CmaCh20G000050 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh20G000050
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	RING-type E3 ubiquitin transferase
Location	Cma_Chr20: 13841 .. 14968 (+)
RNA-Seq Expression	CmaCh20G000050
Synteny	CmaCh20G000050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACGAGTCAATTGCAACAAGATATTGGTGTCACGTGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCTTTCTGCCGTAGTGGCTTTGTTGAAGAGATGGGTAACGGTATGTCCAGCGACAATAATGGTAATAATAATAATAATAGTAATAATGCGCTTGAATCCGATTTTGGGTCGGAGCGAGCTTTGTCCCTCTGGGCCCCGATACTGCTGGGTATGATGGGAAATCCCCGTGGGCGTCGTAGATTCAGGCATCTAGAGTTTGACGAAGATGATGATAATAATAATGAGCAGGAGCATGGGGATGGGGAGGGGAACCGTGCGAGTAGTGAGCCGGCTGAGCTTGATTCGGTCATTAGGAGGAGGAGGAGAAGTTCGGCTACCATTCTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGGTGAATCAGAGAATAGTTACGAGGGAGAGAGGAGCAGAGAAAGAGAAAGAGAAAGAGAACGAGAACGAGAAAGAGAGCGGGTTATCCTTATAAATCCTTTCAACCAGACCATTGTAGTTCAAGGAGGTGAGAGTCAAAATCATAACGCTATAGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATTTAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCCCAGAAGGAGGCAGTTGATGCGCTGCCCACGGTGAGAGTTGAATGCGGGGATAATCGTCTGCAATGTTCGGTGTGTTTGGATGAGTTTGAGATGGATGAAGAAGCCAAGGAGATGCCTTGTAAGCATAAGTTTCATTCAGGGTGTATTCTGCCGTGGCTGGAGCTGCATAGTTCCTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAGTCCAAGCGTGATACGGATGGTGGGTCGGCTAGGTTTATGGGTGAGCTTAGTAATGGTAATGATAGTGGCAGTGGCAGTGGCAGTGGGGAGATTGATGGGAGAACCGCATCTGGGATTGGGAGAAGGTTTTCGTTTCCATGGCCATTCAATGGCTTGTTTTCTTCGCAAGGAAACTCCTCGGGAGGAAGTGAAAGTGAGTCTCAAAGGGATCACACACCCATTTGA

mRNA sequence

ATGGACGAGTCAATTGCAACAAGATATTGGTGTCACGTGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCTTTCTGCCGTAGTGGCTTTGTTGAAGAGATGGGTAACGGTATGTCCAGCGACAATAATGGTAATAATAATAATAATAGTAATAATGCGCTTGAATCCGATTTTGGGTCGGAGCGAGCTTTGTCCCTCTGGGCCCCGATACTGCTGGGTATGATGGGAAATCCCCGTGGGCGTCGTAGATTCAGGCATCTAGAGTTTGACGAAGATGATGATAATAATAATGAGCAGGAGCATGGGGATGGGGAGGGGAACCGTGCGAGTAGTGAGCCGGCTGAGCTTGATTCGGTCATTAGGAGGAGGAGGAGAAGTTCGGCTACCATTCTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGGTGAATCAGAGAATAGTTACGAGGGAGAGAGGAGCAGAGAAAGAGAAAGAGAAAGAGAACGAGAACGAGAAAGAGAGCGGGTTATCCTTATAAATCCTTTCAACCAGACCATTGTAGTTCAAGGAGGTGAGAGTCAAAATCATAACGCTATAGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATTTAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCCCAGAAGGAGGCAGTTGATGCGCTGCCCACGGTGAGAGTTGAATGCGGGGATAATCGTCTGCAATGTTCGGTGTGTTTGGATGAGTTTGAGATGGATGAAGAAGCCAAGGAGATGCCTTGTAAGCATAAGTTTCATTCAGGGTGTATTCTGCCGTGGCTGGAGCTGCATAGTTCCTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAGTCCAAGCGTGATACGGATGGTGGGTCGGCTAGGTTTATGGGTGAGCTTAGTAATGGTAATGATAGTGGCAGTGGCAGTGGCAGTGGGGAGATTGATGGGAGAACCGCATCTGGGATTGGGAGAAGGTTTTCGTTTCCATGGCCATTCAATGGCTTGTTTTCTTCGCAAGGAAACTCCTCGGGAGGAAGTGAAAGTGAGTCTCAAAGGGATCACACACCCATTTGA

Coding sequence (CDS)

ATGGACGAGTCAATTGCAACAAGATATTGGTGTCACGTGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCTTTCTGCCGTAGTGGCTTTGTTGAAGAGATGGGTAACGGTATGTCCAGCGACAATAATGGTAATAATAATAATAATAGTAATAATGCGCTTGAATCCGATTTTGGGTCGGAGCGAGCTTTGTCCCTCTGGGCCCCGATACTGCTGGGTATGATGGGAAATCCCCGTGGGCGTCGTAGATTCAGGCATCTAGAGTTTGACGAAGATGATGATAATAATAATGAGCAGGAGCATGGGGATGGGGAGGGGAACCGTGCGAGTAGTGAGCCGGCTGAGCTTGATTCGGTCATTAGGAGGAGGAGGAGAAGTTCGGCTACCATTCTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGGTGAATCAGAGAATAGTTACGAGGGAGAGAGGAGCAGAGAAAGAGAAAGAGAAAGAGAACGAGAACGAGAAAGAGAGCGGGTTATCCTTATAAATCCTTTCAACCAGACCATTGTAGTTCAAGGAGGTGAGAGTCAAAATCATAACGCTATAGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATTTAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCCCAGAAGGAGGCAGTTGATGCGCTGCCCACGGTGAGAGTTGAATGCGGGGATAATCGTCTGCAATGTTCGGTGTGTTTGGATGAGTTTGAGATGGATGAAGAAGCCAAGGAGATGCCTTGTAAGCATAAGTTTCATTCAGGGTGTATTCTGCCGTGGCTGGAGCTGCATAGTTCCTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAGTCCAAGCGTGATACGGATGGTGGGTCGGCTAGGTTTATGGGTGAGCTTAGTAATGGTAATGATAGTGGCAGTGGCAGTGGCAGTGGGGAGATTGATGGGAGAACCGCATCTGGGATTGGGAGAAGGTTTTCGTTTCCATGGCCATTCAATGGCTTGTTTTCTTCGCAAGGAAACTCCTCGGGAGGAAGTGAAAGTGAGTCTCAAAGGGATCACACACCCATTTGA

Protein sequence

MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILINPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNSSGGSESESQRDHTPI

Homology

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BLAST of CmaCh20G000050 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 5.1e-50
Identity = 141/385 (36.62%), Postives = 194/385 (50.39%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           M E+  TRYWCH C Q +   +  EIKCP C  GFVEEM     +D       N      
Sbjct: 1   MAEAAITRYWCHECEQAIEEAMVDEIKCPSCGGGFVEEM-----TDEEIERLTNRQ---- 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120
                E   S W PI                   D DD++N+                 E
Sbjct: 61  ----PEPGFSQWNPI------------EHPGETMDSDDEDNDLGR--------------E 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180
            +  IRR RR+S T+ ++L  I   +A                      ++ER+  ILIN
Sbjct: 121 FEGFIRRHRRAS-TLRRVLDSIHDDLAD---------------------DQERDSSILIN 180

Query: 181 PFNQTIVVQGG----------ESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPP 240
            FNQ + +QG           +  + N  G L +Y +G GL LLLQHLAE+DP+R GTPP
Sbjct: 181 AFNQALALQGSVLDPDEGQGDQGGSTNDDGLLEEYVLGAGLSLLLQHLAESDPSRNGTPP 240

Query: 241 AQKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSC 300
           A+KEAV+ALPTV++E     + CSVCLD+ E+  +AK+MPC+HKFHS CILPWLELHSSC
Sbjct: 241 AKKEAVEALPTVKIE---EVVSCSVCLDDLEVGSQAKQMPCEHKFHSSCILPWLELHSSC 300

Query: 301 PVCRHQLPADESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSF-PWP 360
           PVCR +LP++E+K   D      +G + + ++     G G +    +    R ++  PW 
Sbjct: 301 PVCRFELPSEETK---DLNEPSNIGRVEDSHEEVRADGPGNV----SESSNRPWAIVPW- 313

Query: 361 FNGLFSS-QGNSSGGSESESQRDHT 374
            N LFS+ +  ++GG  ++ Q  HT
Sbjct: 361 LNELFSTREAQNAGGVSTDQQSPHT 313

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BLAST of CmaCh20G000050 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.4e-36
Identity = 100/251 (39.84%), Postives = 133/251 (52.99%), Query Frame = 0


Query: 133 ATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILINPFN------QTI 192
           +T+L L+ G  A   +G    S  G   + + R  ++  + +     +PF       QT+
Sbjct: 80  STLLPLIFGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQSD---AFDPFTFLQNHLQTL 139

Query: 193 ---------VVQGGESQNHNAI-GSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAV 252
                    V++   S   N + G+ GDYF GPGL+ L+Q LAENDPNRYGTPPA K A+
Sbjct: 140 RSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAI 199

Query: 253 DALPTVRVE---CGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVC 312
           DALPTV+V          QC+VC+DEFE   + K+MPCKH FH  C+LPWLELH+SCPVC
Sbjct: 200 DALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVC 259

Query: 313 RHQLPADESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFS--FPWPFN 363
           R +LP D+              E  +    GSG G G ++G+       RFS   PWPF 
Sbjct: 260 RFELPTDDPDY-----------ENRSQGSQGSGDGQGSVEGQQTP----RFSIQLPWPFR 309

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BLAST of CmaCh20G000050 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.6e-35
Identity = 121/372 (32.53%), Postives = 161/372 (43.28%), Query Frame = 0


Query: 9   YWCHVCSQMVNPIV---DVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALESDFG- 68
           ++C+ C++ VN  +     +  CP C  GF+EE  N   +  +G  N N N+    D   
Sbjct: 23  FFCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFL 82

Query: 69  --SERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAELD 128
             S+   SL  P+L     +                D NN    G     R   +P   D
Sbjct: 83  TLSDPFASL-LPLLFPSSSSTT-------TSSSASIDPNNPSLSGPTRSGR--GDPFAFD 142

Query: 129 SVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILINPF 188
                       I   L  +R+  A                        + E VI  NP 
Sbjct: 143 PF--------TFIQNHLNDLRSSGA------------------------QIEFVIQNNP- 202

Query: 189 NQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTV 248
                      Q      ++GDYF+GPGL+ L+Q LAENDPNRYGTPPA K A++ALP V
Sbjct: 203 ---------SDQGFRLPANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLV 262

Query: 249 RV---ECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPA 308
            +          QC+VC+D+FE   EAK+MPCKH +H  C+LPWLELH+SCPVCRH+LP 
Sbjct: 263 NITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPT 322

Query: 309 DESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGN 368
           D+   +      R     S GND  +     + D RT      R S PWPF       G 
Sbjct: 323 DDPDYER---RVRGAQGTSGGNDGDNSGQRSDGDNRTVER-SFRISLPWPFQA--RGPGP 336

Query: 369 SSGGSESESQRD 372
           + G +    Q D
Sbjct: 383 APGDNAETRQED 336

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BLAST of CmaCh20G000050 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 5.1e-26
Identity = 68/177 (38.42%), Postives = 94/177 (53.11%), Query Frame = 0


Query: 200 GSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRV---ECGDNRLQCSVC 259
           G+ GDYF GPGL+ L + L+     R G PPA + A+DALPT+++       +   C VC
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTTRR-GPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 260 LDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADESKRDTDGGSARFMGE 319
            DEFE+  EAK+MPC H +HS CI+PWL  H+SCPVCR +LP+      +   +      
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

Query: 320 LSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPF--NGLFSSQGNSSGGSESESQRD 372
            S+ + S S S     + R        FS  WPF  +G  SS   + GG+ +    D
Sbjct: 255 RSSSSSSSSNSRENGNERRNP------FSSFWPFRSSGSSSSSTQNRGGTRNSDTSD 304

[Close]

BLAST of CmaCh20G000050 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.1e-23
Identity = 82/250 (32.80%), Postives = 116/250 (46.40%), Query Frame = 0


Query: 138 LLQGIRAGMATGESENSYEGERSRERERERERERERERVILINPFNQTIVVQGGESQNHN 197
           L++   A M +  +E SY+ E S                +LI        + GG++ +  
Sbjct: 56  LMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVE 115

Query: 198 A--------IG-----SLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRV 257
           A        IG     + GDYF GPGL+ L++ L+    +R G PPA K ++DALPT+++
Sbjct: 116 AFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHR-GPPPAPKSSIDALPTIKI 175

Query: 258 ---ECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADE 317
                  +   C VC DEFE+  EAK+MPC H +HS CI+PWL  H+SCPVCR +LP+  
Sbjct: 176 TQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRG 235

Query: 318 SKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNSS 372
           S   T     R                    +GR  S     FS  WPF         SS
Sbjct: 236 SSSSTQSSQNR------------------STNGRENSRRRNIFSNLWPF--------RSS 278

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BLAST of CmaCh20G000050 vs. ExPASy TrEMBL
Match: A0A6J1JDY5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 PE=4 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 1.7e-213
Identity = 375/375 (100.00%), Postives = 375/375 (100.00%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120
           SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE
Sbjct: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180
           LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN
Sbjct: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180

Query: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240
           PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP
Sbjct: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240

Query: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300
           TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD
Sbjct: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300

Query: 301 ESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS 360
           ESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS
Sbjct: 301 ESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS 360

Query: 361 SGGSESESQRDHTPI 376
           SGGSESESQRDHTPI
Sbjct: 361 SGGSESESQRDHTPI 375

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BLAST of CmaCh20G000050 vs. ExPASy TrEMBL
Match: A0A6J1JBZ6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 PE=4 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 2.2e-205
Identity = 364/375 (97.07%), Postives = 364/375 (97.07%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120
           SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE
Sbjct: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180
           LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN
Sbjct: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180

Query: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240
           PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP
Sbjct: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240

Query: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300
           TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD
Sbjct: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300

Query: 301 ESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS 360
           ESKRDTDGGSARFM           GSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS
Sbjct: 301 ESKRDTDGGSARFM-----------GSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS 360

Query: 361 SGGSESESQRDHTPI 376
           SGGSESESQRDHTPI
Sbjct: 361 SGGSESESQRDHTPI 364

[Close]

BLAST of CmaCh20G000050 vs. ExPASy TrEMBL
Match: A0A6J1FWF9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781 PE=4 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 7.5e-198
Identity = 355/376 (94.41%), Postives = 360/376 (95.74%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG-NNNNNSNNAL 60
           MDESIATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG NNNNNSNNAL
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPA 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDD+NN+QEHGDGEGNR SSEP 
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILI 180
           ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS  RERERERERERERVILI
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS--RERERERERERERVILI 180

Query: 181 NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240
           NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL
Sbjct: 181 NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPA 300
           PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPC+HKFHSGCILPWLELHSSCPVCRHQLPA
Sbjct: 241 PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPA 300

Query: 301 DESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGN 360
           DESKRD DGGSARFM     G+ SGSGSGSGEIDGR ASG GRRFSFPWPFNGLFSSQGN
Sbjct: 301 DESKRDADGGSARFM-----GSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGN 360

Query: 361 SSGGSESESQRDHTPI 376
           SSGGS SESQRDHT I
Sbjct: 361 SSGGSGSESQRDHTSI 369

[Close]

BLAST of CmaCh20G000050 vs. ExPASy TrEMBL
Match: A0A1S3BNR7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.7e-171
Identity = 317/380 (83.42%), Postives = 339/380 (89.21%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNN-GNNNNNSNNAL 60
           MDE IATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEE+GNG+SSDNN  NNNNN+NN L
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPA 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDD+N   EH DGEGN  SSE  
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNHVSSETT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILI 180
           ELDSVI RRRRSSATILQLLQGIRAG+AT ESENSYEGERS          RERERVILI
Sbjct: 121 ELDSVI-RRRRSSATILQLLQGIRAGIATAESENSYEGERS----------RERERVILI 180

Query: 181 NPFNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVD 240
           NPFNQTIVVQGGE  +QNHN+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVD
Sbjct: 181 NPFNQTIVVQGGENQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVD 240

Query: 241 ALPTVRVEC-GDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQ 300
           ALPTVRVE   D+RLQCSVCLDEFE+DEEAKEMPCKHKFH+GCILPWLELHSSCPVCRHQ
Sbjct: 241 ALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQ 300

Query: 301 LPADESKRDTDGGS-ARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFS 360
           LP DESKRD DGGS ARFM +L+NGN +G+G+GSGEI+ R +S  GRRFSFPWPFNGLF+
Sbjct: 301 LPGDESKRDVDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFT 360

Query: 361 SQGNSSGGSESESQRDHTPI 376
           SQGNSS GS S+SQRDHT I
Sbjct: 361 SQGNSSAGSASDSQRDHTSI 366

[Close]

BLAST of CmaCh20G000050 vs. ExPASy TrEMBL
Match: A0A5D3DDU3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001160 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.7e-171
Identity = 317/380 (83.42%), Postives = 339/380 (89.21%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNN-GNNNNNSNNAL 60
           MDE IATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEE+GNG+SSDNN  NNNNN+NN L
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPA 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDD+N   EH DGEGN  SSE  
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNHVSSETT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILI 180
           ELDSVI RRRRSSATILQLLQGIRAG+AT ESENSYEGERS          RERERVILI
Sbjct: 121 ELDSVI-RRRRSSATILQLLQGIRAGIATAESENSYEGERS----------RERERVILI 180

Query: 181 NPFNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVD 240
           NPFNQTIVVQGGE  +QNHN+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVD
Sbjct: 181 NPFNQTIVVQGGENQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVD 240

Query: 241 ALPTVRVEC-GDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQ 300
           ALPTVRVE   D+RLQCSVCLDEFE+DEEAKEMPCKHKFH+GCILPWLELHSSCPVCRHQ
Sbjct: 241 ALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQ 300

Query: 301 LPADESKRDTDGGS-ARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFS 360
           LP DESKRD DGGS ARFM +L+NGN +G+G+GSGEI+ R +S  GRRFSFPWPFNGLF+
Sbjct: 301 LPGDESKRDVDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFT 360

Query: 361 SQGNSSGGSESESQRDHTPI 376
           SQGNSS GS S+SQRDHT I
Sbjct: 361 SQGNSSAGSASDSQRDHTSI 366

[Close]

BLAST of CmaCh20G000050 vs. NCBI nr
Match: XP_022985674.1 (E3 ubiquitin-protein ligase SIRP1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 751.5 bits (1939), Expect = 3.5e-213
Identity = 375/375 (100.00%), Postives = 375/375 (100.00%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120
           SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE
Sbjct: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180
           LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN
Sbjct: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180

Query: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240
           PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP
Sbjct: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240

Query: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300
           TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD
Sbjct: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300

Query: 301 ESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS 360
           ESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS
Sbjct: 301 ESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS 360

Query: 361 SGGSESESQRDHTPI 376
           SGGSESESQRDHTPI
Sbjct: 361 SGGSESESQRDHTPI 375

[Close]

BLAST of CmaCh20G000050 vs. NCBI nr
Match: XP_022985675.1 (E3 ubiquitin-protein ligase SIRP1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 724.5 bits (1869), Expect = 4.5e-205
Identity = 364/375 (97.07%), Postives = 364/375 (97.07%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120
           SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE
Sbjct: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180
           LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN
Sbjct: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180

Query: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240
           PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP
Sbjct: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240

Query: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300
           TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD
Sbjct: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300

Query: 301 ESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS 360
           ESKRDTDGGSARFM           GSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS
Sbjct: 301 ESKRDTDGGSARFM-----------GSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS 360

Query: 361 SGGSESESQRDHTPI 376
           SGGSESESQRDHTPI
Sbjct: 361 SGGSESESQRDHTPI 364

[Close]

BLAST of CmaCh20G000050 vs. NCBI nr
Match: KAG7010206.1 (E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 708.0 bits (1826), Expect = 4.4e-200
Identity = 357/375 (95.20%), Postives = 360/375 (96.00%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           MDESIATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120
           SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDD+NN+QEHGDGEGNR SSEP E
Sbjct: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180
           LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS  RERERERERERERVILIN
Sbjct: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS--RERERERERERERVILIN 180

Query: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240
           PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP
Sbjct: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240

Query: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300
           TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD
Sbjct: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300

Query: 301 ESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNS 360
           ESKRD DGGSARFMG       SGSGSGSGEIDGR ASG GRRFSFPWPFNGLFSSQGNS
Sbjct: 301 ESKRDADGGSARFMG-------SGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNS 360

Query: 361 SGGSESESQRDHTPI 376
           SGGSESESQRDHT I
Sbjct: 361 SGGSESESQRDHTSI 366

[Close]

BLAST of CmaCh20G000050 vs. NCBI nr
Match: XP_022944289.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata])

HSP 1 Score: 699.5 bits (1804), Expect = 1.6e-197
Identity = 355/376 (94.41%), Postives = 360/376 (95.74%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG-NNNNNSNNAL 60
           MDESIATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG NNNNNSNNAL
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPA 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDD+NN+QEHGDGEGNR SSEP 
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILI 180
           ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS  RERERERERERERVILI
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS--RERERERERERERVILI 180

Query: 181 NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240
           NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL
Sbjct: 181 NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPA 300
           PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPC+HKFHSGCILPWLELHSSCPVCRHQLPA
Sbjct: 241 PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPA 300

Query: 301 DESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGN 360
           DESKRD DGGSARFM     G+ SGSGSGSGEIDGR ASG GRRFSFPWPFNGLFSSQGN
Sbjct: 301 DESKRDADGGSARFM-----GSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGN 360

Query: 361 SSGGSESESQRDHTPI 376
           SSGGS SESQRDHT I
Sbjct: 361 SSGGSGSESQRDHTSI 369

[Close]

BLAST of CmaCh20G000050 vs. NCBI nr
Match: XP_023512553.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 698.4 bits (1801), Expect = 3.5e-197
Identity = 352/377 (93.37%), Postives = 357/377 (94.69%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           MDESI TRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEEMGNG+SS+NNGNNNNNSNNALE
Sbjct: 1   MDESIPTRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGVSSENNGNNNNNSNNALE 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120
           SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDD+NN+QEHGDGEGNR SSEP E
Sbjct: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTE 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS--RERERERERERERERVIL 180
           LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS  RERERERERERERERVIL
Sbjct: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERERVIL 180

Query: 181 INPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDA 240
           INPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDA
Sbjct: 181 INPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDA 240

Query: 241 LPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLP 300
           LPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLP
Sbjct: 241 LPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLP 300

Query: 301 ADESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQG 360
           ADESKRD DGGSARFM           GSGSGEIDGR ASG GRRFSFPWPFNGLFSSQG
Sbjct: 301 ADESKRDADGGSARFM-----------GSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQG 360

Query: 361 NSSGGSESESQRDHTPI 376
           NSSGGSESESQRDHT I
Sbjct: 361 NSSGGSESESQRDHTSI 366

[Close]

BLAST of CmaCh20G000050 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 378.3 bits (970), Expect = 7.4e-105
Identity = 220/398 (55.28%), Postives = 274/398 (68.84%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEM-GNGMSSDNNGNNNNNSNNAL 60
           M+E++A RYWCH+CSQMVNP+++ EIKCPFC+SGF+EEM GNG      G  +   +   
Sbjct: 1   MEETMAARYWCHMCSQMVNPVMESEIKCPFCQSGFIEEMSGNGGGGGGRGIRDVQDS--- 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQ--EHGDGEGN----- 120
           E+DFG++RALSLWAPILLGMM +PR RRRFR  EF E++D+N +    + DG GN     
Sbjct: 61  ETDFGTDRALSLWAPILLGMMSSPRRRRRFRRSEFGEENDDNGDDLTTNADGNGNDSNNS 120

Query: 121 --------RASSEPAELD------SVIRRRRRSSATILQLLQGIRAGMATGESENSYEGE 180
                   RA     E+D      S++RRRRRSS  ILQLLQGIRAG+A+ E E+S    
Sbjct: 121 NNNVYRHHRARRHGGEIDLDREFESILRRRRRSSGNILQLLQGIRAGIAS-EYESS---- 180

Query: 181 RSRERERERERERERERVILINPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHL 240
                +   +  RER+RVI+INP+NQ++VV   ++QNH ++ SLGDYF+GPGLDLLLQHL
Sbjct: 181 -----DNNWDNSRERDRVIMINPYNQSLVVPSDQNQNHPSLTSLGDYFIGPGLDLLLQHL 240

Query: 241 AENDPNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSG 300
           AENDPNR GTPPA+KEAV+ALPTV++      LQCSVCLD+FE   EAKEMPCKHKFH  
Sbjct: 241 AENDPNRQGTPPARKEAVEALPTVKIM---EPLQCSVCLDDFEKGTEAKEMPCKHKFHVR 300

Query: 301 CILPWLELHSSCPVCRHQLPA-----DESKRDTDGG-SARFMGELSNGN-DSGSGSGSGE 360
           CI+PWLELHSSCPVCR +LP+     DE+K D++     R + E SNGN     G+    
Sbjct: 301 CIVPWLELHSSCPVCRFELPSSADDDDETKTDSERVLRTRNVRETSNGNVVENVGNADRG 360

Query: 361 IDGRTASGIGRRFSFPWPFNGLFSSQGNSSGGSESESQ 370
            +    SG GRRFSFPWPF+GLFSS  +SS  S S SQ
Sbjct: 361 REDEVRSGNGRRFSFPWPFSGLFSS-SSSSSSSTSGSQ 381

[Close]

BLAST of CmaCh20G000050 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 317.0 bits (811), Expect = 2.0e-86
Identity = 182/380 (47.89%), Postives = 242/380 (63.68%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNALE 60
           M+E++ TRYWCH+CSQ VNP+++ EIKCPFC+SGFVEEM +    D++   +  +NN   
Sbjct: 1   MEEAVVTRYWCHMCSQTVNPVMEAEIKCPFCQSGFVEEMEDDDDHDSSDPADVRANN--- 60

Query: 61  SDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAE 120
                    SLWAPIL+ +M +P  RRR + +E  ED+ N  + E+ + +G   +    +
Sbjct: 61  ---------SLWAPILMELMNDPVRRRRNQSVESVEDNQNEVQTENNEDDGE--NDLDWQ 120

Query: 121 LDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILIN 180
           L  ++RRRRR SA +LQLLQGIRAG++  ESE++  G  +              RVILIN
Sbjct: 121 LQEILRRRRRHSAAVLQLLQGIRAGLSV-ESESTGNGGNN------------PGRVILIN 180

Query: 181 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 240
             NQTI VQ     +    GSLGDYF+GPG ++LLQ LAENDPNRYGTPPA+KEAV+AL 
Sbjct: 181 TSNQTITVQNSADMDSVPAGSLGDYFIGPGFEMLLQRLAENDPNRYGTPPAKKEAVEALA 240

Query: 241 TVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPAD 300
           TV++E     LQCSVCLD+FE+  EAK MPC HKFHS C+LPWLELHSSCPVCR+QLPAD
Sbjct: 241 TVKIE---ETLQCSVCLDDFEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPAD 300

Query: 301 ESKRDT------DGGSARFMGELSNGNDSGSGS--------GSGEIDGRTASGIGRRFSF 360
           E+K D+      + GS+      S+G ++  G+           E +    +  G  FS 
Sbjct: 301 EAKTDSVTTTSDNNGSSSASATTSHGAENSDGNRRQEEEEEEEEEEEEENENNDGSGFSI 350

Query: 361 PWPFNGLFSSQGNSSGGSES 367
           PWPF+ LFSS  +S+  ++S
Sbjct: 361 PWPFSTLFSSSQDSNAPTDS 350

[Close]

BLAST of CmaCh20G000050 vs. TAIR 10
Match: AT4G26400.2 (RING/U-box superfamily protein )

HSP 1 Score: 314.3 bits (804), Expect = 1.3e-85
Identity = 186/387 (48.06%), Postives = 239/387 (61.76%), Query Frame = 0


Query: 5   IATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNAL--ESD 64
           ++ RYWCH+CSQMVNP++  EIKCPFC+SGFVEE    MS + NG ++++       E D
Sbjct: 1   MSARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEE----MSREINGGSSSSLREVQDPEID 60

Query: 65  FGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDG------------- 124
           FG++RALSLW PILLGMM NPR RRRFR  EF  D+D  N  +  DG             
Sbjct: 61  FGTDRALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRR 120

Query: 125 ----EGNRASSEPAELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERER 184
               +  R      E +S++RRRRRSSATILQLLQGIRAG+A+ E E+S           
Sbjct: 121 HRHRQQGREIDLDREFESILRRRRRSSATILQLLQGIRAGIAS-EYESS----------- 180

Query: 185 ERERERERERVILINPFNQTIVVQGGESQNHN----AIGSLGDYFVGPGLDLLLQHLAEN 244
                   +R +L    NQ+ VVQG  S N N    ++ ++GDYFVG  LD LL+HLA+N
Sbjct: 181 --------DRDLL----NQSAVVQGSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADN 240

Query: 245 DPNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCIL 304
           D  R+G+ PA+KE VD LPTV++      LQCS+CLD+F+   EAKEMPCKHKFH  CI+
Sbjct: 241 DSIRHGSLPARKEVVDNLPTVKI---SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIV 300

Query: 305 PWLELHSSCPVCRHQLPADESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIG 364
           PWLELHSSCPVCR++LP D+  +           E++  N+      + E D R ++   
Sbjct: 301 PWLELHSSCPVCRYELPPDDETKVNPVRPRTRTLEINVSNE------NVEDDARNSNVSE 350

Query: 365 RRFSFPWPFNGLFSSQGNSSGGSESES 369
           RRFSFPWPF+GLFSS  +SS  +   S
Sbjct: 361 RRFSFPWPFSGLFSSSSSSSASASGSS 350

[Close]

BLAST of CmaCh20G000050 vs. TAIR 10
Match: AT4G26400.1 (RING/U-box superfamily protein )

HSP 1 Score: 314.3 bits (804), Expect = 1.3e-85
Identity = 186/387 (48.06%), Postives = 239/387 (61.76%), Query Frame = 0


Query: 5   IATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNAL--ESD 64
           ++ RYWCH+CSQMVNP++  EIKCPFC+SGFVEE    MS + NG ++++       E D
Sbjct: 1   MSARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEE----MSREINGGSSSSLREVQDPEID 60

Query: 65  FGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDG------------- 124
           FG++RALSLW PILLGMM NPR RRRFR  EF  D+D  N  +  DG             
Sbjct: 61  FGTDRALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRR 120

Query: 125 ----EGNRASSEPAELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERER 184
               +  R      E +S++RRRRRSSATILQLLQGIRAG+A+ E E+S           
Sbjct: 121 HRHRQQGREIDLDREFESILRRRRRSSATILQLLQGIRAGIAS-EYESS----------- 180

Query: 185 ERERERERERVILINPFNQTIVVQGGESQNHN----AIGSLGDYFVGPGLDLLLQHLAEN 244
                   +R +L    NQ+ VVQG  S N N    ++ ++GDYFVG  LD LL+HLA+N
Sbjct: 181 --------DRDLL----NQSAVVQGSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADN 240

Query: 245 DPNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCIL 304
           D  R+G+ PA+KE VD LPTV++      LQCS+CLD+F+   EAKEMPCKHKFH  CI+
Sbjct: 241 DSIRHGSLPARKEVVDNLPTVKI---SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIV 300

Query: 305 PWLELHSSCPVCRHQLPADESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIG 364
           PWLELHSSCPVCR++LP D+  +           E++  N+      + E D R ++   
Sbjct: 301 PWLELHSSCPVCRYELPPDDETKVNPVRPRTRTLEINVSNE------NVEDDARNSNVSE 350

Query: 365 RRFSFPWPFNGLFSSQGNSSGGSESES 369
           RRFSFPWPF+GLFSS  +SS  +   S
Sbjct: 361 RRFSFPWPFSGLFSSSSSSSASASGSS 350

[Close]

BLAST of CmaCh20G000050 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 239.2 bits (609), Expect = 5.4e-63
Identity = 151/338 (44.67%), Postives = 201/338 (59.47%), Query Frame = 0


Query: 1   MDESIATRYWCHVCSQMVNPIVDVE-IKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNNAL 60
           M+++  T YWCH+CS+ V P++  E IKC FC+SGFVEEM      DNN ++        
Sbjct: 1   MEDASETSYWCHMCSRSVIPLIQDEIIKCNFCQSGFVEEM------DNNDDH-------- 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLE----FDEDDDNNNEQEHGDGEGNRAS 120
                 + A SLW PIL+ MM N   +      +     +++D++ ++ + GD   +   
Sbjct: 61  ------QAADSLWTPILMEMMNNNHDQHSTNQEDSESILEDEDEDEDDGDDGDQNNDGEI 120

Query: 121 SEPAELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERER 180
               +L+ + R R R S  I+ LLQGIRAG+   ESEN+             +     E 
Sbjct: 121 DITHQLEEIRRIRTRHSTAIVNLLQGIRAGLLI-ESENN------------EDNPDNSEL 180

Query: 181 VILINPFNQTI-VVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAEND-PNRYGTPPAQK 240
           V+LIN FNQ I V Q          GSLGDYF+GPG + LLQ LAEND  NRYGTPPA K
Sbjct: 181 VVLINSFNQRIRVHQDSVDTTSVPSGSLGDYFIGPGFETLLQRLAENDLNNRYGTPPATK 240

Query: 241 EAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVC 300
           EAV+AL  V++E  D+ LQCSVCLD+FE+  EAKEMPCKHKFHS C+LPWLELHSSCPVC
Sbjct: 241 EAVEALAMVKIE--DSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVC 300

Query: 301 RHQLP-ADESKRDTDGGSAR---FMGELSNGNDSGSGS 328
           R+ LP  D+ +  TD  ++R      ++SN + + +GS
Sbjct: 301 RYLLPTGDDDEPKTDAETSRNDDNNEDISNASMASNGS 303

The following BLAST results are available for this feature:

BLAST of CmaCh20G000050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6AVN2	5.1e-50	36.62	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q8LPN7	1.4e-36	39.84	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	4.6e-35	32.53	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
O22197	5.1e-26	38.42	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q94AK4	1.1e-23	32.80	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]

BLAST of CmaCh20G000050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JDY5	1.7e-213	100.00	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 P...	[more]
A0A6J1JBZ6	2.2e-205	97.07	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 P...	[more]
A0A6J1FWF9	7.5e-198	94.41	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781...	[more]
A0A1S3BNR7	2.7e-171	83.42	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 ...	[more]
A0A5D3DDU3	2.7e-171	83.42	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of CmaCh20G000050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022985674.1	3.5e-213	100.00	E3 ubiquitin-protein ligase SIRP1-like isoform X1 [Cucurbita maxima]	[more]
XP_022985675.1	4.5e-205	97.07	E3 ubiquitin-protein ligase SIRP1-like isoform X2 [Cucurbita maxima]	[more]
KAG7010206.1	4.4e-200	95.20	E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022944289.1	1.6e-197	94.41	E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata]	[more]
XP_023512553.1	3.5e-197	93.37	E3 ubiquitin-protein ligase SIRP1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh20G000050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56340.1	7.4e-105	55.28	RING/U-box superfamily protein	[more]
AT1G55530.1	2.0e-86	47.89	RING/U-box superfamily protein	[more]
AT4G26400.2	1.3e-85	48.06	RING/U-box superfamily protein	[more]
AT4G26400.1	1.3e-85	48.06	RING/U-box superfamily protein	[more]
AT3G13430.1	5.4e-63	44.67	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 253..293 e-value: 7.0E-8 score: 42.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 252..294 e-value: 8.9E-13 score: 48.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 253..294 score: 12.40076
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 7..39 e-value: 1.2E-11 score: 44.6
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 217..300 e-value: 7.9E-21 score: 75.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 350..375
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 147..171
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 154..171
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 299..375
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 105..126
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 93..126
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 316..332
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 3..353
None	No IPR available	PANTHER	PTHR15710:SF22	RING/U-BOX SUPERFAMILY PROTEIN	coord: 3..353
None	No IPR available	CDD	cd16669	RING-H2_RNF181	coord: 252..297 e-value: 1.10753E-23 score: 90.5077
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 248..300

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh20G000050.1	CmaCh20G000050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016020	membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity