CmaCh19G003330 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh19G003330
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionpolygalacturonase-like
LocationCma_Chr19: 3407864 .. 3413164 (+)
RNA-Seq ExpressionCmaCh19G003330
SyntenyCmaCh19G003330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACTGAACAAGGAATCAGGTAGGTGAACAAAATAATTTTTTCAAATGATTTTTTTTGTATGAAAAATTAATTACCAATTATAAAATCAAATATAAAATTGGATTTTGAATGAATTAAAACACGTTTTAGAAATTTGGACAAAGGGATTTTAACTATTTTCCTTTTTACTTATGAAAATGATGAATGCAGTTACCTAATATTAATATGAATGAGAAAATGAAACCTCATGAAAAATTTTGGTATTTTGTTGAATATATATTTGTAGCATTGGGAGCTTGGGGAGGTCGGTGGAAGAGCCTGGAGTGAAAAATGTGACAGTTACATCATCAACTTTTGTCGGGACTATGAATGGAGCGAGGATTAAGTCTTGGGGAAGACCAAGCAATGGGTTCGCTACGAATATTCATTTTCAACACATTATTTTGGATAATGTGAAAAACTCTATCATAATTGATCAAAATTACTGTCCACATGACCAAGGCTGCTCCAACCAGGTATATTTTCTTATTAATCCTCGACATGTTAACTTCTTACCAACCATATTTCACTTTATATTTGATTAATAGAGCTTAGATTTTCAATTTCATACGTAATTGATTTCTTTTATCCTCAACGGATTTGAATTTATTTTGTCCATGACGTGTGTTAAATTAGGAGCATTATTTCGGCTAATACTTTTTGGGTAATGAATTTTTGTGGACAATGTAACTCTCACATATCTTCAAAAGAGTGCGATATTATCCATTTTGCTCATCATAAGCTTTTATCGTTTTATTTTATTTTATTTTTTTTTGTTTTTACGTAAAAGATCTCAAATTATTGCCCTTATTTAGTTTCATATCATATTTTGATTGTTCTTGTTTGCATGAATAGGACTCCGGAATAAAAATTAGCCAAGTAACTTACCAAGATATCAAGGGAACATCAGCCACTAAAATAGGAGTCAACTTTGATTGTAGCCCAACAAATTCTTGCACCGGACTAAGCTTGGAAGACATTAAAGTGACTTATAATTATCAAATAGCTAAAGCTTCATGCAAATATGTCAAAGGAACTGACTCTGGTGTAGTTGAACCTCCTAGTTGCTTAAAATAGACGAACGTTATTTTGGTCTTAGAGTGCATTATAGAGGTGGATAGAGGAGTTCTATTGCCTGAACTTAATAAACAAACTCTTATGATTTATTTTCTAATCGAGAAACACAACTTAGATGTAGTAATTCTTGAATCACTTCAAAAAAATTTATCAGCTTATTTTTATTAGGTAACATTATTAAAATAAATTTAACTAATAAAATTTTAATTTAATTTATGTTCTTGAATTTGTTGAACTATCATCTTAATCATTATCCTCAAATAATTTAATTTAATAAAATTATATAAACTTCTACTCATAAAAAAAAAATAATAATAATACGATTAGATGAACAAGTGTTCTCTACGGATATGATATTGTCCACTTTGAACATAGGCTTTCATGGCTTTGTTTTGGGCTTCTAAAAGATCTCACACTAATAGAAGAGAGTATTATTTTATTATAAACTCATGATCATAGAGTCGAAAGACTTTCCTCCAACAAATACTATTAAAAAAGAAAAATAATAAGAATTATAAATAGGAAAGAGTTTGAGAAATTAAGAAAAAAGGGGAAGAAATATTAAAAAAAATAAGATGAAAAGAAAAGGAAAAAGACACAAGATAAAAAAAAAAAATGTCTTGTTTAGGCATGTGGTTGGCTTTTAAGATCCCAAACTACCTTTTATGTTATAGCTTAAATAAGAAGTTTGGTACATAGAGTGTCAGTATTAATGATTTATGCACTAATTGATTCATCAAAGTTCTTTGAAATGTTTTTGAAAAATACTTGGAGATAATTACATATTTGTAGATTGTTTTGATTATTTTGAATGCATGAGAATTTCGTCTCCACGTTTTAAAATTCGTTCCTCTCGGCTCGATAAAGAAAAACCAATCGTTTCCCATCATTTCGTCCCTTGTGAAGCTGAGTCTGACCTTTTTAAGGCAAATAAGACTCTTATAAGGGTAAATTTCTCAATTCAAGTTCAATTGAGTTAAGAATTATTAAGTTACTAACGAAACGGAACAGATATTCCAATAGGAAACAATAACCGACGATTATTTGGAGCATTTTTGAGGAACGGAAGCAAACATACTCGAATTAGGGAGATCGCCGGATAAGGTCAATCAAGTATGATGACACGTGCTTGCAGCTTCGCATGTGTCATAGGCTCGTATATGCAAGTTGTCATGAAATTATATGTAAATGTTGTGTGATGTGATTTTTATGTTGTATTTGATGAATTGATATGCTTTATGATATGATTTGTTGAATATATGCTCGAGATATATTATGTGATTCTTATGATATATGATATGATATGTGCATGATAAGGATTGTTATGTCTGAAACCATGAAAACTTTATGATATTGTGACATGAATGATATGCTATGATTGACATGCAAAACGACAAACTATCATGAAATACAACTCTGAAATACGCGTGAGAGTTATGATAAATGATATGAGAAATGTTACAAGAAATGATATGAAAAATGATATGATATGAGAAATGTTATAAAAAATGATATGATATGAACAAGAAACTAGCGAAATTATATTAATGATAAATAAAATGAAAAGATGTTGGGATCTCATGCATTATTGTGTGCTCATGCATTAGGGGAAACCCCTATTTCTTCACGATAGTACAAATGCGTACGCTACGATGCAGGGGAACGATCATTATGTTTACCAAAATGCTATTGTGACGATCGCTACTCTTAATGGGTGTCTGACATCAACAGCTACCAGAGAATGCTCGTTCGACCAAAAAGGGGTCCAGAAGGTGTGCGAACCGACTAGTGGGTCCATACTCACACATGTGGACCGTGTGTAGGGAAATTAAGTACACATTCAAGATGCCCGTTAGGATAGCCGCCCTAGAAAGATAGATTGTTATGATGGGTCTCATGCATGAAACTATATATGTTTGCATCTAACTCCGATAACAGGGTCGCTTACCGAGTATTTATTAAAATACACAAACCACGTGCTACTCTACTCTTCAAGTAAAAGCAAGGCTCGCTTGTACAGCTGACGGTGACATTGCAACTATGAGACTATGGCACATGGTTAGGATAATTTCGTAATTTGAATTTATTAGACTATAGGTTAGTATAGATAAGTGCATTTCCTTTAGTAGCATATAGGTTAGTATAGGGCATTTTACTCTATGTTATTTTTCTTATTAATTCAATTTGTATTGTTTTATTTCAAAAGCATTAGAAGTGACATGGAAGGACAAGCTCAGGTTTTATTATAAAGTTTCGTATTATAAAGTTTCACCTGAGGTATTTCTGCATTAAATGTTCATGATATGTATATGGTAACGACTCTTAAGTTAAGTAAAAATTTATGGTCGTTATAGTAATGGTGAGAGATTATTTTGAGTATCCGTGTAACGCACCCAAAATTTCAATCTAAATTAGGGAATATCTCTACAATTTATTACTTGCTTTGTATTGTCTCATGCCCAAAGTTTGAATATAGATTCAGCACGTGCTCCGACATTTTTCTACGACATGGTGCGTTGTTTACTTCTCGCTTTTAAGAATGAAGACTATCCCCACAAACTAACACGAGTGCTTTACACATGTCTTGTCTTCACTTACATGTTTCATGAGAACATTCTCAAGAGGTCACCTAATATAGCATTGCTATAAGTTACGTGCATTTAACCGTGGAGTTTAACTGTGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTGTNAAGAGGTCACCTAATATAGCATTGCTATAAGTTACGTGCATTTAACCGTGGAGTTTAACTGTGTTTTTTTTTTTTTTTTTTCTTTGTTTTGTTTGGTTTGTGGTAAGAATCAATATGGGCATGACAGTGCAAATAGTCAATTCATATTCATAATCATACATAACTAATATCATCAATAACCACATATAAGGTTGCCTAGACACTATTGTCTATTAACTTTCACTCTTTCACAAGTCATCAATATTACTCCAGAAGATATAGATACCGATAGCTCATAGCCTAACTACTCTATTTTAAGTTGAGCTTGATGAACAAATTTAAACAAAATAAAAGAATGTGTAGAGCCTGAATCAAATATAGTGTAAGCATAATAGTCGCACAAAGGTAAAGTACTTGTTACCACGATACTCACATCCTCCACTACTTTGCTAGTCAAGGCATAGGCTTTACCTCAAGCCTGATCAAGCCTGATTCTGACAACCAGCACTTGTTTGACTACCTCCATCTCATGAGAAAATTCTCTCAATTTTTACTTCATTAGGATATATCGCATTTCGACACATTCTTGGAGATCGATGATCAATATATCATTATACTACCTATTGTTGGGATCCATAAGACATCTAAGTATACTCCGCTTACTCCCCATTCGCATCTGACTGCTAGAACTATAGTTACTTCTTGGAGTATTTATATCCAATTTAATAGCAACACAAAACCTGTATCTCCCACTTCAAACCTTTGATAAAGAGCTTTGTGTTCTCTTCTTCAGTACCAACCAAATAAGGAGAAAATCTAGATAATTGTGTAAACTATAAATCGTACTCGTCCATGGATCGATTACCTTGCTTCAGCTCCAAAAATATTATCTGATTACAAAATCGCACCAAAGTAGGGTAATATTTTTCACAAAGCTCTTGTTTAAATGCCAACCAAGAAATCGGACCTCCACTAGTATCCATATTAGACTCTTTTGATTTTCACAAAAAAATAAATTGGTCCTGCTAGCATGTACGTGGCACATTGAACCTTGTAGCTTTTCAGGTTGTTCGCGTATCTAAACTTCGTTTCAATAGACTCCAACCACCATTCAGCTGCCTCAAGATCTACCACTCTACCTTCAAAAGTATGTGGTTCATATCGCATAAAATCATGTAAGTACCCACTCTCCTCCATCATAAAACGTTTATCATATGGTATTATTTTACTAACCGCATTTTGCAATTTTCTTTATTTCCATTCGGAGGGTAAGAAGTTTCATAAACTCGTTTTTTTATCTTTTGATACTTGTTTTTCCTTAGGTGGTGTCTCTATCAAGACTACTGTACTTGGTGGCACTATCGGATTTGGTGGAGGTATTGATGTCTGAACATCTATTCCAGGTACTTCAACAAATGGACTTGCTTGATTAACCACTAGGGGTATGGGTGCAGTTGGAGTTGTCTGACTCCAAAATCCAGCAAGGGGCCGTCTGCAATGCGTGA

mRNA sequence

ATGGAACTGAACAAGGAATCAGGTAGCATTGGGAGCTTGGGGAGGTCGGTGGAAGAGCCTGGAGTGAAAAATGTGACAGTTACATCATCAACTTTTGTCGGGACTATGAATGGAGCGAGGATTAAGTCTTGGGGAAGACCAAGCAATGGGTTCGCTACGAATATTCATTTTCAACACATTATTTTGGATAATGTGAAAAACTCTATCATAATTGATCAAAATTACTGTCCACATGACCAAGGCTGCTCCAACCAGGACTCCGGAATAAAAATTAGCCAAGTAACTTACCAAGATATCAAGGGAACATCAGCCACTAAAATAGGAGTCAACTTTGATTGTAGCCCAACAAATTCTTGCACCGGACTAAGCTTGGAAGACATTAAAGTGGTGTCTCTATCAAGACTACTGTACTTGGTGGCACTATCGGATTTGGTGGAGGTATTGATGTCTGAACATCTATTCCAGTTGGAGTTGTCTGACTCCAAAATCCAGCAAGGGGCCGTCTGCAATGCGTGA

Coding sequence (CDS)

ATGGAACTGAACAAGGAATCAGGTAGCATTGGGAGCTTGGGGAGGTCGGTGGAAGAGCCTGGAGTGAAAAATGTGACAGTTACATCATCAACTTTTGTCGGGACTATGAATGGAGCGAGGATTAAGTCTTGGGGAAGACCAAGCAATGGGTTCGCTACGAATATTCATTTTCAACACATTATTTTGGATAATGTGAAAAACTCTATCATAATTGATCAAAATTACTGTCCACATGACCAAGGCTGCTCCAACCAGGACTCCGGAATAAAAATTAGCCAAGTAACTTACCAAGATATCAAGGGAACATCAGCCACTAAAATAGGAGTCAACTTTGATTGTAGCCCAACAAATTCTTGCACCGGACTAAGCTTGGAAGACATTAAAGTGGTGTCTCTATCAAGACTACTGTACTTGGTGGCACTATCGGATTTGGTGGAGGTATTGATGTCTGAACATCTATTCCAGTTGGAGTTGTCTGACTCCAAAATCCAGCAAGGGGCCGTCTGCAATGCGTGA

Protein sequence

MELNKESGSIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNSIIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIKVVSLSRLLYLVALSDLVEVLMSEHLFQLELSDSKIQQGAVCNA
Homology
BLAST of CmaCh19G003330 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 4.5e-38
Identity = 75/121 (61.98%), Postives = 93/121 (76.86%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLG+  EE GV+NVTV + TF GT NG RIKSWGRPS GFA NI FQH  + NV+N 
Sbjct: 245 SIGSLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVNVENP 304

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           I+IDQ+YCP ++GC  Q SG++IS VTY+DI GTSAT++ V FDCSP + C  + LED+K
Sbjct: 305 IVIDQHYCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVK 364

Query: 129 V 130
           +
Sbjct: 365 L 365

BLAST of CmaCh19G003330 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.6e-33
Identity = 71/126 (56.35%), Postives = 92/126 (73.02%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLGR+ EE GV NVTV++  F+GT NG RIK+WG+ SN FA NI FQHI +  VKN 
Sbjct: 251 SIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNP 310

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQ+YC H + C  Q+SG+K+S V Y+DI GTS T++ V  DCS    CTG+ ++D+ 
Sbjct: 311 IIIDQHYCLH-KPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVN 370

Query: 129 VVSLSR 135
           +VS+ R
Sbjct: 371 LVSVHR 375

BLAST of CmaCh19G003330 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.3e-24
Identity = 56/119 (47.06%), Postives = 76/119 (63.87%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLG    E  V +V V  +   GT NG RIK+W +  +G A+NI FQ++ + NV+N 
Sbjct: 309 SIGSLGYGNSEAHVSDVVVNGAKLCGTTNGVRIKTW-QGGSGSASNIKFQNVEMHNVENP 368

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDI 128
           IIIDQNYC  D+ C  Q S +++  V YQ+IKGT A+ + + FDCS    C G+ LED+
Sbjct: 369 IIIDQNYCDQDKPCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDV 426

BLAST of CmaCh19G003330 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 8.3e-24
Identity = 57/122 (46.72%), Postives = 79/122 (64.75%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLG    E  V NV V  +T +GT NG RIK+W +  +G A NI FQ II+ NV N 
Sbjct: 291 SIGSLGEDNSEAYVSNVVVNKATLIGTTNGVRIKTW-QGGHGMAKNIIFQDIIMKNVTNP 350

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           III+Q+YC   + C  Q S +++S V Y++I+GTS+  I V F CS    C G+S++++K
Sbjct: 351 IIINQDYCDRVEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVK 410

Query: 129 VV 131
           +V
Sbjct: 411 LV 411

BLAST of CmaCh19G003330 vs. ExPASy Swiss-Prot
Match: P05117 (Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 1.8e-23
Identity = 57/125 (45.60%), Postives = 79/125 (63.20%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLG    E  V NVTV  +  +G  NG RIK+W +  +G A+NI F ++ + +VK  
Sbjct: 296 SIGSLGSGNSEAYVSNVTVNEAKIIGAENGVRIKTW-QGGSGQASNIKFLNVEMQDVKYP 355

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYC   + C  Q S +++  V Y++IKGTSATK+ + FDCS    C G+ +E+I 
Sbjct: 356 IIIDQNYCDRVEPCIQQFSAVQVKNVVYENIKGTSATKVAIKFDCSTNFPCEGIIMENIN 415

Query: 129 VVSLS 134
           +V  S
Sbjct: 416 LVGES 419

BLAST of CmaCh19G003330 vs. ExPASy TrEMBL
Match: A0A6J1F3I5 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439547 PE=3 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.3e-51
Identity = 100/121 (82.64%), Postives = 111/121 (91.74%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLGRS +EPGVKNVTVTSSTF G+ NG RIKSW RPSNGFAT+IHFQHI LDNVKN 
Sbjct: 245 SIGSLGRSSDEPGVKNVTVTSSTFTGSTNGVRIKSWARPSNGFATDIHFQHITLDNVKNP 304

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYCPHD+GC +QDSG+KIS+VTY+DIKGTSAT+IGV FDC+PTN CTGL+LEDIK
Sbjct: 305 IIIDQNYCPHDKGCPHQDSGVKISEVTYEDIKGTSATEIGVKFDCNPTNPCTGLTLEDIK 364

Query: 129 V 130
           V
Sbjct: 365 V 365

BLAST of CmaCh19G003330 vs. ExPASy TrEMBL
Match: A0A6J1IDK7 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472169 PE=3 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.8e-51
Identity = 101/121 (83.47%), Postives = 109/121 (90.08%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLG+S  EPGVKNVTVTSSTF  T NG RIKSWGRPS+GFAT+IHFQHI LDNVKN 
Sbjct: 245 SIGSLGKSANEPGVKNVTVTSSTFSETTNGVRIKSWGRPSSGFATDIHFQHITLDNVKNP 304

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYCPHD+GC +QDSG+KIS VTY+DIKGTSATKIGV FDCSPTN CTGL+LEDIK
Sbjct: 305 IIIDQNYCPHDKGCPHQDSGVKISDVTYEDIKGTSATKIGVKFDCSPTNPCTGLTLEDIK 364

Query: 129 V 130
           V
Sbjct: 365 V 365

BLAST of CmaCh19G003330 vs. ExPASy TrEMBL
Match: A0A6J1I926 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472172 PE=3 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.8e-51
Identity = 101/121 (83.47%), Postives = 109/121 (90.08%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLG+S  EPGVKNVTVTSSTF  T NG RIKSWGRPS+GFAT+IHFQHI LDNVKN 
Sbjct: 245 SIGSLGKSANEPGVKNVTVTSSTFSETTNGVRIKSWGRPSSGFATDIHFQHITLDNVKNP 304

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYCPHD+GC +QDSG+KIS VTY+DIKGTSATKIGV FDCSPTN CTGL+LEDIK
Sbjct: 305 IIIDQNYCPHDKGCPHQDSGVKISDVTYEDIKGTSATKIGVKFDCSPTNPCTGLTLEDIK 364

Query: 129 V 130
           V
Sbjct: 365 V 365

BLAST of CmaCh19G003330 vs. ExPASy TrEMBL
Match: A0A6J1IEE1 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472451 PE=3 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.8e-50
Identity = 98/121 (80.99%), Postives = 110/121 (90.91%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLG+S +EPGVKNVTVTSSTF  T NG RIKSWGRPS+GFAT+IHFQHI L+NVKN 
Sbjct: 245 SIGSLGKSADEPGVKNVTVTSSTFTETTNGVRIKSWGRPSSGFATDIHFQHITLENVKNP 304

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYCPHD+GC +QDSG+KIS+VTY+DIKGTSAT+IGV FDCSPTN C GL+LEDIK
Sbjct: 305 IIIDQNYCPHDEGCPHQDSGVKISEVTYEDIKGTSATEIGVKFDCSPTNPCIGLTLEDIK 364

Query: 129 V 130
           V
Sbjct: 365 V 365

BLAST of CmaCh19G003330 vs. ExPASy TrEMBL
Match: A0A6J1F362 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439543 PE=3 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.8e-50
Identity = 98/121 (80.99%), Postives = 110/121 (90.91%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLG+S +EPGVKNVTVTSSTF  + NG RIKSW RPS+GFAT+IHFQHI LDNVKN 
Sbjct: 245 SIGSLGKSADEPGVKNVTVTSSTFTESTNGVRIKSWARPSSGFATDIHFQHITLDNVKNP 304

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYCPHD+GC +QDSG+KIS+VTY+DIKGTSAT+IGV FDCSPTN CTGL+LEDIK
Sbjct: 305 IIIDQNYCPHDEGCPHQDSGVKISEVTYEDIKGTSATEIGVKFDCSPTNPCTGLTLEDIK 364

Query: 129 V 130
           V
Sbjct: 365 V 365

BLAST of CmaCh19G003330 vs. NCBI nr
Match: XP_023511394.1 (polygalacturonase-like [Cucurbita pepo subsp. pepo] >XP_023511396.1 polygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 223.4 bits (568), Expect = 1.5e-54
Identity = 105/121 (86.78%), Postives = 114/121 (94.21%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           S+GSLG+S +EPGVKNVTVTSSTFVGT NG RIKSWGRPSNGFATNIHFQHIILDNV+N 
Sbjct: 245 SVGSLGKSADEPGVKNVTVTSSTFVGTTNGVRIKSWGRPSNGFATNIHFQHIILDNVENP 304

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYCPHDQGC  QDSG+KIS+VTY+DIKGTSAT+IGVNFDCSPTN CTGLSLE+IK
Sbjct: 305 IIIDQNYCPHDQGCPGQDSGVKISEVTYEDIKGTSATEIGVNFDCSPTNPCTGLSLENIK 364

Query: 129 V 130
           V
Sbjct: 365 V 365

BLAST of CmaCh19G003330 vs. NCBI nr
Match: XP_023511563.1 (polygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 222.2 bits (565), Expect = 3.3e-54
Identity = 104/121 (85.95%), Postives = 114/121 (94.21%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           ++GSLG+S +EPGVKNVTVTSSTFVGT NG RIKSWGRPSNGFATNIHFQHIILDNV+N 
Sbjct: 57  NVGSLGKSADEPGVKNVTVTSSTFVGTTNGVRIKSWGRPSNGFATNIHFQHIILDNVENP 116

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYCPHDQGC  QDSG+KIS+VTY+DIKGTSAT+IGVNFDCSPTN CTGLSLE+IK
Sbjct: 117 IIIDQNYCPHDQGCPGQDSGVKISEVTYEDIKGTSATEIGVNFDCSPTNPCTGLSLENIK 176

Query: 129 V 130
           V
Sbjct: 177 V 177

BLAST of CmaCh19G003330 vs. NCBI nr
Match: XP_022932930.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 212.6 bits (540), Expect = 2.6e-51
Identity = 100/121 (82.64%), Postives = 111/121 (91.74%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLGRS +EPGVKNVTVTSSTF G+ NG RIKSW RPSNGFAT+IHFQHI LDNVKN 
Sbjct: 245 SIGSLGRSSDEPGVKNVTVTSSTFTGSTNGVRIKSWARPSNGFATDIHFQHITLDNVKNP 304

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYCPHD+GC +QDSG+KIS+VTY+DIKGTSAT+IGV FDC+PTN CTGL+LEDIK
Sbjct: 305 IIIDQNYCPHDKGCPHQDSGVKISEVTYEDIKGTSATEIGVKFDCNPTNPCTGLTLEDIK 364

Query: 129 V 130
           V
Sbjct: 365 V 365

BLAST of CmaCh19G003330 vs. NCBI nr
Match: XP_022973588.1 (polygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 211.5 bits (537), Expect = 5.9e-51
Identity = 101/121 (83.47%), Postives = 109/121 (90.08%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLG+S  EPGVKNVTVTSSTF  T NG RIKSWGRPS+GFAT+IHFQHI LDNVKN 
Sbjct: 245 SIGSLGKSANEPGVKNVTVTSSTFSETTNGVRIKSWGRPSSGFATDIHFQHITLDNVKNP 304

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYCPHD+GC +QDSG+KIS VTY+DIKGTSATKIGV FDCSPTN CTGL+LEDIK
Sbjct: 305 IIIDQNYCPHDKGCPHQDSGVKISDVTYEDIKGTSATKIGVKFDCSPTNPCTGLTLEDIK 364

Query: 129 V 130
           V
Sbjct: 365 V 365

BLAST of CmaCh19G003330 vs. NCBI nr
Match: XP_022973591.1 (polygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 211.5 bits (537), Expect = 5.9e-51
Identity = 101/121 (83.47%), Postives = 109/121 (90.08%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLG+S  EPGVKNVTVTSSTF  T NG RIKSWGRPS+GFAT+IHFQHI LDNVKN 
Sbjct: 245 SIGSLGKSANEPGVKNVTVTSSTFSETTNGVRIKSWGRPSSGFATDIHFQHITLDNVKNP 304

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYCPHD+GC +QDSG+KIS VTY+DIKGTSATKIGV FDCSPTN CTGL+LEDIK
Sbjct: 305 IIIDQNYCPHDKGCPHQDSGVKISDVTYEDIKGTSATKIGVKFDCSPTNPCTGLTLEDIK 364

Query: 129 V 130
           V
Sbjct: 365 V 365

BLAST of CmaCh19G003330 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 159.1 bits (401), Expect = 3.2e-39
Identity = 73/121 (60.33%), Postives = 93/121 (76.86%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLG+   E GV+N+TV ++TF GT NG RIKSW RPSNGFA NI FQH +++NV+N 
Sbjct: 240 SIGSLGKESVEVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQNP 299

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           I+IDQNYCP ++ C NQ SGIKIS V + DI GTSAT++GV  DCS    CTG+ ++D+K
Sbjct: 300 IVIDQNYCPGNENCPNQVSGIKISDVMFFDIHGTSATEVGVKLDCSSKKPCTGIRIQDVK 359

Query: 129 V 130
           +
Sbjct: 360 L 360

BLAST of CmaCh19G003330 vs. TAIR 10
Match: AT1G05650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 154.8 bits (390), Expect = 6.1e-38
Identity = 71/121 (58.68%), Postives = 95/121 (78.51%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSL + + E GV+NVTV+SS F G+ NG RIKSW RPS GF  N+ FQ++I+ NV+N 
Sbjct: 243 SIGSLAKQLNEDGVENVTVSSSVFTGSQNGVRIKSWARPSTGFVRNVFFQNLIMRNVQNP 302

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYCP +QGC  + SG+KI+QVTY++I+GTSAT+  +   CS +N CTG++L+DIK
Sbjct: 303 IIIDQNYCPSNQGCPTEHSGVKITQVTYKNIQGTSATQEAMKLVCSKSNPCTGITLQDIK 362

Query: 129 V 130
           +
Sbjct: 363 L 363

BLAST of CmaCh19G003330 vs. TAIR 10
Match: AT2G43880.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 154.5 bits (389), Expect = 7.9e-38
Identity = 73/126 (57.94%), Postives = 93/126 (73.81%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLG    E GV+NVTVTSS F  T NG RIK+W RPS GF  N+ F+++I++NV+N 
Sbjct: 246 SIGSLGDYANEEGVQNVTVTSSVFTKTQNGVRIKTWARPSRGFVNNVVFRNLIMNNVENP 305

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           +IIDQNYCP+ +GC  Q SG+KIS VT+ +IKGTS T I +  DCS +N CTGL L+DIK
Sbjct: 306 VIIDQNYCPNGKGCPRQSSGVKISGVTFANIKGTSTTPIAMKLDCSGSNHCTGLRLQDIK 365

Query: 129 VVSLSR 135
           +  + R
Sbjct: 366 LTYMRR 371

BLAST of CmaCh19G003330 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 153.7 bits (387), Expect = 1.4e-37
Identity = 76/121 (62.81%), Postives = 90/121 (74.38%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSLG+   E GV+NVTV + TF GT NG RIKSW RPS+GFA NI FQH +++NV+N 
Sbjct: 238 SIGSLGKDSVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENP 297

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYCP D  C  Q SGIKIS V + DI GTSAT++GV  DCS    CTG+ LED+K
Sbjct: 298 IIIDQNYCP-DHDCPRQVSGIKISDVLFVDIHGTSATEVGVKLDCSSKKPCTGIRLEDVK 357

Query: 129 V 130
           +
Sbjct: 358 L 357

BLAST of CmaCh19G003330 vs. TAIR 10
Match: AT1G05660.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 152.9 bits (385), Expect = 2.3e-37
Identity = 70/121 (57.85%), Postives = 95/121 (78.51%), Query Frame = 0

Query: 9   SIGSLGRSVEEPGVKNVTVTSSTFVGTMNGARIKSWGRPSNGFATNIHFQHIILDNVKNS 68
           SIGSL + ++E GV+NVTV+SS F G+ NG RIKSW RPSNGF   + FQ +++ NV+N 
Sbjct: 243 SIGSLAKELKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVFFQDLVMKNVENP 302

Query: 69  IIIDQNYCPHDQGCSNQDSGIKISQVTYQDIKGTSATKIGVNFDCSPTNSCTGLSLEDIK 128
           IIIDQNYCP  +GC N+ SG+KISQVTY++I+GTSAT+  +   CS ++ CTG++L+DIK
Sbjct: 303 IIIDQNYCPTHEGCPNEYSGVKISQVTYKNIQGTSATQEAMKLVCSKSSPCTGITLQDIK 362

Query: 129 V 130
           +
Sbjct: 363 L 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P489794.5e-3861.98Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1[more]
O228182.6e-3356.35Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386... [more]
P353361.3e-2447.06Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Q9SFB78.3e-2446.72Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2[more]
P051171.8e-2345.60Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F3I51.3e-5182.64polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439547 PE=3 SV=1[more]
A0A6J1IDK72.8e-5183.47polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472169 PE=3 SV=1[more]
A0A6J1I9262.8e-5183.47polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472172 PE=3 SV=1[more]
A0A6J1IEE11.8e-5080.99polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472451 PE=3 SV=1[more]
A0A6J1F3621.8e-5080.99polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439543 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023511394.11.5e-5486.78polygalacturonase-like [Cucurbita pepo subsp. pepo] >XP_023511396.1 polygalactur... [more]
XP_023511563.13.3e-5485.95polygalacturonase-like [Cucurbita pepo subsp. pepo][more]
XP_022932930.12.6e-5182.64polygalacturonase-like [Cucurbita moschata][more]
XP_022973588.15.9e-5183.47polygalacturonase-like [Cucurbita maxima][more]
XP_022973591.15.9e-5183.47polygalacturonase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G59850.13.2e-3960.33Pectin lyase-like superfamily protein [more]
AT1G05650.16.1e-3858.68Pectin lyase-like superfamily protein [more]
AT2G43880.17.9e-3857.94Pectin lyase-like superfamily protein [more]
AT2G43870.11.4e-3762.81Pectin lyase-like superfamily protein [more]
AT1G05660.12.3e-3757.85Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 4..141
e-value: 3.3E-36
score: 127.1
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 9..131
e-value: 3.3E-32
score: 111.9
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 9..130
NoneNo IPR availablePANTHERPTHR31375:SF219PUTATIVE-RELATEDcoord: 9..130
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 9..133

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh19G003330.1CmaCh19G003330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity