CmaCh20G003190 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G003190
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionGDSL esterase/lipase
LocationCma_Chr20: 1575692 .. 1577779 (+)
RNA-Seq ExpressionCmaCh20G003190
SyntenyCmaCh20G003190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACGAGCTAATTATCTTATTGTTGCTCTATCACTTCACGTAATTTGGCTGTTATTTTTTTTATCAAAACATGCAATGCTTTGGAGGTTAAGATTTCTCGTTATTTTCCTCTAATTCTCATATTCGGTGATTCTACTGTAGATACTGGGAATAACAACTTCATTCCCACTGACTTCAAGGGAAATTACTTTCCATATGATAAAGATTTCTCGAACCATGTTGCCACGGGAAGGTTTAGTAATGGAAAACTCATACCGGACATGATGACCTCTAAATTGGAGATAAAGGAGCTTGTTCTTCCGTTTTTTGATCCTAAATTGTCAAATGATGATATCAAAACAGGGGTCAATTTTGCATCTGCCGGCACAGGCTTCGACGATCTAACATCGACTATGAACAAAGTAATTTCGATGACGAAGCAAATTGATCTTTTCAAGAATTGTACTCAAAGGCTCGAAGGGATTGTAGGTATGGATGAAAGTAAAAGAATTATTAGAGGTGCTTTGGTGGTCGTTAGTGCAGGAACCAATGACCTACTCTTCAATTTCTACGATGATCCTATCAAAAAGTTGAAATACAATATCAGCGGCTATCACGATTTTGTACAAAGCAAACTACAGAGCGTGGTCGAGGTAAATTTTATATAGTATGCACTCATTAATCCTAATCAATATTTGACACAAGGTTAAATTATAAAAAAAAATATCCTGCAATTTAGATCGGTACACCATTTTAGCTAGATCAACATTAAGTCGTTCCAAGTTTCAATTAATTTTTTTGAGTTCTTGAAAATATTCTAATTTACCTATTTTTGTTCGAAAACACTTCGGGCTAATTTTATTTTAGGGAATTTACAATCTTGGATGTCCGAATATTATAGTTGTTGGACTCCCTCCAATCGGGTGTCTTCCCATTCAAGAGACTATCATTTAAAGATCTCCCTCGAAAATGTGTAGTAGATCAAAATTCAAATTCAGAGGCCTATAATCAAAAGCTACTAAAGTTGTAAAAAAATTTAGAGGCTAAACTCTCGAGAAGCACGGTTTTGTATGCTGACATTTTCACCCGAATCATGGACATGGTCAATAATCCTAAAAAATATGGTAAGTCTGAAAAGATTTGAGAGAAATCAAGTTATGAAAAAAAAATCAATTTAATTAATGTGAATTTTGTTTGGGGATTTTATCAAACGGGTTTTGAGCAGACGAACAAAAGTTGTTGTGGAACTGGATTGACTGAAGCAGGGCCTCTATGTAATGCATTAATCCCAACGTGCAAAAACCGATCAAAATTTGTATTTTGGGATAGCTTTCATCCGACTGAAACAGCCTATAACTTCATCACTGAGTCGCTTTTCAATCAGTTTTTCGATCACGTGAATTGAAATTGATGAGTTTCAACCGAGCTCTAGTTTTATGGCATTATGGGTTTAAAAGTATGTCGTAAGTTACAATAATAATTGACAAAAAAAAAAAATTAAGAGAAAATAAGATCTATATATTCACTTTACCTCGGAACAAAAATGGGATGAAAAACAGCCAACTTACCACCGGTAAAAAAAGAAAAAGAAAAAGAACCCGAAATTACAGCTCCGATACGTTTTATCATCTCGGCCGAAAAATCGGATCCCGGCGCCATCGAAAAACAAAACTTTTACATGTGAAGTATATTATTTTAGTCTTCAGAAATGCTCTCTAAACGGGGCCGCCATACGACAACCCGGGCGCCGCGGCGGTCCCGCCGTGACGGTTTCTTGTCGGATACAACATCGGAGAGGTAAGGATCACTGTCTCCGGTGGTGGTGCAAGATGGAGTTTTGAGGCTGCTTCCGGCGTTTCTTTTCTTCTCCGGTAAAGAATTCGCCGGAATCAAGAAATAAATAGTGCCCCTTTTGAGCTCAGATTCCGGCGAGAGGATCAGAATCCGACGAACGACCTCTTGTGAAGAAGGCTTACTAAGAACATGATTTGGGTTCGCTTTTAGGACATCGCCGGCGGTGATGGGATGGGCAAATTCATCGATGTGGCCATTCAAATGAACGATTCGGATCAAATCAAGAGCACCGCAAGGAAGAACACAAGCTAA

mRNA sequence

ATGGCACGAGCTAATTATCTTATTGTTGCTCTATCACTTCACATTTCTCGTTATTTTCCTCTAATTCTCATATTCGGTGATTCTACTGTAGATACTGGGAATAACAACTTCATTCCCACTGACTTCAAGGGAAATTACTTTCCATATGATAAAGATTTCTCGAACCATGTTGCCACGGGAAGGTTTAGTAATGGAAAACTCATACCGGACATGATGACCTCTAAATTGGAGATAAAGGAGCTTGTTCTTCCGTTTTTTGATCCTAAATTGTCAAATGATGATATCAAAACAGGGGTCAATTTTGCATCTGCCGGCACAGGCTTCGACGATCTAACATCGACTATGAACAAAGTAATTTCGATGACGAAGCAAATTGATCTTTTCAAGAATTGTACTCAAAGGCTCGAAGGGATTGTAGGTATGGATGAAAGTAAAAGAATTATTAGAGGTGCTTTGGTGGTCGTTAGTGCAGGAACCAATGACCTACTCTTCAATTTCTACGATGATCCTATCAAAAAGTTGAAATACAATATCAGCGGCTATCACGATTTTGTACAAAGCAAACTACAGAGCGTGGTCGAGTTGTTGGACTCCCTCCAATCGGGTGTCTTCCCATTCAAGAGACTATCATTTAAAGATCTCCCTCGAAAATGTGTAGTAGATCAAAATTCAAATTCAGAGGCCTATAATCAAAAGCTACTAAAGTTGCTAAACTCTCGAGAAGCACGGTTTTGTATGCTGACATTTTCACCCGAATCATGGACATGGTCAATAATCCTAAAAAATATGACGAACAAAAGTTGTTGTGGAACTGGATTGACTGAAGCAGGGCCTCTATGTAATGCATTAATCCCAACGTGCAAAAACCGATCAAAATTTGTATTTTGGGATAGCTTTCATCCGACTGAAACAGCCTATAACTTCATCACTGAGTCGCTTTTCAATCAGTTTTTCGATCACAAATGCTCTCTAAACGGGGCCGCCATACGACAACCCGGGCGCCGCGGCGGTCCCGCCGTGACGGACATCGCCGGCGGTGATGGGATGGGCAAATTCATCGATGTGGCCATTCAAATGAACGATTCGGATCAAATCAAGAGCACCGCAAGGAAGAACACAAGCTAA

Coding sequence (CDS)

ATGGCACGAGCTAATTATCTTATTGTTGCTCTATCACTTCACATTTCTCGTTATTTTCCTCTAATTCTCATATTCGGTGATTCTACTGTAGATACTGGGAATAACAACTTCATTCCCACTGACTTCAAGGGAAATTACTTTCCATATGATAAAGATTTCTCGAACCATGTTGCCACGGGAAGGTTTAGTAATGGAAAACTCATACCGGACATGATGACCTCTAAATTGGAGATAAAGGAGCTTGTTCTTCCGTTTTTTGATCCTAAATTGTCAAATGATGATATCAAAACAGGGGTCAATTTTGCATCTGCCGGCACAGGCTTCGACGATCTAACATCGACTATGAACAAAGTAATTTCGATGACGAAGCAAATTGATCTTTTCAAGAATTGTACTCAAAGGCTCGAAGGGATTGTAGGTATGGATGAAAGTAAAAGAATTATTAGAGGTGCTTTGGTGGTCGTTAGTGCAGGAACCAATGACCTACTCTTCAATTTCTACGATGATCCTATCAAAAAGTTGAAATACAATATCAGCGGCTATCACGATTTTGTACAAAGCAAACTACAGAGCGTGGTCGAGTTGTTGGACTCCCTCCAATCGGGTGTCTTCCCATTCAAGAGACTATCATTTAAAGATCTCCCTCGAAAATGTGTAGTAGATCAAAATTCAAATTCAGAGGCCTATAATCAAAAGCTACTAAAGTTGCTAAACTCTCGAGAAGCACGGTTTTGTATGCTGACATTTTCACCCGAATCATGGACATGGTCAATAATCCTAAAAAATATGACGAACAAAAGTTGTTGTGGAACTGGATTGACTGAAGCAGGGCCTCTATGTAATGCATTAATCCCAACGTGCAAAAACCGATCAAAATTTGTATTTTGGGATAGCTTTCATCCGACTGAAACAGCCTATAACTTCATCACTGAGTCGCTTTTCAATCAGTTTTTCGATCACAAATGCTCTCTAAACGGGGCCGCCATACGACAACCCGGGCGCCGCGGCGGTCCCGCCGTGACGGACATCGCCGGCGGTGATGGGATGGGCAAATTCATCGATGTGGCCATTCAAATGAACGATTCGGATCAAATCAAGAGCACCGCAAGGAAGAACACAAGCTAA

Protein sequence

MARANYLIVALSLHISRYFPLILIFGDSTVDTGNNNFIPTDFKGNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFASAGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLLFNFYDDPIKKLKYNISGYHDFVQSKLQSVVELLDSLQSGVFPFKRLSFKDLPRKCVVDQNSNSEAYNQKLLKLLNSREARFCMLTFSPESWTWSIILKNMTNKSCCGTGLTEAGPLCNALIPTCKNRSKFVFWDSFHPTETAYNFITESLFNQFFDHKCSLNGAAIRQPGRRGGPAVTDIAGGDGMGKFIDVAIQMNDSDQIKSTARKNTS
Homology
BLAST of CmaCh20G003190 vs. ExPASy Swiss-Prot
Match: Q9SIQ2 (GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3 SV=3)

HSP 1 Score: 250.0 bits (637), Expect = 4.3e-65
Identity = 142/323 (43.96%), Postives = 189/323 (58.51%), Query Frame = 0

Query: 19  FPLILIFGDSTVDTGNNNF-IPTDFKGNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLE 78
           FP ILIFGDSTVDTGNNN+ +PT F+  +FPY  D  +  A GRFSNGKLI D++ +KL 
Sbjct: 33  FPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKLISDIIATKLN 92

Query: 79  IKELVLPFFDPKLSNDDIKTGVNFASAGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEG 138
           IKE + PF  P LS+ DI TGV FASAG G+DDLTS   + I +++Q ++FK+   RL+G
Sbjct: 93  IKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNMFKSYIARLKG 152

Query: 139 IVGMDESKRIIRGALVVVSAGTNDLLFNFYDDPIKKLKYN-ISGYHDFVQSKLQSVVELL 198
           IVG  ++  II  A VVVSAG ND + N+YD P ++L+Y  ISGY DF+  +L++ V  L
Sbjct: 153 IVGDKKAMEIINNAFVVVSAGPNDFILNYYDIPSRRLEYPFISGYQDFILKRLENFVREL 212

Query: 199 DSL-----------QSGVFPFKRLS-FKDLPRKCVVDQNSNSEAYNQKLLKLLNSREARF 258
            SL             G  P    + F+++ R C+   N +S  YN+KL KLL   EA  
Sbjct: 213 YSLGVRNVLVGGLPPMGCLPIHMTAKFRNIFRFCLEHHNKDSVLYNEKLQKLLPQIEASL 272

Query: 259 CMLTF--------------SPESWTWSIILKNMTNKSCCGTGLTEAGPLCNALIPTCKNR 314
               F              +P  + +       T + CCGTG  E   +CN   P C+NR
Sbjct: 273 PGSKFLYADVYNPMMEMIQNPSKYGF-----KETKRGCCGTGFLETSFMCNVFSPVCQNR 332

BLAST of CmaCh20G003190 vs. ExPASy Swiss-Prot
Match: O22927 (GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.6e-64
Identity = 143/329 (43.47%), Postives = 187/329 (56.84%), Query Frame = 0

Query: 9   VALSLHISRYFPLILIFGDSTVDTGNNNF-IPTDFKGNYFPYDKDFSNHVATGRFSNGKL 68
           VA +      FP ILIFGDSTVDTGNNN+   T FK  + PY  D   H A GR+SNGK+
Sbjct: 22  VAANATTQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKV 81

Query: 69  IPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFASAGTGFDDLTSTMNKVISMTKQIDL 128
           I D++ SKL IKELV PF  P +S+ DI TGV+FASAG G+DD +S  +K I +++Q  +
Sbjct: 82  ISDVIASKLNIKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSM 141

Query: 129 FKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLLFNFYDDPIKKLKY-NISGYHDFVQ 188
           FKN   RL+GIVG  ++  II  ALVV+SAG ND + NFYD P ++L+Y  I GY +F+ 
Sbjct: 142 FKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFIL 201

Query: 189 SKLQSVVELLDSL-----------QSGVFPFKRLS-FKDLPRKCVVDQNSNSEAYNQKLL 248
            +L   V  L SL             G  P +  +  +++ R CV  +N +S  YNQKL+
Sbjct: 202 KRLDGFVRELYSLGCRNIVVGGLPPMGCLPIQMTAKMRNILRFCVEQENKDSVLYNQKLV 261

Query: 249 KLLNSREARFCMLTF--------------SPESWTWSIILKNMTNKSCCGTGLTEAGPLC 308
           K L   +A      F              +P  + +       T K CCGTG  E   +C
Sbjct: 262 KKLPEIQASLPGSNFLYANVYDPLMDMIQNPSKYGF-----KETKKGCCGTGYLETTFMC 321

Query: 309 NALIPTCKNRSKFVFWDSFHPTETAYNFI 310
           N L  TC N S  +FWDS HP+E AYN+I
Sbjct: 322 NPLTKTCPNHSDHLFWDSIHPSEAAYNYI 345

BLAST of CmaCh20G003190 vs. ExPASy Swiss-Prot
Match: Q9SIQ3 (GDSL esterase/lipase At2g31540 OS=Arabidopsis thaliana OX=3702 GN=At2g31540 PE=2 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 8.1e-64
Identity = 139/319 (43.57%), Postives = 187/319 (58.62%), Query Frame = 0

Query: 19  FPLILIFGDSTVDTGNNNF-IPTDFKGNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLE 78
           FP ILIFGDSTVDTGNNN+ +PT F+  +FPY  D  +  A GRFSNGKLI D++ +KL 
Sbjct: 33  FPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKLISDIIATKLN 92

Query: 79  IKELVLPFFDPKLSNDDIKTGVNFASAGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEG 138
           IKE + PF  P LS+ DI TGV FASAG G+DDLTS   + I +++Q ++FK+   RL+G
Sbjct: 93  IKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNMFKSYIARLKG 152

Query: 139 IVGMDESKRIIRGALVVVSAGTNDLLFNFYDDPIKKLKYN-ISGYHDFVQSKLQSVVELL 198
           IVG  ++  II  A VVVSAG ND + N+Y+ P ++L+Y  ISGY DF+  +L++ V  L
Sbjct: 153 IVGDKKAMEIINNAFVVVSAGPNDFILNYYEIPSRRLEYPFISGYQDFILKRLENFVREL 212

Query: 199 DSL-----------QSGVFPFKRLS-FKDLPRKCVVDQNSNSEAYNQKLLKLLNSREARF 258
            SL             G  P    + F+++ R C+   N +S  YN+KL  LL   EA  
Sbjct: 213 YSLGVRNVLVGGLPPMGCLPIHMTAKFRNIFRFCLEHHNKDSVLYNEKLQNLLPQIEASL 272

Query: 259 CMLTF--------------SPESWTWSIILKNMTNKSCCGTGLTEAGPLCNALIPTCKNR 310
               F              +P  + +       T + CCGTG  E   +CN   P C+NR
Sbjct: 273 PGSKFLYADVYNPMMEMIQNPSKYGF-----KETKRGCCGTGFLETSFMCNVFSPVCQNR 332

BLAST of CmaCh20G003190 vs. ExPASy Swiss-Prot
Match: Q9SJA9 (GDSL esterase/lipase At2g24560 OS=Arabidopsis thaliana OX=3702 GN=At2g24560 PE=2 SV=2)

HSP 1 Score: 243.0 bits (619), Expect = 5.2e-63
Identity = 139/321 (43.30%), Postives = 189/321 (58.88%), Query Frame = 0

Query: 19  FPLILIFGDSTVDTGNNNF-IPTDFKGNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLE 78
           FP ILIFGDSTVDTGNNN+   T FK  + PY  D  NH A+GRF+NGK+  D++ +KL 
Sbjct: 32  FPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGIDLPNHKASGRFTNGKIFSDIIATKLN 91

Query: 79  IKELVLPFFDPKLSNDDIKTGVNFASAGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEG 138
           IK+ V PF  P LS+ +I TGV FASAG G+DD TS   + I +  Q  +FKN   RL+ 
Sbjct: 92  IKQFVPPFLQPNLSDQEIVTGVCFASAGAGYDDHTSLSTQAIRVLDQQKMFKNYIARLKS 151

Query: 139 IVGMDESKRIIRGALVVVSAGTNDLLFNFYDDPIKKLKY-NISGYHDFVQSKLQSVVELL 198
           IVG  ++  II+ ALVV+SAG ND + N+YD P ++L++ +ISGY DFV  +L + V  L
Sbjct: 152 IVGDKKAMEIIKNALVVISAGPNDFILNYYDIPSRRLEFPHISGYQDFVLQRLDNFVREL 211

Query: 199 DSL-----------QSGVFPFKRLS-FKDLPRKCVVDQNSNSEAYNQKLLKLLNSREARF 258
            SL             G  P +  + F++  R C+  +N +S  YNQKL  LL   EA  
Sbjct: 212 YSLGCRKIMVGGLPPMGCLPIQMTAKFRNALRFCLEQENRDSVLYNQKLQNLLPQIEASL 271

Query: 259 --CMLTFSPESWTWSIILKN-------MTNKSCCGTGLTEAGPLCNALIPTCKNRSKFVF 317
               + +S        +++N        T + CCGTG  E   +CNA  PTC+N S+F+F
Sbjct: 272 TGSKILYSNVYDPMMDMMQNPSKYGFKETKRGCCGTGHLETSFMCNAFSPTCRNHSEFLF 331

BLAST of CmaCh20G003190 vs. ExPASy Swiss-Prot
Match: O22918 (GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 8.9e-63
Identity = 141/319 (44.20%), Postives = 179/319 (56.11%), Query Frame = 0

Query: 19  FPLILIFGDSTVDTGNNNFI-PTDFKGNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLE 78
           FP ILIFGDST DTGNNN+     FK N+ PY  D   H A GRFSNGKLI D++++KL 
Sbjct: 31  FPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNGKLISDVISTKLN 90

Query: 79  IKELVLPFFDPKLSNDDIKTGVNFASAGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEG 138
           IKE V PF  P +S+ DI TGV FASAG G+DD TS  +K I +++Q  +FKN   RL+G
Sbjct: 91  IKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQPSMFKNYIARLKG 150

Query: 139 IVGMDESKRIIRGALVVVSAGTNDLLFNFYDDPIKKLKY-NISGYHDFVQSKLQSVVELL 198
           IVG  ++  II  ALVV+SAG ND + NFYD PI++L+Y  I GY DFV  +L   V  L
Sbjct: 151 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDFVLKRLDGFVREL 210

Query: 199 DSL-----------QSGVFPFK-RLSFKDLPRKCVVDQNSNSEAYNQKLLKLLNSREARF 258
            SL             G  P +     + +   CV  +N +S  YNQKL+K L   +A  
Sbjct: 211 YSLGCRNILVGGLPPMGCLPIQLTAKLRTILGICVEQENKDSILYNQKLVKKLPEIQASL 270

Query: 259 CMLTF--------------SPESWTWSIILKNMTNKSCCGTGLTEAGPLCNALIPTCKNR 310
               F              +P  + +       T K CCGTG  E   LC +L  TC N 
Sbjct: 271 PGSKFLYANVYDPVMDMIRNPSKYGF-----KETKKGCCGTGYLETSFLCTSLSKTCPNH 330

BLAST of CmaCh20G003190 vs. ExPASy TrEMBL
Match: A0A6J1FW23 (GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC111448648 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.6e-131
Identity = 262/359 (72.98%), Postives = 274/359 (76.32%), Query Frame = 0

Query: 1   MARANYLIVALSLH-----------------ISRYFPLILIFGDSTVDTGNNNFIPTDFK 60
           MARANYLI ALSLH                 ISR FP ILIFGDSTVDTGNNNFIPT FK
Sbjct: 1   MARANYLIFALSLHVIWLLFFSKLCNASNVKISRSFPAILIFGDSTVDTGNNNFIPTVFK 60

Query: 61  GNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFAS 120
           GNYFPY KDF NHVATGRFSNGKLIPDM  SKL IKELV PF DPKLSNDDIKTGVNFAS
Sbjct: 61  GNYFPYGKDFPNHVATGRFSNGKLIPDMTASKLGIKELVPPFLDPKLSNDDIKTGVNFAS 120

Query: 121 AGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLL 180
           AGTGFDDLTSTMNKVISMTKQIDLFKN TQR EGIVGMDESKRII  ALVVVSAGTND+L
Sbjct: 121 AGTGFDDLTSTMNKVISMTKQIDLFKNYTQRFEGIVGMDESKRIISRALVVVSAGTNDVL 180

Query: 181 FNFYDDPIKKLKYNISGYHDFVQSKLQSVVELLDSL-----------QSGVFPFKR-LSF 240
           FNFY+DPIKKLKYNISGYHDF+QSKLQSVVE +  L             G  P +  LSF
Sbjct: 181 FNFYNDPIKKLKYNISGYHDFLQSKLQSVVEEIYHLGCSNIMVAELPPMGCLPIQETLSF 240

Query: 241 KDLP-RKCVVDQNSNSEAYNQKLLKLLNSREARFCMLT-FSPESWTWSIILKN------- 300
           KDLP RKCV DQNS+SEAYNQKLLKLL + EA+    T    + +T  + + N       
Sbjct: 241 KDLPTRKCVEDQNSDSEAYNQKLLKLLKNLEAKLSGSTVLYVDIFTPIMDMVNNPHKYGF 300

Query: 301 -MTNKSCCGTGLTEAGPLCNALIPTCKNRSKFVFWDSFHPTETAYNFITESLFNQFFDH 321
             TNK CCG GL EAGPLCNAL PTCKNRSKFVFWDSFHPTETAYNFITESLFNQ F H
Sbjct: 301 EQTNKGCCGNGLAEAGPLCNALTPTCKNRSKFVFWDSFHPTETAYNFITESLFNQIFGH 359

BLAST of CmaCh20G003190 vs. ExPASy TrEMBL
Match: A0A6J1KWV5 (GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499482 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 5.0e-101
Identity = 204/357 (57.14%), Postives = 248/357 (69.47%), Query Frame = 0

Query: 1   MARANYLIVALSLH-----------------ISRYFPLILIFGDSTVDTGNNNFIPTDFK 60
           MARANY I ALSLH                 ISR FP ILIFGDSTVDTGNNNFIPT FK
Sbjct: 1   MARANYFIAALSLHIIWSLCVTKLCRASDLKISRTFPAILIFGDSTVDTGNNNFIPTIFK 60

Query: 61  GNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFAS 120
            NY PY KDF  H+ATGRFSNGKLIPDM+ S+L IKE V PF DPKL NDD+KTGV+FAS
Sbjct: 61  ANYAPYGKDFPGHIATGRFSNGKLIPDMVASRLGIKEFVPPFLDPKLLNDDVKTGVSFAS 120

Query: 121 AGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLL 180
           AGTGFDDLT+T++KVI + KQIDLFKN  QRL+ IVG+DESK+II  ALV++SAGTNDL 
Sbjct: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYIQRLQRIVGVDESKKIIGSALVIISAGTNDLN 180

Query: 181 FNFYDDPIKKLKYNISGYHDFVQSKLQSVVELLDSL-----------QSGVFPFKR-LSF 240
            NFYD P +KL+YNISGY DF+ ++LQS ++ +  L             G  P +  +SF
Sbjct: 181 INFYDLPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 KD-LPRKCVVDQNSNSEAYNQKLLKLLNSREARF--CMLTFSPESWTWSIILKN------ 300
           ++ L R C+ DQNS+S++YNQKL KLL++ + +     + ++        ++ N      
Sbjct: 241 QNPLNRICLEDQNSDSKSYNQKLSKLLSNLQPQLPRSKMLYADIYTPLMDMINNPHQYGF 300

Query: 301 -MTNKSCCGTGLTEAGPLCNALIPTCKNRSKFVFWDSFHPTETAYNFITESLFNQFF 319
             TN  CCGTGL EAGPLCNAL PTC++ SK++FWDS HPTE+ Y  ITESL  QFF
Sbjct: 301 EHTNTGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLIKQFF 357

BLAST of CmaCh20G003190 vs. ExPASy TrEMBL
Match: A0A6J1G7Z0 (GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC111451580 PE=3 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 6.6e-101
Identity = 206/357 (57.70%), Postives = 251/357 (70.31%), Query Frame = 0

Query: 1   MARANYLIVALSLH-----------------ISRYFPLILIFGDSTVDTGNNNFIPTDFK 60
           MAR NYLI ALSL+                 ISR FP ILIFGDSTVDTGNNNFI T FK
Sbjct: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60

Query: 61  GNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFAS 120
            NY PY KDF  H+ATGRFSNGKLIPDM+ S+L IKELV PF DPKLS DD+KTGV+FAS
Sbjct: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120

Query: 121 AGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLL 180
           AGTGFDDLT+T++KVI + KQIDLFKN TQRL+ I G+DESK+II  ALV++SAGTNDL 
Sbjct: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180

Query: 181 FNFYDDPIKKLKYNISGYHDFVQSKLQSVVELLDSL-----------QSGVFPFKR-LSF 240
            NFYD P +KL+YNISGY DF+ ++LQS ++ +  L             G  P +  +SF
Sbjct: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240

Query: 241 KD-LPRKCVVDQNSNSEAYNQKLLKLLNSREARFCMLT-FSPESWTWSIILKN------- 300
           ++ L R C+ DQNS+S++YNQKL KLL++ +++    T    + +T  I + N       
Sbjct: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300

Query: 301 -MTNKSCCGTGLTEAGPLCNALIPTCKNRSKFVFWDSFHPTETAYNFITESLFNQFF 319
             TN+ CCGTGL EAGPLCNAL PTC++ SK++FWDS HPTE+ Y  ITESL  QFF
Sbjct: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF 357

BLAST of CmaCh20G003190 vs. ExPASy TrEMBL
Match: A0A0A0KF79 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G180980 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 1.2e-99
Identity = 198/358 (55.31%), Postives = 244/358 (68.16%), Query Frame = 0

Query: 1   MARANYLIVALSLHI-----------------SRYFPLILIFGDSTVDTGNNNFIPTDFK 60
           MARANYLI ALS+HI                 SR F  +LIFGDSTVDTGNNNFIPT FK
Sbjct: 1   MARANYLIAALSIHIIWLLFLSKPCSALAPKTSRSFSSVLIFGDSTVDTGNNNFIPTIFK 60

Query: 61  GNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFAS 120
            NY+PY KDF  HVATGRFS+GKLIPDM+ SKL IKELV PF DP+LS+DD+KTGV+FAS
Sbjct: 61  ANYWPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120

Query: 121 AGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLL 180
           AGTG DDLT+ ++KVI   KQID+FKN  QRL+ IVG+DESKRII  AL V+S GTNDL 
Sbjct: 121 AGTGVDDLTAAISKVIPAMKQIDMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180

Query: 181 FNFYDDPIKKLKYNISGYHDFVQSKLQSVVELLDSL-----------QSGVFPFKRLSFK 240
           FNFYD P ++L+YNISGY +F+Q++LQS+++ +  L             G  P +     
Sbjct: 181 FNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISS 240

Query: 241 DLP--RKCVVDQNSNSEAYNQKLLKLLNSREARF--CMLTFSPESWTWSIILKN------ 300
            +P  R+C+  QN ++EAYNQKL KLL S + +     + ++        ++ N      
Sbjct: 241 PIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSQILYADIYTPLMDMINNPQKYGF 300

Query: 301 -MTNKSCCGTGLTEAGPLCNALIPTCKNRSKFVFWDSFHPTETAYNFITESLFNQFFD 320
             TN  CCGTGL EAGPLCN + PTC++ SKF+FWDS HP+E  Y F+TESL NQFFD
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNKITPTCEDPSKFMFWDSIHPSEATYKFVTESLLNQFFD 358

BLAST of CmaCh20G003190 vs. ExPASy TrEMBL
Match: A0A6J1CCH7 (GDSL esterase/lipase At2g30310-like OS=Momordica charantia OX=3673 GN=LOC111009962 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 1.2e-99
Identity = 204/359 (56.82%), Postives = 249/359 (69.36%), Query Frame = 0

Query: 1   MARANYLIVALSLHI-----------------SRYFPLILIFGDSTVDTGNNNFIPTDFK 60
           MARANYLIVALSLH+                 S  FP ILIFGDSTVDTGNNNFIPT FK
Sbjct: 1   MARANYLIVALSLHMILQLFLSKPSSALAPTTSPSFPAILIFGDSTVDTGNNNFIPTVFK 60

Query: 61  GNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFAS 120
            NYFPY KDF +HVATGRFSNGKLIPDM+ SKL IK+LV PF DPKLS+D++KTGV+FAS
Sbjct: 61  ANYFPYGKDFPDHVATGRFSNGKLIPDMVASKLGIKDLVPPFLDPKLSDDEVKTGVSFAS 120

Query: 121 AGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLL 180
           AGTGFDDLT+ + KVI + KQIDLFKN  QRL+GIVG++ESKRII  ALVV+SAGTND  
Sbjct: 121 AGTGFDDLTAIVPKVIPVMKQIDLFKNYIQRLQGIVGVEESKRIIGRALVVISAGTNDFN 180

Query: 181 FNFYDDPIKKLKYNISGYHDFVQSKLQSVVELLDSL-----------QSGVFPFKR-LSF 240
           FNFYD P ++L+++I GY DF+Q++LQ++++ +  L             G  P +  ++F
Sbjct: 181 FNFYDLPTRRLQFDIGGYQDFLQNRLQNLIKEIYQLGCRNMVVAGLPPIGCLPIQETIAF 240

Query: 241 KD-LPRKCVVDQNSNSEAYNQKLLKLLNSREARFCMLT-FSPESWTWSIILKN------- 300
           ++ L RKC+  QN +S+AYNQKL KLL + + +    T    + +T  I + N       
Sbjct: 241 ENPLDRKCLDGQNLDSQAYNQKLSKLLGNLQPQLAGSTILYADIYTPLINMINSPRNYGF 300

Query: 301 -MTNKSCCGTGLTEAGPLCNALIPTCKNRSKFVFWDSFHPTETAYNFITESLFNQFFDH 321
             TN  CCGTGL EAGPLCN    TC+N SKF+FWDS HPTET Y FI +SL  Q  DH
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNPKTATCENPSKFLFWDSIHPTETTYKFIADSLLEQLGDH 359

BLAST of CmaCh20G003190 vs. NCBI nr
Match: XP_022944104.1 (GDSL esterase/lipase At2g30310-like [Cucurbita moschata])

HSP 1 Score: 479.2 bits (1232), Expect = 3.3e-131
Identity = 262/359 (72.98%), Postives = 274/359 (76.32%), Query Frame = 0

Query: 1   MARANYLIVALSLH-----------------ISRYFPLILIFGDSTVDTGNNNFIPTDFK 60
           MARANYLI ALSLH                 ISR FP ILIFGDSTVDTGNNNFIPT FK
Sbjct: 1   MARANYLIFALSLHVIWLLFFSKLCNASNVKISRSFPAILIFGDSTVDTGNNNFIPTVFK 60

Query: 61  GNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFAS 120
           GNYFPY KDF NHVATGRFSNGKLIPDM  SKL IKELV PF DPKLSNDDIKTGVNFAS
Sbjct: 61  GNYFPYGKDFPNHVATGRFSNGKLIPDMTASKLGIKELVPPFLDPKLSNDDIKTGVNFAS 120

Query: 121 AGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLL 180
           AGTGFDDLTSTMNKVISMTKQIDLFKN TQR EGIVGMDESKRII  ALVVVSAGTND+L
Sbjct: 121 AGTGFDDLTSTMNKVISMTKQIDLFKNYTQRFEGIVGMDESKRIISRALVVVSAGTNDVL 180

Query: 181 FNFYDDPIKKLKYNISGYHDFVQSKLQSVVELLDSL-----------QSGVFPFKR-LSF 240
           FNFY+DPIKKLKYNISGYHDF+QSKLQSVVE +  L             G  P +  LSF
Sbjct: 181 FNFYNDPIKKLKYNISGYHDFLQSKLQSVVEEIYHLGCSNIMVAELPPMGCLPIQETLSF 240

Query: 241 KDLP-RKCVVDQNSNSEAYNQKLLKLLNSREARFCMLT-FSPESWTWSIILKN------- 300
           KDLP RKCV DQNS+SEAYNQKLLKLL + EA+    T    + +T  + + N       
Sbjct: 241 KDLPTRKCVEDQNSDSEAYNQKLLKLLKNLEAKLSGSTVLYVDIFTPIMDMVNNPHKYGF 300

Query: 301 -MTNKSCCGTGLTEAGPLCNALIPTCKNRSKFVFWDSFHPTETAYNFITESLFNQFFDH 321
             TNK CCG GL EAGPLCNAL PTCKNRSKFVFWDSFHPTETAYNFITESLFNQ F H
Sbjct: 301 EQTNKGCCGNGLAEAGPLCNALTPTCKNRSKFVFWDSFHPTETAYNFITESLFNQIFGH 359

BLAST of CmaCh20G003190 vs. NCBI nr
Match: XP_023533087.1 (GDSL esterase/lipase At2g30310-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 379.0 bits (972), Expect = 4.6e-101
Identity = 208/357 (58.26%), Postives = 251/357 (70.31%), Query Frame = 0

Query: 1   MARANYLIVALSLH-----------------ISRYFPLILIFGDSTVDTGNNNFIPTDFK 60
           MAR NYLI ALSLH                 ISR FP ILIFGDSTVDTGNNNFI T FK
Sbjct: 1   MARVNYLIGALSLHIILSLCISKLCSASDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60

Query: 61  GNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFAS 120
            NY PY KDF  H+ATGRFSNGKLIPDM+ S+L IKELV PF DPKLSNDD+KTGV+FAS
Sbjct: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVSFAS 120

Query: 121 AGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLL 180
           AGTGFDDLT+T++KVI + KQIDLFKN T+RL+ IVG+DESK+II  ALV++SAGTNDL 
Sbjct: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTKRLQRIVGVDESKKIIGSALVIISAGTNDLN 180

Query: 181 FNFYDDPIKKLKYNISGYHDFVQSKLQSVVELLDSL-----------QSGVFPFKR-LSF 240
            NFYD P +KL+YNISGY DF+ ++LQS ++ +  L             G  P +  +SF
Sbjct: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240

Query: 241 KD-LPRKCVVDQNSNSEAYNQKLLKLLNSREARFCMLT-FSPESWTWSIILKN------- 300
           ++ L R C+ DQNS+S++YNQKL KLL++ + +    T    + +T  I + N       
Sbjct: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQPQLPGSTMLYADIYTPLIDMINNPRQYGF 300

Query: 301 -MTNKSCCGTGLTEAGPLCNALIPTCKNRSKFVFWDSFHPTETAYNFITESLFNQFF 319
             TN  CCGTGL EAGPLCNAL PTC++ SK++FWDS HPTE+ Y  ITESL  QFF
Sbjct: 301 EHTNTGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF 357

BLAST of CmaCh20G003190 vs. NCBI nr
Match: XP_023006812.1 (GDSL esterase/lipase At2g30310-like [Cucurbita maxima])

HSP 1 Score: 377.9 bits (969), Expect = 1.0e-100
Identity = 204/357 (57.14%), Postives = 248/357 (69.47%), Query Frame = 0

Query: 1   MARANYLIVALSLH-----------------ISRYFPLILIFGDSTVDTGNNNFIPTDFK 60
           MARANY I ALSLH                 ISR FP ILIFGDSTVDTGNNNFIPT FK
Sbjct: 1   MARANYFIAALSLHIIWSLCVTKLCRASDLKISRTFPAILIFGDSTVDTGNNNFIPTIFK 60

Query: 61  GNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFAS 120
            NY PY KDF  H+ATGRFSNGKLIPDM+ S+L IKE V PF DPKL NDD+KTGV+FAS
Sbjct: 61  ANYAPYGKDFPGHIATGRFSNGKLIPDMVASRLGIKEFVPPFLDPKLLNDDVKTGVSFAS 120

Query: 121 AGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLL 180
           AGTGFDDLT+T++KVI + KQIDLFKN  QRL+ IVG+DESK+II  ALV++SAGTNDL 
Sbjct: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYIQRLQRIVGVDESKKIIGSALVIISAGTNDLN 180

Query: 181 FNFYDDPIKKLKYNISGYHDFVQSKLQSVVELLDSL-----------QSGVFPFKR-LSF 240
            NFYD P +KL+YNISGY DF+ ++LQS ++ +  L             G  P +  +SF
Sbjct: 181 INFYDLPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 KD-LPRKCVVDQNSNSEAYNQKLLKLLNSREARF--CMLTFSPESWTWSIILKN------ 300
           ++ L R C+ DQNS+S++YNQKL KLL++ + +     + ++        ++ N      
Sbjct: 241 QNPLNRICLEDQNSDSKSYNQKLSKLLSNLQPQLPRSKMLYADIYTPLMDMINNPHQYGF 300

Query: 301 -MTNKSCCGTGLTEAGPLCNALIPTCKNRSKFVFWDSFHPTETAYNFITESLFNQFF 319
             TN  CCGTGL EAGPLCNAL PTC++ SK++FWDS HPTE+ Y  ITESL  QFF
Sbjct: 301 EHTNTGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLIKQFF 357

BLAST of CmaCh20G003190 vs. NCBI nr
Match: XP_022947820.1 (GDSL esterase/lipase At2g30310-like [Cucurbita moschata])

HSP 1 Score: 377.5 bits (968), Expect = 1.4e-100
Identity = 206/357 (57.70%), Postives = 251/357 (70.31%), Query Frame = 0

Query: 1   MARANYLIVALSLH-----------------ISRYFPLILIFGDSTVDTGNNNFIPTDFK 60
           MAR NYLI ALSL+                 ISR FP ILIFGDSTVDTGNNNFI T FK
Sbjct: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60

Query: 61  GNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFAS 120
            NY PY KDF  H+ATGRFSNGKLIPDM+ S+L IKELV PF DPKLS DD+KTGV+FAS
Sbjct: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120

Query: 121 AGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLL 180
           AGTGFDDLT+T++KVI + KQIDLFKN TQRL+ I G+DESK+II  ALV++SAGTNDL 
Sbjct: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180

Query: 181 FNFYDDPIKKLKYNISGYHDFVQSKLQSVVELLDSL-----------QSGVFPFKR-LSF 240
            NFYD P +KL+YNISGY DF+ ++LQS ++ +  L             G  P +  +SF
Sbjct: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240

Query: 241 KD-LPRKCVVDQNSNSEAYNQKLLKLLNSREARFCMLT-FSPESWTWSIILKN------- 300
           ++ L R C+ DQNS+S++YNQKL KLL++ +++    T    + +T  I + N       
Sbjct: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300

Query: 301 -MTNKSCCGTGLTEAGPLCNALIPTCKNRSKFVFWDSFHPTETAYNFITESLFNQFF 319
             TN+ CCGTGL EAGPLCNAL PTC++ SK++FWDS HPTE+ Y  ITESL  QFF
Sbjct: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF 357

BLAST of CmaCh20G003190 vs. NCBI nr
Match: XP_022138897.1 (GDSL esterase/lipase At2g30310-like [Momordica charantia])

HSP 1 Score: 373.2 bits (957), Expect = 2.6e-99
Identity = 204/359 (56.82%), Postives = 249/359 (69.36%), Query Frame = 0

Query: 1   MARANYLIVALSLHI-----------------SRYFPLILIFGDSTVDTGNNNFIPTDFK 60
           MARANYLIVALSLH+                 S  FP ILIFGDSTVDTGNNNFIPT FK
Sbjct: 1   MARANYLIVALSLHMILQLFLSKPSSALAPTTSPSFPAILIFGDSTVDTGNNNFIPTVFK 60

Query: 61  GNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFAS 120
            NYFPY KDF +HVATGRFSNGKLIPDM+ SKL IK+LV PF DPKLS+D++KTGV+FAS
Sbjct: 61  ANYFPYGKDFPDHVATGRFSNGKLIPDMVASKLGIKDLVPPFLDPKLSDDEVKTGVSFAS 120

Query: 121 AGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLL 180
           AGTGFDDLT+ + KVI + KQIDLFKN  QRL+GIVG++ESKRII  ALVV+SAGTND  
Sbjct: 121 AGTGFDDLTAIVPKVIPVMKQIDLFKNYIQRLQGIVGVEESKRIIGRALVVISAGTNDFN 180

Query: 181 FNFYDDPIKKLKYNISGYHDFVQSKLQSVVELLDSL-----------QSGVFPFKR-LSF 240
           FNFYD P ++L+++I GY DF+Q++LQ++++ +  L             G  P +  ++F
Sbjct: 181 FNFYDLPTRRLQFDIGGYQDFLQNRLQNLIKEIYQLGCRNMVVAGLPPIGCLPIQETIAF 240

Query: 241 KD-LPRKCVVDQNSNSEAYNQKLLKLLNSREARFCMLT-FSPESWTWSIILKN------- 300
           ++ L RKC+  QN +S+AYNQKL KLL + + +    T    + +T  I + N       
Sbjct: 241 ENPLDRKCLDGQNLDSQAYNQKLSKLLGNLQPQLAGSTILYADIYTPLINMINSPRNYGF 300

Query: 301 -MTNKSCCGTGLTEAGPLCNALIPTCKNRSKFVFWDSFHPTETAYNFITESLFNQFFDH 321
             TN  CCGTGL EAGPLCN    TC+N SKF+FWDS HPTET Y FI +SL  Q  DH
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNPKTATCENPSKFLFWDSIHPTETTYKFIADSLLEQLGDH 359

BLAST of CmaCh20G003190 vs. TAIR 10
Match: AT2G30310.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 248.1 bits (632), Expect = 1.2e-65
Identity = 143/329 (43.47%), Postives = 187/329 (56.84%), Query Frame = 0

Query: 9   VALSLHISRYFPLILIFGDSTVDTGNNNF-IPTDFKGNYFPYDKDFSNHVATGRFSNGKL 68
           VA +      FP ILIFGDSTVDTGNNN+   T FK  + PY  D   H A GR+SNGK+
Sbjct: 22  VAANATTQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKV 81

Query: 69  IPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFASAGTGFDDLTSTMNKVISMTKQIDL 128
           I D++ SKL IKELV PF  P +S+ DI TGV+FASAG G+DD +S  +K I +++Q  +
Sbjct: 82  ISDVIASKLNIKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSM 141

Query: 129 FKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLLFNFYDDPIKKLKY-NISGYHDFVQ 188
           FKN   RL+GIVG  ++  II  ALVV+SAG ND + NFYD P ++L+Y  I GY +F+ 
Sbjct: 142 FKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFIL 201

Query: 189 SKLQSVVELLDSL-----------QSGVFPFKRLS-FKDLPRKCVVDQNSNSEAYNQKLL 248
            +L   V  L SL             G  P +  +  +++ R CV  +N +S  YNQKL+
Sbjct: 202 KRLDGFVRELYSLGCRNIVVGGLPPMGCLPIQMTAKMRNILRFCVEQENKDSVLYNQKLV 261

Query: 249 KLLNSREARFCMLTF--------------SPESWTWSIILKNMTNKSCCGTGLTEAGPLC 308
           K L   +A      F              +P  + +       T K CCGTG  E   +C
Sbjct: 262 KKLPEIQASLPGSNFLYANVYDPLMDMIQNPSKYGF-----KETKKGCCGTGYLETTFMC 321

Query: 309 NALIPTCKNRSKFVFWDSFHPTETAYNFI 310
           N L  TC N S  +FWDS HP+E AYN+I
Sbjct: 322 NPLTKTCPNHSDHLFWDSIHPSEAAYNYI 345

BLAST of CmaCh20G003190 vs. TAIR 10
Match: AT2G31540.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 245.7 bits (626), Expect = 5.7e-65
Identity = 139/319 (43.57%), Postives = 187/319 (58.62%), Query Frame = 0

Query: 19  FPLILIFGDSTVDTGNNNF-IPTDFKGNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLE 78
           FP ILIFGDSTVDTGNNN+ +PT F+  +FPY  D  +  A GRFSNGKLI D++ +KL 
Sbjct: 33  FPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKLISDIIATKLN 92

Query: 79  IKELVLPFFDPKLSNDDIKTGVNFASAGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEG 138
           IKE + PF  P LS+ DI TGV FASAG G+DDLTS   + I +++Q ++FK+   RL+G
Sbjct: 93  IKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNMFKSYIARLKG 152

Query: 139 IVGMDESKRIIRGALVVVSAGTNDLLFNFYDDPIKKLKYN-ISGYHDFVQSKLQSVVELL 198
           IVG  ++  II  A VVVSAG ND + N+Y+ P ++L+Y  ISGY DF+  +L++ V  L
Sbjct: 153 IVGDKKAMEIINNAFVVVSAGPNDFILNYYEIPSRRLEYPFISGYQDFILKRLENFVREL 212

Query: 199 DSL-----------QSGVFPFKRLS-FKDLPRKCVVDQNSNSEAYNQKLLKLLNSREARF 258
            SL             G  P    + F+++ R C+   N +S  YN+KL  LL   EA  
Sbjct: 213 YSLGVRNVLVGGLPPMGCLPIHMTAKFRNIFRFCLEHHNKDSVLYNEKLQNLLPQIEASL 272

Query: 259 CMLTF--------------SPESWTWSIILKNMTNKSCCGTGLTEAGPLCNALIPTCKNR 310
               F              +P  + +       T + CCGTG  E   +CN   P C+NR
Sbjct: 273 PGSKFLYADVYNPMMEMIQNPSKYGF-----KETKRGCCGTGFLETSFMCNVFSPVCQNR 332

BLAST of CmaCh20G003190 vs. TAIR 10
Match: AT2G24560.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 243.0 bits (619), Expect = 3.7e-64
Identity = 139/321 (43.30%), Postives = 189/321 (58.88%), Query Frame = 0

Query: 19  FPLILIFGDSTVDTGNNNF-IPTDFKGNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLE 78
           FP ILIFGDSTVDTGNNN+   T FK  + PY  D  NH A+GRF+NGK+  D++ +KL 
Sbjct: 32  FPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGIDLPNHKASGRFTNGKIFSDIIATKLN 91

Query: 79  IKELVLPFFDPKLSNDDIKTGVNFASAGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEG 138
           IK+ V PF  P LS+ +I TGV FASAG G+DD TS   + I +  Q  +FKN   RL+ 
Sbjct: 92  IKQFVPPFLQPNLSDQEIVTGVCFASAGAGYDDHTSLSTQAIRVLDQQKMFKNYIARLKS 151

Query: 139 IVGMDESKRIIRGALVVVSAGTNDLLFNFYDDPIKKLKY-NISGYHDFVQSKLQSVVELL 198
           IVG  ++  II+ ALVV+SAG ND + N+YD P ++L++ +ISGY DFV  +L + V  L
Sbjct: 152 IVGDKKAMEIIKNALVVISAGPNDFILNYYDIPSRRLEFPHISGYQDFVLQRLDNFVREL 211

Query: 199 DSL-----------QSGVFPFKRLS-FKDLPRKCVVDQNSNSEAYNQKLLKLLNSREARF 258
            SL             G  P +  + F++  R C+  +N +S  YNQKL  LL   EA  
Sbjct: 212 YSLGCRKIMVGGLPPMGCLPIQMTAKFRNALRFCLEQENRDSVLYNQKLQNLLPQIEASL 271

Query: 259 --CMLTFSPESWTWSIILKN-------MTNKSCCGTGLTEAGPLCNALIPTCKNRSKFVF 317
               + +S        +++N        T + CCGTG  E   +CNA  PTC+N S+F+F
Sbjct: 272 TGSKILYSNVYDPMMDMMQNPSKYGFKETKRGCCGTGHLETSFMCNAFSPTCRNHSEFLF 331

BLAST of CmaCh20G003190 vs. TAIR 10
Match: AT2G30220.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 242.3 bits (617), Expect = 6.3e-64
Identity = 141/319 (44.20%), Postives = 179/319 (56.11%), Query Frame = 0

Query: 19  FPLILIFGDSTVDTGNNNFI-PTDFKGNYFPYDKDFSNHVATGRFSNGKLIPDMMTSKLE 78
           FP ILIFGDST DTGNNN+     FK N+ PY  D   H A GRFSNGKLI D++++KL 
Sbjct: 31  FPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNGKLISDVISTKLN 90

Query: 79  IKELVLPFFDPKLSNDDIKTGVNFASAGTGFDDLTSTMNKVISMTKQIDLFKNCTQRLEG 138
           IKE V PF  P +S+ DI TGV FASAG G+DD TS  +K I +++Q  +FKN   RL+G
Sbjct: 91  IKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQPSMFKNYIARLKG 150

Query: 139 IVGMDESKRIIRGALVVVSAGTNDLLFNFYDDPIKKLKY-NISGYHDFVQSKLQSVVELL 198
           IVG  ++  II  ALVV+SAG ND + NFYD PI++L+Y  I GY DFV  +L   V  L
Sbjct: 151 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDFVLKRLDGFVREL 210

Query: 199 DSL-----------QSGVFPFK-RLSFKDLPRKCVVDQNSNSEAYNQKLLKLLNSREARF 258
            SL             G  P +     + +   CV  +N +S  YNQKL+K L   +A  
Sbjct: 211 YSLGCRNILVGGLPPMGCLPIQLTAKLRTILGICVEQENKDSILYNQKLVKKLPEIQASL 270

Query: 259 CMLTF--------------SPESWTWSIILKNMTNKSCCGTGLTEAGPLCNALIPTCKNR 310
               F              +P  + +       T K CCGTG  E   LC +L  TC N 
Sbjct: 271 PGSKFLYANVYDPVMDMIRNPSKYGF-----KETKKGCCGTGYLETSFLCTSLSKTCPNH 330

BLAST of CmaCh20G003190 vs. TAIR 10
Match: AT1G06990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 240.7 bits (613), Expect = 1.8e-63
Identity = 139/341 (40.76%), Postives = 195/341 (57.18%), Query Frame = 0

Query: 6   YLIVALSLHISRYFPLILIFGDSTVDTGNNNFIPTDFKGNYFPYDKDFSNHVATGRFSNG 65
           Y+I   ++++S  FP IL+FGDST+DTGNNN+I T  + N+ PY  +F  H ATGRFSNG
Sbjct: 23  YVINVTNVNVS-MFPAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNG 82

Query: 66  KLIPDMMTSKLEIKELVLPFFDPKLSNDDIKTGVNFASAGTGFDDLTSTMNKVISMTKQI 125
           KLIPD + S + IK+ V PF DP LS+ DI TGV FASAG+G+D+LT      +S+ KQ 
Sbjct: 83  KLIPDFIASLMGIKDTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQA 142

Query: 126 DLFKNCTQRLEGIVGMDESKRIIRGALVVVSAGTNDLLFNFYDDPIKKLKYNISGYHDFV 185
           D+ ++  +RL  IVG +++  I+  ALV+VS+GTND   N YD P ++ K  + GY  F+
Sbjct: 143 DMLRSYVERLSQIVGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFI 202

Query: 186 QSKLQSVVELLDSL-----------QSGVFPFKRLSF--KDLPRKCVVDQNSNSEAYNQK 245
            S + + V+ L  +             G  P +      K   R+C+  QNS+S+ +NQK
Sbjct: 203 LSNVHNFVQELYDIGCRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQK 262

Query: 246 LLKLLNSRE---------------ARFCMLTFSPESWTWSIILKNMTNKSCCGTGLTEAG 305
           L   L   +               A F M T +P+ +     LK  T + CCGTG  E  
Sbjct: 263 LKNSLTEMQSNLTGSVIFYGDIYGALFDMAT-NPQRYG----LKE-TTRGCCGTGEIELA 322

Query: 306 PLCNALIPTCKNRSKFVFWDSFHPTETAYNFITESLFNQFF 319
            LCNAL   C N ++++FWD  HP++ AY  I+ SL  Q F
Sbjct: 323 YLCNALTRICPNPNQYLFWDDIHPSQIAYIVISLSLVEQIF 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SIQ24.3e-6543.96GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3... [more]
O229271.6e-6443.47GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2... [more]
Q9SIQ38.1e-6443.57GDSL esterase/lipase At2g31540 OS=Arabidopsis thaliana OX=3702 GN=At2g31540 PE=2... [more]
Q9SJA95.2e-6343.30GDSL esterase/lipase At2g24560 OS=Arabidopsis thaliana OX=3702 GN=At2g24560 PE=2... [more]
O229188.9e-6344.20GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3... [more]
Match NameE-valueIdentityDescription
A0A6J1FW231.6e-13172.98GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC11144864... [more]
A0A6J1KWV55.0e-10157.14GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499482 ... [more]
A0A6J1G7Z06.6e-10157.70GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC11145158... [more]
A0A0A0KF791.2e-9955.31Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G180980 PE=3 SV=1[more]
A0A6J1CCH71.2e-9956.82GDSL esterase/lipase At2g30310-like OS=Momordica charantia OX=3673 GN=LOC1110099... [more]
Match NameE-valueIdentityDescription
XP_022944104.13.3e-13172.98GDSL esterase/lipase At2g30310-like [Cucurbita moschata][more]
XP_023533087.14.6e-10158.26GDSL esterase/lipase At2g30310-like [Cucurbita pepo subsp. pepo][more]
XP_023006812.11.0e-10057.14GDSL esterase/lipase At2g30310-like [Cucurbita maxima][more]
XP_022947820.11.4e-10057.70GDSL esterase/lipase At2g30310-like [Cucurbita moschata][more]
XP_022138897.12.6e-9956.82GDSL esterase/lipase At2g30310-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G30310.11.2e-6543.47GDSL-like Lipase/Acylhydrolase family protein [more]
AT2G31540.15.7e-6543.57GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT2G24560.13.7e-6443.30GDSL-like Lipase/Acylhydrolase family protein [more]
AT2G30220.16.3e-6444.20GDSL-like Lipase/Acylhydrolase family protein [more]
AT1G06990.11.8e-6340.76GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 12..318
e-value: 7.0E-45
score: 155.8
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 22..312
e-value: 1.9E-21
score: 77.6
NoneNo IPR availablePANTHERPTHR45642:SF30GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 17..312
NoneNo IPR availablePANTHERPTHR45642GDSL ESTERASE/LIPASE EXL3coord: 17..312
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 21..320

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G003190.1CmaCh20G003190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds