DG PK ++NKEED+GTYTI A+DDPRTLNKI+YIRP  N YS NDLVSLWE KIGKTL R
Sbjct: 181 DGNPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLER 240

Query: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           +YVPEE++LK I E+S PLN+ L+LCH   V+G  T+F+IEPSFGVEAS LYPDV+YTTV
Sbjct: 241 IYVPEEQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTV 300

Query: 301 EEYLDQFV 309
           +E L+Q+V
Sbjct: 301 DEILNQYV 308

BLAST of CmaCh20G008440 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 375.9 bits (964), Expect = 3.0e-104
Identity = 181/304 (59.54%), Postives = 231/304 (75.99%), Query Frame = 0

Query: 6   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFLFGDLF 65
           K+L+IGGTGYIGKF+V+ASAKAGH T+AL+R+++L    K + +  FK LGV  L GDL 
Sbjct: 7   KILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLN 66

Query: 66  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHIDAVEP 125
           D+ESLVKAIKQVDVVIST+G   +  Q KI+SAIK+AGNVKRF P+EFG D D   AVEP
Sbjct: 67  DHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRTSAVEP 126

Query: 126 AKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQ--PDASVPPRDKVVILGDGT 185
           AKS +  K + RR +EAEGIP+T+ V   F GY+L  L Q  P  + PPRDKV ILGDG 
Sbjct: 127 AKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILGDGN 186

Query: 186 PKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRRVYV 245
            K + NKEED+  YTI+A+DDPRTLNKI+YI+P+ N  S N++V+LWE+KIGK+L + ++
Sbjct: 187 AKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLEKTHL 246

Query: 246 PEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTVEEY 305
           PEE++LK+IQE+  P+N+ L++ H   V G  TN  IEPSFGVEAS LYPDV+YT+V+EY
Sbjct: 247 PEEQLLKSIQESPIPINVVLSINHAVFVNG-DTNISIEPSFGVEASELYPDVKYTSVDEY 306

Query: 306 LDQF 308
           L  F
Sbjct: 307 LSYF 309

BLAST of CmaCh20G008440 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 365.9 bits (938), Expect = 3.1e-101
Identity = 173/308 (56.17%), Postives = 228/308 (74.03%), Query Frame = 0

Query: 2   AQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFLF 61
           ++  K+L+IGGTG+IGK I++AS KAGH T AL+R++SL    K + + +FK  GV  L 
Sbjct: 3   SEKSKILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLH 62

Query: 62  GDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHID 121
           GDL D+ESLVKAIKQ DVVIST+G   +  Q KI+SAIK+AGNVKRF P+EFG D D   
Sbjct: 63  GDLNDHESLVKAIKQADVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDKSS 122

Query: 122 AVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQ--PDASVPPRDKVVIL 181
           AVEPAKS +  K + RR +EAEGIP+T++V N+F GY+L  L Q  P  + PPRDKV I 
Sbjct: 123 AVEPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVKIF 182

Query: 182 GDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLR 241
           GDG  K + NKEED+  YTI+A+DDPRTLNK +YI P  N  S N++V+LWE+KIGK++ 
Sbjct: 183 GDGNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKSVE 242

Query: 242 RVYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTT 301
           ++Y+ EE++ K+IQE+  P N+ L++ H   V+G QTNF IEPSFG EAS LYPD++YT+
Sbjct: 243 KIYMSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKYTS 302

Query: 302 VEEYLDQF 308
           ++EYL  F
Sbjct: 303 IDEYLSYF 310

BLAST of CmaCh20G008440 vs. TAIR 10
Match: AT1G75300.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 355.1 bits (910), Expect = 5.5e-98
Identity = 177/318 (55.66%), Postives = 229/318 (72.01%), Query Frame = 0

Query: 6   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFLFGDLF 65
           K+L+IGGTGY+G+FIV+ SAKAG+PT+AL+R++SL    KS+ I  FK LGV  L GDL 
Sbjct: 7   KILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHGDLN 66

Query: 66  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHIDAVEP 125
           D+ESLVKAIKQVDVVIST+G   +  Q KI+SAIK+AGNVKRF P EFG D +   AVEP
Sbjct: 67  DHESLVKAIKQVDVVISTIGHKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVERTSAVEP 126

Query: 126 AKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQ----------------PDAS 185
           AKS++  K + RR +EAEGIP+T+VV N   G++L  L Q                 D +
Sbjct: 127 AKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAIIFGDKN 186

Query: 186 VPPRDKVVILGDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSL 245
           VPPRDKV ILGDG  KV+ NKEEDV  Y I+A+DD RTLNK +YI P  NI S N++V+L
Sbjct: 187 VPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSMNEMVTL 246

Query: 246 WERKIGKTLRRVYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEAS 305
           WE+KIGK+L + ++ EE++LK+IQ    P+++  ++ H   V+G QT+F IEP FG EAS
Sbjct: 247 WEKKIGKSLEKTHISEEQILKSIQ---VPIDVFKSINHAVFVKGDQTSFTIEPWFGEEAS 306

Query: 306 ALYPDVRYTTVEEYLDQF 308
            LYPDV+YT+++EYL QF
Sbjct: 307 VLYPDVKYTSIDEYLSQF 321

BLAST of CmaCh20G008440 vs. TAIR 10
Match: AT1G19540.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 330.9 bits (847), Expect = 1.1e-90
Identity = 165/307 (53.75%), Postives = 216/307 (70.36%), Query Frame = 0

Query: 6   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFLFGDLF 65
           K+L+IG TG IGK +V+ SAK+GH T+AL+R++SL    K+Q++  FK LGV  L+G L 
Sbjct: 4   KILVIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYGSLS 63

Query: 66  DNESLVKAIKQVDVVISTLGGHM--VPHQHKILSAIKQAGNVKRFFPTEFGNDADHIDAV 125
           D ESLVKAIKQVDVVIS +G     + +Q  I+ AIK++GNVKRF P+EFGND D   A+
Sbjct: 64  DKESLVKAIKQVDVVISAVGRFQTEILNQTNIIDAIKESGNVKRFLPSEFGNDVDRTVAI 123

Query: 126 EPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASV--PPRDKVVILGD 185
           EP  S +  KA+ RR +EA  IP+T+VV   F G F+  L Q    +  PPRDKV I   
Sbjct: 124 EPTLSEFITKAQIRRAIEAAKIPYTYVVSGCFAGLFVPCLGQCHLRLRSPPRDKVSIYDT 183

Query: 186 GTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRRV 245
           G  K I N EED+  YT++A+DDPRTLNKI+YI P   I S ND+V LWE KIGKTL + 
Sbjct: 184 GNGKAIVNTEEDIVAYTLKAVDDPRTLNKILYIHPPNYIVSQNDMVGLWEEKIGKTLEKT 243

Query: 246 YVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTVE 305
           YV EEE+LK IQE+  P++  + L HT  V+   T+F I+PSFGVEAS LYP+V+YT+V+
Sbjct: 244 YVSEEELLKTIQESKPPMDFLVGLIHTILVKSDFTSFTIDPSFGVEASELYPEVKYTSVD 303

Query: 306 EYLDQFV 309
           E+L++F+
Sbjct: 304 EFLNRFI 310

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O813554.0e-12569.48Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC... [more]
B9HRL71.1e-12469.31Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=P... [more]
B2WSN03.5e-12165.70Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1[more]
Q9T0302.2e-12065.91Phenylcoumaran benzylic ether reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PCB... [more]
Q9FUW62.1e-11864.61Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6... [more]
Match NameE-valueIdentityDescription
A0A6J1JEF26.2e-174100.00isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111484277 PE... [more]
A0A6J1FXY61.2e-17299.35isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111448291 ... [more]
A0A1S3CE333.8e-16393.51isoflavone reductase-like protein OS=Cucumis melo OX=3656 GN=LOC103499680 PE=4 S... [more]
A0A5A7TR104.2e-16292.86Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A5D3DYL64.2e-16293.46Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
XP_022986575.11.3e-173100.00isoflavone reductase-like protein [Cucurbita maxima] >XP_023512224.1 isoflavone ... [more]
KAG6571107.14.9e-17399.68Phenylcoumaran benzylic ether reductase Pyrc5, partial [Cucurbita argyrosperma s... [more]
KAG7010928.14.9e-17399.68hypothetical protein SDJN02_27726 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022943555.12.4e-17299.35isoflavone reductase-like protein [Cucurbita moschata][more]
XP_038900572.11.1e-16493.18phenylcoumaran benzylic ether reductase POP1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G39230.11.6e-12165.91NmrA-like negative transcriptional regulator family protein [more]
AT1G75280.13.0e-10459.54NmrA-like negative transcriptional regulator family protein [more]
AT1G75290.13.1e-10156.17NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G75300.15.5e-9855.66NmrA-like negative transcriptional regulator family protein [more]
AT1G19540.11.1e-9053.75NmrA-like negative transcriptional regulator family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 6..294
e-value: 2.4E-132
score: 442.5
NoneNo IPR availableGENE3D3.90.25.10coord: 154..304
e-value: 2.4E-132
score: 442.5
NoneNo IPR availablePANTHERPTHR43349PINORESINOL REDUCTASE-RELATEDcoord: 1..308
NoneNo IPR availablePANTHERPTHR43349:SF35PHENYLCOUMARAN BENZYLIC ETHER REDUCTASE 1coord: 1..308
NoneNo IPR availableCDDcd05259PCBER_SDR_acoord: 6..303
e-value: 5.5292E-83
score: 249.913
IPR008030NmrA-like domainPFAMPF05368NmrAcoord: 7..295
e-value: 2.9E-75
score: 252.8
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 6..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G008440.1CmaCh20G008440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009807 lignan biosynthetic process
molecular_function GO:0016491 oxidoreductase activity