CmaCh20G005480 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G005480
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionglucomannan 4-beta-mannosyltransferase 2-like
LocationCma_Chr20: 2660461 .. 2665534 (+)
RNA-Seq ExpressionCmaCh20G005480
SyntenyCmaCh20G005480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACACACGCGCTCTCTGTCTCTCTTTCTCTGCTCCACTGCCTTTGCTACTGTTTATCCCCTTCTTCAATTTCCCCCTAAAATAATACTTTTGGTAGCAGAGGCAGAGGCAGACGCAGAGGCCATTGGGGGGTTGGCCAGGTTGTTAAGAGAGAGAGAGAGAGAGAGAAAGAACAGAGAGATCACAACAATTTTCTACTGCAAAGAAGAGGGAGATCTGAGTTGGAACTTCCCTTTTCTGCTCTGTTTTCCATATTTCCAAGGATTTTTGTTATTTCCCTTCCATTTAAACCATTCCCATTCCCATTTACTTCTTTCCTTCTTTCAAAATCCTTTAAAACCCATCGATCCGTCGGTTTCTTCTTCTTCCTCATTCGCCGCTCTGTTTCAGACAGAGGCCATGGCAGAAGCTTCACAGATTCTCTTACCGGAGTCGTTTCAAGGGGGAAGAGGCGATTTCACTGACCAAATTTGGCTCATATGGGAGCTCATCAAAGCGCCATTGATAGTTCCTGTGCTTAGATTAATGGTTTATATCTCATTGACAATGTCTCTCATGCTTTTCTTTGAAAGGCTTTATATGGGAATTGTCATCATTCTTGTTAAGCTGTTTTGGAAGAAGCCTGAGAAACGTTACAATTATGAACCCATTCAGGATGATTTGGAATTGGGAAGCTCCAATTTCCCTCATGTTCTCATTCAAATCCCTATGTTTAACGAAAGAGAGGTGGGTTCTCCTCAATTTTGCCTCCCCTGTTTTCTCTTACATTTGTTTATCAAATAACAGAGTTCTGAAATCTGCGACTGTTTTGTTTGTTACAGGTTTACAAGATCTCCATTGGAGCTGCTTGTGGACTTTCATGGCCGGCCGACCGCCTCGTCATCCAAGTTCTTGACGATTCAACCGACCCTGTCATTAAGGTACTTAAAAACTCTTCTAATCTCCCGACTCTCATCGTTTTCAATCCCCAAATCACAAAAACCTGATTTCCCAACACGAAACCCACACGATTCTTATCGAATCCAAATCCAATGTGGATTTACACACATTTTTGCAGCAAATGGTGGAACAAGAATGCCTAAGATGGGCAAGCAAAGGGATCAACATAACATACCAAATCAGAGAAACTAGAGGAGGATACAAAGCTGGAGCTCTGAAAGAAGGGCTTAAACGGAGCTACGTTAAACACTGTGAATATGTCGCCATTTTCGACGCCGATTTCAGGCCGGAGCCGGATTATCTCCGGCGGGCCATTCCGTTCTTAGTCCATAACTCAGATATTGCTCTGGTTCAAGCTCGCTGGAGATTCGGTAAGCTGCTTTACCTGTTCTTTTCCATTTTACAGTCAATTCGATTCCGTTTTGGTTCATGCTCTTGTGATTGATTTAGCTGCATTGTTTATTTGTGGAAATTTAACATAAGCGTTGATTAATTCCACTTATGGGTTCGTTTAATTATCAGTGATGCAATTTCCTAGAGTGTCGGTGGTCGAATAATAGTAGTCTTTTAAAAAAGATAGAACCAAATAAATAAATAAAAAAAATAATAATAATTTTCTCCCGTTCTGTAAGTTTTGTTTTTGTCTAATCAACCCCCTTATCGTCGGCGGGGGAAGATTGGATGCAGGAGGGGTATGTGGGGCCCACATTAACACACACACACACACACAAAAGAGCCATCAATCACGGGGTGGTCCCCACATTTTAGAAACCCTCTCTGCCACCATGATCGGGACACATGTTCTACAGAAAGCTCATCACGAGCTGTGGTCCCCACTCTCTGAACAAAAAATTAAATAAAAATAAATATATAAGAAAAATACCTAGCATTTGCTAAAATGTATGATTTATTTCATATAATCTTAGTCCCATTTTTATAGTATAATTGTTAGTTAATTTTTGTATTTTTCAATGAATTTAAATTTAATAAGATTTAGAATATAGTATAATGTAATATGGTTCAGTAGGTAGGACTTTAGACTAATTTAATTTTAATTGTCGCATTAATTACCCTCGATCAATGGCACTTAAATAGCTTTGTCAAAATTTAATTTTATTAATAAAATTAAAAAGCTGTCTTTTTTTGTTTATTTATTTATTTATTTTTACGATTATAGGTCGTTTAATAAATTATGTAAAACGTTATGGCCTTTTTGCCAATTGTCAACATCCTGTCCCCTAAGAATACCAGAACTTGCAGTGTTGAGTGGGGTAGAATTGACATTTCATATTCTGAAAACTTTTTTCTCCGTAGAGTTCGGGGTAGTTTTGTCATTTGGAAAAAAGCTGCCACTACTGTCCACGTGCCTTTTCCACAAAATTCCTTTTTTTTTTTTTTTGCTAAATTAAATTTAATTTGGGTATTTATTTAAAGTGTTTCGTACAATAAACGCCTTACTGGTAGGCCTCGTAATTGCTACCGACACTACCGAAGTTTGTCTTGCTCTGGATGACGTGGCACATTCTTTGCTGTGATCACGTTAGGGGTATAACGGGAATACCGTGAATCAAATTTGCCCAATTAACTAATCAAAAGTTGCCAACTCATTCCCCACATGAAACTTCCTCCATGTGGGTCACACTTGATTACCTGTACATATCCACGTGGCCTGCTTCTTTTTGTAGATGAGTCAGAATATTATTATTATTATTATTATTATTACATTGATAACAATGATTATTCTTCATAGTTACTTGTTGATTTGAGTTGGAAAAGTGTTTTTCAATTCATAATTTCGAAAATTCCCTCTTAATAATCAATTATTTCAATTTTTTTGGCAAAATTATAATTTTTTTATTACACAAAAATTTCTCTTTTATTGTTATTTTTTTTTTTTTTGGCAATAGTGAATGCAGATGAGTGCTTGTTGACCAGAATGCAAGAGATGTCTTTGGATTACCATTTCACAGTGGAGCAAGAAGTTGGGTCTGCTACCCATGCCTTCTTTGGCTTCAATGGTATTCTACTTTATCCCTTATAAACACATTATCATGGTCATTTATATTCACAAACAAGCATATTTTAGTAATGGGTCACGACCATTTTATACAAAGATCATCGTTCATCATATGAATTTGTGTCTACATCTTATTTGATAACCATTTTGTTTTAAGAATTTGACTAAAAATTTGAATGCGCGTGGGATGGTGTGTAGTTTATGAGTATGTGACATGGCGCAGGAACTGCTGGTGTATGGAGAATTGCTGCAATTAATGAGGCAGGTGGGTGGAAGGACAGGACCACAGTGGAAGACATGGACCTTGCGGTTCGAGCTAGTCTTAGAGGCTGGAAATTTGTCTACTTAGGTGACCTGCAGGTGAAAAAAAAATTACCCATCTGAAAAAAAACCACCTTTTCTTGTGGTGTTTCTTGTCGTTGTTGGATTTTCCGACCAAACAATGAAGTTCTTGTCATGTTTCTTGATTACAGGTAAAAAGTGAGCTTCCTAGTACTTTCAAAGCCTTCCGTTTTCAGCAGCACCGATGGTCTTGTGGCCCTGCCAATCTTTTCCGCAAAATGGTCATGGAAATTGTCAGAAACAAGGTCTTACCTTAATCTCTGCTAACATAAATAACCCAAATAGTTTCTTGTTGAATCCATAATTAAAGCTGATAAGTTCTCCATGAACTTTGGTTGTTGTTGTTGCAGAAAGTTAAATTCTGGAAGAAAGTTTATGTGATTTACAGTTTCTTCTTCGTTCGGAAGATCATTGCCCATATGGTTACTTTCTTCTTTTACTGTGTTGTTCTTCCCCTTACCATTTTGGTTCCTGAAGTGTATGTACCAATCTGGGGAGCTGTTTACATTCCTTCAATCATTACCATTTTGAACTCAGTGGGAACTCCAAGGTTTGTTTTTGTGTTTTTTGACAGCCCATTTTTGTGTGTTCTTTTACAACAAAACACCAAAAGTCATCTTTCTCTTGTTGTGCTATGGAAAAAAAATGCAGGTCAATTCACCTTCTGTTCTACTGGATTCTTTTCGAAAACGTGATGTCATTGCACCGCACGAAAGCAACTCTGATTGGTCTGTTCGAAGCGGGTCGAGCCAACGAATGGGTTGTGACTGAAAAACTTGGAGATGCTCTGAAGAACAAGGCAGCTGCTGATGCTGCCAAGCTGAAGGCAAATGCCAAAGTGCCTAAAATAAGGTTGAGGTGCAAGTTGGGAGACAGGTAAAAAAACATCCTCTTGAACAGAACTGAATTATGTTTGGCAATTGTGAAAACTGAGTGTTGTGTTGTTATTTTGTTTGAAATGAGGACAGAATCAACACATTGGAGCTGGGATTTGCAGCGTTCCTGTTCTTGTGTGGTTGCTATGACTTTGTTCATGGGAAGAACTGCTACTTTGTGTACCTATTCCTTCAGACATTCAGCTTTCTGATCACTGGAATTGGCTATGTTGGCACCATCATCCCAAGCTCTTGAGAAGCGAAGACCACTTCCTTTAAAAAAGATTATTCAAATATATATATATATATATATATATGATATATTTAGTAAAGCTGATAATGTTCTACAGAAGGAGTAGCCTGCCTAACTTAGAACAAGAAAGTGGGGTTTCAGGCAATAGTTCAGTTCAGTTCAGTTCAGATAAAAGCCAGCTTCTTGGAAGAACAAGATGAACATGATGAACATGATGAACATGTTGAACATGATGAACAAGAACAGAGAGGTTAGGATTTGAAGAGGAAAAAGGGGAATTGCTATGGGTATTTTCGTCATTTAGATTGTTTTTTTTTTTTTTTTCTTTTTGAGATTGTGAAATACTTTTTGTCATTTAATGGAGTTGGCCCATTTGTTACCTTTTTCCTTAAGAGTTTTGCGAGGGAATAATGGACATGGATTAAAGGATGGACTTGTAATAACAATTGTTTACCATTTCTCAAGCTTTTCCATTTTTTTTGTTTTTTTTTTCCTTTTCTTGGGAGCATAACGTCTTAGTGGCACACCACGACGCAATGTTAAGCCCTAATATCATTTATAACAACTCAAACCCATCGTTAATAGATATTATCTAATTTGACTCGTTACTATCTTTGTCAATTTCAAAATTTTTAAAATGCTTTTACTGG

mRNA sequence

ACACACGCGCTCTCTGTCTCTCTTTCTCTGCTCCACTGCCTTTGCTACTGTTTATCCCCTTCTTCAATTTCCCCCTAAAATAATACTTTTGGTAGCAGAGGCAGAGGCAGACGCAGAGGCCATTGGGGGGTTGGCCAGGTTGTTAAGAGAGAGAGAGAGAGAGAGAAAGAACAGAGAGATCACAACAATTTTCTACTGCAAAGAAGAGGGAGATCTGAGTTGGAACTTCCCTTTTCTGCTCTGTTTTCCATATTTCCAAGGATTTTTGTTATTTCCCTTCCATTTAAACCATTCCCATTCCCATTTACTTCTTTCCTTCTTTCAAAATCCTTTAAAACCCATCGATCCGTCGGTTTCTTCTTCTTCCTCATTCGCCGCTCTGTTTCAGACAGAGGCCATGGCAGAAGCTTCACAGATTCTCTTACCGGAGTCGTTTCAAGGGGGAAGAGGCGATTTCACTGACCAAATTTGGCTCATATGGGAGCTCATCAAAGCGCCATTGATAGTTCCTGTGCTTAGATTAATGGTTTATATCTCATTGACAATGTCTCTCATGCTTTTCTTTGAAAGGCTTTATATGGGAATTGTCATCATTCTTGTTAAGCTGTTTTGGAAGAAGCCTGAGAAACGTTACAATTATGAACCCATTCAGGATGATTTGGAATTGGGAAGCTCCAATTTCCCTCATGTTCTCATTCAAATCCCTATGTTTAACGAAAGAGAGGTTTACAAGATCTCCATTGGAGCTGCTTGTGGACTTTCATGGCCGGCCGACCGCCTCGTCATCCAAGTTCTTGACGATTCAACCGACCCTGTCATTAAGCAAATGGTGGAACAAGAATGCCTAAGATGGGCAAGCAAAGGGATCAACATAACATACCAAATCAGAGAAACTAGAGGAGGATACAAAGCTGGAGCTCTGAAAGAAGGGCTTAAACGGAGCTACGTTAAACACTGTGAATATGTCGCCATTTTCGACGCCGATTTCAGGCCGGAGCCGGATTATCTCCGGCGGGCCATTCCGTTCTTAGTCCATAACTCAGATATTGCTCTGGTTCAAGCTCGCTGGAGATTCGTGAATGCAGATGAGTGCTTGTTGACCAGAATGCAAGAGATGTCTTTGGATTACCATTTCACAGTGGAGCAAGAAGTTGGGTCTGCTACCCATGCCTTCTTTGGCTTCAATGGAACTGCTGGTGTATGGAGAATTGCTGCAATTAATGAGGCAGGTGGGTGGAAGGACAGGACCACAGTGGAAGACATGGACCTTGCGGTTCGAGCTAGTCTTAGAGGCTGGAAATTTGTCTACTTAGGTGACCTGCAGGTAAAAAGTGAGCTTCCTAGTACTTTCAAAGCCTTCCGTTTTCAGCAGCACCGATGGTCTTGTGGCCCTGCCAATCTTTTCCGCAAAATGGTCATGGAAATTGTCAGAAACAAGAAAGTTAAATTCTGGAAGAAAGTTTATGTGATTTACAGTTTCTTCTTCGTTCGGAAGATCATTGCCCATATGGTTACTTTCTTCTTTTACTGTGTTGTTCTTCCCCTTACCATTTTGGTTCCTGAAGTGTATGTACCAATCTGGGGAGCTGTTTACATTCCTTCAATCATTACCATTTTGAACTCAGTGGGAACTCCAAGGTCAATTCACCTTCTGTTCTACTGGATTCTTTTCGAAAACGTGATGTCATTGCACCGCACGAAAGCAACTCTGATTGGTCTGTTCGAAGCGGGTCGAGCCAACGAATGGGTTGTGACTGAAAAACTTGGAGATGCTCTGAAGAACAAGGCAGCTGCTGATGCTGCCAAGCTGAAGGCAAATGCCAAAGTGCCTAAAATAAGGTTGAGGTGCAAGTTGGGAGACAGAATCAACACATTGGAGCTGGGATTTGCAGCGTTCCTGTTCTTGTGTGGTTGCTATGACTTTGTTCATGGGAAGAACTGCTACTTTGTGTACCTATTCCTTCAGACATTCAGCTTTCTGATCACTGGAATTGGCTATGTTGGCACCATCATCCCAAGCTCTTGAGAAGCGAAGACCACTTCCTTTAAAAAAGATTATTCAAATATATATATATATATATATATATGATATATTTAGTAAAGCTGATAATGTTCTACAGAAGGAGTAGCCTGCCTAACTTAGAACAAGAAAGTGGGGTTTCAGGCAATAGTTCAGTTCAGTTCAGTTCAGATAAAAGCCAGCTTCTTGGAAGAACAAGATGAACATGATGAACATGATGAACATGTTGAACATGATGAACAAGAACAGAGAGGTTAGGATTTGAAGAGGAAAAAGGGGAATTGCTATGGGTATTTTCGTCATTTAGATTGTTTTTTTTTTTTTTTTCTTTTTGAGATTGTGAAATACTTTTTGTCATTTAATGGAGTTGGCCCATTTGTTACCTTTTTCCTTAAGAGTTTTGCGAGGGAATAATGGACATGGATTAAAGGATGGACTTGTAATAACAATTGTTTACCATTTCTCAAGCTTTTCCATTTTTTTTGTTTTTTTTTTCCTTTTCTTGGGAGCATAACGTCTTAGTGGCACACCACGACGCAATGTTAAGCCCTAATATCATTTATAACAACTCAAACCCATCGTTAATAGATATTATCTAATTTGACTCGTTACTATCTTTGTCAATTTCAAAATTTTTAAAATGCTTTTACTGG

Coding sequence (CDS)

ATGGCAGAAGCTTCACAGATTCTCTTACCGGAGTCGTTTCAAGGGGGAAGAGGCGATTTCACTGACCAAATTTGGCTCATATGGGAGCTCATCAAAGCGCCATTGATAGTTCCTGTGCTTAGATTAATGGTTTATATCTCATTGACAATGTCTCTCATGCTTTTCTTTGAAAGGCTTTATATGGGAATTGTCATCATTCTTGTTAAGCTGTTTTGGAAGAAGCCTGAGAAACGTTACAATTATGAACCCATTCAGGATGATTTGGAATTGGGAAGCTCCAATTTCCCTCATGTTCTCATTCAAATCCCTATGTTTAACGAAAGAGAGGTTTACAAGATCTCCATTGGAGCTGCTTGTGGACTTTCATGGCCGGCCGACCGCCTCGTCATCCAAGTTCTTGACGATTCAACCGACCCTGTCATTAAGCAAATGGTGGAACAAGAATGCCTAAGATGGGCAAGCAAAGGGATCAACATAACATACCAAATCAGAGAAACTAGAGGAGGATACAAAGCTGGAGCTCTGAAAGAAGGGCTTAAACGGAGCTACGTTAAACACTGTGAATATGTCGCCATTTTCGACGCCGATTTCAGGCCGGAGCCGGATTATCTCCGGCGGGCCATTCCGTTCTTAGTCCATAACTCAGATATTGCTCTGGTTCAAGCTCGCTGGAGATTCGTGAATGCAGATGAGTGCTTGTTGACCAGAATGCAAGAGATGTCTTTGGATTACCATTTCACAGTGGAGCAAGAAGTTGGGTCTGCTACCCATGCCTTCTTTGGCTTCAATGGAACTGCTGGTGTATGGAGAATTGCTGCAATTAATGAGGCAGGTGGGTGGAAGGACAGGACCACAGTGGAAGACATGGACCTTGCGGTTCGAGCTAGTCTTAGAGGCTGGAAATTTGTCTACTTAGGTGACCTGCAGGTAAAAAGTGAGCTTCCTAGTACTTTCAAAGCCTTCCGTTTTCAGCAGCACCGATGGTCTTGTGGCCCTGCCAATCTTTTCCGCAAAATGGTCATGGAAATTGTCAGAAACAAGAAAGTTAAATTCTGGAAGAAAGTTTATGTGATTTACAGTTTCTTCTTCGTTCGGAAGATCATTGCCCATATGGTTACTTTCTTCTTTTACTGTGTTGTTCTTCCCCTTACCATTTTGGTTCCTGAAGTGTATGTACCAATCTGGGGAGCTGTTTACATTCCTTCAATCATTACCATTTTGAACTCAGTGGGAACTCCAAGGTCAATTCACCTTCTGTTCTACTGGATTCTTTTCGAAAACGTGATGTCATTGCACCGCACGAAAGCAACTCTGATTGGTCTGTTCGAAGCGGGTCGAGCCAACGAATGGGTTGTGACTGAAAAACTTGGAGATGCTCTGAAGAACAAGGCAGCTGCTGATGCTGCCAAGCTGAAGGCAAATGCCAAAGTGCCTAAAATAAGGTTGAGGTGCAAGTTGGGAGACAGAATCAACACATTGGAGCTGGGATTTGCAGCGTTCCTGTTCTTGTGTGGTTGCTATGACTTTGTTCATGGGAAGAACTGCTACTTTGTGTACCTATTCCTTCAGACATTCAGCTTTCTGATCACTGGAATTGGCTATGTTGGCACCATCATCCCAAGCTCTTGA

Protein sequence

MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLYMGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACGLSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLKRSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEMSLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYSFFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLFYWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKIRLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIPSS
Homology
BLAST of CmaCh20G005480 vs. ExPASy Swiss-Prot
Match: Q9FNI7 (Glucomannan 4-beta-mannosyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=CSLA2 PE=1 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 5.3e-258
Identity = 441/536 (82.28%), Postives = 482/536 (89.93%), Query Frame = 0

Query: 6   QILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLYMGIVI 65
           + +LPE+F G R + T Q+ +IWEL+KAP+IVP+L+L VYI L MS+ML  ER+YMGIVI
Sbjct: 7   KFVLPETFDGVRMEITGQLGMIWELVKAPVIVPLLQLAVYICLLMSVMLLCERVYMGIVI 66

Query: 66  ILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACGLSWPA 125
           +LVKLFWKKP+KRY +EPI DD ELGSSNFP VL+QIPMFNEREVYK+SIGAACGLSWP+
Sbjct: 67  VLVKLFWKKPDKRYKFEPIHDDEELGSSNFPVVLVQIPMFNEREVYKLSIGAACGLSWPS 126

Query: 126 DRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLKRSYVK 185
           DRLVIQVLDDSTDP +KQMVE EC RWASKGINI YQIRE R GYKAGALKEGLKRSYVK
Sbjct: 127 DRLVIQVLDDSTDPTVKQMVEVECQRWASKGINIRYQIRENRVGYKAGALKEGLKRSYVK 186

Query: 186 HCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEMSLDYH 245
           HCEYV IFDADF+PEPD+LRR+IPFL+HN +IALVQARWRFVN+DECLLTRMQEMSLDYH
Sbjct: 187 HCEYVVIFDADFQPEPDFLRRSIPFLMHNPNIALVQARWRFVNSDECLLTRMQEMSLDYH 246

Query: 246 FTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKFVYL 305
           FTVEQEVGS+THAFFGFNGTAG+WRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKF+YL
Sbjct: 247 FTVEQEVGSSTHAFFGFNGTAGIWRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKFLYL 306

Query: 306 GDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYSFFFVR 365
           GDLQVKSELPSTF+AFRFQQHRWSCGPANLFRKMVMEIVRNKKV+FWKKVYVIYSFFFVR
Sbjct: 307 GDLQVKSELPSTFRAFRFQQHRWSCGPANLFRKMVMEIVRNKKVRFWKKVYVIYSFFFVR 366

Query: 366 KIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLFYWILF 425
           KIIAH VTF FYCVVLPLTILVPEV VPIWG+VYIPSIITILNSVGTPRSIHLLFYWILF
Sbjct: 367 KIIAHWVTFCFYCVVLPLTILVPEVKVPIWGSVYIPSIITILNSVGTPRSIHLLFYWILF 426

Query: 426 ENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKIRLRCK 485
           ENVMSLHRTKATLIGLFEAGRANEWVVT KLG     K      K     + P+I    K
Sbjct: 427 ENVMSLHRTKATLIGLFEAGRANEWVVTAKLGSGQSAKGNTKGIK-----RFPRI---FK 486

Query: 486 LGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIPS 542
           L DR+NTLELGFAAFLF+CGCYDFVHGKN YF+YLFLQT SF I+G+G++GT +PS
Sbjct: 487 LPDRLNTLELGFAAFLFVCGCYDFVHGKNNYFIYLFLQTMSFFISGLGWIGTYVPS 534

BLAST of CmaCh20G005480 vs. ExPASy Swiss-Prot
Match: Q9LZR3 (Glucomannan 4-beta-mannosyltransferase 9 OS=Arabidopsis thaliana OX=3702 GN=CSLA9 PE=1 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 2.0e-225
Identity = 376/534 (70.41%), Postives = 449/534 (84.08%), Query Frame = 0

Query: 8   LLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLYMGIVIIL 67
           ++P+SF G R D T Q+ ++ + I+APLIVP LRL VYI LTMS+MLF ER+YMGIVI L
Sbjct: 9   VIPDSFMGYRDDITMQMSMVLDQIRAPLIVPALRLGVYICLTMSVMLFVERVYMGIVISL 68

Query: 68  VKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACGLSWPADR 127
           VKLF +KP+KR+ YEPI+DD+ELG+S +P VLIQIPMFNEREVY++SIGAACGLSWP+DR
Sbjct: 69  VKLFGRKPDKRFKYEPIKDDIELGNSAYPMVLIQIPMFNEREVYQLSIGAACGLSWPSDR 128

Query: 128 LVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLKRSYVKHC 187
           +VIQVLDDSTDP IK +VE EC RWASKG+NI Y+IR+ R GYKAGALKEG+K+SYVK C
Sbjct: 129 IVIQVLDDSTDPTIKDLVEMECSRWASKGVNIKYEIRDNRNGYKAGALKEGMKKSYVKSC 188

Query: 188 EYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEMSLDYHFT 247
           +YVAIFDADF+PE D+L R +P+L+HN  +ALVQARW+FVN+DECL+TRMQEMSLDYHFT
Sbjct: 189 DYVAIFDADFQPEADFLWRTVPYLLHNPKLALVQARWKFVNSDECLMTRMQEMSLDYHFT 248

Query: 248 VEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKFVYLGD 307
           VEQEVGS+T+AFFGFNGTAG+WRI+A+NEAGGWKDRTTVEDMDLAVRASL+GWKF+YLG 
Sbjct: 249 VEQEVGSSTYAFFGFNGTAGIWRISALNEAGGWKDRTTVEDMDLAVRASLKGWKFLYLGS 308

Query: 308 LQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYSFFFVRKI 367
           L+VK+ELPSTFKA+R+QQHRWSCGPANLFRKM  EI+ NK V  WKKV+VIYSFF VRK+
Sbjct: 309 LKVKNELPSTFKAYRYQQHRWSCGPANLFRKMAFEIMTNKNVTLWKKVHVIYSFFVVRKL 368

Query: 368 IAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLFYWILFEN 427
           +AH+VTF FYCV+LP T+LVPEV VP WGAVYIPS+IT+LN+VGTPRS+HL+ +WILFEN
Sbjct: 369 VAHIVTFIFYCVILPATVLVPEVTVPKWGAVYIPSVITLLNAVGTPRSLHLMVFWILFEN 428

Query: 428 VMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKIRLRCKLG 487
           VMSLHRTKAT IGL E GR NEW+VTEKLGD           K K+  K  K  +R + G
Sbjct: 429 VMSLHRTKATFIGLLEGGRVNEWIVTEKLGD----------VKAKSATKTSKKVIRFRFG 488

Query: 488 DRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIPS 542
           DRI+ LELG   +L   GCYD   GKN Y++YLF Q  +F I G G +GTI+P+
Sbjct: 489 DRIHVLELGVGMYLLFVGCYDAFFGKNHYYLYLFAQAIAFFIAGFGQIGTIVPN 532

BLAST of CmaCh20G005480 vs. ExPASy Swiss-Prot
Match: Q7PC76 (Glucomannan 4-beta-mannosyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CSLA1 PE=1 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 3.2e-223
Identity = 382/516 (74.03%), Postives = 444/516 (86.05%), Query Frame = 0

Query: 28  WELIKAPLIVPVLRLMVYISLTMSLMLFFERLYMGIVIILVKLFWKKPEKRYNYEPI-QD 87
           W  ++AP+IVP+LRL V + LTMS++LF ER+YM +VI  VK+  ++P++RY  +PI  D
Sbjct: 15  WSQVRAPVIVPLLRLAVAVCLTMSVLLFLERMYMAVVISGVKILRRRPDRRYRCDPIPDD 74

Query: 88  DLELGSSNFPHVLIQIPMFNEREVYKISIGAACGLSWPADRLVIQVLDDSTDPVIKQMVE 147
           D ELG+S FP VLIQIPMFNEREVY++SIGA CGLSWP+DRLV+QVLDDSTDPVIK+MV 
Sbjct: 75  DPELGTSAFPVVLIQIPMFNEREVYQLSIGAVCGLSWPSDRLVVQVLDDSTDPVIKEMVR 134

Query: 148 QECLRWASKGINITYQIRETRGGYKAGALKEGLKRSYVKHCEYVAIFDADFRPEPDYLRR 207
            EC RWA KG+NITYQIRE R GYKAGALKEG+K  YV+ CEYVAIFDADF+P+PD+LRR
Sbjct: 135 IECERWAHKGVNITYQIRENRKGYKAGALKEGMKHGYVRECEYVAIFDADFQPDPDFLRR 194

Query: 208 AIPFLVHNSDIALVQARWRFVNADECLLTRMQEMSLDYHFTVEQEVGSATHAFFGFNGTA 267
            IPFLVHNSDIALVQARWRFVNADECL+TRMQEMSLDYHFTVEQEV S+  AFFGFNGTA
Sbjct: 195 TIPFLVHNSDIALVQARWRFVNADECLMTRMQEMSLDYHFTVEQEVSSSVCAFFGFNGTA 254

Query: 268 GVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKFVYLGDLQVKSELPSTFKAFRFQQH 327
           GVWR++A+NEAGGWKDRTTVEDMDLA+RASL+GWKFVYLGD+QVKSELPSTFKAFRFQQH
Sbjct: 255 GVWRVSAVNEAGGWKDRTTVEDMDLAIRASLKGWKFVYLGDVQVKSELPSTFKAFRFQQH 314

Query: 328 RWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYSFFFVRKIIAHMVTFFFYCVVLPLTIL 387
           RWSCGPANLFRKM+MEIVRNKKV  WKK++VIY+FF +RKIIAH+VTF FYC+++P TI 
Sbjct: 315 RWSCGPANLFRKMLMEIVRNKKVTIWKKIHVIYNFFLIRKIIAHIVTFAFYCLIIPATIF 374

Query: 388 VPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLFYWILFENVMSLHRTKATLIGLFEAGR 447
           VPEV +P WG VYIP+IIT+LNSVGTPRS HLLF+WILFENVMSLHRTKATLIGL EAGR
Sbjct: 375 VPEVRIPKWGCVYIPTIITLLNSVGTPRSFHLLFFWILFENVMSLHRTKATLIGLLEAGR 434

Query: 448 ANEWVVTEKLGDALKNKAAADAAKLKANAKVPKIRLRCKLGDRINTLELGFAAFLFLCGC 507
           ANEWVVTEKLG+ALK K+++     K++AK   +R    + DR+N  ELG AAFLF CG 
Sbjct: 435 ANEWVVTEKLGNALKMKSSS-----KSSAKKSFMR----VWDRLNVTELGVAAFLFSCGW 494

Query: 508 YDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIPSS 543
           YD   GK+ +F+YLF Q  +F I GIGYVGTI+P S
Sbjct: 495 YDLAFGKDHFFIYLFFQGAAFFIVGIGYVGTIVPQS 521

BLAST of CmaCh20G005480 vs. ExPASy Swiss-Prot
Match: Q67VS7 (Probable glucomannan 4-beta-mannosyltransferase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=CSLA9 PE=2 SV=1)

HSP 1 Score: 758.4 bits (1957), Expect = 5.4e-218
Identity = 366/527 (69.45%), Postives = 439/527 (83.30%), Query Frame = 0

Query: 22  DQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLYMGIVIILVKLFWKKPEKRYNY 81
           +QI  +WE +KAP++VP+LRL V   L MS+MLF E++YM +V++ V LF ++P++RY  
Sbjct: 10  EQIAAMWEQVKAPVVVPLLRLSVAACLAMSVMLFVEKVYMSVVLVGVHLFGRRPDRRYRC 69

Query: 82  EPI------QDDLELGSSN--FPHVLIQIPMFNEREVYKISIGAACGLSWPADRLVIQVL 141
           +PI       DD EL  +N  FP VLIQIPM+NEREVYK+SIGAACGLSWP+DR+++QVL
Sbjct: 70  DPIVAAGADNDDPELADANAAFPMVLIQIPMYNEREVYKLSIGAACGLSWPSDRVIVQVL 129

Query: 142 DDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLKRSYVKHCEYVAIF 201
           DDSTDPVIK+MV+ EC RW SKG+ I Y+IR+ R GYKAGAL+EG+K  YV+ C+YVAIF
Sbjct: 130 DDSTDPVIKEMVQVECKRWESKGVRIKYEIRDNRVGYKAGALREGMKHGYVRDCDYVAIF 189

Query: 202 DADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEMSLDYHFTVEQEVG 261
           DADF+P+PD+L R IPFLVHN DIALVQARW+FVNA+ECL+TRMQEMSLDYHF VEQEVG
Sbjct: 190 DADFQPDPDFLARTIPFLVHNPDIALVQARWKFVNANECLMTRMQEMSLDYHFKVEQEVG 249

Query: 262 SATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKFVYLGDLQVKSE 321
           S+THAFFGFNGTAGVWRI+A+NEAGGWKDRTTVEDMDLAVRA L+GWKFVYLGDL VKSE
Sbjct: 250 SSTHAFFGFNGTAGVWRISAMNEAGGWKDRTTVEDMDLAVRAGLKGWKFVYLGDLMVKSE 309

Query: 322 LPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYSFFFVRKIIAHMVT 381
           LPSTFKAFR+QQHRWSCGPANLFRKM++EI  NKKV  WKK+YVIY+FF VRKII H+VT
Sbjct: 310 LPSTFKAFRYQQHRWSCGPANLFRKMLVEIATNKKVTLWKKIYVIYNFFLVRKIIGHIVT 369

Query: 382 FFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLFYWILFENVMSLHR 441
           F FYC+V+P T+L+PEV +P WG VY+PSI+TILNS+GTPRS+HLL +W+LFENVMSLHR
Sbjct: 370 FVFYCLVVPATVLIPEVEIPRWGYVYLPSIVTILNSIGTPRSLHLLIFWVLFENVMSLHR 429

Query: 442 TKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKIRLRCKLGDRINTL 501
           TKATLIGL E GR NEWVVTEKLGDALK K    A +          R R ++GDR+N L
Sbjct: 430 TKATLIGLLETGRVNEWVVTEKLGDALKLKLPGKAFR----------RPRMRIGDRVNAL 489

Query: 502 ELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 541
           ELGF+A+L  CGCYD  +GK  Y ++LFLQ+ +F I G+GYVGTI+P
Sbjct: 490 ELGFSAYLSFCGCYDIAYGKGYYSLFLFLQSITFFIIGVGYVGTIVP 526

BLAST of CmaCh20G005480 vs. ExPASy Swiss-Prot
Match: Q9LQC9 (Probable glucomannan 4-beta-mannosyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=CSLA3 PE=2 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 4.3e-199
Identity = 330/526 (62.74%), Postives = 416/526 (79.09%), Query Frame = 0

Query: 17  RGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLYMGIVIILVKLFWKKPE 76
           R     +I  IW+  +  + +P+L+ +V I L MSL+LF ER+YM IV++ VKL  + PE
Sbjct: 40  RSGIIGEIIYIWKQTRIFVFIPILKCLVTICLVMSLLLFIERVYMSIVVVFVKLLRRTPE 99

Query: 77  KRYNYEPI-QDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACGLSWPADRLVIQVLDD 136
           K + +EPI  DDLEL ++N+P VLIQIPM+NE+EV ++SIGAAC LSWP DR+++QVLDD
Sbjct: 100 KVHKWEPINDDDLELANTNYPMVLIQIPMYNEKEVCQLSIGAACRLSWPLDRMIVQVLDD 159

Query: 137 STDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLKRSYVKHCEYVAIFDA 196
           STDP  K++V  EC +WA KGINI  +IR+ R GYKAGALK G+  +YVK CE+VAIFDA
Sbjct: 160 STDPASKELVNAECDKWARKGINIMSEIRDNRIGYKAGALKAGMMHNYVKQCEFVAIFDA 219

Query: 197 DFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEMSLDYHFTVEQEVGSA 256
           DF+P+PD+L R IPFL+HN +I+LVQ RW+FVNA+ECL+TRMQEMSL+YHF  EQE GS+
Sbjct: 220 DFQPDPDFLERTIPFLIHNHEISLVQCRWKFVNANECLMTRMQEMSLNYHFVAEQESGSS 279

Query: 257 THAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKFVYLGDLQVKSELP 316
            HAFFGFNGTAGVWRIAA+NEAGGWKDRTTVEDMDLAVRA L GWKFVY+ D++VK+ELP
Sbjct: 280 IHAFFGFNGTAGVWRIAALNEAGGWKDRTTVEDMDLAVRACLHGWKFVYVHDVEVKNELP 339

Query: 317 STFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYSFFFVRKIIAHMVTFF 376
           STFKA+RFQQHRWSCGPANL+RKM MEI++NKKV  WKK+Y+IY+FFF+RKI+ H+ TF 
Sbjct: 340 STFKAYRFQQHRWSCGPANLWRKMTMEILQNKKVSAWKKLYLIYNFFFIRKIVVHIFTFV 399

Query: 377 FYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLFYWILFENVMSLHRTK 436
           FYC++LP T+L PE+ VP W  VY P+ ITILN++ TPRS+HLL +WILFENVMS+HRTK
Sbjct: 400 FYCLILPTTVLFPELQVPKWATVYFPTTITILNAIATPRSLHLLVFWILFENVMSMHRTK 459

Query: 437 ATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKIRLRCKLGDRINTLEL 496
           AT IGL EAGR NEWVVTEKLGD LK+K    A            +L  + G R+N  EL
Sbjct: 460 ATFIGLLEAGRVNEWVVTEKLGDTLKSKLIGKAT----------TKLYTRFGQRLNWREL 519

Query: 497 GFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIPS 542
               ++F CGCYDF +G + ++VYLFLQ+ +F + G+GY+GT +P+
Sbjct: 520 VVGLYIFFCGCYDFAYGGSYFYVYLFLQSCAFFVAGVGYIGTFVPT 555

BLAST of CmaCh20G005480 vs. ExPASy TrEMBL
Match: A0A6J1JDM3 (glucomannan 4-beta-mannosyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111484076 PE=4 SV=1)

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 542/542 (100.00%), Postives = 542/542 (100.00%), Query Frame = 0

Query: 1   MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY 60
           MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY
Sbjct: 1   MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY 60

Query: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120
           MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG
Sbjct: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120

Query: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180
           LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK
Sbjct: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180

Query: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM 240
           RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM
Sbjct: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM 240

Query: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300
           SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW
Sbjct: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300

Query: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360
           KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS
Sbjct: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360

Query: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420
           FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF
Sbjct: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420

Query: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480
           YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI
Sbjct: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480

Query: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540
           RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP
Sbjct: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 542

BLAST of CmaCh20G005480 vs. ExPASy TrEMBL
Match: A0A6J1FXT3 (glucomannan 4-beta-mannosyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111448714 PE=4 SV=1)

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 539/542 (99.45%), Postives = 540/542 (99.63%), Query Frame = 0

Query: 1   MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY 60
           MAEASQILLPESFQGGRGDF+DQIWLIWELIKAPLIVPVLRLMVYISL MSLMLFFERLY
Sbjct: 1   MAEASQILLPESFQGGRGDFSDQIWLIWELIKAPLIVPVLRLMVYISLAMSLMLFFERLY 60

Query: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120
           MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG
Sbjct: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120

Query: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180
           LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK
Sbjct: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180

Query: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM 240
           RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHN DIALVQARWRFVNADECLLTRMQEM
Sbjct: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNPDIALVQARWRFVNADECLLTRMQEM 240

Query: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300
           SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW
Sbjct: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300

Query: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360
           KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS
Sbjct: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360

Query: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420
           FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF
Sbjct: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420

Query: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480
           YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI
Sbjct: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480

Query: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540
           RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP
Sbjct: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 542

BLAST of CmaCh20G005480 vs. ExPASy TrEMBL
Match: A0A1S3C7U5 (glucomannan 4-beta-mannosyltransferase 2 OS=Cucumis melo OX=3656 GN=LOC103497977 PE=4 SV=1)

HSP 1 Score: 1049.7 bits (2713), Expect = 4.3e-303
Identity = 525/542 (96.86%), Postives = 529/542 (97.60%), Query Frame = 0

Query: 1   MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY 60
           MAE +QILLPESFQGGRGDFT+QIWLIWELIKAPLIVPVLRLMVYISL MSLMLFFERLY
Sbjct: 1   MAETTQILLPESFQGGRGDFTEQIWLIWELIKAPLIVPVLRLMVYISLAMSLMLFFERLY 60

Query: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120
           MGIVIILVKLFWKKPEKRY YEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG
Sbjct: 61  MGIVIILVKLFWKKPEKRYKYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120

Query: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180
           LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK
Sbjct: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180

Query: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM 240
           RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHN DIALVQARWRFVNADECLLTRMQEM
Sbjct: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNPDIALVQARWRFVNADECLLTRMQEM 240

Query: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300
           SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW
Sbjct: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300

Query: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360
           KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS
Sbjct: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360

Query: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420
           FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF
Sbjct: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420

Query: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480
           YWILFENVMSLHRTKATLIGL EAGRANEWVVTEKLGDALKNKAAAD    KA  K+PKI
Sbjct: 421 YWILFENVMSLHRTKATLIGLLEAGRANEWVVTEKLGDALKNKAAADK---KAGGKIPKI 480

Query: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540
           RLRCK GDRINTLELGFAAFLFLCGCYDFVHGKN YF+YLFLQTFSFLITGIGYVGTIIP
Sbjct: 481 RLRCKFGDRINTLELGFAAFLFLCGCYDFVHGKNNYFIYLFLQTFSFLITGIGYVGTIIP 539

Query: 541 SS 543
           SS
Sbjct: 541 SS 539

BLAST of CmaCh20G005480 vs. ExPASy TrEMBL
Match: A0A6J1JZW1 (glucomannan 4-beta-mannosyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111491203 PE=4 SV=1)

HSP 1 Score: 1043.5 bits (2697), Expect = 3.1e-301
Identity = 518/542 (95.57%), Postives = 529/542 (97.60%), Query Frame = 0

Query: 1   MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY 60
           MA+A+QILLPESFQGGRG+F+DQIWLIWELIKAPLIVP+LRLMVYISL MSLMLFFER+Y
Sbjct: 1   MADATQILLPESFQGGRGNFSDQIWLIWELIKAPLIVPLLRLMVYISLAMSLMLFFERVY 60

Query: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120
           MGIVI+LVKLFWKKPEKRY YE +QDDLE GSSNFPHVLIQIPMFNEREVYKISIGAACG
Sbjct: 61  MGIVIVLVKLFWKKPEKRYKYELLQDDLESGSSNFPHVLIQIPMFNEREVYKISIGAACG 120

Query: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180
           LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK
Sbjct: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180

Query: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM 240
           R YV HCEYVAIFDADFRPEPDYLRRAIPFLVHN DIALVQARWRFVNA+ECLLTRMQEM
Sbjct: 181 RRYVSHCEYVAIFDADFRPEPDYLRRAIPFLVHNPDIALVQARWRFVNANECLLTRMQEM 240

Query: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300
           SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW
Sbjct: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300

Query: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360
           KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS
Sbjct: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360

Query: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420
           FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPS+ITILNSVGTPRSIHLLF
Sbjct: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSVITILNSVGTPRSIHLLF 420

Query: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480
           YWILFENVMSLHRTKA LIGLFEAGR NEWVVTEKLGDALKNKAAADAAK KANAKVPKI
Sbjct: 421 YWILFENVMSLHRTKAALIGLFEAGRVNEWVVTEKLGDALKNKAAADAAKSKANAKVPKI 480

Query: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540
           RLRCK GDRINTLELGFAAFLF CGCYDFVHGKN YFVYLFLQTFSFLITG+GYVGTIIP
Sbjct: 481 RLRCKFGDRINTLELGFAAFLFFCGCYDFVHGKNNYFVYLFLQTFSFLITGVGYVGTIIP 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 542

BLAST of CmaCh20G005480 vs. ExPASy TrEMBL
Match: A0A6J1H325 (glucomannan 4-beta-mannosyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111459651 PE=4 SV=1)

HSP 1 Score: 1043.5 bits (2697), Expect = 3.1e-301
Identity = 518/542 (95.57%), Postives = 529/542 (97.60%), Query Frame = 0

Query: 1   MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY 60
           MA+A+QILLPESFQGGRG+F+DQIWLIWELIKAPLIVP+LRLMVYISL MSLMLFFER+Y
Sbjct: 1   MADATQILLPESFQGGRGNFSDQIWLIWELIKAPLIVPLLRLMVYISLAMSLMLFFERVY 60

Query: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120
           MGIVI+LVKLFWKKPEKRY YE +QDDLE GSSNFPHVLIQIPMFNEREVYKISIGAACG
Sbjct: 61  MGIVIVLVKLFWKKPEKRYKYELLQDDLESGSSNFPHVLIQIPMFNEREVYKISIGAACG 120

Query: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180
           LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK
Sbjct: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180

Query: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM 240
           R YV HCEYVAIFDADFRPEPDYLRRAIPFLVHN DIALVQARWRFVNA+ECLLTRMQEM
Sbjct: 181 RRYVSHCEYVAIFDADFRPEPDYLRRAIPFLVHNPDIALVQARWRFVNANECLLTRMQEM 240

Query: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300
           SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW
Sbjct: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300

Query: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360
           KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS
Sbjct: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360

Query: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420
           FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPS+ITILNSVGTPRSIHLLF
Sbjct: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSVITILNSVGTPRSIHLLF 420

Query: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480
           YWILFENVMSLHRTKA LIGLFEAGR NEWVVTEKLGDALKNKAAADAAK KANAKVPKI
Sbjct: 421 YWILFENVMSLHRTKAALIGLFEAGRVNEWVVTEKLGDALKNKAAADAAKSKANAKVPKI 480

Query: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540
           RLRCK GDRINTLELGFAAFLF CGCYDFVHGKN YFVYLFLQTFSFLITG+GYVGTIIP
Sbjct: 481 RLRCKFGDRINTLELGFAAFLFFCGCYDFVHGKNNYFVYLFLQTFSFLITGVGYVGTIIP 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 542

BLAST of CmaCh20G005480 vs. NCBI nr
Match: XP_022986280.1 (glucomannan 4-beta-mannosyltransferase 2-like [Cucurbita maxima])

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 542/542 (100.00%), Postives = 542/542 (100.00%), Query Frame = 0

Query: 1   MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY 60
           MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY
Sbjct: 1   MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY 60

Query: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120
           MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG
Sbjct: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120

Query: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180
           LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK
Sbjct: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180

Query: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM 240
           RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM
Sbjct: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM 240

Query: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300
           SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW
Sbjct: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300

Query: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360
           KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS
Sbjct: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360

Query: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420
           FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF
Sbjct: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420

Query: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480
           YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI
Sbjct: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480

Query: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540
           RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP
Sbjct: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 542

BLAST of CmaCh20G005480 vs. NCBI nr
Match: XP_022944193.1 (glucomannan 4-beta-mannosyltransferase 2-like [Cucurbita moschata] >KAG6570842.1 Glucomannan 4-beta-mannosyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010688.1 Glucomannan 4-beta-mannosyltransferase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 539/542 (99.45%), Postives = 540/542 (99.63%), Query Frame = 0

Query: 1   MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY 60
           MAEASQILLPESFQGGRGDF+DQIWLIWELIKAPLIVPVLRLMVYISL MSLMLFFERLY
Sbjct: 1   MAEASQILLPESFQGGRGDFSDQIWLIWELIKAPLIVPVLRLMVYISLAMSLMLFFERLY 60

Query: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120
           MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG
Sbjct: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120

Query: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180
           LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK
Sbjct: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180

Query: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM 240
           RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHN DIALVQARWRFVNADECLLTRMQEM
Sbjct: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNPDIALVQARWRFVNADECLLTRMQEM 240

Query: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300
           SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW
Sbjct: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300

Query: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360
           KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS
Sbjct: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360

Query: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420
           FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF
Sbjct: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420

Query: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480
           YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI
Sbjct: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480

Query: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540
           RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP
Sbjct: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 542

BLAST of CmaCh20G005480 vs. NCBI nr
Match: XP_023522032.1 (glucomannan 4-beta-mannosyltransferase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1059.3 bits (2738), Expect = 1.1e-305
Identity = 527/542 (97.23%), Postives = 532/542 (98.15%), Query Frame = 0

Query: 1   MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY 60
           MAEASQ+LLPESFQGGRGDFTDQIWLIW+LIKAPLIVPVLRLMVYISL MSLMLFFERLY
Sbjct: 1   MAEASQVLLPESFQGGRGDFTDQIWLIWDLIKAPLIVPVLRLMVYISLAMSLMLFFERLY 60

Query: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120
           MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG
Sbjct: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120

Query: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180
           LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK
Sbjct: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180

Query: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM 240
           R YV HCEYVAIFDADFRPEPDYLRRAIPFLVHN DIALVQARWRFVNA+ECLLTRMQEM
Sbjct: 181 RRYVSHCEYVAIFDADFRPEPDYLRRAIPFLVHNPDIALVQARWRFVNANECLLTRMQEM 240

Query: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300
           SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW
Sbjct: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300

Query: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360
           KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS
Sbjct: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360

Query: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420
           FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPS+ITILNSVGTPRSIHLLF
Sbjct: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSVITILNSVGTPRSIHLLF 420

Query: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480
           YWILFENVMSLHRTKA LIGLFEAGR NEWVVTEKLGDALKNKAAADAAK KANAKVPKI
Sbjct: 421 YWILFENVMSLHRTKAALIGLFEAGRVNEWVVTEKLGDALKNKAAADAAKNKANAKVPKI 480

Query: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540
           RLRCK GDRINTLELGFAAFLF CGCYDFVHGKN YFVYLFLQTFSFLITG+GYVGTIIP
Sbjct: 481 RLRCKFGDRINTLELGFAAFLFFCGCYDFVHGKNNYFVYLFLQTFSFLITGVGYVGTIIP 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 542

BLAST of CmaCh20G005480 vs. NCBI nr
Match: XP_008458630.1 (PREDICTED: glucomannan 4-beta-mannosyltransferase 2 [Cucumis melo])

HSP 1 Score: 1049.7 bits (2713), Expect = 8.9e-303
Identity = 525/542 (96.86%), Postives = 529/542 (97.60%), Query Frame = 0

Query: 1   MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY 60
           MAE +QILLPESFQGGRGDFT+QIWLIWELIKAPLIVPVLRLMVYISL MSLMLFFERLY
Sbjct: 1   MAETTQILLPESFQGGRGDFTEQIWLIWELIKAPLIVPVLRLMVYISLAMSLMLFFERLY 60

Query: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120
           MGIVIILVKLFWKKPEKRY YEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG
Sbjct: 61  MGIVIILVKLFWKKPEKRYKYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120

Query: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180
           LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK
Sbjct: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180

Query: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM 240
           RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHN DIALVQARWRFVNADECLLTRMQEM
Sbjct: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNPDIALVQARWRFVNADECLLTRMQEM 240

Query: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300
           SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW
Sbjct: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300

Query: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360
           KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS
Sbjct: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360

Query: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420
           FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF
Sbjct: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420

Query: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480
           YWILFENVMSLHRTKATLIGL EAGRANEWVVTEKLGDALKNKAAAD    KA  K+PKI
Sbjct: 421 YWILFENVMSLHRTKATLIGLLEAGRANEWVVTEKLGDALKNKAAADK---KAGGKIPKI 480

Query: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540
           RLRCK GDRINTLELGFAAFLFLCGCYDFVHGKN YF+YLFLQTFSFLITGIGYVGTIIP
Sbjct: 481 RLRCKFGDRINTLELGFAAFLFLCGCYDFVHGKNNYFIYLFLQTFSFLITGIGYVGTIIP 539

Query: 541 SS 543
           SS
Sbjct: 541 SS 539

BLAST of CmaCh20G005480 vs. NCBI nr
Match: XP_038901424.1 (glucomannan 4-beta-mannosyltransferase 2 [Benincasa hispida] >XP_038901425.1 glucomannan 4-beta-mannosyltransferase 2 [Benincasa hispida] >XP_038901427.1 glucomannan 4-beta-mannosyltransferase 2 [Benincasa hispida])

HSP 1 Score: 1047.7 bits (2708), Expect = 3.4e-302
Identity = 522/542 (96.31%), Postives = 529/542 (97.60%), Query Frame = 0

Query: 1   MAEASQILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLY 60
           MAE +QILLPESFQGGRGDFT+QIWLIWELIKAPLIVP+LRLMVYISL MSLMLFFERLY
Sbjct: 1   MAETTQILLPESFQGGRGDFTEQIWLIWELIKAPLIVPILRLMVYISLAMSLMLFFERLY 60

Query: 61  MGIVIILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACG 120
           MG+VIILVKLFWKKPEKRY YEPIQDDLELGS+NFPHVLIQIPMFNEREVYK+SIGAACG
Sbjct: 61  MGVVIILVKLFWKKPEKRYKYEPIQDDLELGSTNFPHVLIQIPMFNEREVYKVSIGAACG 120

Query: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180
           LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK
Sbjct: 121 LSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLK 180

Query: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEM 240
           RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHN DIALVQARWRFVNADECLLTRMQEM
Sbjct: 181 RSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNPDIALVQARWRFVNADECLLTRMQEM 240

Query: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300
           SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW
Sbjct: 241 SLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGW 300

Query: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360
           KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS
Sbjct: 301 KFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYS 360

Query: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420
           FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF
Sbjct: 361 FFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLF 420

Query: 421 YWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKI 480
           YWILFENVMSLHRTKATLIGL EAGRANEWVVTEKLGD LKNKAAAD  K KA  K+PKI
Sbjct: 421 YWILFENVMSLHRTKATLIGLLEAGRANEWVVTEKLGDTLKNKAAAD--KNKAGGKIPKI 480

Query: 481 RLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIP 540
           RLRCK GDRINTLELGFAAFLFLCGCYDFVHGKN YFVYLFLQTFSFLITGIGYVGTIIP
Sbjct: 481 RLRCKFGDRINTLELGFAAFLFLCGCYDFVHGKNNYFVYLFLQTFSFLITGIGYVGTIIP 540

Query: 541 SS 543
           SS
Sbjct: 541 SS 540

BLAST of CmaCh20G005480 vs. TAIR 10
Match: AT5G22740.1 (cellulose synthase-like A02 )

HSP 1 Score: 891.3 bits (2302), Expect = 3.8e-259
Identity = 441/536 (82.28%), Postives = 482/536 (89.93%), Query Frame = 0

Query: 6   QILLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLYMGIVI 65
           + +LPE+F G R + T Q+ +IWEL+KAP+IVP+L+L VYI L MS+ML  ER+YMGIVI
Sbjct: 7   KFVLPETFDGVRMEITGQLGMIWELVKAPVIVPLLQLAVYICLLMSVMLLCERVYMGIVI 66

Query: 66  ILVKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACGLSWPA 125
           +LVKLFWKKP+KRY +EPI DD ELGSSNFP VL+QIPMFNEREVYK+SIGAACGLSWP+
Sbjct: 67  VLVKLFWKKPDKRYKFEPIHDDEELGSSNFPVVLVQIPMFNEREVYKLSIGAACGLSWPS 126

Query: 126 DRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLKRSYVK 185
           DRLVIQVLDDSTDP +KQMVE EC RWASKGINI YQIRE R GYKAGALKEGLKRSYVK
Sbjct: 127 DRLVIQVLDDSTDPTVKQMVEVECQRWASKGINIRYQIRENRVGYKAGALKEGLKRSYVK 186

Query: 186 HCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEMSLDYH 245
           HCEYV IFDADF+PEPD+LRR+IPFL+HN +IALVQARWRFVN+DECLLTRMQEMSLDYH
Sbjct: 187 HCEYVVIFDADFQPEPDFLRRSIPFLMHNPNIALVQARWRFVNSDECLLTRMQEMSLDYH 246

Query: 246 FTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKFVYL 305
           FTVEQEVGS+THAFFGFNGTAG+WRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKF+YL
Sbjct: 247 FTVEQEVGSSTHAFFGFNGTAGIWRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKFLYL 306

Query: 306 GDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYSFFFVR 365
           GDLQVKSELPSTF+AFRFQQHRWSCGPANLFRKMVMEIVRNKKV+FWKKVYVIYSFFFVR
Sbjct: 307 GDLQVKSELPSTFRAFRFQQHRWSCGPANLFRKMVMEIVRNKKVRFWKKVYVIYSFFFVR 366

Query: 366 KIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLFYWILF 425
           KIIAH VTF FYCVVLPLTILVPEV VPIWG+VYIPSIITILNSVGTPRSIHLLFYWILF
Sbjct: 367 KIIAHWVTFCFYCVVLPLTILVPEVKVPIWGSVYIPSIITILNSVGTPRSIHLLFYWILF 426

Query: 426 ENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKIRLRCK 485
           ENVMSLHRTKATLIGLFEAGRANEWVVT KLG     K      K     + P+I    K
Sbjct: 427 ENVMSLHRTKATLIGLFEAGRANEWVVTAKLGSGQSAKGNTKGIK-----RFPRI---FK 486

Query: 486 LGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIPS 542
           L DR+NTLELGFAAFLF+CGCYDFVHGKN YF+YLFLQT SF I+G+G++GT +PS
Sbjct: 487 LPDRLNTLELGFAAFLFVCGCYDFVHGKNNYFIYLFLQTMSFFISGLGWIGTYVPS 534

BLAST of CmaCh20G005480 vs. TAIR 10
Match: AT5G03760.1 (Nucleotide-diphospho-sugar transferases superfamily protein )

HSP 1 Score: 783.1 bits (2021), Expect = 1.4e-226
Identity = 376/534 (70.41%), Postives = 449/534 (84.08%), Query Frame = 0

Query: 8   LLPESFQGGRGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLYMGIVIIL 67
           ++P+SF G R D T Q+ ++ + I+APLIVP LRL VYI LTMS+MLF ER+YMGIVI L
Sbjct: 9   VIPDSFMGYRDDITMQMSMVLDQIRAPLIVPALRLGVYICLTMSVMLFVERVYMGIVISL 68

Query: 68  VKLFWKKPEKRYNYEPIQDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACGLSWPADR 127
           VKLF +KP+KR+ YEPI+DD+ELG+S +P VLIQIPMFNEREVY++SIGAACGLSWP+DR
Sbjct: 69  VKLFGRKPDKRFKYEPIKDDIELGNSAYPMVLIQIPMFNEREVYQLSIGAACGLSWPSDR 128

Query: 128 LVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLKRSYVKHC 187
           +VIQVLDDSTDP IK +VE EC RWASKG+NI Y+IR+ R GYKAGALKEG+K+SYVK C
Sbjct: 129 IVIQVLDDSTDPTIKDLVEMECSRWASKGVNIKYEIRDNRNGYKAGALKEGMKKSYVKSC 188

Query: 188 EYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEMSLDYHFT 247
           +YVAIFDADF+PE D+L R +P+L+HN  +ALVQARW+FVN+DECL+TRMQEMSLDYHFT
Sbjct: 189 DYVAIFDADFQPEADFLWRTVPYLLHNPKLALVQARWKFVNSDECLMTRMQEMSLDYHFT 248

Query: 248 VEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKFVYLGD 307
           VEQEVGS+T+AFFGFNGTAG+WRI+A+NEAGGWKDRTTVEDMDLAVRASL+GWKF+YLG 
Sbjct: 249 VEQEVGSSTYAFFGFNGTAGIWRISALNEAGGWKDRTTVEDMDLAVRASLKGWKFLYLGS 308

Query: 308 LQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYSFFFVRKI 367
           L+VK+ELPSTFKA+R+QQHRWSCGPANLFRKM  EI+ NK V  WKKV+VIYSFF VRK+
Sbjct: 309 LKVKNELPSTFKAYRYQQHRWSCGPANLFRKMAFEIMTNKNVTLWKKVHVIYSFFVVRKL 368

Query: 368 IAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLFYWILFEN 427
           +AH+VTF FYCV+LP T+LVPEV VP WGAVYIPS+IT+LN+VGTPRS+HL+ +WILFEN
Sbjct: 369 VAHIVTFIFYCVILPATVLVPEVTVPKWGAVYIPSVITLLNAVGTPRSLHLMVFWILFEN 428

Query: 428 VMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKIRLRCKLG 487
           VMSLHRTKAT IGL E GR NEW+VTEKLGD           K K+  K  K  +R + G
Sbjct: 429 VMSLHRTKATFIGLLEGGRVNEWIVTEKLGD----------VKAKSATKTSKKVIRFRFG 488

Query: 488 DRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIPS 542
           DRI+ LELG   +L   GCYD   GKN Y++YLF Q  +F I G G +GTI+P+
Sbjct: 489 DRIHVLELGVGMYLLFVGCYDAFFGKNHYYLYLFAQAIAFFIAGFGQIGTIVPN 532

BLAST of CmaCh20G005480 vs. TAIR 10
Match: AT1G23480.1 (cellulose synthase-like A3 )

HSP 1 Score: 695.7 bits (1794), Expect = 3.0e-200
Identity = 330/526 (62.74%), Postives = 416/526 (79.09%), Query Frame = 0

Query: 17  RGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLYMGIVIILVKLFWKKPE 76
           R     +I  IW+  +  + +P+L+ +V I L MSL+LF ER+YM IV++ VKL  + PE
Sbjct: 40  RSGIIGEIIYIWKQTRIFVFIPILKCLVTICLVMSLLLFIERVYMSIVVVFVKLLRRTPE 99

Query: 77  KRYNYEPI-QDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACGLSWPADRLVIQVLDD 136
           K + +EPI  DDLEL ++N+P VLIQIPM+NE+EV ++SIGAAC LSWP DR+++QVLDD
Sbjct: 100 KVHKWEPINDDDLELANTNYPMVLIQIPMYNEKEVCQLSIGAACRLSWPLDRMIVQVLDD 159

Query: 137 STDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLKRSYVKHCEYVAIFDA 196
           STDP  K++V  EC +WA KGINI  +IR+ R GYKAGALK G+  +YVK CE+VAIFDA
Sbjct: 160 STDPASKELVNAECDKWARKGINIMSEIRDNRIGYKAGALKAGMMHNYVKQCEFVAIFDA 219

Query: 197 DFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEMSLDYHFTVEQEVGSA 256
           DF+P+PD+L R IPFL+HN +I+LVQ RW+FVNA+ECL+TRMQEMSL+YHF  EQE GS+
Sbjct: 220 DFQPDPDFLERTIPFLIHNHEISLVQCRWKFVNANECLMTRMQEMSLNYHFVAEQESGSS 279

Query: 257 THAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKFVYLGDLQVKSELP 316
            HAFFGFNGTAGVWRIAA+NEAGGWKDRTTVEDMDLAVRA L GWKFVY+ D++VK+ELP
Sbjct: 280 IHAFFGFNGTAGVWRIAALNEAGGWKDRTTVEDMDLAVRACLHGWKFVYVHDVEVKNELP 339

Query: 317 STFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYSFFFVRKIIAHMVTFF 376
           STFKA+RFQQHRWSCGPANL+RKM MEI++NKKV  WKK+Y+IY+FFF+RKI+ H+ TF 
Sbjct: 340 STFKAYRFQQHRWSCGPANLWRKMTMEILQNKKVSAWKKLYLIYNFFFIRKIVVHIFTFV 399

Query: 377 FYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLFYWILFENVMSLHRTK 436
           FYC++LP T+L PE+ VP W  VY P+ ITILN++ TPRS+HLL +WILFENVMS+HRTK
Sbjct: 400 FYCLILPTTVLFPELQVPKWATVYFPTTITILNAIATPRSLHLLVFWILFENVMSMHRTK 459

Query: 437 ATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKIRLRCKLGDRINTLEL 496
           AT IGL EAGR NEWVVTEKLGD LK+K    A            +L  + G R+N  EL
Sbjct: 460 ATFIGLLEAGRVNEWVVTEKLGDTLKSKLIGKAT----------TKLYTRFGQRLNWREL 519

Query: 497 GFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIPS 542
               ++F CGCYDF +G + ++VYLFLQ+ +F + G+GY+GT +P+
Sbjct: 520 VVGLYIFFCGCYDFAYGGSYFYVYLFLQSCAFFVAGVGYIGTFVPT 555

BLAST of CmaCh20G005480 vs. TAIR 10
Match: AT1G23480.2 (cellulose synthase-like A3 )

HSP 1 Score: 695.7 bits (1794), Expect = 3.0e-200
Identity = 330/526 (62.74%), Postives = 416/526 (79.09%), Query Frame = 0

Query: 17  RGDFTDQIWLIWELIKAPLIVPVLRLMVYISLTMSLMLFFERLYMGIVIILVKLFWKKPE 76
           R     +I  IW+  +  + +P+L+ +V I L MSL+LF ER+YM IV++ VKL  + PE
Sbjct: 40  RSGIIGEIIYIWKQTRIFVFIPILKCLVTICLVMSLLLFIERVYMSIVVVFVKLLRRTPE 99

Query: 77  KRYNYEPI-QDDLELGSSNFPHVLIQIPMFNEREVYKISIGAACGLSWPADRLVIQVLDD 136
           K + +EPI  DDLEL ++N+P VLIQIPM+NE+EV ++SIGAAC LSWP DR+++QVLDD
Sbjct: 100 KVHKWEPINDDDLELANTNYPMVLIQIPMYNEKEVCQLSIGAACRLSWPLDRMIVQVLDD 159

Query: 137 STDPVIKQMVEQECLRWASKGINITYQIRETRGGYKAGALKEGLKRSYVKHCEYVAIFDA 196
           STDP  K++V  EC +WA KGINI  +IR+ R GYKAGALK G+  +YVK CE+VAIFDA
Sbjct: 160 STDPASKELVNAECDKWARKGINIMSEIRDNRIGYKAGALKAGMMHNYVKQCEFVAIFDA 219

Query: 197 DFRPEPDYLRRAIPFLVHNSDIALVQARWRFVNADECLLTRMQEMSLDYHFTVEQEVGSA 256
           DF+P+PD+L R IPFL+HN +I+LVQ RW+FVNA+ECL+TRMQEMSL+YHF  EQE GS+
Sbjct: 220 DFQPDPDFLERTIPFLIHNHEISLVQCRWKFVNANECLMTRMQEMSLNYHFVAEQESGSS 279

Query: 257 THAFFGFNGTAGVWRIAAINEAGGWKDRTTVEDMDLAVRASLRGWKFVYLGDLQVKSELP 316
            HAFFGFNGTAGVWRIAA+NEAGGWKDRTTVEDMDLAVRA L GWKFVY+ D++VK+ELP
Sbjct: 280 IHAFFGFNGTAGVWRIAALNEAGGWKDRTTVEDMDLAVRACLHGWKFVYVHDVEVKNELP 339

Query: 317 STFKAFRFQQHRWSCGPANLFRKMVMEIVRNKKVKFWKKVYVIYSFFFVRKIIAHMVTFF 376
           STFKA+RFQQHRWSCGPANL+RKM MEI++NKKV  WKK+Y+IY+FFF+RKI+ H+ TF 
Sbjct: 340 STFKAYRFQQHRWSCGPANLWRKMTMEILQNKKVSAWKKLYLIYNFFFIRKIVVHIFTFV 399

Query: 377 FYCVVLPLTILVPEVYVPIWGAVYIPSIITILNSVGTPRSIHLLFYWILFENVMSLHRTK 436
           FYC++LP T+L PE+ VP W  VY P+ ITILN++ TPRS+HLL +WILFENVMS+HRTK
Sbjct: 400 FYCLILPTTVLFPELQVPKWATVYFPTTITILNAIATPRSLHLLVFWILFENVMSMHRTK 459

Query: 437 ATLIGLFEAGRANEWVVTEKLGDALKNKAAADAAKLKANAKVPKIRLRCKLGDRINTLEL 496
           AT IGL EAGR NEWVVTEKLGD LK+K    A            +L  + G R+N  EL
Sbjct: 460 ATFIGLLEAGRVNEWVVTEKLGDTLKSKLIGKAT----------TKLYTRFGQRLNWREL 519

Query: 497 GFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFLITGIGYVGTIIPS 542
               ++F CGCYDF +G + ++VYLFLQ+ +F + G+GY+GT +P+
Sbjct: 520 VVGLYIFFCGCYDFAYGGSYFYVYLFLQSCAFFVAGVGYIGTFVPT 555

BLAST of CmaCh20G005480 vs. TAIR 10
Match: AT1G23480.3 (cellulose synthase-like A3 )

HSP 1 Score: 682.2 bits (1759), Expect = 3.5e-196
Identity = 321/493 (65.11%), Postives = 399/493 (80.93%), Query Frame = 0

Query: 50  MSLMLFFERLYMGIVIILVKLFWKKPEKRYNYEPI-QDDLELGSSNFPHVLIQIPMFNER 109
           MSL+LF ER+YM IV++ VKL  + PEK + +EPI  DDLEL ++N+P VLIQIPM+NE+
Sbjct: 1   MSLLLFIERVYMSIVVVFVKLLRRTPEKVHKWEPINDDDLELANTNYPMVLIQIPMYNEK 60

Query: 110 EVYKISIGAACGLSWPADRLVIQVLDDSTDPVIKQMVEQECLRWASKGINITYQIRETRG 169
           EV ++SIGAAC LSWP DR+++QVLDDSTDP  K++V  EC +WA KGINI  +IR+ R 
Sbjct: 61  EVCQLSIGAACRLSWPLDRMIVQVLDDSTDPASKELVNAECDKWARKGINIMSEIRDNRI 120

Query: 170 GYKAGALKEGLKRSYVKHCEYVAIFDADFRPEPDYLRRAIPFLVHNSDIALVQARWRFVN 229
           GYKAGALK G+  +YVK CE+VAIFDADF+P+PD+L R IPFL+HN +I+LVQ RW+FVN
Sbjct: 121 GYKAGALKAGMMHNYVKQCEFVAIFDADFQPDPDFLERTIPFLIHNHEISLVQCRWKFVN 180

Query: 230 ADECLLTRMQEMSLDYHFTVEQEVGSATHAFFGFNGTAGVWRIAAINEAGGWKDRTTVED 289
           A+ECL+TRMQEMSL+YHF  EQE GS+ HAFFGFNGTAGVWRIAA+NEAGGWKDRTTVED
Sbjct: 181 ANECLMTRMQEMSLNYHFVAEQESGSSIHAFFGFNGTAGVWRIAALNEAGGWKDRTTVED 240

Query: 290 MDLAVRASLRGWKFVYLGDLQVKSELPSTFKAFRFQQHRWSCGPANLFRKMVMEIVRNKK 349
           MDLAVRA L GWKFVY+ D++VK+ELPSTFKA+RFQQHRWSCGPANL+RKM MEI++NKK
Sbjct: 241 MDLAVRACLHGWKFVYVHDVEVKNELPSTFKAYRFQQHRWSCGPANLWRKMTMEILQNKK 300

Query: 350 VKFWKKVYVIYSFFFVRKIIAHMVTFFFYCVVLPLTILVPEVYVPIWGAVYIPSIITILN 409
           V  WKK+Y+IY+FFF+RKI+ H+ TF FYC++LP T+L PE+ VP W  VY P+ ITILN
Sbjct: 301 VSAWKKLYLIYNFFFIRKIVVHIFTFVFYCLILPTTVLFPELQVPKWATVYFPTTITILN 360

Query: 410 SVGTPRSIHLLFYWILFENVMSLHRTKATLIGLFEAGRANEWVVTEKLGDALKNKAAADA 469
           ++ TPRS+HLL +WILFENVMS+HRTKAT IGL EAGR NEWVVTEKLGD LK+K    A
Sbjct: 361 AIATPRSLHLLVFWILFENVMSMHRTKATFIGLLEAGRVNEWVVTEKLGDTLKSKLIGKA 420

Query: 470 AKLKANAKVPKIRLRCKLGDRINTLELGFAAFLFLCGCYDFVHGKNCYFVYLFLQTFSFL 529
                       +L  + G R+N  EL    ++F CGCYDF +G + ++VYLFLQ+ +F 
Sbjct: 421 T----------TKLYTRFGQRLNWRELVVGLYIFFCGCYDFAYGGSYFYVYLFLQSCAFF 480

Query: 530 ITGIGYVGTIIPS 542
           + G+GY+GT +P+
Sbjct: 481 VAGVGYIGTFVPT 483

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FNI75.3e-25882.28Glucomannan 4-beta-mannosyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=CSLA... [more]
Q9LZR32.0e-22570.41Glucomannan 4-beta-mannosyltransferase 9 OS=Arabidopsis thaliana OX=3702 GN=CSLA... [more]
Q7PC763.2e-22374.03Glucomannan 4-beta-mannosyltransferase 1 OS=Oryza sativa subsp. japonica OX=3994... [more]
Q67VS75.4e-21869.45Probable glucomannan 4-beta-mannosyltransferase 9 OS=Oryza sativa subsp. japonic... [more]
Q9LQC94.3e-19962.74Probable glucomannan 4-beta-mannosyltransferase 3 OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A6J1JDM30.0e+00100.00glucomannan 4-beta-mannosyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FXT30.0e+0099.45glucomannan 4-beta-mannosyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A1S3C7U54.3e-30396.86glucomannan 4-beta-mannosyltransferase 2 OS=Cucumis melo OX=3656 GN=LOC103497977... [more]
A0A6J1JZW13.1e-30195.57glucomannan 4-beta-mannosyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1H3253.1e-30195.57glucomannan 4-beta-mannosyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
XP_022986280.10.0e+00100.00glucomannan 4-beta-mannosyltransferase 2-like [Cucurbita maxima][more]
XP_022944193.10.0e+0099.45glucomannan 4-beta-mannosyltransferase 2-like [Cucurbita moschata] >KAG6570842.1... [more]
XP_023522032.11.1e-30597.23glucomannan 4-beta-mannosyltransferase 2-like [Cucurbita pepo subsp. pepo][more]
XP_008458630.18.9e-30396.86PREDICTED: glucomannan 4-beta-mannosyltransferase 2 [Cucumis melo][more]
XP_038901424.13.4e-30296.31glucomannan 4-beta-mannosyltransferase 2 [Benincasa hispida] >XP_038901425.1 glu... [more]
Match NameE-valueIdentityDescription
AT5G22740.13.8e-25982.28cellulose synthase-like A02 [more]
AT5G03760.11.4e-22670.41Nucleotide-diphospho-sugar transferases superfamily protein [more]
AT1G23480.13.0e-20062.74cellulose synthase-like A3 [more]
AT1G23480.23.0e-20062.74cellulose synthase-like A3 [more]
AT1G23480.33.5e-19665.11cellulose synthase-like A3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001173Glycosyltransferase 2-likePFAMPF13632Glyco_trans_2_3coord: 189..399
e-value: 3.6E-22
score: 79.2
IPR029044Nucleotide-diphospho-sugar transferasesGENE3D3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain Acoord: 87..315
e-value: 1.5E-62
score: 212.8
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 93..377
NoneNo IPR availablePANTHERPTHR32044:SF92BNAC09G36340D PROTEINcoord: 7..541
NoneNo IPR availablePANTHERPTHR32044FAMILY NOT NAMEDcoord: 7..541
NoneNo IPR availableCDDcd06437CESA_CaSu_A2coord: 96..332
e-value: 6.38872E-142
score: 407.081

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G005480.1CmaCh20G005480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0097502 mannosylation
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051753 mannan synthase activity