CmaCh20G006250 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G006250
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionseptum-promoting GTP-binding protein 1-like
LocationCma_Chr20: 2921523 .. 2923676 (+)
RNA-Seq ExpressionCmaCh20G006250
SyntenyCmaCh20G006250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATCTCTCACTCCAGGCCATCCATAACGTTTTCCCAACAATGCCTCAACCTTGCCGGAAACAGCCTCTCCGTCTAAGTGTTAAGCGGCAGACCGCTCTCAAGCGCCACGCCGGCCAATTTTTGGACTCGATTCTTGCGTGTTACAGCTCCACCAAGCCTAAGGCGTATCCCGCGGTGCCCACTGAAGCCACTGCGGCTTCTACGGTTGTCGACGATGCTGGGTTTGCGGCGGAGACTCTAACACCATCCGTGTGCCATGATCATGGCCTGGACAAAGATTTGGTGTCTTTGAAAATTAGCCTGTTGGGTGATAGCCAAATTGGAAAGACAAGTTTTCTGGTAATTTGATTTTGTTTTGTTGGCGTTTTGGTTGGTATCTGAGTTTTGTTTTTCATTGTCTGTTTTGAAGGTTAAATATGTTGGGAATGAATTGGATGAAGGAAGGAGTGAGCAGACAGGATTGAATTTGATGGACAAAACACTTATGGTTAGAGGAGCAAGAATTTCTTATAGCATTTGGGAAGTGGAAGGTAATTACACCTTTTACTCTAGTAAAGACACTATAATTAAATTAAAGGCACGACTCTAATAATATTATTGGGAATCATGAATCTCGATATTGTTCACTTTGAGCATAAGCTCTCATGGCTTTGCTTTGAGTTTTCCCGAAAGACCTCATACGAATAAAGATGTATTCCTTACTAACTCATGATCATTTCATCTTGAACAATTACAATTTCAAGACTAAATTTATCGAAGCCCATCAGCTTAAATGATATTAGAACGTAGTTTTGTGTTTGCATTTGTGTATGAACGTTATGAGGATGTGGATGCAGGTGATAAGAAATCTCAAGATTACATTCCAACGGCGTGTAAGGACTCTGTGGCAATTCTTTTTATGTTTGATCTAACAAGTAGGCGTACTCTAACCGGGTGAGGCTTATTTCTGTTTGATCTAAATGACTTTGGTCTTCATGAATGGGAAGAATTAAACATGAGGGTTTTCCCTTTGGTTTGCAGCATTGTAAAGTGGTATAGACAAGCTCGAAAGTATAATCAGGTAGAGTTTAGGACCCGTTTGATAATGTTTTTAGAATGAAGTTTTGAATTTCTTTTTGTCCGTTCAAAGCTCTTTTCTTTGCAGAGAGCGATTCCAGTGTTGGTAGGAACAAAGTTCGATGATTTCATCCAACTCCCCATCGATTTGCAATGGGCAATTGCAAACGAGGTAAGAATTGAAATCGTGAGTGCTTAATAGTAGATATTGTCTTATTTGGGCTTTTCCTTTCACACTTTTCCTCAAAGTTTTTAAGACGGGTCTGTTACTTCTCCTCCACCGATGTAGGATCTCATAGAATGTCTCATGATGTCCTTGTTTTGACAGGCAAGAACTTATGCCAAGGCTTTGAATGCTACACTGTTCTTCTCCAGTTCAACTTACAACATCAATGTCAATAAGATCTTTAAGTTCATCACAGCAAAGCTCTTTGACCTGCCGTGGACGGTCGAGCGCAACTTAACGGTCGGAGAGCCTATAATTGATTTCTAGAGTCCGTCCACTCTACATAGCCAATCTGGTTCTCAAGAAACTCACCAAACCATCCTGCACAAGGATAAACTGTCAACATTGCTTGAACAACCATGTCCGGTGTAGCGGAGAAAGGCAGCGGCGTCGAGCCCCCACCTCCTCAAGTCGAAATGCATCTGCATTTTTGACTGCACCAAGGTTGTAGAAAAGCAATAAGACAATGAAGAAAAGAACAAGCACACTTGGGTTACAGTTCATGGGAACCAGGTCAGGTATATAATTGCAAAGGCAGCAGCTTTTGAATCCCTTCAAGAAGTTTAGATCTTCTAACTCCATGAATATCTGTTTATCACGCTCTCAACATCTTCTCTTAGGCGAGAAAATTGAAGTGGTCTGTCAAATTCAACAGCAGTTAGATGTCATTGCTTGATGTGATCAAATAATGTTTTGTCCTTAAGCTTTAGGAAGTCAGTCATGGTTAAAACACAAGAAGTCTTCTTCAATCATAGCCCACCCTGTGGCTAGGCGAGTCGAGTCGTATAAATCAAGAGAAAGCAATGAGGCTGCTATGGAAAATTATCTTTATTGGATGTATGAGAATAATCTACAAGTTACTAAGAGTATG

mRNA sequence

CAATCTCTCACTCCAGGCCATCCATAACGTTTTCCCAACAATGCCTCAACCTTGCCGGAAACAGCCTCTCCGTCTAAGTGTTAAGCGGCAGACCGCTCTCAAGCGCCACGCCGGCCAATTTTTGGACTCGATTCTTGCGTGTTACAGCTCCACCAAGCCTAAGGCGTATCCCGCGGTGCCCACTGAAGCCACTGCGGCTTCTACGGTTGTCGACGATGCTGGGTTTGCGGCGGAGACTCTAACACCATCCGTGTGCCATGATCATGGCCTGGACAAAGATTTGGTGTCTTTGAAAATTAGCCTGTTGGGTGATAGCCAAATTGGAAAGACAAGTTTTCTGGTTAAATATGTTGGGAATGAATTGGATGAAGGAAGGAGTGAGCAGACAGGATTGAATTTGATGGACAAAACACTTATGGTTAGAGGAGCAAGAATTTCTTATAGCATTTGGGAAGTGGAAGGTGATAAGAAATCTCAAGATTACATTCCAACGGCGTGTAAGGACTCTGTGGCAATTCTTTTTATGTTTGATCTAACAAGTAGGCGTACTCTAACCGGCATTGTAAAGTGGTATAGACAAGCTCGAAAGTATAATCAGAGAGCGATTCCAGTGTTGGTAGGAACAAAGTTCGATGATTTCATCCAACTCCCCATCGATTTGCAATGGGCAATTGCAAACGAGGCAAGAACTTATGCCAAGGCTTTGAATGCTACACTGTTCTTCTCCAGTTCAACTTACAACATCAATGTCAATAAGATCTTTAAGTTCATCACAGCAAAGCTCTTTGACCTGCCGTGGACGGTCGAGCGCAACTTAACGGTCGGAGAGCCTATAATTGATTTCTAGAGTCCGTCCACTCTACATAGCCAATCTGGTTCTCAAGAAACTCACCAAACCATCCTGCACAAGGATAAACTGTCAACATTGCTTGAACAACCATGTCCGGTGTAGCGGAGAAAGGCAGCGGCGTCGAGCCCCCACCTCCTCAAGTCGAAATGCATCTGCATTTTTGACTGCACCAAGGTTGTAGAAAAGCAATAAGACAATGAAGAAAAGAACAAGCACACTTGGGTTACAGTTCATGGGAACCAGGTCAGGTATATAATTGCAAAGGCAGCAGCTTTTGAATCCCTTCAAGAAGTTTAGATCTTCTAACTCCATGAATATCTGTTTATCACGCTCTCAACATCTTCTCTTAGGCGAGAAAATTGAAGTGGTCTGTCAAATTCAACAGCAGTTAGATGTCATTGCTTGATGTGATCAAATAATGTTTTGTCCTTAAGCTTTAGGAAGTCAGTCATGGTTAAAACACAAGAAGTCTTCTTCAATCATAGCCCACCCTGTGGCTAGGCGAGTCGAGTCGTATAAATCAAGAGAAAGCAATGAGGCTGCTATGGAAAATTATCTTTATTGGATGTATGAGAATAATCTACAAGTTACTAAGAGTATG

Coding sequence (CDS)

ATGCCTCAACCTTGCCGGAAACAGCCTCTCCGTCTAAGTGTTAAGCGGCAGACCGCTCTCAAGCGCCACGCCGGCCAATTTTTGGACTCGATTCTTGCGTGTTACAGCTCCACCAAGCCTAAGGCGTATCCCGCGGTGCCCACTGAAGCCACTGCGGCTTCTACGGTTGTCGACGATGCTGGGTTTGCGGCGGAGACTCTAACACCATCCGTGTGCCATGATCATGGCCTGGACAAAGATTTGGTGTCTTTGAAAATTAGCCTGTTGGGTGATAGCCAAATTGGAAAGACAAGTTTTCTGGTTAAATATGTTGGGAATGAATTGGATGAAGGAAGGAGTGAGCAGACAGGATTGAATTTGATGGACAAAACACTTATGGTTAGAGGAGCAAGAATTTCTTATAGCATTTGGGAAGTGGAAGGTGATAAGAAATCTCAAGATTACATTCCAACGGCGTGTAAGGACTCTGTGGCAATTCTTTTTATGTTTGATCTAACAAGTAGGCGTACTCTAACCGGCATTGTAAAGTGGTATAGACAAGCTCGAAAGTATAATCAGAGAGCGATTCCAGTGTTGGTAGGAACAAAGTTCGATGATTTCATCCAACTCCCCATCGATTTGCAATGGGCAATTGCAAACGAGGCAAGAACTTATGCCAAGGCTTTGAATGCTACACTGTTCTTCTCCAGTTCAACTTACAACATCAATGTCAATAAGATCTTTAAGTTCATCACAGCAAAGCTCTTTGACCTGCCGTGGACGGTCGAGCGCAACTTAACGGTCGGAGAGCCTATAATTGATTTCTAG

Protein sequence

MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDAGFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNLMDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF
Homology
BLAST of CmaCh20G006250 vs. ExPASy Swiss-Prot
Match: P87027 (Septum-promoting GTP-binding protein 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=spg1 PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 4.0e-49
Identity = 87/190 (45.79%), Postives = 134/190 (70.53%), Query Frame = 0

Query: 79  KDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNLMDKTLMVRGARISYSIWE 138
           K+ V++K+ ++GDS IGKTS +V YV    DE  ++  G+N M+KT+ +R   I++SIW+
Sbjct: 6   KNNVTIKVGMIGDSSIGKTSLMVTYVQGSFDEESTQTLGVNFMEKTISIRNTEITFSIWD 65

Query: 139 VEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYNQRAIPVLVGTKFD 198
           + G ++  + +P  C D+VAILFMFDL+ + TL  I +WYRQAR +N+ A+P+L+GTK+D
Sbjct: 66  LGGQREFVNMLPMVCNDAVAILFMFDLSRKSTLNSIKEWYRQARGFNKTAVPILIGTKYD 125

Query: 199 DFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKIFKFITAKLFDLPWTVERN 258
            F+  P + Q  I  +AR YAKA+ A+L F S++++INV KIFK + AK+FDL  T+   
Sbjct: 126 HFMTFPREDQEEITKQARRYAKAMKASLVFCSTSHSINVQKIFKIVLAKVFDLKCTIPEI 185

Query: 259 LTVGEPIIDF 269
             VG+PI+++
Sbjct: 186 KNVGDPILEY 195

BLAST of CmaCh20G006250 vs. ExPASy Swiss-Prot
Match: P38987 (Protein TEM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TEM1 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.1e-35
Identity = 76/201 (37.81%), Postives = 124/201 (61.69%), Query Frame = 0

Query: 68  TPSVCHDHGLD--KDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNLMDKTL 127
           TPS   ++ +   ++ V +++ L+GD+Q+GKTS +VKYV N  D+  ++  G+N + + +
Sbjct: 3   TPSTGANNSIPAVRNQVEVQVGLVGDAQVGKTSLMVKYVQNIYDKEYTQTLGVNFLKRKV 62

Query: 128 MVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYN 187
            +R   I +SI ++ G ++  + +P A   S  I+F+FDLT   TL+ I +WYRQA   N
Sbjct: 63  SIRSTDIIFSIMDLGGQREFINMLPIATVGSSVIIFLFDLTRPETLSSIKEWYRQAYGLN 122

Query: 188 QRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKIFKFIT 247
             AIP+LVGTK+D  I L  + Q  I+  +  YA+ +NA L F S+  +IN+ KIFK   
Sbjct: 123 DSAIPILVGTKYDLLIDLDPEYQEQISRTSMKYAQVMNAPLIFCSTAKSINIQKIFKIAL 182

Query: 248 AKLFDLPWTVERNLTVGEPII 267
           AK+F+L  T+     +G+P++
Sbjct: 183 AKIFNLTLTIPEINEIGDPLL 203

BLAST of CmaCh20G006250 vs. ExPASy Swiss-Prot
Match: Q9VP48 (Ras-related protein Rab-26 OS=Drosophila melanogaster OX=7227 GN=Rab26 PE=2 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 5.9e-16
Identity = 49/132 (37.12%), Postives = 72/132 (54.55%), Query Frame = 0

Query: 69  PSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGR-SEQTGLNLMDKTLMV 128
           PS  H    D D V+ K  LLGDS +GKTSFLVKY   E   G  S   G+ L +K ++V
Sbjct: 177 PSNAHQQPFD-DTVNHKTILLGDSGVGKTSFLVKYNTGEFRLGSFSATVGIALTNKVVVV 236

Query: 129 RGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYNQR 188
            G R+   IW+  G ++ +       +D+ A+L ++D+T++ T   I  W  + R+Y Q 
Sbjct: 237 DGTRVKLQIWDTAGQERFRSVTHAYYRDAHALLLLYDVTNKTTYDNIRAWLGEIREYAQE 296

Query: 189 -AIPVLVGTKFD 199
             + VL+G K D
Sbjct: 297 DVVIVLIGNKAD 307

BLAST of CmaCh20G006250 vs. ExPASy Swiss-Prot
Match: P07560 (Ras-related protein SEC4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEC4 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 7.6e-16
Identity = 54/196 (27.55%), Postives = 100/196 (51.02%), Query Frame = 0

Query: 68  TPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNLMDKTLMV 127
           T S    +G   D + +KI L+GDS +GK+  LV++V ++ +       G++   KT+ +
Sbjct: 6   TVSASSGNGKSYDSI-MKILLIGDSGVGKSCLLVRFVEDKFNPSFITTIGIDFKIKTVDI 65

Query: 128 RGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKY-NQ 187
            G ++   +W+  G ++ +       + ++ I+ ++D+T  RT T I +W++   ++ N 
Sbjct: 66  NGKKVKLQLWDTAGQERFRTITTAYYRGAMGIILVYDVTDERTFTNIKQWFKTVNEHAND 125

Query: 188 RAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKIFKFITA 247
            A  +LVG K D      ++ +   A++    AK L      SS+  + NVN+IF F  A
Sbjct: 126 EAQLLLVGNKSD------METRVVTADQGEALAKELGIPFIESSAKNDDNVNEIF-FTLA 185

Query: 248 KLFDLPWTVERNLTVG 263
           KL  +   ++ N  VG
Sbjct: 186 KL--IQEKIDSNKLVG 191

BLAST of CmaCh20G006250 vs. ExPASy Swiss-Prot
Match: O80501 (Ras-related protein RABH1b OS=Arabidopsis thaliana OX=3702 GN=RABH1B PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.9e-15
Identity = 44/169 (26.04%), Postives = 81/169 (47.93%), Query Frame = 0

Query: 81  LVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNLMDKTLMVRGARISYSIWEVE 140
           L   K+  LGD  +GKTS + +++ ++ D       G++ + KT+ +    +   +W+  
Sbjct: 7   LAKYKLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTA 66

Query: 141 GDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQAR-KYNQRAIPVLVGTKFDD 200
           G ++ +  IP+  +DS   + ++D+ SR++     KW  + R +     I VLVG K D 
Sbjct: 67  GQERFRSLIPSYIRDSSVAVIVYDVASRQSFLNTTKWIDEVRTERGSDVIVVLVGNKTD- 126

Query: 201 FIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKIFKFITAKL 249
                +D +     EA   A+ LN     +S+    N+  +F+ I A L
Sbjct: 127 ----LVDKRQVSIEEAEAKARELNVMFIETSAKAGFNIKALFRKIAAAL 170

BLAST of CmaCh20G006250 vs. ExPASy TrEMBL
Match: A0A6J1JFU6 (septum-promoting GTP-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111484119 PE=4 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.0e-148
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDA 60
           MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDA
Sbjct: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDA 60

Query: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120
           GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL
Sbjct: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120

Query: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180
           MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ
Sbjct: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180

Query: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKI 240
           ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKI
Sbjct: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKI 240

Query: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 269
           FKFITAKLFDLPWTVERNLTVGEPIIDF
Sbjct: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 268

BLAST of CmaCh20G006250 vs. ExPASy TrEMBL
Match: A0A6J1FYS5 (septum-promoting GTP-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449038 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 3.7e-146
Identity = 263/268 (98.13%), Postives = 266/268 (99.25%), Query Frame = 0

Query: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDA 60
           MPQPCRKQPLRLSVKRQTALKRHAGQFLDSIL+CYSSTKPKAYPAVP EATAASTVV+DA
Sbjct: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILSCYSSTKPKAYPAVPDEATAASTVVEDA 60

Query: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120
           GFAAE+LTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL
Sbjct: 61  GFAAESLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120

Query: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180
           MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ
Sbjct: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180

Query: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKI 240
           ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEAR YAKALNATLFFSSSTYNINVNKI
Sbjct: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARAYAKALNATLFFSSSTYNINVNKI 240

Query: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 269
           FKFITAKLFDLPWTVERNLTVGEPIIDF
Sbjct: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 268

BLAST of CmaCh20G006250 vs. ExPASy TrEMBL
Match: A0A5A7TPV3 (Septum-promoting GTP-binding protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold27G00460 PE=4 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 4.5e-112
Identity = 211/269 (78.44%), Postives = 234/269 (86.99%), Query Frame = 0

Query: 1   MPQPCRKQPL-RLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDD 60
           MP+P RK PL R +++R+TALKR A  F DSIL CYSS KP+AY  V   +T  S VV D
Sbjct: 1   MPRPSRKHPLRRFTLRRRTALKRRAWHFFDSILTCYSSKKPQAYDDVALAST--SVVVVD 60

Query: 61  AGFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLN 120
             F AETL PS+CH    ++DLVSLKISLLGDSQIGKTSFLVKYVGNE+DEG+SEQTGL 
Sbjct: 61  EEFVAETLAPSLCH----NQDLVSLKISLLGDSQIGKTSFLVKYVGNEMDEGKSEQTGLK 120

Query: 121 LMDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYR 180
           LMDKTLMVRGARISYSIWEVEGD KSQDYI TACK SVAIL+MFDLTSRRTL   V+WYR
Sbjct: 121 LMDKTLMVRGARISYSIWEVEGDSKSQDYISTACKGSVAILYMFDLTSRRTLNNTVRWYR 180

Query: 181 QARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNK 240
           QAR++N+ AIPVL+GTKFDDFIQLPIDLQWAIA+EAR+YAKALNATLFFSS+TYNINVNK
Sbjct: 181 QARRWNKTAIPVLIGTKFDDFIQLPIDLQWAIASEARSYAKALNATLFFSSATYNINVNK 240

Query: 241 IFKFITAKLFDLPWTVERNLTVGEPIIDF 269
           IFKFITAKLFDLPWT+ERNLT+GEPIIDF
Sbjct: 241 IFKFITAKLFDLPWTIERNLTIGEPIIDF 263

BLAST of CmaCh20G006250 vs. ExPASy TrEMBL
Match: A0A1S3C1V5 (septum-promoting GTP-binding protein 1 OS=Cucumis melo OX=3656 GN=LOC103495951 PE=4 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 4.5e-112
Identity = 211/269 (78.44%), Postives = 234/269 (86.99%), Query Frame = 0

Query: 1   MPQPCRKQPL-RLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDD 60
           MP+P RK PL R +++R+TALKR A  F DSIL CYSS KP+AY  V   +T  S VV D
Sbjct: 1   MPRPSRKHPLRRFTLRRRTALKRRAWHFFDSILTCYSSKKPQAYDDVALAST--SVVVVD 60

Query: 61  AGFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLN 120
             F AETL PS+CH    ++DLVSLKISLLGDSQIGKTSFLVKYVGNE+DEG+SEQTGL 
Sbjct: 61  EEFVAETLAPSLCH----NQDLVSLKISLLGDSQIGKTSFLVKYVGNEMDEGKSEQTGLK 120

Query: 121 LMDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYR 180
           LMDKTLMVRGARISYSIWEVEGD KSQDYI TACK SVAIL+MFDLTSRRTL   V+WYR
Sbjct: 121 LMDKTLMVRGARISYSIWEVEGDSKSQDYISTACKGSVAILYMFDLTSRRTLNNTVRWYR 180

Query: 181 QARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNK 240
           QAR++N+ AIPVL+GTKFDDFIQLPIDLQWAIA+EAR+YAKALNATLFFSS+TYNINVNK
Sbjct: 181 QARRWNKTAIPVLIGTKFDDFIQLPIDLQWAIASEARSYAKALNATLFFSSATYNINVNK 240

Query: 241 IFKFITAKLFDLPWTVERNLTVGEPIIDF 269
           IFKFITAKLFDLPWT+ERNLT+GEPIIDF
Sbjct: 241 IFKFITAKLFDLPWTIERNLTIGEPIIDF 263

BLAST of CmaCh20G006250 vs. ExPASy TrEMBL
Match: A0A6J1I4P4 (septum-promoting GTP-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111469846 PE=4 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.3e-111
Identity = 214/276 (77.54%), Postives = 232/276 (84.06%), Query Frame = 0

Query: 1   MPQPCRKQPLRLSVKRQ-----TALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAAS- 60
           MPQPCRKQPL   +KRQ     TALKR   QFL+SIL CYS  KP+    V  +A  AS 
Sbjct: 19  MPQPCRKQPLNFGLKRQMHHPATALKRRTRQFLESILTCYSK-KPQTCRTVRDDAATASI 78

Query: 61  --TVVDDAGFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGR 120
               V D GF AET TP +CH+H  DKDLV LKISLLGDSQIGKT FL KYVGNE++EG+
Sbjct: 79  SPRAVADGGFEAETRTP-LCHNHSQDKDLVPLKISLLGDSQIGKTCFLEKYVGNEMEEGK 138

Query: 121 SEQTGLNLMDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLT 180
           SEQ GLNLMDKTLMVRGARISYSIWEV GDKKSQD+IPTACKDSVAILFMFDLTSRRTL 
Sbjct: 139 SEQRGLNLMDKTLMVRGARISYSIWEVGGDKKSQDHIPTACKDSVAILFMFDLTSRRTLK 198

Query: 181 GIVKWYRQARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSST 240
            +++WYRQA K+NQ AIPVL+GTKFDDFIQLPIDLQWAIA EARTYAKALNATLFFSS+T
Sbjct: 199 SVLRWYRQAWKWNQAAIPVLIGTKFDDFIQLPIDLQWAIACEARTYAKALNATLFFSSAT 258

Query: 241 YNINVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 269
           YNINVNKIFKFITAKLFDLPWT+ERNLTVGEP+IDF
Sbjct: 259 YNINVNKIFKFITAKLFDLPWTLERNLTVGEPMIDF 292

BLAST of CmaCh20G006250 vs. NCBI nr
Match: XP_022986354.1 (septum-promoting GTP-binding protein 1-like [Cucurbita maxima])

HSP 1 Score: 535.8 bits (1379), Expect = 2.1e-148
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDA 60
           MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDA
Sbjct: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDA 60

Query: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120
           GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL
Sbjct: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120

Query: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180
           MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ
Sbjct: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180

Query: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKI 240
           ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKI
Sbjct: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKI 240

Query: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 269
           FKFITAKLFDLPWTVERNLTVGEPIIDF
Sbjct: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 268

BLAST of CmaCh20G006250 vs. NCBI nr
Match: XP_022944635.1 (septum-promoting GTP-binding protein 1-like [Cucurbita moschata])

HSP 1 Score: 527.3 bits (1357), Expect = 7.6e-146
Identity = 263/268 (98.13%), Postives = 266/268 (99.25%), Query Frame = 0

Query: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDA 60
           MPQPCRKQPLRLSVKRQTALKRHAGQFLDSIL+CYSSTKPKAYPAVP EATAASTVV+DA
Sbjct: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILSCYSSTKPKAYPAVPDEATAASTVVEDA 60

Query: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120
           GFAAE+LTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL
Sbjct: 61  GFAAESLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120

Query: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180
           MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ
Sbjct: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180

Query: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKI 240
           ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEAR YAKALNATLFFSSSTYNINVNKI
Sbjct: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARAYAKALNATLFFSSSTYNINVNKI 240

Query: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 269
           FKFITAKLFDLPWTVERNLTVGEPIIDF
Sbjct: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 268

BLAST of CmaCh20G006250 vs. NCBI nr
Match: XP_023513332.1 (septum-promoting GTP-binding protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.0 bits (1351), Expect = 3.8e-145
Identity = 262/268 (97.76%), Postives = 265/268 (98.88%), Query Frame = 0

Query: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDA 60
           MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVP +ATAASTVV+DA
Sbjct: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPDDATAASTVVEDA 60

Query: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120
           GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL
Sbjct: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120

Query: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180
           MDKTL+VRGARISYSIWEV GDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ
Sbjct: 121 MDKTLLVRGARISYSIWEVGGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180

Query: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKI 240
           ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEAR YAKALNATLFFSSSTYNINVNKI
Sbjct: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARAYAKALNATLFFSSSTYNINVNKI 240

Query: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 269
           FKFITAKLFDLPWTVERNLTVGEPIIDF
Sbjct: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 268

BLAST of CmaCh20G006250 vs. NCBI nr
Match: KAG6570880.1 (Glutamate receptor 3.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 523.9 bits (1348), Expect = 8.4e-145
Identity = 262/268 (97.76%), Postives = 265/268 (98.88%), Query Frame = 0

Query: 1    MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDA 60
            MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAY AVP +ATAASTVV+DA
Sbjct: 898  MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYAAVPDDATAASTVVEDA 957

Query: 61   GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120
            GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL
Sbjct: 958  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 1017

Query: 121  MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180
            MDKTLMVRGARISYSIWEVEG+KKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ
Sbjct: 1018 MDKTLMVRGARISYSIWEVEGNKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 1077

Query: 181  ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKI 240
            ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEAR YAKALNATLFFSSSTYNINVNKI
Sbjct: 1078 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARAYAKALNATLFFSSSTYNINVNKI 1137

Query: 241  FKFITAKLFDLPWTVERNLTVGEPIIDF 269
            FKFITAKLFDLPWTVERNLTVGEPIIDF
Sbjct: 1138 FKFITAKLFDLPWTVERNLTVGEPIIDF 1165

BLAST of CmaCh20G006250 vs. NCBI nr
Match: XP_038901498.1 (septum-promoting GTP-binding protein 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 418.7 bits (1075), Expect = 3.8e-113
Identity = 214/269 (79.55%), Postives = 235/269 (87.36%), Query Frame = 0

Query: 1   MPQPCRKQPL-RLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDD 60
           MP P RK PL R S++R+TALKR A  F DSIL CY+S KPK Y   P +  A S V   
Sbjct: 1   MPHPSRKHPLRRFSLRRRTALKRRAWHFFDSILTCYNSKKPKTYR--PDDDGATSVV--G 60

Query: 61  AGFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLN 120
           A FAAETLTPS+CH    ++DLVSLKISLLGDSQIGKTSFLVKYVGNE+DEG+SEQTGL 
Sbjct: 61  ADFAAETLTPSLCH----NQDLVSLKISLLGDSQIGKTSFLVKYVGNEMDEGKSEQTGLT 120

Query: 121 LMDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYR 180
           LMDKTLMVRGARISYSIWEVEGD KSQD+IPTACKDSVAILFMFDLTSRRTL  I++WYR
Sbjct: 121 LMDKTLMVRGARISYSIWEVEGDDKSQDFIPTACKDSVAILFMFDLTSRRTLNNIIRWYR 180

Query: 181 QARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNK 240
           QAR++NQ AIPVL+GTKFDDFIQLPID+QWAIA+EAR YAKALNATLFFSS+TYNINVNK
Sbjct: 181 QARRWNQTAIPVLIGTKFDDFIQLPIDMQWAIASEARAYAKALNATLFFSSATYNINVNK 240

Query: 241 IFKFITAKLFDLPWTVERNLTVGEPIIDF 269
           IFKFITAKLFDLPWT+ERNLT+GEPIIDF
Sbjct: 241 IFKFITAKLFDLPWTIERNLTIGEPIIDF 261

BLAST of CmaCh20G006250 vs. TAIR 10
Match: AT3G21700.3 (Ras-related small GTP-binding family protein )

HSP 1 Score: 303.1 bits (775), Expect = 2.2e-82
Identity = 172/292 (58.90%), Postives = 202/292 (69.18%), Query Frame = 0

Query: 1   MPQPCRK----QPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAY---------PAV- 60
           M Q C K      LR  V R+  + R   + L S +   +  K + Y         P V 
Sbjct: 1   MAQSCLKIVRINNLRNRVNRRILILRRFTRLLWSRIVACTPGKSRRYLLLSRALPSPTVS 60

Query: 61  ---PTEATAASTVVDDAGFAAETL-TPSVCHDHG-----LDKDLVSLKISLLGDSQIGKT 120
              P+   A   VV   G   E +   SV +D+       D DLVSLKISLLGD +IGKT
Sbjct: 61  RPSPSPIPAVDVVVGGGGGGGEFVRRSSVVYDNDNSHRRSDSDLVSLKISLLGDPEIGKT 120

Query: 121 SFLVKYVGNELD-EGRSEQTGLNLMDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDS 180
           SFL KYVG E + E R  + G+N  DKTL + GARISYSIWE+EG ++S+D IP ACKDS
Sbjct: 121 SFLAKYVGEEKEVEMRELEKGINCTDKTLYMGGARISYSIWELEGAERSRDQIPVACKDS 180

Query: 181 VAILFMFDLTSRRTLTGIVKWYRQARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEAR 240
           VAILFMFDLTSR TL  ++ WY+QARK NQ AIPV+VGTKFD+FIQLPIDLQW IA++AR
Sbjct: 181 VAILFMFDLTSRCTLNSVISWYQQARKSNQTAIPVMVGTKFDEFIQLPIDLQWTIASQAR 240

Query: 241 TYAKALNATLFFSSSTYNINVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 269
           TYAKALNATLFFSS++YNINVNKIFKF+TAKLFDLPWTVERNLT+GEPIIDF
Sbjct: 241 TYAKALNATLFFSSASYNINVNKIFKFVTAKLFDLPWTVERNLTIGEPIIDF 292

BLAST of CmaCh20G006250 vs. TAIR 10
Match: AT3G21700.1 (Ras-related small GTP-binding family protein )

HSP 1 Score: 297.4 bits (760), Expect = 1.2e-80
Identity = 171/292 (58.56%), Postives = 201/292 (68.84%), Query Frame = 0

Query: 1   MPQPCRK----QPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAY---------PAV- 60
           M Q C K      LR  V R+  + R   + L S +   +  K + Y         P V 
Sbjct: 1   MAQSCLKIVRINNLRNRVNRRILILRRFTRLLWSRIVACTPGKSRRYLLLSRALPSPTVS 60

Query: 61  ---PTEATAASTVVDDAGFAAETL-TPSVCHDHG-----LDKDLVSLKISLLGDSQIGKT 120
              P+   A   VV   G   E +   SV +D+       D DLVSLKISLLGD +IGKT
Sbjct: 61  RPSPSPIPAVDVVVGGGGGGGEFVRRSSVVYDNDNSHRRSDSDLVSLKISLLGDPEIGKT 120

Query: 121 SFLVKYVGNELD-EGRSEQTGLNLMDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDS 180
           SFL KYVG E + E R  + G+N  DKTL + GARISYSIWE+E + +S+D IP ACKDS
Sbjct: 121 SFLAKYVGEEKEVEMRELEKGINCTDKTLYMGGARISYSIWELEAE-RSRDQIPVACKDS 180

Query: 181 VAILFMFDLTSRRTLTGIVKWYRQARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEAR 240
           VAILFMFDLTSR TL  ++ WY+QARK NQ AIPV+VGTKFD+FIQLPIDLQW IA++AR
Sbjct: 181 VAILFMFDLTSRCTLNSVISWYQQARKSNQTAIPVMVGTKFDEFIQLPIDLQWTIASQAR 240

Query: 241 TYAKALNATLFFSSSTYNINVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 269
           TYAKALNATLFFSS++YNINVNKIFKF+TAKLFDLPWTVERNLT+GEPIIDF
Sbjct: 241 TYAKALNATLFFSSASYNINVNKIFKFVTAKLFDLPWTVERNLTIGEPIIDF 291

BLAST of CmaCh20G006250 vs. TAIR 10
Match: AT5G54840.1 (Ras-related small GTP-binding family protein )

HSP 1 Score: 275.0 bits (702), Expect = 6.3e-74
Identity = 131/192 (68.23%), Postives = 162/192 (84.38%), Query Frame = 0

Query: 78  DKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNLMDKTLMVRGARISYSIW 137
           D DLVSLKISLLGD Q GKT+F+VKYVG+E ++   E TGLNLMDKT  V+G  IS+SIW
Sbjct: 96  DSDLVSLKISLLGDCQTGKTTFVVKYVGDE-NQSFLEMTGLNLMDKTFYVQGVTISFSIW 155

Query: 138 EVEGD-KKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYNQRAIPVLVGTK 197
           +V GD K+S+D+IP ACKD+VAILFMFDLTSR TL  +  WY QARK+N+ AIP+L+GTK
Sbjct: 156 DVGGDEKRSKDHIPIACKDAVAILFMFDLTSRSTLNSVFGWYSQARKWNKTAIPILIGTK 215

Query: 198 FDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKIFKFITAKLFDLPWTVE 257
           FDDF++LP +LQW I  +AR YAK +NA+LFFSS+T+NINVNKIFKFI A+LF+LPW ++
Sbjct: 216 FDDFVRLPPNLQWTIVTQARAYAKVMNASLFFSSATHNINVNKIFKFILARLFNLPWKID 275

Query: 258 RNLTVGEPIIDF 269
           RNLT+GEPIID+
Sbjct: 276 RNLTLGEPIIDY 286

BLAST of CmaCh20G006250 vs. TAIR 10
Match: AT3G21700.2 (Ras-related small GTP-binding family protein )

HSP 1 Score: 194.1 bits (492), Expect = 1.4e-49
Identity = 121/238 (50.84%), Postives = 147/238 (61.76%), Query Frame = 0

Query: 1   MPQPCRK----QPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAY---------PAV- 60
           M Q C K      LR  V R+  + R   + L S +   +  K + Y         P V 
Sbjct: 1   MAQSCLKIVRINNLRNRVNRRILILRRFTRLLWSRIVACTPGKSRRYLLLSRALPSPTVS 60

Query: 61  ---PTEATAASTVVDDAGFAAETL-TPSVCHDHG-----LDKDLVSLKISLLGDSQIGKT 120
              P+   A   VV   G   E +   SV +D+       D DLVSLKISLLGD +IGKT
Sbjct: 61  RPSPSPIPAVDVVVGGGGGGGEFVRRSSVVYDNDNSHRRSDSDLVSLKISLLGDPEIGKT 120

Query: 121 SFLVKYVGNELD-EGRSEQTGLNLMDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDS 180
           SFL KYVG E + E R  + G+N  DKTL + GARISYSIWE+E + +S+D IP ACKDS
Sbjct: 121 SFLAKYVGEEKEVEMRELEKGINCTDKTLYMGGARISYSIWELEAE-RSRDQIPVACKDS 180

Query: 181 VAILFMFDLTSRRTLTGIVKWYRQARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANE 215
           VAILFMFDLTSR TL  ++ WY+QARK NQ AIPV+VGTKFD+FIQLPIDLQW IA++
Sbjct: 181 VAILFMFDLTSRCTLNSVISWYQQARKSNQTAIPVMVGTKFDEFIQLPIDLQWTIASQ 237

BLAST of CmaCh20G006250 vs. TAIR 10
Match: AT5G54840.2 (Ras-related small GTP-binding family protein )

HSP 1 Score: 147.9 bits (372), Expect = 1.2e-35
Identity = 74/110 (67.27%), Postives = 89/110 (80.91%), Query Frame = 0

Query: 78  DKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNLMDKTLMVRGARISYSIW 137
           D DLVSLKISLLGD Q GKT+F+VKYVG+E ++   E TGLNLMDKT  V+G  IS+SIW
Sbjct: 96  DSDLVSLKISLLGDCQTGKTTFVVKYVGDE-NQSFLEMTGLNLMDKTFYVQGVTISFSIW 155

Query: 138 EVEGD-KKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQARKYNQ 187
           +V GD K+S+D+IP ACKD+VAILFMFDLTSR TL  +  WY QARK+N+
Sbjct: 156 DVGGDEKRSKDHIPIACKDAVAILFMFDLTSRSTLNSVFGWYSQARKWNK 204

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P870274.0e-4945.79Septum-promoting GTP-binding protein 1 OS=Schizosaccharomyces pombe (strain 972 ... [more]
P389871.1e-3537.81Protein TEM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 ... [more]
Q9VP485.9e-1637.12Ras-related protein Rab-26 OS=Drosophila melanogaster OX=7227 GN=Rab26 PE=2 SV=2[more]
P075607.6e-1627.55Ras-related protein SEC4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c... [more]
O805012.9e-1526.04Ras-related protein RABH1b OS=Arabidopsis thaliana OX=3702 GN=RABH1B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JFU61.0e-148100.00septum-promoting GTP-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1FYS53.7e-14698.13septum-promoting GTP-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A5A7TPV34.5e-11278.44Septum-promoting GTP-binding protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3C1V54.5e-11278.44septum-promoting GTP-binding protein 1 OS=Cucumis melo OX=3656 GN=LOC103495951 P... [more]
A0A6J1I4P41.3e-11177.54septum-promoting GTP-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_022986354.12.1e-148100.00septum-promoting GTP-binding protein 1-like [Cucurbita maxima][more]
XP_022944635.17.6e-14698.13septum-promoting GTP-binding protein 1-like [Cucurbita moschata][more]
XP_023513332.13.8e-14597.76septum-promoting GTP-binding protein 1-like [Cucurbita pepo subsp. pepo][more]
KAG6570880.18.4e-14597.76Glutamate receptor 3.4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038901498.13.8e-11379.55septum-promoting GTP-binding protein 1 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G21700.32.2e-8258.90Ras-related small GTP-binding family protein [more]
AT3G21700.11.2e-8058.56Ras-related small GTP-binding family protein [more]
AT5G54840.16.3e-7468.23Ras-related small GTP-binding family protein [more]
AT3G21700.21.4e-4950.84Ras-related small GTP-binding family protein [more]
AT5G54840.21.2e-3567.27Ras-related small GTP-binding family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00449RASTRNSFRMNGcoord: 125..147
score: 17.79
coord: 84..105
score: 34.75
coord: 226..248
score: 21.56
coord: 186..199
score: 35.77
NoneNo IPR availableSMARTSM00175rab_sub_5coord: 84..251
e-value: 2.1E-6
score: -29.7
NoneNo IPR availablePANTHERPTHR47978:SF29MONOMERIC G-PROTEINcoord: 36..267
NoneNo IPR availablePANTHERPTHR47978FAMILY NOT NAMEDcoord: 36..267
NoneNo IPR availablePROSITEPS51419RABcoord: 78..268
score: 12.40694
IPR001806Small GTPasePFAMPF00071Rascoord: 85..248
e-value: 1.9E-21
score: 76.3
IPR017231Small GTPase Tem1/Spg1PIRSFPIRSF037527GTPase_Tem1coord: 3..268
e-value: 3.8E-94
score: 313.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 78..258
e-value: 3.2E-30
score: 106.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 84..259

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G006250.1CmaCh20G006250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0090404 pollen tube tip
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding