CmaCh18G009490 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh18G009490
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptioniron-sulfur assembly protein IscA-like 1, mitochondrial
LocationCma_Chr18: 8106611 .. 8110369 (-)
RNA-Seq ExpressionCmaCh18G009490
SyntenyCmaCh18G009490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGCTTCTTCGTCTTCCTTCGTCGCCACAGCTTCTCAGAAGGTCGGACCAGCTGCTCGCCGCCAAATTCTTACTCTGACGGAGGCCGCTGCCGCTAGAATTCGACAACTTCTTCAACAGCGCCAGCGTTCATTCCTTCGCCTAGGCGTCAAGTCTCGCGGCTGCAATGGCTTGTCCTATACTCTCAACTATGCAGGTTTGTAATACCTATTGATTTCAAACTTTTATCGATTAGATGCAGCTTCTTTTTAATCATTATACACGTCTGAATTTGGCGGATGTTGATGCTTGGATTTAATACGAGATAAATAGGTATTTGTTGTATTTGAGAGATATAACCTAATTGTTTAGAGGTGACGTGGAGAAACGGAGTCCTCTAGAAGGTGAATTCTTGATATTTAAACTGTTTCCAGCTCTAGCTACCATGAATTACGATGTTAGGCCGATGATTCATCCTGATGATTAGATGTCAAAAAGTTGGAAGTTTAAATGAACCTGTAATTGTGATTTACCGGAATTGGACCTAGCTTTGATATATAACTTGTTCGAAGATGAGCTGTTGAATTATTGCTAATCTTAACAGACGACAAATATGAAGATAAAGCTGACATGCCTGAATGCTGAAGTCTACCGATTAGAGATGACTTTATAGGTTGGGATTGTTAGGGACTCTCACGTTGACTAATTAAGGGGAAGATTATGGTTATATAAGTAAGAGACCCTATCTCAATTGGTACGAGGTTTTTTGGTAAAATCAAAAGTAAAGTTATGAGAGCTTATGCTCAAAGTGGACAATATCATATTATTATGGAGATATGTTGTTCCTATCAAAGACTATGTATGAGATAACTAATAGTTCAGACTTTAAATATATCCGTCCAAGCCCACCGCTAGCAGATGTTGTCCTATTTGAGTTTTCTTTTCTGGGCTTCCCCTCAAGGTTTTAAAACGCGTCTACTAGGGAGAGGTTTTCATGCCTTTATAGGAATGCTTCATTCCTCTCTCCAACCGATGTGGAATCTCACAATCCACCCCTTTAGTGTGCCAGAAAGGCTCGGTGTTTGCCTGTGATTCCATTTGTGACAACTCAAGCCCATTGCTAACAACTATTATCCTCTTTGCCCTTATGGGCTTCAAGATTTTGAAACGCGTCTACTAGGGAGAGGTTTTCACACCTTTATAAGGAATGGTTCGTTCCCCTATTCAACCGACATGGGATCTCACGTTAAATGAGTTATGTGACAGGAAGTTTAGATTCAATAATTTACTTCACAAACTTATGAGAGATGGATTAGTGGGTTATTGAACATATCACAACCCCAGACTAATCTATGGTGAAGATTTACTTCACGAACTTATGAGAGATGGATTAGTTATTAACATGATAGATGGCCAGAAGTTTTCGATTTCAACAATTTTGTTGTAACAACCCAAGCCCACCTCTATTAGATATTGTCCATTTTGACCCATGACGTATCGTCATCGGCCTCACGGTTTTAAAACGCGTATGCTAGGGAGAAGTTTCCACACCTTTATAAAGAATATTTCGTTCTCCTCTCCAACCGACGTGGGATCTCAAACCTGTAGCTTGAGACTTTTTTTATTGTTTGTGTTTATCTAATATTTCTATCCTCTCAGTGAACCGGATTTGCAATTCAGTTTCTTATGGATTCGCTCATGATTGGATAATTTTCTTACTCAGCATTACCTGAGTTTTATAATCACATTATCATTAATTTCCGTTCATTTTCAATGAACAAGAAATAGAATGCAGCCTTTATCTCTCCTTTTGGCACAAATGGACTGTCATGACCTCTATTTCTTTAAACAGTTCAAGATTGCAGTACTTCATTTTAAAGTTTTCTCTTTTCAAATACTTCAGATGAGAAGGGAAAATTTGATGAGCTGGTTGAGGATAAGGACGTGAAGATATTGGTTGATCCAAAGGCGCTGATGCATGTAATAGGAACAAAGATGGACTTTGTAGATGATAAACTGAGGTATTTGAGAACTCTCTACCACAATTTTATATTTTCAGCTCTATTTCTATCCATATGTTGAGGGAATATCCTGCTTCTTAGATACAACTTACAATATCAATCCGACATATAAAAGCTTGTGTGTTTATATTTAAATGAGTAAGCATGTGCATTTGTATGTAAACCTATTGTTCTACATTTATCACGGCACCTCGTGTGAGATCCCATATCGGTTGGAAAGGAAAACGAAGCATTTTGTCTAGAAGTGTGGAAACCACTCCCTAGTAGACGCGTTTTAAAAACTTCAAGGGGAAACTCGAAAGGGAAAGCCCAAAAAGGATAATATTTGCTATGGTGAGCTTGAGCTATTACAAATGGTATCAGAGCCAAGCACCAATGCTGGGCCTCGAAGGGGGATGAATTGTGAGATCCCACGTTAGTTGGACAGGAAAACTACACATTCATTTTAAAAACTTTAAAGGAAAATCCGAAAGGGAAAGCCCAAAGAGAACAAAATCTTCTAGAAGTGAGCTTTGGCGGTTACACCTCGGCAACGATATTTTATTATTTCAGAGTTTGGACATATTTCAATAGGTTTTAATCAGCTGTTTTGATTCTCAAATTTGTTAGGTTATCTCGAGTTTTGATTCTCGAATGAAATCCAACCTTTTAACCCCTGTAGTACTGCTGTATTCAGTTGTGAATGAAACTTAATTTTTTTTTCTGTCTCTGTCTTCTTGGTAGATCCGAGTTCATCTTTATCAATCCAAATTCTAAAGGACAATGTGGGTGTGGGGAGTCGTTTATGACAACAGGCAGTGCAAAGGCAGTTGAACAATGAGCAACTACACCAAGAGAGTGAAACAGTTGTAAGAATGTTTGTTTTATTTATGTCTAATTATATTCTTAAAACCCATTTAAGCAGCCCCTTGTGATCTGCTAATTAGTTTCTTTTGTTTTGTATGTACATATTGTTGAGTGCAGTGGCTAAATAAGTTCTCTGAGGTCAGTAATCAAATTCTCCCACTACTTTCCCTTGTTCTAGAATTTGGTGGTTGAGATCATAGGATAAGAGCACTCCCACTCCCTTCTTGTCAAATGTCTTCATATTTCATATCACTTATCACTTATTAAAGTATAATTCAACAGTTCGCTTATTGAGATCATAGCGAAAATGGTTTAACAATGACTTAAATAATTGAGATTGTTTATTATCACTTACGTTATTTTTTGTGATGTCCCAACACAAACATGTGGAAACCTTCCTCTAGCAGACGCGTTTAAAGCTTTGAGGGAAGTCCGAGAGGGAGAGTCCAAAGAGGACAATATTTGCTAGCGTGGGCTTGGGTTGTTACAAATGGTATCAGAACCAAACACCGGATGATGTGCTAGCCTTCTCGCTATTCCCCGAAGGGGTAGACATGAGGCGGTGTGCTAGTAAGGATGCTGGGCTCTATAGGGGGTGGATTTAGTGGGGCGTCCCACATCAATTCGAGAAATAAATGAGTGCCAGCGAGGACGTTAGGCCCCGAAGGGGGTGGATTATGATGTCCCACATTGTTTGGGGGAAAACGAAACATCATTTATAAGAGAGTTGAAACCTTCTCCTAACAAATGCGTTTTAAAGCCTTGAGGGGAAGTCTGAAAGCTCCCTTAAAGGAAAGTCCAAGGCGGACAATATCGGTTAGCGGTAGGCTTGGACAGAAAGATATGCATAAATAACTAATTTTTTTTGAAATTGGATCATATTAATAGTAAATTAATTTAGTTCTACAAA

mRNA sequence

ATGGCAGCTTCTTCGTCTTCCTTCGTCGCCACAGCTTCTCAGAAGGTCGGACCAGCTGCTCGCCGCCAAATTCTTACTCTGACGGAGGCCGCTGCCGCTAGAATTCGACAACTTCTTCAACAGCGCCAGCGTTCATTCCTTCGCCTAGGCGTCAAGTCTCGCGGCTGCAATGGCTTGTCCTATACTCTCAACTATGCAGATGAGAAGGGAAAATTTGATGAGCTGGTTGAGGATAAGGACGTGAAGATATTGGTTGATCCAAAGGCGCTGATGCATGTAATAGGAACAAAGATGGACTTTGTAGATGATAAACTGAGATCCGAGTTCATCTTTATCAATCCAAATTCTAAAGGACAATGTGGGTGTGGGGAGTCGTTTATGACAACAGGCAGTGCAAAGGCAGTTGAACAATGAGCAACTACACCAAGAGAGTGAAACAGTTTTTCTTTTGTTTTGTATGTACATATTGTTGAGTGCAGTGGCTAAATAAGTTCTCTGAGGTCAGTAATCAAATTCTCCCACTACTTTCCCTTGTTCTAGAATTTGGTGGTTGAGATCATAGGATAAGAGCACTCCCACTCCCTTCTTGTCAAATGTCTTCATATTTCATATCACTTATCACTTATTAAAGTATAATTCAACAGTTCGCTTATTGAGATCATAGCGAAAATGGTTTAACAATGACTTAAATAATTGAGATTGTTTATTATCACTTACGTTATTTTTTGTGATGTCCCAACACAAACATGTGGAAACCTTCCTCTAGCAGACGCGTTTAAAGCTTTGAGGGAAGTCCGAGAGGGAGAGTCCAAAGAGGACAATATTTGCTAGCGTGGGCTTGGGTTGTTACAAATGGTATCAGAACCAAACACCGGATGATGTGCTAGCCTTCTCGCTATTCCCCGAAGGGGTAGACATGAGGCGGTGTGCTAGTAAGGATGCTGGGCTCTATAGGGGGTGGATTTAGTGGGGCGTCCCACATCAATTCGAGAAATAAATGAGTGCCAGCGAGGACGTTAGGCCCCGAAGGGGGTGGATTATGATGTCCCACATTGTTTGGGGGAAAACGAAACATCATTTATAAGAGAGTTGAAACCTTCTCCTAACAAATGCGTTTTAAAGCCTTGAGGGGAAGTCTGAAAGCTCCCTTAAAGGAAAGTCCAAGGCGGACAATATCGGTTAGCGGTAGGCTTGGACAGAAAGATATGCATAAATAACTAATTTTTTTTGAAATTGGATCATATTAATAGTAAATTAATTTAGTTCTACAAA

Coding sequence (CDS)

ATGGCAGCTTCTTCGTCTTCCTTCGTCGCCACAGCTTCTCAGAAGGTCGGACCAGCTGCTCGCCGCCAAATTCTTACTCTGACGGAGGCCGCTGCCGCTAGAATTCGACAACTTCTTCAACAGCGCCAGCGTTCATTCCTTCGCCTAGGCGTCAAGTCTCGCGGCTGCAATGGCTTGTCCTATACTCTCAACTATGCAGATGAGAAGGGAAAATTTGATGAGCTGGTTGAGGATAAGGACGTGAAGATATTGGTTGATCCAAAGGCGCTGATGCATGTAATAGGAACAAAGATGGACTTTGTAGATGATAAACTGAGATCCGAGTTCATCTTTATCAATCCAAATTCTAAAGGACAATGTGGGTGTGGGGAGTCGTTTATGACAACAGGCAGTGCAAAGGCAGTTGAACAATGA

Protein sequence

MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLSYTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTGSAKAVEQ
Homology
BLAST of CmaCh18G009490 vs. ExPASy Swiss-Prot
Match: Q8LBM4 (Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g16710 PE=2 SV=2)

HSP 1 Score: 207.2 bits (526), Expect = 1.2e-52
Identity = 100/130 (76.92%), Postives = 118/130 (90.77%), Query Frame = 0

Query: 5   SSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLSYTLN 64
           +S  +A A+ +VGPA R+Q+LTLT+ AA+R+  LLQQRQ+ FLRLGVK+RGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGE 124
           YADEKGKFDELVE+K V+ILV+PKALMHVIGTKMDFVDDKLRSEF+FINPNS+GQCGCGE
Sbjct: 63  YADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGE 122

Query: 125 SFMTTGSAKA 135
           SFMTT ++ A
Sbjct: 123 SFMTTSTSSA 132

BLAST of CmaCh18G009490 vs. ExPASy Swiss-Prot
Match: Q8L8C0 (Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g36260 PE=3 SV=2)

HSP 1 Score: 169.9 bits (429), Expect = 2.1e-41
Identity = 81/109 (74.31%), Postives = 98/109 (89.91%), Query Frame = 0

Query: 21  RRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLSYTLNYADEKGKFDELVEDKD 80
           R+Q+L L++ AAARIRQLLQ +Q+ FLRL V+++GCNGLSY LNYA EKGKFDE+VE+K 
Sbjct: 2   RKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKG 61

Query: 81  VKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT 130
           VKILVDPKA+MHVIGT+MDFVDDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 62  VKILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

BLAST of CmaCh18G009490 vs. ExPASy Swiss-Prot
Match: Q07821 (Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 7.6e-28
Identity = 57/107 (53.27%), Postives = 79/107 (73.83%), Query Frame = 0

Query: 21  RRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLSYTLNYADEKGKFDELVEDKD 80
           R+ ++TL+  A   +R LL Q +   +R+  ++RGC+GL+Y L Y  E GKFDE+VE   
Sbjct: 143 RKALITLSPKAIKHLRALLAQPEPKLIRVSARNRGCSGLTYDLQYITEPGKFDEVVEQDG 202

Query: 81  VKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFM 128
           VKI++D KAL  +IG++MD++DDKL S+F+F NPNSKG CGCGESFM
Sbjct: 203 VKIVIDSKALFSIIGSEMDWIDDKLASKFVFKNPNSKGTCGCGESFM 249

BLAST of CmaCh18G009490 vs. ExPASy Swiss-Prot
Match: Q54VS1 (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=isca1 PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 9.9e-28
Identity = 57/118 (48.31%), Postives = 90/118 (76.27%), Query Frame = 0

Query: 15  KVGPAARRQILTLTEAAAARIRQLLQQRQRS---FLRLGVKSRGCNGLSYTLNYADEKGK 74
           K+G  +++ I ++T++A  R+++++  ++      LRLG+K RGC+G+SYTL++A +K K
Sbjct: 8   KLGKPSKKAIFSMTDSALKRVKEIMNDKKIDNCIGLRLGIKERGCSGMSYTLDFATQKNK 67

Query: 75  FDE-LVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 129
           FDE +V DKD+ I+VD KAL+ VIGT+MD++++ ++ EFIFINPN+   CGCGESF T
Sbjct: 68  FDETVVADKDINIIVDSKALLSVIGTEMDYIEEPIKKEFIFINPNATNTCGCGESFTT 125

BLAST of CmaCh18G009490 vs. ExPASy Swiss-Prot
Match: Q8T3X9 (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster OX=7227 GN=MagR PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.1e-26
Identity = 61/106 (57.55%), Postives = 76/106 (71.70%), Query Frame = 0

Query: 22  RQILTLTEAAAARIRQLLQQRQRSF-LRLGVKSRGCNGLSYTLNYADEKGKFDELVEDKD 81
           R  LTLT AA  RI+ LLQ +     L++GV+ RGCNGLSYTL+YA +K K DE V    
Sbjct: 23  RAALTLTPAAVLRIKTLLQDKPDMVGLKVGVRQRGCNGLSYTLDYASQKDKLDEEVVQDG 82

Query: 82  VKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESF 127
           VK+ +D KA + ++GT+MDFV+ KL SEF+F NPN KG CGCGESF
Sbjct: 83  VKVFIDKKAQLSLLGTEMDFVESKLSSEFVFNNPNIKGTCGCGESF 128

BLAST of CmaCh18G009490 vs. ExPASy TrEMBL
Match: A0A6J1HTC1 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467613 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 8.6e-67
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 0

Query: 1   MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS 60
           MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS
Sbjct: 1   MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAKAVEQ 138
           GCGESFMTTGSAKAVEQ
Sbjct: 121 GCGESFMTTGSAKAVEQ 137

BLAST of CmaCh18G009490 vs. ExPASy TrEMBL
Match: A0A6J1FZY7 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111449438 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 7.5e-63
Identity = 129/133 (96.99%), Postives = 131/133 (98.50%), Query Frame = 0

Query: 1   MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS 60
           MAASSSSF A ASQKVGPAARRQILTLTEAAAARIR+LLQQRQRSFLRLGVK+RGCNGLS
Sbjct: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRELLQQRQRSFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAK 134
           GCGESFMTTGSAK
Sbjct: 121 GCGESFMTTGSAK 133

BLAST of CmaCh18G009490 vs. ExPASy TrEMBL
Match: A0A6J1JJH4 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111484969 PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 7.1e-61
Identity = 125/134 (93.28%), Postives = 131/134 (97.76%), Query Frame = 0

Query: 1   MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS 60
           MAASSSSF A ASQKVGPAARRQILTLTEAAAARIRQLLQQRQR FLRLGVK+RGCNGLS
Sbjct: 19  MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 78

Query: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVED++VKIL++PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 79  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 138

Query: 121 GCGESFMTTGSAKA 135
           GCGESFMTTGSA+A
Sbjct: 139 GCGESFMTTGSAEA 152

BLAST of CmaCh18G009490 vs. ExPASy TrEMBL
Match: A0A6J1GZ87 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111458498 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.2e-60
Identity = 124/134 (92.54%), Postives = 132/134 (98.51%), Query Frame = 0

Query: 1   MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS 60
           MAASSSSF+A ASQKVGPAARR+ILTLTEAAAARIRQLLQQRQR FLRLGVK+RGCNGLS
Sbjct: 1   MAASSSSFLAAASQKVGPAARRKILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVED++VKIL++PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAKA 135
           GCGESFMTTGSA+A
Sbjct: 121 GCGESFMTTGSAEA 134

BLAST of CmaCh18G009490 vs. ExPASy TrEMBL
Match: A0A6J1D9T8 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111018687 PE=4 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.7e-60
Identity = 123/134 (91.79%), Postives = 130/134 (97.01%), Query Frame = 0

Query: 1   MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS 60
           MAASSSSF A AS+KVGPAARRQ+LTLTEAAA RIRQLLQQRQR FLRLGVK+RGCNGLS
Sbjct: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVED+DVKIL++PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAKA 135
           GCGESFMTTGSA+A
Sbjct: 121 GCGESFMTTGSAEA 134

BLAST of CmaCh18G009490 vs. NCBI nr
Match: XP_022968348.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima] >XP_022968350.1 iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima] >XP_022968351.1 iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima] >XP_022968352.1 iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima] >XP_022968353.1 iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 262.7 bits (670), Expect = 1.8e-66
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 0

Query: 1   MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS 60
           MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS
Sbjct: 1   MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAKAVEQ 138
           GCGESFMTTGSAKAVEQ
Sbjct: 121 GCGESFMTTGSAKAVEQ 137

BLAST of CmaCh18G009490 vs. NCBI nr
Match: XP_022945100.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata] >KAG6573887.1 Iron-sulfur assembly protein IscA-like 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012951.1 Iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 249.6 bits (636), Expect = 1.6e-62
Identity = 129/133 (96.99%), Postives = 131/133 (98.50%), Query Frame = 0

Query: 1   MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS 60
           MAASSSSF A ASQKVGPAARRQILTLTEAAAARIR+LLQQRQRSFLRLGVK+RGCNGLS
Sbjct: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRELLQQRQRSFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAK 134
           GCGESFMTTGSAK
Sbjct: 121 GCGESFMTTGSAK 133

BLAST of CmaCh18G009490 vs. NCBI nr
Match: XP_023541365.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023541366.1 iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 246.1 bits (627), Expect = 1.7e-61
Identity = 130/134 (97.01%), Postives = 131/134 (97.76%), Query Frame = 0

Query: 1   MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS 60
           MAASSSSF A ASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVK+RGCNGLS
Sbjct: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFM-TTGSAK 134
           GCGESFM TTGSAK
Sbjct: 121 GCGESFMTTTGSAK 134

BLAST of CmaCh18G009490 vs. NCBI nr
Match: XP_038891787.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 244.6 bits (623), Expect = 5.0e-61
Identity = 126/134 (94.03%), Postives = 131/134 (97.76%), Query Frame = 0

Query: 1   MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS 60
           MAASSSSF A ASQKVGPAARRQILTLTEAAAARIRQLLQQRQR FLRLGVK+RGCNGLS
Sbjct: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVED++VKIL+DPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAKA 135
           GCGESFMTTGSA+A
Sbjct: 121 GCGESFMTTGSAEA 134

BLAST of CmaCh18G009490 vs. NCBI nr
Match: XP_023539778.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 243.8 bits (621), Expect = 8.6e-61
Identity = 125/134 (93.28%), Postives = 132/134 (98.51%), Query Frame = 0

Query: 1   MAASSSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLS 60
           MAASSSSF+A ASQKVGPAARRQILTLTEAAAARIRQLLQQRQR FLRLGVK+RGCNGLS
Sbjct: 1   MAASSSSFLAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVED++VKIL++PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAKA 135
           GCGESFMTTGSA+A
Sbjct: 121 GCGESFMTTGSAEA 134

BLAST of CmaCh18G009490 vs. TAIR 10
Match: AT2G16710.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 207.2 bits (526), Expect = 8.3e-54
Identity = 100/130 (76.92%), Postives = 118/130 (90.77%), Query Frame = 0

Query: 5   SSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLSYTLN 64
           +S  +A A+ +VGPA R+Q+LTLT+ AA+R+  LLQQRQ+ FLRLGVK+RGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGE 124
           YADEKGKFDELVE+K V+ILV+PKALMHVIGTKMDFVDDKLRSEF+FINPNS+GQCGCGE
Sbjct: 63  YADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGE 122

Query: 125 SFMTTGSAKA 135
           SFMTT ++ A
Sbjct: 123 SFMTTSTSSA 132

BLAST of CmaCh18G009490 vs. TAIR 10
Match: AT2G16710.3 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 200.7 bits (509), Expect = 7.7e-52
Identity = 100/136 (73.53%), Postives = 118/136 (86.76%), Query Frame = 0

Query: 5   SSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLSYTLN 64
           +S  +A A+ +VGPA R+Q+LTLT+ AA+R+  LLQQRQ+ FLRLGVK+RGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YA------DEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKG 124
           YA      DEKGKFDELVE+K V+ILV+PKALMHVIGTKMDFVDDKLRSEF+FINPNS+G
Sbjct: 63  YAGKTSGNDEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQG 122

Query: 125 QCGCGESFMTTGSAKA 135
           QCGCGESFMTT ++ A
Sbjct: 123 QCGCGESFMTTSTSSA 138

BLAST of CmaCh18G009490 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 169.9 bits (429), Expect = 1.5e-42
Identity = 81/109 (74.31%), Postives = 98/109 (89.91%), Query Frame = 0

Query: 21  RRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLSYTLNYADEKGKFDELVEDKD 80
           R+Q+L L++ AAARIRQLLQ +Q+ FLRL V+++GCNGLSY LNYA EKGKFDE+VE+K 
Sbjct: 2   RKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKG 61

Query: 81  VKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT 130
           VKILVDPKA+MHVIGT+MDFVDDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 62  VKILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

BLAST of CmaCh18G009490 vs. TAIR 10
Match: AT2G16710.2 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 157.5 bits (397), Expect = 7.5e-39
Identity = 78/102 (76.47%), Postives = 92/102 (90.20%), Query Frame = 0

Query: 5   SSSFVATASQKVGPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLSYTLN 64
           +S  +A A+ +VGPA R+Q+LTLT+ AA+R+  LLQQRQ+ FLRLGVK+RGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YADEKGKFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLR 107
           YADEKGKFDELVE+K V+ILV+PKALMHVIGTKMDFVDDKLR
Sbjct: 63  YADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLR 104

BLAST of CmaCh18G009490 vs. TAIR 10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein )

HSP 1 Score: 69.7 bits (169), Expect = 2.1e-12
Identity = 40/123 (32.52%), Postives = 69/123 (56.10%), Query Frame = 0

Query: 9   VATASQKVGPAAR--RQILTLTEAAAARIRQLLQQRQRSF-LRLGVKSRGCNGLSYTLNY 68
           V +AS    PA    +  ++L+E A   + ++  +R     LR+GVK  GC+G+SYT+++
Sbjct: 54  VRSASVPAAPAMEGLKPAISLSENALKHLSKMRSERGEDLCLRIGVKQGGCSGMSYTMDF 113

Query: 69  ADEKG--KFDELVEDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCG 127
            +       D  +E +   I+ DPK+++ + G ++D+ D  +   F F NPN+   CGCG
Sbjct: 114 ENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYSDALIGGGFSFSNPNATQTCGCG 173

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LBM41.2e-5276.92Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana ... [more]
Q8L8C02.1e-4174.31Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana ... [more]
Q078217.6e-2853.27Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 /... [more]
Q54VS19.9e-2848.31Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Dictyostelium discoideu... [more]
Q8T3X91.1e-2657.55Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster... [more]
Match NameE-valueIdentityDescription
A0A6J1HTC18.6e-67100.00iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita maxima OX=3... [more]
A0A6J1FZY77.5e-6396.99iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita moschata OX... [more]
A0A6J1JJH47.1e-6193.28iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita maxima OX=3... [more]
A0A6J1GZ871.2e-6092.54iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita moschata OX... [more]
A0A6J1D9T82.7e-6091.79iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Momordica charantia O... [more]
Match NameE-valueIdentityDescription
XP_022968348.11.8e-66100.00iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima] >XP_0... [more]
XP_022945100.11.6e-6296.99iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata] >KA... [more]
XP_023541365.11.7e-6197.01iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita pepo subsp. p... [more]
XP_038891787.15.0e-6194.03iron-sulfur assembly protein IscA-like 1, mitochondrial isoform X1 [Benincasa hi... [more]
XP_023539778.18.6e-6193.28iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita pepo subsp. p... [more]
Match NameE-valueIdentityDescription
AT2G16710.18.3e-5476.92Iron-sulphur cluster biosynthesis family protein [more]
AT2G16710.37.7e-5273.53Iron-sulphur cluster biosynthesis family protein [more]
AT2G36260.11.5e-4274.31Iron-sulphur cluster biosynthesis family protein [more]
AT2G16710.27.5e-3976.47Iron-sulphur cluster biosynthesis family protein [more]
AT1G10500.12.1e-1232.52chloroplast-localized ISCA-like protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000361FeS cluster biogenesisPFAMPF01521Fe-S_biosyncoord: 25..124
e-value: 2.4E-21
score: 76.0
IPR035903HesB-like domain superfamilyGENE3D2.60.300.12coord: 24..130
e-value: 5.2E-35
score: 121.9
IPR035903HesB-like domain superfamilySUPERFAMILY89360HesB-like domaincoord: 24..117
IPR016092FeS cluster insertion proteinTIGRFAMTIGR00049TIGR00049coord: 25..127
e-value: 3.6E-35
score: 118.4
NoneNo IPR availablePANTHERPTHR10072IRON-SULFUR CLUSTER ASSEMBLY PROTEINcoord: 1..135
NoneNo IPR availablePANTHERPTHR10072:SF60IRON-SULFUR ASSEMBLY PROTEIN ISCA-LIKE 3, MITOCHONDRIALcoord: 1..135
IPR017870FeS cluster insertion, C-terminal, conserved sitePROSITEPS01152HESBcoord: 109..126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G009490.1CmaCh18G009490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0097428 protein maturation by iron-sulfur cluster transfer
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0051536 iron-sulfur cluster binding