CmaCh20G000560 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh20G000560
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Delta(12)-fatty-acid desaturase
Location	Cma_Chr20: 248698 .. 249152 (-)
RNA-Seq Expression	CmaCh20G000560
Synteny	CmaCh20G000560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGTTGGAAGCAAAATGGAAAAGGCGGTCCATGAGCGAGTTCCACACGCAAAGCCTCCTTTCACTCTCAGCCAAATCAAGAAAGCCATTCCTCCCCACTGCTTCCACCGCTCTCTTCTCCGCACCTTCTCCTGCTTCTTTTTCTTTTTTTCCCCATTGAAGGAGTCGTCTGGTACCGCAACAAGCTCTGAGTCTGTCCCGAATAAAAACTGAAGCCCACCAAAGGGAAAAATGGAAGATCAAAATGGTGGTATCTCGCAGTTTCTTTTTCTTTTTCTTTGCTCTTCTTCCTTTTATGGTCTGTCTGTACGTATTTGAAGAAAGACCCGTAGTGTTCTACTGGTGATGAAGGATGAACCTTTTTAATCCCTTAATTAAGGTCAATTTTTATTGGTGGGTTTACAATATATATCAGTTATGTTGTTGCTAATTCTATTCATTTGATTAACTAA

mRNA sequence

ATGGGAGTTGGAAGCAAAATGGAAAAGGCGGTCCATGAGCGAGTTCCACACGCAAAGCCTCCTTTCACTCTCAGCCAAATCAAGAAAGCCATTCCTCCCCACTGCTTCCACCGCTCTCTTCTCCGCACCTTCTCCTGCTTCTTTTTCTTTTTTTCCCCATTGAAGGAGTCGTCTGGTACCGCAACAAGCTCTGAGTCAATTTTTATTGGTGGGTTTACAATATATATCAGTTATGTTGTTGCTAATTCTATTCATTTGATTAACTAA

Coding sequence (CDS)

ATGGGAGTTGGAAGCAAAATGGAAAAGGCGGTCCATGAGCGAGTTCCACACGCAAAGCCTCCTTTCACTCTCAGCCAAATCAAGAAAGCCATTCCTCCCCACTGCTTCCACCGCTCTCTTCTCCGCACCTTCTCCTGCTTCTTTTTCTTTTTTTCCCCATTGAAGGAGTCGTCTGGTACCGCAACAAGCTCTGAGTCAATTTTTATTGGTGGGTTTACAATATATATCAGTTATGTTGTTGCTAATTCTATTCATTTGATTAACTAA

Protein sequence

MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFFFFFSPLKESSGTATSSESIFIGGFTIYISYVVANSIHLIN

Homology

[Close]

BLAST of CmaCh20G000560 vs. ExPASy Swiss-Prot
Match: Q84UB9 (Delta(12)-oleate desaturase OS=Trichosanthes kirilowii OX=3677 GN=FAD2 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.2e-13
Identity = 34/39 (87.18%), Postives = 36/39 (92.31%), Query Frame = 0


Query: 7  MEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          MEK V ERVPHAKPPFTLSQIKK +PPHCFHRSLLR+FS
Sbjct: 1  MEKGVQERVPHAKPPFTLSQIKKVVPPHCFHRSLLRSFS 39

[Close]

BLAST of CmaCh20G000560 vs. ExPASy Swiss-Prot
Match: Q8GZC3 (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.6e-10
Identity = 29/40 (72.50%), Postives = 35/40 (87.50%), Query Frame = 0


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K+E  V +RVPH+KPPFTL Q+KKAIPPHCF RS+LR+FS
Sbjct: 15 KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFS 54

[Close]

BLAST of CmaCh20G000560 vs. ExPASy Swiss-Prot
Match: Q9AT72 (Delta(12) fatty acid desaturase FAD2 OS=Calendula officinalis OX=41496 GN=FAD2 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.0e-09
Identity = 28/46 (60.87%), Postives = 36/46 (78.26%), Query Frame = 0


Query: 4  GSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFFF 50
          G+K E    +RVPH KPPFT+  IKKAIPPHCF+RS++R+FS  F+
Sbjct: 14 GNKTEPEPIQRVPHEKPPFTVGDIKKAIPPHCFNRSVIRSFSYVFY 59

[Close]

BLAST of CmaCh20G000560 vs. ExPASy Swiss-Prot
Match: Q84VT2 (Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 1.7e-09
Identity = 29/44 (65.91%), Postives = 35/44 (79.55%), Query Frame = 0


Query: 2  GVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          G G +  +   +RVP AKPPFTLS+IKKAIPPHCF RSLL++FS
Sbjct: 15 GEGDEKSQKPVQRVPSAKPPFTLSEIKKAIPPHCFKRSLLKSFS 58

[Close]

BLAST of CmaCh20G000560 vs. ExPASy Swiss-Prot
Match: Q84UC0 (Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes kirilowii OX=3677 GN=FAC PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 3.0e-09
Identity = 30/47 (63.83%), Postives = 35/47 (74.47%), Query Frame = 0


Query: 5  SKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFFFFF 52
          S  + A  ER+ HAKPPF++SQIKKAIPPHCF RSL R+FS   F F
Sbjct: 14 SSTKLAFGERITHAKPPFSISQIKKAIPPHCFQRSLYRSFSYVIFDF 60

[Close]

BLAST of CmaCh20G000560 vs. ExPASy TrEMBL
Match: A0A6J1FSC4 (delta(12)-oleate desaturase OS=Cucurbita moschata OX=3662 GN=LOC111448353 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.4e-17
Identity = 45/46 (97.83%), Postives = 46/46 (100.00%), Query Frame = 0


Query: 1  MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSC 47
          MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLR+FSC
Sbjct: 1  MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSC 46

[Close]

BLAST of CmaCh20G000560 vs. ExPASy TrEMBL
Match: A0A6J1H0J3 (delta(12)-oleate desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111459302 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 2.3e-12
Identity = 35/39 (89.74%), Postives = 38/39 (97.44%), Query Frame = 0


Query: 8  EKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSC 47
          EK VHERVPHAKPPFTL+QIKKAIPPHCFHRSLLR+F+C
Sbjct: 3  EKGVHERVPHAKPPFTLTQIKKAIPPHCFHRSLLRSFTC 41

[Close]

BLAST of CmaCh20G000560 vs. ExPASy TrEMBL
Match: A0A6J1K4R6 (delta(12)-oleate desaturase OS=Cucurbita maxima OX=3661 GN=LOC111490747 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 6.6e-12
Identity = 34/39 (87.18%), Postives = 37/39 (94.87%), Query Frame = 0


Query: 8  EKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSC 47
          EK  HERVPHAKPPFTL+QIKKAIPPHCFHRSLLR+F+C
Sbjct: 3  EKGTHERVPHAKPPFTLTQIKKAIPPHCFHRSLLRSFTC 41

[Close]

BLAST of CmaCh20G000560 vs. ExPASy TrEMBL
Match: A0A7J6FWL1 (Uncharacterized protein (Fragment) OS=Cannabis sativa OX=3483 GN=G4B88_004708 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.8e-10
Identity = 35/53 (66.04%), Postives = 43/53 (81.13%), Query Frame = 0


Query: 4   GSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFF--FFFSPL 55
           GSK + ++ ERVPH KPPFTLS+IKKAIPPHCF RSL+R+F+C F   FF+ L
Sbjct: 435 GSKNKNSLIERVPHTKPPFTLSEIKKAIPPHCFKRSLIRSFACVFHDLFFASL 487

[Close]

BLAST of CmaCh20G000560 vs. ExPASy TrEMBL
Match: A0A803PX48 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.8e-10
Identity = 35/53 (66.04%), Postives = 43/53 (81.13%), Query Frame = 0


Query: 4  GSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFF--FFFSPL 55
          GSK + ++ ERVPH KPPFTLS+IKKAIPPHCF RSL+R+F+C F   FF+ L
Sbjct: 16 GSKNKNSLIERVPHTKPPFTLSEIKKAIPPHCFKRSLIRSFACVFHDLFFASL 68

[Close]

BLAST of CmaCh20G000560 vs. NCBI nr
Match: XP_023512996.1 (delta(12)-oleate desaturase [Cucurbita pepo subsp. pepo] >XP_023522472.1 delta(12)-oleate desaturase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 98.6 bits (244), Expect = 2.9e-17
Identity = 45/46 (97.83%), Postives = 46/46 (100.00%), Query Frame = 0


Query: 1  MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSC 47
          MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLR+FSC
Sbjct: 1  MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSC 46

[Close]

BLAST of CmaCh20G000560 vs. NCBI nr
Match: KAG6570369.1 (Delta(12)-oleate desaturase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 98.6 bits (244), Expect = 2.9e-17
Identity = 45/46 (97.83%), Postives = 46/46 (100.00%), Query Frame = 0


Query: 1  MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSC 47
          MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLR+FSC
Sbjct: 1  MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSC 46

[Close]

BLAST of CmaCh20G000560 vs. NCBI nr
Match: XP_022943671.1 (delta(12)-oleate desaturase [Cucurbita moschata])

HSP 1 Score: 98.6 bits (244), Expect = 2.9e-17
Identity = 45/46 (97.83%), Postives = 46/46 (100.00%), Query Frame = 0


Query: 1  MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSC 47
          MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLR+FSC
Sbjct: 1  MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSC 46

[Close]

BLAST of CmaCh20G000560 vs. NCBI nr
Match: XP_022957911.1 (delta(12)-oleate desaturase-like [Cucurbita moschata] >XP_023533966.1 delta(12)-oleate desaturase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 81.3 bits (199), Expect = 4.7e-12
Identity = 35/39 (89.74%), Postives = 38/39 (97.44%), Query Frame = 0


Query: 8  EKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSC 47
          EK VHERVPHAKPPFTL+QIKKAIPPHCFHRSLLR+F+C
Sbjct: 3  EKGVHERVPHAKPPFTLTQIKKAIPPHCFHRSLLRSFTC 41

[Close]

BLAST of CmaCh20G000560 vs. NCBI nr
Match: KAG6605886.1 (Delta(12)-oleate desaturase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 81.3 bits (199), Expect = 4.7e-12
Identity = 35/39 (89.74%), Postives = 38/39 (97.44%), Query Frame = 0


Query: 8   EKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSC 47
           EK VHERVPHAKPPFTL+QIKKAIPPHCFHRSLLR+F+C
Sbjct: 68  EKGVHERVPHAKPPFTLTQIKKAIPPHCFHRSLLRSFTC 106

[Close]

BLAST of CmaCh20G000560 vs. TAIR 10
Match: AT3G12120.1 (fatty acid desaturase 2 )

HSP 1 Score: 51.2 bits (121), Expect = 4.9e-07
Identity = 23/40 (57.50%), Postives = 29/40 (72.50%), Query Frame = 0


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K E    +RVP  KPPF++  +KKAIPPHCF RS+ R+FS
Sbjct: 15 KSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFS 54

[Close]

BLAST of CmaCh20G000560 vs. TAIR 10
Match: AT3G12120.2 (fatty acid desaturase 2 )

HSP 1 Score: 51.2 bits (121), Expect = 4.9e-07
Identity = 23/40 (57.50%), Postives = 29/40 (72.50%), Query Frame = 0


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K E    +RVP  KPPF++  +KKAIPPHCF RS+ R+FS
Sbjct: 15 KSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFS 54

The following BLAST results are available for this feature:

BLAST of CmaCh20G000560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84UB9	1.2e-13	87.18	Delta(12)-oleate desaturase OS=Trichosanthes kirilowii OX=3677 GN=FAD2 PE=2 SV=1	[more]
Q8GZC3	1.6e-10	72.50	Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV...	[more]
Q9AT72	1.0e-09	60.87	Delta(12) fatty acid desaturase FAD2 OS=Calendula officinalis OX=41496 GN=FAD2 P...	[more]
Q84VT2	1.7e-09	65.91	Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2	[more]
Q84UC0	3.0e-09	63.83	Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes k...	[more]

BLAST of CmaCh20G000560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FSC4	1.4e-17	97.83	delta(12)-oleate desaturase OS=Cucurbita moschata OX=3662 GN=LOC111448353 PE=3 S...	[more]
A0A6J1H0J3	2.3e-12	89.74	delta(12)-oleate desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111459302 P...	[more]
A0A6J1K4R6	6.6e-12	87.18	delta(12)-oleate desaturase OS=Cucurbita maxima OX=3661 GN=LOC111490747 PE=4 SV=...	[more]
A0A7J6FWL1	4.8e-10	66.04	Uncharacterized protein (Fragment) OS=Cannabis sativa OX=3483 GN=G4B88_004708 PE...	[more]
A0A803PX48	4.8e-10	66.04	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]

BLAST of CmaCh20G000560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023512996.1	2.9e-17	97.83	delta(12)-oleate desaturase [Cucurbita pepo subsp. pepo] >XP_023522472.1 delta(1...	[more]
KAG6570369.1	2.9e-17	97.83	Delta(12)-oleate desaturase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022943671.1	2.9e-17	97.83	delta(12)-oleate desaturase [Cucurbita moschata]	[more]
XP_022957911.1	4.7e-12	89.74	delta(12)-oleate desaturase-like [Cucurbita moschata] >XP_023533966.1 delta(12)-...	[more]
KAG6605886.1	4.7e-12	89.74	Delta(12)-oleate desaturase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmaCh20G000560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12120.1	4.9e-07	57.50	fatty acid desaturase 2	[more]
AT3G12120.2	4.9e-07	57.50	fatty acid desaturase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR32100	OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC	coord: 1..52
None	No IPR available	PANTHER	PTHR32100:SF40	OLEATE HYDROXYLASE FAH12	coord: 1..52

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh20G000560.1	CmaCh20G000560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006636	unsaturated fatty acid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0102985	delta12-fatty-acid desaturase activity
molecular_function	GO:0102987	palmitoleic acid delta 12 desaturase activity