CmaCh20G000770 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh20G000770
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	E3 ubiquitin ligase BIG BROTHER-like
Location	Cma_Chr20: 336885 .. 337511 (+)
RNA-Seq Expression	CmaCh20G000770
Synteny	CmaCh20G000770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGGAGCGCAGAGATTGGGTCAAAAAACAGATGCTCCAACTTCAGGAGCAATGTATGAGCTTCCAGGCTCTATCTGTTGAACTTGCAGTAAGACATTTTTCTATATACTGAGATAGAGTTGAGTATAGGCCGTAATAATGCGGGGAATCAAACAAAATATTTCTTATGAGGGTGTAGAAACCTCTCCCTAATAAATGCGTTTTAAAAACAGTAAAGCTGACGCTATATGTAATAGGCCAAAATAGATAATTTGTGTTAGCGGTGGGCTTTTGCCTTGTTTTCTAGTTCCATGCTCTGTAACTCATGTGTTGTTACATCTGATGGTATCAGTCTTGATTTGTATATAGGTGTGTGATATGCGAAATGGAATACAAATGTGGAGACCAAAGGATTACTCTACCATGCAAACATAAGTATCATACCAGTTGCAGGACCAAGTGGCTTAGTATGAACAAGGTGATGTTCTGATATATTTTATCCTTGCACTTTTGGGGGTAATTCTAGTAACAGACTATCAGTAACTTTTGTCTATACAGTACTTAATATTTCTGTTCCAGGCATGCCCCATTTGCTACACTGAGGTTTTTGGTGACACTTCAAAACCATATATTACTAGAGAATAG

mRNA sequence

ATGGGAGGAGCGCAGAGATTGGGTCAAAAAACAGATGCTCCAACTTCAGGAGCAATGTGTGTGATATGCGAAATGGAATACAAATGTGGAGACCAAAGGATTACTCTACCATGCAAACATAAGTATCATACCAGTTGCAGGACCAAGTGGCTTAGTATGAACAAGGCATGCCCCATTTGCTACACTGAGGTTTTTGGTGACACTTCAAAACCATATATTACTAGAGAATAG

Coding sequence (CDS)

ATGGGAGGAGCGCAGAGATTGGGTCAAAAAACAGATGCTCCAACTTCAGGAGCAATGTGTGTGATATGCGAAATGGAATACAAATGTGGAGACCAAAGGATTACTCTACCATGCAAACATAAGTATCATACCAGTTGCAGGACCAAGTGGCTTAGTATGAACAAGGCATGCCCCATTTGCTACACTGAGGTTTTTGGTGACACTTCAAAACCATATATTACTAGAGAATAG

Protein sequence

MGGAQRLGQKTDAPTSGAMCVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTSKPYITRE

Homology

[Close]

BLAST of CmaCh20G000770 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 1.4e-15
Identity = 30/54 (55.56%), Postives = 44/54 (81.48%), Query Frame = 0


Query: 16  SGAMCVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTS 70
           +G  CVIC+++YK G++++ LPCKH YH+ C +KWLS+NK CP+C +EVFG+ S
Sbjct: 193 AGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFGEPS 246

[Close]

BLAST of CmaCh20G000770 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.0e-10
Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 0


Query: 20  CVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTS 70
           CVIC ++Y+  +  I LPCKH YH+ C   WL +NK CP+C  EV   TS
Sbjct: 288 CVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

[Close]

BLAST of CmaCh20G000770 vs. ExPASy Swiss-Prot
Match: P87139 (Uncharacterized RING finger protein C57A7.09 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC57A7.09 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 3.7e-08
Identity = 25/59 (42.37%), Postives = 33/59 (55.93%), Query Frame = 0


Query: 7   LGQKTDAPTSGAMCVICEMEYKCGDQRITLPCKHKYHTSCRTKWL-SMNKACPICYTEV 65
           + + T   T G  CVIC   +  GD+ + LPCKH++H  C  KW+     ACP C TEV
Sbjct: 308 MDESTRRATFGVECVICLESFTKGDKVVALPCKHEFHRPCIAKWIVDYRHACPTCNTEV 366

[Close]

BLAST of CmaCh20G000770 vs. ExPASy Swiss-Prot
Match: Q69U49 (Receptor homology region, transmembrane domain- and RING domain-containing protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=RMR1 PE=2 SV=2)

HSP 1 Score: 56.2 bits (134), Expect = 1.8e-07
Identity = 22/58 (37.93%), Postives = 34/58 (58.62%), Query Frame = 0


Query: 15  TSGAMCVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKA-CPICYTEVFGDTSKP 72
           ++ + C IC  +Y  G++   LPC+HK+H +C   WL+  K  CP+C  +    TSKP
Sbjct: 231 STSSSCAICLEDYSFGEKLRVLPCRHKFHATCVDMWLTSWKTFCPVCKRDASAGTSKP 288

[Close]

BLAST of CmaCh20G000770 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 9.1e-07
Identity = 20/52 (38.46%), Postives = 28/52 (53.85%), Query Frame = 0


Query: 11  TDAPTSGAMCVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYT 63
           T +P     C +C+ EY  G+   TL C H++H+ C  +WL     CPIC T
Sbjct: 628 TKSPQDAEPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQKNLCPICKT 679

[Close]

BLAST of CmaCh20G000770 vs. ExPASy TrEMBL
Match: A0A6J1D445 (E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017416 PE=4 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 9.8e-20
Identity = 44/51 (86.27%), Postives = 47/51 (92.16%), Query Frame = 0


Query: 20  CVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTSK 71
           CVIC+MEYK GDQRITLPCKH+YHT C TKWLS+NKACPICYTEVFG TSK
Sbjct: 208 CVICQMEYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSK 258

[Close]

BLAST of CmaCh20G000770 vs. ExPASy TrEMBL
Match: A0A6J1H1S6 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC111459339 PE=4 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 9.8e-20
Identity = 44/51 (86.27%), Postives = 47/51 (92.16%), Query Frame = 0


Query: 20  CVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTSK 71
           CVIC+M YK GDQRITLPCKHKYHTSC +KWLS+NKACPICYTEVFGD SK
Sbjct: 193 CVICQMNYKHGDQRITLPCKHKYHTSCGSKWLSINKACPICYTEVFGDISK 243

[Close]

BLAST of CmaCh20G000770 vs. ExPASy TrEMBL
Match: A0A6J1D6M7 (E3 ubiquitin ligase BIG BROTHER-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017416 PE=4 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 9.8e-20
Identity = 44/51 (86.27%), Postives = 47/51 (92.16%), Query Frame = 0


Query: 20  CVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTSK 71
           CVIC+MEYK GDQRITLPCKH+YHT C TKWLS+NKACPICYTEVFG TSK
Sbjct: 196 CVICQMEYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSK 246

[Close]

BLAST of CmaCh20G000770 vs. ExPASy TrEMBL
Match: A0A6J1DMS5 (E3 ubiquitin ligase BIG BROTHER-like OS=Momordica charantia OX=3673 GN=LOC111021453 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.7e-19
Identity = 44/54 (81.48%), Postives = 47/54 (87.04%), Query Frame = 0


Query: 17  GAMCVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTSK 71
           G  CVIC+MEYK GD+RITLPCKH YH  C TKWLS+NKACPICYTEVFGDTSK
Sbjct: 196 GERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSVNKACPICYTEVFGDTSK 249

[Close]

BLAST of CmaCh20G000770 vs. ExPASy TrEMBL
Match: A0A6J1EIF5 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC111434637 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.7e-19
Identity = 44/54 (81.48%), Postives = 47/54 (87.04%), Query Frame = 0


Query: 17  GAMCVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTSK 71
           G  CVIC+MEYK GD+RITLPCKH YH  C TKWLS+NKACPICYTEVFGDTSK
Sbjct: 193 GERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTEVFGDTSK 246

[Close]

BLAST of CmaCh20G000770 vs. NCBI nr
Match: XP_038901510.1 (E3 ubiquitin-protein ligase BIG BROTHER-like [Benincasa hispida])

HSP 1 Score: 108.6 bits (270), Expect = 2.4e-20
Identity = 45/51 (88.24%), Postives = 49/51 (96.08%), Query Frame = 0


Query: 20  CVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTSK 71
           CVIC+MEYK GDQRITLPCKH+YHTSC TKWLS+NKACPICYTEVFGD+SK
Sbjct: 191 CVICQMEYKRGDQRITLPCKHRYHTSCGTKWLSINKACPICYTEVFGDSSK 241

[Close]

BLAST of CmaCh20G000770 vs. NCBI nr
Match: XP_023532670.1 (E3 ubiquitin ligase BIG BROTHER-like [Cucurbita pepo subsp. pepo] >XP_023532671.1 E3 ubiquitin ligase BIG BROTHER-like [Cucurbita pepo subsp. pepo] >XP_023532672.1 E3 ubiquitin ligase BIG BROTHER-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 105.9 bits (263), Expect = 1.5e-19
Identity = 44/51 (86.27%), Postives = 47/51 (92.16%), Query Frame = 0


Query: 20  CVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTSK 71
           CVIC+M YK GDQRITLPCKHKYHTSC +KWLS+NKACPICYTEVFGD SK
Sbjct: 193 CVICQMNYKYGDQRITLPCKHKYHTSCGSKWLSINKACPICYTEVFGDISK 243

[Close]

BLAST of CmaCh20G000770 vs. NCBI nr
Match: XP_022957965.1 (E3 ubiquitin ligase BIG BROTHER-like [Cucurbita moschata] >XP_022957966.1 E3 ubiquitin ligase BIG BROTHER-like [Cucurbita moschata] >XP_022957967.1 E3 ubiquitin ligase BIG BROTHER-like [Cucurbita moschata] >XP_022957968.1 E3 ubiquitin ligase BIG BROTHER-like [Cucurbita moschata] >KAG7035855.1 E3 ubiquitin ligase BIG BROTHER [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 105.5 bits (262), Expect = 2.0e-19
Identity = 44/51 (86.27%), Postives = 47/51 (92.16%), Query Frame = 0


Query: 20  CVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTSK 71
           CVIC+M YK GDQRITLPCKHKYHTSC +KWLS+NKACPICYTEVFGD SK
Sbjct: 193 CVICQMNYKHGDQRITLPCKHKYHTSCGSKWLSINKACPICYTEVFGDISK 243

[Close]

BLAST of CmaCh20G000770 vs. NCBI nr
Match: XP_022148861.1 (E3 ubiquitin ligase BIG BROTHER-like isoform X2 [Momordica charantia])

HSP 1 Score: 105.5 bits (262), Expect = 2.0e-19
Identity = 44/51 (86.27%), Postives = 47/51 (92.16%), Query Frame = 0


Query: 20  CVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTSK 71
           CVIC+MEYK GDQRITLPCKH+YHT C TKWLS+NKACPICYTEVFG TSK
Sbjct: 196 CVICQMEYKRGDQRITLPCKHRYHTCCGTKWLSINKACPICYTEVFGGTSK 246

[Close]

BLAST of CmaCh20G000770 vs. NCBI nr
Match: KAG6605908.1 (E3 ubiquitin-protein ligase BIG BROTHER, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 105.5 bits (262), Expect = 2.0e-19
Identity = 44/51 (86.27%), Postives = 47/51 (92.16%), Query Frame = 0


Query: 20  CVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTSK 71
           CVIC+M YK GDQRITLPCKHKYHTSC +KWLS+NKACPICYTEVFGD SK
Sbjct: 264 CVICQMNYKHGDQRITLPCKHKYHTSCGSKWLSINKACPICYTEVFGDISK 314

[Close]

BLAST of CmaCh20G000770 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 1.0e-16
Identity = 30/54 (55.56%), Postives = 44/54 (81.48%), Query Frame = 0


Query: 16  SGAMCVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTS 70
           +G  CVIC+++YK G++++ LPCKH YH+ C +KWLS+NK CP+C +EVFG+ S
Sbjct: 193 AGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFGEPS 246

[Close]

BLAST of CmaCh20G000770 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 1.0e-16
Identity = 30/54 (55.56%), Postives = 44/54 (81.48%), Query Frame = 0


Query: 16  SGAMCVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTS 70
           +G  CVIC+++YK G++++ LPCKH YH+ C +KWLS+NK CP+C +EVFG+ S
Sbjct: 193 AGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFGEPS 246

[Close]

BLAST of CmaCh20G000770 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 7.4e-12
Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 0


Query: 20  CVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVFGDTS 70
           CVIC ++Y+  +  I LPCKH YH+ C   WL +NK CP+C  EV   TS
Sbjct: 288 CVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

[Close]

BLAST of CmaCh20G000770 vs. TAIR 10
Match: AT5G52140.1 (RING/U-box superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 6.3e-11
Identity = 26/48 (54.17%), Postives = 31/48 (64.58%), Query Frame = 0


Query: 18  AMCVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYTEVF 66
           + C IC MEY  GD+  TLPCKH YH  C ++WL  NK C IC  EV+
Sbjct: 232 SQCSICLMEYAKGDKITTLPCKHIYHKDCISQWLKQNKVCCICKAEVY 279

[Close]

BLAST of CmaCh20G000770 vs. TAIR 10
Match: AT5G24870.1 (RING/U-box superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 2.4e-10
Identity = 25/43 (58.14%), Postives = 29/43 (67.44%), Query Frame = 0


Query: 20  CVICEMEYKCGDQRITLPCKHKYHTSCRTKWLSMNKACPICYT 63
           C IC+ EY  GD+  TLPC+HKYH SC  +WL M   CPIC T
Sbjct: 468 CSICQEEYVDGDEVGTLPCQHKYHVSCAQQWLRMKNWCPICKT 510

The following BLAST results are available for this feature:

BLAST of CmaCh20G000770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L649	1.4e-15	55.56	E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE...	[more]
Q9LT17	1.0e-10	50.00	E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P...	[more]
P87139	3.7e-08	42.37	Uncharacterized RING finger protein C57A7.09 OS=Schizosaccharomyces pombe (strai...	[more]
Q69U49	1.8e-07	37.93	Receptor homology region, transmembrane domain- and RING domain-containing prote...	[more]
Q9FMM4	9.1e-07	38.46	Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]

BLAST of CmaCh20G000770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D445	9.8e-20	86.27	E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1H1S6	9.8e-20	86.27	E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC1114593...	[more]
A0A6J1D6M7	9.8e-20	86.27	E3 ubiquitin ligase BIG BROTHER-like isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DMS5	1.7e-19	81.48	E3 ubiquitin ligase BIG BROTHER-like OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A6J1EIF5	1.7e-19	81.48	E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC1114346...	[more]

BLAST of CmaCh20G000770 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901510.1	2.4e-20	88.24	E3 ubiquitin-protein ligase BIG BROTHER-like [Benincasa hispida]	[more]
XP_023532670.1	1.5e-19	86.27	E3 ubiquitin ligase BIG BROTHER-like [Cucurbita pepo subsp. pepo] >XP_023532671....	[more]
XP_022957965.1	2.0e-19	86.27	E3 ubiquitin ligase BIG BROTHER-like [Cucurbita moschata] >XP_022957966.1 E3 ubi...	[more]
XP_022148861.1	2.0e-19	86.27	E3 ubiquitin ligase BIG BROTHER-like isoform X2 [Momordica charantia]	[more]
KAG6605908.1	2.0e-19	86.27	E3 ubiquitin-protein ligase BIG BROTHER, partial [Cucurbita argyrosperma subsp. ...	[more]

BLAST of CmaCh20G000770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63530.1	1.0e-16	55.56	RING/U-box superfamily protein	[more]
AT3G63530.2	1.0e-16	55.56	RING/U-box superfamily protein	[more]
AT3G19910.1	7.4e-12	50.00	RING/U-box superfamily protein	[more]
AT5G52140.1	6.3e-11	54.17	RING/U-box superfamily protein	[more]
AT5G24870.1	2.4e-10	58.14	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 20..60 e-value: 5.0E-4 score: 29.4
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 19..60 e-value: 8.3E-10 score: 38.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 20..60 score: 10.895954
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 4..68 e-value: 2.4E-15 score: 57.5
IPR033276	E3 ubiquitin-protein ligase BIG BROTHER	PANTHER	PTHR46400	RING/U-BOX SUPERFAMILY PROTEIN	coord: 17..70
None	No IPR available	PANTHER	PTHR46400:SF5	RING/U-BOX SUPERFAMILY PROTEIN	coord: 17..70
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 17..67

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh20G000770.1	CmaCh20G000770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046621	negative regulation of organ growth
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity