CmaCh20G004330 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh20G004330
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like
Location	Cma_Chr20: 2048378 .. 2049697 (-)
RNA-Seq Expression	CmaCh20G004330
Synteny	CmaCh20G004330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGGGTCCCACCCCTCTTCTTCTTCCTCCTCCTCCTCCTCCTCTCTCTTTGCTTAAAACCAAAATGGTTGCTCTCATTTCATCACATATTTCAATCCCAACATGAAAAAATTGGACTTTTTCTGCAAATCCAGAGCTTCCACGGCGGTTCGTTCAAGTTTCGGTCGTCGTCCGGTTGACGGAGATGCAAATTCCGGCGACCGGAGGAAATGGCAGCTTCCTTTTGAGAATCACAGAAAGAGTACGAGCTGTTCTTCTGTGAATCGGAAGGATCATTATGATTTACGAAGAAAAAGCTGCGCAGATGTGGATGATTTGAAGAGCTCTGTTTCTGGGTCCTCTGCTCGTTATCTCTTGAGTGATTCATCGTTCATTGACTGGTTACCGGCGGTCTCCGGCGAGGATTTTCCGGCGAAACCGCCGAACGAGAGGAACATGATAAGCCATAGTTCGCTGACTCGATCGTTGACAGTCCATGAATATCGTGGCCAAAAATCCCCTTCCTCTGTTCTTCAATCCCCTGTTTTGAAAACAGCTTCTTCGGCTGTTCATGATCAGGTTTTGTTCTTTTTAAGAATAATTTCTTGTCCTTTTCGTTCTAATTGGTCGGGATTTTAAAATTTTCTTTCATGATTCAAAAGGTTGTGGTTCTTAAGGTCTCATTGAACTGCAAAGGCTGCGAAAAAAAGGTGAAAAAACACATCTCCAAGATGGAAGGTAATGGATTGAATTCGACAAAGCTGTTCTTCTTTGCATTTTGAGCTAAAATGTTCATATAGTGTTGATGATCATGCAGGAGTTAGCTCATATAGTGTCGATTTCACAGCAAAAAAAGTGACCATAATTGGAGCAGTGAGCCCTTTTGATGTCTTAGCCAGTGTGTCAAAGGTTAAGTATGCTCAGTTCTGGACATCGCCGAGTTCTTCTTCGTGTTCGTCGTCTTCTTCATCTTCAACTCCGCAATCCTGTTCGTCATCGACATTTTGATCAATGGAGCTATTTGCTGCAAAAATCCCATCTGGGTTTTGCTTGTATTATGCAGAAAAGTTGCAGAATTGATGCTTTTCTTTCTTCTTTTTCTGTTTTGGGTGTAAATTTTGCTGTTTTTGTGATGAAGTTGCAGTAGTAATTCTGTGTTTCCCTGTGGTTTAGTTTGGGGAATAAAGAAAAATGTTTTTTCACTGTTTTCGGGGTTAAATCCTTCCAAATTTGACGTTTTGTGTAGCTACTTCTAACGACTATATGTAGCTCATCGTCTTAGAGAAACTATTCGATGAGACTGCTGCAATGTAAACATATACTTCTAACGACTATATGTAGC

mRNA sequence

GTGGGTCCCACCCCTCTTCTTCTTCCTCCTCCTCCTCCTCCTCTCTCTTTGCTTAAAACCAAAATGGTTGCTCTCATTTCATCACATATTTCAATCCCAACATGAAAAAATTGGACTTTTTCTGCAAATCCAGAGCTTCCACGGCGGTTCGTTCAAGTTTCGGTCGTCGTCCGGTTGACGGAGATGCAAATTCCGGCGACCGGAGGAAATGGCAGCTTCCTTTTGAGAATCACAGAAAGAGTACGAGCTGTTCTTCTGTGAATCGGAAGGATCATTATGATTTACGAAGAAAAAGCTGCGCAGATGTGGATGATTTGAAGAGCTCTGTTTCTGGGTCCTCTGCTCGTTATCTCTTGAGTGATTCATCGTTCATTGACTGGTTACCGGCGGTCTCCGGCGAGGATTTTCCGGCGAAACCGCCGAACGAGAGGAACATGATAAGCCATAGTTCGCTGACTCGATCGTTGACAGTCCATGAATATCGTGGCCAAAAATCCCCTTCCTCTGTTCTTCAATCCCCTGTTTTGAAAACAGCTTCTTCGGCTGTTCATGATCAGGTTGTGGTTCTTAAGGTCTCATTGAACTGCAAAGGCTGCGAAAAAAAGGTGAAAAAACACATCTCCAAGATGGAAGGAGTTAGCTCATATAGTGTCGATTTCACAGCAAAAAAAGTGACCATAATTGGAGCAGTGAGCCCTTTTGATGTCTTAGCCAGTGTGTCAAAGGTTAAGTATGCTCAGTTCTGGACATCGCCGAGTTCTTCTTCGTGTTCGTCGTCTTCTTCATCTTCAACTCCGCAATCCTGTTCGTCATCGACATTTTGATCAATGGAGCTATTTGCTGCAAAAATCCCATCTGGGTTTTGCTTGTATTATGCAGAAAAGTTGCAGAATTGATGCTTTTCTTTCTTCTTTTTCTGTTTTGGGTGTAAATTTTGCTGTTTTTGTGATGAAGTTGCAGTAGTAATTCTGTGTTTCCCTGTGGTTTAGTTTGGGGAATAAAGAAAAATGTTTTTTCACTGTTTTCGGGGTTAAATCCTTCCAAATTTGACGTTTTGTGTAGCTACTTCTAACGACTATATGTAGCTCATCGTCTTAGAGAAACTATTCGATGAGACTGCTGCAATGTAAACATATACTTCTAACGACTATATGTAGC

Coding sequence (CDS)

ATGAAAAAATTGGACTTTTTCTGCAAATCCAGAGCTTCCACGGCGGTTCGTTCAAGTTTCGGTCGTCGTCCGGTTGACGGAGATGCAAATTCCGGCGACCGGAGGAAATGGCAGCTTCCTTTTGAGAATCACAGAAAGAGTACGAGCTGTTCTTCTGTGAATCGGAAGGATCATTATGATTTACGAAGAAAAAGCTGCGCAGATGTGGATGATTTGAAGAGCTCTGTTTCTGGGTCCTCTGCTCGTTATCTCTTGAGTGATTCATCGTTCATTGACTGGTTACCGGCGGTCTCCGGCGAGGATTTTCCGGCGAAACCGCCGAACGAGAGGAACATGATAAGCCATAGTTCGCTGACTCGATCGTTGACAGTCCATGAATATCGTGGCCAAAAATCCCCTTCCTCTGTTCTTCAATCCCCTGTTTTGAAAACAGCTTCTTCGGCTGTTCATGATCAGGTTGTGGTTCTTAAGGTCTCATTGAACTGCAAAGGCTGCGAAAAAAAGGTGAAAAAACACATCTCCAAGATGGAAGGAGTTAGCTCATATAGTGTCGATTTCACAGCAAAAAAAGTGACCATAATTGGAGCAGTGAGCCCTTTTGATGTCTTAGCCAGTGTGTCAAAGGTTAAGTATGCTCAGTTCTGGACATCGCCGAGTTCTTCTTCGTGTTCGTCGTCTTCTTCATCTTCAACTCCGCAATCCTGTTCGTCATCGACATTTTGA

Protein sequence

MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYDLRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTRSLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVSSYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF

Homology

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BLAST of CmaCh20G004330 vs. ExPASy Swiss-Prot
Match: Q8LDS4 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAKR1 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-20
Identity = 59/131 (45.04%), Postives = 85/131 (64.89%), Query Frame = 0


Query: 90  FIDWLPAVSGEDFPAKPPNERNMISHSSL-TRSLTVHEYR----GQKSPSSVLQSPVLKT 149
           F+D+ P +S ++  ++P       S SSL  + ++  +++        PS    SP    
Sbjct: 183 FLDYEPVLSPDNPFSEPTKASPTASLSSLEDKDVSSPDFKFSPPPPPPPSPPQSSPPSPP 242

Query: 150 ASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVSSYSVDFTAKKVTIIGAVSPFDVLA 209
             ++  DQVVVL+VSL+CKGC  KVKKH+SK++GV+SY++DF AKKVT+ G V+P  VLA
Sbjct: 243 EKNSSSDQVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVTPLTVLA 302

Query: 210 SVSKVKYAQFW 216
           S+SKVK AQFW
Sbjct: 303 SISKVKNAQFW 313

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BLAST of CmaCh20G004330 vs. ExPASy Swiss-Prot
Match: Q58FZ0 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAKR2 PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.0e-19
Identity = 44/77 (57.14%), Postives = 63/77 (81.82%), Query Frame = 0


Query: 143 KTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVSSYSVDFTAKKVTIIGAVSPFDV 202
           KT       QVVVLKVSL+C+GCE KV+KH+++M+GV+S+++DF AKKVT+ G ++P ++
Sbjct: 172 KTTCGDTDQQVVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVTGDITPLEI 231

Query: 203 LASVSKVKYAQFWTSPS 220
           L S+SKVK AQFWT+P+
Sbjct: 232 LDSISKVKNAQFWTNPT 248

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BLAST of CmaCh20G004330 vs. ExPASy Swiss-Prot
Match: Q8RXH8 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAKR3 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.8e-17
Identity = 83/255 (32.55%), Postives = 117/255 (45.88%), Query Frame = 0


Query: 1   MKKLDFFCKSRASTAVRS-----------------SFGRRPVDGDANSGDRRKWQLPFEN 60
           MK   F+C S+ASTA  +                   GRR      +SGD   +  P+  
Sbjct: 1   MKAGMFYCASQASTATANGERTVTARAIDRHNPIIKDGRRSFTAPCSSGD--DYVAPYRQ 60

Query: 61  HRKSTSCSSVNRKDHYDLRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDF- 120
             K T   S +              VD  + S SGS  + +  D S       ++ + F 
Sbjct: 61  LSKITRVPSSSGDG-------KSVQVDKGRRSNSGSLMKLISYDVS-------LARKSFG 120

Query: 121 -----PAKPPNERNMISHS-------SLTRSLTVHEYRGQKSPSSVLQSPV--LKTASSA 180
                P  PP     +  S       S  +     E     +P      PV   K +S +
Sbjct: 121 CVVATPKTPPGSTRYLLGSDPVSLAGSTGQDTVATEESEASAPKRGSSGPVEEKKKSSGS 180

Query: 181 VHDQVVVLKVSL--NCKGCEKKVKKHISKMEGVSSYSVDFTAKKVTIIGAVSPFDVLASV 222
             DQVVVL+VSL  +C+GC+ KVKKH+SKM+GV+S+++DF +KKVT+ G ++P +VL  +
Sbjct: 181 GSDQVVVLRVSLHCHCRGCQGKVKKHLSKMQGVTSFNIDFASKKVTVTGDITPLEVLGCL 239

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BLAST of CmaCh20G004330 vs. ExPASy Swiss-Prot
Match: B3H6D0 (Heavy metal-associated isoprenylated plant protein 45 OS=Arabidopsis thaliana OX=3702 GN=HIPP45 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.3e-06
Identity = 28/67 (41.79%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 153 VVVLKVSLNCKGCEKKVKKHISKMEGVSSYSVDFTAKKVTIIGAVSPFDVLASVSKV-KY 212
           +V L V ++CKGCEKKV++ ISK++GV +  +D   +KVT+ G V   +VL  V +  + 
Sbjct: 17  IVELLVDMDCKGCEKKVRRAISKLDGVDTVEIDVDRQKVTVTGYVDREEVLKMVKRTGRT 76

Query: 213 AQFWTSP 219
           A++W  P
Sbjct: 77  AEYWPFP 83

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BLAST of CmaCh20G004330 vs. ExPASy Swiss-Prot
Match: Q94BT9 (Copper transport protein ATX1 OS=Arabidopsis thaliana OX=3702 GN=ATX1 PE=1 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 2.2e-06
Identity = 31/72 (43.06%), Postives = 45/72 (62.50%), Query Frame = 0


Query: 152 QVVVLKVSLNCKGCEKKVKKHISKMEGVSSYSVDFTAKKVTIIGAVSPFDVLASVSKV-K 211
           Q VVL+V++ C+GC   VK+ + KMEGV S+ VD   +KVT+ G V P  VL +V+K  K
Sbjct: 3   QTVVLRVAMTCEGCVGAVKRVLGKMEGVESFDVDIKEQKVTVKGNVQPDAVLQTVTKTGK 62

Query: 212 YAQFWTSPSSSS 223
              FW +   ++
Sbjct: 63  KTAFWEAEGETA 74

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BLAST of CmaCh20G004330 vs. ExPASy TrEMBL
Match: A0A6J1J8X9 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like OS=Cucurbita maxima OX=3661 GN=LOC111484523 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 9.3e-125
Identity = 240/240 (100.00%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60
           MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD
Sbjct: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60

Query: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120
           LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR
Sbjct: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120

Query: 121 SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180
           SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS
Sbjct: 121 SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180

Query: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF 240
           SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF
Sbjct: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF 240

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BLAST of CmaCh20G004330 vs. ExPASy TrEMBL
Match: A0A6J1FTK0 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like OS=Cucurbita moschata OX=3662 GN=LOC111448670 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.3e-121
Identity = 238/241 (98.76%), Postives = 238/241 (98.76%), Query Frame = 0


Query: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60
           MKKLDFFCKSRASTAVRSSFGRRPVDGD NSGDRRKWQLPFENHRKSTSCSSVNRKDHYD
Sbjct: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDENSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60

Query: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120
           LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR
Sbjct: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120

Query: 121 SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180
           SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS
Sbjct: 121 SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180

Query: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSC-SSSSSSSTPQSCSSST 240
           SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTS SSSSC SSSSSSSTPQSCSSST
Sbjct: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSLSSSSCSSSSSSSSTPQSCSSST 240

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BLAST of CmaCh20G004330 vs. ExPASy TrEMBL
Match: A0A0A0KG24 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G302690 PE=4 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.9e-85
Identity = 185/245 (75.51%), Postives = 203/245 (82.86%), Query Frame = 0


Query: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60
           MKKLD FCKSRASTAVRSSF RRP+ GDA+SGDRRK QL FENHRKSTSCS++NRK+  D
Sbjct: 1   MKKLDIFCKSRASTAVRSSFARRPLTGDAHSGDRRKGQLHFENHRKSTSCSTLNRKELND 60

Query: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSS--- 120
           LRRKSCADVDDLKS VSGSSARYLL DS F+DW PAVSGE+ PA  P +R +IS +S   
Sbjct: 61  LRRKSCADVDDLKSPVSGSSARYLLGDSPFLDWFPAVSGEEVPALMPEKRKIISDNSQKS 120

Query: 121 --LTRSLTVHEYRGQKSPSSVLQSPVLKTAS-SAVHDQVVVLKVSLNCKGCEKKVKKHIS 180
             L RSLTV EY G KSPSSVL+SPVLKT S +   DQVVVLKVSLNC+GCEKKVKKHIS
Sbjct: 121 FLLNRSLTVREYGGLKSPSSVLESPVLKTPSLTQSRDQVVVLKVSLNCRGCEKKVKKHIS 180

Query: 181 KMEGVSSYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQS 240
           KMEGV+SYSVDFT KKVTIIG ++PFDVLASVSKVK AQFW SP      +SSSSSTPQS
Sbjct: 181 KMEGVTSYSVDFTTKKVTIIGDITPFDVLASVSKVKSAQFWPSP------NSSSSSTPQS 239

[Close]

BLAST of CmaCh20G004330 vs. ExPASy TrEMBL
Match: A0A6J1D3S6 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017000 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 2.6e-82
Identity = 183/243 (75.31%), Postives = 194/243 (79.84%), Query Frame = 0


Query: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60
           MK LD FCKSRASTAV SS  RRPV     SG+RRK QL FE HRKSTSCSS NRKD ++
Sbjct: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGYSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60

Query: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120
            RRKSCADVDDLKS  SGSSARYLL DS FIDW PA SGEDFPA PP ER   S SSLTR
Sbjct: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKS-SSLTR 120

Query: 121 SLTVHEYRGQKSPSSVL--QSPVLKTASSA-VHDQVVVLKVSLNCKGCEKKVKKHISKME 180
           SLT HEYRG KSPSSVL  +SPVLK+ SSA    QVVVLKVSLNCKGCEKKVKKHISKME
Sbjct: 121 SLTAHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKME 180

Query: 181 GVSSYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSS 240
           GV+SYSVDFT KKVTIIG V+PF VL SVSKVK+AQ W SP+SS+ SSSSSS+   S SS
Sbjct: 181 GVTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPKSSASS 240

[Close]

BLAST of CmaCh20G004330 vs. ExPASy TrEMBL
Match: A0A6J1D3N2 (protein SODIUM POTASSIUM ROOT DEFECTIVE 2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017000 PE=4 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 4.5e-79
Identity = 177/242 (73.14%), Postives = 189/242 (78.10%), Query Frame = 0


Query: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60
           MK LD FCKSRASTAV SS  RRPV     SG+RRK QL FE HRKSTSCSS NRKD ++
Sbjct: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGYSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60

Query: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120
            RRKSCADVDDLKS  SGSSARYLL DS FIDW PA SGEDFPA PP ER   S SSLTR
Sbjct: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKS-SSLTR 120

Query: 121 SLTVHEYRGQKSPSSVL--QSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEG 180
           SLT HEYRG KSPSSVL  +SPVLK+ SSA        +VSLNCKGCEKKVKKHISKMEG
Sbjct: 121 SLTAHEYRGLKSPSSVLSHESPVLKSPSSAQSRH----QVSLNCKGCEKKVKKHISKMEG 180

Query: 181 VSSYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSS 240
           V+SYSVDFT KKVTIIG V+PF VL SVSKVK+AQ W SP+SS+ SSSSSS+   S SS 
Sbjct: 181 VTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPKSSASSP 237

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BLAST of CmaCh20G004330 vs. NCBI nr
Match: XP_022986932.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like [Cucurbita maxima])

HSP 1 Score: 456.1 bits (1172), Expect = 1.9e-124
Identity = 240/240 (100.00%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60
           MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD
Sbjct: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60

Query: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120
           LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR
Sbjct: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120

Query: 121 SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180
           SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS
Sbjct: 121 SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180

Query: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF 240
           SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF
Sbjct: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF 240

[Close]

BLAST of CmaCh20G004330 vs. NCBI nr
Match: KAG6570732.1 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 453.8 bits (1166), Expect = 9.5e-124
Identity = 239/240 (99.58%), Postives = 239/240 (99.58%), Query Frame = 0


Query: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60
           MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD
Sbjct: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60

Query: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120
           LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPP ERNMISHSSLTR
Sbjct: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPKERNMISHSSLTR 120

Query: 121 SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180
           SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS
Sbjct: 121 SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180

Query: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF 240
           SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF
Sbjct: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF 240

[Close]

BLAST of CmaCh20G004330 vs. NCBI nr
Match: KAG7010577.1 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 451.4 bits (1160), Expect = 4.7e-123
Identity = 238/240 (99.17%), Postives = 238/240 (99.17%), Query Frame = 0


Query: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60
           MKKLDFFCKSRASTAVRSSFGRRPVDGDAN GDRRKWQLPFENHRKSTSCSSVNRKDHYD
Sbjct: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANFGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60

Query: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120
           LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPP ERNMISHSSLTR
Sbjct: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPKERNMISHSSLTR 120

Query: 121 SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180
           SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS
Sbjct: 121 SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180

Query: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF 240
           SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF
Sbjct: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF 240

[Close]

BLAST of CmaCh20G004330 vs. NCBI nr
Match: XP_023512005.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 449.1 bits (1154), Expect = 2.3e-122
Identity = 237/240 (98.75%), Postives = 237/240 (98.75%), Query Frame = 0


Query: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60
           MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD
Sbjct: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60

Query: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120
           LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR
Sbjct: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120

Query: 121 SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180
           SLTVHEYR QKSPSSVLQSPVLKTASS VHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS
Sbjct: 121 SLTVHEYRAQKSPSSVLQSPVLKTASSTVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180

Query: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSSSTF 240
           SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTS SSSSCSSSSSSSTPQSCSSSTF
Sbjct: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSTSSSSCSSSSSSSTPQSCSSSTF 240

[Close]

BLAST of CmaCh20G004330 vs. NCBI nr
Match: XP_022944121.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like [Cucurbita moschata])

HSP 1 Score: 445.7 bits (1145), Expect = 2.6e-121
Identity = 238/241 (98.76%), Postives = 238/241 (98.76%), Query Frame = 0


Query: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60
           MKKLDFFCKSRASTAVRSSFGRRPVDGD NSGDRRKWQLPFENHRKSTSCSSVNRKDHYD
Sbjct: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDENSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60

Query: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120
           LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR
Sbjct: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120

Query: 121 SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180
           SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS
Sbjct: 121 SLTVHEYRGQKSPSSVLQSPVLKTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVS 180

Query: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSC-SSSSSSSTPQSCSSST 240
           SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTS SSSSC SSSSSSSTPQSCSSST
Sbjct: 181 SYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSLSSSSCSSSSSSSSTPQSCSSST 240

[Close]

BLAST of CmaCh20G004330 vs. TAIR 10
Match: AT2G28660.1 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 122.5 bits (306), Expect = 4.7e-28
Identity = 96/259 (37.07%), Postives = 129/259 (49.81%), Query Frame = 0


Query: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRR-----KWQLPFENHRKSTSCSSVN- 60
           M+  D FC S ASTAVR S      DG+   G R      + Q P +   K+  C S   
Sbjct: 1   MRFSDIFCSSPASTAVRHSTLHH--DGNVTGGRRSFQSHLRSQNPSKKKDKTVPCFSSEM 60

Query: 61  ----------------------------RKDHYDLRRKSCADVDDL---KSSVSGSSARY 120
                                       R     +RRKS ADV DL   +SS+  SS+RY
Sbjct: 61  PLIPIPRHLSCRNSFESPSGFRPKIASARGSDVQIRRKSSADVSDLRRSRSSLLSSSSRY 120

Query: 121 LLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTRSLTVHEYRGQKSPSSVLQSPVLK 180
           LL D        ++ G+D      ++R+        R++T        S SS   S V  
Sbjct: 121 LLKDHK------SLKGDDKDLWLSSDRSKDLILYRDRNVTSSASSSSSSSSSSFSSSVTN 180

Query: 181 TASSA--VHDQVVVLKVSLNCKGCEKKVKKHISKMEGVSSYSVDFTAKKVTIIGAVSPFD 221
            +S A    DQVVVL+VS++CKGCE KV+KHISKMEGV+SY++D   KKVT++G ++P  
Sbjct: 181 VSSPAPSTDDQVVVLRVSIHCKGCEGKVRKHISKMEGVTSYTIDLATKKVTVVGKITPVG 240

[Close]

BLAST of CmaCh20G004330 vs. TAIR 10
Match: AT5G02600.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 101.7 bits (252), Expect = 8.6e-22
Identity = 59/131 (45.04%), Postives = 85/131 (64.89%), Query Frame = 0


Query: 90  FIDWLPAVSGEDFPAKPPNERNMISHSSL-TRSLTVHEYR----GQKSPSSVLQSPVLKT 149
           F+D+ P +S ++  ++P       S SSL  + ++  +++        PS    SP    
Sbjct: 183 FLDYEPVLSPDNPFSEPTKASPTASLSSLEDKDVSSPDFKFSPPPPPPPSPPQSSPPSPP 242

Query: 150 ASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVSSYSVDFTAKKVTIIGAVSPFDVLA 209
             ++  DQVVVL+VSL+CKGC  KVKKH+SK++GV+SY++DF AKKVT+ G V+P  VLA
Sbjct: 243 EKNSSSDQVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVTPLTVLA 302

Query: 210 SVSKVKYAQFW 216
           S+SKVK AQFW
Sbjct: 303 SISKVKNAQFW 313

[Close]

BLAST of CmaCh20G004330 vs. TAIR 10
Match: AT5G02600.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 101.7 bits (252), Expect = 8.6e-22
Identity = 59/131 (45.04%), Postives = 85/131 (64.89%), Query Frame = 0


Query: 90  FIDWLPAVSGEDFPAKPPNERNMISHSSL-TRSLTVHEYR----GQKSPSSVLQSPVLKT 149
           F+D+ P +S ++  ++P       S SSL  + ++  +++        PS    SP    
Sbjct: 183 FLDYEPVLSPDNPFSEPTKASPTASLSSLEDKDVSSPDFKFSPPPPPPPSPPQSSPPSPP 242

Query: 150 ASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVSSYSVDFTAKKVTIIGAVSPFDVLA 209
             ++  DQVVVL+VSL+CKGC  KVKKH+SK++GV+SY++DF AKKVT+ G V+P  VLA
Sbjct: 243 EKNSSSDQVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVTPLTVLA 302

Query: 210 SVSKVKYAQFW 216
           S+SKVK AQFW
Sbjct: 303 SISKVKNAQFW 313

[Close]

BLAST of CmaCh20G004330 vs. TAIR 10
Match: AT2G37390.2 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.6e-20
Identity = 43/69 (62.32%), Postives = 62/69 (89.86%), Query Frame = 0


Query: 151 DQVVVLKVSLNCKGCEKKVKKHISKMEGVSSYSVDFTAKKVTIIGAVSPFDVLASVSKVK 210
           DQVVVLKVSL+C+GCE KV+KH+++M+GV+S+++DF AKKVT+ G ++P ++L S+SKVK
Sbjct: 179 DQVVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVTGDITPLEILDSISKVK 238

Query: 211 YAQFWTSPS 220
            AQFWT+P+
Sbjct: 239 NAQFWTNPT 247

[Close]

BLAST of CmaCh20G004330 vs. TAIR 10
Match: AT2G37390.1 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 97.1 bits (240), Expect = 2.1e-20
Identity = 44/77 (57.14%), Postives = 63/77 (81.82%), Query Frame = 0


Query: 143 KTASSAVHDQVVVLKVSLNCKGCEKKVKKHISKMEGVSSYSVDFTAKKVTIIGAVSPFDV 202
           KT       QVVVLKVSL+C+GCE KV+KH+++M+GV+S+++DF AKKVT+ G ++P ++
Sbjct: 172 KTTCGDTDQQVVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVTGDITPLEI 231

Query: 203 LASVSKVKYAQFWTSPS 220
           L S+SKVK AQFWT+P+
Sbjct: 232 LDSISKVKNAQFWTNPT 248

The following BLAST results are available for this feature:

BLAST of CmaCh20G004330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LDS4	1.2e-20	45.04	Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAK...	[more]
Q58FZ0	3.0e-19	57.14	Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAK...	[more]
Q8RXH8	2.8e-17	32.55	Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAK...	[more]
B3H6D0	1.3e-06	41.79	Heavy metal-associated isoprenylated plant protein 45 OS=Arabidopsis thaliana OX...	[more]
Q94BT9	2.2e-06	43.06	Copper transport protein ATX1 OS=Arabidopsis thaliana OX=3702 GN=ATX1 PE=1 SV=2	[more]

BLAST of CmaCh20G004330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J8X9	9.3e-125	100.00	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1FTK0	1.3e-121	98.76	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A0A0KG24	1.9e-85	75.51	HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G302690 PE=4 SV...	[more]
A0A6J1D3S6	2.6e-82	75.31	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like isoform X1 OS=Momordica charantia...	[more]
A0A6J1D3N2	4.5e-79	73.14	protein SODIUM POTASSIUM ROOT DEFECTIVE 2-like isoform X2 OS=Momordica charantia...	[more]

BLAST of CmaCh20G004330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022986932.1	1.9e-124	100.00	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like [Cucurbita maxima]	[more]
KAG6570732.1	9.5e-124	99.58	Protein SODIUM POTASSIUM ROOT DEFECTIVE 2, partial [Cucurbita argyrosperma subsp...	[more]
KAG7010577.1	4.7e-123	99.17	Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 [Cucurbita argyrosperma subsp. argyros...	[more]
XP_023512005.1	2.3e-122	98.75	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022944121.1	2.6e-121	98.76	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like [Cucurbita moschata]	[more]

BLAST of CmaCh20G004330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28660.1	4.7e-28	37.07	Chloroplast-targeted copper chaperone protein	[more]
AT5G02600.2	8.6e-22	45.04	Heavy metal transport/detoxification superfamily protein	[more]
AT5G02600.1	8.6e-22	45.04	Heavy metal transport/detoxification superfamily protein	[more]
AT2G37390.2	1.6e-20	62.32	Chloroplast-targeted copper chaperone protein	[more]
AT2G37390.1	2.1e-20	57.14	Chloroplast-targeted copper chaperone protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.70.100		coord: 149..213 e-value: 2.0E-15 score: 58.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 216..240
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 20..39
None	No IPR available	PANTHER	PTHR46119:SF12	HEAVY METAL-ASSOCIATED DOMAIN CONTAINING PROTEIN, EXPRESSED	coord: 1..229
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 157..211 e-value: 3.3E-12 score: 46.6
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 152..215 score: 10.785673
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 155..211 e-value: 1.41669E-11 score: 56.4601
IPR044526	Protein SODIUM POTASSIUM ROOT DEFECTIVE 1/2/3	PANTHER	PTHR46119	OS08G0405700 PROTEIN	coord: 1..229
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 152..212

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh20G004330.1	CmaCh20G004330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding