CmaCh20G000380 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G000380
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionBifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein, putative
LocationCma_Chr20: 164000 .. 165229 (-)
RNA-Seq ExpressionCmaCh20G000380
SyntenyCmaCh20G000380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCCTTTATTTCCCATCCGCCATATCACACACCATAAAGCCCCCACTCCCCTTCCTCCCCATCCATTCTGCCGCGCATTGCCATCCCTCCCAACCAAAATCATGGCCTCCAAATCCTCTTCAACTAATTATACGGCTCTCATCTCATGCGCCATCGCGCTCCTTTTTGTGGGGTTTGTGAGCTCCAACGTAGACCAGGACAGGGCAGAGTGCGCGGACCAGGTGGTTGGGCTGGCCACTTGCTTGCCGTACGTGGGCGGGGAGGCGAGAGCACCGACGCCGGACTGTTGCAGTGGGCTCAAGGTTGTGCTTGAGAAGAGTAAGAAATGTCTTTGTGTGTTGATAAAGGACCGTAATGATCCAAGCCTGGGACTTAAAGTTAATCTTACGCTTGCTCTTGGACTGCCAAATGCATGCCATGCCCCTGCCAACATCCAAGACTGCATCGGTATATTTCATCGTTATTATTCCTGTGGCTTTAGTTTGAATTTTGATGCACAATTAATCCGAAAATTAAACATAATATACAAGTTTATGTATTCTTGTTTTTGGTAATTGAGTATGTGGGTTATAGGGCTTCTCCATCTGTCGCCCAACTCCCAAGATGCTAAGGATTTTATGGGCTCCAAGGGAGCTAACACTACAGCCGCACCTGCTACTGCTGGTAATCCATATTTGTGTTTATCTCTTAATTATTTATTAAAGCGTATTTCTAAATGTTTATTAAAATAATGGTTTGCAGCGCCTGGGAATTCCACCCAAACTCCCATGGAGAAAGGTAGCGGAATGAGGAATCAATGGTTGGGTGCGGAGATGTTCATTGCGTTTCTCACTTCATCGCTTCTATTCACTTGTGTTTGGTAGTTCTATTTTAACCTTTTGTTCTTCCCTAATGTCCCAAAAATGTGAACCATCATATATGTGCTAAACTAATTGAAGTATGATTAAATGGTTAAAAGTTTTATTCTCATGGAGTTACAAATGTATATTTATATAAGGAACTGGCCTAGGATTTATCATCTCTCCTTCATAAATTAAGTTGAAAAACAACTAAATCGGACTCACTAAGGGTCTCCTAGGTTGTAGGCGTGCATAACGCACAACCCATTTGACAAACAGCCGTAATTCCTTTCTTCTCACAACCGACTATCTACTGTCTCGTCTCAAAGTTCGAGTGTTTTGCCTTCATCTTCATCTTATTTTACCCCCCTCCTTCTAAACATCTAAAAT

mRNA sequence

CTTCCTTTATTTCCCATCCGCCATATCACACACCATAAAGCCCCCACTCCCCTTCCTCCCCATCCATTCTGCCGCGCATTGCCATCCCTCCCAACCAAAATCATGGCCTCCAAATCCTCTTCAACTAATTATACGGCTCTCATCTCATGCGCCATCGCGCTCCTTTTTGTGGGGTTTGTGAGCTCCAACGTAGACCAGGACAGGGCAGAGTGCGCGGACCAGGTGGTTGGGCTGGCCACTTGCTTGCCGTACGTGGGCGGGGAGGCGAGAGCACCGACGCCGGACTGTTGCAGTGGGCTCAAGGTTGTGCTTGAGAAGAGTAAGAAATGTCTTTGTGTGTTGATAAAGGACCGTAATGATCCAAGCCTGGGACTTAAAGTTAATCTTACGCTTGCTCTTGGACTGCCAAATGCATGCCATGCCCCTGCCAACATCCAAGACTGCATCGGGCTTCTCCATCTGTCGCCCAACTCCCAAGATGCTAAGGATTTTATGGGCTCCAAGGGAGCTAACACTACAGCCGCACCTGCTACTGCTGCGCCTGGGAATTCCACCCAAACTCCCATGGAGAAAGGTAGCGGAATGAGGAATCAATGGTTGGGTGCGGAGATGTTCATTGCGTTTCTCACTTCATCGCTTCTATTCACTTGTGTTTGGTAGTTCTATTTTAACCTTTTGTTCTTCCCTAATGTCCCAAAAATGTGAACCATCATATATGTGCTAAACTAATTGAAGTATGATTAAATGGTTAAAAGTTTTATTCTCATGGAGTTACAAATGTATATTTATATAAGGAACTGGCCTAGGATTTATCATCTCTCCTTCATAAATTAAGTTGAAAAACAACTAAATCGGACTCACTAAGGGTCTCCTAGGTTGTAGGCGTGCATAACGCACAACCCATTTGACAAACAGCCGTAATTCCTTTCTTCTCACAACCGACTATCTACTGTCTCGTCTCAAAGTTCGAGTGTTTTGCCTTCATCTTCATCTTATTTTACCCCCCTCCTTCTAAACATCTAAAAT

Coding sequence (CDS)

ATGGCCTCCAAATCCTCTTCAACTAATTATACGGCTCTCATCTCATGCGCCATCGCGCTCCTTTTTGTGGGGTTTGTGAGCTCCAACGTAGACCAGGACAGGGCAGAGTGCGCGGACCAGGTGGTTGGGCTGGCCACTTGCTTGCCGTACGTGGGCGGGGAGGCGAGAGCACCGACGCCGGACTGTTGCAGTGGGCTCAAGGTTGTGCTTGAGAAGAGTAAGAAATGTCTTTGTGTGTTGATAAAGGACCGTAATGATCCAAGCCTGGGACTTAAAGTTAATCTTACGCTTGCTCTTGGACTGCCAAATGCATGCCATGCCCCTGCCAACATCCAAGACTGCATCGGGCTTCTCCATCTGTCGCCCAACTCCCAAGATGCTAAGGATTTTATGGGCTCCAAGGGAGCTAACACTACAGCCGCACCTGCTACTGCTGCGCCTGGGAATTCCACCCAAACTCCCATGGAGAAAGGTAGCGGAATGAGGAATCAATGGTTGGGTGCGGAGATGTTCATTGCGTTTCTCACTTCATCGCTTCTATTCACTTGTGTTTGGTAG

Protein sequence

MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLLFTCVW
Homology
BLAST of CmaCh20G000380 vs. ExPASy Swiss-Prot
Match: O64865 (Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3702 GN=LTPG14 PE=2 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 9.9e-39
Identity = 91/197 (46.19%), Postives = 121/197 (61.42%), Query Frame = 0

Query: 13  LISCAIALLFVGFVSSNVD---QDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVV 72
           L++ AIAL+ V  V +  D   +D+ EC +Q+VG+ATCLPYV G+A++PTPDCCSGLK V
Sbjct: 8   LMATAIALIVVAMVVAAADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQV 67

Query: 73  LEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKD 132
           L  +KKCLCV+I+DRNDP LGL++N++LAL LP+ CHA A++  C  LLHL PNS DA+ 
Sbjct: 68  LNSNKKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQV 127

Query: 133 FMG-SKGANTT---AAPATAAPG--------------NSTQTPMEKG----SGMRNQWLG 185
           F   +KG N T   +AP  ++PG              NS +T    G         QWLG
Sbjct: 128 FYQLAKGLNKTGPASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRNHAQSFYKQWLG 187

BLAST of CmaCh20G000380 vs. ExPASy Swiss-Prot
Match: F4I082 (Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=3702 GN=LTPG6 PE=2 SV=2)

HSP 1 Score: 151.8 bits (382), Expect = 7.8e-36
Identity = 77/178 (43.26%), Postives = 110/178 (61.80%), Query Frame = 0

Query: 11  TALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVL 70
           T  I+  I  + +GF +S++ QDR EC +Q++ L+TC+PYVGG+A+APT DCC+G   V+
Sbjct: 7   TLFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVI 66

Query: 71  EKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPA-NIQDCIGLLHLSPNSQDAKD 130
            KS+KC+C+L++D++DP LG+K+N TLA  LP+ACH  A NI DCI +LHL  NS  AK+
Sbjct: 67  RKSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKE 126

Query: 131 F--MGSKGANTTAAPATA----APGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLLF 182
           F  +G    N  +   T       G     P++        WLG E+ I  L S + F
Sbjct: 127 FENLGRIEDNYNSTSPTQIHKDGTGGGKAEPVKSNGWKEKSWLGVELLIYLLVSLIFF 184

BLAST of CmaCh20G000380 vs. ExPASy Swiss-Prot
Match: O64864 (Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3702 GN=LTPG13 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.3e-35
Identity = 85/198 (42.93%), Postives = 117/198 (59.09%), Query Frame = 0

Query: 13  LISCAIALLFVGFV----SSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKV 72
           +++ AIAL+ V  V     ++  +D+ EC  Q+VG+ATCLPYV G+A++PTPDCCSGLK 
Sbjct: 8   VMATAIALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQ 67

Query: 73  VLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAK 132
           V+    KCLC++I++RNDP LGL+VN++LAL LP+ CHA A+I  C  LLHL PNS DA+
Sbjct: 68  VINSDMKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDPNSPDAQ 127

Query: 133 DFMG-SKGANTT-----------------AAPATAAPGNSTQTPMEKGS----GMRNQWL 185
            F   +KG N T                 + P ++A  NS +T    G+        QWL
Sbjct: 128 VFYQLAKGLNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQSFSKQWL 187

BLAST of CmaCh20G000380 vs. ExPASy Swiss-Prot
Match: Q9M2G1 (Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3702 GN=LTPG22 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 6.2e-25
Identity = 56/115 (48.70%), Postives = 78/115 (67.83%), Query Frame = 0

Query: 18  IALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEK--SKK 77
           +A++F+G V S + QD   C D +  L +CLP+V  +A+AP   CCS LKV ++K  ++K
Sbjct: 20  VAVMFLG-VRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKIDKGQTRK 79

Query: 78  CLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDF 131
           CLC L+KDR+DP LG KV+   A+ LP+ACH PANI  C  LLHL P+S  ++ F
Sbjct: 80  CLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDSPASQIF 133

BLAST of CmaCh20G000380 vs. ExPASy Swiss-Prot
Match: Q9LJ86 (Non-specific lipid transfer protein GPI-anchored 5 OS=Arabidopsis thaliana OX=3702 GN=LTPG5 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 8.7e-11
Identity = 45/145 (31.03%), Postives = 78/145 (53.79%), Query Frame = 0

Query: 21  LFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEKSKKCLCVL 80
           +F+  +SS +   ++ C + ++ ++ CL Y+ G + +P   CC+ L  V++ S  CLC +
Sbjct: 12  VFMAVMSSTMVSAQSSCTNALISMSPCLNYITGNSTSPNQQCCNQLSRVVQSSPDCLCQV 71

Query: 81  IKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDFMGSKGANTTA 140
           + +     LG+ VN T ALGLP AC    N+Q         P S+   +  G  G +T+ 
Sbjct: 72  L-NGGGSQLGINVNQTQALGLPRAC----NVQ-------TPPVSR--CNTGGGGGGSTSD 131

Query: 141 APA----TAAPGNSTQT-PMEKGSG 161
           +PA    ++ PGN ++T P+ +G G
Sbjct: 132 SPAESPNSSGPGNGSKTVPVGEGDG 142

BLAST of CmaCh20G000380 vs. ExPASy TrEMBL
Match: A0A6J1JBX4 (protein YLS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.4e-99
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0

Query: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60
           MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP
Sbjct: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60

Query: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120
           DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL
Sbjct: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120

Query: 121 SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL 180
           SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL
Sbjct: 121 SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL 180

Query: 181 FTCVW 186
           FTCVW
Sbjct: 181 FTCVW 185

BLAST of CmaCh20G000380 vs. ExPASy TrEMBL
Match: A0A6J1J894 (protein YLS3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.7e-97
Identity = 183/185 (98.92%), Postives = 183/185 (98.92%), Query Frame = 0

Query: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60
           MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP
Sbjct: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60

Query: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120
           DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL
Sbjct: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120

Query: 121 SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL 180
           SPNSQDAKDFMGSKGANTTAAP  AAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL
Sbjct: 121 SPNSQDAKDFMGSKGANTTAAP--AAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL 180

Query: 181 FTCVW 186
           FTCVW
Sbjct: 181 FTCVW 183

BLAST of CmaCh20G000380 vs. ExPASy TrEMBL
Match: A0A6J1FVR4 (protein YLS3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448546 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 5.9e-95
Identity = 176/185 (95.14%), Postives = 179/185 (96.76%), Query Frame = 0

Query: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60
           MASKSSSTNYTALI CA+ALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP
Sbjct: 1   MASKSSSTNYTALIPCAMALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60

Query: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120
           DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL
Sbjct: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120

Query: 121 SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL 180
           SPNSQDAKDFMGSKGANTTAAPA AAPGNSTQ+PMEKGSGMRNQW   EMFI FLTSSLL
Sbjct: 121 SPNSQDAKDFMGSKGANTTAAPAFAAPGNSTQSPMEKGSGMRNQWSSVEMFIGFLTSSLL 180

Query: 181 FTCVW 186
           FTC+W
Sbjct: 181 FTCIW 185

BLAST of CmaCh20G000380 vs. ExPASy TrEMBL
Match: A0A6J1FYE4 (protein YLS3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448546 PE=4 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 2.5e-93
Identity = 175/185 (94.59%), Postives = 178/185 (96.22%), Query Frame = 0

Query: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60
           MASKSSSTNYTALI CA+ALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP
Sbjct: 1   MASKSSSTNYTALIPCAMALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60

Query: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120
           DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL
Sbjct: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120

Query: 121 SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL 180
           SPNSQDAKDFMGSKGANTTAAP  AAPGNSTQ+PMEKGSGMRNQW   EMFI FLTSSLL
Sbjct: 121 SPNSQDAKDFMGSKGANTTAAP--AAPGNSTQSPMEKGSGMRNQWSSVEMFIGFLTSSLL 180

Query: 181 FTCVW 186
           FTC+W
Sbjct: 181 FTCIW 183

BLAST of CmaCh20G000380 vs. ExPASy TrEMBL
Match: A0A0A0KZ48 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280560 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 4.0e-67
Identity = 133/174 (76.44%), Postives = 150/174 (86.21%), Query Frame = 0

Query: 17  AIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEKSKKC 76
           A+ALL VGFVSSN+DQDRAECADQVVGLATCLPYVGGEA+APTPDCCSGLK+VL+KS+KC
Sbjct: 12  ALALLSVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLDKSRKC 71

Query: 77  LCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDFMG---S 136
           LCVLIKDR+DPSLGLKVNL+LALGLP+ACHAPANI+DC+GLLHLSPNS +AKDF+G   S
Sbjct: 72  LCVLIKDRDDPSLGLKVNLSLALGLPSACHAPANIKDCVGLLHLSPNSPEAKDFLGSPNS 131

Query: 137 KGANT-TAAPATAAPGNSTQTPMEKGSG-MRNQWLGAEMFIAFLTSSLLFTCVW 186
           K  NT T A AT   G+S+Q    K  G  RNQWLG EMFI F+TSS LFTC+W
Sbjct: 132 KETNTSTPAHATPVSGSSSQNSEVKNDGTKRNQWLGVEMFIWFITSSFLFTCIW 185

BLAST of CmaCh20G000380 vs. NCBI nr
Match: XP_022986686.1 (protein YLS3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 372.1 bits (954), Expect = 2.8e-99
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0

Query: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60
           MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP
Sbjct: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60

Query: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120
           DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL
Sbjct: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120

Query: 121 SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL 180
           SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL
Sbjct: 121 SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL 180

Query: 181 FTCVW 186
           FTCVW
Sbjct: 181 FTCVW 185

BLAST of CmaCh20G000380 vs. NCBI nr
Match: XP_022986687.1 (protein YLS3-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 364.0 bits (933), Expect = 7.7e-97
Identity = 183/185 (98.92%), Postives = 183/185 (98.92%), Query Frame = 0

Query: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60
           MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP
Sbjct: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60

Query: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120
           DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL
Sbjct: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120

Query: 121 SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL 180
           SPNSQDAKDFMGSKGANTTAAP  AAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL
Sbjct: 121 SPNSQDAKDFMGSKGANTTAAP--AAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL 180

Query: 181 FTCVW 186
           FTCVW
Sbjct: 181 FTCVW 183

BLAST of CmaCh20G000380 vs. NCBI nr
Match: XP_023512874.1 (protein YLS3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 362.5 bits (929), Expect = 2.2e-96
Identity = 178/185 (96.22%), Postives = 182/185 (98.38%), Query Frame = 0

Query: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60
           MASKSSSTNYTALISCA+ALL VGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP
Sbjct: 1   MASKSSSTNYTALISCAMALLLVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60

Query: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120
           DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL
Sbjct: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120

Query: 121 SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL 180
           SPNSQDAKDFMG+KGANTTAAPATAAPGNSTQTP+EKGSGMRNQWLG EMFI FLTSSLL
Sbjct: 121 SPNSQDAKDFMGTKGANTTAAPATAAPGNSTQTPVEKGSGMRNQWLGVEMFIGFLTSSLL 180

Query: 181 FTCVW 186
           FTC+W
Sbjct: 181 FTCIW 185

BLAST of CmaCh20G000380 vs. NCBI nr
Match: KAG7010231.1 (Protein YLS3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 358.6 bits (919), Expect = 3.2e-95
Identity = 176/185 (95.14%), Postives = 181/185 (97.84%), Query Frame = 0

Query: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60
           MASKSSSTNYTAL+SCA+ALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP
Sbjct: 1   MASKSSSTNYTALMSCAMALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60

Query: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120
           DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL
Sbjct: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120

Query: 121 SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL 180
           SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQ+P+EKGSGMRNQW   EMFI FLTSSLL
Sbjct: 121 SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQSPIEKGSGMRNQWSSVEMFIGFLTSSLL 180

Query: 181 FTCVW 186
           FTC+W
Sbjct: 181 FTCIW 185

BLAST of CmaCh20G000380 vs. NCBI nr
Match: XP_022943979.1 (protein YLS3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 356.7 bits (914), Expect = 1.2e-94
Identity = 176/185 (95.14%), Postives = 179/185 (96.76%), Query Frame = 0

Query: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60
           MASKSSSTNYTALI CA+ALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP
Sbjct: 1   MASKSSSTNYTALIPCAMALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60

Query: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120
           DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL
Sbjct: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120

Query: 121 SPNSQDAKDFMGSKGANTTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLL 180
           SPNSQDAKDFMGSKGANTTAAPA AAPGNSTQ+PMEKGSGMRNQW   EMFI FLTSSLL
Sbjct: 121 SPNSQDAKDFMGSKGANTTAAPAFAAPGNSTQSPMEKGSGMRNQWSSVEMFIGFLTSSLL 180

Query: 181 FTCVW 186
           FTC+W
Sbjct: 181 FTCIW 185

BLAST of CmaCh20G000380 vs. TAIR 10
Match: AT2G44300.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 161.4 bits (407), Expect = 7.0e-40
Identity = 91/197 (46.19%), Postives = 121/197 (61.42%), Query Frame = 0

Query: 13  LISCAIALLFVGFVSSNVD---QDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVV 72
           L++ AIAL+ V  V +  D   +D+ EC +Q+VG+ATCLPYV G+A++PTPDCCSGLK V
Sbjct: 8   LMATAIALIVVAMVVAAADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQV 67

Query: 73  LEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKD 132
           L  +KKCLCV+I+DRNDP LGL++N++LAL LP+ CHA A++  C  LLHL PNS DA+ 
Sbjct: 68  LNSNKKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQV 127

Query: 133 FMG-SKGANTT---AAPATAAPG--------------NSTQTPMEKG----SGMRNQWLG 185
           F   +KG N T   +AP  ++PG              NS +T    G         QWLG
Sbjct: 128 FYQLAKGLNKTGPASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRNHAQSFYKQWLG 187

BLAST of CmaCh20G000380 vs. TAIR 10
Match: AT1G55260.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 154.5 bits (389), Expect = 8.6e-38
Identity = 77/175 (44.00%), Postives = 111/175 (63.43%), Query Frame = 0

Query: 11  TALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVL 70
           T  I+  I  + +GF +S++ QDR EC +Q++ L+TC+PYVGG+A+APT DCC+G   V+
Sbjct: 50  TLFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVI 109

Query: 71  EKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPA-NIQDCIGLLHLSPNSQDAKD 130
            KS+KC+C+L++D++DP LG+K+N TLA  LP+ACH  A NI DCI +LHL  NS  AK+
Sbjct: 110 RKSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKE 169

Query: 131 F--MGSKGAN-TTAAPATAAPGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLLF 182
           F  +G    N  + +P     G     P++        WLG E+ I  L S + F
Sbjct: 170 FENLGRIEDNYNSTSPTQIHKGGGKAEPVKSNGWKEKSWLGVELLIYLLVSLIFF 224

BLAST of CmaCh20G000380 vs. TAIR 10
Match: AT1G55260.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 151.8 bits (382), Expect = 5.6e-37
Identity = 77/178 (43.26%), Postives = 110/178 (61.80%), Query Frame = 0

Query: 11  TALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVL 70
           T  I+  I  + +GF +S++ QDR EC +Q++ L+TC+PYVGG+A+APT DCC+G   V+
Sbjct: 50  TLFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVI 109

Query: 71  EKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPA-NIQDCIGLLHLSPNSQDAKD 130
            KS+KC+C+L++D++DP LG+K+N TLA  LP+ACH  A NI DCI +LHL  NS  AK+
Sbjct: 110 RKSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKE 169

Query: 131 F--MGSKGANTTAAPATA----APGNSTQTPMEKGSGMRNQWLGAEMFIAFLTSSLLF 182
           F  +G    N  +   T       G     P++        WLG E+ I  L S + F
Sbjct: 170 FENLGRIEDNYNSTSPTQIHKDGTGGGKAEPVKSNGWKEKSWLGVELLIYLLVSLIFF 227

BLAST of CmaCh20G000380 vs. TAIR 10
Match: AT2G44290.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 151.0 bits (380), Expect = 9.5e-37
Identity = 85/198 (42.93%), Postives = 117/198 (59.09%), Query Frame = 0

Query: 13  LISCAIALLFVGFV----SSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKV 72
           +++ AIAL+ V  V     ++  +D+ EC  Q+VG+ATCLPYV G+A++PTPDCCSGLK 
Sbjct: 8   VMATAIALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQ 67

Query: 73  VLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAK 132
           V+    KCLC++I++RNDP LGL+VN++LAL LP+ CHA A+I  C  LLHL PNS DA+
Sbjct: 68  VINSDMKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDPNSPDAQ 127

Query: 133 DFMG-SKGANTT-----------------AAPATAAPGNSTQTPMEKGS----GMRNQWL 185
            F   +KG N T                 + P ++A  NS +T    G+        QWL
Sbjct: 128 VFYQLAKGLNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQSFSKQWL 187

BLAST of CmaCh20G000380 vs. TAIR 10
Match: AT3G58550.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 115.5 bits (288), Expect = 4.4e-26
Identity = 56/115 (48.70%), Postives = 78/115 (67.83%), Query Frame = 0

Query: 18  IALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEK--SKK 77
           +A++F+G V S + QD   C D +  L +CLP+V  +A+AP   CCS LKV ++K  ++K
Sbjct: 20  VAVMFLG-VRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKIDKGQTRK 79

Query: 78  CLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDF 131
           CLC L+KDR+DP LG KV+   A+ LP+ACH PANI  C  LLHL P+S  ++ F
Sbjct: 80  CLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDSPASQIF 133

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O648659.9e-3946.19Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3... [more]
F4I0827.8e-3643.26Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=37... [more]
O648641.3e-3542.93Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3... [more]
Q9M2G16.2e-2548.70Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3... [more]
Q9LJ868.7e-1131.03Non-specific lipid transfer protein GPI-anchored 5 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A6J1JBX41.4e-99100.00protein YLS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV... [more]
A0A6J1J8943.7e-9798.92protein YLS3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV... [more]
A0A6J1FVR45.9e-9595.14protein YLS3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448546 PE=4 ... [more]
A0A6J1FYE42.5e-9394.59protein YLS3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448546 PE=4 ... [more]
A0A0A0KZ484.0e-6776.44AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280560 PE=4 SV... [more]
Match NameE-valueIdentityDescription
XP_022986686.12.8e-99100.00protein YLS3-like isoform X1 [Cucurbita maxima][more]
XP_022986687.17.7e-9798.92protein YLS3-like isoform X2 [Cucurbita maxima][more]
XP_023512874.12.2e-9696.22protein YLS3-like [Cucurbita pepo subsp. pepo][more]
KAG7010231.13.2e-9595.14Protein YLS3 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022943979.11.2e-9495.14protein YLS3-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G44300.17.0e-4046.19Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT1G55260.28.6e-3844.00Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT1G55260.15.6e-3743.26Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT2G44290.19.5e-3742.93Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT3G58550.14.4e-2648.70Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainSMARTSM00499aai_6coord: 37..114
e-value: 2.9E-5
score: 33.5
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainPFAMPF14368LTP_2coord: 28..114
e-value: 7.4E-12
score: 45.2
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilyGENE3D1.10.110.10coord: 34..113
e-value: 9.3E-14
score: 53.1
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilySUPERFAMILY47699Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumincoord: 36..113
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 129..159
NoneNo IPR availablePANTHERPTHR33044:SF194BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEINcoord: 11..176
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..16
score: 5.0
NoneNo IPR availableCDDcd00010AAI_LTSScoord: 44..106
e-value: 2.08985E-16
score: 68.2222
IPR043325Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) ProteinPANTHERPTHR33044BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATEDcoord: 11..176

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G000380.1CmaCh20G000380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane