CmaCh20G005570 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G005570
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionindole-3-acetic acid-amido synthetase GH3.10
LocationCma_Chr20: 2700455 .. 2703901 (+)
RNA-Seq ExpressionCmaCh20G005570
SyntenyCmaCh20G005570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCATCGGTTTAATTATCTTGTTTTTTTCCAAGTACTATAACGTTCTTTTAGGCCTTTTTAACCCACAACAACAACGCCATACCTCCCAATACAGAGATATACTTGATTGAGTGCTCGTGCTCGTGCTCGTCCTCCTGCTGCTGCGGCTGCGGGCGTGTTCAAGGCGGTGAGGCGCGCACCATGTTTGAAGAAGTAGAAGACATGGTTGATTGGTTCGAGCGAGTGTCGTCAGAAGCAGGCGTGGCTCAGTCGCGCACACTCCGCGAGATTCTGCGCCAGAATTGTGGCGTGGAGTATCTAAACCAATGGCTTGGAGATGTCAATATCCATCAAATTCCTGATGACTTTGCATTGGAATCCCTCTTCACTTCCTTAGTCCCCCTTTCTTCCCACGCTGATTTCGAGCCTTTTCTTCAACGAATCGCCGATGGTGACTCTTCCCCTTTGCTTACTCAACAACCCATTCAAACTCTCTCTTTAAGGTACCCTTCTTCAACATTTTCCCTTACATTTTATGAATTCCCTCTTTATTACAAATGATATCATTACGCCGCTGCAGTTCTGGCACCACCGAAGGCCGACAAAAGTACGTGCCCTTCACTCCACATAGTGCGCAGACTACCCTTCTCATCTTTCGTATAGCTGCTGCTTATAGATCCAGGTACCAATGCAATATGGTCAGAATCTTCATTTTTTTGAGTTACCCTTTTCTTGCAAGACATTAAAGAATGCCTTAATGACGACTAGGGTTTATCCCACGAGGGAAGGAGGCCGGATTTTGGAGTTCATTTACAGTAGTAAACAGACCAAAACCAAAGGAGGGATAACCACCGGCACAGCCACAACCCATTACTACGCCAGTGAGCAATTCAAAATCAAGCAGCTCAAAACCAAATCCTTCACTTGCAGCCCACAAGAAGTCATTTCCGGCGGTGATTACAAGCAATCCACTTACTGTCATCTCCTTCTCGGGCTGTTCTTCTCCGACCAAGTCGAGTTTGTCACCTCAACTTTCGCCTACACCATAGTCCAAGCCTTCAACGAATTGGAAGATTGTTGGCCTCAAATCTGTCACGACATTACCCACGCTACACTCTGCTCTAGAATTACTCTTCCTGAAATCCGTAAGGCTGTTTTGAACATCATTAGTCCGAACCCAATTCTTGGGGCGAAAATTCAAGCTGTTTGTGAAGAGCTTGAGAGGCAGGATTGGTTGGATTTGGTTCCGAAGCTATGGTCGAATTGTAAGTATGTTTACTCCATAATGACGGGGTCTATGCAGCCGTATTTGAAGAAATTGAGACGGTATGCGGGGGGGTTGCCGTTGGTGAGTGGGGATTATGGATCCACGGAGAGTTGGATTGGGATTAATGTTGATCCTTGTCTGCCGCCTGAAAATGTTACTTTTGCCGTTGTACCCACTTTCTCTTATTTTGAGTTCATCCCACTTTTCAGACAACATCAACAACATGATTGTGGCAGTTCCTCCGTTGCTGCCTTTGATGATTTCTTGGAAGGCCAACCCCTTCCCTTGTCTCAAGTCAAGATTGGACAGCAGTACGAACTTGTTCTCACCACTTTCACAGGTTAGTTCATTAATTATCAACACAAATCTAATCAAACGCCTTGCTTTGCTACTTCCTTGTCATTATGTCCACCTCAACTATTAGAACGAGAAGATTAGTGCTAGGAAGAGATTTTCAATCAAATTCACTTTCCTTAGAATCCTACATATAACTCTGTGTCTAGATCTGATACTATCCTTAACGATCCGACTTTTCTATGTAACGACTCTAACGGTTATGGGTAAGAAAAGTCGTTACAATTAGTATCATGATTTGGTAATCATGGATCAATGATTTGATTAGTCCTATACGGCAAGTTGAGAGAAATTTCCAACCTTCCTTATTTGGCTAAAAAATTTCCAACTTTTCTTGTCAACATGATGTTTACATCTCGCAAAGGTAAAATTTTGGGCAAAGATTTGTTATTGAAATTTAGTGGGTGAATATCAATAAAGCCTAAAAAATGTGAAAACTTTTTTACTATAGTTTACATCACATCAAATATAGAGCATTTGATAGTAAATATGTAACTACTCAAGCCTACTGCTACTTGATATTATTTTGATTTTCTCTTTCGAGCTTCCGCTCATTTAATGGTAAATATGTTCACATTTTTTACTAAAGTTTTTTGTCACAAAAAATATAGAGCATATAGATCATATGATTAACATGTAATAGCTTAAGCTTGCAGTTAGCAAATATTGTACTCAGTGTGTTTTTCATTTAAAGCTTCCCTCAAGGTTTTTAAGATGTGATGAGGTGATGCTAGGAATAGGTTTTCATATACTTTTGTTCTCGATCAATTTAGATATAGCTCAAACTGATGATTATGAAATATATGTTTGATCTTCGACCGAAAGGGGTTAATGGGTTTTGGTATTGGTCGGGATTGGCAGGACTATACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCAGGGTTTCACAACAAGACACCTAAACTCAACTTTATATGCCGAAGAAAGCTTGTATTAACCGTAAACATTGACAAGAACACCGAGAAGGATCTCCAATTGGTGGTGGAAAGAGGGTCCCAGCTGCTGGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACCAGCCATGCCGACTTAACCAACCAACCAGGCCACTATGTCATCTTCTGGGAAGTCAAAGGCAATGTGGACGACGCAGTTCTTGGACGCTGCTGCTCGGAGATGGACGCTGCTTTCGTGGACTACGGCTACGTGGTCTCCAGGCGGTCGAACACCATCGGACCTCTTGAGCTAAGGATTGTGGAAAGAGGCAGTTTCAACAAGATCATGGAACATTATATTGGTAATGGAGCTGCGCTTAGCCAATTCAAGACCCCCAGATGCACTACCAATCAGGTTCTTCTTACCATTCTCAATCTCTCCACCGTCAAAGCCTTTCATAGCACTGCTTATGGCTAAGTTTGGTTATTAGTATTGTTAGTATTTCCCAATTTGGAATCAGTTTCGGATCATTTCATTCTAACCAAATTAGAGTGTAACTTACTCTTAGTCAAACACTTACTTATCATCGCATGTAACGAACCAAAGCGAATAATATTTGTTAGTAGTGGGTTTCAGTTATTACAATTAGTATAAGAGATAGACACCGAGCTGTCATATCAATTGGAGAAGAAAACAAATCATTCCTTTAAAGATTTGTTATAGAGCGAGGAAACTTCTCCCTAATAGATGCTATCGTAGTACCTTGAGAGTGAATAAAGTGTCGGTCGTCGGCGTTGTCTCGTTGTTTTTGCTTTTATCATCATAAAGCTGCCTCAACTCTTCTTCCACTGCTAGCTCTTAATGGGTTTGCTTTTGAGTTCTACTCTAATCCTCCATTTGTGCATTATTTACTGCTACACCC

mRNA sequence

TTCCATCGGTTTAATTATCTTGTTTTTTTCCAAGTACTATAACGTTCTTTTAGGCCTTTTTAACCCACAACAACAACGCCATACCTCCCAATACAGAGATATACTTGATTGAGTGCTCGTGCTCGTGCTCGTCCTCCTGCTGCTGCGGCTGCGGGCGTGTTCAAGGCGGTGAGGCGCGCACCATGTTTGAAGAAGTAGAAGACATGGTTGATTGGTTCGAGCGAGTGTCGTCAGAAGCAGGCGTGGCTCAGTCGCGCACACTCCGCGAGATTCTGCGCCAGAATTGTGGCGTGGAGTATCTAAACCAATGGCTTGGAGATGTCAATATCCATCAAATTCCTGATGACTTTGCATTGGAATCCCTCTTCACTTCCTTAGTCCCCCTTTCTTCCCACGCTGATTTCGAGCCTTTTCTTCAACGAATCGCCGATGGTGACTCTTCCCCTTTGCTTACTCAACAACCCATTCAAACTCTCTCTTTAAGTTCTGGCACCACCGAAGGCCGACAAAAGTACGTGCCCTTCACTCCACATAGTGCGCAGACTACCCTTCTCATCTTTCGTATAGCTGCTGCTTATAGATCCAGGGTTTATCCCACGAGGGAAGGAGGCCGGATTTTGGAGTTCATTTACAGTAGTAAACAGACCAAAACCAAAGGAGGGATAACCACCGGCACAGCCACAACCCATTACTACGCCAGTGAGCAATTCAAAATCAAGCAGCTCAAAACCAAATCCTTCACTTGCAGCCCACAAGAAGTCATTTCCGGCGGTGATTACAAGCAATCCACTTACTGTCATCTCCTTCTCGGGCTGTTCTTCTCCGACCAAGTCGAGTTTGTCACCTCAACTTTCGCCTACACCATAGTCCAAGCCTTCAACGAATTGGAAGATTGTTGGCCTCAAATCTGTCACGACATTACCCACGCTACACTCTGCTCTAGAATTACTCTTCCTGAAATCCGTAAGGCTGTTTTGAACATCATTAGTCCGAACCCAATTCTTGGGGCGAAAATTCAAGCTGTTTGTGAAGAGCTTGAGAGGCAGGATTGGTTGGATTTGGTTCCGAAGCTATGGTCGAATTGTAAGTATGTTTACTCCATAATGACGGGGTCTATGCAGCCGTATTTGAAGAAATTGAGACGGTATGCGGGGGGGTTGCCGTTGGTGAGTGGGGATTATGGATCCACGGAGAGTTGGATTGGGATTAATGTTGATCCTTGTCTGCCGCCTGAAAATGTTACTTTTGCCGTTGTACCCACTTTCTCTTATTTTGAGTTCATCCCACTTTTCAGACAACATCAACAACATGATTGTGGCAGTTCCTCCGTTGCTGCCTTTGATGATTTCTTGGAAGGCCAACCCCTTCCCTTGTCTCAAGTCAAGATTGGACAGCAGTACGAACTTGTTCTCACCACTTTCACAGGACTATACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCAGGGTTTCACAACAAGACACCTAAACTCAACTTTATATGCCGAAGAAAGCTTGTATTAACCGTAAACATTGACAAGAACACCGAGAAGGATCTCCAATTGGTGGTGGAAAGAGGGTCCCAGCTGCTGGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACCAGCCATGCCGACTTAACCAACCAACCAGGCCACTATGTCATCTTCTGGGAAGTCAAAGGCAATGTGGACGACGCAGTTCTTGGACGCTGCTGCTCGGAGATGGACGCTGCTTTCGTGGACTACGGCTACGTGGTCTCCAGGCGGTCGAACACCATCGGACCTCTTGAGCTAAGGATTGTGGAAAGAGGCAGTTTCAACAAGATCATGGAACATTATATTGGTAATGGAGCTGCGCTTAGCCAATTCAAGACCCCCAGATGCACTACCAATCAGGTTCTTCTTACCATTCTCAATCTCTCCACCGTCAAAGCCTTTCATAGCACTGCTTATGGCTAAGTTTGGTTATTAGTATTGTTAGTATTTCCCAATTTGGAATCAGTTTCGGATCATTTCATTCTAACCAAATTAGAGTGTAACTTACTCTTAGTCAAACACTTACTTATCATCGCATGTAACGAACCAAAGCGAATAATATTTGTTAGTAGTGGGTTTCAGTTATTACAATTAGTATAAGAGATAGACACCGAGCTGTCATATCAATTGGAGAAGAAAACAAATCATTCCTTTAAAGATTTGTTATAGAGCGAGGAAACTTCTCCCTAATAGATGCTATCGTAGTACCTTGAGAGTGAATAAAGTGTCGGTCGTCGGCGTTGTCTCGTTGTTTTTGCTTTTATCATCATAAAGCTGCCTCAACTCTTCTTCCACTGCTAGCTCTTAATGGGTTTGCTTTTGAGTTCTACTCTAATCCTCCATTTGTGCATTATTTACTGCTACACCC

Coding sequence (CDS)

ATGTTTGAAGAAGTAGAAGACATGGTTGATTGGTTCGAGCGAGTGTCGTCAGAAGCAGGCGTGGCTCAGTCGCGCACACTCCGCGAGATTCTGCGCCAGAATTGTGGCGTGGAGTATCTAAACCAATGGCTTGGAGATGTCAATATCCATCAAATTCCTGATGACTTTGCATTGGAATCCCTCTTCACTTCCTTAGTCCCCCTTTCTTCCCACGCTGATTTCGAGCCTTTTCTTCAACGAATCGCCGATGGTGACTCTTCCCCTTTGCTTACTCAACAACCCATTCAAACTCTCTCTTTAAGTTCTGGCACCACCGAAGGCCGACAAAAGTACGTGCCCTTCACTCCACATAGTGCGCAGACTACCCTTCTCATCTTTCGTATAGCTGCTGCTTATAGATCCAGGGTTTATCCCACGAGGGAAGGAGGCCGGATTTTGGAGTTCATTTACAGTAGTAAACAGACCAAAACCAAAGGAGGGATAACCACCGGCACAGCCACAACCCATTACTACGCCAGTGAGCAATTCAAAATCAAGCAGCTCAAAACCAAATCCTTCACTTGCAGCCCACAAGAAGTCATTTCCGGCGGTGATTACAAGCAATCCACTTACTGTCATCTCCTTCTCGGGCTGTTCTTCTCCGACCAAGTCGAGTTTGTCACCTCAACTTTCGCCTACACCATAGTCCAAGCCTTCAACGAATTGGAAGATTGTTGGCCTCAAATCTGTCACGACATTACCCACGCTACACTCTGCTCTAGAATTACTCTTCCTGAAATCCGTAAGGCTGTTTTGAACATCATTAGTCCGAACCCAATTCTTGGGGCGAAAATTCAAGCTGTTTGTGAAGAGCTTGAGAGGCAGGATTGGTTGGATTTGGTTCCGAAGCTATGGTCGAATTGTAAGTATGTTTACTCCATAATGACGGGGTCTATGCAGCCGTATTTGAAGAAATTGAGACGGTATGCGGGGGGGTTGCCGTTGGTGAGTGGGGATTATGGATCCACGGAGAGTTGGATTGGGATTAATGTTGATCCTTGTCTGCCGCCTGAAAATGTTACTTTTGCCGTTGTACCCACTTTCTCTTATTTTGAGTTCATCCCACTTTTCAGACAACATCAACAACATGATTGTGGCAGTTCCTCCGTTGCTGCCTTTGATGATTTCTTGGAAGGCCAACCCCTTCCCTTGTCTCAAGTCAAGATTGGACAGCAGTACGAACTTGTTCTCACCACTTTCACAGGACTATACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCAGGGTTTCACAACAAGACACCTAAACTCAACTTTATATGCCGAAGAAAGCTTGTATTAACCGTAAACATTGACAAGAACACCGAGAAGGATCTCCAATTGGTGGTGGAAAGAGGGTCCCAGCTGCTGGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACCAGCCATGCCGACTTAACCAACCAACCAGGCCACTATGTCATCTTCTGGGAAGTCAAAGGCAATGTGGACGACGCAGTTCTTGGACGCTGCTGCTCGGAGATGGACGCTGCTTTCGTGGACTACGGCTACGTGGTCTCCAGGCGGTCGAACACCATCGGACCTCTTGAGCTAAGGATTGTGGAAAGAGGCAGTTTCAACAAGATCATGGAACATTATATTGGTAATGGAGCTGCGCTTAGCCAATTCAAGACCCCCAGATGCACTACCAATCAGGTTCTTCTTACCATTCTCAATCTCTCCACCGTCAAAGCCTTTCATAGCACTGCTTATGGCTAA

Protein sequence

MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNCKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Homology
BLAST of CmaCh20G005570 vs. ExPASy Swiss-Prot
Match: Q9ZNS2 (Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=GH3.10 PE=2 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 2.5e-237
Identity = 398/588 (67.69%), Postives = 470/588 (79.93%), Query Frame = 0

Query: 6   EDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           +D++ WFE VS  A   QS TLR IL  N GVEYL +WLG V++ ++ DD+ LE+LFTSL
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLETLFTSL 68

Query: 66  VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           VP+ SHAD +P++QRIADG++SPLLTQ+PI  LSLSSGTTEGRQKYVPFT HSAQTTL I
Sbjct: 69  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 128

Query: 126 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 185
           FR++AAYRSR YP REGGRILEFIY+ K+ KT GG+T GTATTHYYASE+FK KQ  TKS
Sbjct: 129 FRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKS 188

Query: 186 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 245
           FTCSPQEVISGGD+ Q TYCHLLLGL +S QVEFV S F+YTIVQAF+  E+ W +IC D
Sbjct: 189 FTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICAD 248

Query: 246 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQ-DWLDLVPKLWSNCKYV 305
           I    L SRITLP++RKAVL +I PNP L + I+ +C ELE    W  L+ KLW N K++
Sbjct: 249 IKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFI 308

Query: 306 YSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFSYF 365
            SIMTGSM PYL KLR YAGGLPLVS DYGSTESWIG+NVDP LPPE+V+FAV+PTFSYF
Sbjct: 309 SSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYF 368

Query: 366 EFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDV 425
           EFIPL+R+  Q D     +    DF+E +P+PLSQVK+GQ+YELVLTTFTGLYR RLGDV
Sbjct: 369 EFIPLYRRQNQSD-----ICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRLGDV 428

Query: 426 VEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSH 485
           VEV  FH  TPKL+FI RRKL+LT+NIDKNTEKDLQ VV++ SQLL+  + AE+VDFTSH
Sbjct: 429 VEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDFTSH 488

Query: 486 ADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVER 545
           AD+  +PGHYVI+WE++G  DD  L  CC EMD AFVDYGYVVSRR N+IGPLELR+VER
Sbjct: 489 ADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRVVER 548

Query: 546 GSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 593
           G+F K+ E  +G    L+QFKTPRCTTN V+L ILN ST+K F S+AY
Sbjct: 549 GTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

BLAST of CmaCh20G005570 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 1.1e-168
Identity = 292/589 (49.58%), Postives = 407/589 (69.10%), Query Frame = 0

Query: 6   EDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           E++++ FE ++ +AG  Q  TL++IL +N G EYL QW     ++   D  +    F + 
Sbjct: 14  EEVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQW----GLNGKTDSLS----FKNC 73

Query: 66  VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           +P+ +H D EP++ RIADGD SP+LT +PI T+SLSSGTT+G+ K+VPF     ++T+ I
Sbjct: 74  IPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVPFNEELMESTMQI 133

Query: 126 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 185
           F+ +  +R+R +P    G+ L+FIY SKQ KTKGG+  GTATT+ Y + QFK      ++
Sbjct: 134 FKTSFVFRNREFPV-VNGKALQFIYGSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAMQT 193

Query: 186 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 245
             CSP EVI G D++QS YCHLL GL F D+V+ V+STFA++IV AF   E  W ++  +
Sbjct: 194 PCCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVHAFRNFEQIWQELVTN 253

Query: 246 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNCKYVY 305
           I    L SR+ +P +R A+  ++ P+P L   I   C  L   +W  L+P+L+ N +Y+Y
Sbjct: 254 IREGVLSSRVIVPSMRAAMSKLLKPDPELADTIFNKCSRL--SNWYGLIPELFPNTRYIY 313

Query: 306 SIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFSYFE 365
            IMTGSM+PYLKKLR YAG LPL+S DYGS+E WIG NV+P LPPE VT+AV+P   YFE
Sbjct: 314 GIMTGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPPELVTYAVLPNIGYFE 373

Query: 366 FIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDVV 425
           FIPL                  D LE  P+ L++VK+G++YE+V+T F GLYR RLGDVV
Sbjct: 374 FIPLMENL--------------DGLEPMPVGLTEVKLGEEYEIVVTNFAGLYRYRLGDVV 433

Query: 426 EVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHA 485
           ++ GFHN TP+L FICRR L+L++NIDKNTEKDLQL VE  +++L+D+   E+VDFTSH 
Sbjct: 434 KIKGFHNGTPELQFICRRNLLLSINIDKNTEKDLQLAVEAAAKILSDEK-LEVVDFTSHV 493

Query: 486 DLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVERG 545
           +++  PGHYVIFWE+ G   + +L  CC+ +D +FVD GYV SR+ + IG LELRIV+RG
Sbjct: 494 NVSADPGHYVIFWELNGEASEEILKECCNCLDKSFVDAGYVGSRKVHAIGALELRIVKRG 553

Query: 546 SFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
           +F+KI++H++G GAA+SQFKTPRC   TN  +L IL+ + V+++ STA+
Sbjct: 554 TFHKILDHFVGLGAAVSQFKTPRCVGPTNLSVLQILSSNVVESYFSTAF 576

BLAST of CmaCh20G005570 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 589.3 bits (1518), Expect = 4.7e-167
Identity = 296/589 (50.25%), Postives = 399/589 (67.74%), Query Frame = 0

Query: 6   EDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           E +++ FE ++ +AG  Q  TL++IL QN G EYL  W   +N    P        F + 
Sbjct: 15  EKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLW--GLNGRTDP------QTFKNC 74

Query: 66  VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           VP+ +H D EP++QRIADGD SP+LT +PI+T+SLSSGTT+G+ K+VPF     ++T+ I
Sbjct: 75  VPIVTHNDLEPYIQRIADGDLSPILTGKPIETISLSSGTTQGKPKFVPFNDELMESTMQI 134

Query: 126 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 185
           F+ + A+R+R +P    G+ L+FIYSSKQ KTKGG+  GTATT+ Y + QFK       +
Sbjct: 135 FKTSFAFRNREFPI-GNGKALQFIYSSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAMCT 194

Query: 186 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 245
             CSP EVI G D+ QS YCHLL GL F D+V+ V+STFA++IV AF   E  W  +  D
Sbjct: 195 PCCSPDEVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIVHAFRTFEQVWEALVVD 254

Query: 246 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNCKYVY 305
           I    L SR+T+P IR A+  ++ P+P L   I   C  L   +W  L+P L+ N +Y+Y
Sbjct: 255 IREGVLSSRVTVPSIRLAMSKLLKPDPELADTIYNKCSRL--SNWYGLIPDLFPNTRYIY 314

Query: 306 SIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFSYFE 365
            IMTGSM+PYLKKLR YAG LPL+S DYGS+E W+G+NV+P LPPE VT+AV+P   YFE
Sbjct: 315 GIMTGSMEPYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKLPPELVTYAVLPNIGYFE 374

Query: 366 FIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDVV 425
           FIPL       +  +S            P+ L++VK+G++YE+V T F GLYR RLGDVV
Sbjct: 375 FIPLGGNLNGIEQANS------------PVGLTEVKLGEEYEVVFTNFAGLYRYRLGDVV 434

Query: 426 EVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHA 485
           +V GFHN TP+L F+CR  L+L++NIDKNTEKDLQL VE  ++ L D+   E+VDFTSH 
Sbjct: 435 KVKGFHNGTPELQFVCRSNLLLSINIDKNTEKDLQLAVEAAAKRLVDEK-LEVVDFTSHV 494

Query: 486 DLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVERG 545
           +++  PGHYVIFWE+ G   D +L  CC+ +D +F+D GYV SR+ N IG LELRIV+RG
Sbjct: 495 NVSADPGHYVIFWELSGEATDEMLQDCCNCLDRSFIDAGYVSSRKVNAIGALELRIVKRG 554

Query: 546 SFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
           +F+KI++H++G G A+SQFKTPRC    N  LL IL+ + V+ + STA+
Sbjct: 555 TFHKILDHFVGLGGAVSQFKTPRCVGPKNSSLLQILSSNVVETYVSTAF 579

BLAST of CmaCh20G005570 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 3.5e-162
Identity = 285/591 (48.22%), Postives = 392/591 (66.33%), Query Frame = 0

Query: 6   EDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYL-NQWLGDVNIHQIPDDFALESLFTS 65
           E+ ++ FE ++ +A   Q  TL++IL  N   EYL N  LG     +          + S
Sbjct: 7   EETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGRTDAE---------SYKS 66

Query: 66  LVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLL 125
            +PL  H D EP++QRI DGD+SP++T +PI  LSLSSGTT G+ K++PF     +TTL 
Sbjct: 67  CIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQ 126

Query: 126 IFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTK 185
           I+R + A+R+R YP  + G+ L+F+Y SKQ  TKGGI   TATT+ Y  +++K      +
Sbjct: 127 IYRTSYAFRNREYPIGQ-GKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMKDIQ 186

Query: 186 SFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICH 245
           S  CSP EVI G D+ QS YCHLL GL +S++V  V STFA+++V AF   E+ W  +C 
Sbjct: 187 SQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWEDLCT 246

Query: 246 DITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNCKYV 305
           DI    L  ++T P IR+AV  I+ PNP L   I   C  L   +W  ++P LW N KYV
Sbjct: 247 DIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIGL--SNWYGVIPALWPNAKYV 306

Query: 306 YSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFSYF 365
           Y IMTGSM+PYLKKLR YAG LPL+S DYG++E W+G N+DP +PPE VT+AV+P   YF
Sbjct: 307 YGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQVGYF 366

Query: 366 EFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDV 425
           EFIPL +   +    S+S+     ++E  P+ L++V++G+ YE+V+T F GLYR RLGDV
Sbjct: 367 EFIPLEKPIGEETENSASI----HYIESDPVGLTEVEVGKIYEVVITNFAGLYRYRLGDV 426

Query: 426 VEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSH 485
           V++A FHN TP+L FICRR LVL++NIDKNTEKDLQL VE  S+ L  +   E++DFTS 
Sbjct: 427 VKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVEEASKFLEGEK-LEVMDFTSF 486

Query: 486 ADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVER 545
            + ++ PG YVIFWE+ G+  D VL  C + +D AF+D GY  SR+  TIGPLELRI+ +
Sbjct: 487 VERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLELRILRK 546

Query: 546 GSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAYG 594
           G+F +I++H++  G A+SQFKTPR    +N  +L IL+ +  +++ STAYG
Sbjct: 547 GTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQILSRNVTQSYFSTAYG 580

BLAST of CmaCh20G005570 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 546.6 bits (1407), Expect = 3.5e-154
Identity = 276/599 (46.08%), Postives = 387/599 (64.61%), Query Frame = 0

Query: 1   MFEEVED-----MVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 60
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 60

Query: 61  FALESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 120
              E  F S+VPL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 61  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 120

Query: 121 PHSAQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQ 180
               + TL +FR A A+R+R +P  + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  
Sbjct: 121 DELMENTLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPN 180

Query: 181 FKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNEL 240
           FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V + FA+ +V AF   
Sbjct: 181 FKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTF 240

Query: 241 EDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVP 300
           E  W +I  DI    L +RIT+P +R A+  +++PNP L   I+  C  L   +W  L+P
Sbjct: 241 EQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIP 300

Query: 301 KLWSNCKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTF 360
            L+ N KYVY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI  NV P L PE  TF
Sbjct: 301 ALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATF 360

Query: 361 AVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG 420
           AV+P   YFEF+P+       + G           E +P+ L+QVKIG++YE+V+T + G
Sbjct: 361 AVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQVKIGEEYEVVITNYAG 420

Query: 421 LYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSG 480
           LYR RLGDVV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQL VE  ++ L+++  
Sbjct: 421 LYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK- 480

Query: 481 AELVDFTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIG 540
            E++DF+S+ D++  PGHY IFWE+ G  ++ VL  CC+ +D AF+D GYV SR+  TIG
Sbjct: 481 IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIG 540

Query: 541 PLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
            LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 541 ALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of CmaCh20G005570 vs. ExPASy TrEMBL
Match: A0A6J1J741 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC111484069 PE=3 SV=1)

HSP 1 Score: 1214.5 bits (3141), Expect = 0.0e+00
Identity = 593/593 (100.00%), Postives = 593/593 (100.00%), Query Frame = 0

Query: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN 300
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN 300

Query: 301 CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT 360
           CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT 360

Query: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480
           LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 481 FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540
           FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 594
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of CmaCh20G005570 vs. ExPASy TrEMBL
Match: A0A6J1FRQ1 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LOC111448207 PE=3 SV=1)

HSP 1 Score: 1195.6 bits (3092), Expect = 0.0e+00
Identity = 582/593 (98.15%), Postives = 588/593 (99.16%), Query Frame = 0

Query: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFE+VEDMVDWFERVSSEAGVAQ RTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPI+TLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN 300
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEELERQDWLDLVPKLW N
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300

Query: 301 CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT 360
           CKYVYSIMTGSMQPYLKKLRRYAG LPLVSGDYGSTESWIG+NVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360

Query: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480
           LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 481 FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540
           FTSHADLTNQPGHYVIFWEVKG+V+DAVLG CCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 594
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of CmaCh20G005570 vs. ExPASy TrEMBL
Match: A0A5D3B9T4 (Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold464G00300 PE=3 SV=1)

HSP 1 Score: 1023.8 bits (2646), Expect = 2.8e-295
Identity = 493/590 (83.56%), Postives = 545/590 (92.37%), Query Frame = 0

Query: 3   EEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           EE ED++DWFE+VSSEA VAQSRTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L+S+F
Sbjct: 8   EEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLQSIF 67

Query: 63  TSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TSL+PLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSA TT
Sbjct: 68  TSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAHTT 127

Query: 123 LLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLK 182
           LLIFR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQLK
Sbjct: 128 LLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLK 187

Query: 183 TKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQI 242
           TKSFTCSPQEVISG DYKQSTYCHLLLGL +S++VEFVTSTFAYTIVQAFN+LE+ W +I
Sbjct: 188 TKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEESWEEI 247

Query: 243 CHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNCK 302
            HDI+HATL SRI +PEIRKAVL ++SP P LG KI+ VCEEL+++ WL L+PKLW NCK
Sbjct: 248 IHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIPKLWPNCK 307

Query: 303 YVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFS 362
           YVYSIMTGSMQPYLKKLR Y GGLPLVSGDYGSTESWIG+NVDP LPPENVTFAV+PTFS
Sbjct: 308 YVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFS 367

Query: 363 YFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLG 422
           YFEFIPLF+QH      SS+  A  DFLEGQP+ LS+VKIGQQYELVLTTFTGLYRCRLG
Sbjct: 368 YFEFIPLFKQHA---TTSSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGLYRCRLG 427

Query: 423 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFT 482
           DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGSQL++  SGAELVDFT
Sbjct: 428 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLSGAELVDFT 487

Query: 483 SHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELRIV 542
           S+A+L+NQPGHYVIFWEVKG+V+D VLG+CCSEMDAAFVDYGYVVSR++N+IGPLELRIV
Sbjct: 488 SYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANSIGPLELRIV 547

Query: 543 ERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 593
           ERGSFNKI+E+YIGNGAALSQFKTPRCTTN  LL ILNLST+++F STAY
Sbjct: 548 ERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAY 594

BLAST of CmaCh20G005570 vs. ExPASy TrEMBL
Match: A0A1S3BIL6 (jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490483 PE=3 SV=1)

HSP 1 Score: 1023.8 bits (2646), Expect = 2.8e-295
Identity = 493/590 (83.56%), Postives = 545/590 (92.37%), Query Frame = 0

Query: 3   EEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           EE ED++DWFE+VSSEA VAQSRTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L+S+F
Sbjct: 8   EEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLQSIF 67

Query: 63  TSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TSL+PLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSA TT
Sbjct: 68  TSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAHTT 127

Query: 123 LLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLK 182
           LLIFR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQLK
Sbjct: 128 LLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLK 187

Query: 183 TKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQI 242
           TKSFTCSPQEVISG DYKQSTYCHLLLGL +S++VEFVTSTFAYTIVQAFN+LE+ W +I
Sbjct: 188 TKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEESWEEI 247

Query: 243 CHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNCK 302
            HDI+HATL SRI +PEIRKAVL ++SP P LG KI+ VCEEL+++ WL L+PKLW NCK
Sbjct: 248 IHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIPKLWPNCK 307

Query: 303 YVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFS 362
           YVYSIMTGSMQPYLKKLR Y GGLPLVSGDYGSTESWIG+NVDP LPPENVTFAV+PTFS
Sbjct: 308 YVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFS 367

Query: 363 YFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLG 422
           YFEFIPLF+QH      SS+  A  DFLEGQP+ LS+VKIGQQYELVLTTFTGLYRCRLG
Sbjct: 368 YFEFIPLFKQHA---TTSSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGLYRCRLG 427

Query: 423 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFT 482
           DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGSQL++  SGAELVDFT
Sbjct: 428 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLSGAELVDFT 487

Query: 483 SHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELRIV 542
           S+A+L+NQPGHYVIFWEVKG+V+D VLG+CCSEMDAAFVDYGYVVSR++N+IGPLELRIV
Sbjct: 488 SYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANSIGPLELRIV 547

Query: 543 ERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 593
           ERGSFNKI+E+YIGNGAALSQFKTPRCTTN  LL ILNLST+++F STAY
Sbjct: 548 ERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAY 594

BLAST of CmaCh20G005570 vs. ExPASy TrEMBL
Match: A0A0A0KE57 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G124120 PE=3 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 5.4e-291
Identity = 486/590 (82.37%), Postives = 539/590 (91.36%), Query Frame = 0

Query: 3   EEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           E  ED++ WFE+VSSEA +AQSRTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L S+F
Sbjct: 2   EGEEDIIGWFEQVSSEAALAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLHSIF 61

Query: 63  TSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TS +PLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSAQTT
Sbjct: 62  TSSIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAQTT 121

Query: 123 LLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLK 182
           LLIFR+AAAYRSRVYP R+GG+ILEFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQLK
Sbjct: 122 LLIFRLAAAYRSRVYPIRDGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLK 181

Query: 183 TKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQI 242
           TKSFTCSPQEVI+G DYKQSTYCHLLLGL +S++VEFVTSTFAYTIVQAFN+LE+ W ++
Sbjct: 182 TKSFTCSPQEVITGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEESWEEM 241

Query: 243 CHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNCK 302
            HD++HATL SRI +PEIRKAVL ++SP P LG KI+ VCEEL ++ WL L+PKLW NCK
Sbjct: 242 IHDLSHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELMKEGWLGLIPKLWPNCK 301

Query: 303 YVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFS 362
           YVYSIMTGSMQPYLKKLR Y GGLPLVSGDYGSTESWIG+NVDP LPPENVTFAV+PTFS
Sbjct: 302 YVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFS 361

Query: 363 YFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLG 422
           YFEFIPLF+QH      SS+  A  DFLEGQP+ LS+VKIGQQYELVLTTFTGLYRCRLG
Sbjct: 362 YFEFIPLFKQHA---TTSSADTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGLYRCRLG 421

Query: 423 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFT 482
           DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGSQL++  SGAELVDFT
Sbjct: 422 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLSGAELVDFT 481

Query: 483 SHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELRIV 542
           S+A+L+NQPGHYVIFWE+KG+V+D VLG CCSEMDAAFVDYGYVVSR++N+IGPLELRIV
Sbjct: 482 SYAELSNQPGHYVIFWELKGDVNDKVLGMCCSEMDAAFVDYGYVVSRKANSIGPLELRIV 541

Query: 543 ERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 593
           ERGSFNKI+EHYIGNGAALSQFKTPRCTTN  LL ILNLST+K+F STAY
Sbjct: 542 ERGSFNKILEHYIGNGAALSQFKTPRCTTNHFLLNILNLSTLKSFFSTAY 588

BLAST of CmaCh20G005570 vs. NCBI nr
Match: XP_022986272.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima])

HSP 1 Score: 1214.5 bits (3141), Expect = 0.0e+00
Identity = 593/593 (100.00%), Postives = 593/593 (100.00%), Query Frame = 0

Query: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN 300
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN 300

Query: 301 CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT 360
           CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT 360

Query: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480
           LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 481 FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540
           FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 594
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of CmaCh20G005570 vs. NCBI nr
Match: KAG6570848.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1198.3 bits (3099), Expect = 0.0e+00
Identity = 584/593 (98.48%), Postives = 588/593 (99.16%), Query Frame = 0

Query: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEEVEDMVDWFERVSSEAGVAQ RTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN 300
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEELERQDWLDLVPKLW N
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300

Query: 301 CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT 360
           CKYVYSIMTGSMQPYLKKLRRYAG LPLVSGDYGSTESWIG+NVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360

Query: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480
           LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 481 FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540
           FTSHADLTNQPGHYVIFWEVKG+V+DAVLG CCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 594
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of CmaCh20G005570 vs. NCBI nr
Match: XP_023512179.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1197.2 bits (3096), Expect = 0.0e+00
Identity = 584/593 (98.48%), Postives = 588/593 (99.16%), Query Frame = 0

Query: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEEVEDMVDWFERVSSEAGVAQ RTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN 300
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEELERQDWLDLVPKLW N
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300

Query: 301 CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT 360
           CKYVYSIMTGS+QPYLKKLRRYAG LPLVSGDYGSTESWIG+NVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360

Query: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480
           LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 481 FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540
           FTSHADLTNQPGHYVIFWEVKG+V+DAVLG CCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 594
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of CmaCh20G005570 vs. NCBI nr
Match: XP_022943441.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata])

HSP 1 Score: 1195.6 bits (3092), Expect = 0.0e+00
Identity = 582/593 (98.15%), Postives = 588/593 (99.16%), Query Frame = 0

Query: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFE+VEDMVDWFERVSSEAGVAQ RTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPI+TLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN 300
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEELERQDWLDLVPKLW N
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300

Query: 301 CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT 360
           CKYVYSIMTGSMQPYLKKLRRYAG LPLVSGDYGSTESWIG+NVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360

Query: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480
           LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 481 FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540
           FTSHADLTNQPGHYVIFWEVKG+V+DAVLG CCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 594
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of CmaCh20G005570 vs. NCBI nr
Match: KAG7010696.1 (Indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1189.5 bits (3076), Expect = 0.0e+00
Identity = 584/608 (96.05%), Postives = 588/608 (96.71%), Query Frame = 0

Query: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEEVEDMVDWFERVSSEAGVAQ RTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRS---------------RVYPTREGGRILEFIYSSKQTKTKGGITTGT 180
           TTLLIFRIAAAYRS               RVYPTREGGRILEFIYSSKQTKTKGGITTGT
Sbjct: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180

Query: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240
           ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA
Sbjct: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240

Query: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300
           YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL
Sbjct: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300

Query: 301 ERQDWLDLVPKLWSNCKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVD 360
           ERQDWLDLVPKLW NCKYVYSIMTGSMQPYLKKLRRYAG LPLVSGDYGSTESWIG+NVD
Sbjct: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360

Query: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQ 420
           PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVA FDDFLEGQPLPLSQVKIGQQ
Sbjct: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420

Query: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480
           YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER
Sbjct: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480

Query: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGY 540
           GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKG+V+DAVLG CCSEMDAAFVDYGY
Sbjct: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540

Query: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 594
           VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK
Sbjct: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600

BLAST of CmaCh20G005570 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 822.8 bits (2124), Expect = 1.8e-238
Identity = 398/588 (67.69%), Postives = 470/588 (79.93%), Query Frame = 0

Query: 6   EDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           +D++ WFE VS  A   QS TLR IL  N GVEYL +WLG V++ ++ DD+ LE+LFTSL
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLETLFTSL 68

Query: 66  VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           VP+ SHAD +P++QRIADG++SPLLTQ+PI  LSLSSGTTEGRQKYVPFT HSAQTTL I
Sbjct: 69  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 128

Query: 126 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 185
           FR++AAYRSR YP REGGRILEFIY+ K+ KT GG+T GTATTHYYASE+FK KQ  TKS
Sbjct: 129 FRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKS 188

Query: 186 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 245
           FTCSPQEVISGGD+ Q TYCHLLLGL +S QVEFV S F+YTIVQAF+  E+ W +IC D
Sbjct: 189 FTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICAD 248

Query: 246 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQ-DWLDLVPKLWSNCKYV 305
           I    L SRITLP++RKAVL +I PNP L + I+ +C ELE    W  L+ KLW N K++
Sbjct: 249 IKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFI 308

Query: 306 YSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFSYF 365
            SIMTGSM PYL KLR YAGGLPLVS DYGSTESWIG+NVDP LPPE+V+FAV+PTFSYF
Sbjct: 309 SSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYF 368

Query: 366 EFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDV 425
           EFIPL+R+  Q D     +    DF+E +P+PLSQVK+GQ+YELVLTTFTGLYR RLGDV
Sbjct: 369 EFIPLYRRQNQSD-----ICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRLGDV 428

Query: 426 VEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSH 485
           VEV  FH  TPKL+FI RRKL+LT+NIDKNTEKDLQ VV++ SQLL+  + AE+VDFTSH
Sbjct: 429 VEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDFTSH 488

Query: 486 ADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVER 545
           AD+  +PGHYVI+WE++G  DD  L  CC EMD AFVDYGYVVSRR N+IGPLELR+VER
Sbjct: 489 ADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRVVER 548

Query: 546 GSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 593
           G+F K+ E  +G    L+QFKTPRCTTN V+L ILN ST+K F S+AY
Sbjct: 549 GTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

BLAST of CmaCh20G005570 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 546.6 bits (1407), Expect = 2.5e-155
Identity = 276/599 (46.08%), Postives = 387/599 (64.61%), Query Frame = 0

Query: 1   MFEEVED-----MVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 60
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 60

Query: 61  FALESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 120
              E  F S+VPL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 61  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 120

Query: 121 PHSAQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQ 180
               + TL +FR A A+R+R +P  + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  
Sbjct: 121 DELMENTLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPN 180

Query: 181 FKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNEL 240
           FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V + FA+ +V AF   
Sbjct: 181 FKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTF 240

Query: 241 EDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVP 300
           E  W +I  DI    L +RIT+P +R A+  +++PNP L   I+  C  L   +W  L+P
Sbjct: 241 EQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIP 300

Query: 301 KLWSNCKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTF 360
            L+ N KYVY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI  NV P L PE  TF
Sbjct: 301 ALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATF 360

Query: 361 AVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG 420
           AV+P   YFEF+P+       + G           E +P+ L+QVKIG++YE+V+T + G
Sbjct: 361 AVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQVKIGEEYEVVITNYAG 420

Query: 421 LYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSG 480
           LYR RLGDVV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQL VE  ++ L+++  
Sbjct: 421 LYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK- 480

Query: 481 AELVDFTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIG 540
            E++DF+S+ D++  PGHY IFWE+ G  ++ VL  CC+ +D AF+D GYV SR+  TIG
Sbjct: 481 IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIG 540

Query: 541 PLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
            LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 541 ALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of CmaCh20G005570 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 546.6 bits (1407), Expect = 2.5e-155
Identity = 276/599 (46.08%), Postives = 387/599 (64.61%), Query Frame = 0

Query: 1   MFEEVED-----MVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 60
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 60

Query: 61  FALESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 120
              E  F S+VPL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 61  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 120

Query: 121 PHSAQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQ 180
               + TL +FR A A+R+R +P  + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  
Sbjct: 121 DELMENTLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPN 180

Query: 181 FKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNEL 240
           FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V + FA+ +V AF   
Sbjct: 181 FKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTF 240

Query: 241 EDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVP 300
           E  W +I  DI    L +RIT+P +R A+  +++PNP L   I+  C  L   +W  L+P
Sbjct: 241 EQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIP 300

Query: 301 KLWSNCKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTF 360
            L+ N KYVY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI  NV P L PE  TF
Sbjct: 301 ALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATF 360

Query: 361 AVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG 420
           AV+P   YFEF+P+       + G           E +P+ L+QVKIG++YE+V+T + G
Sbjct: 361 AVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQVKIGEEYEVVITNYAG 420

Query: 421 LYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSG 480
           LYR RLGDVV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQL VE  ++ L+++  
Sbjct: 421 LYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK- 480

Query: 481 AELVDFTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIG 540
            E++DF+S+ D++  PGHY IFWE+ G  ++ VL  CC+ +D AF+D GYV SR+  TIG
Sbjct: 481 IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIG 540

Query: 541 PLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
            LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 541 ALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of CmaCh20G005570 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 546.6 bits (1407), Expect = 2.5e-155
Identity = 276/599 (46.08%), Postives = 387/599 (64.61%), Query Frame = 0

Query: 1   MFEEVED-----MVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 60
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 12  MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 71

Query: 61  FALESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 120
              E  F S+VPL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 72  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 131

Query: 121 PHSAQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQ 180
               + TL +FR A A+R+R +P  + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  
Sbjct: 132 DELMENTLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPN 191

Query: 181 FKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNEL 240
           FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V + FA+ +V AF   
Sbjct: 192 FKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTF 251

Query: 241 EDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVP 300
           E  W +I  DI    L +RIT+P +R A+  +++PNP L   I+  C  L   +W  L+P
Sbjct: 252 EQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIP 311

Query: 301 KLWSNCKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTF 360
            L+ N KYVY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI  NV P L PE  TF
Sbjct: 312 ALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATF 371

Query: 361 AVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG 420
           AV+P   YFEF+P+       + G           E +P+ L+QVKIG++YE+V+T + G
Sbjct: 372 AVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQVKIGEEYEVVITNYAG 431

Query: 421 LYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSG 480
           LYR RLGDVV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQL VE  ++ L+++  
Sbjct: 432 LYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK- 491

Query: 481 AELVDFTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIG 540
            E++DF+S+ D++  PGHY IFWE+ G  ++ VL  CC+ +D AF+D GYV SR+  TIG
Sbjct: 492 IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIG 551

Query: 541 PLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
            LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 552 ALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 586

BLAST of CmaCh20G005570 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.8e-145
Identity = 250/514 (48.64%), Postives = 345/514 (67.12%), Query Frame = 0

Query: 81  IADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRIAAAYRSRVYPTR 140
           + DGD+SP+LT  P+  +SLSSGT++GR K++PFT    + TL +FR A A+R+R +P  
Sbjct: 1   MVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAFRNRDFPID 60

Query: 141 EGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYK 200
           + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  FK       S +CSP EVI   D  
Sbjct: 61  DNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVH 120

Query: 201 QSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEI 260
           Q+ YCHLL G+ F DQV++V + FA+ +V AF   E  W +I  DI    L +RIT+P +
Sbjct: 121 QALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSV 180

Query: 261 RKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNCKYVYSIMTGSMQPYLKKLR 320
           R A+  +++PNP L   I+  C  L   +W  L+P L+ N KYVY IMTGSM+PY+ KLR
Sbjct: 181 RTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLR 240

Query: 321 RYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGS 380
            YAG LPLVS DYGS+E WI  NV P L PE  TFAV+P   YFEF+P+       + G 
Sbjct: 241 HYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLPV------SETGE 300

Query: 381 SSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFI 440
                     E +P+ L+QVKIG++YE+V+T + GLYR RLGDVV+V GF+N TP+L FI
Sbjct: 301 G---------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFI 360

Query: 441 CRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEV 500
           CRR L+L++NIDKNTE+DLQL VE  ++ L+++   E++DF+S+ D++  PGHY IFWE+
Sbjct: 361 CRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYIDVSTDPGHYAIFWEI 420

Query: 501 KGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAA 560
            G  ++ VL  CC+ +D AF+D GYV SR+  TIG LELR+V +G+F KI EH++G G++
Sbjct: 421 SGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSS 480

Query: 561 LSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
             QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 481 AGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZNS22.5e-23767.69Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=... [more]
A0A1J6KGJ91.1e-16849.58Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 ... [more]
A0A314KSQ44.7e-16750.25Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 ... [more]
Q6I5813.5e-16248.22Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=3994... [more]
Q9SKE23.5e-15446.08Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1J7410.0e+00100.00indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1FRQ10.0e+0098.15indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A5D3B9T42.8e-29583.56Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BIL62.8e-29583.56jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0KE575.4e-29182.37Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G124120 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022986272.10.0e+00100.00indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima][more]
KAG6570848.10.0e+0098.48Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023512179.10.0e+0098.48indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo][more]
XP_022943441.10.0e+0098.15indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata][more]
KAG7010696.10.0e+0096.05Indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita argyrosperma subsp. argy... [more]
Match NameE-valueIdentityDescription
AT4G03400.11.8e-23867.69Auxin-responsive GH3 family protein [more]
AT2G46370.12.5e-15546.08Auxin-responsive GH3 family protein [more]
AT2G46370.22.5e-15546.08Auxin-responsive GH3 family protein [more]
AT2G46370.42.5e-15546.08Auxin-responsive GH3 family protein [more]
AT2G46370.31.8e-14548.64Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 11..572
e-value: 6.0E-175
score: 583.1
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 6..592
NoneNo IPR availablePANTHERPTHR31901:SF48INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.10coord: 6..592

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G005570.1CmaCh20G005570.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009416 response to light stimulus
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016881 acid-amino acid ligase activity