CmaCh19G001660 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh19G001660
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Poly(A)-specific ribonuclease
Location	Cma_Chr19: 1110779 .. 1111755 (-)
RNA-Seq Expression	CmaCh19G001660
Synteny	CmaCh19G001660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAAAATTATTGGAGAAGCTGCTATAGTTGGAGGCAAATGGAAGAGAGATGTCTGGTCTCACAATTTATTGGAGGAGATAGAACTCATGAAGCACTGTTTATCAGAATTTCCAATTGTGGCTATGGATACGGAGTTTCCAGGTAAAAATCAAACTTGAAGGATGGTGAGAAGTAGTGATTTTTTAGTCTTTGTTTTACTGACTCTTGAAATCTTTTTAGTCTTTGTTGTACTGACGTTTGAATGTATCTCCTCTTTTTAGGATTTTTAAGATGTACTCCCAAAGGTGTTTCAGATGAAGAATTCTACCAATGCCTCCAATTTAACGTAAATCGACTGAGCATACTTCAGTTAGGACTCACACTTACCGATGAGAATGGAAGAATAGGAATGACGTGGGAGTTTAACTTTCAAGATTTTGATAAAATTAGTAATATGGAGGGTGTTTATTCACTCGATTCATTTCTAAAGAATAAGGGATTTAATTTCAATATCCTGAGGAGCTGTGGAATTTCTCCGGAAGAATTTAGTAGACAATTTAACCCTATCCTTCGAAGCGGTAGAATACAAAGATGGATAACATTTCATGGATTGTATGACATCGCCTATTTGATGAAGTTTACCAACATAATATGCATGCCTACGTCCATGTCTAACTTCGCCATCATGGCTGCACATCTATTAGGAACAGTCACCGACCTGAAACATATGGCTCGGTACTCCGACTACCTACTCAATGGCAACCTAGGCTTAAAAAAACTGGCGAAGATGCTGCAAGTTAATCGCATCGGTACTGCCCACTTTGCTGGGTCTGACAGTTTCCTTACAGCATCCGTTTATGCCAAGATGAGGGTAATTATGAAGGTTGATCCAAAGATACACGAAGGCTTTTTATATGGATTACCATATCCAATTCAGGGTTATCGACCACCAGTTGTAATTGTAGATCCAAAGTTAACCCACAATCGTATCTAA

mRNA sequence

ATGGCAAAAATTATTGGAGAAGCTGCTATAGTTGGAGGCAAATGGAAGAGAGATGTCTGGTCTCACAATTTATTGGAGGAGATAGAACTCATGAAGCACTGTTTATCAGAATTTCCAATTGTGGCTATGGATACGGAGTTTCCAGGATTTTTAAGATGTACTCCCAAAGGTGTTTCAGATGAAGAATTCTACCAATGCCTCCAATTTAACGTAAATCGACTGAGCATACTTCAGTTAGGACTCACACTTACCGATGAGAATGGAAGAATAGGAATGACGTGGGAGTTTAACTTTCAAGATTTTGATAAAATTAGTAATATGGAGGGTGTTTATTCACTCGATTCATTTCTAAAGAATAAGGGATTTAATTTCAATATCCTGAGGAGCTGTGGAATTTCTCCGGAAGAATTTAGTAGACAATTTAACCCTATCCTTCGAAGCGGTAGAATACAAAGATGGATAACATTTCATGGATTGTATGACATCGCCTATTTGATGAAGTTTACCAACATAATATGCATGCCTACGTCCATGTCTAACTTCGCCATCATGGCTGCACATCTATTAGGAACAGTCACCGACCTGAAACATATGGCTCGGTACTCCGACTACCTACTCAATGGCAACCTAGGCTTAAAAAAACTGGCGAAGATGCTGCAAGTTAATCGCATCGGTACTGCCCACTTTGCTGGGTCTGACAGTTTCCTTACAGCATCCGTTTATGCCAAGATGAGGGTAATTATGAAGGTTGATCCAAAGATACACGAAGGCTTTTTATATGGATTACCATATCCAATTCAGGGTTATCGACCACCAGTTGTAATTGTAGATCCAAAGTTAACCCACAATCGTATCTAA

Coding sequence (CDS)

ATGGCAAAAATTATTGGAGAAGCTGCTATAGTTGGAGGCAAATGGAAGAGAGATGTCTGGTCTCACAATTTATTGGAGGAGATAGAACTCATGAAGCACTGTTTATCAGAATTTCCAATTGTGGCTATGGATACGGAGTTTCCAGGATTTTTAAGATGTACTCCCAAAGGTGTTTCAGATGAAGAATTCTACCAATGCCTCCAATTTAACGTAAATCGACTGAGCATACTTCAGTTAGGACTCACACTTACCGATGAGAATGGAAGAATAGGAATGACGTGGGAGTTTAACTTTCAAGATTTTGATAAAATTAGTAATATGGAGGGTGTTTATTCACTCGATTCATTTCTAAAGAATAAGGGATTTAATTTCAATATCCTGAGGAGCTGTGGAATTTCTCCGGAAGAATTTAGTAGACAATTTAACCCTATCCTTCGAAGCGGTAGAATACAAAGATGGATAACATTTCATGGATTGTATGACATCGCCTATTTGATGAAGTTTACCAACATAATATGCATGCCTACGTCCATGTCTAACTTCGCCATCATGGCTGCACATCTATTAGGAACAGTCACCGACCTGAAACATATGGCTCGGTACTCCGACTACCTACTCAATGGCAACCTAGGCTTAAAAAAACTGGCGAAGATGCTGCAAGTTAATCGCATCGGTACTGCCCACTTTGCTGGGTCTGACAGTTTCCTTACAGCATCCGTTTATGCCAAGATGAGGGTAATTATGAAGGTTGATCCAAAGATACACGAAGGCTTTTTATATGGATTACCATATCCAATTCAGGGTTATCGACCACCAGTTGTAATTGTAGATCCAAAGTTAACCCACAATCGTATCTAA

Protein sequence

MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSDEEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNKGFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSNFAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASVYAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI

Homology

[Close]

BLAST of CmaCh19G001660 vs. ExPASy Swiss-Prot
Match: Q9LXM4 (Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=CAF1-8 PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.4e-39
Identity = 95/220 (43.18%), Postives = 132/220 (60.00%), Query Frame = 0


Query: 28  IELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSDEEFYQCLQFNVNRLSILQLGLTLTDEN 87
           + L++ CL  +  +A+DTEFP  LR T +  +DEE Y  + F+V+R  ++QLGLTL D N
Sbjct: 1   MSLIEDCLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDIN 60

Query: 88  GRIGMTWEFNFQDF--DKISNMEGVYSLDSFLKNKGFNFNILRSCGISPEE-FSRQFNPI 147
           GRIG TWE NF DF  D   N + +     FL+  G +   +R  GI  E  FS  F  +
Sbjct: 61  GRIGGTWEINFSDFGVDDARNEKSI----EFLRRNGLDLRKIREEGIRIEGFFSEMFWML 120

Query: 148 LRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSNFAIMAAHLLGTVTDLKHMARYSDY 207
            ++ R   W+TFHG YDIAYL+K      +P +   F+   A +LG+V DLK MA   + 
Sbjct: 121 KKTRRNITWVTFHGSYDIAYLLKGFTGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEG 180

Query: 208 LLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASVYAKM 245
            L+  LGL+ LA    +NR+GTAH AGS++ LTA V+AK+
Sbjct: 181 -LSSRLGLETLAHEFGLNRVGTAHHAGSNNELTAMVFAKV 215

[Close]

BLAST of CmaCh19G001660 vs. ExPASy Swiss-Prot
Match: Q9SHJ0 (Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=CAF1-1 PE=2 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.5e-38
Identity = 90/250 (36.00%), Postives = 136/250 (54.40%), Query Frame = 0


Query: 17  RDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSDEEFYQCLQFNVNRLSI 76
           R VW  N+ EE+  M  CL  FP++A DTE+PG +  T    S +E Y+ ++ NV    +
Sbjct: 10  RRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVENTKL 69

Query: 77  LQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNKGFNFNILRSCGISPEE 136
           +Q G TL +  G IG  WE NF +F   S+     S++ FL+  G +   +R  G+  + 
Sbjct: 70  IQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIE-FLRRHGLDLQKIRDEGV--DM 129

Query: 137 FSRQFNP----ILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSNFAIMAAHLLGTV 196
           F   F P    + RS +   ++TF G YD AY +   N   +P +   FA     + G V
Sbjct: 130 FGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILNHGKLPETHGEFATEVVKVFGQV 189

Query: 197 TDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASVYAKMRVIMKVDP 256
            D K MA + +  L  +LGL KLA++LQ+ R+G AH AGSDS +TA V+ K++ + + D 
Sbjct: 190 YDTKVMAGFCEG-LGEHLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE-DS 249

Query: 257 KIHEGFLYGL 263
           +   G +YG+
Sbjct: 250 RFARGLIYGI 254

[Close]

BLAST of CmaCh19G001660 vs. ExPASy Swiss-Prot
Match: Q9LXM2 (Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=CAF1-9 PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 9.9e-38
Identity = 97/266 (36.47%), Postives = 149/266 (56.02%), Query Frame = 0


Query: 17  RDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFL-RCTPKGVSDEEFYQCLQFNVNRLS 76
           R+VW+ NL  E EL+   + ++P ++MDTEFPG + +   +  + ++ Y  L+ NV+ LS
Sbjct: 20  REVWAENLESEFELISEIIDDYPFISMDTEFPGVIFKSDLRFTNPDDLYTLLKANVDALS 79

Query: 77  ILQLGLTLTDENGRI---------GMTWEFNFQDFDKISNMEGVYSLDSFLKNKGFNFNI 136
           ++Q+GLTL+D NG +         G  WEFNF+DFD   +     S++  L+ +G +F  
Sbjct: 80  LIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNFRDFDVARDAHAPDSIE-LLRRQGIDFE- 139

Query: 137 LRSC--GISPEEFSRQFNPILRSGRI----QRWITFHGLYDIAYLMKFTNIICMPTSMSN 196
            R+C  G+  E F+     ++ SG +      W+TFH  YD  YLMK      +P ++  
Sbjct: 140 -RNCRDGVESERFAEL---MMSSGLVCNEEVSWVTFHSAYDFGYLMKILTRRELPGALGE 199

Query: 197 FAIMAAHLLG-TVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNR-IGTAHFAGSDSFLTA 256
           F  +   L G  V D+KHM ++ +  L G  GL ++A+ L+VNR +G  H AGSDS LT 
Sbjct: 200 FKRVMRVLFGERVYDVKHMMKFCERRLFG--GLDRVARTLEVNRAVGKCHQAGSDSLLTW 259

Query: 257 SVYAKMRVIMKVD--PKIHEGFLYGL 263
             + +MR +  V   P+ H G LYGL
Sbjct: 260 HAFQRMRDLYFVQDGPEKHAGVLYGL 277

[Close]

BLAST of CmaCh19G001660 vs. ExPASy Swiss-Prot
Match: O64773 (Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=CAF1-5 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 4.9e-37
Identity = 93/230 (40.43%), Postives = 136/230 (59.13%), Query Frame = 0


Query: 18  DVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSDEEFYQCLQFNVNRLSIL 77
           +VW  N   E+  ++ CL     +A+DTEFPG L+ TP   SDE  Y+ ++FNV+   ++
Sbjct: 4   EVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLI 63

Query: 78  QLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNKGFNFNILRSCGISPEEF 137
           QLGLTL  +   I  TWE N  DF++  +++   S+ +FLKN G + + +R  GI  EEF
Sbjct: 64  QLGLTLFGKG--ITKTWEINLSDFNESKSLKNDKSI-AFLKNNGLDLDKIREEGIGIEEF 123

Query: 138 SRQFNPIL--RSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSNFAIMAAHLLGT-VTD 197
             +F+ IL  + G++ RW+TF G YD AYL+K      +P +   F      LLG  V D
Sbjct: 124 FMEFSQILNEKHGKM-RWVTFQGSYDKAYLLKGLTRKPLPETSKEFDETVQQLLGRFVYD 183

Query: 198 LKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASVYAKM 245
           +K MA      L+   GL+++A +LQ+ R+G AH AGSDS LTA V+ K+
Sbjct: 184 VKKMAGLCSG-LSSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKL 228

[Close]

BLAST of CmaCh19G001660 vs. ExPASy Swiss-Prot
Match: Q9S9P2 (Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=CAF1-2 PE=1 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.1e-36
Identity = 91/267 (34.08%), Postives = 145/267 (54.31%), Query Frame = 0


Query: 17  RDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCT--------PKGVSDEEFYQCLQ 76
           R+VW+HNL +E+ L++  + +FP VAMDTEFPG +  T        P  +  E  Y  L+
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYNYDTLK 76

Query: 77  FNVNRLSILQLGLTLTDENGRI-------GMTWEFNFQDFDKISNMEGVYSLDSFLKNKG 136
            NVN L ++QLGLTL+DE G +          W+FNF++F+ IS+M  + S++  L+   
Sbjct: 77  ANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFREFNVISDMFALDSIE-LLRKSA 136

Query: 137 FNFNILRSCGISPEEFSRQF--NPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMS 196
            +      CG+  + F+     + ++ + +I  W+TFH  YD  YL+K  +   +P  +S
Sbjct: 137 IDLEKNNECGVDAKRFAELLMGSGVVLNDKI-HWVTFHCGYDFGYLLKLLSGKELPEEIS 196

Query: 197 NFAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTAS 256
           +F          V D+K++  +   L     GL+K+A++L V R+G +H AGSDS LT  
Sbjct: 197 DFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAELLGVKRVGISHQAGSDSLLTLR 256

Query: 257 VYAKMRVIMKVDPKI-HEGFLYGLPYP 266
            + KM+        + + GFL+GL  P
Sbjct: 257 TFIKMKEFFFTGSLLKYSGFLFGLDNP 278

[Close]

BLAST of CmaCh19G001660 vs. ExPASy TrEMBL
Match: A0A6J1HXB4 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111467089 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.3e-165
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60
           MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD
Sbjct: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60

Query: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120
           EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK
Sbjct: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120

Query: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180
           GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN
Sbjct: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180

Query: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV 240
           FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV
Sbjct: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV 240

Query: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 286
           YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI
Sbjct: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 285

[Close]

BLAST of CmaCh19G001660 vs. ExPASy TrEMBL
Match: A0A6J1HSR3 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111467090 PE=3 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 2.4e-164
Identity = 283/285 (99.30%), Postives = 283/285 (99.30%), Query Frame = 0


Query: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60
           MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD
Sbjct: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60

Query: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120
           EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK
Sbjct: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120

Query: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180
           GFNFNILRSCGISPEEF RQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN
Sbjct: 121 GFNFNILRSCGISPEEFRRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180

Query: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV 240
           FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDS LTASV
Sbjct: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSLLTASV 240

Query: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 286
           YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI
Sbjct: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 285

[Close]

BLAST of CmaCh19G001660 vs. ExPASy TrEMBL
Match: A0A6J1HRD6 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111467091 PE=3 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 3.5e-163
Identity = 282/285 (98.95%), Postives = 282/285 (98.95%), Query Frame = 0


Query: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60
           MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFL CTPKGVSD
Sbjct: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLTCTPKGVSD 60

Query: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120
           EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK
Sbjct: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120

Query: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180
           GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN
Sbjct: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180

Query: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV 240
           FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDS LTASV
Sbjct: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSLLTASV 240

Query: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 286
           YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRP VVIVDPKLTHNRI
Sbjct: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPRVVIVDPKLTHNRI 285

[Close]

BLAST of CmaCh19G001660 vs. ExPASy TrEMBL
Match: A0A6J1IIN1 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111474351 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.0e-162
Identity = 280/285 (98.25%), Postives = 282/285 (98.95%), Query Frame = 0


Query: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60
           MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD
Sbjct: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60

Query: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120
           EEFYQCLQFNVN+LSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK
Sbjct: 61  EEFYQCLQFNVNQLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120

Query: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180
           GFNFNILRSCGISPEEF RQFNPILRSGRIQRWITFHGLYDIAYLMKFT+IICMPTSMS 
Sbjct: 121 GFNFNILRSCGISPEEFRRQFNPILRSGRIQRWITFHGLYDIAYLMKFTSIICMPTSMSI 180

Query: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV 240
           FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDS LTASV
Sbjct: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSLLTASV 240

Query: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 286
           YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI
Sbjct: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 285

[Close]

BLAST of CmaCh19G001660 vs. ExPASy TrEMBL
Match: A0A6J1ESM7 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 7.9e-147
Identity = 254/286 (88.81%), Postives = 273/286 (95.45%), Query Frame = 0


Query: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60
           MAKII  AAIVGG+WKR+VWSHNLLEEIELMKH LSEFPIVA+DTEFPGFLR TPKGVSD
Sbjct: 1   MAKIIEGAAIVGGQWKREVWSHNLLEEIELMKHYLSEFPIVAIDTEFPGFLRWTPKGVSD 60

Query: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120
           EEFYQCLQFNVNRLSILQLGLTLTD+NG+IGMTWEFNFQDFD+ISNMEGVYSLDSFLKNK
Sbjct: 61  EEFYQCLQFNVNRLSILQLGLTLTDDNGKIGMTWEFNFQDFDQISNMEGVYSLDSFLKNK 120

Query: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180
           GFNFN LRSCGI+P+EFSRQF P++RSGRIQ+WITFHGLYDIAYLMKF NII MPTSMS 
Sbjct: 121 GFNFNKLRSCGIAPQEFSRQFKPVIRSGRIQKWITFHGLYDIAYLMKFNNIISMPTSMSM 180

Query: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV 240
           FA++AAHLLGTVTDLKHMARYSDYL NGNLGLKKLAK+LQ+NR+GTAHFAGSDS LTASV
Sbjct: 181 FAMIAAHLLGTVTDLKHMARYSDYLFNGNLGLKKLAKLLQINRVGTAHFAGSDSLLTASV 240

Query: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDP-KLTHNRI 286
           YAKM+VIMKV+PKIHEGFLYGLPYPIQGYRPPVVIVDP KLT +RI
Sbjct: 241 YAKMKVIMKVEPKIHEGFLYGLPYPIQGYRPPVVIVDPSKLTDDRI 286

[Close]

BLAST of CmaCh19G001660 vs. NCBI nr
Match: XP_022967644.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima])

HSP 1 Score: 592.0 bits (1525), Expect = 2.7e-165
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60
           MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD
Sbjct: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60

Query: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120
           EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK
Sbjct: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120

Query: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180
           GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN
Sbjct: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180

Query: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV 240
           FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV
Sbjct: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV 240

Query: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 286
           YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI
Sbjct: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 285

[Close]

BLAST of CmaCh19G001660 vs. NCBI nr
Match: XP_022967646.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima])

HSP 1 Score: 587.8 bits (1514), Expect = 5.0e-164
Identity = 283/285 (99.30%), Postives = 283/285 (99.30%), Query Frame = 0


Query: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60
           MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD
Sbjct: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60

Query: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120
           EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK
Sbjct: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120

Query: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180
           GFNFNILRSCGISPEEF RQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN
Sbjct: 121 GFNFNILRSCGISPEEFRRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180

Query: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV 240
           FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDS LTASV
Sbjct: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSLLTASV 240

Query: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 286
           YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI
Sbjct: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 285

[Close]

BLAST of CmaCh19G001660 vs. NCBI nr
Match: XP_022967647.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima])

HSP 1 Score: 583.9 bits (1504), Expect = 7.3e-163
Identity = 282/285 (98.95%), Postives = 282/285 (98.95%), Query Frame = 0


Query: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60
           MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFL CTPKGVSD
Sbjct: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLTCTPKGVSD 60

Query: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120
           EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK
Sbjct: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120

Query: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180
           GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN
Sbjct: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180

Query: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV 240
           FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDS LTASV
Sbjct: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSLLTASV 240

Query: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 286
           YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRP VVIVDPKLTHNRI
Sbjct: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPRVVIVDPKLTHNRI 285

[Close]

BLAST of CmaCh19G001660 vs. NCBI nr
Match: XP_022975238.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima])

HSP 1 Score: 580.9 bits (1496), Expect = 6.1e-162
Identity = 280/285 (98.25%), Postives = 282/285 (98.95%), Query Frame = 0


Query: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60
           MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD
Sbjct: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60

Query: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120
           EEFYQCLQFNVN+LSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK
Sbjct: 61  EEFYQCLQFNVNQLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120

Query: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180
           GFNFNILRSCGISPEEF RQFNPILRSGRIQRWITFHGLYDIAYLMKFT+IICMPTSMS 
Sbjct: 121 GFNFNILRSCGISPEEFRRQFNPILRSGRIQRWITFHGLYDIAYLMKFTSIICMPTSMSI 180

Query: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV 240
           FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDS LTASV
Sbjct: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSLLTASV 240

Query: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 286
           YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI
Sbjct: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLTHNRI 285

[Close]

BLAST of CmaCh19G001660 vs. NCBI nr
Match: XP_022928830.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 529.6 bits (1363), Expect = 1.6e-146
Identity = 254/286 (88.81%), Postives = 273/286 (95.45%), Query Frame = 0


Query: 1   MAKIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSD 60
           MAKII  AAIVGG+WKR+VWSHNLLEEIELMKH LSEFPIVA+DTEFPGFLR TPKGVSD
Sbjct: 1   MAKIIEGAAIVGGQWKREVWSHNLLEEIELMKHYLSEFPIVAIDTEFPGFLRWTPKGVSD 60

Query: 61  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 120
           EEFYQCLQFNVNRLSILQLGLTLTD+NG+IGMTWEFNFQDFD+ISNMEGVYSLDSFLKNK
Sbjct: 61  EEFYQCLQFNVNRLSILQLGLTLTDDNGKIGMTWEFNFQDFDQISNMEGVYSLDSFLKNK 120

Query: 121 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 180
           GFNFN LRSCGI+P+EFSRQF P++RSGRIQ+WITFHGLYDIAYLMKF NII MPTSMS 
Sbjct: 121 GFNFNKLRSCGIAPQEFSRQFKPVIRSGRIQKWITFHGLYDIAYLMKFNNIISMPTSMSM 180

Query: 181 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASV 240
           FA++AAHLLGTVTDLKHMARYSDYL NGNLGLKKLAK+LQ+NR+GTAHFAGSDS LTASV
Sbjct: 181 FAMIAAHLLGTVTDLKHMARYSDYLFNGNLGLKKLAKLLQINRVGTAHFAGSDSLLTASV 240

Query: 241 YAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDP-KLTHNRI 286
           YAKM+VIMKV+PKIHEGFLYGLPYPIQGYRPPVVIVDP KLT +RI
Sbjct: 241 YAKMKVIMKVEPKIHEGFLYGLPYPIQGYRPPVVIVDPSKLTDDRI 286

[Close]

BLAST of CmaCh19G001660 vs. TAIR 10
Match: AT3G44240.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 164.9 bits (416), Expect = 9.8e-41
Identity = 95/220 (43.18%), Postives = 132/220 (60.00%), Query Frame = 0


Query: 28  IELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSDEEFYQCLQFNVNRLSILQLGLTLTDEN 87
           + L++ CL  +  +A+DTEFP  LR T +  +DEE Y  + F+V+R  ++QLGLTL D N
Sbjct: 1   MSLIEDCLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDIN 60

Query: 88  GRIGMTWEFNFQDF--DKISNMEGVYSLDSFLKNKGFNFNILRSCGISPEE-FSRQFNPI 147
           GRIG TWE NF DF  D   N + +     FL+  G +   +R  GI  E  FS  F  +
Sbjct: 61  GRIGGTWEINFSDFGVDDARNEKSI----EFLRRNGLDLRKIREEGIRIEGFFSEMFWML 120

Query: 148 LRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSNFAIMAAHLLGTVTDLKHMARYSDY 207
            ++ R   W+TFHG YDIAYL+K      +P +   F+   A +LG+V DLK MA   + 
Sbjct: 121 KKTRRNITWVTFHGSYDIAYLLKGFTGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEG 180

Query: 208 LLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASVYAKM 245
            L+  LGL+ LA    +NR+GTAH AGS++ LTA V+AK+
Sbjct: 181 -LSSRLGLETLAHEFGLNRVGTAHHAGSNNELTAMVFAKV 215

[Close]

BLAST of CmaCh19G001660 vs. TAIR 10
Match: AT1G06450.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 161.4 bits (407), Expect = 1.1e-39
Identity = 90/250 (36.00%), Postives = 136/250 (54.40%), Query Frame = 0


Query: 17  RDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSDEEFYQCLQFNVNRLSI 76
           R VW  N+ EE+  M  CL  FP++A DTE+PG +  T    S +E Y+ ++ NV    +
Sbjct: 10  RRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVENTKL 69

Query: 77  LQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNKGFNFNILRSCGISPEE 136
           +Q G TL +  G IG  WE NF +F   S+     S++ FL+  G +   +R  G+  + 
Sbjct: 70  IQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIE-FLRRHGLDLQKIRDEGV--DM 129

Query: 137 FSRQFNP----ILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSNFAIMAAHLLGTV 196
           F   F P    + RS +   ++TF G YD AY +   N   +P +   FA     + G V
Sbjct: 130 FGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILNHGKLPETHGEFATEVVKVFGQV 189

Query: 197 TDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASVYAKMRVIMKVDP 256
            D K MA + +  L  +LGL KLA++LQ+ R+G AH AGSDS +TA V+ K++ + + D 
Sbjct: 190 YDTKVMAGFCEG-LGEHLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE-DS 249

Query: 257 KIHEGFLYGL 263
           +   G +YG+
Sbjct: 250 RFARGLIYGI 254

[Close]

BLAST of CmaCh19G001660 vs. TAIR 10
Match: AT3G44260.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 158.7 bits (400), Expect = 7.0e-39
Identity = 97/266 (36.47%), Postives = 149/266 (56.02%), Query Frame = 0


Query: 17  RDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFL-RCTPKGVSDEEFYQCLQFNVNRLS 76
           R+VW+ NL  E EL+   + ++P ++MDTEFPG + +   +  + ++ Y  L+ NV+ LS
Sbjct: 20  REVWAENLESEFELISEIIDDYPFISMDTEFPGVIFKSDLRFTNPDDLYTLLKANVDALS 79

Query: 77  ILQLGLTLTDENGRI---------GMTWEFNFQDFDKISNMEGVYSLDSFLKNKGFNFNI 136
           ++Q+GLTL+D NG +         G  WEFNF+DFD   +     S++  L+ +G +F  
Sbjct: 80  LIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNFRDFDVARDAHAPDSIE-LLRRQGIDFE- 139

Query: 137 LRSC--GISPEEFSRQFNPILRSGRI----QRWITFHGLYDIAYLMKFTNIICMPTSMSN 196
            R+C  G+  E F+     ++ SG +      W+TFH  YD  YLMK      +P ++  
Sbjct: 140 -RNCRDGVESERFAEL---MMSSGLVCNEEVSWVTFHSAYDFGYLMKILTRRELPGALGE 199

Query: 197 FAIMAAHLLG-TVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNR-IGTAHFAGSDSFLTA 256
           F  +   L G  V D+KHM ++ +  L G  GL ++A+ L+VNR +G  H AGSDS LT 
Sbjct: 200 FKRVMRVLFGERVYDVKHMMKFCERRLFG--GLDRVARTLEVNRAVGKCHQAGSDSLLTW 259

Query: 257 SVYAKMRVIMKVD--PKIHEGFLYGL 263
             + +MR +  V   P+ H G LYGL
Sbjct: 260 HAFQRMRDLYFVQDGPEKHAGVLYGL 277

[Close]

BLAST of CmaCh19G001660 vs. TAIR 10
Match: AT1G61470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 156.4 bits (394), Expect = 3.5e-38
Identity = 93/230 (40.43%), Postives = 136/230 (59.13%), Query Frame = 0


Query: 18  DVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCTPKGVSDEEFYQCLQFNVNRLSIL 77
           +VW  N   E+  ++ CL     +A+DTEFPG L+ TP   SDE  Y+ ++FNV+   ++
Sbjct: 4   EVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLI 63

Query: 78  QLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNKGFNFNILRSCGISPEEF 137
           QLGLTL  +   I  TWE N  DF++  +++   S+ +FLKN G + + +R  GI  EEF
Sbjct: 64  QLGLTLFGKG--ITKTWEINLSDFNESKSLKNDKSI-AFLKNNGLDLDKIREEGIGIEEF 123

Query: 138 SRQFNPIL--RSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSNFAIMAAHLLGT-VTD 197
             +F+ IL  + G++ RW+TF G YD AYL+K      +P +   F      LLG  V D
Sbjct: 124 FMEFSQILNEKHGKM-RWVTFQGSYDKAYLLKGLTRKPLPETSKEFDETVQQLLGRFVYD 183

Query: 198 LKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTASVYAKM 245
           +K MA      L+   GL+++A +LQ+ R+G AH AGSDS LTA V+ K+
Sbjct: 184 VKKMAGLCSG-LSSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKL 228

[Close]

BLAST of CmaCh19G001660 vs. TAIR 10
Match: AT1G15920.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 155.2 bits (391), Expect = 7.8e-38
Identity = 91/267 (34.08%), Postives = 145/267 (54.31%), Query Frame = 0


Query: 17  RDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCT--------PKGVSDEEFYQCLQ 76
           R+VW+HNL +E+ L++  + +FP VAMDTEFPG +  T        P  +  E  Y  L+
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYNYDTLK 76

Query: 77  FNVNRLSILQLGLTLTDENGRI-------GMTWEFNFQDFDKISNMEGVYSLDSFLKNKG 136
            NVN L ++QLGLTL+DE G +          W+FNF++F+ IS+M  + S++  L+   
Sbjct: 77  ANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFREFNVISDMFALDSIE-LLRKSA 136

Query: 137 FNFNILRSCGISPEEFSRQF--NPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMS 196
            +      CG+  + F+     + ++ + +I  W+TFH  YD  YL+K  +   +P  +S
Sbjct: 137 IDLEKNNECGVDAKRFAELLMGSGVVLNDKI-HWVTFHCGYDFGYLLKLLSGKELPEEIS 196

Query: 197 NFAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSFLTAS 256
           +F          V D+K++  +   L     GL+K+A++L V R+G +H AGSDS LT  
Sbjct: 197 DFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAELLGVKRVGISHQAGSDSLLTLR 256

Query: 257 VYAKMRVIMKVDPKI-HEGFLYGLPYP 266
            + KM+        + + GFL+GL  P
Sbjct: 257 TFIKMKEFFFTGSLLKYSGFLFGLDNP 278

The following BLAST results are available for this feature:

BLAST of CmaCh19G001660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LXM4	1.4e-39	43.18	Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SHJ0	1.5e-38	36.00	Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9LXM2	9.9e-38	36.47	Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
O64773	4.9e-37	40.43	Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9S9P2	1.1e-36	34.08	Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]

BLAST of CmaCh19G001660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HXB4	1.3e-165	100.00	Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111467089 PE=3 S...	[more]
A0A6J1HSR3	2.4e-164	99.30	Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111467090 PE=3 S...	[more]
A0A6J1HRD6	3.5e-163	98.95	Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111467091 PE=3 S...	[more]
A0A6J1IIN1	3.0e-162	98.25	Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111474351 PE=3 S...	[more]
A0A6J1ESM7	7.9e-147	88.81	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3...	[more]

BLAST of CmaCh19G001660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022967644.1	2.7e-165	100.00	putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima]	[more]
XP_022967646.1	5.0e-164	99.30	putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima]	[more]
XP_022967647.1	7.3e-163	98.95	putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima]	[more]
XP_022975238.1	6.1e-162	98.25	putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima]	[more]
XP_022928830.1	1.6e-146	88.81	putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata]	[more]

BLAST of CmaCh19G001660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G44240.1	9.8e-41	43.18	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G06450.1	1.1e-39	36.00	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G44260.1	7.0e-39	36.47	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G61470.1	3.5e-38	40.43	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G15920.2	7.8e-38	34.08	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006941	Ribonuclease CAF1	PFAM	PF04857	CAF1	coord: 19..133 e-value: 2.8E-7 score: 30.1
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 15..264 e-value: 4.7E-64 score: 218.0
None	No IPR available	PANTHER	PTHR10797:SF57	CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATED	coord: 16..270
IPR039637	CCR4-NOT transcription complex subunit 7/8/Pop2	PANTHER	PTHR10797	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT	coord: 16..270
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 15..264

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh19G001660.1	CmaCh19G001660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006402	mRNA catabolic process
biological_process	GO:0090503	RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component	GO:0030014	CCR4-NOT complex
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004535	poly(A)-specific ribonuclease activity