CmaCh18G010210 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh18G010210
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionHomocysteine S-methyltransferase family protein
LocationCma_Chr18: 8522994 .. 8525553 (-)
RNA-Seq ExpressionCmaCh18G010210
SyntenyCmaCh18G010210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAATTGGATTTGGGTTTCAATCTCCACCAACCCCCGAGGGCTACAAAGGAGGGAAATTGGCGCGCAGAAGTTTTAGAAAGCGAGAATCTGAGAGCAAAGGCGTCCAGGTTCAGACTCGAGAGACATCGCGACAATATCCATATTCGGATTGTCACGCAATCCATTTCTCCTTGATCGACGGGGGTAATTGCGTATTGAAGATCGAAGGAACGAAGAGGAAGAAGAAGAAGATGGGGATGAAGAAGGCGATGACATTGTTGGATGATCTGATACAGAACGCAGGAGGTTGTGCGGTCATTGATGGAGGTTTGGCCACGCAGCTTGAGAAACACGGCGCTGTCTTTAACGATTCACTTTGGAGTGCTGTTTGCCTCGTTAAAGATCCCGACCTAATTAAGAAGGTTCTCTCTCACCGTCTCTGTTTTTTTTTTTTTTGTTCCTCTTCTTTTTGTTTTGTTGTTGGTGCATCACGATCTCGTCGGAGATTATTGATTCGATTGCATTTGTAGGTTCATTTGGAGTATTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCCTCTTATCAGGTTTGTACTGCGAATGGATTGAGCTTTCTGTACTGTTTTATCGATTGTAAACATGATAGTTTTGTACAATGCACTTTATGACGGATTTTGCAGTACCTTTTTCTTTAAAACGTTGTACTGTGGTTTGTTTAGACATCGATGATGTTATTTAGTTATTTTAAGGCTAATTAGCAGAATGCATGGGGTTCAAAACTAGCATTTCTATCCTGCCGTGAAATGATGAACCAAAATGGAACTGGTATTACCAGTATTCACTTACTCTTTCTTGTCATGCATTACTAACAATATGTACCAGTATTCACTTACTCTTTCTTGTTATGCATTTTATTCTATTCAGGCCACCATTCCTGGGTTTTTATCAAAGGGTCTGTCAGCAGAAGAAGGGGAGTTGCTCTTAGAAAGGAGTGTTAAATTGGCCGTGGAAGCTCGGGATAGCTTCTGGGATTCTGTCAAGAGTAACCCAGGGCATAATTACAATAGGGCTTTGGTTGCAGCATCCATAGGAAGCTATGGTGCTTATCTTGCTGATGGCTCGGAGTACAGGTGACTGACACATTGAATATTGAAATAAGGAGTTTGTAGATGTATGGCTTTCGAACTAAATATATTACCCCCACCCAATCAGTGGGCATTATGGACCAGATGTGGATGTGGATACGCTGAAGGATTTTCATCGACGCCGATTGCAGATTCTTGTCGGTGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTTGAAGCCCAGGTTTGTTTGTTGTTAACACTTGTTATATCATCTAAACTGTGGTTTAATAATACTAGACTCACTCTAATGCCGTCCTGATGATTTGACTGCAACTTATATTCTTATGGTTTGATCTAAGTCATAAAAGATCTGAGGAAGCAAATTAAGTATATTTTTAAATCTGTTCGTCTGAATTTACAAGTTGTTCATTTCAAGGGATCAAGACAAATTGACAATCGGGCATGGCATCGCCACGCCAATGGGGCATCCATTGCATGATCTGGTCTCAAATCGTTAACATCTGAATTTTTTGTCCTCTTTATGATGAATATCTTTTTATAATTTAAATGTGGTTTAAACCCTTTGTTTTAAAACTTATTCCAATTTGCTTATTTTACTTTCAGATATTCTATTTAATTCTCAAACTAGAATTACAACACTTCATTTAGTTGGTCCCGATTGTCACAACATACTGATGTTGAATGGTTTTTTTTTTTTTTTTTGGCTGTTGTGAGCATGTGAGTACATTTGTTTCAATTGATTTTCCAATTTTCATTATTTTCTCTCCTCTTGGTAATCTTGTTTCCAACCAAACTTACCTCTATTCCCCCCTCCCTTTCTGATCTAACTCAAAAAATTAGGCCTGTGTGGAATTGCTCGAAGAAGAAAACGTCCAAATTCTATCTTGGATCTGCTTTAGCTCCGTAGATGGTGAGAATGCCCCATCTGGAGAGAGCTTTGAGGAGTGTCTTCAGGCTATTAATAAAAGTGACAAAGTAAATGCAGTTGGAATAAACTGTACACCTCCTCATTTTATTGAAGCTCTCATTTGTAAGTTCAAAGAGGTAGGAACCAAATCTCTCACAGCTGTGATTTGATATTGTTTTCGTGTTCACTGGTTTTGACACCATAATATTCCCTTCAGTTAACAGACAAGCCCATAGTTGTTTATCCTAATAGTGGCGAGGTATGGGATGGTAGAGCCAAGAAATGGCTGGTGAGTCAAATGATTTTCCCCACCCACACTTTGATATTTTCATGGCTTGTGGGCCTACATTGTGATATTTCCAGAAGACTATAACTAATGTAATGCTCTGTTTTTTTGGCAGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCACTTAGTGCAAGATGGCGTGATTTGGGAGCCAAATTCATTGGTGGCTGTTGTCGAACAACACCATCTACCATCCGAGCGGTATCAAAGGTTTTGAAAGAAAGCCCTCATTAG

mRNA sequence

ATGGGAATTGGATTTGGGTTTCAATCTCCACCAACCCCCGAGGGCTACAAAGGAGGGAAATTGGCGCGCAGAAGTTTTAGAAAGCGAGAATCTGAGAGCAAAGGCGTCCAGGTTCAGACTCGAGAGACATCGCGACAATATCCATATTCGGATTGTCACGCAATCCATTTCTCCTTGATCGACGGGGGTAATTGCGTATTGAAGATCGAAGGAACGAAGAGGAAGAAGAAGAAGATGGGGATGAAGAAGGCGATGACATTGTTGGATGATCTGATACAGAACGCAGGAGGTTGTGCGGTCATTGATGGAGGTTTGGCCACGCAGCTTGAGAAACACGGCGCTGTCTTTAACGATTCACTTTGGAGTGCTGTTTGCCTCGTTAAAGATCCCGACCTAATTAAGAAGGTTCATTTGGAGTATTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCCTCTTATCAGGCCACCATTCCTGGGTTTTTATCAAAGGGTCTGTCAGCAGAAGAAGGGGAGTTGCTCTTAGAAAGGAGTGTTAAATTGGCCGTGGAAGCTCGGGATAGCTTCTGGGATTCTGTCAAGAGTAACCCAGGGCATAATTACAATAGGGCTTTGGTTGCAGCATCCATAGGAAGCTATGGTGCTTATCTTGCTGATGGCTCGGAGTACAGTGGGCATTATGGACCAGATGTGGATGTGGATACGCTGAAGGATTTTCATCGACGCCGATTGCAGATTCTTGTCGGTGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTTGAAGCCCAGGCCTGTGTGGAATTGCTCGAAGAAGAAAACGTCCAAATTCTATCTTGGATCTGCTTTAGCTCCGTAGATGGTGAGAATGCCCCATCTGGAGAGAGCTTTGAGGAGTGTCTTCAGGCTATTAATAAAAGTGACAAAGTAAATGCAGTTGGAATAAACTGTACACCTCCTCATTTTATTGAAGCTCTCATTTGTAAGTTCAAAGAGTTAACAGACAAGCCCATAGTTGTTTATCCTAATAGTGGCGAGGTATGGGATGGTAGAGCCAAGAAATGGCTGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCACTTAGTGCAAGATGGCGTGATTTGGGAGCCAAATTCATTGGTGGCTGTTGTCGAACAACACCATCTACCATCCGAGCGGTATCAAAGGTTTTGAAAGAAAGCCCTCATTAG

Coding sequence (CDS)

ATGGGAATTGGATTTGGGTTTCAATCTCCACCAACCCCCGAGGGCTACAAAGGAGGGAAATTGGCGCGCAGAAGTTTTAGAAAGCGAGAATCTGAGAGCAAAGGCGTCCAGGTTCAGACTCGAGAGACATCGCGACAATATCCATATTCGGATTGTCACGCAATCCATTTCTCCTTGATCGACGGGGGTAATTGCGTATTGAAGATCGAAGGAACGAAGAGGAAGAAGAAGAAGATGGGGATGAAGAAGGCGATGACATTGTTGGATGATCTGATACAGAACGCAGGAGGTTGTGCGGTCATTGATGGAGGTTTGGCCACGCAGCTTGAGAAACACGGCGCTGTCTTTAACGATTCACTTTGGAGTGCTGTTTGCCTCGTTAAAGATCCCGACCTAATTAAGAAGGTTCATTTGGAGTATTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCCTCTTATCAGGCCACCATTCCTGGGTTTTTATCAAAGGGTCTGTCAGCAGAAGAAGGGGAGTTGCTCTTAGAAAGGAGTGTTAAATTGGCCGTGGAAGCTCGGGATAGCTTCTGGGATTCTGTCAAGAGTAACCCAGGGCATAATTACAATAGGGCTTTGGTTGCAGCATCCATAGGAAGCTATGGTGCTTATCTTGCTGATGGCTCGGAGTACAGTGGGCATTATGGACCAGATGTGGATGTGGATACGCTGAAGGATTTTCATCGACGCCGATTGCAGATTCTTGTCGGTGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTTGAAGCCCAGGCCTGTGTGGAATTGCTCGAAGAAGAAAACGTCCAAATTCTATCTTGGATCTGCTTTAGCTCCGTAGATGGTGAGAATGCCCCATCTGGAGAGAGCTTTGAGGAGTGTCTTCAGGCTATTAATAAAAGTGACAAAGTAAATGCAGTTGGAATAAACTGTACACCTCCTCATTTTATTGAAGCTCTCATTTGTAAGTTCAAAGAGTTAACAGACAAGCCCATAGTTGTTTATCCTAATAGTGGCGAGGTATGGGATGGTAGAGCCAAGAAATGGCTGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCACTTAGTGCAAGATGGCGTGATTTGGGAGCCAAATTCATTGGTGGCTGTTGTCGAACAACACCATCTACCATCCGAGCGGTATCAAAGGTTTTGAAAGAAAGCCCTCATTAG

Protein sequence

MGIGFGFQSPPTPEGYKGGKLARRSFRKRESESKGVQVQTRETSRQYPYSDCHAIHFSLIDGGNCVLKIEGTKRKKKKMGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHLEYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGHNYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFETIPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGINCTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDLGAKFIGGCCRTTPSTIRAVSKVLKESPH
Homology
BLAST of CmaCh18G010210 vs. ExPASy Swiss-Prot
Match: A4ZGQ8 (Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN=HMT1 PE=1 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 5.7e-148
Identity = 245/324 (75.62%), Postives = 281/324 (86.73%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           MG++K   LL+DLI+  GGCAV+DGG ATQLE HGA  ND LWSAV L+KDP+LIK+VH+
Sbjct: 1   MGLEKKSALLEDLIEKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKDPELIKRVHM 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAGAD++V+SSYQATIPGFLS+GLS EE E LL++SVKLAVEARD FWD V    GH
Sbjct: 61  EYLEAGADVVVTSSYQATIPGFLSRGLSMEESESLLQKSVKLAVEARDRFWDKVSKTSGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
           +YNRALVAASIGSYGAYLADGSEYSG YG DV +D LKDFHRRR+Q+LV ASPDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGSYGEDVSLDKLKDFHRRRIQVLVEASPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEENVQI +WICF+SVDGENAPSGESF+ECL+ +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPAWICFTSVDGENAPSGESFQECLETLNKSNNICAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
           C PP F++ LI KF +LT K IVVYPNSGEVWDG+AKKWLPS CFGD +FE  + +WRDL
Sbjct: 241 CAPPQFMDNLIRKFSKLTQKAIVVYPNSGEVWDGKAKKWLPSQCFGDAEFEMFATKWRDL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLK 403
           GAK IGGCCRTTPSTI+A+S+ LK
Sbjct: 301 GAKLIGGCCRTTPSTIKAISRDLK 324

BLAST of CmaCh18G010210 vs. ExPASy Swiss-Prot
Match: Q9SDL7 (Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 5.9e-145
Identity = 243/324 (75.00%), Postives = 278/324 (85.80%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           M ++K   LL+DLI+  GGCAV+DGG ATQLE HGA  ND LWSAV L+K+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAGADI+V+SSYQATIPGFLS+GLS EE E LL++SV+LAVEARD FW+ V    GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V +D LKDFHRRRLQ+LV A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEE VQI +WICF+SVDGE APSGESFEECL+ +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
           C PP FIE LI KF +LT K IVVYPNSGEVWDG+AK+WLPS CFGDD+FE  + +WRDL
Sbjct: 241 CAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRDL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLK 403
           GAK IGGCCRTTPSTI A+S+ LK
Sbjct: 301 GAKLIGGCCRTTPSTINAISRDLK 324

BLAST of CmaCh18G010210 vs. ExPASy Swiss-Prot
Match: Q9FUN0 (Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 3.0e-128
Identity = 215/318 (67.61%), Postives = 253/318 (79.56%), Query Frame = 0

Query: 85  MTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHLEYLEAG 144
           M +L+DL+  AGGCAVIDGG ATQLE  GA  ND LWSA CL+  P L+K+VH++YLEAG
Sbjct: 1   MGVLEDLVARAGGCAVIDGGFATQLEALGADINDPLWSAACLITRPHLVKEVHMQYLEAG 60

Query: 145 ADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGHNYNRAL 204
           AD+++SSSYQATIPGF+++G+S  E E LL  SVKLA EARD FW S        YNRAL
Sbjct: 61  ADVIISSSYQATIPGFIARGMSVAEAEDLLRTSVKLANEARDEFWKSTLRKSKPIYNRAL 120

Query: 205 VAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFETIPNKLE 264
           VAASIGSYGAYLADGSEYSG YG D+  + LKDFHRRRLQ+L  A PDL+AFE IPN++E
Sbjct: 121 VAASIGSYGAYLADGSEYSGSYGADITAEKLKDFHRRRLQVLASAGPDLIAFEAIPNQME 180

Query: 265 AQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGINCTPPHF 324
           AQA VELLEEE VQI SWICFSSVDG+N  SGESF +CL+ +N S+KV  VG+NCTPP F
Sbjct: 181 AQALVELLEEEKVQIPSWICFSSVDGKNLCSGESFADCLKILNASEKVAVVGVNCTPPQF 240

Query: 325 IEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDLGAKFIG 384
           IE +IC+F++ T K I VYPNSGEVWDGRAK+WLP  C G   F++L+ RW++ GA  IG
Sbjct: 241 IEGIICEFRKQTKKAIAVYPNSGEVWDGRAKRWLPVECLGHKSFDALAKRWQEAGASLIG 300

Query: 385 GCCRTTPSTIRAVSKVLK 403
           GCCRTTPSTIRAVSK+LK
Sbjct: 301 GCCRTTPSTIRAVSKILK 318

BLAST of CmaCh18G010210 vs. ExPASy Swiss-Prot
Match: Q8LAX0 (Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 SV=2)

HSP 1 Score: 374.0 bits (959), Expect = 2.1e-102
Identity = 179/332 (53.92%), Postives = 235/332 (70.78%), Query Frame = 0

Query: 82  KKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHLEYL 141
           ++  +L+ D ++  GG AV+DGG AT+L++HGA  ND LWSA CL+  P L+ KVHL+YL
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYL 66

Query: 142 EAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSF--------WDSVK 201
           E+GA+I++++SYQATI GF++KGLS  E E LL RSV++  EAR+ F        WD   
Sbjct: 67  ESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDFAY 126

Query: 202 SNPGHNYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDL 261
           +    +    LVAAS+GSYGAYLADGSEYSG YG  V  +TLKDFHRRR+QIL  +  DL
Sbjct: 127 AGKA-SRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGADL 186

Query: 262 LAFETIPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVN 321
           +AFETIPNKLEA+A  +LLEEE++ I +W  F+S DG + P G+S  EC +  +    V 
Sbjct: 187 IAFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNVV 246

Query: 322 AVGINCTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSA 381
           A+GINCT P +I ALI   +++T KPIVVYPNSGEV+DG  KKW+ S    ++ F S  +
Sbjct: 247 AIGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYVS 306

Query: 382 RWRDLGAKFIGGCCRTTPSTIRAVSKVLKESP 406
           +WRD GA   GGCCRTTP+TIRA++KVL + P
Sbjct: 307 KWRDAGASLFGGCCRTTPNTIRAIAKVLSDEP 337

BLAST of CmaCh18G010210 vs. ExPASy Swiss-Prot
Match: Q9M1W4 (Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 5.2e-101
Identity = 178/319 (55.80%), Postives = 228/319 (71.47%), Query Frame = 0

Query: 88  LDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHLEYLEAGADI 147
           + D ++  GG AVIDGGLAT+ E+HGA  ND LWSA CLV  P LI  VHL+YLEAGADI
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 148 LVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGHN-----YNR 207
           + S+SYQATI GF +KG S EE E LL++SV++A EAR+S++D   ++   +        
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKKRP 128

Query: 208 ALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFETIPNK 267
            LVAAS+GSYGAYLADGSEYSG YG  + ++ LKDFHRRRLQ+L  +  DL+AFETIPNK
Sbjct: 129 ILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNK 188

Query: 268 LEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGINCTPP 327
           +EAQA  +LLEE +V+I  W  F+S DG N  SG+S +EC+      +KV AVGINCTPP
Sbjct: 189 IEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPP 248

Query: 328 HFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDLGAKF 387
            FIE L+ + +++T KPI+VYPNSGE +D   K+W+ +   GD+ F S   +W D G   
Sbjct: 249 RFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAGVSL 308

Query: 388 IGGCCRTTPSTIRAVSKVL 402
           +GGCCRTTP+TIRA+ K L
Sbjct: 309 LGGCCRTTPTTIRAIHKRL 327

BLAST of CmaCh18G010210 vs. ExPASy TrEMBL
Match: A0A6J1HZT4 (homocysteine S-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467714 PE=4 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 9.7e-191
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL
Sbjct: 1   MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLKESPH 407
           GAKFIGGCCRTTPSTIRAVSKVLKESPH
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

BLAST of CmaCh18G010210 vs. ExPASy TrEMBL
Match: A0A6J1G1A5 (homocysteine S-methyltransferase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449762 PE=4 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.4e-186
Identity = 321/328 (97.87%), Postives = 324/328 (98.78%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           MGMKKA TLLDDLIQNAGGC VIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCVVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAG+DILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH
Sbjct: 61  EYLEAGSDILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLK FHRRRLQILVGASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEENVQI SWICFSSVDGENAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLS+RWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLKESPH 407
           GAKFIGGCCRTTPSTIRAVSKVLKESPH
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

BLAST of CmaCh18G010210 vs. ExPASy TrEMBL
Match: A0A6J1D951 (homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC111018744 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.8e-174
Identity = 300/326 (92.02%), Postives = 312/326 (95.71%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           M MKKA TLLDDLI+NAGGCAVIDGG ATQLEKHGAV ND LWSAVCL+KDPDLIKKVHL
Sbjct: 1   MVMKKATTLLDDLIENAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLIKDPDLIKKVHL 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAGADI+VSSSYQATIPGFLSKGLS EEGELLLE+SVKLAVEARDSFW+SVKS PGH
Sbjct: 61  EYLEAGADIVVSSSYQATIPGFLSKGLSTEEGELLLEKSVKLAVEARDSFWNSVKSIPGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
            YNRALVAASIGSYGAYLADGSEYSG+YGPDV+VD LKDFHRRRLQ+LV A PDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGNYGPDVNVDKLKDFHRRRLQVLVNACPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEENVQI SWICFSSVDG+NAPSGESFEECL+AINKS+KVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGKNAPSGESFEECLRAINKSNKVNAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFES S+RWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESFSSRWRDL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLKES 405
           GAKFIGGCCRTTPST RAVSKVLKES
Sbjct: 301 GAKFIGGCCRTTPSTTRAVSKVLKES 326

BLAST of CmaCh18G010210 vs. ExPASy TrEMBL
Match: A0A1S3BEY1 (homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 2.2e-171
Identity = 295/328 (89.94%), Postives = 309/328 (94.21%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           MG++KA TLLDDL+ NAGGCAVIDGGLATQLEKHGAVFND LWSAVCL+ DPDLIKKVHL
Sbjct: 1   MGIEKATTLLDDLLHNAGGCAVIDGGLATQLEKHGAVFNDPLWSAVCLINDPDLIKKVHL 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAGADILV+SSYQATIPGF+SKGLS EEGELLLE+SVKLA+EARDSFWDSVK  PGH
Sbjct: 61  EYLEAGADILVTSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKCIPGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LKDFHRRRL+ILV ASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLRILVDASPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEENVQI SWICFSSVDGENAPSGESFE+CL AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
           CTPPHFIEALI KFKELT+K IVVYPNSGEVWDGR KKWLPSNCFGDDKFES ++RW  L
Sbjct: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRFKKWLPSNCFGDDKFESFTSRWCKL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLKESPH 407
           GA FIGGCCRTTPSTIRAVSKVLKES H
Sbjct: 301 GATFIGGCCRTTPSTIRAVSKVLKESSH 328

BLAST of CmaCh18G010210 vs. ExPASy TrEMBL
Match: A0A0A0KR42 (Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660 PE=4 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 5.5e-170
Identity = 293/326 (89.88%), Postives = 305/326 (93.56%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           MG+KKA TLLDD + NAGGCAVIDGG ATQLEKHGAV ND LWSAVCL+ DP LIKKVHL
Sbjct: 1   MGIKKATTLLDDFLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPHLIKKVHL 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAGADILVSSSYQATIPGF+SKGLS EEGELLLE+SVKLA+EARDSFWDSVK  PGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKCIPGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
            YNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LKDFHRRRLQI V ASPDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQIFVDASPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEEN+QI SWICFSSVDGENAPSGESFE+CL AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENIQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
           CTPPHFIEALI KFKELT+K IVVYPNSGEVWDGR KKWLPSNCFGDDKFESLS+RWR+L
Sbjct: 241 CTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRFKKWLPSNCFGDDKFESLSSRWRNL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLKES 405
           GA FIGGCCRTTPSTIRAVSKVLKES
Sbjct: 301 GATFIGGCCRTTPSTIRAVSKVLKES 326

BLAST of CmaCh18G010210 vs. NCBI nr
Match: XP_022968494.1 (homocysteine S-methyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 676.0 bits (1743), Expect = 2.0e-190
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL
Sbjct: 1   MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLKESPH 407
           GAKFIGGCCRTTPSTIRAVSKVLKESPH
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

BLAST of CmaCh18G010210 vs. NCBI nr
Match: XP_023541078.1 (homocysteine S-methyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 669.8 bits (1727), Expect = 1.4e-188
Identity = 325/328 (99.09%), Postives = 326/328 (99.39%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           MGMKKA TLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEENVQI SWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLS+RWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLKESPH 407
           GAKFIGGCCRTTPSTIRAVSKVLKESPH
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

BLAST of CmaCh18G010210 vs. NCBI nr
Match: KAG6573963.1 (Homocysteine S-methyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013023.1 Homocysteine S-methyltransferase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 662.5 bits (1708), Expect = 2.3e-186
Identity = 322/328 (98.17%), Postives = 325/328 (99.09%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           MGMKKA TLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPG+
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLK FHRRRLQILVGASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEENVQI SWICFSSVDGENAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLS+RWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLKESPH 407
           GAKFIGGCCRTTPSTIRAVSKVLKESPH
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

BLAST of CmaCh18G010210 vs. NCBI nr
Match: XP_022945567.1 (homocysteine S-methyltransferase 1 [Cucurbita moschata])

HSP 1 Score: 662.1 bits (1707), Expect = 3.0e-186
Identity = 321/328 (97.87%), Postives = 324/328 (98.78%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           MGMKKA TLLDDLIQNAGGC VIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCVVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAG+DILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH
Sbjct: 61  EYLEAGSDILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLK FHRRRLQILVGASPDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEENVQI SWICFSSVDGENAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLS+RWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLKESPH 407
           GAKFIGGCCRTTPSTIRAVSKVLKESPH
Sbjct: 301 GAKFIGGCCRTTPSTIRAVSKVLKESPH 328

BLAST of CmaCh18G010210 vs. NCBI nr
Match: XP_022150665.1 (homocysteine S-methyltransferase 1 [Momordica charantia])

HSP 1 Score: 621.3 bits (1601), Expect = 5.8e-174
Identity = 300/326 (92.02%), Postives = 312/326 (95.71%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           M MKKA TLLDDLI+NAGGCAVIDGG ATQLEKHGAV ND LWSAVCL+KDPDLIKKVHL
Sbjct: 1   MVMKKATTLLDDLIENAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLIKDPDLIKKVHL 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAGADI+VSSSYQATIPGFLSKGLS EEGELLLE+SVKLAVEARDSFW+SVKS PGH
Sbjct: 61  EYLEAGADIVVSSSYQATIPGFLSKGLSTEEGELLLEKSVKLAVEARDSFWNSVKSIPGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
            YNRALVAASIGSYGAYLADGSEYSG+YGPDV+VD LKDFHRRRLQ+LV A PDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGNYGPDVNVDKLKDFHRRRLQVLVNACPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEENVQI SWICFSSVDG+NAPSGESFEECL+AINKS+KVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGKNAPSGESFEECLRAINKSNKVNAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
           CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFES S+RWRDL
Sbjct: 241 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESFSSRWRDL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLKES 405
           GAKFIGGCCRTTPST RAVSKVLKES
Sbjct: 301 GAKFIGGCCRTTPSTTRAVSKVLKES 326

BLAST of CmaCh18G010210 vs. TAIR 10
Match: AT3G25900.1 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 515.4 bits (1326), Expect = 4.2e-146
Identity = 243/324 (75.00%), Postives = 278/324 (85.80%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           M ++K   LL+DLI+  GGCAV+DGG ATQLE HGA  ND LWSAV L+K+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAGADI+V+SSYQATIPGFLS+GLS EE E LL++SV+LAVEARD FW+ V    GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V +D LKDFHRRRLQ+LV A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEE VQI +WICF+SVDGE APSGESFEECL+ +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
           C PP FIE LI KF +LT K IVVYPNSGEVWDG+AK+WLPS CFGDD+FE  + +WRDL
Sbjct: 241 CAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRDL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLK 403
           GAK IGGCCRTTPSTI A+S+ LK
Sbjct: 301 GAKLIGGCCRTTPSTINAISRDLK 324

BLAST of CmaCh18G010210 vs. TAIR 10
Match: AT3G25900.3 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 474.6 bits (1220), Expect = 8.2e-134
Identity = 229/324 (70.68%), Postives = 263/324 (81.17%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           M ++K   LL+DLI+  GGCAV+DGG ATQLE HGA  ND LWSAV L+K+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAGADI+V+SSYQATIPGFLS+GLS EE E LL++SV+LAVEARD FW+ V    GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V +D LKDFHRRRLQ+LV A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEE VQI +WICF+SVDGE APSGESFEECL+ +NKS+ + A    
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYA---- 240

Query: 319 CTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDL 378
                           LT K IVVYPNSGEVWDG+AK+WLPS CFGDD+FE  + +WRDL
Sbjct: 241 ----------------LTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRDL 300

Query: 379 GAKFIGGCCRTTPSTIRAVSKVLK 403
           GAK IGGCCRTTPSTI A+S+ LK
Sbjct: 301 GAKLIGGCCRTTPSTINAISRDLK 304

BLAST of CmaCh18G010210 vs. TAIR 10
Match: AT3G25900.2 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 401.4 bits (1030), Expect = 8.8e-112
Identity = 193/260 (74.23%), Postives = 222/260 (85.38%), Query Frame = 0

Query: 79  MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 138
           M ++K   LL+DLI+  GGCAV+DGG ATQLE HGA  ND LWSAV L+K+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 139 EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 198
           EYLEAGADI+V+SSYQATIPGFLS+GLS EE E LL++SV+LAVEARD FW+ V    GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 199 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 258
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V +D LKDFHRRRLQ+LV A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 259 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 318
           IPNKLEAQACVELLEEE VQI +WICF+SVDGE APSGESFEECL+ +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 319 CTPPHFIEALICKFKELTDK 339
           C PP FIE LI KF + TD+
Sbjct: 241 CAPPQFIENLIRKFAKATDE 260

BLAST of CmaCh18G010210 vs. TAIR 10
Match: AT3G22740.1 (homocysteine S-methyltransferase 3 )

HSP 1 Score: 374.0 bits (959), Expect = 1.5e-103
Identity = 179/332 (53.92%), Postives = 235/332 (70.78%), Query Frame = 0

Query: 82  KKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHLEYL 141
           ++  +L+ D ++  GG AV+DGG AT+L++HGA  ND LWSA CL+  P L+ KVHL+YL
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYL 66

Query: 142 EAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSF--------WDSVK 201
           E+GA+I++++SYQATI GF++KGLS  E E LL RSV++  EAR+ F        WD   
Sbjct: 67  ESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDFAY 126

Query: 202 SNPGHNYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDL 261
           +    +    LVAAS+GSYGAYLADGSEYSG YG  V  +TLKDFHRRR+QIL  +  DL
Sbjct: 127 AGKA-SRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGADL 186

Query: 262 LAFETIPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVN 321
           +AFETIPNKLEA+A  +LLEEE++ I +W  F+S DG + P G+S  EC +  +    V 
Sbjct: 187 IAFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNVV 246

Query: 322 AVGINCTPPHFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSA 381
           A+GINCT P +I ALI   +++T KPIVVYPNSGEV+DG  KKW+ S    ++ F S  +
Sbjct: 247 AIGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYVS 306

Query: 382 RWRDLGAKFIGGCCRTTPSTIRAVSKVLKESP 406
           +WRD GA   GGCCRTTP+TIRA++KVL + P
Sbjct: 307 KWRDAGASLFGGCCRTTPNTIRAIAKVLSDEP 337

BLAST of CmaCh18G010210 vs. TAIR 10
Match: AT3G63250.1 (homocysteine methyltransferase 2 )

HSP 1 Score: 369.4 bits (947), Expect = 3.7e-102
Identity = 178/319 (55.80%), Postives = 228/319 (71.47%), Query Frame = 0

Query: 88  LDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHLEYLEAGADI 147
           + D ++  GG AVIDGGLAT+ E+HGA  ND LWSA CLV  P LI  VHL+YLEAGADI
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 148 LVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGHN-----YNR 207
           + S+SYQATI GF +KG S EE E LL++SV++A EAR+S++D   ++   +        
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKKRP 128

Query: 208 ALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFETIPNK 267
            LVAAS+GSYGAYLADGSEYSG YG  + ++ LKDFHRRRLQ+L  +  DL+AFETIPNK
Sbjct: 129 ILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNK 188

Query: 268 LEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGINCTPP 327
           +EAQA  +LLEE +V+I  W  F+S DG N  SG+S +EC+      +KV AVGINCTPP
Sbjct: 189 IEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPP 248

Query: 328 HFIEALICKFKELTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSARWRDLGAKF 387
            FIE L+ + +++T KPI+VYPNSGE +D   K+W+ +   GD+ F S   +W D G   
Sbjct: 249 RFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAGVSL 308

Query: 388 IGGCCRTTPSTIRAVSKVL 402
           +GGCCRTTP+TIRA+ K L
Sbjct: 309 LGGCCRTTPTTIRAIHKRL 327

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A4ZGQ85.7e-14875.62Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN... [more]
Q9SDL75.9e-14575.00Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1... [more]
Q9FUN03.0e-12867.61Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1[more]
Q8LAX02.1e-10253.92Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 ... [more]
Q9M1W45.2e-10155.80Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1HZT49.7e-191100.00homocysteine S-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467... [more]
A0A6J1G1A51.4e-18697.87homocysteine S-methyltransferase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449762... [more]
A0A6J1D9512.8e-17492.02homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC11101874... [more]
A0A1S3BEY12.2e-17189.94homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 ... [more]
A0A0A0KR425.5e-17089.88Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660... [more]
Match NameE-valueIdentityDescription
XP_022968494.12.0e-190100.00homocysteine S-methyltransferase 1-like [Cucurbita maxima][more]
XP_023541078.11.4e-18899.09homocysteine S-methyltransferase 1-like [Cucurbita pepo subsp. pepo][more]
KAG6573963.12.3e-18698.17Homocysteine S-methyltransferase 1, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022945567.13.0e-18697.87homocysteine S-methyltransferase 1 [Cucurbita moschata][more]
XP_022150665.15.8e-17492.02homocysteine S-methyltransferase 1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G25900.14.2e-14675.00Homocysteine S-methyltransferase family protein [more]
AT3G25900.38.2e-13470.68Homocysteine S-methyltransferase family protein [more]
AT3G25900.28.8e-11274.23Homocysteine S-methyltransferase family protein [more]
AT3G22740.11.5e-10353.92homocysteine S-methyltransferase 3 [more]
AT3G63250.13.7e-10255.80homocysteine methyltransferase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003726Homocysteine-binding domainPFAMPF02574S-methyl_transcoord: 100..400
e-value: 3.0E-72
score: 243.8
IPR003726Homocysteine-binding domainPROSITEPS50970HCYcoord: 87..401
score: 48.079597
IPR036589Homocysteine-binding domain superfamilyGENE3D3.20.20.330coord: 88..399
e-value: 1.3E-96
score: 325.6
IPR036589Homocysteine-binding domain superfamilySUPERFAMILY82282Homocysteine S-methyltransferasecoord: 95..403
NoneNo IPR availablePIRSRPIRSR037505-2PIRSR037505-2coord: 87..320
e-value: 2.3E-9
score: 34.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..42
NoneNo IPR availablePANTHERPTHR46015:SF7HOMOCYSTEINE S-METHYLTRANSFERASE 1coord: 79..403
NoneNo IPR availablePANTHERPTHR46015ZGC:172121coord: 79..403

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G010210.1CmaCh18G010210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009086 methionine biosynthetic process
biological_process GO:0032259 methylation
biological_process GO:0033528 S-methylmethionine cycle
molecular_function GO:0047150 betaine-homocysteine S-methyltransferase activity
molecular_function GO:0008898 S-adenosylmethionine-homocysteine S-methyltransferase activity
molecular_function GO:0008270 zinc ion binding