CmaCh19G003520 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh19G003520
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	polygalacturonase-like
Location	Cma_Chr19: 3741534 .. 3742250 (+)
RNA-Seq Expression	CmaCh19G003520
Synteny	CmaCh19G003520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCTGTGACTCATCTGAACCAGCCACTATTTACGTTCCCGGGAGTATATTCTTCGTTCGAAGTGTGAGATTTAATGGGCCTAGTAAGAACAATGCTATTACTGTTCTCATTGATGGAACTCTTGTGGCTTCCTCAAACATTCAAGTTTTAGCTAATTCTAGTTCTTGGATTTTGTTTAATCACATAAATGGGTTTTCCATTACTGGCGGTATTATTGATGGGCAAGGAACTCTCTTGTGGAATTGTAAACACTCAGGGAAGAATTGCCCCATTGGAGCTCGGGTATGCAGAATTTGAGTTGGCTCCATATAAAAAAATTTGAAATTTTTTCTTTAACTCAAAGTTGAATGAAATGACCAGATTAATTACAATATTATTTTAAATTTTTACTAATTCTGGCTTTTGATTGTTTTGAATTGATAGGGTCTTCAAATAAATGATGGAAAGAATGTGAACATCAATGGATTATCTTCAATTAACAGCCAATTGTTCAGCATTGTAATGAATCGGTGCCAAAACGTGCATATGACAAGAGTGAATGTCTTGGCTGCATGCGATAGTCCAAACACTGATGGCATCCATGTGCGACAATCATCAGATGTGACCATCCTCAACTCAAACATTAGAAACGGTGACGACTGCATCTCCATCGGTCCAGGCACATCCAACATGTGGATCGAAAGTATTACATGTGGACCTGGACATGGAATCATGTAA

mRNA sequence

GCCTGTGACTCATCTGAACCAGCCACTATTTACGTTCCCGGGAGTATATTCTTCGTTCGAAGTGTGAGATTTAATGGGCCTAGTAAGAACAATGCTATTACTGTTCTCATTGATGGAACTCTTGTGGCTTCCTCAAACATTCAAGTTTTAGCTAATTCTAGTTCTTGGATTTTGTTTAATCACATAAATGGGTTTTCCATTACTGGCGGTATTATTGATGGGCAAGGAACTCTCTTGTGGAATTGTAAACACTCAGGGAAGAATTGCCCCATTGGAGCTCGGGGTCTTCAAATAAATGATGGAAAGAATGTGAACATCAATGGATTATCTTCAATTAACAGCCAATTGTTCAGCATTGTAATGAATCGGTGCCAAAACGTGCATATGACAAGAGTGAATGTCTTGGCTGCATGCGATAGTCCAAACACTGATGGCATCCATGTGCGACAATCATCAGATGTGACCATCCTCAACTCAAACATTAGAAACGGTGACGACTGCATCTCCATCGGTCCAGGCACATCCAACATGTGGATCGAAAGTATTACATGTGGACCTGGACATGGAATCATGTAA

Coding sequence (CDS)

GCCTGTGACTCATCTGAACCAGCCACTATTTACGTTCCCGGGAGTATATTCTTCGTTCGAAGTGTGAGATTTAATGGGCCTAGTAAGAACAATGCTATTACTGTTCTCATTGATGGAACTCTTGTGGCTTCCTCAAACATTCAAGTTTTAGCTAATTCTAGTTCTTGGATTTTGTTTAATCACATAAATGGGTTTTCCATTACTGGCGGTATTATTGATGGGCAAGGAACTCTCTTGTGGAATTGTAAACACTCAGGGAAGAATTGCCCCATTGGAGCTCGGGGTCTTCAAATAAATGATGGAAAGAATGTGAACATCAATGGATTATCTTCAATTAACAGCCAATTGTTCAGCATTGTAATGAATCGGTGCCAAAACGTGCATATGACAAGAGTGAATGTCTTGGCTGCATGCGATAGTCCAAACACTGATGGCATCCATGTGCGACAATCATCAGATGTGACCATCCTCAACTCAAACATTAGAAACGGTGACGACTGCATCTCCATCGGTCCAGGCACATCCAACATGTGGATCGAAAGTATTACATGTGGACCTGGACATGGAATCATGTAA

Protein sequence

ACDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFNHINGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSIVMNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWIESITCGPGHGIM

Homology

[Close]

BLAST of CmaCh19G003520 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.5e-61
Identity = 113/191 (59.16%), Postives = 142/191 (74.35%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFN 60
           AC S  P  IYVP   FF+R V F+GP KNNAIT  I GTLVA S+ +V+ N+++WI F+
Sbjct: 54  ACASMNPGVIYVPAGTFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFH 113

Query: 61  HINGFSITGGIIDGQGTLLWNCKH-SGKNCPIGARGLQINDGKNVNINGLSSINSQLFSI 120
           H+NG +I+GGI+DGQGT LW CK   G++CP GA  L  +D  N+ ++GL+S+NSQ+F I
Sbjct: 114 HVNGVTISGGILDGQGTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHI 173

Query: 121 VMNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWI 180
           V+N  QNV M  V V  + +SPNTDGIHV+ SS VTILNS I  GDDC+SIGPGTSN+WI
Sbjct: 174 VINDFQNVQMQGVRVSRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWI 233

Query: 181 ESITCGPGHGI 191
           E + CGPGHGI
Sbjct: 234 EGVACGPGHGI 244

[Close]

BLAST of CmaCh19G003520 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 5.1e-46
Identity = 95/192 (49.48%), Postives = 128/192 (66.67%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNG-PSKNNAITVLIDGTLVASSNIQVLANSSSWILF 60
           AC S+ P TI VP   F V ++ F+G   K   I++ I G++VA  + +++A+S  WI F
Sbjct: 59  ACASANPTTIIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWF 118

Query: 61  NHINGFSITGGIIDGQGTLLWNCKHS-GKNCPIGARGLQINDGKNVNINGLSSINSQLFS 120
             +   SI GGI+D QGT LW CK++ G NCP GA+ L  +   N+ I+GL+SINSQ F 
Sbjct: 119 EDVTDVSIYGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFH 178

Query: 121 IVMNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMW 180
           IV++   NV++  V V A  +SPNTDGIHV  S  V I NS I  GDDCISIGPG++N++
Sbjct: 179 IVIDNSNNVNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVF 238

Query: 181 IESITCGPGHGI 191
           I++I CGPGHGI
Sbjct: 239 IQTIRCGPGHGI 250

[Close]

BLAST of CmaCh19G003520 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.2e-34
Identity = 78/191 (40.84%), Postives = 112/191 (58.64%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFN 60
           AC S+ P+TI +P   F +  V+  GP K + + + I  TL A S+   L     W+  N
Sbjct: 51  ACQSTSPSTIVIPKGTFTMNQVKLEGPCK-SPLELQIQATLKAPSDPSQL-KVGEWLTVN 110

Query: 61  HINGFSIT-GGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSI 120
            ++ F+++ GGI+DGQ    W CK S K C      L  N   N  I  +++++S+ F +
Sbjct: 111 KLDQFTMSGGGILDGQAAAAWECKQS-KKCNKLPNNLSFNSLTNSTIKDITTLDSKSFHV 170

Query: 121 VMNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWI 180
            +N+C+N+   R NV A  +SPNTDGIHV +SS V I +SN   GDDCIS+G  T  ++I
Sbjct: 171 NVNQCKNLTFIRFNVSAPANSPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYI 230

Query: 181 ESITCGPGHGI 191
             +TCGPGHGI
Sbjct: 231 TRVTCGPGHGI 238

[Close]

BLAST of CmaCh19G003520 vs. ExPASy Swiss-Prot
Match: P49062 (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.0e-34
Identity = 73/192 (38.02%), Postives = 117/192 (60.94%), Query Frame = 0


Query: 2   CDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFNH 61
           CDS  PAT+ VP   F    V F GP K+  +TV + GT++A+++      +  W LF  
Sbjct: 79  CDSPVPATLLVPKGTFLAGPVIFAGPCKSK-VTVNVIGTIIATTSGYA---TPEWFLFER 138

Query: 62  INGFSITG-GIIDGQGTLLWNCKHSGK--NCPIGARGLQINDGKNVNINGLSSINSQLFS 121
           ++   +TG G   G+G  +W     GK   C +    L+  + KNV ING+SS+N++ F 
Sbjct: 139 VDNLVLTGTGTFHGKGEAVWKADGCGKKVQCNLPPTSLKFRNMKNVEINGISSVNAKAFH 198

Query: 122 IVMNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMW 181
           + + + +NV++  + + A  +SPNTDGIH+  + +V+IL+S I  GDDC+S+G G++N+ 
Sbjct: 199 MFLVKTENVNIQNIKLTAPAESPNTDGIHLSNADNVSILDSTIATGDDCVSVGRGSNNVT 258

Query: 182 IESITCGPGHGI 191
           +E + CGPGHG+
Sbjct: 259 VERVICGPGHGL 266

[Close]

BLAST of CmaCh19G003520 vs. ExPASy Swiss-Prot
Match: P26216 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.7e-33
Identity = 78/193 (40.41%), Postives = 116/193 (60.10%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFN 60
           AC  +   TI +P   F V  + F GP K + +T+ +DG L+A++++    +  +WI   
Sbjct: 67  ACGGTGKQTILIPKGDFLVGQLNFTGPCKGD-VTIQVDGNLLATTDLSQYKDHGNWIEIL 126

Query: 61  HINGFSITG-GIIDGQGTLLWNCKHSGK--NCPIGARGLQINDGKNVNINGLSSINSQLF 120
            ++   ITG G +DGQG  +W+     K  +C I    L ++   N  ++G++ +NS+ F
Sbjct: 127 RVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSGVTLLNSKFF 186

Query: 121 SIVMNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNM 180
            + M RC+++ +  V V A  DSPNTDGIH+  SS +TI N+ I  GDDCISIGPGTS +
Sbjct: 187 HMNMYRCKDMLIKDVTVTAPGDSPNTDGIHMGDSSGITITNTVIGVGDDCISIGPGTSKV 246

Query: 181 WIESITCGPGHGI 191
            I  +TCGPGHGI
Sbjct: 247 NITGVTCGPGHGI 258

[Close]

BLAST of CmaCh19G003520 vs. ExPASy TrEMBL
Match: A0A6J1IEV2 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111473552 PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.5e-96
Identity = 178/190 (93.68%), Postives = 181/190 (95.26%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFN 60
           ACDSSEPATIYVP  IFFVRSVRFNGP KNNAIT+LIDGTLVASS+IQVLANSSSWILFN
Sbjct: 63  ACDSSEPATIYVPEGIFFVRSVRFNGPCKNNAITILIDGTLVASSDIQVLANSSSWILFN 122

Query: 61  HINGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSIV 120
           HING SITGGIIDGQGT LWNCKHS K+CPIGAR LQINDGKNVNINGLSSINSQLFSIV
Sbjct: 123 HINGLSITGGIIDGQGTPLWNCKHSRKSCPIGARSLQINDGKNVNINGLSSINSQLFSIV 182

Query: 121 MNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWIE 180
           MNRCQNVHMTRVNVLAA DSPNTDGIHVRQSSDVTILNSNI NGDDCISIGPGTSNMWIE
Sbjct: 183 MNRCQNVHMTRVNVLAAGDSPNTDGIHVRQSSDVTILNSNIGNGDDCISIGPGTSNMWIE 242

Query: 181 SITCGPGHGI 191
           SITCGPGHGI
Sbjct: 243 SITCGPGHGI 252

[Close]

BLAST of CmaCh19G003520 vs. ExPASy TrEMBL
Match: A0A6J1F489 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111440267 PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 6.1e-95
Identity = 175/190 (92.11%), Postives = 179/190 (94.21%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFN 60
           ACDSSEPATIYVP  IFFVRSVRFNGP KNNAITVLIDGTLVASS+I VLANSSSWILFN
Sbjct: 63  ACDSSEPATIYVPEGIFFVRSVRFNGPCKNNAITVLIDGTLVASSDIHVLANSSSWILFN 122

Query: 61  HINGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSIV 120
           HING SITGGIIDGQGT LWNCKHSGK+CPIGAR LQINDGKNVNINGLSSINSQLF I 
Sbjct: 123 HINGLSITGGIIDGQGTPLWNCKHSGKSCPIGARSLQINDGKNVNINGLSSINSQLFGIG 182

Query: 121 MNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWIE 180
           MNRCQNVHMTRVNVLAA DSPNTDGIHV+QSSDVTILNSNI NGDDCISIGPGTSNMWIE
Sbjct: 183 MNRCQNVHMTRVNVLAAGDSPNTDGIHVQQSSDVTILNSNIGNGDDCISIGPGTSNMWIE 242

Query: 181 SITCGPGHGI 191
           +ITCGPGHGI
Sbjct: 243 TITCGPGHGI 252

[Close]

BLAST of CmaCh19G003520 vs. ExPASy TrEMBL
Match: A0A6J1HFX3 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111463942 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 8.8e-94
Identity = 173/189 (91.53%), Postives = 178/189 (94.18%), Query Frame = 0


Query: 2   CDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFNH 61
           CDSSEPATIYVP  IFFVRSVRFNGP KNNAITVLIDGTLVASS+IQVLANSSSWI FNH
Sbjct: 64  CDSSEPATIYVPEGIFFVRSVRFNGPCKNNAITVLIDGTLVASSDIQVLANSSSWISFNH 123

Query: 62  INGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSIVM 121
           ING SITGGIIDGQGT LWNCKHS K+CPIGAR LQINDGKNVNINGLSSINSQLF IVM
Sbjct: 124 INGLSITGGIIDGQGTPLWNCKHSRKSCPIGARSLQINDGKNVNINGLSSINSQLFGIVM 183

Query: 122 NRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWIES 181
           NRCQNVHM+RVNVLAA DSPNTDGIHV+QSSDVTILNSNI NGDDCISIGPGTSNMWIE+
Sbjct: 184 NRCQNVHMSRVNVLAAGDSPNTDGIHVQQSSDVTILNSNIGNGDDCISIGPGTSNMWIET 243

Query: 182 ITCGPGHGI 191
           ITCGPGHGI
Sbjct: 244 ITCGPGHGI 252

[Close]

BLAST of CmaCh19G003520 vs. ExPASy TrEMBL
Match: A0A6J1I843 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111471802 PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 8.3e-84
Identity = 154/190 (81.05%), Postives = 169/190 (88.95%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFN 60
           ACDSS PATIYVP  IF+VRSV FNGP KNN ITVLIDGTLVASS+IQVLA + SWI F+
Sbjct: 66  ACDSSTPATIYVPDGIFYVRSVSFNGPCKNNVITVLIDGTLVASSDIQVLAKTKSWIEFS 125

Query: 61  HINGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSIV 120
           HI+G SITGG IDGQGT LWNCKHSGK+CP+GA  LQIND +NVNINGLSSINSQ F+IV
Sbjct: 126 HIDGLSITGGTIDGQGTALWNCKHSGKSCPVGATSLQINDAQNVNINGLSSINSQKFNIV 185

Query: 121 MNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWIE 180
           ++ CQNVHMTRVNV AA DSPNTDGIHV+QSSDVTILNS+I NGDDCISI PGTSN+W+E
Sbjct: 186 VHGCQNVHMTRVNVSAADDSPNTDGIHVQQSSDVTILNSSISNGDDCISISPGTSNLWME 245

Query: 181 SITCGPGHGI 191
           SITCGPGHGI
Sbjct: 246 SITCGPGHGI 255

[Close]

BLAST of CmaCh19G003520 vs. ExPASy TrEMBL
Match: A0A6J1IF00 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472165 PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 8.3e-84
Identity = 154/190 (81.05%), Postives = 169/190 (88.95%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFN 60
           ACDSS PATIYVP  IF+VRSV FNGP KNN ITVLIDGTLVASS+IQVLA + SWI F+
Sbjct: 66  ACDSSTPATIYVPDGIFYVRSVSFNGPCKNNVITVLIDGTLVASSDIQVLAKTKSWIEFS 125

Query: 61  HINGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSIV 120
           HI+G SITGG IDGQGT LWNCKHSGK+CP+GA  LQIND +NVNINGLSSINSQ F+IV
Sbjct: 126 HIDGLSITGGTIDGQGTALWNCKHSGKSCPVGATSLQINDAQNVNINGLSSINSQKFNIV 185

Query: 121 MNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWIE 180
           ++ CQNVHMTRVNV AA DSPNTDGIHV+QSSDVTILNS+I NGDDCISI PGTSN+W+E
Sbjct: 186 VHGCQNVHMTRVNVSAADDSPNTDGIHVQQSSDVTILNSSISNGDDCISISPGTSNLWME 245

Query: 181 SITCGPGHGI 191
           SITCGPGHGI
Sbjct: 246 SITCGPGHGI 255

[Close]

BLAST of CmaCh19G003520 vs. NCBI nr
Match: XP_022974795.1 (polygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 362.1 bits (928), Expect = 3.0e-96
Identity = 178/190 (93.68%), Postives = 181/190 (95.26%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFN 60
           ACDSSEPATIYVP  IFFVRSVRFNGP KNNAIT+LIDGTLVASS+IQVLANSSSWILFN
Sbjct: 63  ACDSSEPATIYVPEGIFFVRSVRFNGPCKNNAITILIDGTLVASSDIQVLANSSSWILFN 122

Query: 61  HINGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSIV 120
           HING SITGGIIDGQGT LWNCKHS K+CPIGAR LQINDGKNVNINGLSSINSQLFSIV
Sbjct: 123 HINGLSITGGIIDGQGTPLWNCKHSRKSCPIGARSLQINDGKNVNINGLSSINSQLFSIV 182

Query: 121 MNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWIE 180
           MNRCQNVHMTRVNVLAA DSPNTDGIHVRQSSDVTILNSNI NGDDCISIGPGTSNMWIE
Sbjct: 183 MNRCQNVHMTRVNVLAAGDSPNTDGIHVRQSSDVTILNSNIGNGDDCISIGPGTSNMWIE 242

Query: 181 SITCGPGHGI 191
           SITCGPGHGI
Sbjct: 243 SITCGPGHGI 252

[Close]

BLAST of CmaCh19G003520 vs. NCBI nr
Match: XP_022933258.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 356.7 bits (914), Expect = 1.3e-94
Identity = 175/190 (92.11%), Postives = 179/190 (94.21%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFN 60
           ACDSSEPATIYVP  IFFVRSVRFNGP KNNAITVLIDGTLVASS+I VLANSSSWILFN
Sbjct: 63  ACDSSEPATIYVPEGIFFVRSVRFNGPCKNNAITVLIDGTLVASSDIHVLANSSSWILFN 122

Query: 61  HINGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSIV 120
           HING SITGGIIDGQGT LWNCKHSGK+CPIGAR LQINDGKNVNINGLSSINSQLF I 
Sbjct: 123 HINGLSITGGIIDGQGTPLWNCKHSGKSCPIGARSLQINDGKNVNINGLSSINSQLFGIG 182

Query: 121 MNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWIE 180
           MNRCQNVHMTRVNVLAA DSPNTDGIHV+QSSDVTILNSNI NGDDCISIGPGTSNMWIE
Sbjct: 183 MNRCQNVHMTRVNVLAAGDSPNTDGIHVQQSSDVTILNSNIGNGDDCISIGPGTSNMWIE 242

Query: 181 SITCGPGHGI 191
           +ITCGPGHGI
Sbjct: 243 TITCGPGHGI 252

[Close]

BLAST of CmaCh19G003520 vs. NCBI nr
Match: XP_022963687.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 352.8 bits (904), Expect = 1.8e-93
Identity = 173/189 (91.53%), Postives = 178/189 (94.18%), Query Frame = 0


Query: 2   CDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFNH 61
           CDSSEPATIYVP  IFFVRSVRFNGP KNNAITVLIDGTLVASS+IQVLANSSSWI FNH
Sbjct: 64  CDSSEPATIYVPEGIFFVRSVRFNGPCKNNAITVLIDGTLVASSDIQVLANSSSWISFNH 123

Query: 62  INGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSIVM 121
           ING SITGGIIDGQGT LWNCKHS K+CPIGAR LQINDGKNVNINGLSSINSQLF IVM
Sbjct: 124 INGLSITGGIIDGQGTPLWNCKHSRKSCPIGARSLQINDGKNVNINGLSSINSQLFGIVM 183

Query: 122 NRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWIES 181
           NRCQNVHM+RVNVLAA DSPNTDGIHV+QSSDVTILNSNI NGDDCISIGPGTSNMWIE+
Sbjct: 184 NRCQNVHMSRVNVLAAGDSPNTDGIHVQQSSDVTILNSNIGNGDDCISIGPGTSNMWIET 243

Query: 182 ITCGPGHGI 191
           ITCGPGHGI
Sbjct: 244 ITCGPGHGI 252

[Close]

BLAST of CmaCh19G003520 vs. NCBI nr
Match: XP_023521278.1 (polygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 330.1 bits (845), Expect = 1.3e-86
Identity = 167/190 (87.89%), Postives = 171/190 (90.00%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFN 60
           ACDSSEPATIYVP  IFFV SVRFNGP KNNAIT+LIDGTLVASS+IQVLANSSSWILFN
Sbjct: 35  ACDSSEPATIYVPEGIFFVLSVRFNGPCKNNAITILIDGTLVASSDIQVLANSSSWILFN 94

Query: 61  HINGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSIV 120
           HING SITGGIIDGQGT LWNCK SGK+CPIGAR  QINDGKNVNINGLSSINSQL  IV
Sbjct: 95  HINGLSITGGIIDGQGTALWNCKRSGKSCPIGARSFQINDGKNVNINGLSSINSQLSGIV 154

Query: 121 MNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWIE 180
           MNRCQNVHMTRVNVLAA DSPNT+GIHV Q SDVTILNSNI NGDDCISI P TSNMWIE
Sbjct: 155 MNRCQNVHMTRVNVLAAGDSPNTNGIHV-QISDVTILNSNIGNGDDCISISPETSNMWIE 214

Query: 181 SITCGPGHGI 191
           SIT GP HGI
Sbjct: 215 SITRGPRHGI 223

[Close]

BLAST of CmaCh19G003520 vs. NCBI nr
Match: XP_022973584.1 (polygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 319.7 bits (818), Expect = 1.7e-83
Identity = 154/190 (81.05%), Postives = 169/190 (88.95%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFN 60
           ACDSS PATIYVP  IF+VRSV FNGP KNN ITVLIDGTLVASS+IQVLA + SWI F+
Sbjct: 66  ACDSSTPATIYVPDGIFYVRSVSFNGPCKNNVITVLIDGTLVASSDIQVLAKTKSWIEFS 125

Query: 61  HINGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSIV 120
           HI+G SITGG IDGQGT LWNCKHSGK+CP+GA  LQIND +NVNINGLSSINSQ F+IV
Sbjct: 126 HIDGLSITGGTIDGQGTALWNCKHSGKSCPVGATSLQINDAQNVNINGLSSINSQKFNIV 185

Query: 121 MNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWIE 180
           ++ CQNVHMTRVNV AA DSPNTDGIHV+QSSDVTILNS+I NGDDCISI PGTSN+W+E
Sbjct: 186 VHGCQNVHMTRVNVSAADDSPNTDGIHVQQSSDVTILNSSISNGDDCISISPGTSNLWME 245

Query: 181 SITCGPGHGI 191
           SITCGPGHGI
Sbjct: 246 SITCGPGHGI 255

[Close]

BLAST of CmaCh19G003520 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 218.4 bits (555), Expect = 5.0e-57
Identity = 101/191 (52.88%), Postives = 134/191 (70.16%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPS-KNNAITVLIDGTLVASSNIQVLANSSSWILF 60
           AC SS P TI VP   F +RSV F+G   K   +T  IDGTLVA ++ +V+ N   WI F
Sbjct: 47  ACASSRPVTIVVPKGRFLLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFF 106

Query: 61  NHINGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSI 120
            H++G ++ GG++D +G  LW+CK SGKNCP GA  +      NV ++GL+S+NSQ+F +
Sbjct: 107 QHLDGITVYGGVLDARGASLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHV 166

Query: 121 VMNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWI 180
           V+N C NV +  V VLAA +SPNTDGIHV+ SS V+I N+ I  GDDC+SIGPGT+ +WI
Sbjct: 167 VINGCNNVKLQGVKVLAAGNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWI 226

Query: 181 ESITCGPGHGI 191
           E++ CGPGHGI
Sbjct: 227 ENVACGPGHGI 237

[Close]

BLAST of CmaCh19G003520 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 216.1 bits (549), Expect = 2.5e-56
Identity = 101/191 (52.88%), Postives = 134/191 (70.16%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPS-KNNAITVLIDGTLVASSNIQVLANSSSWILF 60
           AC S +P TI VP   F +RS+ F+G   K  ++T  I GTLVA S+ +V+   + WILF
Sbjct: 49  ACASVKPVTILVPKGRFLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILF 108

Query: 61  NHINGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSI 120
            H++G S+ GG++D QG  LW+CK SGKNCP GA  +     +NV I+GL+S+NSQ+F +
Sbjct: 109 QHLDGISVYGGVLDAQGASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHV 168

Query: 121 VMNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWI 180
            +N C NV +  V V A  +SPNTDGIHV+ SS V+ILNS I  GDDC+SIGPGT+ +WI
Sbjct: 169 AINGCSNVKLDGVKVSADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWI 228

Query: 181 ESITCGPGHGI 191
           E++ CGPGHGI
Sbjct: 229 ENVACGPGHGI 239

[Close]

BLAST of CmaCh19G003520 vs. TAIR 10
Match: AT1G65570.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 191.4 bits (485), Expect = 6.5e-49
Identity = 89/192 (46.35%), Postives = 127/192 (66.15%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVR-SVRFNGPS-KNNAITVLIDGTLVASSNIQVLANSSSWIL 60
           AC   +   IYVP   + V   VRF G S K+  IT+ IDGTL+   +  +L    +W  
Sbjct: 57  ACGVEDSVVIYVPKGRYLVSGEVRFEGESCKSREITLRIDGTLIGPQDYSLLGKKENWFS 116

Query: 61  FNHINGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFS 120
           F+ ++  ++ GG  D +G+ LW+CK +G NCP GA  L+  D  NV I G+ S+NSQLF 
Sbjct: 117 FSGVHNVTVLGGSFDAKGSTLWSCKANGYNCPEGATTLRFMDSNNVKIKGVLSLNSQLFH 176

Query: 121 IVMNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMW 180
           I +NRC+N+ +  V ++A  +SPNTDGIH++ S+D+ + N++I+ GDDCISIGPGT N+ 
Sbjct: 177 IAINRCRNIKIEDVRIIAPDESPNTDGIHIQLSTDIEVRNASIKTGDDCISIGPGTKNLM 236

Query: 181 IESITCGPGHGI 191
           ++ ITCGPGHGI
Sbjct: 237 VDGITCGPGHGI 248

[Close]

BLAST of CmaCh19G003520 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 186.4 bits (472), Expect = 2.1e-47
Identity = 89/190 (46.84%), Postives = 124/190 (65.26%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNGPSKNNAITVLIDGTLVASSNIQVLANSSSWILFN 60
           AC S+   T+ VP   F ++ V F GP ++  IT  I GT+VA S+ + L NS  WILF 
Sbjct: 55  ACRSAAAVTVTVPRGSFLLKPVEFRGPCRSR-ITFQIYGTIVAPSDYRGLGNSGYWILFV 114

Query: 61  HINGFSITGGIIDGQGTLLWNCKHSGKNCPIGARGLQINDGKNVNINGLSSINSQLFSIV 120
            +N  SI GG +D +G   W C+ SGK+CP+GAR +  N   +V ++GL+SINSQ   +V
Sbjct: 115 KVNRISIIGGTLDARGASFWACRKSGKSCPVGARSMTFNWANDVVVSGLTSINSQTTHLV 174

Query: 121 MNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMWIE 180
           +N C NV + +V ++A   SPNTDG+HV+ S+ VT+ +     GDDCISIGPGT N+++ 
Sbjct: 175 INSCNNVIVRKVKLVAPDQSPNTDGLHVQGSAGVTVTDGTFHTGDDCISIGPGTRNLYMS 234

Query: 181 SITCGPGHGI 191
            + CGPGHGI
Sbjct: 235 KLNCGPGHGI 243

[Close]

BLAST of CmaCh19G003520 vs. TAIR 10
Match: AT2G43860.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 185.7 bits (470), Expect = 3.6e-47
Identity = 95/192 (49.48%), Postives = 128/192 (66.67%), Query Frame = 0


Query: 1   ACDSSEPATIYVPGSIFFVRSVRFNG-PSKNNAITVLIDGTLVASSNIQVLANSSSWILF 60
           AC S+ P TI VP   F V ++ F+G   K   I++ I G++VA  + +++A+S  WI F
Sbjct: 59  ACASANPTTIIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWF 118

Query: 61  NHINGFSITGGIIDGQGTLLWNCKHS-GKNCPIGARGLQINDGKNVNINGLSSINSQLFS 120
             +   SI GGI+D QGT LW CK++ G NCP GA+ L  +   N+ I+GL+SINSQ F 
Sbjct: 119 EDVTDVSIYGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFH 178

Query: 121 IVMNRCQNVHMTRVNVLAACDSPNTDGIHVRQSSDVTILNSNIRNGDDCISIGPGTSNMW 180
           IV++   NV++  V V A  +SPNTDGIHV  S  V I NS I  GDDCISIGPG++N++
Sbjct: 179 IVIDNSNNVNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVF 238

Query: 181 IESITCGPGHGI 191
           I++I CGPGHGI
Sbjct: 239 IQTIRCGPGHGI 250

The following BLAST results are available for this feature:

BLAST of CmaCh19G003520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P48979	1.5e-61	59.16	Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1	[more]
O22818	5.1e-46	49.48	Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386...	[more]
Q05967	1.2e-34	40.84	Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1	[more]
P49062	2.0e-34	38.02	Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ...	[more]
P26216	1.7e-33	40.41	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1	[more]

BLAST of CmaCh19G003520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IEV2	1.5e-96	93.68	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111473552 PE=3 SV=1	[more]
A0A6J1F489	6.1e-95	92.11	polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111440267 PE=3 SV=1	[more]
A0A6J1HFX3	8.8e-94	91.53	polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111463942 PE=3 SV=1	[more]
A0A6J1I843	8.3e-84	81.05	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111471802 PE=3 SV=1	[more]
A0A6J1IF00	8.3e-84	81.05	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472165 PE=3 SV=1	[more]

BLAST of CmaCh19G003520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022974795.1	3.0e-96	93.68	polygalacturonase-like [Cucurbita maxima]	[more]
XP_022933258.1	1.3e-94	92.11	polygalacturonase-like [Cucurbita moschata]	[more]
XP_022963687.1	1.8e-93	91.53	polygalacturonase-like [Cucurbita moschata]	[more]
XP_023521278.1	1.3e-86	87.89	polygalacturonase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022973584.1	1.7e-83	81.05	polygalacturonase-like [Cucurbita maxima]	[more]

BLAST of CmaCh19G003520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G43870.1	5.0e-57	52.88	Pectin lyase-like superfamily protein	[more]
AT3G59850.1	2.5e-56	52.88	Pectin lyase-like superfamily protein	[more]
AT1G65570.1	6.5e-49	46.35	Pectin lyase-like superfamily protein	[more]
AT2G43890.1	2.1e-47	46.84	Pectin lyase-like superfamily protein	[more]
AT2G43860.1	3.6e-47	49.48	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 2..190 e-value: 4.0E-43 score: 147.7
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..191 e-value: 3.1E-48 score: 166.7
None	No IPR available	PANTHER	PTHR31375:SF219	PUTATIVE-RELATED	coord: 1..190
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 1..190
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 2..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh19G003520.1	CmaCh19G003520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098656	anion transmembrane transport
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0015698	inorganic anion transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005741	mitochondrial outer membrane
molecular_function	GO:0004650	polygalacturonase activity
molecular_function	GO:0008308	voltage-gated anion channel activity