CmaCh20G004320 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh20G004320
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	RING-type E3 ubiquitin transferase
Location	Cma_Chr20: 2043801 .. 2044442 (+)
RNA-Seq Expression	CmaCh20G004320
Synteny	CmaCh20G004320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCATCAAGTCCGCCACCGCCGCCGCCGCTATCTCCGGTACCTGATTACTCTAGCTGGAGTCCATGGTCAATCTCGATCATCGCCATCGTATGCATCGTCGTCCTACTGTTGAGCAACTACAGAATATTGAAACGATGGTGTCGTGTCCTTCATGAATTGTTTTGCGGTCGATCTGTTGTCCAATTCGATGAAAACCCTAACGATCCTTCTCCGCAAATGCATGTCCATGGATTGGAGGCCTCGACGATTCGTTTGCTACCTATATCACAGTTCAAAAAGAACGAAGAAGCCGAATCAACGGCAAGCATCAATACAGAATGCGCTGTTTGTTTGGGCGAATTCGAAGAAGGCGAAATGATTAAAAACTTACCGAATTGCAATCATTCGTTTCATAGTTCTTGCATCGACGCCTGGCTCCGTAACCATTCGAGTTGCCCTCTCTGCAGATTGCACGTCTCGAATCTCTCTATCAATTGTCCTACTCCTGATTCTTCTTCAGCAATGCTGGAGACGCTTGGTAGGGAAGACGTTGACGGAGAAAGAGTATCGTATTATCAAACACTTCGTGCTCAAATTCTTCAGAATCCAGAGTTGAGAAGAGATGCTTCGGTTCAAGGATCCGATCAACGAAACCGATGA

mRNA sequence

ATGCCATCAAGTCCGCCACCGCCGCCGCCGCTATCTCCGGTACCTGATTACTCTAGCTGGAGTCCATGGTCAATCTCGATCATCGCCATCGTATGCATCGTCGTCCTACTGTTGAGCAACTACAGAATATTGAAACGATGGTGTCGTGTCCTTCATGAATTGTTTTGCGGTCGATCTGTTGTCCAATTCGATGAAAACCCTAACGATCCTTCTCCGCAAATGCATGTCCATGGATTGGAGGCCTCGACGATTCGTTTGCTACCTATATCACAGTTCAAAAAGAACGAAGAAGCCGAATCAACGGCAAGCATCAATACAGAATGCGCTGTTTGTTTGGGCGAATTCGAAGAAGGCGAAATGATTAAAAACTTACCGAATTGCAATCATTCGTTTCATAGTTCTTGCATCGACGCCTGGCTCCGTAACCATTCGAGTTGCCCTCTCTGCAGATTGCACGTCTCGAATCTCTCTATCAATTGTCCTACTCCTGATTCTTCTTCAGCAATGCTGGAGACGCTTGGTAGGGAAGACGTTGACGGAGAAAGAGTATCGTATTATCAAACACTTCGTGCTCAAATTCTTCAGAATCCAGAGTTGAGAAGAGATGCTTCGGTTCAAGGATCCGATCAACGAAACCGATGA

Coding sequence (CDS)

ATGCCATCAAGTCCGCCACCGCCGCCGCCGCTATCTCCGGTACCTGATTACTCTAGCTGGAGTCCATGGTCAATCTCGATCATCGCCATCGTATGCATCGTCGTCCTACTGTTGAGCAACTACAGAATATTGAAACGATGGTGTCGTGTCCTTCATGAATTGTTTTGCGGTCGATCTGTTGTCCAATTCGATGAAAACCCTAACGATCCTTCTCCGCAAATGCATGTCCATGGATTGGAGGCCTCGACGATTCGTTTGCTACCTATATCACAGTTCAAAAAGAACGAAGAAGCCGAATCAACGGCAAGCATCAATACAGAATGCGCTGTTTGTTTGGGCGAATTCGAAGAAGGCGAAATGATTAAAAACTTACCGAATTGCAATCATTCGTTTCATAGTTCTTGCATCGACGCCTGGCTCCGTAACCATTCGAGTTGCCCTCTCTGCAGATTGCACGTCTCGAATCTCTCTATCAATTGTCCTACTCCTGATTCTTCTTCAGCAATGCTGGAGACGCTTGGTAGGGAAGACGTTGACGGAGAAAGAGTATCGTATTATCAAACACTTCGTGCTCAAATTCTTCAGAATCCAGAGTTGAGAAGAGATGCTTCGGTTCAAGGATCCGATCAACGAAACCGATGA

Protein sequence

MPSSPPPPPPLSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHELFCGRSVVQFDENPNDPSPQMHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEMIKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPTPDSSSAMLETLGREDVDGERVSYYQTLRAQILQNPELRRDASVQGSDQRNR

Homology

[Close]

BLAST of CmaCh20G004320 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.1e-20
Identity = 68/185 (36.76%), Postives = 99/185 (53.51%), Query Frame = 0


Query: 5   PPPPPPL--SPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHEL-FCGR-SV 64
           PPPPPP+        +S+   ++++I I+    LL+S Y  + + C   H +   GR S+
Sbjct: 17  PPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSL 76

Query: 65  VQFDENPNDP----SPQMHVHGLEASTIRLLPISQFKK----------NEEAESTASINT 124
            +   N  DP    SP++   GL+ S IR +PI +FKK           E  E     + 
Sbjct: 77  SRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQ 136

Query: 125 ECAVCLGEFEEGEMIKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPT---P 169
           EC+VCL EF++ E ++ +PNC+H FH  CID WL+N+++CPLCR  VS     C T   P
Sbjct: 137 ECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVS-----CDTSFPP 196

[Close]

BLAST of CmaCh20G004320 vs. ExPASy Swiss-Prot
Match: P93823 (RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.3e-18
Identity = 63/164 (38.41%), Postives = 90/164 (54.88%), Query Frame = 0


Query: 5   PPPPPP----LSPVPDYSSWSPWSISIIAIVCIVV-----LLLSNYRILKRWCRVLHEL- 64
           PPPPPP     SP+   +S S  +  I+AI  I +     LL+S Y  + + C   H++ 
Sbjct: 17  PPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQID 76

Query: 65  FCGRSVVQFDENP-NDPSPQMHVHGLEASTIRLLPISQFKKNEEA---ESTASINTECAV 124
              R     D+NP    SP     GL+ S IR +P+ +FKK +     E  +  + EC+V
Sbjct: 77  IFRRRRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNSQECSV 136

Query: 125 CLGEFEEGEMIKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVS 155
           CL EF+E E ++ +PNC H FH  CID WL+ +++CPLCR  VS
Sbjct: 137 CLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVS 180

[Close]

BLAST of CmaCh20G004320 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.3e-18
Identity = 56/166 (33.73%), Postives = 89/166 (53.61%), Query Frame = 0


Query: 6   PPPPPLSPVPD-----YSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHELFCGRSV 65
           PPPPP   + D      SS+SP  I++I I+   ++L+S Y ++ ++C   H+     ++
Sbjct: 35  PPPPPSFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETL 94

Query: 66  VQFDENPNDPSPQMHV----HGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFE 125
                     S    +     GL  S I+ + + ++K  +         ++C+VCL EFE
Sbjct: 95  NLNHNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVD----GSDCSVCLSEFE 154

Query: 126 EGEMIKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPT 163
           E E ++ LP CNH+FH  CID WL++HS+CPLCR  V+   +N PT
Sbjct: 155 ENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFVT--GVNNPT 194

[Close]

BLAST of CmaCh20G004320 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 91.7 bits (226), Expect = 1.1e-17
Identity = 57/183 (31.15%), Postives = 93/183 (50.82%), Query Frame = 0


Query: 6   PPPPPLSPV---PDYSS---WSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHEL---FC 65
           PPPPP        D SS   +SP  I+II ++    LL+S Y  + ++C  +  L     
Sbjct: 35  PPPPPFDVAGTSADDSSGPVFSPLVIAIIGVLASAFLLVSYYTFISKYCGTVSSLRGRVF 94

Query: 66  GRSVVQFDENPNDPSPQMHVH---------------GLEASTIRLLPISQFKKNEEAEST 125
           G S           S   H H               GL+ + I  + + ++++ +    T
Sbjct: 95  GSSSGGAAYGGGAGSGGRHGHGQSRSHESWNVSPPSGLDETLINKITVCKYRRGDGFVHT 154

Query: 126 ASINTECAVCLGEFEEGEMIKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCP 165
               T+C+VCLGEF +GE ++ LP C+H+FH  CID WL++HS+CPLCR +++ +++   
Sbjct: 155 ----TDCSVCLGEFSDGESLRLLPRCSHAFHQQCIDTWLKSHSNCPLCRANITFVTVGLA 213

[Close]

BLAST of CmaCh20G004320 vs. ExPASy Swiss-Prot
Match: P0C041 (Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 PE=3 SV=2)

HSP 1 Score: 89.4 bits (220), Expect = 5.5e-17
Identity = 57/162 (35.19%), Postives = 80/162 (49.38%), Query Frame = 0


Query: 8   PPPLS-------PVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCR--VLHELF--C 67
           PPP+S        V    + SP  I+I  I     LL + Y ++ ++C     +E     
Sbjct: 39  PPPISYEQFLNDGVASNPNLSPLVIAIFGIFATAFLLAAYYTLVSKYCANDTTNEAASES 98

Query: 68  GRSVVQFDENPNDPSPQMHVHGLEASTIRL-----LPISQFKKNEEAESTASINTECAVC 127
           GRS +  D N  +   Q     LE+ST  L       I  FK  +         T+C++C
Sbjct: 99  GRSDIILDVNSPERGDQDDPFALESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSIC 158

Query: 128 LGEFEEGEMIKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHV 154
           LGEF E E ++ LP CNH+FH  CID WL++HS+CPLCR  +
Sbjct: 159 LGEFNEDESLRLLPKCNHTFHVVCIDRWLKSHSNCPLCRAKI 200

[Close]

BLAST of CmaCh20G004320 vs. ExPASy TrEMBL
Match: A0A5A7V3S2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250G00390 PE=3 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 3.1e-87
Identity = 165/212 (77.83%), Postives = 183/212 (86.32%), Query Frame = 0


Query: 1   MPSSPPPPPPLSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHELFCGRSV 60
           MPSSPPPP   SPVPDYSSWSPWSIS I IVC++ LLLSNYR+LK+ CRVLHELF GR  
Sbjct: 1   MPSSPPPP---SPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPA 60

Query: 61  VQFDENPNDPSPQMHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEM 120
           VQF+ENPN+PS Q+H HGLE S IRLLPISQFKK EE+EST S NTECAVCLGEFEEGE+
Sbjct: 61  VQFEENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGEL 120

Query: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPTPDSSSAMLETLGREDVDG 180
           IKNLPNCNHSFHSSCIDAW R+HSSCPLCRL V + S    TPDSSSAMLETLGRE++  
Sbjct: 121 IKNLPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFS----TPDSSSAMLETLGRENILR 180

Query: 181 ERVSYYQTLRAQILQNPELRRDASVQGSDQRN 213
           ERV++YQTLRAQILQ+PE RRD S+QGSDQR+
Sbjct: 181 ERVAHYQTLRAQILQHPEFRRDESIQGSDQRD 205

[Close]

BLAST of CmaCh20G004320 vs. ExPASy TrEMBL
Match: A0A1S3BIX6 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490579 PE=3 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 3.1e-87
Identity = 165/212 (77.83%), Postives = 183/212 (86.32%), Query Frame = 0


Query: 1   MPSSPPPPPPLSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHELFCGRSV 60
           MPSSPPPP   SPVPDYSSWSPWSIS I IVC++ LLLSNYR+LK+ CRVLHELF GR  
Sbjct: 1   MPSSPPPP---SPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPA 60

Query: 61  VQFDENPNDPSPQMHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEM 120
           VQF+ENPN+PS Q+H HGLE S IRLLPISQFKK EE+EST S NTECAVCLGEFEEGE+
Sbjct: 61  VQFEENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGEL 120

Query: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPTPDSSSAMLETLGREDVDG 180
           IKNLPNCNHSFHSSCIDAW R+HSSCPLCRL V + S    TPDSSSAMLETLGRE++  
Sbjct: 121 IKNLPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFS----TPDSSSAMLETLGRENILR 180

Query: 181 ERVSYYQTLRAQILQNPELRRDASVQGSDQRN 213
           ERV++YQTLRAQILQ+PE RRD S+QGSDQR+
Sbjct: 181 ERVAHYQTLRAQILQHPEFRRDESIQGSDQRD 205

[Close]

BLAST of CmaCh20G004320 vs. ExPASy TrEMBL
Match: A0A0A0KCA5 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G303210 PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.5e-86
Identity = 168/214 (78.50%), Postives = 184/214 (85.98%), Query Frame = 0


Query: 1   MPSSPPPPPPLSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHELFCGRSV 60
           MPSSPPPP   SPVPDYSSWSPWSIS I +VCIV LLLSNYR+LK+ CRVLH LF GR  
Sbjct: 1   MPSSPPPP---SPVPDYSSWSPWSISTIVVVCIVFLLLSNYRLLKQLCRVLHGLFTGRPT 60

Query: 61  VQFDENPNDPSPQMHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEM 120
           VQF+ENPN+PS Q+H HGLE S IRLLPISQFKKNEE+EST S NTECAVCLGEFEEGE+
Sbjct: 61  VQFEENPNNPSLQIHGHGLETSMIRLLPISQFKKNEESESTTSFNTECAVCLGEFEEGEL 120

Query: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPTPD-SSSAMLETLGREDVD 180
           IK+LPNCNHSFHS CIDAW RNHSSCPLCRL V + S    TPD SSSAMLETLGRE+V 
Sbjct: 121 IKHLPNCNHSFHSPCIDAWFRNHSSCPLCRLQVLSFS----TPDSSSSAMLETLGRENVL 180

Query: 181 GERVSYYQTLRAQILQNPELRRDASVQGSDQRNR 214
            ERV++YQTLRAQILQ+PE RRDAS+QG+DQRNR
Sbjct: 181 RERVAHYQTLRAQILQHPEFRRDASIQGTDQRNR 207

[Close]

BLAST of CmaCh20G004320 vs. ExPASy TrEMBL
Match: A0A067L6V3 (RING-type E3 ubiquitin transferase OS=Jatropha curcas OX=180498 GN=JCGZ_05653 PE=3 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 9.0e-39
Identity = 98/203 (48.28%), Postives = 126/203 (62.07%), Query Frame = 0


Query: 1   MPSSPPPPPPLSPVPDYSS--WSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHELFCGR 60
           + SSPPP     P  D+ S  W+P+ I  +  +CIVV++ S Y+I+KR C  L+ L   R
Sbjct: 4   LTSSPPPVSEFHP-EDFPSQQWNPYVIGPLIAMCIVVVIFSYYKIIKRLCCALNTLTFSR 63

Query: 61  SVVQFDE-NPNDPSPQMHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEE 120
           + VQ    +  DPS Q   HGLE+  +  LPISQFKK +E E T   N+ECAVCLGEFEE
Sbjct: 64  NHVQTRHLSVEDPSLQYQSHGLESIVMDSLPISQFKKEKEQEETRQSNSECAVCLGEFEE 123

Query: 121 GEMIKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPTPDSSSAMLETLGRED 180
           GE +K+LPNC H FH +CID W + HSSCPLCR  V   SI      S + ++E+L RED
Sbjct: 124 GEWLKHLPNCAHVFHVACIDTWFQTHSSCPLCRSLVYENSI------SINTLIESLQRED 183

Query: 181 VDGERVSYYQTLRAQILQNPELR 201
              +R  YYQ LR+QI +N   R
Sbjct: 184 FFHQRAEYYQILRSQIFRNSATR 199

[Close]

BLAST of CmaCh20G004320 vs. ExPASy TrEMBL
Match: A0A6P6XES3 (RING-type E3 ubiquitin transferase OS=Coffea arabica OX=13443 GN=LOC113742631 PE=3 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 2.0e-38
Identity = 92/197 (46.70%), Postives = 126/197 (63.96%), Query Frame = 0


Query: 3   SSPPPPPPLSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWC----RVLHELFCGR 62
           S PP PPP S   ++S W+P  I+++ +VC + LL S  +IL+R C     + +    G+
Sbjct: 48  SMPPSPPPTSGF-EHSQWNPMVIALVVVVCCIFLLFSYQKILQRHCSHFGSITNSRNQGQ 107

Query: 63  SVVQFDENPNDPSPQMHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEG 122
           S    D   +DPS Q+   GL++  +  LPI+QFKKN E E+   I T+CAVCLGEFEEG
Sbjct: 108 SRRLHDAVADDPSLQLQSRGLDSFVMHSLPITQFKKNNEEETCKGI-TDCAVCLGEFEEG 167

Query: 123 EMIKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPTPDSSSAMLETLGREDV 182
           E +K+LP+C+H FH SCIDAW + HSSCPLCR +V NL+++     S   +LETL RE+ 
Sbjct: 168 EWLKHLPHCSHVFHVSCIDAWFQTHSSCPLCRSYVFNLTMHQEHSVSVYTLLETLRREEF 227

Query: 183 DGERVSYYQTLRAQILQ 196
             ER   YQ LR+ +LQ
Sbjct: 228 SQERAENYQILRSHVLQ 242

[Close]

BLAST of CmaCh20G004320 vs. NCBI nr
Match: XP_023512261.1 (RING-H2 finger protein ATL74-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 419.9 bits (1078), Expect = 1.4e-113
Identity = 205/213 (96.24%), Postives = 209/213 (98.12%), Query Frame = 0


Query: 1   MPSSPPPPPPLSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHELFCGRSV 60
           MPSSPPPPPP SPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRV H LFCGRSV
Sbjct: 1   MPSSPPPPPPPSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVFHGLFCGRSV 60

Query: 61  VQFDENPNDPSPQMHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEM 120
           VQFDENPNDPSPQ+HVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGE+
Sbjct: 61  VQFDENPNDPSPQVHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEL 120

Query: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPTPDSSSAMLETLGREDVDG 180
           IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSI+CPTPDSSSAMLETL REDVDG
Sbjct: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSIDCPTPDSSSAMLETLSREDVDG 180

Query: 181 ERVSYYQTLRAQILQNPELRRDASVQGSDQRNR 214
           ERVSYYQTLRAQILQNP+LRRDASVQGSDQRNR
Sbjct: 181 ERVSYYQTLRAQILQNPDLRRDASVQGSDQRNR 213

[Close]

BLAST of CmaCh20G004320 vs. NCBI nr
Match: KAG6570731.1 (RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010575.1 RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 416.8 bits (1070), Expect = 1.1e-112
Identity = 204/213 (95.77%), Postives = 209/213 (98.12%), Query Frame = 0


Query: 1   MPSSPPPPPPLSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHELFCGRSV 60
           MPSSPPPPPP SPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLH LFCGR V
Sbjct: 1   MPSSPPPPPPPSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHGLFCGRFV 60

Query: 61  VQFDENPNDPSPQMHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEM 120
           VQFDENPNDPSP++HVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGE+
Sbjct: 61  VQFDENPNDPSPEVHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEL 120

Query: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPTPDSSSAMLETLGREDVDG 180
           IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSI+CPTPDSSSAMLETLGREDV+ 
Sbjct: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSIDCPTPDSSSAMLETLGREDVER 180

Query: 181 ERVSYYQTLRAQILQNPELRRDASVQGSDQRNR 214
           ERVSYYQTLRAQILQNPELRRDASVQGSDQRNR
Sbjct: 181 ERVSYYQTLRAQILQNPELRRDASVQGSDQRNR 213

[Close]

BLAST of CmaCh20G004320 vs. NCBI nr
Match: XP_008448367.1 (PREDICTED: RING-H2 finger protein ATL66-like [Cucumis melo] >KAA0061954.1 RING-H2 finger protein ATL66-like [Cucumis melo var. makuwa] >TYK23976.1 RING-H2 finger protein ATL66-like [Cucumis melo var. makuwa])

HSP 1 Score: 331.3 bits (848), Expect = 6.3e-87
Identity = 165/212 (77.83%), Postives = 183/212 (86.32%), Query Frame = 0


Query: 1   MPSSPPPPPPLSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHELFCGRSV 60
           MPSSPPPP   SPVPDYSSWSPWSIS I IVC++ LLLSNYR+LK+ CRVLHELF GR  
Sbjct: 1   MPSSPPPP---SPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPA 60

Query: 61  VQFDENPNDPSPQMHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEM 120
           VQF+ENPN+PS Q+H HGLE S IRLLPISQFKK EE+EST S NTECAVCLGEFEEGE+
Sbjct: 61  VQFEENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGEL 120

Query: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPTPDSSSAMLETLGREDVDG 180
           IKNLPNCNHSFHSSCIDAW R+HSSCPLCRL V + S    TPDSSSAMLETLGRE++  
Sbjct: 121 IKNLPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFS----TPDSSSAMLETLGRENILR 180

Query: 181 ERVSYYQTLRAQILQNPELRRDASVQGSDQRN 213
           ERV++YQTLRAQILQ+PE RRD S+QGSDQR+
Sbjct: 181 ERVAHYQTLRAQILQHPEFRRDESIQGSDQRD 205

[Close]

BLAST of CmaCh20G004320 vs. NCBI nr
Match: XP_031743630.1 (RING-H2 finger protein ATL52-like [Cucumis sativus] >KGN47355.1 hypothetical protein Csa_023057 [Cucumis sativus])

HSP 1 Score: 328.9 bits (842), Expect = 3.1e-86
Identity = 168/214 (78.50%), Postives = 184/214 (85.98%), Query Frame = 0


Query: 1   MPSSPPPPPPLSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHELFCGRSV 60
           MPSSPPPP   SPVPDYSSWSPWSIS I +VCIV LLLSNYR+LK+ CRVLH LF GR  
Sbjct: 1   MPSSPPPP---SPVPDYSSWSPWSISTIVVVCIVFLLLSNYRLLKQLCRVLHGLFTGRPT 60

Query: 61  VQFDENPNDPSPQMHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEM 120
           VQF+ENPN+PS Q+H HGLE S IRLLPISQFKKNEE+EST S NTECAVCLGEFEEGE+
Sbjct: 61  VQFEENPNNPSLQIHGHGLETSMIRLLPISQFKKNEESESTTSFNTECAVCLGEFEEGEL 120

Query: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPTPD-SSSAMLETLGREDVD 180
           IK+LPNCNHSFHS CIDAW RNHSSCPLCRL V + S    TPD SSSAMLETLGRE+V 
Sbjct: 121 IKHLPNCNHSFHSPCIDAWFRNHSSCPLCRLQVLSFS----TPDSSSSAMLETLGRENVL 180

Query: 181 GERVSYYQTLRAQILQNPELRRDASVQGSDQRNR 214
            ERV++YQTLRAQILQ+PE RRDAS+QG+DQRNR
Sbjct: 181 RERVAHYQTLRAQILQHPEFRRDASIQGTDQRNR 207

[Close]

BLAST of CmaCh20G004320 vs. NCBI nr
Match: KAF3453818.1 (hypothetical protein FNV43_RR04259 [Rhamnella rubrinervis])

HSP 1 Score: 171.8 bits (434), Expect = 6.4e-39
Identity = 90/196 (45.92%), Postives = 127/196 (64.80%), Query Frame = 0


Query: 12  SPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHELFCGRSVVQFDENPNDPS 71
           SP+P  S W+PW+I+ +A V I++LLLS +RIL+ +C          S++  + N +DPS
Sbjct: 305 SPLPVPSHWNPWAIASVATVSIIILLLSYFRILQSFCCNFSSRNQAGSLLLSETNLDDPS 364

Query: 72  PQMHVHGLEASTIRLLPISQFK-KNEEAESTASINTECAVCLGEFEEGEMIKNLPNCNHS 131
            Q H HGLE S I  LPI+QFK +N  ++     +T+CAVCLGEF+EGE ++ LPNC H+
Sbjct: 365 LQFHSHGLEVSLIHSLPITQFKEENGGSKMGHQTSTDCAVCLGEFQEGEWLRFLPNCKHA 424

Query: 132 FHSSCIDAWLRNHSSCPLCRLHVSNLSI-NCPTPDSSSAMLETLGREDVDGERVSYYQTL 191
           FH SCID W  +HS+CPLCR  V+  +  +   P S   +LETL RED   ER ++YQ L
Sbjct: 425 FHISCIDTWFLSHSTCPLCRSEVNGFAFEDHEYPVSMYTLLETLRREDFFQERAAHYQFL 484

Query: 192 RAQILQNPELRRDASV 206
           ++ IL N   R ++S+
Sbjct: 485 QSTILNNSVARTESSL 500

[Close]

BLAST of CmaCh20G004320 vs. TAIR 10
Match: AT5G43420.1 (RING/U-box superfamily protein )

HSP 1 Score: 101.7 bits (252), Expect = 7.6e-22
Identity = 68/185 (36.76%), Postives = 99/185 (53.51%), Query Frame = 0


Query: 5   PPPPPPL--SPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHEL-FCGR-SV 64
           PPPPPP+        +S+   ++++I I+    LL+S Y  + + C   H +   GR S+
Sbjct: 17  PPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSL 76

Query: 65  VQFDENPNDP----SPQMHVHGLEASTIRLLPISQFKK----------NEEAESTASINT 124
            +   N  DP    SP++   GL+ S IR +PI +FKK           E  E     + 
Sbjct: 77  SRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQ 136

Query: 125 ECAVCLGEFEEGEMIKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPT---P 169
           EC+VCL EF++ E ++ +PNC+H FH  CID WL+N+++CPLCR  VS     C T   P
Sbjct: 137 ECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVS-----CDTSFPP 196

[Close]

BLAST of CmaCh20G004320 vs. TAIR 10
Match: AT1G04360.1 (RING/U-box superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 9.3e-20
Identity = 63/164 (38.41%), Postives = 90/164 (54.88%), Query Frame = 0


Query: 5   PPPPPP----LSPVPDYSSWSPWSISIIAIVCIVV-----LLLSNYRILKRWCRVLHEL- 64
           PPPPPP     SP+   +S S  +  I+AI  I +     LL+S Y  + + C   H++ 
Sbjct: 17  PPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQID 76

Query: 65  FCGRSVVQFDENP-NDPSPQMHVHGLEASTIRLLPISQFKKNEEA---ESTASINTECAV 124
              R     D+NP    SP     GL+ S IR +P+ +FKK +     E  +  + EC+V
Sbjct: 77  IFRRRRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNSQECSV 136

Query: 125 CLGEFEEGEMIKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVS 155
           CL EF+E E ++ +PNC H FH  CID WL+ +++CPLCR  VS
Sbjct: 137 CLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVS 180

[Close]

BLAST of CmaCh20G004320 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 9.3e-20
Identity = 56/166 (33.73%), Postives = 89/166 (53.61%), Query Frame = 0


Query: 6   PPPPPLSPVPD-----YSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHELFCGRSV 65
           PPPPP   + D      SS+SP  I++I I+   ++L+S Y ++ ++C   H+     ++
Sbjct: 35  PPPPPSFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETL 94

Query: 66  VQFDENPNDPSPQMHV----HGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFE 125
                     S    +     GL  S I+ + + ++K  +         ++C+VCL EFE
Sbjct: 95  NLNHNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVD----GSDCSVCLSEFE 154

Query: 126 EGEMIKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCPT 163
           E E ++ LP CNH+FH  CID WL++HS+CPLCR  V+   +N PT
Sbjct: 155 ENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFVT--GVNNPT 194

[Close]

BLAST of CmaCh20G004320 vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 89.4 bits (220), Expect = 3.9e-18
Identity = 57/162 (35.19%), Postives = 80/162 (49.38%), Query Frame = 0


Query: 8   PPPLS-------PVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCR--VLHELF--C 67
           PPP+S        V    + SP  I+I  I     LL + Y ++ ++C     +E     
Sbjct: 39  PPPISYEQFLNDGVASNPNLSPLVIAIFGIFATAFLLAAYYTLVSKYCANDTTNEAASES 98

Query: 68  GRSVVQFDENPNDPSPQMHVHGLEASTIRL-----LPISQFKKNEEAESTASINTECAVC 127
           GRS +  D N  +   Q     LE+ST  L       I  FK  +         T+C++C
Sbjct: 99  GRSDIILDVNSPERGDQDDPFALESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSIC 158

Query: 128 LGEFEEGEMIKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHV 154
           LGEF E E ++ LP CNH+FH  CID WL++HS+CPLCR  +
Sbjct: 159 LGEFNEDESLRLLPKCNHTFHVVCIDRWLKSHSNCPLCRAKI 200

[Close]

BLAST of CmaCh20G004320 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 87.0 bits (214), Expect = 1.9e-17
Identity = 68/197 (34.52%), Postives = 95/197 (48.22%), Query Frame = 0


Query: 5   PPPPPPLSPVPDYSSWSPWSISII-AIVCIVVLLLSNYRILKRWCRV-LHELFCGRSVVQ 64
           PPPPPP  P  + S   P SI ++ +I+  + L+   Y  + +  R+    +  G +  Q
Sbjct: 26  PPPPPPPPPPRELSLLLPTSICVVGSIILFLFLVFFLYLHITQQRRISAASVTPGDTNQQ 85

Query: 65  FDENPNDPSPQMHVH--------GLEASTIRLLPISQFKKNEEAESTASINTECAVCLGE 124
            DE+  +       H        GL  S I  + +  FKK E         TEC+VCL E
Sbjct: 86  EDEDETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIID----GTECSVCLNE 145

Query: 125 FEEGEMIKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSINCP---------TPDS 183
           FEE E ++ LP C+H+FH +CID WL +H +CPLCR  V  L I  P          PDS
Sbjct: 146 FEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPV--LLITEPPHQETETNHQPDS 205

The following BLAST results are available for this feature:

BLAST of CmaCh20G004320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSW9	1.1e-20	36.76	RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1	[more]
P93823	1.3e-18	38.41	RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1	[more]
Q9LF64	1.3e-18	33.73	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]
Q7XLY8	1.1e-17	31.15	E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994...	[more]
P0C041	5.5e-17	35.19	Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 P...	[more]

BLAST of CmaCh20G004320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7V3S2	3.1e-87	77.83	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BIX6	3.1e-87	77.83	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490579 PE=3 ...	[more]
A0A0A0KCA5	1.5e-86	78.50	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G303210 PE...	[more]
A0A067L6V3	9.0e-39	48.28	RING-type E3 ubiquitin transferase OS=Jatropha curcas OX=180498 GN=JCGZ_05653 PE...	[more]
A0A6P6XES3	2.0e-38	46.70	RING-type E3 ubiquitin transferase OS=Coffea arabica OX=13443 GN=LOC113742631 PE...	[more]

BLAST of CmaCh20G004320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023512261.1	1.4e-113	96.24	RING-H2 finger protein ATL74-like [Cucurbita pepo subsp. pepo]	[more]
KAG6570731.1	1.1e-112	95.77	RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]
XP_008448367.1	6.3e-87	77.83	PREDICTED: RING-H2 finger protein ATL66-like [Cucumis melo] >KAA0061954.1 RING-H...	[more]
XP_031743630.1	3.1e-86	78.50	RING-H2 finger protein ATL52-like [Cucumis sativus] >KGN47355.1 hypothetical pro...	[more]
KAF3453818.1	6.4e-39	45.92	hypothetical protein FNV43_RR04259 [Rhamnella rubrinervis]	[more]

BLAST of CmaCh20G004320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43420.1	7.6e-22	36.76	RING/U-box superfamily protein	[more]
AT1G04360.1	9.3e-20	38.41	RING/U-box superfamily protein	[more]
AT5G17600.1	9.3e-20	33.73	RING/U-box superfamily protein	[more]
AT4G17905.1	3.9e-18	35.19	RING/U-box superfamily protein	[more]
AT5G10380.1	1.9e-17	34.52	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 108..149 e-value: 6.8E-7 score: 38.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 107..150 e-value: 2.7E-14 score: 53.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 108..150 score: 12.651561
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 67..155 e-value: 8.2E-22 score: 78.7
IPR044600	RING-H2 finger protein ATL1/ATL16	PANTHER	PTHR46913	RING-H2 FINGER PROTEIN ATL16	coord: 20..164
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 107..150 e-value: 7.61537E-18 score: 71.9493
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 107..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh20G004320.1	CmaCh20G004320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016740	transferase activity