CmaCh20G001150 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G001150
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionCalcium dependent protein kinase
LocationCma_Chr20: 536731 .. 540175 (+)
RNA-Seq ExpressionCmaCh20G001150
SyntenyCmaCh20G001150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGGTCAGATATTACCATATACAATAAAGGACTCCATTTTAGATTACGGAATTGATGATATATTGTCGTTATACATATCATATACCATCAAACGTGATTGGTTATGCATTTAAGACATTGTCGTATATGATTAAAGATTATGTTTCGTGTCATGGAGTAGATGACGTATTGCCATGATAATTGCGATCTCGATGATCCGTAAAAACAATAAATATATGAAACGTAGGAACTGATATATTAAATCTTGATTATTATTGATGAGTGTATCAACCTGTTTTATGTTATAAACTAATCTGGTACAAATTACCATATAATTGCATAAATGAAATGCCTGTATAAATATATATAATATATATGAAAGTTTTATTTTTATTTTTATTTTGGTATGTTGTTATTTTGTTTCATTTGAACTTCAAAAGTAAATACGTGTTGGTTTTTTTTAATTTAATTTAAAGTAGGAAACAAACATTTTTTATTTCAATTTATAGTATGCATTAGTGTCGTATTAAATAAAGCATTTAAAAAGCCTTATTAAGGGGAAAAAAGCTGAATAATAAGGAAATCAATATTGTTAAAAAAAAAATGATGTAAAAAGAACTGCCCGGCCGGAGCGGAATTGCATCCGTGGGAGTCAGCTACGGCGGCGATAAAGCATCCGTGAACTTAGACTCGGCGGAAATTAGAAGTGTAAATGCAGTGAAAAATTAATTGGGCCTTTTGCACTTTGATGGGCTTTCAATTGGGCCTTCTTCACATACAAATTTGGGTTAGCTCCAATTGGGCCCTAAAAAAGAAAAAAGAAAAAGGAAAAGAAAAGGATATTACCATATTTCGGATCTAAAAGATATTCTAGAAAAAGGAATTCCAAAAAAAGAAAGAAAGAAAAAAAAAAGAAAAAACAATAAAGAAATGGAAGAAAAATATTAAAGGAAAAAAACAGTTAAAATGAAGCGCGAAAGGCTGAGAACCTTAGAACTGGGGAGGGATTAAGGAAGACCGTGTCTATGGCTTCCTCCTACAATTCCTCTCTATCAATCCTCAGCGTTAGGATCAGAATTCCAAGCTGTCTTCTCCTCAATTTCCACCATTTTTCTTCCTTCTTTTTCTCTTTCTTTCCCGTTCTCTATACATGGATTTTTCTCCCTTGCTCACTTTTGGTTCTCCCCCAAATACCCATTCGATCCTGTCTTCTTCAAGTGCCCTACCCTTACCGCCGCCGCCATCCTCCCTCCCCATTCCTCCGCTATGGGCAATTGTTGCTCTCGTGAAAATCCCGACGAGCCTCCCACCAGCGTGAAGGATGGATCTAACGCGGACAAGTCCAAGGATGATGGCGGAAATTCTAATCAGGGCAACTCCACCGAGAATCCATCGAATAAAGCCGCTTCTTCGGCCTCGCCGGCGGATGCCGCTAAGAATACAAAGCCCTCTCAGATCGGAACTGTTTTAGGGCGGCCGATGGAAGATGTTCGATCCATTTATTCCATTGGTAAGGAGCTGGGAAGGGGTCAATTCGGGGTAACCCATTTATGTACGCACAAGACTACTGGCGAACAGTTGGCGTGCAAAACCATTGCTAAGAGGAAGCTCGTAAATAAGGAGGATATTGAGGATGTTAGAAGGGAGGTCCAGATTATGCACCATTTAACTGGTCAGCCCAACACTGTTGAGCTTAAAGGAGCTTTTGAAGATAAGCATTCTGTTCATTTAGTCATGGAGTTGTGCGCTGGAGGAGAGTTGTTTGATCGCATCATAGCGAAAGGGCATTACACAGAGCGAGCTGCGGCTTCGTTGCTCAGGACTATTGTGCAGATTGTTCATACTTGTCATTCCATGGGGGTTATTCATAGAGATCTCAAGCCTGAAAACTTCCTTTTGCTGAATAAGGAAGAGGATTCTCCGCTCAAAGCTACCGATTTCGGTTTATCCGTATTCTATAAGCAAGGTTTGCTCCATAACCTGACTGTTTCTGTTATAAATCTCGGCTGATTATGTTGTTCTTATTCTGGTATGATTGTTGCTATTATATAGGAGAGGTATTTAAGGATATTGTTGGTAGTGCATATTATATTGCACCAGAAGTCTTGAAACGGAAATATGGACCTGAAGTTGATATTTGGAGCGTTGGAGTCATGCTCTACATTTTACTGTGTGGAGTTCCACCTTTCTGGGCAGGTAGTTTATTAGTTTTATGAATCTCTCTGCTGGGTTTGATCTTTTTGATAAGAGAAAATTGAATGGCAGAATCTGAACATGGGATATTCAACGCAATCTTGCGTGGCCATGTCGATTTTACTAGCGATCCATGGCCTACAATATCACCTGCAGCAAAGGATCTTGTAAGGAAGATGTTGAATTCAGACCCCAAGCAGAGATTGACAGCCTCCCAAGTTCTAAGTAAGTTCATCCCCGCATGAAGTTGTATGATAGAATGGTTGAATCTATGAAGGAAAATTAGGAAGAGAAAAGGCAGGTTTATGAATTATGACCATCATTTCCTTCTCTATCAGGCCATCCATGGATTAAGGAGGATGGAGAAGCACCTGATACACCACTTGACAATGCTGTGCTCAATAGGCTCAAACAGTTCCGTGCAATGAACAAATTCAAGAAAGTCGCCCTTCGGGTAAGTGATAATGAACAACAAAGCATTTTGGAATTATATATAATGTGCCAAATGGGCAGGTGATTGCGGGCTGTCTATCAGAGGAAGAAATCATGGGGCTCAAACAGATGTTCAAGGGCATGGATACTGACAACAGCGGGACAATAACGCTCGAAGAGCTAAAGCAAGGACTCGCAAAGCAAGGCACAAAACTCTCTGAATATGAAGTTAAACAATTAATGGAAGCCGTAAGTCATTGCTTAATCCCTATGAATCCGTAATGAGTTTTCTTGCTTGAAGAATCTGGATATTACCTTTTGGTTTCTACAGGCTGATGCTGATGGCAATGGAACCATAGACTATGACGAGTTCATTACAGCGACAATGCACCTGAACAGAATGGATAGGGAAGAGCATCTTTACACTGCCTTCCAGTATTTCGACAAAGATAACAGCGGGTAAACGGAATCGCATTCAAGTCGATTATAGCTAAAGTTTTCATTTTGAAATGGATAGGGAGTGTCACTAATGATTGAACCTCAAACTTCGTTATGCATTCAGATACATCACTACGGAAGAGTTAGAACAAGCTCTTAGAGAATACGGCATGCACGATGGAAGGGACATTAAGGAGATACTTTCTGAAGTGGATGCAGACAATGTAAGTTACTAAAAATCCACATCCTCATACTGCTGCAGGTGGAGAGAGACCAGAAACTGACCTGTGTTTTGAATTTGTACAGGATGGTCGCATCAACTATGATGAATTTGTGGCGATGATGCGAAAAGGAAATCCAGAAGCGAATCCCAAAAAGCGTCGTGATGTTTTCGTTTGA

mRNA sequence

ATGATTGCGTTAGGATCAGAATTCCAAGCTGTCTTCTCCTCAATTTCCACCATTTTTCTTCCTTCTTTTTCTCTTTCTTTCCCGTTCTCTATACATGGATTTTTCTCCCTTGCTCACTTTTGGTTCTCCCCCAAATACCCATTCGATCCTGTCTTCTTCAAGTGCCCTACCCTTACCGCCGCCGCCATCCTCCCTCCCCATTCCTCCGCTATGGGCAATTGTTGCTCTCGTGAAAATCCCGACGAGCCTCCCACCAGCGTGAAGGATGGATCTAACGCGGACAAGTCCAAGGATGATGGCGGAAATTCTAATCAGGGCAACTCCACCGAGAATCCATCGAATAAAGCCGCTTCTTCGGCCTCGCCGGCGGATGCCGCTAAGAATACAAAGCCCTCTCAGATCGGAACTGTTTTAGGGCGGCCGATGGAAGATGTTCGATCCATTTATTCCATTGGTAAGGAGCTGGGAAGGGGTCAATTCGGGGTAACCCATTTATGTACGCACAAGACTACTGGCGAACAGTTGGCGTGCAAAACCATTGCTAAGAGGAAGCTCGTAAATAAGGAGGATATTGAGGATGTTAGAAGGGAGGTCCAGATTATGCACCATTTAACTGGTCAGCCCAACACTGTTGAGCTTAAAGGAGCTTTTGAAGATAAGCATTCTGTTCATTTAGTCATGGAGTTGTGCGCTGGAGGAGAGTTGTTTGATCGCATCATAGCGAAAGGGCATTACACAGAGCGAGCTGCGGCTTCGTTGCTCAGGACTATTGTGCAGATTGTTCATACTTGTCATTCCATGGGGGTTATTCATAGAGATCTCAAGCCTGAAAACTTCCTTTTGCTGAATAAGGAAGAGGATTCTCCGCTCAAAGCTACCGATTTCGGTTTATCCGTATTCTATAAGCAAGGAGAGGTATTTAAGGATATTGTTGGTAGTGCATATTATATTGCACCAGAAGTCTTGAAACGGAAATATGGACCTGAAGTTGATATTTGGAGCGTTGGAGTCATGCTCTACATTTTACTGTGTGGAGTTCCACCTTTCTGGGCAGAATCTGAACATGGGATATTCAACGCAATCTTGCGTGGCCATGTCGATTTTACTAGCGATCCATGGCCTACAATATCACCTGCAGCAAAGGATCTTGTAAGGAAGATGTTGAATTCAGACCCCAAGCAGAGATTGACAGCCTCCCAAGTTCTAAGCCATCCATGGATTAAGGAGGATGGAGAAGCACCTGATACACCACTTGACAATGCTGTGCTCAATAGGCTCAAACAGTTCCGTGCAATGAACAAATTCAAGAAAGTCGCCCTTCGGGTGATTGCGGGCTGTCTATCAGAGGAAGAAATCATGGGGCTCAAACAGATGTTCAAGGGCATGGATACTGACAACAGCGGGACAATAACGCTCGAAGAGCTAAAGCAAGGACTCGCAAAGCAAGGCACAAAACTCTCTGAATATGAAGTTAAACAATTAATGGAAGCCGCTGATGCTGATGGCAATGGAACCATAGACTATGACGAGTTCATTACAGCGACAATGCACCTGAACAGAATGGATAGGGAAGAGCATCTTTACACTGCCTTCCAGTATTTCGACAAAGATAACAGCGGATACATCACTACGGAAGAGTTAGAACAAGCTCTTAGAGAATACGGCATGCACGATGGAAGGGACATTAAGGAGATACTTTCTGAAGTGGATGCAGACAATGATGGTCGCATCAACTATGATGAATTTGTGGCGATGATGCGAAAAGGAAATCCAGAAGCGAATCCCAAAAAGCGTCGTGATGTTTTCGTTTGA

Coding sequence (CDS)

ATGATTGCGTTAGGATCAGAATTCCAAGCTGTCTTCTCCTCAATTTCCACCATTTTTCTTCCTTCTTTTTCTCTTTCTTTCCCGTTCTCTATACATGGATTTTTCTCCCTTGCTCACTTTTGGTTCTCCCCCAAATACCCATTCGATCCTGTCTTCTTCAAGTGCCCTACCCTTACCGCCGCCGCCATCCTCCCTCCCCATTCCTCCGCTATGGGCAATTGTTGCTCTCGTGAAAATCCCGACGAGCCTCCCACCAGCGTGAAGGATGGATCTAACGCGGACAAGTCCAAGGATGATGGCGGAAATTCTAATCAGGGCAACTCCACCGAGAATCCATCGAATAAAGCCGCTTCTTCGGCCTCGCCGGCGGATGCCGCTAAGAATACAAAGCCCTCTCAGATCGGAACTGTTTTAGGGCGGCCGATGGAAGATGTTCGATCCATTTATTCCATTGGTAAGGAGCTGGGAAGGGGTCAATTCGGGGTAACCCATTTATGTACGCACAAGACTACTGGCGAACAGTTGGCGTGCAAAACCATTGCTAAGAGGAAGCTCGTAAATAAGGAGGATATTGAGGATGTTAGAAGGGAGGTCCAGATTATGCACCATTTAACTGGTCAGCCCAACACTGTTGAGCTTAAAGGAGCTTTTGAAGATAAGCATTCTGTTCATTTAGTCATGGAGTTGTGCGCTGGAGGAGAGTTGTTTGATCGCATCATAGCGAAAGGGCATTACACAGAGCGAGCTGCGGCTTCGTTGCTCAGGACTATTGTGCAGATTGTTCATACTTGTCATTCCATGGGGGTTATTCATAGAGATCTCAAGCCTGAAAACTTCCTTTTGCTGAATAAGGAAGAGGATTCTCCGCTCAAAGCTACCGATTTCGGTTTATCCGTATTCTATAAGCAAGGAGAGGTATTTAAGGATATTGTTGGTAGTGCATATTATATTGCACCAGAAGTCTTGAAACGGAAATATGGACCTGAAGTTGATATTTGGAGCGTTGGAGTCATGCTCTACATTTTACTGTGTGGAGTTCCACCTTTCTGGGCAGAATCTGAACATGGGATATTCAACGCAATCTTGCGTGGCCATGTCGATTTTACTAGCGATCCATGGCCTACAATATCACCTGCAGCAAAGGATCTTGTAAGGAAGATGTTGAATTCAGACCCCAAGCAGAGATTGACAGCCTCCCAAGTTCTAAGCCATCCATGGATTAAGGAGGATGGAGAAGCACCTGATACACCACTTGACAATGCTGTGCTCAATAGGCTCAAACAGTTCCGTGCAATGAACAAATTCAAGAAAGTCGCCCTTCGGGTGATTGCGGGCTGTCTATCAGAGGAAGAAATCATGGGGCTCAAACAGATGTTCAAGGGCATGGATACTGACAACAGCGGGACAATAACGCTCGAAGAGCTAAAGCAAGGACTCGCAAAGCAAGGCACAAAACTCTCTGAATATGAAGTTAAACAATTAATGGAAGCCGCTGATGCTGATGGCAATGGAACCATAGACTATGACGAGTTCATTACAGCGACAATGCACCTGAACAGAATGGATAGGGAAGAGCATCTTTACACTGCCTTCCAGTATTTCGACAAAGATAACAGCGGATACATCACTACGGAAGAGTTAGAACAAGCTCTTAGAGAATACGGCATGCACGATGGAAGGGACATTAAGGAGATACTTTCTGAAGTGGATGCAGACAATGATGGTCGCATCAACTATGATGAATTTGTGGCGATGATGCGAAAAGGAAATCCAGAAGCGAATCCCAAAAAGCGTCGTGATGTTTTCGTTTGA

Protein sequence

MIALGSEFQAVFSSISTIFLPSFSLSFPFSIHGFFSLAHFWFSPKYPFDPVFFKCPTLTAAAILPPHSSAMGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNTKPSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKEDIEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAAASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDIVGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTSDPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFRAMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYEVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV
Homology
BLAST of CmaCh20G001150 vs. ExPASy Swiss-Prot
Match: Q9FMP5 (Calcium-dependent protein kinase 17 OS=Arabidopsis thaliana OX=3702 GN=CPK17 PE=2 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 2.2e-252
Identity = 439/536 (81.90%), Postives = 479/536 (89.37%), Query Frame = 0

Query: 71  MGNCCS--RENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAA---SSASPADA 130
           MGNCCS  R++ D          N D  ++    SN  NST  P+ +A+   S  +P   
Sbjct: 1   MGNCCSHGRDSAD----------NGDALENGASASNAANST-GPTAEASVPQSKHAPPSP 60

Query: 131 AKNTKPSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKL 190
              TK   IG VLGRPMEDV++ YS+GKELGRGQFGVTHLCT K TG Q ACKTIAKRKL
Sbjct: 61  PPATKQGPIGPVLGRPMEDVKASYSLGKELGRGQFGVTHLCTQKATGHQFACKTIAKRKL 120

Query: 191 VNKEDIEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHY 250
           VNKEDIEDVRREVQIMHHLTGQPN VELKGA+EDKHSVHLVMELCAGGELFDRIIAKGHY
Sbjct: 121 VNKEDIEDVRREVQIMHHLTGQPNIVELKGAYEDKHSVHLVMELCAGGELFDRIIAKGHY 180

Query: 251 TERAAASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGE 310
           +ERAAASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNK+E+SPLKATDFGLSVFYK GE
Sbjct: 181 SERAAASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKDENSPLKATDFGLSVFYKPGE 240

Query: 311 VFKDIVGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGH 370
           VFKDIVGSAYYIAPEVLKRKYGPE DIWS+GVMLYILLCGVPPFWAESE+GIFNAILRGH
Sbjct: 241 VFKDIVGSAYYIAPEVLKRKYGPEADIWSIGVMLYILLCGVPPFWAESENGIFNAILRGH 300

Query: 371 VDFTSDPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNR 430
           VDF+SDPWP+ISP AKDLV+KMLNSDPKQRLTA+QVL+HPWIKEDGEAPD PLDNAV++R
Sbjct: 301 VDFSSDPWPSISPQAKDLVKKMLNSDPKQRLTAAQVLNHPWIKEDGEAPDVPLDNAVMSR 360

Query: 431 LKQFRAMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTK 490
           LKQF+AMN FKKVALRVIAGCLSEEEIMGLK+MFKGMDTD+SGTITLEEL+QGLAKQGT+
Sbjct: 361 LKQFKAMNNFKKVALRVIAGCLSEEEIMGLKEMFKGMDTDSSGTITLEELRQGLAKQGTR 420

Query: 491 LSEYEVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTE 550
           LSEYEV+QLMEAADADGNGTIDY EFI ATMH+NR+DREEHLY+AFQ+FDKDNSGYIT E
Sbjct: 421 LSEYEVQQLMEAADADGNGTIDYGEFIAATMHINRLDREEHLYSAFQHFDKDNSGYITME 480

Query: 551 ELEQALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDV 602
           ELEQALRE+GM+DGRDIKEI+SEVD DNDGRINYDEFVAMMRKGNP+  PKKRR++
Sbjct: 481 ELEQALREFGMNDGRDIKEIISEVDGDNDGRINYDEFVAMMRKGNPDPIPKKRREL 525

BLAST of CmaCh20G001150 vs. ExPASy Swiss-Prot
Match: Q3E9C0 (Calcium-dependent protein kinase 34 OS=Arabidopsis thaliana OX=3702 GN=CPK34 PE=2 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 5.3e-251
Identity = 433/532 (81.39%), Postives = 473/532 (88.91%), Query Frame = 0

Query: 71  MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNST-ENPSNKAASSASPADAAKNT 130
           MGNCCS             G ++D +K++    N G       ++  AS   PA     T
Sbjct: 1   MGNCCSH------------GRDSDDNKEEPRPENGGGGVGAAEASVRASKHPPASPPPAT 60

Query: 131 KPSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKE 190
           K   IG VLGRPMEDV+S Y++GKELGRGQFGVTHLCT K TG Q ACKTIAKRKLVNKE
Sbjct: 61  KQGPIGPVLGRPMEDVKSSYTLGKELGRGQFGVTHLCTQKATGLQFACKTIAKRKLVNKE 120

Query: 191 DIEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERA 250
           DIEDVRREVQIMHHLTGQPN VELKGA+EDKHSVHLVMELCAGGELFDRIIAKGHY+ERA
Sbjct: 121 DIEDVRREVQIMHHLTGQPNIVELKGAYEDKHSVHLVMELCAGGELFDRIIAKGHYSERA 180

Query: 251 AASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKD 310
           AASLLRTIVQI+HTCHSMGVIHRDLKPENFLLL+K+E+SPLKATDFGLSVFYK GEVFKD
Sbjct: 181 AASLLRTIVQIIHTCHSMGVIHRDLKPENFLLLSKDENSPLKATDFGLSVFYKPGEVFKD 240

Query: 311 IVGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFT 370
           IVGSAYYIAPEVL+RKYGPE DIWS+GVMLYILLCGVPPFWAESE+GIFNAIL G VDF+
Sbjct: 241 IVGSAYYIAPEVLRRKYGPEADIWSIGVMLYILLCGVPPFWAESENGIFNAILSGQVDFS 300

Query: 371 SDPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQF 430
           SDPWP ISP AKDLVRKMLNSDPKQRLTA+QVL+HPWIKEDGEAPD PLDNAV++RLKQF
Sbjct: 301 SDPWPVISPQAKDLVRKMLNSDPKQRLTAAQVLNHPWIKEDGEAPDVPLDNAVMSRLKQF 360

Query: 431 RAMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEY 490
           +AMN FKKVALRVIAGCLSEEEIMGLK+MFKGMDTDNSGTITLEEL+QGLAKQGT+LSEY
Sbjct: 361 KAMNNFKKVALRVIAGCLSEEEIMGLKEMFKGMDTDNSGTITLEELRQGLAKQGTRLSEY 420

Query: 491 EVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQ 550
           EV+QLMEAADADGNGTIDY EFI ATMH+NR+DREEHLY+AFQ+FDKDNSGYITTEELEQ
Sbjct: 421 EVQQLMEAADADGNGTIDYGEFIAATMHINRLDREEHLYSAFQHFDKDNSGYITTEELEQ 480

Query: 551 ALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDV 602
           ALRE+GM+DGRDIKEI+SEVD DNDGRINY+EFVAMMRKGNP+ NPKKRR++
Sbjct: 481 ALREFGMNDGRDIKEIISEVDGDNDGRINYEEFVAMMRKGNPDPNPKKRREL 520

BLAST of CmaCh20G001150 vs. ExPASy Swiss-Prot
Match: Q5VQQ5 (Calcium-dependent protein kinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK2 PE=2 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 4.0e-246
Identity = 424/534 (79.40%), Postives = 469/534 (87.83%), Query Frame = 0

Query: 71  MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADA-AKNT 130
           MGNCC      EP +S                     ST N S+   + ASP+ A A+N 
Sbjct: 1   MGNCCPGSGDAEPASS-------------------DASTGNGSSSFKAGASPSSAPAQNK 60

Query: 131 KPSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKE 190
            P+ IG VLGRPMEDVRSIY+IGKELGRGQFGVT LCTHK TG++ ACKTIAKRKL  KE
Sbjct: 61  PPAPIGPVLGRPMEDVRSIYTIGKELGRGQFGVTSLCTHKATGQKFACKTIAKRKLSTKE 120

Query: 191 DIEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERA 250
           D+EDVRREVQIM+HL GQPN VELKGA+EDK SVHLVMELCAGGELFDRIIAKGHYTERA
Sbjct: 121 DVEDVRREVQIMYHLAGQPNVVELKGAYEDKQSVHLVMELCAGGELFDRIIAKGHYTERA 180

Query: 251 AASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKD 310
           AASLLRTIV+I+HTCHS+GVIHRDLKPENFLLL+K+ED+PLKATDFGLSVF+KQGEVFKD
Sbjct: 181 AASLLRTIVEIIHTCHSLGVIHRDLKPENFLLLSKDEDAPLKATDFGLSVFFKQGEVFKD 240

Query: 311 IVGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFT 370
           IVGSAYYIAPEVLKR YGPE DIWSVGV+LYILLCGVPPFWAESEHGIFN+ILRG VDFT
Sbjct: 241 IVGSAYYIAPEVLKRSYGPEADIWSVGVILYILLCGVPPFWAESEHGIFNSILRGQVDFT 300

Query: 371 SDPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQF 430
           SDPWP IS +AKDLVRKMLNSDPK+R++A +VL+HPWIKEDGEAPDTPLDNAV+NRLKQF
Sbjct: 301 SDPWPRISASAKDLVRKMLNSDPKKRISAYEVLNHPWIKEDGEAPDTPLDNAVMNRLKQF 360

Query: 431 RAMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEY 490
           RAMN+FKK ALRVIAGCLSEEEI GLK+MFK MD+DNSGTIT++EL++GL+KQGTKL+E 
Sbjct: 361 RAMNQFKKAALRVIAGCLSEEEIRGLKEMFKSMDSDNSGTITVDELRKGLSKQGTKLTEA 420

Query: 491 EVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQ 550
           EV+QLMEAADADGNGTIDYDEFITATMH+NRMDREEHLYTAFQYFDKDNSG I+ EELEQ
Sbjct: 421 EVQQLMEAADADGNGTIDYDEFITATMHMNRMDREEHLYTAFQYFDKDNSGCISKEELEQ 480

Query: 551 ALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 604
           ALRE G+ DGRDIK+I+SEVDADNDGRI+Y EF AMMRKGNPEANPKKRRDV +
Sbjct: 481 ALREKGLLDGRDIKDIISEVDADNDGRIDYSEFAAMMRKGNPEANPKKRRDVVI 515

BLAST of CmaCh20G001150 vs. ExPASy Swiss-Prot
Match: B9FKW9 (Calcium-dependent protein kinase 14 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK14 PE=3 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 1.1e-240
Identity = 415/534 (77.72%), Postives = 464/534 (86.89%), Query Frame = 0

Query: 71  MGNCC---SRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAK 130
           MGNCC   S   PD PP S              G+S    S    ++ A  S S A A  
Sbjct: 1   MGNCCPPGSSSEPDPPPAS-------------SGSSRPAGSAGAAASPATISPSAAPAPA 60

Query: 131 NTKPSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVN 190
              P+ IG VLGRPMEDV+SIY++GKELGRGQFGVT LCTHK TG++ ACKTI+KRKL  
Sbjct: 61  K-PPAPIGPVLGRPMEDVKSIYTVGKELGRGQFGVTSLCTHKATGQRFACKTISKRKLST 120

Query: 191 KEDIEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTE 250
           KED+EDVRREVQIM+HL GQP  VELKGA+EDKH+VHLVMELCAGGELFDRIIAKGHYTE
Sbjct: 121 KEDVEDVRREVQIMYHLAGQPGVVELKGAYEDKHAVHLVMELCAGGELFDRIIAKGHYTE 180

Query: 251 RAAASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVF 310
            AA+SLLRTIV+I+HTCHSMGVIHRDLKPENFLLL+K+E +PLKATDFGLSVF+K+GEVF
Sbjct: 181 HAASSLLRTIVEIIHTCHSMGVIHRDLKPENFLLLSKDEHAPLKATDFGLSVFFKEGEVF 240

Query: 311 KDIVGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVD 370
           +DIVGSAYYIAPEVLKR YGPE DIWS+GVMLYILLCGVPPFWAESEHGIFN+ILRGHVD
Sbjct: 241 RDIVGSAYYIAPEVLKRSYGPEADIWSIGVMLYILLCGVPPFWAESEHGIFNSILRGHVD 300

Query: 371 FTSDPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLK 430
           F+S+PW  IS  AKDLVR+ML+SDPKQR++A  VL+HPWIKEDGEAPDTPLDNAVL RLK
Sbjct: 301 FSSEPWSRISHGAKDLVRRMLHSDPKQRISAYDVLNHPWIKEDGEAPDTPLDNAVLGRLK 360

Query: 431 QFRAMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLS 490
           QFRAMN+FKK ALRVIAGCLSEEEI GLK+MFK MD+DNSGTIT++EL++GLAK+GTKL+
Sbjct: 361 QFRAMNQFKKAALRVIAGCLSEEEIRGLKEMFKSMDSDNSGTITVDELRKGLAKKGTKLT 420

Query: 491 EYEVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEEL 550
           E EV+QLMEAADADGNGTIDY+EFITATMH+NRMDREEHLYTAFQYFDKDNSGYIT EEL
Sbjct: 421 EAEVQQLMEAADADGNGTIDYEEFITATMHMNRMDREEHLYTAFQYFDKDNSGYITIEEL 480

Query: 551 EQALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDV 602
           EQALRE G+ DGR+IK+I+SEVDADNDGRINY EFVAMMRKG+PEANPKKRRDV
Sbjct: 481 EQALREKGLMDGREIKDIISEVDADNDGRINYTEFVAMMRKGDPEANPKKRRDV 520

BLAST of CmaCh20G001150 vs. ExPASy Swiss-Prot
Match: Q2RAV0 (Calcium-dependent protein kinase 25 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK25 PE=2 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 9.2e-227
Identity = 389/536 (72.57%), Postives = 457/536 (85.26%), Query Frame = 0

Query: 71  MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTE---NPSNKAASSASPADAAK 130
           MG CC+           + G++       G +S  G++ +   +P+ KAA++ + A A+ 
Sbjct: 1   MGQCCTGGGKAVAGDEAEPGTSKAAPPSRGTSSKNGSAKQQPCSPAAKAAATEAAAAASS 60

Query: 131 NTKPS-QIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLV 190
           + KP+  IG VL RPME+VR+ YSIGKELGRGQFGVTHLCTHK TGE+LACKTIAKRKL 
Sbjct: 61  SKKPAGPIGEVLERPMEEVRTTYSIGKELGRGQFGVTHLCTHKATGEKLACKTIAKRKLA 120

Query: 191 NKEDIEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYT 250
           NKED++DVRREVQIMHHL+GQPN V+L+GA+EDKH+VHLVMELCAGGELFDRIIA+GHYT
Sbjct: 121 NKEDVDDVRREVQIMHHLSGQPNIVDLRGAYEDKHNVHLVMELCAGGELFDRIIARGHYT 180

Query: 251 ERAAASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEV 310
           ERAAA+LLR IV IVHTCHSMGVIHRDLKPENFLLL+K +D+PLKATDFGLSVF+K+GEV
Sbjct: 181 ERAAAALLRAIVGIVHTCHSMGVIHRDLKPENFLLLSKGDDAPLKATDFGLSVFFKEGEV 240

Query: 311 FKDIVGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHV 370
           F+DIVGSAYYIAPEVLKRKYGPE DIWS+GVMLYI L GVPPFWAESE+ IF AILRG +
Sbjct: 241 FRDIVGSAYYIAPEVLKRKYGPEADIWSIGVMLYIFLAGVPPFWAESENAIFTAILRGQI 300

Query: 371 DFTSDPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRL 430
           D  S+PWP IS  AKDLVRKMLN +PK+RLTA QVL+HPWIKEDG+APD PLDN VLNRL
Sbjct: 301 DLASEPWPKISSGAKDLVRKMLNINPKERLTAFQVLNHPWIKEDGDAPDVPLDNVVLNRL 360

Query: 431 KQFRAMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKL 490
           KQFRAMN+FKK ALR+IAGCLSEEEI GLK+MFK +D DNSGTITLEELK GLAKQGTK 
Sbjct: 361 KQFRAMNQFKKAALRIIAGCLSEEEIKGLKEMFKNIDKDNSGTITLEELKNGLAKQGTKF 420

Query: 491 SEYEVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEE 550
           S+ E++QLMEAADADGNG IDY+EF+TAT+H+N+MDREEHLYTAFQYFDKDNSGYIT EE
Sbjct: 421 SDNEIEQLMEAADADGNGIIDYEEFVTATVHMNKMDREEHLYTAFQYFDKDNSGYITKEE 480

Query: 551 LEQALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGN---PEANPKKRR 600
           LEQAL+E G++D  +IK+++++ D++NDGRI+Y EFVAMMRKG+      NPKK+R
Sbjct: 481 LEQALKEQGLYDANEIKDVITDADSNNDGRIDYSEFVAMMRKGSGCAEATNPKKKR 536

BLAST of CmaCh20G001150 vs. ExPASy TrEMBL
Match: A0A6J1JAC9 (calcium-dependent protein kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111483991 PE=4 SV=1)

HSP 1 Score: 1068.5 bits (2762), Expect = 9.9e-309
Identity = 533/533 (100.00%), Postives = 533/533 (100.00%), Query Frame = 0

Query: 71  MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNTK 130
           MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNTK
Sbjct: 1   MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNTK 60

Query: 131 PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED 190
           PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED
Sbjct: 61  PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED 120

Query: 191 IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 250
           IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA
Sbjct: 121 IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 180

Query: 251 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI 310
           ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI
Sbjct: 181 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI 240

Query: 311 VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 370
           VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS
Sbjct: 241 VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 300

Query: 371 DPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR 430
           DPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR
Sbjct: 301 DPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR 360

Query: 431 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE 490
           AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE
Sbjct: 361 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE 420

Query: 491 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 550
           VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA
Sbjct: 421 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 480

Query: 551 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 604
           LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV
Sbjct: 481 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 533

BLAST of CmaCh20G001150 vs. ExPASy TrEMBL
Match: A0A6J1FT91 (calcium-dependent protein kinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111448267 PE=4 SV=1)

HSP 1 Score: 1065.4 bits (2754), Expect = 8.4e-308
Identity = 531/533 (99.62%), Postives = 532/533 (99.81%), Query Frame = 0

Query: 71  MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNTK 130
           MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASP DAAKNTK
Sbjct: 1   MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPGDAAKNTK 60

Query: 131 PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED 190
           PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED
Sbjct: 61  PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED 120

Query: 191 IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 250
           IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA
Sbjct: 121 IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 180

Query: 251 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI 310
           ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI
Sbjct: 181 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI 240

Query: 311 VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 370
           VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS
Sbjct: 241 VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 300

Query: 371 DPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR 430
           DPWP+ISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR
Sbjct: 301 DPWPSISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR 360

Query: 431 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE 490
           AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE
Sbjct: 361 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE 420

Query: 491 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 550
           VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA
Sbjct: 421 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 480

Query: 551 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 604
           LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV
Sbjct: 481 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 533

BLAST of CmaCh20G001150 vs. ExPASy TrEMBL
Match: A0A6J1H160 (calcium-dependent protein kinase 34-like OS=Cucurbita moschata OX=3662 GN=LOC111459487 PE=4 SV=1)

HSP 1 Score: 1010.7 bits (2612), Expect = 2.5e-291
Identity = 516/592 (87.16%), Postives = 536/592 (90.54%), Query Frame = 0

Query: 12  FSSISTIFLPSFSLSFPFSIHGFFSLAHFWFSPKYPFDPVFFKCPTLTAAAILP-PHSSA 71
           FSS    FLP        SIHGFFSL  FWF PKY F+ V F  P+L+     P P SSA
Sbjct: 49  FSSFFFSFLPI------ISIHGFFSLVLFWFCPKYQFNLVSFNRPSLSRRRRHPHPRSSA 108

Query: 72  MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNTK 131
           MGNCCSR+NPD+ PTS ++ SN D SKDDGGN+N        S K A SASP  +AK +K
Sbjct: 109 MGNCCSRDNPDDAPTSTREDSNGDNSKDDGGNTN--------SIKHAPSASPVASAKASK 168

Query: 132 PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED 191
            SQIGTVLGRPMEDVR+ YSIGKELGRGQFGVTHLCTHK +GEQLACKTIAKRKLVNKED
Sbjct: 169 TSQIGTVLGRPMEDVRNTYSIGKELGRGQFGVTHLCTHKASGEQLACKTIAKRKLVNKED 228

Query: 192 IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 251
           IEDVRREVQIMHHLTGQ N VELKGA+EDKHSVHLVMELCAGGELFDRIIAKGHYTERAA
Sbjct: 229 IEDVRREVQIMHHLTGQLNIVELKGAYEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 288

Query: 252 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI 311
           ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLL+K+E SPLKATDFGLSVFYKQGEVFKDI
Sbjct: 289 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLSKDETSPLKATDFGLSVFYKQGEVFKDI 348

Query: 312 VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 371
           VGSAYYIAPEVLKR+YGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS
Sbjct: 349 VGSAYYIAPEVLKRRYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 408

Query: 372 DPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR 431
           DPWP+ISPAAKDLVRKMLNSDPKQRLTA QVL+HPWIKEDGEAPDTPLDNAVLNRLKQFR
Sbjct: 409 DPWPSISPAAKDLVRKMLNSDPKQRLTAFQVLNHPWIKEDGEAPDTPLDNAVLNRLKQFR 468

Query: 432 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE 491
           AMNKFKKVALRVIAGCLSEEEIMGLKQMFK MDTDNSGTITLEELKQGLAKQGTKLSEYE
Sbjct: 469 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKNMDTDNSGTITLEELKQGLAKQGTKLSEYE 528

Query: 492 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 551
           VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA
Sbjct: 529 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 588

Query: 552 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVF 603
           LR+YGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNP+ NPKKRRD F
Sbjct: 589 LRDYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPDGNPKKRRDSF 626

BLAST of CmaCh20G001150 vs. ExPASy TrEMBL
Match: A0A6J1DHQ1 (calcium-dependent protein kinase 2-like OS=Momordica charantia OX=3673 GN=LOC111020598 PE=4 SV=1)

HSP 1 Score: 1005.4 bits (2598), Expect = 1.0e-289
Identity = 500/533 (93.81%), Postives = 515/533 (96.62%), Query Frame = 0

Query: 71  MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNTK 130
           MGNCCSRENPDE P S KD SNAD+SKDDGGN+N GNST NPS   A SASPA + K +K
Sbjct: 1   MGNCCSRENPDEAPNSAKDESNADRSKDDGGNTNHGNST-NPSTNPAPSASPATSTKPSK 60

Query: 131 PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED 190
            +QIGTVLGRPMEDVR++YSIGKELGRGQFGVTHLCTHK TGEQLACKTIAKRKLVNKED
Sbjct: 61  ATQIGTVLGRPMEDVRTVYSIGKELGRGQFGVTHLCTHKATGEQLACKTIAKRKLVNKED 120

Query: 191 IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 250
           IEDVRREVQIMHHLTGQPN VELKGA+EDKHSVHLVMELCAGGELFDRIIAKGHYTERAA
Sbjct: 121 IEDVRREVQIMHHLTGQPNIVELKGAYEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 180

Query: 251 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI 310
           ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLL+K+EDSPLKATDFGLSVFYKQGEVFKDI
Sbjct: 181 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLSKDEDSPLKATDFGLSVFYKQGEVFKDI 240

Query: 311 VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 370
           VGSAYYIAPEVLKR+YGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGH+DFTS
Sbjct: 241 VGSAYYIAPEVLKRRYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHIDFTS 300

Query: 371 DPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR 430
           DPWP+ISPAAKDLVRKMLNSDPKQRL+A QVL+HPWIKEDGEAPDTPLDNAVLNRLKQFR
Sbjct: 301 DPWPSISPAAKDLVRKMLNSDPKQRLSAFQVLNHPWIKEDGEAPDTPLDNAVLNRLKQFR 360

Query: 431 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE 490
           AMNKFKKVALRVIAGCLSEEEIMGLKQMFK MDTDNSGTITLEELKQGLAKQGTKLSEYE
Sbjct: 361 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKSMDTDNSGTITLEELKQGLAKQGTKLSEYE 420

Query: 491 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 550
           VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA
Sbjct: 421 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 480

Query: 551 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 604
           LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV
Sbjct: 481 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 532

BLAST of CmaCh20G001150 vs. ExPASy TrEMBL
Match: A0A1S3BMH2 (calcium-dependent protein kinase 34 OS=Cucumis melo OX=3656 GN=LOC103491467 PE=4 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 1.8e-289
Identity = 503/535 (94.02%), Postives = 517/535 (96.64%), Query Frame = 0

Query: 71  MGNCCSRENPDE-PPTSVKDGS-NADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKN 130
           MGNCCSRENPDE P TSVK+ S NAD SKDDGGN+N GNST NPS+K   SASPA + K 
Sbjct: 1   MGNCCSRENPDEAPATSVKEESNNADNSKDDGGNTNHGNSTNNPSSKTPPSASPAASTKP 60

Query: 131 TKPSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNK 190
           +KPSQIGTVLGRPMEDVRS YSIGKELGRGQFGVTHLCTHK +GEQLACKTIAKRKLVNK
Sbjct: 61  SKPSQIGTVLGRPMEDVRSTYSIGKELGRGQFGVTHLCTHKASGEQLACKTIAKRKLVNK 120

Query: 191 EDIEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTER 250
           EDIEDVRREVQIMHHLTGQPN VELKGA+EDKHSVHLVMELCAGGELFDRIIAKGHYTER
Sbjct: 121 EDIEDVRREVQIMHHLTGQPNIVELKGAYEDKHSVHLVMELCAGGELFDRIIAKGHYTER 180

Query: 251 AAASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFK 310
           AAASLLRTIVQIVHTCHSMGVIHRDLKPENFLLL+K+EDSPLKATDFGLSVFYKQGEVFK
Sbjct: 181 AAASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLSKDEDSPLKATDFGLSVFYKQGEVFK 240

Query: 311 DIVGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDF 370
           DIVGSAYYIAPEVLKR+YGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGH+DF
Sbjct: 241 DIVGSAYYIAPEVLKRRYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHIDF 300

Query: 371 TSDPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQ 430
           TSDPWP+ISPAAKDLVRKMLNSDPKQRL+A QVL+HPWIKEDGEAPDTPLDNAVLNRLKQ
Sbjct: 301 TSDPWPSISPAAKDLVRKMLNSDPKQRLSAFQVLNHPWIKEDGEAPDTPLDNAVLNRLKQ 360

Query: 431 FRAMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSE 490
           FRAMNKFKKVALRVIAGCLSEEEIMGLKQMFK MDTDNSGTITLEELKQGLAKQGTKLSE
Sbjct: 361 FRAMNKFKKVALRVIAGCLSEEEIMGLKQMFKSMDTDNSGTITLEELKQGLAKQGTKLSE 420

Query: 491 YEVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELE 550
           YEVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELE
Sbjct: 421 YEVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELE 480

Query: 551 QALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 604
           QALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV
Sbjct: 481 QALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 535

BLAST of CmaCh20G001150 vs. NCBI nr
Match: XP_022986171.1 (calcium-dependent protein kinase 2-like [Cucurbita maxima] >XP_023512839.1 calcium-dependent protein kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1068.5 bits (2762), Expect = 2.0e-308
Identity = 533/533 (100.00%), Postives = 533/533 (100.00%), Query Frame = 0

Query: 71  MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNTK 130
           MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNTK
Sbjct: 1   MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNTK 60

Query: 131 PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED 190
           PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED
Sbjct: 61  PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED 120

Query: 191 IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 250
           IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA
Sbjct: 121 IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 180

Query: 251 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI 310
           ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI
Sbjct: 181 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI 240

Query: 311 VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 370
           VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS
Sbjct: 241 VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 300

Query: 371 DPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR 430
           DPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR
Sbjct: 301 DPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR 360

Query: 431 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE 490
           AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE
Sbjct: 361 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE 420

Query: 491 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 550
           VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA
Sbjct: 421 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 480

Query: 551 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 604
           LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV
Sbjct: 481 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 533

BLAST of CmaCh20G001150 vs. NCBI nr
Match: XP_022943517.1 (calcium-dependent protein kinase 2-like [Cucurbita moschata])

HSP 1 Score: 1065.4 bits (2754), Expect = 1.7e-307
Identity = 531/533 (99.62%), Postives = 532/533 (99.81%), Query Frame = 0

Query: 71  MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNTK 130
           MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASP DAAKNTK
Sbjct: 1   MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPGDAAKNTK 60

Query: 131 PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED 190
           PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED
Sbjct: 61  PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED 120

Query: 191 IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 250
           IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA
Sbjct: 121 IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 180

Query: 251 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI 310
           ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI
Sbjct: 181 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI 240

Query: 311 VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 370
           VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS
Sbjct: 241 VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 300

Query: 371 DPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR 430
           DPWP+ISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR
Sbjct: 301 DPWPSISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR 360

Query: 431 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE 490
           AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE
Sbjct: 361 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE 420

Query: 491 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 550
           VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA
Sbjct: 421 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 480

Query: 551 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 604
           LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV
Sbjct: 481 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 533

BLAST of CmaCh20G001150 vs. NCBI nr
Match: KAG6570423.1 (Calcium-dependent protein kinase 17, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1063.9 bits (2750), Expect = 5.1e-307
Identity = 530/533 (99.44%), Postives = 531/533 (99.62%), Query Frame = 0

Query: 71  MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNTK 130
           MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNK ASSASP DAAKNTK
Sbjct: 1   MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKGASSASPGDAAKNTK 60

Query: 131 PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED 190
           PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED
Sbjct: 61  PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED 120

Query: 191 IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 250
           IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA
Sbjct: 121 IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 180

Query: 251 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI 310
           ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI
Sbjct: 181 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI 240

Query: 311 VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 370
           VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS
Sbjct: 241 VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 300

Query: 371 DPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR 430
           DPWP+ISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR
Sbjct: 301 DPWPSISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR 360

Query: 431 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE 490
           AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE
Sbjct: 361 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE 420

Query: 491 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 550
           VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA
Sbjct: 421 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 480

Query: 551 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 604
           LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV
Sbjct: 481 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 533

BLAST of CmaCh20G001150 vs. NCBI nr
Match: XP_022958182.1 (calcium-dependent protein kinase 34-like [Cucurbita moschata] >KAG7035889.1 Calcium-dependent protein kinase 17 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1010.7 bits (2612), Expect = 5.1e-291
Identity = 516/592 (87.16%), Postives = 536/592 (90.54%), Query Frame = 0

Query: 12  FSSISTIFLPSFSLSFPFSIHGFFSLAHFWFSPKYPFDPVFFKCPTLTAAAILP-PHSSA 71
           FSS    FLP        SIHGFFSL  FWF PKY F+ V F  P+L+     P P SSA
Sbjct: 49  FSSFFFSFLPI------ISIHGFFSLVLFWFCPKYQFNLVSFNRPSLSRRRRHPHPRSSA 108

Query: 72  MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNTK 131
           MGNCCSR+NPD+ PTS ++ SN D SKDDGGN+N        S K A SASP  +AK +K
Sbjct: 109 MGNCCSRDNPDDAPTSTREDSNGDNSKDDGGNTN--------SIKHAPSASPVASAKASK 168

Query: 132 PSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKED 191
            SQIGTVLGRPMEDVR+ YSIGKELGRGQFGVTHLCTHK +GEQLACKTIAKRKLVNKED
Sbjct: 169 TSQIGTVLGRPMEDVRNTYSIGKELGRGQFGVTHLCTHKASGEQLACKTIAKRKLVNKED 228

Query: 192 IEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 251
           IEDVRREVQIMHHLTGQ N VELKGA+EDKHSVHLVMELCAGGELFDRIIAKGHYTERAA
Sbjct: 229 IEDVRREVQIMHHLTGQLNIVELKGAYEDKHSVHLVMELCAGGELFDRIIAKGHYTERAA 288

Query: 252 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDI 311
           ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLL+K+E SPLKATDFGLSVFYKQGEVFKDI
Sbjct: 289 ASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLSKDETSPLKATDFGLSVFYKQGEVFKDI 348

Query: 312 VGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 371
           VGSAYYIAPEVLKR+YGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS
Sbjct: 349 VGSAYYIAPEVLKRRYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTS 408

Query: 372 DPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFR 431
           DPWP+ISPAAKDLVRKMLNSDPKQRLTA QVL+HPWIKEDGEAPDTPLDNAVLNRLKQFR
Sbjct: 409 DPWPSISPAAKDLVRKMLNSDPKQRLTAFQVLNHPWIKEDGEAPDTPLDNAVLNRLKQFR 468

Query: 432 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYE 491
           AMNKFKKVALRVIAGCLSEEEIMGLKQMFK MDTDNSGTITLEELKQGLAKQGTKLSEYE
Sbjct: 469 AMNKFKKVALRVIAGCLSEEEIMGLKQMFKNMDTDNSGTITLEELKQGLAKQGTKLSEYE 528

Query: 492 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 551
           VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA
Sbjct: 529 VKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQA 588

Query: 552 LREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVF 603
           LR+YGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNP+ NPKKRRD F
Sbjct: 589 LRDYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPDGNPKKRRDSF 626

BLAST of CmaCh20G001150 vs. NCBI nr
Match: XP_038902029.1 (calcium-dependent protein kinase 2-like [Benincasa hispida])

HSP 1 Score: 1005.7 bits (2599), Expect = 1.6e-289
Identity = 503/534 (94.19%), Postives = 514/534 (96.25%), Query Frame = 0

Query: 71  MGNCCSRENPDEPPTSVK-DGSNADKSKDDGGNSNQGNSTENPSNKAASSASPADAAKNT 130
           MGNCCSRENPDE PTSVK + SN D SK DGGN+NQ NS  NPSNK A SASPA + K +
Sbjct: 1   MGNCCSRENPDEAPTSVKGEESNPDNSKGDGGNTNQNNSVNNPSNKPAPSASPAASTKPS 60

Query: 131 KPSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKE 190
           K SQIGTVLGRPMEDVRS YSIGKELGRGQFGVTHLCTHK +GEQLACKTIAKRKLVNKE
Sbjct: 61  KSSQIGTVLGRPMEDVRSTYSIGKELGRGQFGVTHLCTHKASGEQLACKTIAKRKLVNKE 120

Query: 191 DIEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERA 250
           DIEDVRREVQIMHHLTGQPN VELKGA+EDKHSVHLVMELCAGGELFDRIIAKGHYTERA
Sbjct: 121 DIEDVRREVQIMHHLTGQPNIVELKGAYEDKHSVHLVMELCAGGELFDRIIAKGHYTERA 180

Query: 251 AASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKD 310
           AASLLRTIVQIVHTCHSMGVIHRDLKPENFLLL+K+EDSPLKATDFGLSVFYKQGEVFKD
Sbjct: 181 AASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLSKDEDSPLKATDFGLSVFYKQGEVFKD 240

Query: 311 IVGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFT 370
           IVGSAYYIAPEVLKR+YGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGH+DFT
Sbjct: 241 IVGSAYYIAPEVLKRRYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHIDFT 300

Query: 371 SDPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQF 430
           SDPWPTISPAAKDLVRKMLNSDPKQRLTA QVL+HPWIKEDGEAPDTPLDNAVLNRLKQF
Sbjct: 301 SDPWPTISPAAKDLVRKMLNSDPKQRLTAFQVLNHPWIKEDGEAPDTPLDNAVLNRLKQF 360

Query: 431 RAMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEY 490
           RAMNKFKKVALRVIAGCLSEEEIMGLKQMFK MDTDNSGTITLEELKQGLAKQGTKLSEY
Sbjct: 361 RAMNKFKKVALRVIAGCLSEEEIMGLKQMFKSMDTDNSGTITLEELKQGLAKQGTKLSEY 420

Query: 491 EVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQ 550
           EVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQ
Sbjct: 421 EVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQ 480

Query: 551 ALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 604
           ALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV
Sbjct: 481 ALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDVFV 534

BLAST of CmaCh20G001150 vs. TAIR 10
Match: AT5G12180.1 (calcium-dependent protein kinase 17 )

HSP 1 Score: 872.8 bits (2254), Expect = 1.5e-253
Identity = 439/536 (81.90%), Postives = 479/536 (89.37%), Query Frame = 0

Query: 71  MGNCCS--RENPDEPPTSVKDGSNADKSKDDGGNSNQGNSTENPSNKAA---SSASPADA 130
           MGNCCS  R++ D          N D  ++    SN  NST  P+ +A+   S  +P   
Sbjct: 1   MGNCCSHGRDSAD----------NGDALENGASASNAANST-GPTAEASVPQSKHAPPSP 60

Query: 131 AKNTKPSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKL 190
              TK   IG VLGRPMEDV++ YS+GKELGRGQFGVTHLCT K TG Q ACKTIAKRKL
Sbjct: 61  PPATKQGPIGPVLGRPMEDVKASYSLGKELGRGQFGVTHLCTQKATGHQFACKTIAKRKL 120

Query: 191 VNKEDIEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHY 250
           VNKEDIEDVRREVQIMHHLTGQPN VELKGA+EDKHSVHLVMELCAGGELFDRIIAKGHY
Sbjct: 121 VNKEDIEDVRREVQIMHHLTGQPNIVELKGAYEDKHSVHLVMELCAGGELFDRIIAKGHY 180

Query: 251 TERAAASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGE 310
           +ERAAASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNK+E+SPLKATDFGLSVFYK GE
Sbjct: 181 SERAAASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKDENSPLKATDFGLSVFYKPGE 240

Query: 311 VFKDIVGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGH 370
           VFKDIVGSAYYIAPEVLKRKYGPE DIWS+GVMLYILLCGVPPFWAESE+GIFNAILRGH
Sbjct: 241 VFKDIVGSAYYIAPEVLKRKYGPEADIWSIGVMLYILLCGVPPFWAESENGIFNAILRGH 300

Query: 371 VDFTSDPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNR 430
           VDF+SDPWP+ISP AKDLV+KMLNSDPKQRLTA+QVL+HPWIKEDGEAPD PLDNAV++R
Sbjct: 301 VDFSSDPWPSISPQAKDLVKKMLNSDPKQRLTAAQVLNHPWIKEDGEAPDVPLDNAVMSR 360

Query: 431 LKQFRAMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTK 490
           LKQF+AMN FKKVALRVIAGCLSEEEIMGLK+MFKGMDTD+SGTITLEEL+QGLAKQGT+
Sbjct: 361 LKQFKAMNNFKKVALRVIAGCLSEEEIMGLKEMFKGMDTDSSGTITLEELRQGLAKQGTR 420

Query: 491 LSEYEVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTE 550
           LSEYEV+QLMEAADADGNGTIDY EFI ATMH+NR+DREEHLY+AFQ+FDKDNSGYIT E
Sbjct: 421 LSEYEVQQLMEAADADGNGTIDYGEFIAATMHINRLDREEHLYSAFQHFDKDNSGYITME 480

Query: 551 ELEQALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDV 602
           ELEQALRE+GM+DGRDIKEI+SEVD DNDGRINYDEFVAMMRKGNP+  PKKRR++
Sbjct: 481 ELEQALREFGMNDGRDIKEIISEVDGDNDGRINYDEFVAMMRKGNPDPIPKKRREL 525

BLAST of CmaCh20G001150 vs. TAIR 10
Match: AT5G19360.1 (calcium-dependent protein kinase 34 )

HSP 1 Score: 868.2 bits (2242), Expect = 3.8e-252
Identity = 433/532 (81.39%), Postives = 473/532 (88.91%), Query Frame = 0

Query: 71  MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNST-ENPSNKAASSASPADAAKNT 130
           MGNCCS             G ++D +K++    N G       ++  AS   PA     T
Sbjct: 1   MGNCCSH------------GRDSDDNKEEPRPENGGGGVGAAEASVRASKHPPASPPPAT 60

Query: 131 KPSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKE 190
           K   IG VLGRPMEDV+S Y++GKELGRGQFGVTHLCT K TG Q ACKTIAKRKLVNKE
Sbjct: 61  KQGPIGPVLGRPMEDVKSSYTLGKELGRGQFGVTHLCTQKATGLQFACKTIAKRKLVNKE 120

Query: 191 DIEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERA 250
           DIEDVRREVQIMHHLTGQPN VELKGA+EDKHSVHLVMELCAGGELFDRIIAKGHY+ERA
Sbjct: 121 DIEDVRREVQIMHHLTGQPNIVELKGAYEDKHSVHLVMELCAGGELFDRIIAKGHYSERA 180

Query: 251 AASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKD 310
           AASLLRTIVQI+HTCHSMGVIHRDLKPENFLLL+K+E+SPLKATDFGLSVFYK GEVFKD
Sbjct: 181 AASLLRTIVQIIHTCHSMGVIHRDLKPENFLLLSKDENSPLKATDFGLSVFYKPGEVFKD 240

Query: 311 IVGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFT 370
           IVGSAYYIAPEVL+RKYGPE DIWS+GVMLYILLCGVPPFWAESE+GIFNAIL G VDF+
Sbjct: 241 IVGSAYYIAPEVLRRKYGPEADIWSIGVMLYILLCGVPPFWAESENGIFNAILSGQVDFS 300

Query: 371 SDPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQF 430
           SDPWP ISP AKDLVRKMLNSDPKQRLTA+QVL+HPWIKEDGEAPD PLDNAV++RLKQF
Sbjct: 301 SDPWPVISPQAKDLVRKMLNSDPKQRLTAAQVLNHPWIKEDGEAPDVPLDNAVMSRLKQF 360

Query: 431 RAMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEY 490
           +AMN FKKVALRVIAGCLSEEEIMGLK+MFKGMDTDNSGTITLEEL+QGLAKQGT+LSEY
Sbjct: 361 KAMNNFKKVALRVIAGCLSEEEIMGLKEMFKGMDTDNSGTITLEELRQGLAKQGTRLSEY 420

Query: 491 EVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQ 550
           EV+QLMEAADADGNGTIDY EFI ATMH+NR+DREEHLY+AFQ+FDKDNSGYITTEELEQ
Sbjct: 421 EVQQLMEAADADGNGTIDYGEFIAATMHINRLDREEHLYSAFQHFDKDNSGYITTEELEQ 480

Query: 551 ALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRRDV 602
           ALRE+GM+DGRDIKEI+SEVD DNDGRINY+EFVAMMRKGNP+ NPKKRR++
Sbjct: 481 ALREFGMNDGRDIKEIISEVDGDNDGRINYEEFVAMMRKGNPDPNPKKRREL 520

BLAST of CmaCh20G001150 vs. TAIR 10
Match: AT4G23650.1 (calcium-dependent protein kinase 6 )

HSP 1 Score: 701.0 bits (1808), Expect = 8.0e-202
Identity = 346/535 (64.67%), Postives = 430/535 (80.37%), Query Frame = 0

Query: 71  MGNCCSRENPDEPPTSVKDGSNAD------KSKDDGGNSNQGNSTENPSNKAASSASPAD 130
           MG+  S+    +PP S    S+ +       + +  G+S  G    + S    S ++   
Sbjct: 1   MGHRHSKSKSSDPPPSSSSSSSGNVVHHVKPAGERRGSSGSGTVGSSGSGTGGSRST--- 60

Query: 131 AAKNTKPSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRK 190
               T   Q G +LGRPME+VR  Y  G+ELGRGQFGVT+L THK T +Q+ACK+I  R+
Sbjct: 61  ----TSTQQNGRILGRPMEEVRRTYEFGRELGRGQFGVTYLVTHKETKQQVACKSIPTRR 120

Query: 191 LVNKEDIEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGH 250
           LV+K+DIEDVRREVQIMHHL+G  N V+LKGA+ED+HSV+L+MELC GGELFDRII+KG 
Sbjct: 121 LVHKDDIEDVRREVQIMHHLSGHRNIVDLKGAYEDRHSVNLIMELCEGGELFDRIISKGL 180

Query: 251 YTERAAASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQG 310
           Y+ERAAA L R +V +VH+CHSMGV+HRDLKPENFL L+K+E+SPLKATDFGLSVF+K G
Sbjct: 181 YSERAAADLCRQMVMVVHSCHSMGVMHRDLKPENFLFLSKDENSPLKATDFGLSVFFKPG 240

Query: 311 EVFKDIVGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRG 370
           + FKD+VGSAYY+APEVLKR YGPE DIWS GV+LYILL GVPPFW E+E GIF+AIL+G
Sbjct: 241 DKFKDLVGSAYYVAPEVLKRNYGPEADIWSAGVILYILLSGVPPFWGENETGIFDAILQG 300

Query: 371 HVDFTSDPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLN 430
            +DF++DPWP +S  AKDLVRKML  DPK RLTA++VL+HPWI+EDGEA D PLDNAVL+
Sbjct: 301 QLDFSADPWPALSDGAKDLVRKMLKYDPKDRLTAAEVLNHPWIREDGEASDKPLDNAVLS 360

Query: 431 RLKQFRAMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGT 490
           R+KQFRAMNK KK+AL+VIA  LSEEEI+GLK+MFK +DTDN+G +TLEEL+ GL K G+
Sbjct: 361 RMKQFRAMNKLKKMALKVIAENLSEEEIIGLKEMFKSLDTDNNGIVTLEELRTGLPKLGS 420

Query: 491 KLSEYEVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITT 550
           K+SE E++QLMEAAD DG+G+IDY EFI+ATMH+NR++RE+HLYTAFQ+FD DNSGYIT 
Sbjct: 421 KISEAEIRQLMEAADMDGDGSIDYLEFISATMHMNRIEREDHLYTAFQFFDNDNSGYITM 480

Query: 551 EELEQALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPKKRR 600
           EELE A+++Y M D + IKEI++EVD D DG+INY+EFVAMM+KGNPE  P +RR
Sbjct: 481 EELELAMKKYNMGDDKSIKEIIAEVDTDRDGKINYEEFVAMMKKGNPELVPNRRR 528

BLAST of CmaCh20G001150 vs. TAIR 10
Match: AT4G04720.1 (calcium-dependent protein kinase 21 )

HSP 1 Score: 674.5 bits (1739), Expect = 8.0e-194
Identity = 316/461 (68.55%), Postives = 392/461 (85.03%), Query Frame = 0

Query: 136 TVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKEDIEDVR 195
           T+LG+P ED+R  YS+GKELGRGQFG+T++C    TG   ACK+I KRKL++K+D EDV+
Sbjct: 67  TILGKPFEDIRKFYSLGKELGRGQFGITYMCKEIGTGNTYACKSILKRKLISKQDKEDVK 126

Query: 196 REVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERAAASLLR 255
           RE+QIM +L+GQPN VE+KGA+ED+ S+HLVMELCAGGELFDRIIA+GHY+ERAAA ++R
Sbjct: 127 REIQIMQYLSGQPNIVEIKGAYEDRQSIHLVMELCAGGELFDRIIAQGHYSERAAAGIIR 186

Query: 256 TIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKDIVGSAY 315
           +IV +V  CH MGV+HRDLKPENFLL +KEE++ LKATDFGLSVF ++G+V++DIVGSAY
Sbjct: 187 SIVNVVQICHFMGVVHRDLKPENFLLSSKEENAMLKATDFGLSVFIEEGKVYRDIVGSAY 246

Query: 316 YIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFTSDPWPT 375
           Y+APEVL+R YG E+DIWS GV+LYILL GVPPFWAE+E GIF+ +++G +DF S+PWP+
Sbjct: 247 YVAPEVLRRSYGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDEVIKGEIDFVSEPWPS 306

Query: 376 ISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQFRAMNKF 435
           IS +AKDLVRKML  DPK+R+TA+QVL HPWIK  GEAPD P+D+AVL+R+KQFRAMNK 
Sbjct: 307 ISESAKDLVRKMLTKDPKRRITAAQVLEHPWIK-GGEAPDKPIDSAVLSRMKQFRAMNKL 366

Query: 436 KKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEYEVKQLM 495
           KK+AL+VIA  LSEEEI GLK MF  +DTD SGTIT EELK GL + G++LSE EVKQLM
Sbjct: 367 KKLALKVIAESLSEEEIKGLKTMFANIDTDKSGTITYEELKTGLTRLGSRLSETEVKQLM 426

Query: 496 EAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQALREYG 555
           EAAD DGNGTIDY EFI+ATMH  ++DR+EH+Y AFQ+FDKDNSG+IT +ELE A++EYG
Sbjct: 427 EAADVDGNGTIDYYEFISATMHRYKLDRDEHVYKAFQHFDKDNSGHITRDELESAMKEYG 486

Query: 556 MHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPEANPK 597
           M D   IKE++SEVD DNDGRIN++EF AMMR G+ +   K
Sbjct: 487 MGDEASIKEVISEVDTDNDGRINFEEFCAMMRSGSTQPQGK 526

BLAST of CmaCh20G001150 vs. TAIR 10
Match: AT1G50700.1 (calcium-dependent protein kinase 33 )

HSP 1 Score: 666.8 bits (1719), Expect = 1.7e-191
Identity = 332/523 (63.48%), Postives = 418/523 (79.92%), Query Frame = 0

Query: 71  MGNCCSRENPDEPPTSVKDGSNADKSKDDGGNSNQGNST-ENPSNKAASSASPADAAKNT 130
           MGNC +++        +K   N ++S +     N+  ST ++PS  +  +  P       
Sbjct: 1   MGNCLAKKY----GLVMKPQQNGERSVE---IENRRRSTHQDPSKISTGTNQPPPWRNPA 60

Query: 131 KPSQIGTVLGRPMEDVRSIYSIGKELGRGQFGVTHLCTHKTTGEQLACKTIAKRKLVNKE 190
           K S    +L +P EDV+  Y++ KELGRGQFGVT+LCT K+TG++ ACK+I+K+KLV K 
Sbjct: 61  KHSGAAAILEKPYEDVKLFYTLSKELGRGQFGVTYLCTEKSTGKRFACKSISKKKLVTKG 120

Query: 191 DIEDVRREVQIMHHLTGQPNTVELKGAFEDKHSVHLVMELCAGGELFDRIIAKGHYTERA 250
           D ED+RRE+QIM HL+GQPN VE KGA+ED+ +V+LVMELCAGGELFDRI+AKGHY+ERA
Sbjct: 121 DKEDMRREIQIMQHLSGQPNIVEFKGAYEDEKAVNLVMELCAGGELFDRILAKGHYSERA 180

Query: 251 AASLLRTIVQIVHTCHSMGVIHRDLKPENFLLLNKEEDSPLKATDFGLSVFYKQGEVFKD 310
           AAS+ R IV +V+ CH MGV+HRDLKPENFLL +K+E + +KATDFGLSVF ++G V+KD
Sbjct: 181 AASVCRQIVNVVNICHFMGVMHRDLKPENFLLSSKDEKALIKATDFGLSVFIEEGRVYKD 240

Query: 311 IVGSAYYIAPEVLKRKYGPEVDIWSVGVMLYILLCGVPPFWAESEHGIFNAILRGHVDFT 370
           IVGSAYY+APEVLKR+YG E+DIWS G++LYILL GVPPFWAE+E GIF+AIL G +DF 
Sbjct: 241 IVGSAYYVAPEVLKRRYGKEIDIWSAGIILYILLSGVPPFWAETEKGIFDAILEGEIDFE 300

Query: 371 SDPWPTISPAAKDLVRKMLNSDPKQRLTASQVLSHPWIKEDGEAPDTPLDNAVLNRLKQF 430
           S PWP+IS +AKDLVR+ML  DPK+R++A++VL HPW++E GEA D P+D+AVL+R+KQF
Sbjct: 301 SQPWPSISNSAKDLVRRMLTQDPKRRISAAEVLKHPWLREGGEASDKPIDSAVLSRMKQF 360

Query: 431 RAMNKFKKVALRVIAGCLSEEEIMGLKQMFKGMDTDNSGTITLEELKQGLAKQGTKLSEY 490
           RAMNK KK+AL+VIA  +  EEI GLK MF  +DTDNSGTIT EELK+GLAK G++L+E 
Sbjct: 361 RAMNKLKKLALKVIAENIDTEEIQGLKAMFANIDTDNSGTITYEELKEGLAKLGSRLTEA 420

Query: 491 EVKQLMEAADADGNGTIDYDEFITATMHLNRMDREEHLYTAFQYFDKDNSGYITTEELEQ 550
           EVKQLM+AAD DGNG+IDY EFITATMH +R++  E++Y AFQ+FDKD SGYITT+ELE 
Sbjct: 421 EVKQLMDAADVDGNGSIDYIEFITATMHRHRLESNENVYKAFQHFDKDGSGYITTDELEA 480

Query: 551 ALREYGMHDGRDIKEILSEVDADNDGRINYDEFVAMMRKGNPE 593
           AL+EYGM D   IKEILS+VDADNDGRINYDEF AMMR GNP+
Sbjct: 481 ALKEYGMGDDATIKEILSDVDADNDGRINYDEFCAMMRSGNPQ 516

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMP52.2e-25281.90Calcium-dependent protein kinase 17 OS=Arabidopsis thaliana OX=3702 GN=CPK17 PE=... [more]
Q3E9C05.3e-25181.39Calcium-dependent protein kinase 34 OS=Arabidopsis thaliana OX=3702 GN=CPK34 PE=... [more]
Q5VQQ54.0e-24679.40Calcium-dependent protein kinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
B9FKW91.1e-24077.72Calcium-dependent protein kinase 14 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q2RAV09.2e-22772.57Calcium-dependent protein kinase 25 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1JAC99.9e-309100.00calcium-dependent protein kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111483... [more]
A0A6J1FT918.4e-30899.62calcium-dependent protein kinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1H1602.5e-29187.16calcium-dependent protein kinase 34-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1DHQ11.0e-28993.81calcium-dependent protein kinase 2-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A1S3BMH21.8e-28994.02calcium-dependent protein kinase 34 OS=Cucumis melo OX=3656 GN=LOC103491467 PE=4... [more]
Match NameE-valueIdentityDescription
XP_022986171.12.0e-308100.00calcium-dependent protein kinase 2-like [Cucurbita maxima] >XP_023512839.1 calci... [more]
XP_022943517.11.7e-30799.62calcium-dependent protein kinase 2-like [Cucurbita moschata][more]
KAG6570423.15.1e-30799.44Calcium-dependent protein kinase 17, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022958182.15.1e-29187.16calcium-dependent protein kinase 34-like [Cucurbita moschata] >KAG7035889.1 Calc... [more]
XP_038902029.11.6e-28994.19calcium-dependent protein kinase 2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G12180.11.5e-25381.90calcium-dependent protein kinase 17 [more]
AT5G19360.13.8e-25281.39calcium-dependent protein kinase 34 [more]
AT4G23650.18.0e-20264.67calcium-dependent protein kinase 6 [more]
AT4G04720.18.0e-19468.55calcium-dependent protein kinase 21 [more]
AT1G50700.11.7e-19163.48calcium-dependent protein kinase 33 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 472..492
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 235..414
e-value: 9.4E-60
score: 203.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 151..234
e-value: 1.3E-28
score: 101.1
NoneNo IPR availableGENE3D1.10.238.10coord: 415..591
e-value: 2.4E-59
score: 201.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..135
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 74..139
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 98..595
NoneNo IPR availablePANTHERPTHR24349:SF293CALMODULIN-DOMAIN KINASE CDPK PROTEINcoord: 98..595
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 149..406
e-value: 8.32779E-137
score: 397.232
IPR002048EF-hand domainSMARTSM00054efh_1coord: 561..589
e-value: 1.2E-6
score: 38.0
coord: 526..554
e-value: 0.026
score: 23.7
coord: 454..482
e-value: 3.5E-5
score: 33.2
coord: 490..518
e-value: 1.6E-5
score: 34.4
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 524..587
e-value: 1.1E-14
score: 54.5
coord: 455..515
e-value: 5.1E-12
score: 46.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 561..592
score: 12.449146
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 522..557
score: 15.322372
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 486..521
score: 11.02648
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 450..485
score: 14.150764
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 149..407
e-value: 6.5E-98
score: 341.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 149..407
e-value: 2.1E-74
score: 250.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 149..407
score: 47.523468
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 269..281
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 499..511
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 570..582
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 535..547
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 463..475
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 155..182
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 447..588
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 135..431

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G001150.1CmaCh20G001150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity