CmaCh19G010300 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh19G010300
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionKetol-acid reductoisomerase
LocationCma_Chr19: 8794891 .. 8798930 (-)
RNA-Seq ExpressionCmaCh19G010300
SyntenyCmaCh19G010300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGTAGGTGGATATCAATTCTGTGTACGCGAGGCTTAGAATGCACCAGCCTTTGGTACCCTATATTAAACCGTGCAACCTCTGCTTTCTTTATCATCCCCCACATTTGCTGACTCTACTCATCAATGGCCGCCGCCACATATTTCTCTCCCTCTATCTCAGCCTCATTATCAAAAACCCTAAAACAAGCTGCTTCTCTACATCCCCTTACTCCTTCCAGCTTAGGATTCTTGTCCTCCTCCTCCGCTTCCTCGGGATTCTTCGCTCTTAAGGCTGTTGGTATGGGTGGTGCTAATGCTGTCGGCTCAGCTCTTGGTGCTCGCATGGTGTCTGCACCTGCGATCAAGCCCTTAATCTCGCTTGATTTTGACACATCTGTGTTCAAGAAGGAGAAAGTTAATCTCGCTGGCCATGATGAGGTTTTTACTCTGTTACTTGTACCCTTGTGTATTGATAGGTTCTCTGGTTTTGGATTTGGATTTCGGGGAAGTGAGCTGTGAGTGTGGATTGTGGAAGGGAAATGGATTCGTGTGCATCCATTTCTTGAGATCTGCATGTCTTTTTTTTTTCTTTTTTTTTTTATTCTCTGCATGTCTTCGCTTTGTGGAAGCGAAATGTTTCTGTATTGGAAATGGGAATTTAATTGTTTCATTTCCGTTTGTGTGCCTTCGGATTGCAGTATATTGTCAGAGGAGGCAGGGACCTTTTTCCCCTGCTGCCGGAAGCGTTCAAGGGGATCAAGAAAATTGGTGTCATTGGTTGGGGTTCACAGGTAATGTTATATCACGATCTGCAATCTTATATTTGTCATGTTTTGTTGCAGAATCAGCCTGCAATCTTATATTTCACATGGGATTTGATTTTTATGTTCATAATAATAGAAGAAAATGATGTGGCATTTTACAAAATCAAGGATAAATGGGTAAAAATATTACAACATTCAACCTCCATTCAAGGATTTTCTTTTTTCACTTATCAGCATCTGATTGATATGAACAAAAAAAGAAAAAGGATGAAATGTAACAAAGTGACCAACAAGGTTCTAGGAATGCGTTCTCTAACAATGTTTCTTTGGGGTGGAAATTAGAGCACTAAATTTTTGAGAAACTGAGCTGACGTTTGCATCTGAAGTTAGAATGTATACACATGGCTCTGGTTCATGAAGAACTCTCTCAGACAAGCGCTCTCTAGAAGCCTATAGGAGACATCTCTTTTCAGAAAGACGCCAAATTTTCTAACCCTTTTATTGATTCTCTATCTAACCGTAGTTTATCCGTGTGCATGCATTTGTTCGATCAGGCACCTGCACAAGCTCAGAATTTAAGGGACTCGCTTGCTGAAGCCAAGTCTGATATTGTGGTTAAGGTGATTTTCTTTATCTCGTGGTTAAGGTGTGCATTATAGGCTGTGATGTTGTATTATTGATCAATCCCTTATATAAACTTCGATATTTCCATTTGATTTTTGTTGATACAGATTGGCCTGAGGAAGGGTTCCCGCTCTTTTGCGGAAGCTCGCACAGTTGGTTTTTCTGAAGAGAATGGGACTTTGGGAGATATATATGAAACCATTTCCGAAAGTGATCTTGTCATGCTTCTGATTTCTGATTCTGCGCAGGTCAGTTGGTTGAAAATATCCCAACTTTGTTGCTTCTGGTACTATTAAGCTAAAGTCTTGACTATATGAGACCTGAGTTTCGATTAAGTGCTTGTGATAGTTGATACATATGATTGTTTGATTATCTGTATTCCGTGCTTTGGAACGTTTTTGTAGAATTTGCAAATTTCTTACTGTCGTTGTACTTTGCAGGCTGATAACTATGAAAAAATATTTTCTCACATGAAACCAAACAGCATTCTTGGGCTTTCCCATGGGTTTCTTCTTGGCCATTTGCAGTCATTGGGACTTGATTTCCCTAAGAATTTTAGCGTGATTGCTGTTTGTCCAAAGGGGATGGGTCCATCTGTGAGGAGACTGTATGTTCAAGGAAAAGAGATAAATGGTGCAGGAATTAATTCTAGTTTTGCAGTTCACCAGGTAATATTGCATTCTCCAATTTGAGCGGATATCGATTAGTGTACTTGGTTGCTAATTATGTACCGTTTACAGGATGTCGATGGTAGAGCCACCGATGTTGCATTGGGATGGTCTGTTGCCCTTGGCTCTCCTTTCACATTCGCTACAACTCTAGAGCAAGAATACAGGAGTGACATCTTCGGCGAGCGAGGTAAACTTGCCACTCTTCGTATTGTGTACATTCAAGTATGCACAAATTTTGAATACCCAACTTCTTCTGCTGTGGTTGATAGGTATTCTACTTGGTGCTGTTCATGGAATTGTGGAATCCTTGTTCAGAAGGTACACTGAAAATGGAATGGCTGAAGACCTGGCTTATAAGAACACTGTTGAAAGCATAACAGGGATCATATCGAAAACCATTTCCACTAAGGTTGTATCTGCTTACTAGATTTAACTCTCAGTAACCTTCCATGCATGCACTTGCTTCATATTAATGGCCGTCTGTTTTTCTTACTACGTAGGGAATGTTGGCAGTATACAATTCCTTCTCTCCTGATGAGAAAAAGGAATTCGAGAGAGCATATAGTGCATCGTTTTACCCTTGTATGGATATTCTCTATGAATGTTATGAAGATGTTGCCAGTGGAAGTGAGATCCGCAGCGTTGTGCTTGCTGGCCGACGATTTTATGTATGTTGGTACCTTTTTTTCTTTTCCTTCACAGCAAAAATATTTGATGGAACCAGTATAGTCTGTAGTGTAAACGAGCTATTCGCCCGTGATCTTTATAATTCTAGTCGAATAATCGTTTTGGTGCCTGTTATGCTTCATAACTTGACCTGCCTTATTATTACAGGAAAAGGAGGGTCTCCCAGCTTTCCCAATGGGGAACATTGACCAGACCCGGATGTGGAAAGTTGGTGAACGTGTTCGACAATCACGTCCTGCAGGTGACCTCGGCCCATTATATCCTTTCACTGCTGGTGTATATGTGGCGTTGATGATGGCACAGGTATGGTAGTCTGATGACAATTTTGGACTCTCGAACTTTAAAATCTATTCGAATGGATTGAACTCAGTAGTTATGAACACGACTTGCTGACTCGTATACCTTAAAAGTTTATTATATACCATTGGTTATGGAAACGAAGATCTGTCTGCCTGCTTCATAATAAAATTCTATTTTCAGATCGAGATACTGAGGAAGAAGGGCCACTCGTACTCAGAGATCATCAACGAAAGTGTAATTGAATCAGTGGACTCTTTGAACCCATTCATGCATGCTCGTGGTGTTTCATTCATGGTCGACAACTGTTCCACCACAGCGCGATTGGGATCTCGAAAGTGGGCTCCTCGCTTCGACTACATTCTCACCCAACAAGCCCTGGTTGCCATTGACAATGGTTCAGCCGTCAACCAAGACCTTATCAGCAACTTCCTGTCCGACCCAGTTCATGGAGCAATTGAAGTATGCGCCCAGTTGAGACCTACTGTTGACATTTCTGTTCCAGCTGATGCTGATTTTGTTCGACCAGAACTACGTCAATCTAGCAACTAAAATGTGCCAACGATGCCCGTGAACTAGGTTAATCGCGTTCCACGAAGGACTGCTGTATGTATCTACGAAATATGTACTGTTATCCTGAGTTTCTTCGAGATCTAGTTTTAAGTCTATGATAAGAGTTAGTTGTGCGAGTGGTAGTAATAAGTTGAATGTTCCAGACTTTTTCGGGTCGTTGTCTATGTCGTTGTAATGTAGACTTTCGGGATCGTGTTGGAATTATGAGCTACTACTATTATGGTATCTTATATTAGTTTCATCCTAAACTCCCACTCTAGTGACAGCTTCCATGCACTTCACGCTCGTGGAATAACTATTTGAGAACCTTGTGAAGTTGCCCATAATAAGCTGTAAATCGGTCTGATTTGGTCTTCAACTGAATCGATTACTTAATTTGGTTTGATATCGACCATGGATTTGGGCTCGGAAAGAATCATCTTCTCATGGTCTATAGGAACTAGG

mRNA sequence

TGGTAGGTGGATATCAATTCTGTGTACGCGAGGCTTAGAATGCACCAGCCTTTGGTACCCTATATTAAACCGTGCAACCTCTGCTTTCTTTATCATCCCCCACATTTGCTGACTCTACTCATCAATGGCCGCCGCCACATATTTCTCTCCCTCTATCTCAGCCTCATTATCAAAAACCCTAAAACAAGCTGCTTCTCTACATCCCCTTACTCCTTCCAGCTTAGGATTCTTGTCCTCCTCCTCCGCTTCCTCGGGATTCTTCGCTCTTAAGGCTGTTGGTATGGGTGGTGCTAATGCTGTCGGCTCAGCTCTTGGTGCTCGCATGGTGTCTGCACCTGCGATCAAGCCCTTAATCTCGCTTGATTTTGACACATCTGTGTTCAAGAAGGAGAAAGTTAATCTCGCTGGCCATGATGAGTATATTGTCAGAGGAGGCAGGGACCTTTTTCCCCTGCTGCCGGAAGCGTTCAAGGGGATCAAGAAAATTGGTGTCATTGGTTGGGGTTCACAGGCACCTGCACAAGCTCAGAATTTAAGGGACTCGCTTGCTGAAGCCAAGTCTGATATTGTGGTTAAGATTGGCCTGAGGAAGGGTTCCCGCTCTTTTGCGGAAGCTCGCACAGTTGGTTTTTCTGAAGAGAATGGGACTTTGGGAGATATATATGAAACCATTTCCGAAAGTGATCTTGTCATGCTTCTGATTTCTGATTCTGCGCAGGCTGATAACTATGAAAAAATATTTTCTCACATGAAACCAAACAGCATTCTTGGGCTTTCCCATGGGTTTCTTCTTGGCCATTTGCAGTCATTGGGACTTGATTTCCCTAAGAATTTTAGCGTGATTGCTGTTTGTCCAAAGGGGATGGGTCCATCTGTGAGGAGACTGTATGTTCAAGGAAAAGAGATAAATGGTGCAGGAATTAATTCTAGTTTTGCAGTTCACCAGGATGTCGATGGTAGAGCCACCGATGTTGCATTGGGATGGTCTGTTGCCCTTGGCTCTCCTTTCACATTCGCTACAACTCTAGAGCAAGAATACAGGAGTGACATCTTCGGCGAGCGAGGTATTCTACTTGGTGCTGTTCATGGAATTGTGGAATCCTTGTTCAGAAGGTACACTGAAAATGGAATGGCTGAAGACCTGGCTTATAAGAACACTGTTGAAAGCATAACAGGGATCATATCGAAAACCATTTCCACTAAGGGAATGTTGGCAGTATACAATTCCTTCTCTCCTGATGAGAAAAAGGAATTCGAGAGAGCATATAGTGCATCGTTTTACCCTTGTATGGATATTCTCTATGAATGTTATGAAGATGTTGCCAGTGGAAGTGAGATCCGCAGCGTTGTGCTTGCTGGCCGACGATTTTATGAAAAGGAGGGTCTCCCAGCTTTCCCAATGGGGAACATTGACCAGACCCGGATGTGGAAAGTTGGTGAACGTGTTCGACAATCACGTCCTGCAGGTGACCTCGGCCCATTATATCCTTTCACTGCTGGTGTATATGTGGCGTTGATGATGGCACAGATCGAGATACTGAGGAAGAAGGGCCACTCGTACTCAGAGATCATCAACGAAAGTGTAATTGAATCAGTGGACTCTTTGAACCCATTCATGCATGCTCGTGGTGTTTCATTCATGGTCGACAACTGTTCCACCACAGCGCGATTGGGATCTCGAAAGTGGGCTCCTCGCTTCGACTACATTCTCACCCAACAAGCCCTGGTTGCCATTGACAATGGTTCAGCCGTCAACCAAGACCTTATCAGCAACTTCCTGTCCGACCCAGTTCATGGAGCAATTGAAGTATGCGCCCAGTTGAGACCTACTGTTGACATTTCTGTTCCAGCTGATGCTGATTTTGTTCGACCAGAACTACGTCAATCTAGCAACTAAAATGTGCCAACGATGCCCGTGAACTAGGTTAATCGCGTTCCACGAAGGACTGCTGTATGTATCTACGAAATATGTACTGTTATCCTGAGTTTCTTCGAGATCTAGTTTTAAGTCTATGATAAGAGTTAGTTGTGCGAGTGGTAGTAATAAGTTGAATGTTCCAGACTTTTTCGGGTCGTTGTCTATGTCGTTGTAATGTAGACTTTCGGGATCGTGTTGGAATTATGAGCTACTACTATTATGGTATCTTATATTAGTTTCATCCTAAACTCCCACTCTAGTGACAGCTTCCATGCACTTCACGCTCGTGGAATAACTATTTGAGAACCTTGTGAAGTTGCCCATAATAAGCTGTAAATCGGTCTGATTTGGTCTTCAACTGAATCGATTACTTAATTTGGTTTGATATCGACCATGGATTTGGGCTCGGAAAGAATCATCTTCTCATGGTCTATAGGAACTAGG

Coding sequence (CDS)

ATGGCCGCCGCCACATATTTCTCTCCCTCTATCTCAGCCTCATTATCAAAAACCCTAAAACAAGCTGCTTCTCTACATCCCCTTACTCCTTCCAGCTTAGGATTCTTGTCCTCCTCCTCCGCTTCCTCGGGATTCTTCGCTCTTAAGGCTGTTGGTATGGGTGGTGCTAATGCTGTCGGCTCAGCTCTTGGTGCTCGCATGGTGTCTGCACCTGCGATCAAGCCCTTAATCTCGCTTGATTTTGACACATCTGTGTTCAAGAAGGAGAAAGTTAATCTCGCTGGCCATGATGAGTATATTGTCAGAGGAGGCAGGGACCTTTTTCCCCTGCTGCCGGAAGCGTTCAAGGGGATCAAGAAAATTGGTGTCATTGGTTGGGGTTCACAGGCACCTGCACAAGCTCAGAATTTAAGGGACTCGCTTGCTGAAGCCAAGTCTGATATTGTGGTTAAGATTGGCCTGAGGAAGGGTTCCCGCTCTTTTGCGGAAGCTCGCACAGTTGGTTTTTCTGAAGAGAATGGGACTTTGGGAGATATATATGAAACCATTTCCGAAAGTGATCTTGTCATGCTTCTGATTTCTGATTCTGCGCAGGCTGATAACTATGAAAAAATATTTTCTCACATGAAACCAAACAGCATTCTTGGGCTTTCCCATGGGTTTCTTCTTGGCCATTTGCAGTCATTGGGACTTGATTTCCCTAAGAATTTTAGCGTGATTGCTGTTTGTCCAAAGGGGATGGGTCCATCTGTGAGGAGACTGTATGTTCAAGGAAAAGAGATAAATGGTGCAGGAATTAATTCTAGTTTTGCAGTTCACCAGGATGTCGATGGTAGAGCCACCGATGTTGCATTGGGATGGTCTGTTGCCCTTGGCTCTCCTTTCACATTCGCTACAACTCTAGAGCAAGAATACAGGAGTGACATCTTCGGCGAGCGAGGTATTCTACTTGGTGCTGTTCATGGAATTGTGGAATCCTTGTTCAGAAGGTACACTGAAAATGGAATGGCTGAAGACCTGGCTTATAAGAACACTGTTGAAAGCATAACAGGGATCATATCGAAAACCATTTCCACTAAGGGAATGTTGGCAGTATACAATTCCTTCTCTCCTGATGAGAAAAAGGAATTCGAGAGAGCATATAGTGCATCGTTTTACCCTTGTATGGATATTCTCTATGAATGTTATGAAGATGTTGCCAGTGGAAGTGAGATCCGCAGCGTTGTGCTTGCTGGCCGACGATTTTATGAAAAGGAGGGTCTCCCAGCTTTCCCAATGGGGAACATTGACCAGACCCGGATGTGGAAAGTTGGTGAACGTGTTCGACAATCACGTCCTGCAGGTGACCTCGGCCCATTATATCCTTTCACTGCTGGTGTATATGTGGCGTTGATGATGGCACAGATCGAGATACTGAGGAAGAAGGGCCACTCGTACTCAGAGATCATCAACGAAAGTGTAATTGAATCAGTGGACTCTTTGAACCCATTCATGCATGCTCGTGGTGTTTCATTCATGGTCGACAACTGTTCCACCACAGCGCGATTGGGATCTCGAAAGTGGGCTCCTCGCTTCGACTACATTCTCACCCAACAAGCCCTGGTTGCCATTGACAATGGTTCAGCCGTCAACCAAGACCTTATCAGCAACTTCCTGTCCGACCCAGTTCATGGAGCAATTGAAGTATGCGCCCAGTTGAGACCTACTGTTGACATTTCTGTTCCAGCTGATGCTGATTTTGTTCGACCAGAACTACGTCAATCTAGCAACTAA

Protein sequence

MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVGSALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTKGMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNGSAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN
Homology
BLAST of CmaCh19G010300 vs. ExPASy Swiss-Prot
Match: Q05758 (Ketol-acid reductoisomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g58610 PE=1 SV=2)

HSP 1 Score: 977.2 bits (2525), Expect = 8.0e-284
Identity = 503/595 (84.54%), Postives = 540/595 (90.76%), Query Frame = 0

Query: 1   MAAAT-YFSPSIS----ASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGG 60
           MAAAT   +PS+S    +S SKTL  ++    L   ++GFLSSSS S         G G 
Sbjct: 1   MAAATSSIAPSLSCPSPSSSSKTL-WSSKARTLALPNIGFLSSSSKSLRSLTATVAGNG- 60

Query: 61  ANAVGSALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAF 120
             A GS+L ARMVS+ A+K  +SLDF+TSVFKKEKV+LAG++EYIVRGGRDLF  LP+AF
Sbjct: 61  --ATGSSLAARMVSSSAVKAPVSLDFETSVFKKEKVSLAGYEEYIVRGGRDLFKHLPDAF 120

Query: 121 KGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGT 180
           KGIK+IGVIGWGSQ PAQAQNLRDSL EAKSDIVVKIGLRKGSRSF EAR  GF+EE+GT
Sbjct: 121 KGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKIGLRKGSRSFEEARAAGFTEESGT 180

Query: 181 LGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPK 240
           LGDI+ETI+ SDLV+LLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GLDFPK
Sbjct: 181 LGDIWETIAGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLDFPK 240

Query: 241 NFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPF 300
           N SV+AVCPKGMGPSVRRLYVQGKEINGAGIN+SFAVHQDVDGRA DVALGWSVALGSPF
Sbjct: 241 NISVVAVCPKGMGPSVRRLYVQGKEINGAGINASFAVHQDVDGRAADVALGWSVALGSPF 300

Query: 301 TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISK 360
           TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+EDLAYKNTVE ITG IS+
Sbjct: 301 TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVECITGTISR 360

Query: 361 TISTKGMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRF 420
           TIST+GMLAVYNS S + KK+FE AYSASFYPCM+ILYECYEDV SGSEIRSVVLAGRRF
Sbjct: 361 TISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGSEIRSVVLAGRRF 420

Query: 421 YEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKK 480
           YEKEGLPAFPMGNIDQTRMWKVGERVR+SRPAGDLGPLYPFTAGVYVALMMAQIEILRKK
Sbjct: 421 YEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKK 480

Query: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV 540
           GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV
Sbjct: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV 540

Query: 541 AIDNGSAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           A+D+G+A+N+DLISNF SDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN
Sbjct: 541 AVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591

BLAST of CmaCh19G010300 vs. ExPASy Swiss-Prot
Match: Q01292 (Ketol-acid reductoisomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=AHRI PE=1 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 1.6e-279
Identity = 495/594 (83.33%), Postives = 533/594 (89.73%), Query Frame = 0

Query: 3   AATYF----SPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGM--GGA 62
           AAT F    S S SA+ SK LKQ+     L   +LGFL SSS      +LKA  +   GA
Sbjct: 5   AATTFSLSSSSSTSAAASKALKQSPKPSAL---NLGFLGSSSTIKACRSLKAARVLPSGA 64

Query: 63  NAVGSALGARMVSAPAIK--PLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEA 122
           N  GSAL A+MVSAP+I      + DFD+SVFKKEKV L+GHDEYIVRGGR+LFPLLP+A
Sbjct: 65  NGGGSALSAQMVSAPSINTPSATTFDFDSSVFKKEKVTLSGHDEYIVRGGRNLFPLLPDA 124

Query: 123 FKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENG 182
           FKGIK+IGVIGWGSQAPAQAQNL+DSL EAKSD+VVKIGLRKGS SFAEAR  GFSEENG
Sbjct: 125 FKGIKQIGVIGWGSQAPAQAQNLKDSLTEAKSDVVVKIGLRKGSNSFAEARAAGFSEENG 184

Query: 183 TLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFP 242
           TLGD++ETIS SDLV+LLISDSAQADNYEK+FSHMKPNSILGLSHGFLLGHLQSLG DFP
Sbjct: 185 TLGDMWETISGSDLVLLLISDSAQADNYEKVFSHMKPNSILGLSHGFLLGHLQSLGQDFP 244

Query: 243 KNFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSP 302
           KN SVIAVCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWS+ALGSP
Sbjct: 245 KNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSIALGSP 304

Query: 303 FTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIIS 362
           FTFATTLEQEY+SDIFGERGILLGAVHGIVE LFRRYTE+GM+EDLAYKNTVE ITG+IS
Sbjct: 305 FTFATTLEQEYKSDIFGERGILLGAVHGIVECLFRRYTESGMSEDLAYKNTVECITGVIS 364

Query: 363 KTISTKGMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRR 422
           KTISTKGMLA+YNS S + KK+F+ AYSAS+YP MDILYECYEDVASGSEIRSVVLAGRR
Sbjct: 365 KTISTKGMLALYNSLSEEGKKDFQAAYSASYYPSMDILYECYEDVASGSEIRSVVLAGRR 424

Query: 423 FYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRK 482
           FYEKEGLPAFPMG IDQTRMWKVGE+VR  RPAGDLGPLYPFTAGVYVALMMAQIEILRK
Sbjct: 425 FYEKEGLPAFPMGKIDQTRMWKVGEKVRSVRPAGDLGPLYPFTAGVYVALMMAQIEILRK 484

Query: 483 KGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQAL 542
           KGHSYSEIINESVIE+VDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIL+QQAL
Sbjct: 485 KGHSYSEIINESVIEAVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILSQQAL 544

Query: 543 VAIDNGSAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQS 589
           VA+DNG+ +NQDLISNFLSDPVH AI VCAQLRP+VDISV ADADFVRPELRQ+
Sbjct: 545 VAVDNGAPINQDLISNFLSDPVHEAIGVCAQLRPSVDISVTADADFVRPELRQA 595

BLAST of CmaCh19G010300 vs. ExPASy Swiss-Prot
Match: O82043 (Ketol-acid reductoisomerase, chloroplastic OS=Pisum sativum OX=3888 GN=PGAAIR PE=2 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 2.7e-279
Identity = 489/590 (82.88%), Postives = 529/590 (89.66%), Query Frame = 0

Query: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60
           MAA T    +  ++ SKTL +  +     P++L F   S  S        +    +  VG
Sbjct: 1   MAAVTSSCSTAISASSKTLAKPVAA-SFAPTNLSFSKLSPQS--------IRARRSITVG 60

Query: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120
           SALGA  VSAP     +SLDF+TSVFKKE+VNLAGH+EYIVRGGRDLF LLP+AFKGIK+
Sbjct: 61  SALGATKVSAPPATHPVSLDFETSVFKKERVNLAGHEEYIVRGGRDLFHLLPDAFKGIKQ 120

Query: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY 180
           IGVIGWGSQ PAQAQNLRDSL EAKSDIVVK+GLRKGS SF EAR  GFSEE GTLGDI+
Sbjct: 121 IGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKVGLRKGSSSFNEAREAGFSEEKGTLGDIW 180

Query: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ETIS SDLV+LLISDSAQADNYEKIFSH+KPNSILGLSHGFLLGHLQS+GLDFPKNFSVI
Sbjct: 181 ETISGSDLVLLLISDSAQADNYEKIFSHLKPNSILGLSHGFLLGHLQSIGLDFPKNFSVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300
           AVCPKGMGPSVRRLYVQGKEINGAGINSSF VHQDVDGRAT+VALGWSVALGSPFTFATT
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFGVHQDVDGRATNVALGWSVALGSPFTFATT 300

Query: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360
           LEQEY+SDIFGERGILLGAVHGIVESLFRRYTENGM+EDLAYKNTVESITG+ISKTIST+
Sbjct: 301 LEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITGVISKTISTQ 360

Query: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYN+ S D KKEFE+AYSASFYPCM+ILYECYEDVASGSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNALSEDGKKEFEKAYSASFYPCMEILYECYEDVASGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMG IDQTRMWKVGERVR +RPAGDLGPLYPFTAGV+VA+MMAQIE+LRKKGHSYS
Sbjct: 421 LPAFPMGKIDQTRMWKVGERVRSTRPAGDLGPLYPFTAGVFVAMMMAQIEVLRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVA+D+G
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDSG 540

Query: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           + +NQDLISNF+SDPVHGAI+VCA+LRPT+DISVPA ADFVRPELRQ SN
Sbjct: 541 APINQDLISNFVSDPVHGAIQVCAELRPTLDISVPAAADFVRPELRQCSN 581

BLAST of CmaCh19G010300 vs. ExPASy Swiss-Prot
Match: Q65XK0 (Ketol-acid reductoisomerase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0573700 PE=1 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 2.7e-271
Identity = 477/590 (80.85%), Postives = 520/590 (88.14%), Query Frame = 0

Query: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60
           MAA+T    +++ S  KTL  AA+  P  P++   +S        F +         A  
Sbjct: 1   MAAST----TLALSHPKTLAAAAAAAPKAPTAPAAVS--------FPVSHAACAPLAARR 60

Query: 61  SALGARMVSAPAI-KPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIK 120
            A+ A + + PA+   + SLDFDTSVF KEKV+LAGH+EYIVRGGR+LFPLLPEAFKGIK
Sbjct: 61  RAVTAMVAAPPAVGAAMPSLDFDTSVFNKEKVSLAGHEEYIVRGGRNLFPLLPEAFKGIK 120

Query: 121 KIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDI 180
           +IGVIGWGSQ PAQAQNLRDSLAEAKSDIVVKIGLRKGS+SF EAR  GF+EE+GTLGDI
Sbjct: 121 QIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSKSFDEARAAGFTEESGTLGDI 180

Query: 181 YETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSV 240
           +ET+S SDLV+LLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GLDFPKN SV
Sbjct: 181 WETVSGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSAGLDFPKNISV 240

Query: 241 IAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFAT 300
           IAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFAT
Sbjct: 241 IAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFAT 300

Query: 301 TLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTIST 360
           TLEQEY+SDIFGERGILLGAVHGIVE+LFRRYTE GM E++AYKNTVE ITGIISKTIS 
Sbjct: 301 TLEQEYKSDIFGERGILLGAVHGIVEALFRRYTEQGMDEEMAYKNTVEGITGIISKTISK 360

Query: 361 KGMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKE 420
           KGML VYNS + + KKEF +AYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKE
Sbjct: 361 KGMLEVYNSLTEEGKKEFNKAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKE 420

Query: 421 GLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSY 480
           GLPAFPMGNIDQTRMWKVGE+VR +RP  DLGPL+PFTAGVYVALMMAQIE+LRKKGHSY
Sbjct: 421 GLPAFPMGNIDQTRMWKVGEKVRSTRPENDLGPLHPFTAGVYVALMMAQIEVLRKKGHSY 480

Query: 481 SEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDN 540
           SEIINESVIESVDSLNPFMHARGV+FMVDNCSTTARLGSRKWAPRFDYILTQQA V +D 
Sbjct: 481 SEIINESVIESVDSLNPFMHARGVAFMVDNCSTTARLGSRKWAPRFDYILTQQAFVTVDK 540

Query: 541 GSAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSS 590
            + +NQDLISNF+SDPVHGAIEVCA+LRPTVDISVPA+ADFVRPELRQSS
Sbjct: 541 DAPINQDLISNFMSDPVHGAIEVCAELRPTVDISVPANADFVRPELRQSS 578

BLAST of CmaCh19G010300 vs. ExPASy Swiss-Prot
Match: P38674 (Ketol-acid reductoisomerase, mitochondrial OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=ilv-2 PE=3 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 7.5e-40
Identity = 121/400 (30.25%), Postives = 200/400 (50.00%), Query Frame = 0

Query: 53  MGGANAVGSALGARMVSAPA---IKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFP 112
           +  A+AV +++  + V+APA   ++ + ++DF            AGH E +         
Sbjct: 28  VAAASAVRASVAVKAVAAPARQQVRGVKTMDF------------AGHKEEVHERADWPAE 87

Query: 113 LLPEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGF 172
            L + FK    + +IG+GSQ   Q  NLRD      + + V +G+RK  +S+ +A   G+
Sbjct: 88  KLLDYFKN-DTLALIGYGSQGHGQGLNLRD------NGLNVIVGVRKNGKSWEDAIQDGW 147

Query: 173 SEENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSL 232
                 L D+ E IS   +VM L+SD+AQ++ +  I   +     L  SHGF        
Sbjct: 148 -VPGKNLFDVDEAISRGTIVMNLLSDAAQSETWPHIKPQITKGKTLYFSHGFSPVFKDLT 207

Query: 233 GLDFPKNFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSV 292
            ++ P +  VI V PKG G +VR L+ +G+     GINSSFAV+QDV G+A + A+   V
Sbjct: 208 KVEVPTDVDVILVAPKGSGRTVRSLFREGR-----GINSSFAVYQDVTGKAKEKAVALGV 267

Query: 293 ALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESI 352
           A+GS + + TT E+E  SD++GERG L+G +HG+  + +    E G +   A+  TVE  
Sbjct: 268 AVGSGYLYETTFEKEVYSDLYGERGCLMGGIHGMFLAQYEVLRERGHSPSEAFNETVEEA 327

Query: 353 TGIISKTISTKGMLAVYNSFSPDEKK---EFERAYSASFYPCMDILYECYEDVASGSEIR 412
           T  +   I   GM  ++++ S   ++   ++   +  +  P  + L   Y+ V +G E +
Sbjct: 328 TQSLYPLIGAHGMDWMFDACSTTARRGAIDWTPKFKDALKPVFNNL---YDSVKNGDETK 387

Query: 413 SVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRP 447
             +    +   +E   A  +  I    +W+ G+ VR  RP
Sbjct: 388 RSLEYNSQPDYRERYEA-ELDEIRNLEIWRAGKAVRSLRP 398

BLAST of CmaCh19G010300 vs. ExPASy TrEMBL
Match: A0A6J1HV87 (Ketol-acid reductoisomerase OS=Cucurbita maxima OX=3661 GN=LOC111468154 PE=3 SV=1)

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 590/590 (100.00%), Postives = 590/590 (100.00%), Query Frame = 0

Query: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60
           MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60

Query: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120
           SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120

Query: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY 180
           IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY
Sbjct: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY 180

Query: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI
Sbjct: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300
           AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300

Query: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360
           LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK
Sbjct: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360

Query: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS
Sbjct: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540

Query: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN
Sbjct: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of CmaCh19G010300 vs. ExPASy TrEMBL
Match: A0A6J1GKD7 (Ketol-acid reductoisomerase OS=Cucurbita moschata OX=3662 GN=LOC111455167 PE=3 SV=1)

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 586/590 (99.32%), Postives = 587/590 (99.49%), Query Frame = 0

Query: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60
           MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSG FALKAVGMGG NAVG
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGSFALKAVGMGGGNAVG 60

Query: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120
           SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIK+
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ 120

Query: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY 180
           IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEAR VGFSEENGTLGDIY
Sbjct: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIY 180

Query: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI
Sbjct: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300
           AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300

Query: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360
           LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK
Sbjct: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360

Query: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS
Sbjct: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540

Query: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN
Sbjct: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of CmaCh19G010300 vs. ExPASy TrEMBL
Match: A0A5D3C563 (Ketol-acid reductoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003030 PE=3 SV=1)

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 566/590 (95.93%), Postives = 579/590 (98.14%), Query Frame = 0

Query: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60
           MAAAT+F+PSISAS +KTLKQAA+L PL+PSSLGFLSSS  SSG +ALKAVG+ GAN VG
Sbjct: 1   MAAATHFAPSISASSTKTLKQAAALQPLSPSSLGFLSSS--SSGLYALKAVGICGANGVG 60

Query: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120
           SALGARMVS PAIKPL SLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK
Sbjct: 61  SALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120

Query: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY 180
           IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEAR VGFSEENGTLGDIY
Sbjct: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIY 180

Query: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKN SVI
Sbjct: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNISVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300
           AVCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWS+ALGSPFTFATT
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSIALGSPFTFATT 300

Query: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360
           LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAE+LAYKNTVESITGIIS+TISTK
Sbjct: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISRTISTK 360

Query: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYNSFSPDEKKEFERAYSAS+YPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS
Sbjct: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540

Query: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           SA+NQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN
Sbjct: 541 SAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 588

BLAST of CmaCh19G010300 vs. ExPASy TrEMBL
Match: A0A1S3C039 (Ketol-acid reductoisomerase OS=Cucumis melo OX=3656 GN=LOC103495472 PE=3 SV=1)

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 566/590 (95.93%), Postives = 579/590 (98.14%), Query Frame = 0

Query: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60
           MAAAT+F+PSISAS +KTLKQAA+L PL+PSSLGFLSSS  SSG +ALKAVG+ GAN VG
Sbjct: 1   MAAATHFAPSISASSTKTLKQAAALQPLSPSSLGFLSSS--SSGLYALKAVGICGANGVG 60

Query: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120
           SALGARMVS PAIKPL SLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK
Sbjct: 61  SALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120

Query: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY 180
           IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEAR VGFSEENGTLGDIY
Sbjct: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIY 180

Query: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKN SVI
Sbjct: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNISVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300
           AVCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWS+ALGSPFTFATT
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSIALGSPFTFATT 300

Query: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360
           LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAE+LAYKNTVESITGIIS+TISTK
Sbjct: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISRTISTK 360

Query: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYNSFSPDEKKEFERAYSAS+YPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS
Sbjct: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540

Query: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           SA+NQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN
Sbjct: 541 SAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 588

BLAST of CmaCh19G010300 vs. ExPASy TrEMBL
Match: A0A0A0K5T7 (Ketol-acid reductoisomerase OS=Cucumis sativus OX=3659 GN=Csa_7G051370 PE=3 SV=1)

HSP 1 Score: 1102.0 bits (2849), Expect = 0.0e+00
Identity = 560/588 (95.24%), Postives = 572/588 (97.28%), Query Frame = 0

Query: 2   AAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVGS 61
           AA T+F+PSISAS +KTLK AA+LHPL PSSL FLSSS  SSG  ALKAVG+  AN +GS
Sbjct: 3   AATTHFAPSISASSAKTLKHAAALHPLAPSSLSFLSSS--SSGLNALKAVGISAANGIGS 62

Query: 62  ALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKKI 121
           ALGARMVS PAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKKI
Sbjct: 63  ALGARMVSVPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKKI 122

Query: 122 GVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIYE 181
           GVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEAR VGFSEENGTLGDIYE
Sbjct: 123 GVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIYE 182

Query: 182 TISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIA 241
           TISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GLDFPKN SVIA
Sbjct: 183 TISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLDFPKNISVIA 242

Query: 242 VCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATTL 301
           VCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWS+ALGSPFTFATTL
Sbjct: 243 VCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSIALGSPFTFATTL 302

Query: 302 EQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTKG 361
           EQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAE+LAYKNTVESITGIIS+TISTKG
Sbjct: 303 EQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISRTISTKG 362

Query: 362 MLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGL 421
           MLAVYNSFSPDEKKEFERAYSAS+YPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGL
Sbjct: 363 MLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGL 422

Query: 422 PAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSE 481
           PAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSE
Sbjct: 423 PAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSE 482

Query: 482 IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNGS 541
           IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNGS
Sbjct: 483 IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNGS 542

Query: 542 AVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSS 590
           AVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQS+
Sbjct: 543 AVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSN 588

BLAST of CmaCh19G010300 vs. NCBI nr
Match: XP_022969037.1 (ketol-acid reductoisomerase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 590/590 (100.00%), Postives = 590/590 (100.00%), Query Frame = 0

Query: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60
           MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60

Query: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120
           SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120

Query: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY 180
           IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY
Sbjct: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY 180

Query: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI
Sbjct: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300
           AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300

Query: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360
           LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK
Sbjct: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360

Query: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS
Sbjct: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540

Query: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN
Sbjct: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of CmaCh19G010300 vs. NCBI nr
Match: XP_022952496.1 (ketol-acid reductoisomerase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 586/590 (99.32%), Postives = 587/590 (99.49%), Query Frame = 0

Query: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60
           MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSG FALKAVGMGG NAVG
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGSFALKAVGMGGGNAVG 60

Query: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120
           SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIK+
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ 120

Query: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY 180
           IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEAR VGFSEENGTLGDIY
Sbjct: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIY 180

Query: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI
Sbjct: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300
           AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300

Query: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360
           LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK
Sbjct: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360

Query: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS
Sbjct: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540

Query: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN
Sbjct: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of CmaCh19G010300 vs. NCBI nr
Match: XP_023554080.1 (ketol-acid reductoisomerase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 585/590 (99.15%), Postives = 587/590 (99.49%), Query Frame = 0

Query: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60
           MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSG FALKAVGMGG NAVG
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGSFALKAVGMGGGNAVG 60

Query: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120
           SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIK+
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ 120

Query: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY 180
           IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEAR VGFSEENGTLGDIY
Sbjct: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIY 180

Query: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI
Sbjct: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300
           AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300

Query: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360
           LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK
Sbjct: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360

Query: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVA+GSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVATGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS
Sbjct: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540

Query: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN
Sbjct: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of CmaCh19G010300 vs. NCBI nr
Match: KAG6572402.1 (Ketol-acid reductoisomerase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1144.0 bits (2958), Expect = 0.0e+00
Identity = 583/590 (98.81%), Postives = 585/590 (99.15%), Query Frame = 0

Query: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60
           MAAATYFSPSISASLSKTLKQAASLHP TPSSLGFLSSSSA SG FALKAVGMGG NAVG
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPFTPSSLGFLSSSSAFSGSFALKAVGMGGGNAVG 60

Query: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120
           SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIK+
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ 120

Query: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY 180
           IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEAR VGFSEENGTLGDIY
Sbjct: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIY 180

Query: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI
Sbjct: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300
           AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300

Query: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360
           LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK
Sbjct: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360

Query: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVA+GSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVATGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS
Sbjct: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540

Query: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN
Sbjct: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of CmaCh19G010300 vs. NCBI nr
Match: XP_038888114.1 (ketol-acid reductoisomerase, chloroplastic [Benincasa hispida])

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 571/590 (96.78%), Postives = 579/590 (98.14%), Query Frame = 0

Query: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60
           MAAATYF+PSIS S +KTLKQAA+L PLTPSSLGFLSSSS SSG  ALKAVGMGGAN VG
Sbjct: 1   MAAATYFAPSISVSSTKTLKQAAALQPLTPSSLGFLSSSS-SSGSNALKAVGMGGANGVG 60

Query: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120
           SALGARMVS PAIKPL SLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK
Sbjct: 61  SALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120

Query: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY 180
           IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEAR VGFSEENGTLGDIY
Sbjct: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIY 180

Query: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKN SVI
Sbjct: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNISVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300
           AVCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300

Query: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360
           LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK
Sbjct: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360

Query: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYNSFSPDEKKEFERAYSAS+YPCMDILYECYEDVA+GSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVATGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS
Sbjct: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540

Query: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           SA+NQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQS+N
Sbjct: 541 SAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSNN 589

BLAST of CmaCh19G010300 vs. TAIR 10
Match: AT3G58610.1 (ketol-acid reductoisomerase )

HSP 1 Score: 977.2 bits (2525), Expect = 5.7e-285
Identity = 503/595 (84.54%), Postives = 540/595 (90.76%), Query Frame = 0

Query: 1   MAAAT-YFSPSIS----ASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGG 60
           MAAAT   +PS+S    +S SKTL  ++    L   ++GFLSSSS S         G G 
Sbjct: 1   MAAATSSIAPSLSCPSPSSSSKTL-WSSKARTLALPNIGFLSSSSKSLRSLTATVAGNG- 60

Query: 61  ANAVGSALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAF 120
             A GS+L ARMVS+ A+K  +SLDF+TSVFKKEKV+LAG++EYIVRGGRDLF  LP+AF
Sbjct: 61  --ATGSSLAARMVSSSAVKAPVSLDFETSVFKKEKVSLAGYEEYIVRGGRDLFKHLPDAF 120

Query: 121 KGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGT 180
           KGIK+IGVIGWGSQ PAQAQNLRDSL EAKSDIVVKIGLRKGSRSF EAR  GF+EE+GT
Sbjct: 121 KGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKIGLRKGSRSFEEARAAGFTEESGT 180

Query: 181 LGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPK 240
           LGDI+ETI+ SDLV+LLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GLDFPK
Sbjct: 181 LGDIWETIAGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLDFPK 240

Query: 241 NFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPF 300
           N SV+AVCPKGMGPSVRRLYVQGKEINGAGIN+SFAVHQDVDGRA DVALGWSVALGSPF
Sbjct: 241 NISVVAVCPKGMGPSVRRLYVQGKEINGAGINASFAVHQDVDGRAADVALGWSVALGSPF 300

Query: 301 TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISK 360
           TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+EDLAYKNTVE ITG IS+
Sbjct: 301 TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVECITGTISR 360

Query: 361 TISTKGMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRF 420
           TIST+GMLAVYNS S + KK+FE AYSASFYPCM+ILYECYEDV SGSEIRSVVLAGRRF
Sbjct: 361 TISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGSEIRSVVLAGRRF 420

Query: 421 YEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKK 480
           YEKEGLPAFPMGNIDQTRMWKVGERVR+SRPAGDLGPLYPFTAGVYVALMMAQIEILRKK
Sbjct: 421 YEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKK 480

Query: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV 540
           GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV
Sbjct: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV 540

Query: 541 AIDNGSAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           A+D+G+A+N+DLISNF SDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN
Sbjct: 541 AVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591

BLAST of CmaCh19G010300 vs. TAIR 10
Match: AT3G58610.2 (ketol-acid reductoisomerase )

HSP 1 Score: 977.2 bits (2525), Expect = 5.7e-285
Identity = 503/595 (84.54%), Postives = 540/595 (90.76%), Query Frame = 0

Query: 1   MAAAT-YFSPSIS----ASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGG 60
           MAAAT   +PS+S    +S SKTL  ++    L   ++GFLSSSS S         G G 
Sbjct: 1   MAAATSSIAPSLSCPSPSSSSKTL-WSSKARTLALPNIGFLSSSSKSLRSLTATVAGNG- 60

Query: 61  ANAVGSALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAF 120
             A GS+L ARMVS+ A+K  +SLDF+TSVFKKEKV+LAG++EYIVRGGRDLF  LP+AF
Sbjct: 61  --ATGSSLAARMVSSSAVKAPVSLDFETSVFKKEKVSLAGYEEYIVRGGRDLFKHLPDAF 120

Query: 121 KGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGT 180
           KGIK+IGVIGWGSQ PAQAQNLRDSL EAKSDIVVKIGLRKGSRSF EAR  GF+EE+GT
Sbjct: 121 KGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKIGLRKGSRSFEEARAAGFTEESGT 180

Query: 181 LGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPK 240
           LGDI+ETI+ SDLV+LLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GLDFPK
Sbjct: 181 LGDIWETIAGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLDFPK 240

Query: 241 NFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPF 300
           N SV+AVCPKGMGPSVRRLYVQGKEINGAGIN+SFAVHQDVDGRA DVALGWSVALGSPF
Sbjct: 241 NISVVAVCPKGMGPSVRRLYVQGKEINGAGINASFAVHQDVDGRAADVALGWSVALGSPF 300

Query: 301 TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISK 360
           TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+EDLAYKNTVE ITG IS+
Sbjct: 301 TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVECITGTISR 360

Query: 361 TISTKGMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRF 420
           TIST+GMLAVYNS S + KK+FE AYSASFYPCM+ILYECYEDV SGSEIRSVVLAGRRF
Sbjct: 361 TISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGSEIRSVVLAGRRF 420

Query: 421 YEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKK 480
           YEKEGLPAFPMGNIDQTRMWKVGERVR+SRPAGDLGPLYPFTAGVYVALMMAQIEILRKK
Sbjct: 421 YEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKK 480

Query: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV 540
           GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV
Sbjct: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV 540

Query: 541 AIDNGSAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           A+D+G+A+N+DLISNF SDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN
Sbjct: 541 AVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591

BLAST of CmaCh19G010300 vs. TAIR 10
Match: AT3G58610.3 (ketol-acid reductoisomerase )

HSP 1 Score: 977.2 bits (2525), Expect = 5.7e-285
Identity = 503/595 (84.54%), Postives = 540/595 (90.76%), Query Frame = 0

Query: 1   MAAAT-YFSPSIS----ASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGG 60
           MAAAT   +PS+S    +S SKTL  ++    L   ++GFLSSSS S         G G 
Sbjct: 1   MAAATSSIAPSLSCPSPSSSSKTL-WSSKARTLALPNIGFLSSSSKSLRSLTATVAGNG- 60

Query: 61  ANAVGSALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAF 120
             A GS+L ARMVS+ A+K  +SLDF+TSVFKKEKV+LAG++EYIVRGGRDLF  LP+AF
Sbjct: 61  --ATGSSLAARMVSSSAVKAPVSLDFETSVFKKEKVSLAGYEEYIVRGGRDLFKHLPDAF 120

Query: 121 KGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGT 180
           KGIK+IGVIGWGSQ PAQAQNLRDSL EAKSDIVVKIGLRKGSRSF EAR  GF+EE+GT
Sbjct: 121 KGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKIGLRKGSRSFEEARAAGFTEESGT 180

Query: 181 LGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPK 240
           LGDI+ETI+ SDLV+LLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GLDFPK
Sbjct: 181 LGDIWETIAGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLDFPK 240

Query: 241 NFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPF 300
           N SV+AVCPKGMGPSVRRLYVQGKEINGAGIN+SFAVHQDVDGRA DVALGWSVALGSPF
Sbjct: 241 NISVVAVCPKGMGPSVRRLYVQGKEINGAGINASFAVHQDVDGRAADVALGWSVALGSPF 300

Query: 301 TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISK 360
           TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+EDLAYKNTVE ITG IS+
Sbjct: 301 TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVECITGTISR 360

Query: 361 TISTKGMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRF 420
           TIST+GMLAVYNS S + KK+FE AYSASFYPCM+ILYECYEDV SGSEIRSVVLAGRRF
Sbjct: 361 TISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGSEIRSVVLAGRRF 420

Query: 421 YEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKK 480
           YEKEGLPAFPMGNIDQTRMWKVGERVR+SRPAGDLGPLYPFTAGVYVALMMAQIEILRKK
Sbjct: 421 YEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKK 480

Query: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV 540
           GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV
Sbjct: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV 540

Query: 541 AIDNGSAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591
           A+D+G+A+N+DLISNF SDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN
Sbjct: 541 AVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q057588.0e-28484.54Ketol-acid reductoisomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q012921.6e-27983.33Ketol-acid reductoisomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=AHRI ... [more]
O820432.7e-27982.88Ketol-acid reductoisomerase, chloroplastic OS=Pisum sativum OX=3888 GN=PGAAIR PE... [more]
Q65XK02.7e-27180.85Ketol-acid reductoisomerase, chloroplastic OS=Oryza sativa subsp. japonica OX=39... [more]
P386747.5e-4030.25Ketol-acid reductoisomerase, mitochondrial OS=Neurospora crassa (strain ATCC 246... [more]
Match NameE-valueIdentityDescription
A0A6J1HV870.0e+00100.00Ketol-acid reductoisomerase OS=Cucurbita maxima OX=3661 GN=LOC111468154 PE=3 SV=... [more]
A0A6J1GKD70.0e+0099.32Ketol-acid reductoisomerase OS=Cucurbita moschata OX=3662 GN=LOC111455167 PE=3 S... [more]
A0A5D3C5630.0e+0095.93Ketol-acid reductoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3C0390.0e+0095.93Ketol-acid reductoisomerase OS=Cucumis melo OX=3656 GN=LOC103495472 PE=3 SV=1[more]
A0A0A0K5T70.0e+0095.24Ketol-acid reductoisomerase OS=Cucumis sativus OX=3659 GN=Csa_7G051370 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022969037.10.0e+00100.00ketol-acid reductoisomerase, chloroplastic-like [Cucurbita maxima][more]
XP_022952496.10.0e+0099.32ketol-acid reductoisomerase, chloroplastic-like [Cucurbita moschata][more]
XP_023554080.10.0e+0099.15ketol-acid reductoisomerase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG6572402.10.0e+0098.81Ketol-acid reductoisomerase, chloroplastic, partial [Cucurbita argyrosperma subs... [more]
XP_038888114.10.0e+0096.78ketol-acid reductoisomerase, chloroplastic [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G58610.15.7e-28584.54ketol-acid reductoisomerase [more]
AT3G58610.25.7e-28584.54ketol-acid reductoisomerase [more]
AT3G58610.35.7e-28584.54ketol-acid reductoisomerase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 295..568
e-value: 1.3E-156
score: 522.1
IPR000506Ketol-acid reductoisomerase, C-terminalPFAMPF01450IlvCcoord: 302..446
e-value: 4.9E-33
score: 114.5
coord: 459..528
e-value: 1.4E-7
score: 31.9
IPR000506Ketol-acid reductoisomerase, C-terminalPROSITEPS51851KARI_Ccoord: 449..585
score: 23.143694
IPR000506Ketol-acid reductoisomerase, C-terminalPROSITEPS51851KARI_Ccoord: 300..448
score: 32.230827
IPR016206Ketol-acid reductoisomerase, plantPIRSFPIRSF000118Ilv5_plantcoord: 1..589
e-value: 0.0
score: 1220.7
IPR013116Ketol-acid reductoisomerase, N-terminalPFAMPF07991IlvNcoord: 119..293
e-value: 1.6E-30
score: 105.9
IPR013116Ketol-acid reductoisomerase, N-terminalPROSITEPS51850KARI_Ncoord: 101..299
score: 50.268559
NoneNo IPR availableGENE3D3.40.50.720coord: 67..294
e-value: 5.6E-109
score: 364.6
NoneNo IPR availablePANTHERPTHR21371:SF20KETOL-ACID REDUCTOISOMERASEcoord: 3..589
IPR013023Ketol-acid reductoisomerasePANTHERPTHR21371KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIALcoord: 3..589
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 302..587
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 79..297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh19G010300.1CmaCh19G010300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009097 isoleucine biosynthetic process
biological_process GO:0009099 valine biosynthetic process
biological_process GO:0009082 branched-chain amino acid biosynthetic process
molecular_function GO:0016853 isomerase activity
molecular_function GO:0004455 ketol-acid reductoisomerase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity