CmaCh20G006110 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh20G006110
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Membrane metalloprotease ARASP, chloroplastic
Location	Cma_Chr20: 2855344 .. 2856693 (-)
RNA-Seq Expression	CmaCh20G006110
Synteny	CmaCh20G006110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTATTAAATCTCTCTTCCTCTTCTTATTCAGTCATTAAATTTGCCAATTCGAAGTCACCCTTCTCAGATTTTTCAGTCAAGTACAGAACCCACTTCTCTAAATCCCAATTTTCTTCTTCATTATGTCATTCTTTAACGTCATCGGAGCTCAGTTTCAAGAACAGACACCAAATCTTCTGGGAAAAGAGCAAAATCCCATGTCGGAGGAGCGGCGATTTCAGGCTGTGGGCTTTTGCCGGAATCGATATTGGAAGTGCTCAGTCGGTACTCGAGGCGGCGGCTGTGTTGACGGCCATCATTGTCGTTCACGAGAGTGGCCACTTCCTCGCCGCTTGTCTACAGGGTATCCATGTAAGTAAATTTGCAATTGGTTTTGGTCCAATTCTTGCAAAATTCAACAAAAACAACGTAGAATACTCCATTAGGGCTTTTCCTCTTGGTGGATTTGTGGGTTTTCCTGATAATGATCCTGATAGCGATATTCCAATGGATGACGAAAATTTGCTTAAAAATAGACCTATATTGGATAGAGTATTAGTGATTTCTGCTGGTGTTATTGCCAATATAATATTCGCATACATAATCATCTTAATCCAAGTCTCGTCGGTTGGTTTGCCGGTTCAAGAGCCCTTTCCCGGCGTGCTTGTGCCTGAGGTTCGAACCCTTTCGGCTGCTTCTCGAGATGGGCTGCTTCCAGGGGATGTGATTCTTGCTGTTAATGGAAATGAACTGCCAAAATTGGGTCCCACAGCCGTTTCTGAGCTTGTTGAGACAATTAAAAGAAGCCCCAATAGAACTGTTCTTCTCAAAGTTGAAAGAGGGCAACAGGATCTTGAAATTGGGGTCACTCCAGATGAGAGTTTTGATGGAACAGGGCGAATTGGGGTTCAGCTTTCGCCTAATGTAAAGATTTCAAAACTTGTAGCGAAGAATTTCTTGGAGGCTTTTAGCTATTCTAGAAAGGAATTTTTGGGTCTGTCATATAATGTTCTTGATAGCTTGAAACAAACTGTTCTGAATTTCTCTCAGTCAGCCAGTAAGGTTTCTGGTCCTGTGGCCATTATTGCTGTTGGTGCAGAAGTTGCAAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAATATTAACCTTGCTGTTATAAACCTTCTTCCCTTGCCTGCTTTGGATGGCGGCTCGTTGGCCTTGATCCTCATTGAGGCTGCTAGGGGCGGGAGGAAGTTGCCTCTAGAACTTGAGCAGCGAATCATGTCGTCTGGGATCATGGTTGTTGTACTTCTTGGGCTGTTCCTCATCGTCCGAGACACGTTAAACCTTGAATTTATAAAGGACTTGTTATGA

mRNA sequence

ATGCTATTAAATCTCTCTTCCTCTTCTTATTCAGTCATTAAATTTGCCAATTCGAAGTCACCCTTCTCAGATTTTTCAGTCAAGTACAGAACCCACTTCTCTAAATCCCAATTTTCTTCTTCATTATGTCATTCTTTAACGTCATCGGAGCTCAGTTTCAAGAACAGACACCAAATCTTCTGGGAAAAGAGCAAAATCCCATGTCGGAGGAGCGGCGATTTCAGGCTGTGGGCTTTTGCCGGAATCGATATTGGAAGTGCTCAGTCGGTACTCGAGGCGGCGGCTGTGTTGACGGCCATCATTGTCGTTCACGAGAGTGGCCACTTCCTCGCCGCTTGTCTACAGGGTATCCATGTAAGTAAATTTGCAATTGGTTTTGGTCCAATTCTTGCAAAATTCAACAAAAACAACGTAGAATACTCCATTAGGGCTTTTCCTCTTGGTGGATTTGTGGGTTTTCCTGATAATGATCCTGATAGCGATATTCCAATGGATGACGAAAATTTGCTTAAAAATAGACCTATATTGGATAGAGTATTAGTGATTTCTGCTGGTGTTATTGCCAATATAATATTCGCATACATAATCATCTTAATCCAAGTCTCGTCGGTTGGTTTGCCGGTTCAAGAGCCCTTTCCCGGCGTGCTTGTGCCTGAGGTTCGAACCCTTTCGGCTGCTTCTCGAGATGGGCTGCTTCCAGGGGATGTGATTCTTGCTGTTAATGGAAATGAACTGCCAAAATTGGGTCCCACAGCCGTTTCTGAGCTTGTTGAGACAATTAAAAGAAGCCCCAATAGAACTGTTCTTCTCAAAGTTGAAAGAGGGCAACAGGATCTTGAAATTGGGGTCACTCCAGATGAGAGTTTTGATGGAACAGGGCGAATTGGGGTTCAGCTTTCGCCTAATGTAAAGATTTCAAAACTTGTAGCGAAGAATTTCTTGGAGGCTTTTAGCTATTCTAGAAAGGAATTTTTGGGTCTGTCATATAATGTTCTTGATAGCTTGAAACAAACTGTTCTGAATTTCTCTCAGTCAGCCAGTAAGGTTTCTGGTCCTGTGGCCATTATTGCTGTTGGTGCAGAAGTTGCAAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAATATTAACCTTGCTGTTATAAACCTTCTTCCCTTGCCTGCTTTGGATGGCGGCTCGTTGGCCTTGATCCTCATTGAGGCTGCTAGGGGCGGGAGGAAGTTGCCTCTAGAACTTGAGCAGCGAATCATGTCGTCTGGGATCATGGTTGTTGTACTTCTTGGGCTGTTCCTCATCGTCCGAGACACGTTAAACCTTGAATTTATAAAGGACTTGTTATGA

Coding sequence (CDS)

ATGCTATTAAATCTCTCTTCCTCTTCTTATTCAGTCATTAAATTTGCCAATTCGAAGTCACCCTTCTCAGATTTTTCAGTCAAGTACAGAACCCACTTCTCTAAATCCCAATTTTCTTCTTCATTATGTCATTCTTTAACGTCATCGGAGCTCAGTTTCAAGAACAGACACCAAATCTTCTGGGAAAAGAGCAAAATCCCATGTCGGAGGAGCGGCGATTTCAGGCTGTGGGCTTTTGCCGGAATCGATATTGGAAGTGCTCAGTCGGTACTCGAGGCGGCGGCTGTGTTGACGGCCATCATTGTCGTTCACGAGAGTGGCCACTTCCTCGCCGCTTGTCTACAGGGTATCCATGTAAGTAAATTTGCAATTGGTTTTGGTCCAATTCTTGCAAAATTCAACAAAAACAACGTAGAATACTCCATTAGGGCTTTTCCTCTTGGTGGATTTGTGGGTTTTCCTGATAATGATCCTGATAGCGATATTCCAATGGATGACGAAAATTTGCTTAAAAATAGACCTATATTGGATAGAGTATTAGTGATTTCTGCTGGTGTTATTGCCAATATAATATTCGCATACATAATCATCTTAATCCAAGTCTCGTCGGTTGGTTTGCCGGTTCAAGAGCCCTTTCCCGGCGTGCTTGTGCCTGAGGTTCGAACCCTTTCGGCTGCTTCTCGAGATGGGCTGCTTCCAGGGGATGTGATTCTTGCTGTTAATGGAAATGAACTGCCAAAATTGGGTCCCACAGCCGTTTCTGAGCTTGTTGAGACAATTAAAAGAAGCCCCAATAGAACTGTTCTTCTCAAAGTTGAAAGAGGGCAACAGGATCTTGAAATTGGGGTCACTCCAGATGAGAGTTTTGATGGAACAGGGCGAATTGGGGTTCAGCTTTCGCCTAATGTAAAGATTTCAAAACTTGTAGCGAAGAATTTCTTGGAGGCTTTTAGCTATTCTAGAAAGGAATTTTTGGGTCTGTCATATAATGTTCTTGATAGCTTGAAACAAACTGTTCTGAATTTCTCTCAGTCAGCCAGTAAGGTTTCTGGTCCTGTGGCCATTATTGCTGTTGGTGCAGAAGTTGCAAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAATATTAACCTTGCTGTTATAAACCTTCTTCCCTTGCCTGCTTTGGATGGCGGCTCGTTGGCCTTGATCCTCATTGAGGCTGCTAGGGGCGGGAGGAAGTTGCCTCTAGAACTTGAGCAGCGAATCATGTCGTCTGGGATCATGGTTGTTGTACTTCTTGGGCTGTTCCTCATCGTCCGAGACACGTTAAACCTTGAATTTATAAAGGACTTGTTATGA

Protein sequence

MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRHQIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIMSSGIMVVVLLGLFLIVRDTLNLEFIKDLL

Homology

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BLAST of CmaCh20G006110 vs. ExPASy Swiss-Prot
Match: O23053 (Probable membrane metalloprotease ARASP2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ARASP2 PE=2 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 6.5e-161
Identity = 308/452 (68.14%), Postives = 370/452 (81.86%), Query Frame = 0


Query: 1   MLLNLSSS--SYSVIKF-ANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRH 60
           MLLN+SSS  S+ +  F ++  +P S+F  K +TH  KS  S+   HSL      +  ++
Sbjct: 1   MLLNISSSPISHRIPHFLSDFNNPTSNFPPKSKTHLPKSNLSTLSNHSL------YGTKN 60

Query: 61  QIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGI 120
           + F++  + P  R+      A    D GS +SVLEA+AVLTAIIVVHE+GHFLAA LQGI
Sbjct: 61  RAFYKNKRNPYNRT-----QALGRFDFGSLESVLEASAVLTAIIVVHETGHFLAASLQGI 120

Query: 121 HVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILD 180
            VSKFAIGFGPILAKFN NNVEYS+RAFPLGGFVGFPDNDPDSDIP+DD NLLKNRPILD
Sbjct: 121 RVSKFAIGFGPILAKFNSNNVEYSLRAFPLGGFVGFPDNDPDSDIPVDDRNLLKNRPILD 180

Query: 181 RVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVI 240
           RV+V+SAG++AN+IFAY II  QV SVGLPVQE FPGVLVP+V++ SAASRDGLLPGDVI
Sbjct: 181 RVIVVSAGIVANVIFAYAIIFTQVVSVGLPVQESFPGVLVPDVKSFSAASRDGLLPGDVI 240

Query: 241 LAVNGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGV 300
           LAV+G EL   G  +VS++V+ +KR+P   VLL++ERG++  EI +TPD+SFDGTG+IGV
Sbjct: 241 LAVDGTELSNSGSDSVSKVVDVVKRNPEHNVLLRIERGKESFEIRITPDKSFDGTGKIGV 300

Query: 301 QLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIA 360
           QLSPNV+  K+  KN  E FS++ +EF GLSYNVLDSLKQT LNFSQ+ASKV+GPVAIIA
Sbjct: 301 QLSPNVRFGKVRPKNIPETFSFAGREFFGLSYNVLDSLKQTFLNFSQTASKVAGPVAIIA 360

Query: 361 VGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQ 420
           VGAEVARSN DGLYQFAA+LN+NLAVINLLPLPALDGG+LALIL+EA RGGRKLPLE+EQ
Sbjct: 361 VGAEVARSNADGLYQFAALLNLNLAVINLLPLPALDGGTLALILLEAVRGGRKLPLEVEQ 420

Query: 421 RIMSSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
            IMSSGIM+V+ LGLFLIV+DTLNL+FIK++L
Sbjct: 421 GIMSSGIMLVLFLGLFLIVKDTLNLDFIKEML 441

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BLAST of CmaCh20G006110 vs. ExPASy Swiss-Prot
Match: O80885 (Membrane metalloprotease ARASP, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ARASP PE=2 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 1.9e-160
Identity = 309/452 (68.36%), Postives = 366/452 (80.97%), Query Frame = 0


Query: 1   MLLNLSSSSYSVIK---FANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRH 60
           MLLN+SSS  S       +N  +P S F  + +TH SKS F          S  S KNR 
Sbjct: 1   MLLNISSSPISHRNPHFLSNFNNPISYFPRRSKTHLSKSHFFPKF---TPLSNQSLKNR- 60

Query: 61  QIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGI 120
            + +   + P     DFR  A +GID+GS +SVLEA AVLT IIVVHESGHFLAA LQGI
Sbjct: 61  -VLFGNKRYPDGERFDFRSRAISGIDLGSFESVLEAIAVLTTIIVVHESGHFLAASLQGI 120

Query: 121 HVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILD 180
           HVSKFAIGFGPILAKF+ NNVEYS+RAFPLGGFVGFPDNDPDS+IP+DDENLLKNRP LD
Sbjct: 121 HVSKFAIGFGPILAKFDYNNVEYSLRAFPLGGFVGFPDNDPDSEIPIDDENLLKNRPTLD 180

Query: 181 RVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVI 240
           R +V+SAG+IAN+IFAY II +QV SVGLPVQE FPGVLVPEV+T SAASRDGLL GDVI
Sbjct: 181 RSIVVSAGIIANVIFAYAIIFVQVLSVGLPVQEAFPGVLVPEVKTFSAASRDGLLSGDVI 240

Query: 241 LAVNGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGV 300
           LAV+G EL K GP AVS++V+ +KR+P   V+ ++ERG +D +I VTPD++FDGTG+IGV
Sbjct: 241 LAVDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFDIRVTPDKNFDGTGKIGV 300

Query: 301 QLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIA 360
           QLSPNV+I+K+  +N  E F +  +EF+GLS NVLD LKQT  NFSQ+ASKV+GPVAIIA
Sbjct: 301 QLSPNVRITKVRPRNIPETFRFVGREFMGLSSNVLDGLKQTFFNFSQTASKVAGPVAIIA 360

Query: 361 VGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQ 420
           VGAEVARSNIDGLYQFAA+LNINLAVINLLPLPALDGG+LALIL+EA RGG+KLP+E+EQ
Sbjct: 361 VGAEVARSNIDGLYQFAALLNINLAVINLLPLPALDGGTLALILLEAVRGGKKLPVEVEQ 420

Query: 421 RIMSSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
            IMSSGIM+V+ LGLFLIV+DTL+L+FIK++L
Sbjct: 421 GIMSSGIMLVIFLGLFLIVKDTLSLDFIKEML 447

[Close]

BLAST of CmaCh20G006110 vs. ExPASy Swiss-Prot
Match: P73714 (Putative zinc metalloprotease slr1821 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1821 PE=3 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.2e-91
Identity = 179/364 (49.18%), Postives = 256/364 (70.33%), Query Frame = 0


Query: 86  SAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAF 145
           S  + L A  VL  +I VHE GHF AA LQGIHV++FA+GFGP L K+     EYSIRA 
Sbjct: 2   SVLAALAAIGVLAVLIAVHELGHFAAARLQGIHVTRFALGFGPPLLKYQGAETEYSIRAI 61

Query: 146 PLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVLVISAGVIANIIFAYIIILIQVSSVG 205
           PLGG+V FPD+DPDS+IP DD NLLKNRPILDR +VISAGVIAN++FAY +++ QVS++G
Sbjct: 62  PLGGYVAFPDDDPDSEIPADDPNLLKNRPILDRAIVISAGVIANLVFAYFLLIGQVSTIG 121

Query: 206 LPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGPTAVSELVETIKRSPN 265
              Q   PG+++P+V + SAA   G+ PGD++L++ GN LP   P A ++ ++ ++RSP+
Sbjct: 122 F--QNIQPGLVIPQVDSASAAQVAGMEPGDIVLSLQGNTLPGF-PDATTQFIDIVRRSPS 181

Query: 266 RTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLSPNVKISKLVAKNFLEAFSYSRKEFL 325
             + ++V+RG++   + +TP    +G G+IGV L PNV+  +  A N LEA +YS + F 
Sbjct: 182 VPITVEVQRGEETKTLTITPTPDAEGKGKIGVALLPNVETKR--ASNPLEALTYSAEAFE 241

Query: 326 GLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVIN 385
            +         Q + NF+ +AS+V+GPV I+  GA +ARS+   L+QF A+++INLAVIN
Sbjct: 242 RIVKLTTQGFWQLISNFADNASQVAGPVKIVEYGANIARSDASNLFQFGALISINLAVIN 301

Query: 386 LLPLPALDGGSLALILIEAARGGRKLPLELEQRIMSSGIMVVVLLGLFLIVRDTLNLEFI 445
           +LPLPALDGG L  +LIE    G+ LP + +  +M +G+++++ LG+FLIVRDTLNL F+
Sbjct: 302 ILPLPALDGGQLVFLLIEGLL-GKPLPEKFQMGVMQTGLVLLLSLGVFLIVRDTLNLTFV 359

Query: 446 KDLL 450
           ++ L
Sbjct: 362 QEFL 359

[Close]

BLAST of CmaCh20G006110 vs. ExPASy Swiss-Prot
Match: Q8YQ64 (Putative zinc metalloprotease all3971 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=all3971 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.0e-81
Identity = 176/358 (49.16%), Postives = 244/358 (68.16%), Query Frame = 0


Query: 89  SVLEAAAVLTAIIVVHESGHFLAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLG 148
           SVL A AVL  +I+VHE GHF+AA  QGIHV++F++GFGP+L K+     EY+IRAFPLG
Sbjct: 2   SVLAAIAVLAVLILVHELGHFVAARSQGIHVNRFSLGFGPVLWKYQGAETEYAIRAFPLG 61

Query: 149 GFVGFPDNDPDSDIPMDDENLLKNRPILDRVLVISAGVIANIIFAYIIILIQVSSVGLPV 208
           GFVGFPD+DPDSDIP +D NLL+NRPILDR +VISAGVIAN+IFAY+++L QV  VG+  
Sbjct: 62  GFVGFPDDDPDSDIPPNDPNLLRNRPILDRAIVISAGVIANLIFAYMLLLAQVGFVGIG- 121

Query: 209 QEPFPGV----LVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGPTAVSELVETIKRSP 268
           Q   PGV    L PEV   + A+  GL PGDVIL+ N  E        +  L + IK SP
Sbjct: 122 QASQPGVSIQQLAPEVS--AVATNAGLKPGDVILSANQKEF-GTSLQEIEALRDIIKNSP 181

Query: 269 NRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLSPNVKISKLVAKNFLEAFSYSRKEF 328
            +++ L V RG + L + V P+    G G IG+ L+PN K+ +    +  +AFS    EF
Sbjct: 182 GKSIQLTVARGDERLSVNVIPEAKPAG-GSIGIGLAPNGKVERRPV-SLSKAFSVGASEF 241

Query: 329 LGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVI 388
             +         Q V NF ++AS+V+GP+ I+ +GA +A+++   L+ FAA+++INLAVI
Sbjct: 242 QRIVVMTFKGFGQLVTNFGETASQVAGPIKIVEIGANIAQNDTGSLFFFAALISINLAVI 301

Query: 389 NLLPLPALDGGSLALILIEAARGGRKLPLELEQRIMSSGIMVVVLLGLFLIVRDTLNL 443
           N+LPLPALDGG LA +LIE  R G+ LP  +++ +M +G+++++ LG+FLIV++T  L
Sbjct: 302 NILPLPALDGGQLAFLLIEGLR-GKPLPNRIQEGVMQTGLVLLLGLGIFLIVKETTQL 352

[Close]

BLAST of CmaCh20G006110 vs. ExPASy Swiss-Prot
Match: O31754 (Regulator of sigma-W protease RasP OS=Bacillus subtilis (strain 168) OX=224308 GN=rasP PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.8e-30
Identity = 123/435 (28.28%), Postives = 189/435 (43.45%), Query Frame = 0


Query: 89  SVLEAAAVLTAIIVVHESGHFLAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLG 148
           +V+    +   ++  HE GH L A   GI   +FAIGFGP +  F KN   Y+IR  P+G
Sbjct: 5   TVIAFIIIFGTLVFFHELGHLLLAQRAGILCREFAIGFGPKIFSFKKNETVYTIRLLPVG 64

Query: 149 GFVGFPDNDPD-----------------------------------------SDIPMD-- 208
           GFV     DP+                                         +D+  D  
Sbjct: 65  GFVRMAGEDPEMIEVKPGYTVGLLFNKEDQVEKVIINQKEKYPDALVIEVETADLEHDMK 124

Query: 209 ---------DE-------------------------NLLKNRPILDRVLVISAGVIANII 268
                    DE                             ++P+  R+  I+AG I N I
Sbjct: 125 ITGYEQGKEDELSSFTVSETSFFIVDGEEVQIAPYNRQFGSKPVWQRIKAIAAGPIMNFI 184

Query: 269 FAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGPT 328
            AY+I+++     G+P  EP  G L    R    A+  GL  GD I ++NG ++      
Sbjct: 185 LAYVILVMLGLIQGVPSNEPMLGQLTDNGR----AAEAGLKEGDYIQSINGEKM-----R 244

Query: 329 AVSELVETIKRSPNRTVLLKVERGQQDLEIGVTP----DESFDGTGRIGVQLSPNVKISK 388
           + +++V  +K +P + + + V+R  + L I VTP    DE+    GR G   +P      
Sbjct: 245 SWTDIVSAVKENPEKEMDVAVKRDNKTLHISVTPEAVKDENKKTIGRFG-SYAP------ 304

Query: 389 LVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGAEVARSNI 443
              K  L A +Y     + ++  +L +L + V         +SGPV I  +  +VA++ I
Sbjct: 305 -TEKGVLSAVAYGATSTVDVTKAILTNLSKLVTG-QFKLDMLSGPVGIYDMTDQVAKTGI 364

[Close]

BLAST of CmaCh20G006110 vs. ExPASy TrEMBL
Match: A0A6J1JFQ4 (probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484092 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.5e-240
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 0


Query: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRHQIF 60
           MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRHQIF
Sbjct: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRHQIF 60

Query: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120
           WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS
Sbjct: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120

Query: 121 KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180
           KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL
Sbjct: 121 KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180

Query: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240
           VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV
Sbjct: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240

Query: 241 NGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300
           NGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS
Sbjct: 241 NGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300

Query: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360
           PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA
Sbjct: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360

Query: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420
           EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM
Sbjct: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420

Query: 421 SSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
           SSGIMVVVLLGLFLIVRDTLNLEFIKDLL
Sbjct: 421 SSGIMVVVLLGLFLIVRDTLNLEFIKDLL 449

[Close]

BLAST of CmaCh20G006110 vs. ExPASy TrEMBL
Match: A0A6J1FWS5 (probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448141 PE=3 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 2.7e-234
Identity = 438/449 (97.55%), Postives = 443/449 (98.66%), Query Frame = 0


Query: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRHQIF 60
           MLLNLSSSSYS+ KFANS+SPFSDFSVKYRTHFSKSQ SSSLC+SLTSSELSFKNRH IF
Sbjct: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRHHIF 60

Query: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120
           WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS
Sbjct: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120

Query: 121 KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180
           KFAIGFGP+LAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL
Sbjct: 121 KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180

Query: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240
           VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV
Sbjct: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240

Query: 241 NGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300
           NGNELPKLG TAVSELVE IKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS
Sbjct: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300

Query: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360
           PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLN SQSASKVSGPVAIIAVGA
Sbjct: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGA 360

Query: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420
           EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM
Sbjct: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420

Query: 421 SSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
           SSGIMVVVLLG+FLIVRDTLNLEFIKDLL
Sbjct: 421 SSGIMVVVLLGMFLIVRDTLNLEFIKDLL 449

[Close]

BLAST of CmaCh20G006110 vs. ExPASy TrEMBL
Match: A0A0A0LT84 (PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G417840 PE=3 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.4e-221
Identity = 419/456 (91.89%), Postives = 432/456 (94.74%), Query Frame = 0


Query: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSK-------SQFSSSLCHSLTSSELSF 60
           MLLNL SSS+SVIK AN KSPFSDFSVK RTHFSK       S  SSSL HS  SSELSF
Sbjct: 1   MLLNLPSSSFSVIKLANLKSPFSDFSVKSRTHFSKSLSSSSSSSSSSSLWHSPPSSELSF 60

Query: 61  KNRHQIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAAC 120
           K+RHQIFW+K+KI CRR+GDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAAC
Sbjct: 61  KDRHQIFWDKTKIRCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAAC 120

Query: 121 LQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNR 180
           LQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIP+DDENLLKNR
Sbjct: 121 LQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPVDDENLLKNR 180

Query: 181 PILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLP 240
           PILDRVLVISAGVIANIIFAYIIIL+QVSSVGLPVQEPFPGVLVPEV TLSAASRDGLLP
Sbjct: 181 PILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLLP 240

Query: 241 GDVILAVNGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTG 300
           GDVILAVNGNELPKLG TAVS+LVE IKRSPNRTVLLKVERG QDLEIGVTPD+SFDGTG
Sbjct: 241 GDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGTG 300

Query: 301 RIGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPV 360
           RIGVQLSPNVKISK+VAKNFLEAF+YSRKEFLGLSYNVLDSLKQT LNFSQSASKVSGPV
Sbjct: 301 RIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPV 360

Query: 361 AIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPL 420
           AIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEA RGGRKLPL
Sbjct: 361 AIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLPL 420

Query: 421 ELEQRIMSSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
           ELEQRIMSSG+M VVLLGLFLI+RDTLNLEFIKDLL
Sbjct: 421 ELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 456

[Close]

BLAST of CmaCh20G006110 vs. ExPASy TrEMBL
Match: A0A5D3E082 (Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3695G00250 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 2.9e-220
Identity = 417/457 (91.25%), Postives = 431/457 (94.31%), Query Frame = 0


Query: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSK--------SQFSSSLCHSLTSSELS 60
           MLLNL SSS+SVIK AN KSPFSDFSVK RTHFSK        S  SSSL HS  SSELS
Sbjct: 1   MLLNLPSSSFSVIKLANLKSPFSDFSVKSRTHFSKSLSSSSSSSSSSSSLWHSPPSSELS 60

Query: 61  FKNRHQIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FKNRHQIFW+K+KI CRR+GDFRLWAFAG+DIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKNRHQIFWDKTKIRCRRNGDFRLWAFAGMDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKN 180
           CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDP+SDIP+DDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRVLVISAGVIANIIFAYIIIL+QVSSVGLPVQEPFPGVLVPEV TLSAASRDGLL
Sbjct: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELP LG TAVS+LVE IKRSPNRTVLLKVERG QDLEIGVTPD+SFDGT
Sbjct: 241 PGDVILAVNGNELPNLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGT 300

Query: 301 GRIGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGP 360
           GRIGVQLSPNVKISK+VAKNFLEAF+YSRKEFLGLSYNVLDSLKQT LNFSQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEA RGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420

Query: 421 LELEQRIMSSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
           LELEQRIMSSG+M VVLLGLFLI+RDTLNLEFIKDLL
Sbjct: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 457

[Close]

BLAST of CmaCh20G006110 vs. ExPASy TrEMBL
Match: A0A1S3C376 (probable membrane metalloprotease ARASP2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103495966 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 2.9e-220
Identity = 417/457 (91.25%), Postives = 431/457 (94.31%), Query Frame = 0


Query: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSK--------SQFSSSLCHSLTSSELS 60
           MLLNL SSS+SVIK AN KSPFSDFSVK RTHFSK        S  SSSL HS  SSELS
Sbjct: 1   MLLNLPSSSFSVIKLANLKSPFSDFSVKSRTHFSKSLSSSSSSSSSSSSLWHSPPSSELS 60

Query: 61  FKNRHQIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FKNRHQIFW+K+KI CRR+GDFRLWAFAG+DIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKNRHQIFWDKTKIRCRRNGDFRLWAFAGMDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKN 180
           CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDP+SDIP+DDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRVLVISAGVIANIIFAYIIIL+QVSSVGLPVQEPFPGVLVPEV TLSAASRDGLL
Sbjct: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELP LG TAVS+LVE IKRSPNRTVLLKVERG QDLEIGVTPD+SFDGT
Sbjct: 241 PGDVILAVNGNELPNLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGT 300

Query: 301 GRIGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGP 360
           GRIGVQLSPNVKISK+VAKNFLEAF+YSRKEFLGLSYNVLDSLKQT LNFSQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEA RGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420

Query: 421 LELEQRIMSSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
           LELEQRIMSSG+M VVLLGLFLI+RDTLNLEFIKDLL
Sbjct: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 457

[Close]

BLAST of CmaCh20G006110 vs. NCBI nr
Match: XP_022986309.1 (probable membrane metalloprotease ARASP2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 841.6 bits (2173), Expect = 3.0e-240
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 0


Query: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRHQIF 60
           MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRHQIF
Sbjct: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRHQIF 60

Query: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120
           WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS
Sbjct: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120

Query: 121 KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180
           KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL
Sbjct: 121 KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180

Query: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240
           VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV
Sbjct: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240

Query: 241 NGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300
           NGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS
Sbjct: 241 NGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300

Query: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360
           PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA
Sbjct: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360

Query: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420
           EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM
Sbjct: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420

Query: 421 SSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
           SSGIMVVVLLGLFLIVRDTLNLEFIKDLL
Sbjct: 421 SSGIMVVVLLGLFLIVRDTLNLEFIKDLL 449

[Close]

BLAST of CmaCh20G006110 vs. NCBI nr
Match: XP_023512495.1 (probable membrane metalloprotease ARASP2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512496.1 probable membrane metalloprotease ARASP2, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 826.6 bits (2134), Expect = 1.0e-235
Identity = 443/449 (98.66%), Postives = 444/449 (98.89%), Query Frame = 0


Query: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRHQIF 60
           MLLNLSSSSYS+IKFANSKSPFSD SVKYRTHFSKSQ SSSLCHSLTSSELSFKNRHQIF
Sbjct: 1   MLLNLSSSSYSLIKFANSKSPFSDVSVKYRTHFSKSQSSSSLCHSLTSSELSFKNRHQIF 60

Query: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120
           WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS
Sbjct: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120

Query: 121 KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180
           KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL
Sbjct: 121 KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180

Query: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240
           VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV
Sbjct: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240

Query: 241 NGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300
           NGNELPKLG TAVSELVE IKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS
Sbjct: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300

Query: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360
           PNVKISKLVAKN LEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA
Sbjct: 301 PNVKISKLVAKNSLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360

Query: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420
           EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM
Sbjct: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420

Query: 421 SSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
           SSGIMVVVLLGLFLIVRDTLNLEFIKDLL
Sbjct: 421 SSGIMVVVLLGLFLIVRDTLNLEFIKDLL 449

[Close]

BLAST of CmaCh20G006110 vs. NCBI nr
Match: KAG6570888.1 (Membrane metalloprotease ARASP, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 824.7 bits (2129), Expect = 3.8e-235
Identity = 440/449 (98.00%), Postives = 445/449 (99.11%), Query Frame = 0


Query: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRHQIF 60
           MLLNLSSSSYS+IKFANS+SPF+DFSVKYRTHFSKSQ SSSLCHSLTSSELSFKNRHQIF
Sbjct: 1   MLLNLSSSSYSLIKFANSRSPFTDFSVKYRTHFSKSQSSSSLCHSLTSSELSFKNRHQIF 60

Query: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120
           WEKSKIP RRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS
Sbjct: 61  WEKSKIPFRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120

Query: 121 KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180
           KFAIGFGP+LAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL
Sbjct: 121 KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180

Query: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240
           VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV
Sbjct: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240

Query: 241 NGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300
           NGNELPKLG TAVSELVE IKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS
Sbjct: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300

Query: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360
           PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA
Sbjct: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360

Query: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420
           EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM
Sbjct: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420

Query: 421 SSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
           SSGIMVVVLLG+FLIVRDTLNLEFIKDLL
Sbjct: 421 SSGIMVVVLLGMFLIVRDTLNLEFIKDLL 449

[Close]

BLAST of CmaCh20G006110 vs. NCBI nr
Match: XP_022943353.1 (probable membrane metalloprotease ARASP2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 820.8 bits (2119), Expect = 5.6e-234
Identity = 438/449 (97.55%), Postives = 443/449 (98.66%), Query Frame = 0


Query: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRHQIF 60
           MLLNLSSSSYS+ KFANS+SPFSDFSVKYRTHFSKSQ SSSLC+SLTSSELSFKNRH IF
Sbjct: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRHHIF 60

Query: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120
           WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS
Sbjct: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120

Query: 121 KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180
           KFAIGFGP+LAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL
Sbjct: 121 KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180

Query: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240
           VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV
Sbjct: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240

Query: 241 NGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300
           NGNELPKLG TAVSELVE IKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS
Sbjct: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300

Query: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360
           PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLN SQSASKVSGPVAIIAVGA
Sbjct: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGA 360

Query: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420
           EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM
Sbjct: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420

Query: 421 SSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
           SSGIMVVVLLG+FLIVRDTLNLEFIKDLL
Sbjct: 421 SSGIMVVVLLGMFLIVRDTLNLEFIKDLL 449

[Close]

BLAST of CmaCh20G006110 vs. NCBI nr
Match: XP_038902635.1 (probable membrane metalloprotease ARASP2, chloroplastic [Benincasa hispida])

HSP 1 Score: 782.7 bits (2020), Expect = 1.7e-222
Identity = 417/455 (91.65%), Postives = 434/455 (95.38%), Query Frame = 0


Query: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSK------SQFSSSLCHSLTSSELSFK 60
           ML+NLSSSS+SVIK A  KSPFSDFSVK RTHFSK      S  SSS CHS   S+LSFK
Sbjct: 1   MLVNLSSSSFSVIKLAKLKSPFSDFSVKSRTHFSKSLSSSSSSSSSSFCHSFPLSDLSFK 60

Query: 61  NRHQIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACL 120
           NRHQIFW+K+KIPCRR+GDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACL
Sbjct: 61  NRHQIFWDKTKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACL 120

Query: 121 QGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRP 180
           QGIHVSKFAIGFGP+LAKFNKNNVEYSIRAFPLGGFVGFPDNDP+SDIP+DDENLLKNRP
Sbjct: 121 QGIHVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKNRP 180

Query: 181 ILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPG 240
           ILDRVLVISAGVIANIIFAYIIIL+QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPG
Sbjct: 181 ILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPG 240

Query: 241 DVILAVNGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGR 300
           DVILAVNGNELPKLG TAVS+LVE IKRSPNRTVLLKVERG+QDLEIGVTPDESFDGTGR
Sbjct: 241 DVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGTGR 300

Query: 301 IGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVA 360
           IGVQLSPNVKISK+VAKNFLEAF+YSRKEFLGLSY+VLDSLKQT LNFSQSASKVSGPVA
Sbjct: 301 IGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYSVLDSLKQTFLNFSQSASKVSGPVA 360

Query: 361 IIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLE 420
           IIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEA RGGRKLPLE
Sbjct: 361 IIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLPLE 420

Query: 421 LEQRIMSSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
           LEQRIMSSG+M VVLLGLFLI+RDTLNLEFIKDLL
Sbjct: 421 LEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 455

[Close]

BLAST of CmaCh20G006110 vs. TAIR 10
Match: AT1G05140.1 (Peptidase M50 family protein )

HSP 1 Score: 568.5 bits (1464), Expect = 4.6e-162
Identity = 308/452 (68.14%), Postives = 370/452 (81.86%), Query Frame = 0


Query: 1   MLLNLSSS--SYSVIKF-ANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRH 60
           MLLN+SSS  S+ +  F ++  +P S+F  K +TH  KS  S+   HSL      +  ++
Sbjct: 1   MLLNISSSPISHRIPHFLSDFNNPTSNFPPKSKTHLPKSNLSTLSNHSL------YGTKN 60

Query: 61  QIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGI 120
           + F++  + P  R+      A    D GS +SVLEA+AVLTAIIVVHE+GHFLAA LQGI
Sbjct: 61  RAFYKNKRNPYNRT-----QALGRFDFGSLESVLEASAVLTAIIVVHETGHFLAASLQGI 120

Query: 121 HVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILD 180
            VSKFAIGFGPILAKFN NNVEYS+RAFPLGGFVGFPDNDPDSDIP+DD NLLKNRPILD
Sbjct: 121 RVSKFAIGFGPILAKFNSNNVEYSLRAFPLGGFVGFPDNDPDSDIPVDDRNLLKNRPILD 180

Query: 181 RVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVI 240
           RV+V+SAG++AN+IFAY II  QV SVGLPVQE FPGVLVP+V++ SAASRDGLLPGDVI
Sbjct: 181 RVIVVSAGIVANVIFAYAIIFTQVVSVGLPVQESFPGVLVPDVKSFSAASRDGLLPGDVI 240

Query: 241 LAVNGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGV 300
           LAV+G EL   G  +VS++V+ +KR+P   VLL++ERG++  EI +TPD+SFDGTG+IGV
Sbjct: 241 LAVDGTELSNSGSDSVSKVVDVVKRNPEHNVLLRIERGKESFEIRITPDKSFDGTGKIGV 300

Query: 301 QLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIA 360
           QLSPNV+  K+  KN  E FS++ +EF GLSYNVLDSLKQT LNFSQ+ASKV+GPVAIIA
Sbjct: 301 QLSPNVRFGKVRPKNIPETFSFAGREFFGLSYNVLDSLKQTFLNFSQTASKVAGPVAIIA 360

Query: 361 VGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQ 420
           VGAEVARSN DGLYQFAA+LN+NLAVINLLPLPALDGG+LALIL+EA RGGRKLPLE+EQ
Sbjct: 361 VGAEVARSNADGLYQFAALLNLNLAVINLLPLPALDGGTLALILLEAVRGGRKLPLEVEQ 420

Query: 421 RIMSSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
            IMSSGIM+V+ LGLFLIV+DTLNL+FIK++L
Sbjct: 421 GIMSSGIMLVLFLGLFLIVKDTLNLDFIKEML 441

[Close]

BLAST of CmaCh20G006110 vs. TAIR 10
Match: AT2G32480.1 (ARABIDOPSIS SERIN PROTEASE )

HSP 1 Score: 567.0 bits (1460), Expect = 1.3e-161
Identity = 309/452 (68.36%), Postives = 366/452 (80.97%), Query Frame = 0


Query: 1   MLLNLSSSSYSVIK---FANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRH 60
           MLLN+SSS  S       +N  +P S F  + +TH SKS F          S  S KNR 
Sbjct: 1   MLLNISSSPISHRNPHFLSNFNNPISYFPRRSKTHLSKSHFFPKF---TPLSNQSLKNR- 60

Query: 61  QIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGI 120
            + +   + P     DFR  A +GID+GS +SVLEA AVLT IIVVHESGHFLAA LQGI
Sbjct: 61  -VLFGNKRYPDGERFDFRSRAISGIDLGSFESVLEAIAVLTTIIVVHESGHFLAASLQGI 120

Query: 121 HVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILD 180
           HVSKFAIGFGPILAKF+ NNVEYS+RAFPLGGFVGFPDNDPDS+IP+DDENLLKNRP LD
Sbjct: 121 HVSKFAIGFGPILAKFDYNNVEYSLRAFPLGGFVGFPDNDPDSEIPIDDENLLKNRPTLD 180

Query: 181 RVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVI 240
           R +V+SAG+IAN+IFAY II +QV SVGLPVQE FPGVLVPEV+T SAASRDGLL GDVI
Sbjct: 181 RSIVVSAGIIANVIFAYAIIFVQVLSVGLPVQEAFPGVLVPEVKTFSAASRDGLLSGDVI 240

Query: 241 LAVNGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGV 300
           LAV+G EL K GP AVS++V+ +KR+P   V+ ++ERG +D +I VTPD++FDGTG+IGV
Sbjct: 241 LAVDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFDIRVTPDKNFDGTGKIGV 300

Query: 301 QLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIA 360
           QLSPNV+I+K+  +N  E F +  +EF+GLS NVLD LKQT  NFSQ+ASKV+GPVAIIA
Sbjct: 301 QLSPNVRITKVRPRNIPETFRFVGREFMGLSSNVLDGLKQTFFNFSQTASKVAGPVAIIA 360

Query: 361 VGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQ 420
           VGAEVARSNIDGLYQFAA+LNINLAVINLLPLPALDGG+LALIL+EA RGG+KLP+E+EQ
Sbjct: 361 VGAEVARSNIDGLYQFAALLNINLAVINLLPLPALDGGTLALILLEAVRGGKKLPVEVEQ 420

Query: 421 RIMSSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
            IMSSGIM+V+ LGLFLIV+DTL+L+FIK++L
Sbjct: 421 GIMSSGIMLVIFLGLFLIVKDTLSLDFIKEML 447

[Close]

BLAST of CmaCh20G006110 vs. TAIR 10
Match: AT2G32480.2 (ARABIDOPSIS SERIN PROTEASE )

HSP 1 Score: 511.5 bits (1316), Expect = 6.7e-145
Identity = 289/452 (63.94%), Postives = 341/452 (75.44%), Query Frame = 0


Query: 1   MLLNLSSSSYSVIK---FANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRH 60
           MLLN+SSS  S       +N  +P S F  + +TH SKS F          S  S KNR 
Sbjct: 1   MLLNISSSPISHRNPHFLSNFNNPISYFPRRSKTHLSKSHFFPKF---TPLSNQSLKNR- 60

Query: 61  QIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGI 120
            + +   + P     DFR  A +GID+GS +SVLEA AVLT IIVVHESGHFLAA LQGI
Sbjct: 61  -VLFGNKRYPDGERFDFRSRAISGIDLGSFESVLEAIAVLTTIIVVHESGHFLAASLQGI 120

Query: 121 HVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILD 180
           HVSKFAIGFGPILAKF+ NNVEYS+RAFPLGGFVGFPDNDPDS+IP+DDENLLKNRP LD
Sbjct: 121 HVSKFAIGFGPILAKFDYNNVEYSLRAFPLGGFVGFPDNDPDSEIPIDDENLLKNRPTLD 180

Query: 181 RVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVI 240
           R +V+SAG+IAN+IFAY II +QV SVGLPVQE FPGVLVPEV+T SAASRDGLL GDVI
Sbjct: 181 RSIVVSAGIIANVIFAYAIIFVQVLSVGLPVQEAFPGVLVPEVKTFSAASRDGLLSGDVI 240

Query: 241 LAVNGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGV 300
           LAV+G EL K GP AVS++V+ +KR+P   V+ ++ERG +D +I VTPD++FDGTG+IGV
Sbjct: 241 LAVDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFDIRVTPDKNFDGTGKIGV 300

Query: 301 QLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIA 360
           QLSPNV+I+K                                     +ASKV+GPVAIIA
Sbjct: 301 QLSPNVRITK-------------------------------------TASKVAGPVAIIA 360

Query: 361 VGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQ 420
           VGAEVARSNIDGLYQFAA+LNINLAVINLLPLPALDGG+LALIL+EA RGG+KLP+E+EQ
Sbjct: 361 VGAEVARSNIDGLYQFAALLNINLAVINLLPLPALDGGTLALILLEAVRGGKKLPVEVEQ 410

Query: 421 RIMSSGIMVVVLLGLFLIVRDTLNLEFIKDLL 450
            IMSSGIM+V+ LGLFLIV+DTL+L+FIK++L
Sbjct: 421 GIMSSGIMLVIFLGLFLIVKDTLSLDFIKEML 410

The following BLAST results are available for this feature:

BLAST of CmaCh20G006110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23053	6.5e-161	68.14	Probable membrane metalloprotease ARASP2, chloroplastic OS=Arabidopsis thaliana ...	[more]
O80885	1.9e-160	68.36	Membrane metalloprotease ARASP, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
P73714	3.2e-91	49.18	Putative zinc metalloprotease slr1821 OS=Synechocystis sp. (strain PCC 6803 / Ka...	[more]
Q8YQ64	1.0e-81	49.16	Putative zinc metalloprotease all3971 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82...	[more]
O31754	1.8e-30	28.28	Regulator of sigma-W protease RasP OS=Bacillus subtilis (strain 168) OX=224308 G...	[more]

BLAST of CmaCh20G006110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JFQ4	1.5e-240	100.00	probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita maxima OX=3...	[more]
A0A6J1FWS5	2.7e-234	97.55	probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita moschata OX...	[more]
A0A0A0LT84	3.4e-221	91.89	PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G417840 PE=3 SV...	[more]
A0A5D3E082	2.9e-220	91.25	Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A1S3C376	2.9e-220	91.25	probable membrane metalloprotease ARASP2, chloroplastic OS=Cucumis melo OX=3656 ...	[more]

BLAST of CmaCh20G006110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022986309.1	3.0e-240	100.00	probable membrane metalloprotease ARASP2, chloroplastic [Cucurbita maxima]	[more]
XP_023512495.1	1.0e-235	98.66	probable membrane metalloprotease ARASP2, chloroplastic isoform X1 [Cucurbita pe...	[more]
KAG6570888.1	3.8e-235	98.00	Membrane metalloprotease ARASP, chloroplastic, partial [Cucurbita argyrosperma s...	[more]
XP_022943353.1	5.6e-234	97.55	probable membrane metalloprotease ARASP2, chloroplastic [Cucurbita moschata]	[more]
XP_038902635.1	1.7e-222	91.65	probable membrane metalloprotease ARASP2, chloroplastic [Benincasa hispida]	[more]

BLAST of CmaCh20G006110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05140.1	4.6e-162	68.14	Peptidase M50 family protein	[more]
AT2G32480.1	1.3e-161	68.36	ARABIDOPSIS SERIN PROTEASE	[more]
AT2G32480.2	6.7e-145	63.94	ARABIDOPSIS SERIN PROTEASE	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001478	PDZ domain	SMART	SM00228	pdz_new	coord: 202..276 e-value: 3.6E-9 score: 46.5
IPR001478	PDZ domain	PROSITE	PS50106	PDZ	coord: 204..275 score: 10.9182
IPR008915	Peptidase M50	PFAM	PF02163	Peptidase_M50	coord: 94..430 e-value: 1.7E-59 score: 201.1
IPR036034	PDZ superfamily	GENE3D	2.30.42.10		coord: 204..300 e-value: 9.1E-16 score: 60.0
IPR036034	PDZ superfamily	SUPERFAMILY	50156	PDZ domain-like	coord: 202..287
IPR041489	PDZ domain 6	PFAM	PF17820	PDZ_6	coord: 217..274 e-value: 4.5E-8 score: 32.9
IPR004387	Peptidase M50, putative membrane-associated zinc metallopeptidase	PANTHER	PTHR42837	REGULATOR OF SIGMA-E PROTEASE RSEP	coord: 22..449
None	No IPR available	PANTHER	PTHR42837:SF4	MEMBRANE METALLOPROTEASE ARASP2, CHLOROPLASTIC-RELATED	coord: 22..449
None	No IPR available	CDD	cd06163	S2P-M50_PDZ_RseP-like	coord: 90..441 e-value: 1.7303E-39 score: 138.315
None	No IPR available	CDD	cd00989	PDZ_metalloprotease	coord: 202..285 e-value: 4.42662E-14 score: 65.3327

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh20G006110.1	CmaCh20G006110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0005515	protein binding