CmaCh18G008660 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh18G008660
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRING-type E3 ubiquitin transferase
LocationCma_Chr18: 7539215 .. 7543633 (+)
RNA-Seq ExpressionCmaCh18G008660
SyntenyCmaCh18G008660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTTCGTTTGGCGATTGAAAAAGTTCAAAAAAGCTCTGAAAATTTCCCTTTCGATCATAATAAAATCAATTTTCAGAATTTCGAGGACACTGAAGCCGGCCTGCTTGTTATGGGCAAGGAGAGAAGGGCCCGCACGTCTTAATTTCTCTCTTTTTCTTTCACTTTTTGTTCTTTCTCGAGAAATTTCCCTCTAATCCTCTAATCTCTTCTGTTCTTCATACGCTCCTCTTTCCTCTCTCTCCCTATCACCCTTTAATTTGAATTTTCGCGGCTCCCAAACGTGTAATATCTCTGTGTATTATCTCTTTTTATGCTGTTTCTTGTCGTATCACACCCCGAGTCTGATTATCTTTTGAGGTATTTCTTGTTTTTGTTTGTTCTTCTTTGATTTCTTATGGGGTTTTTGAGTCGTTATGATTTGGGGTTTGCGTTATTTCTGGTTTTATTATTGTCTTGTGTTGTTTGAGGTCTTTGATTTTGGATGTTCTAATCTGCATTCCGTTTGTTGGTTGTCTCATTGTGACTCTGGATATTTGTTGTTCATATTTTTTTTGTGTTGTTCTTCTTTTGGAGCTCTGGATTTAGAAAATTTGTTGATATCATAACATTAAAGTTTTGGGTAATGATAGTTGAATTGGTTCCTTTGGGTTTTATTTTGTGTTTTTCATTCATTTATTCCATCGGATTAACGGTTCCATTCTCATGATTCTGAACTTGGAGGTATTCCATAAATTGCTCGAGCTTCTTGTTCTTGTTCTTTTTCTCCGTTGGATTTGGGTGTTCTGGTTTCTTGTCTAATGGCTTGAGATGCTTTCTTTTTCTTGTAGTGAAGCATCTTTGTGGTTAGTAATTCATAGTTTTTATGTTTTGGTTTGCAATTTGCTAAGTTTGGGTGAAGGTAATGTTGAGGATTGTTGGGAGGGAGTCCTACGTTGGCTAATCTAGGGAATGATCGTGGGTTTATAAGTGAGGAATACTTCTCCATTGGTATGAGGCCTTTTGGGGAAGCCCGAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGATCCGTGATTCCTAATAGGGTATTAGAGTCATGCCCTTAGCTTAGTCATGTTAATAGAATTCTCAAATGTTGAACAAAGAAAGTTGTGAGTCTCGAAGGTGTAGTAAAAAATTGACTCAAGTGTCGAACAAATGGTGTACTTTGTTCGAGGACTCCATAGGCCTCGGGGAAGGCTCTATAGTGTACTTTGTTTGAGTAGAGGATTGTTGAGGATTGTTGGGAGGGAGTCTCACATTCACTGATTTAGGTAATATTCGTGAGTTTATAAGTACGTAATATATCTCCATTGGTATGAGGCCTTTTGGGAAGCTTGAAGCAAAGCCACGAGAGTTTATACTCAACGTGGACAATATCATACCATTGGGGAGATCCGTGATTCCTAACATGGTATCAGAGTAATGCCCTTAACTTAGCCATGTCAATAGAATCCTCAAATGTGGAACAAAGAAAGTTGTGAGTCTCGAAGGTATAGTCAAAAGTGACTCAAGTGTCGAACAAAGAGTGTTCTTTGTTCGAGTACTCCAGAAAAGAAGTCGAGCCTCGATTAAGGGGAGACTGTTTGAGGGCTCCATAGACCTTGTGGGAGGCTCTATAATGTTCTTTGTTCGAGGAGAGGATTATTGAGGGTTATTGGGAGGGAGTTCCACATTGGCTAATTTAGGGAATGATCATGGGTTTATAAGTAAGTAATACATCTCCATGGTTACGAGGTCTTTTGGGGAAGCCCAAAGCAAAGCCAATGAGAACTTATACTCAAAGTGGACTATATCCATTGTGGAGAGTCGTTATTCCTAAAAAGTAACCCTTCACTCTTTATCGATGGGTTTTGGCTTCTGATAGCCCCTAGAGCTTTACTAGAACACGTCCTTATCTGGTGAAGCACTCTTCTCAAGAAGCTTAGTTGCTGTGAAGATGTTCTTATACTTGCAAGTTCTTGTTCATACTTTTCTTAACTCATGTTTATGGATGTAGGCATTCTATTGATTAAAATGTCGTTTGCAAATCCTTATTTTGAAGACGTTCGGACGAAACCGGAGGTAATCGATCCTCCCCAAGATGAGGACATGATGGATGTTAGTGAAAGTGTTAGTGATCCTACTCGAAATGGAGGAAAGCCTAATGTAGCTGTCTCCAGCAGCGTTCGTGAGCTGTTGGAGTGTCCTGTTTGCTTGAATGTTATGTACCCTCCCATTCATCATGTCTGTTGCCATCTTTTCAAAATCTTTTGAACGAAATTGCTTACTTTTTTTGAATTCCTTTAATCTAATTTGTCGAGACGACATTGGCCTTTTTCTTAAAGGCGTCTAAGGAAGGTACCATATAGGTGAACGTATTCATTAGTTTAAACGAACAACTAGAAGCCATACCGTACCTTGGAAGATAGATGATTTTGTGATAAGGAATCACTACAAAAGAAGTAAGATTTGGATTATTTTGTTATCAAATATCACAAGCAAAATTGATTGTGTCAAGCTTGAATGATTCTAAACATGCAATCTAAACTACGTAGAGTTGAAAAAAAAAAAAAAAGAACTTAGCCCTCGGATAAAGAAAAGGTAAGAACGGTGAGATCTCTAGTGAGGACACTGGGCCCCGAAGTTGGGTTGATTGCAAGATCCCACATCGGTTGGAGAGGGTAACAAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTAACAGACACGTTTTAAAAACTTTGAGGGGAAAGGGAAAGCCCAAAGGGACAATATTTGCTAGCGGTGTGCTTGGGCTATTACAAAAACACTTAGCTCTGATGATGGAAACTAAAATTCGGATTACCTTGTTGATCAAATACCTCAAGGTAAGAACACTTTATTTGAGATACGAATCACTCCACAAGCAAGATCGATCATATCAAGCTTGAATGATTCTAAACATGCAATCTAAATTACATAGAATTGCAAAGAAACTTAGTCATTGGCTAAAAGAAAACACAAATGCTCTTTTTACTCGTCCCTTTCACAAAGACAACATATACCGCTATATATAGCTTCAAAATAAAAACCTTAACCTTAGGCATTTCAAGAGCCGTAACTTTCATAGTATAACAGGTTAGATGTATGATCGGGATGATATCTACCGTGTTAATACCACCTAAAATCAACGTTGAAATCTTACTTTCTTAATATAGTTGTGCTTTGCATGTATGTAATCTGCTACTTGCTCAGAACCTTCATTTTGGCATTTGAGTTGTGCTCCCTTTTAACCTGAGTTGATTGTTCGCCTTGGTTCATATTGATTAACGTTCTGTCATTGCAGTGTTCGAATGGTCACACATTGTGTTCGGGTTGCAAGCCTAGGGTGCACAACCGGTGCCCGACCTGCAGGCATGAACTTGGCAATATCAGATGTCTTGCATTAGAGAAAGTGGCTGCATCTTTGGAACTTCCATGTACATATCAAACCTTTGGGTGCGCGGGCATATACCCGTACTATAGCAAGCTGAAACATGAATCTCAATGTGCCTATCGACCCTATAACTGTCCGTATGCGGGGTCGGAATGCATGGTTGTTGGTGATATTCCATTTTTGGTAGCACACCTAAAGGATGATCACAAAGTCGACATGCATAATGGGAGCACTTTCAATCATCGATACGTCAAGTCAAACCCGCAGGAGGTCGAAAATGCTACCTGGATGCTAACGGTACTATTCTACTTCATATTATTAGTCATTTTTGGGTTAAACATGTATTCAAATGAAGCTTTCGTTATCAAGAATGAGCGTATTAGCTAGAAGAGACTAACGAATCGAGTTCATAGGTTGTAAATATTGAGATGTCTAATACAAAACTGATACCGTTTTTTCGGTTGACTTGTAGGTTTTCAGCTGTTTTGGTCATTATTTTTGTTTACATTTCGAAGCTTTCCAACTCGGAATGGCACCTGTCTATATAGCCTTCTTGCGCTTCATGGGTGACGATAACGAGGTGAAGAACTACAGTTATAGCCTTGAGGTTGGTGGAAATGGGAGGAAGATGGTTTGGCAAGGAGTGCCACGGAGTATTCGAGACAGTCATCGGAAGGTGCGAGACAGCTTTGACGGGCTTATTATCCAACGAAACATGGCTCTGTTCTTCTCGGGCGGAGATCGGAAGGAATTGAAGCTGAGGGTAACTGGTAGGATTTGGAAGGAGCAGTGAAAGTGTAGGTATGTTTAGGTGTCCTTAGAAAGTAAAGGAGGCTAAATAGAGAGAATGATAATATAGACGTCCAACCATGTAATGTAATATAGTTGTTCAAATACATCAACTGGAAAGCCTGTGTTCTTTACTCGTTGTAAGAGAATTTCTACTTACGTTGCACTTTTAGGTCCATCCCGAGAAGAA

mRNA sequence

TATTTCGTTTGGCGATTGAAAAAGTTCAAAAAAGCTCTGAAAATTTCCCTTTCGATCATAATAAAATCAATTTTCAGAATTTCGAGGACACTGAAGCCGGCCTGCTTGTTATGGGCAAGGAGAGAAGGGCCCGCACGTCTTAATTTCTCTCTTTTTCTTTCACTTTTTGTTCTTTCTCGAGAAATTTCCCTCTAATCCTCTAATCTCTTCTGTTCTTCATACGCTCCTCTTTCCTCTCTCTCCCTATCACCCTTTAATTTGAATTTTCGCGGCTCCCAAACGTGTAATATCTCTGTGTATTATCTCTTTTTATGCTGTTTCTTGTCGTATCACACCCCGAGTCTGATTATCTTTTGAGGCATTCTATTGATTAAAATGTCGTTTGCAAATCCTTATTTTGAAGACGTTCGGACGAAACCGGAGGTAATCGATCCTCCCCAAGATGAGGACATGATGGATGTTAGTGAAAGTGTTAGTGATCCTACTCGAAATGGAGGAAAGCCTAATGTAGCTGTCTCCAGCAGCGTTCGTGAGCTGTTGGAGTGTCCTGTTTGCTTGAATGTTATGTACCCTCCCATTCATCATTGTTCGAATGGTCACACATTGTGTTCGGGTTGCAAGCCTAGGGTGCACAACCGGTGCCCGACCTGCAGGCATGAACTTGGCAATATCAGATGTCTTGCATTAGAGAAAGTGGCTGCATCTTTGGAACTTCCATGTACATATCAAACCTTTGGGTGCGCGGGCATATACCCGTACTATAGCAAGCTGAAACATGAATCTCAATGTGCCTATCGACCCTATAACTGTCCGTATGCGGGGTCGGAATGCATGGTTGTTGGTGATATTCCATTTTTGGTAGCACACCTAAAGGATGATCACAAAGTCGACATGCATAATGGGAGCACTTTCAATCATCGATACGTCAAGTCAAACCCGCAGGAGGTCGAAAATGCTACCTGGATGCTAACGGTTTTCAGCTGTTTTGGTCATTATTTTTGTTTACATTTCGAAGCTTTCCAACTCGGAATGGCACCTGTCTATATAGCCTTCTTGCGCTTCATGGGTGACGATAACGAGGTGAAGAACTACAGTTATAGCCTTGAGGTTGGTGGAAATGGGAGGAAGATGGTTTGGCAAGGAGTGCCACGGAGTATTCGAGACAGTCATCGGAAGGTGCGAGACAGCTTTGACGGGCTTATTATCCAACGAAACATGGCTCTGTTCTTCTCGGGCGGAGATCGGAAGGAATTGAAGCTGAGGGTAACTGGTAGGATTTGGAAGGAGCAGTGAAAGTGTAGGTATGTTTAGGTGTCCTTAGAAAGTAAAGGAGGCTAAATAGAGAGAATGATAATATAGACGTCCAACCATGTAATGTAATATAGTTGTTCAAATACATCAACTGGAAAGCCTGTGTTCTTTACTCGTTGTAAGAGAATTTCTACTTACGTTGCACTTTTAGGTCCATCCCGAGAAGAA

Coding sequence (CDS)

ATGTCGTTTGCAAATCCTTATTTTGAAGACGTTCGGACGAAACCGGAGGTAATCGATCCTCCCCAAGATGAGGACATGATGGATGTTAGTGAAAGTGTTAGTGATCCTACTCGAAATGGAGGAAAGCCTAATGTAGCTGTCTCCAGCAGCGTTCGTGAGCTGTTGGAGTGTCCTGTTTGCTTGAATGTTATGTACCCTCCCATTCATCATTGTTCGAATGGTCACACATTGTGTTCGGGTTGCAAGCCTAGGGTGCACAACCGGTGCCCGACCTGCAGGCATGAACTTGGCAATATCAGATGTCTTGCATTAGAGAAAGTGGCTGCATCTTTGGAACTTCCATGTACATATCAAACCTTTGGGTGCGCGGGCATATACCCGTACTATAGCAAGCTGAAACATGAATCTCAATGTGCCTATCGACCCTATAACTGTCCGTATGCGGGGTCGGAATGCATGGTTGTTGGTGATATTCCATTTTTGGTAGCACACCTAAAGGATGATCACAAAGTCGACATGCATAATGGGAGCACTTTCAATCATCGATACGTCAAGTCAAACCCGCAGGAGGTCGAAAATGCTACCTGGATGCTAACGGTTTTCAGCTGTTTTGGTCATTATTTTTGTTTACATTTCGAAGCTTTCCAACTCGGAATGGCACCTGTCTATATAGCCTTCTTGCGCTTCATGGGTGACGATAACGAGGTGAAGAACTACAGTTATAGCCTTGAGGTTGGTGGAAATGGGAGGAAGATGGTTTGGCAAGGAGTGCCACGGAGTATTCGAGACAGTCATCGGAAGGTGCGAGACAGCTTTGACGGGCTTATTATCCAACGAAACATGGCTCTGTTCTTCTCGGGCGGAGATCGGAAGGAATTGAAGCTGAGGGTAACTGGTAGGATTTGGAAGGAGCAGTGA

Protein sequence

MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVCLNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ
Homology
BLAST of CmaCh18G008660 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 1.8e-154
Identity = 253/290 (87.24%), Postives = 264/290 (91.03%), Query Frame = 0

Query: 16  EVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVCLNVMYPPIHHCSNGH 75
           EVIDPP+ E ++DV+E   DP  +  KPNV VSSSVRELLECPVCL+ MYPPIH CSNGH
Sbjct: 13  EVIDPPKTE-VLDVTELAGDPVPHSPKPNVVVSSSVRELLECPVCLSAMYPPIHQCSNGH 72

Query: 76  TLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHE 135
           TLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPC YQ FGC GIYPYY KLKHE
Sbjct: 73  TLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQNFGCVGIYPYYCKLKHE 132

Query: 136 SQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENAT 195
           SQC YRPY+CPYAGSEC V GDIP+LV HLKDDHKVDMHNG TFNHRYVKSNP EVENAT
Sbjct: 133 SQCQYRPYSCPYAGSECTVAGDIPYLVNHLKDDHKVDMHNGCTFNHRYVKSNPHEVENAT 192

Query: 196 WMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYSYSLEVGGNGRKMVWQ 255
           WMLTVFSCFG YFCLHFEAFQLGMAPVYIAFLRFMGDD E KNYSYSLEVGG GRKM+WQ
Sbjct: 193 WMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDLEAKNYSYSLEVGGTGRKMIWQ 252

Query: 256 GVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
           GVPRSIRDSHRKVRDS+DGLIIQRNMALFFSGG+RKELKLRVTGRIWKEQ
Sbjct: 253 GVPRSIRDSHRKVRDSYDGLIIQRNMALFFSGGERKELKLRVTGRIWKEQ 301

BLAST of CmaCh18G008660 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 477.2 bits (1227), Expect = 1.3e-133
Identity = 221/289 (76.47%), Postives = 242/289 (83.74%), Query Frame = 0

Query: 18  IDPPQDEDMMDVSESVSDPTRNGGKPNV-AVSSSVRELLECPVCLNVMYPPIHHCSNGHT 77
           ID   D+D +      S     GG   V + ++SV ELLECPVC N MYPPIH C NGHT
Sbjct: 6   IDSVIDDDEIHQKHQFSSTKSQGGATVVISPATSVYELLECPVCTNSMYPPIHQCHNGHT 65

Query: 78  LCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHES 137
           LCS CK RVHNRCPTCR ELG+IRCLALEKVA SLELPC Y   GC GI+PYYSKLKHES
Sbjct: 66  LCSTCKSRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYNLGCLGIFPYYSKLKHES 125

Query: 138 QCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATW 197
           QC +RPY+CPYAGSEC  VGDI FLVAHL+DDHKVDMH G TFNHRYVKSNP+EVENATW
Sbjct: 126 QCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATW 185

Query: 198 MLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYSYSLEVGGNGRKMVWQG 257
           MLTVF CFG YFCLHFEAFQLGMAPVY+AFLRFMGD+++ +NY+YSLEVGG+GRK  W+G
Sbjct: 186 MLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEG 245

Query: 258 VPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
            PRS+RDSHRKVRDS DGLIIQRNMALFFSGGD+KELKLRVTGRIWKEQ
Sbjct: 246 TPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDKKELKLRVTGRIWKEQ 294

BLAST of CmaCh18G008660 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 1.5e-132
Identity = 212/258 (82.17%), Postives = 232/258 (89.92%), Query Frame = 0

Query: 48  SSSVRELLECPVCLNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKV 107
           ++SV ELLECPVC N MYPPIH C NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKV
Sbjct: 54  TTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKV 113

Query: 108 AASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKD 167
           A SLELPC + + GC  I+PYYSKLKHE+ C +RPY+CPYAGSEC V GDIPFLVAHL+D
Sbjct: 114 AESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRD 173

Query: 168 DHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFL 227
           DHKVDMH+G TFNHRYVKSNP+EVENATWMLTVF CFG YFCLHFEAFQLGMAPVY+AFL
Sbjct: 174 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 233

Query: 228 RFMGDDNEVKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSG 287
           RFMGD+ E +NY+YSLEVGG GRK++W+G PRS+RDSHRKVRDS DGLIIQRNMALFFSG
Sbjct: 234 RFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 293

Query: 288 GDRKELKLRVTGRIWKEQ 306
           GDRKELKLRVTGRIWKEQ
Sbjct: 294 GDRKELKLRVTGRIWKEQ 311

BLAST of CmaCh18G008660 vs. ExPASy Swiss-Prot
Match: Q9STN8 (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=1 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.1e-130
Identity = 213/265 (80.38%), Postives = 232/265 (87.55%), Query Frame = 0

Query: 41  GKPNVAVSSSVRELLECPVCLNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIR 100
           G    A ++SV ELLECPVC   MYPPIH C NGHTLCS CK RVHNRCPTCR ELG+IR
Sbjct: 48  GPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIR 107

Query: 101 CLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPF 160
           CLALEKVA SLELPC +   GC  I+PYYSKLKHES C +RPY+CPYAGSEC +VGDIPF
Sbjct: 108 CLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPF 167

Query: 161 LVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMA 220
           LVAHL+DDHKVDMH GSTFNHRYVKSNP+EVENATWMLTVF CFG YFCLHFEAFQLGM 
Sbjct: 168 LVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMG 227

Query: 221 PVYIAFLRFMGDDNEVKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRN 280
           PVY+AFLRFMGD+ + ++YSYSLEVGG+GRK+ W+G PRSIRDSHRKVRDS DGLIIQRN
Sbjct: 228 PVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRN 287

Query: 281 MALFFSGGDRKELKLRVTGRIWKEQ 306
           MALFFSGGDRKELKLRVTG+IWKEQ
Sbjct: 288 MALFFSGGDRKELKLRVTGKIWKEQ 312

BLAST of CmaCh18G008660 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 2.6e-129
Identity = 211/274 (77.01%), Postives = 232/274 (84.67%), Query Frame = 0

Query: 36  PTRNG----GKPNVAVSSSVRELLECPVCLNVMYPPIHHCSNGHTLCSGCKPRVHNRCPT 95
           PT+ G    GK  +  ++ V ELLECPVC N+MYPPIH C NGHTLCS CK RV N CPT
Sbjct: 35  PTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPT 94

Query: 96  CRHELGNIRCLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSE 155
           CR+ELGNIRCLALEKVA SLE+PC YQ  GC  I+PYYSKLKHE  C +RPY CPYAGSE
Sbjct: 95  CRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSE 154

Query: 156 CMVVGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLH 215
           C V GDIP LV HLKDDHKVDMH+G TFNHRYVKSNP EVENATWMLTVF+CFG  FCLH
Sbjct: 155 CSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLH 214

Query: 216 FEAFQLGMAPVYIAFLRFMGDDNEVKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDS 275
           FEAFQLGMAPVY+AFLRFMGD+NE K +SYSLEVG +GRK+ WQG+PRSIRDSHRKVRDS
Sbjct: 215 FEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDS 274

Query: 276 FDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
            DGLII RN+AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 275 QDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of CmaCh18G008660 vs. ExPASy TrEMBL
Match: A0A6J1HQW6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466544 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 1.7e-184
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 12  MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 71

Query: 61  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 72  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 131

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 132 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 191

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS
Sbjct: 192 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 251

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 252 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 311

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 312 IWKEQ 316

BLAST of CmaCh18G008660 vs. ExPASy TrEMBL
Match: A0A6J1HPK3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466544 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 1.7e-184
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 1   MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 61  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of CmaCh18G008660 vs. ExPASy TrEMBL
Match: A0A6J1G1E2 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449792 PE=3 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 4.4e-180
Identity = 298/305 (97.70%), Postives = 300/305 (98.36%), Query Frame = 0

Query: 1   MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MS ANPYF+DVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LN MYPPIH CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSEC VVGDIPFLVAHLKDDHKVDMH+GSTFN
Sbjct: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNE KNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of CmaCh18G008660 vs. ExPASy TrEMBL
Match: A0A6J1G1D3 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449792 PE=3 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 4.4e-180
Identity = 298/305 (97.70%), Postives = 300/305 (98.36%), Query Frame = 0

Query: 1   MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MS ANPYF+DVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 12  MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 71

Query: 61  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LN MYPPIH CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 72  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 131

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSEC VVGDIPFLVAHLKDDHKVDMH+GSTFN
Sbjct: 132 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHSGSTFN 191

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNE KNYS
Sbjct: 192 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 251

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 252 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 311

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 312 IWKEQ 316

BLAST of CmaCh18G008660 vs. ExPASy TrEMBL
Match: A0A6J1GX87 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111458334 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.1e-175
Identity = 291/305 (95.41%), Postives = 293/305 (96.07%), Query Frame = 0

Query: 1   MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MS ANPYF+DVRTKPEVIDPPQD+DMMDVSESVSDP RNGGKPNVAVSSSVRELLECPVC
Sbjct: 1   MSSANPYFDDVRTKPEVIDPPQDDDMMDVSESVSDPARNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LN MYPPIH CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPC Y TF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYLTF 120

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GC GIYPYYSKLKHESQC YRPYNCPYAGSEC VVGDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 121 GCVGIYPYYSKLKHESQCVYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFG YFCLHFEAFQLGMAPVYIAFLRFMGDDNE KNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRK VWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKTVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of CmaCh18G008660 vs. NCBI nr
Match: XP_022967016.1 (E3 ubiquitin-protein ligase DIS1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 654.8 bits (1688), Expect = 3.6e-184
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 12  MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 71

Query: 61  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 72  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 131

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 132 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 191

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS
Sbjct: 192 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 251

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 252 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 311

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 312 IWKEQ 316

BLAST of CmaCh18G008660 vs. NCBI nr
Match: XP_022967017.1 (E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 654.8 bits (1688), Expect = 3.6e-184
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 1   MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 61  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of CmaCh18G008660 vs. NCBI nr
Match: XP_023541837.1 (E3 ubiquitin-protein ligase DIS1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 642.1 bits (1655), Expect = 2.4e-180
Identity = 299/305 (98.03%), Postives = 300/305 (98.36%), Query Frame = 0

Query: 1   MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MS ANPYF+DVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 12  MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 71

Query: 61  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LN MYPPIH CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 72  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 131

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSEC VVGDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 132 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 191

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNE KNYS
Sbjct: 192 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 251

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 252 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 311

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 312 IWKEQ 316

BLAST of CmaCh18G008660 vs. NCBI nr
Match: XP_023541838.1 (E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 642.1 bits (1655), Expect = 2.4e-180
Identity = 299/305 (98.03%), Postives = 300/305 (98.36%), Query Frame = 0

Query: 1   MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MS ANPYF+DVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LN MYPPIH CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSEC VVGDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNE KNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of CmaCh18G008660 vs. NCBI nr
Match: KAG7012878.1 (E3 ubiquitin-protein ligase SINAT5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 640.6 bits (1651), Expect = 7.0e-180
Identity = 298/305 (97.70%), Postives = 300/305 (98.36%), Query Frame = 0

Query: 1   MSFANPYFEDVRTKPEVIDPPQDEDMMDVSESVSDPTRNGGKPNVAVSSSVRELLECPVC 60
           MS ANPYF+DVRTKPEVIDPPQDEDMMDVS+SVSDPTRNGGKPNVAVSSSVRELLECPVC
Sbjct: 1   MSSANPYFDDVRTKPEVIDPPQDEDMMDVSKSVSDPTRNGGKPNVAVSSSVRELLECPVC 60

Query: 61  LNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120
           LN MYPPIH CSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCTYQTF 120

Query: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKDDHKVDMHNGSTFN 180
           GCAGIYPYYSKLKHESQCAYRPYNCPYAGSEC VVGDIPFLVAHLKDDHKVDMHNGSTFN
Sbjct: 121 GCAGIYPYYSKLKHESQCAYRPYNCPYAGSECTVVGDIPFLVAHLKDDHKVDMHNGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNE KNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of CmaCh18G008660 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 473.8 bits (1218), Expect = 1.1e-133
Identity = 212/258 (82.17%), Postives = 232/258 (89.92%), Query Frame = 0

Query: 48  SSSVRELLECPVCLNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKV 107
           ++SV ELLECPVC N MYPPIH C NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKV
Sbjct: 54  TTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKV 113

Query: 108 AASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFLVAHLKD 167
           A SLELPC + + GC  I+PYYSKLKHE+ C +RPY+CPYAGSEC V GDIPFLVAHL+D
Sbjct: 114 AESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRD 173

Query: 168 DHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAPVYIAFL 227
           DHKVDMH+G TFNHRYVKSNP+EVENATWMLTVF CFG YFCLHFEAFQLGMAPVY+AFL
Sbjct: 174 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 233

Query: 228 RFMGDDNEVKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSG 287
           RFMGD+ E +NY+YSLEVGG GRK++W+G PRS+RDSHRKVRDS DGLIIQRNMALFFSG
Sbjct: 234 RFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 293

Query: 288 GDRKELKLRVTGRIWKEQ 306
           GDRKELKLRVTGRIWKEQ
Sbjct: 294 GDRKELKLRVTGRIWKEQ 311

BLAST of CmaCh18G008660 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 467.6 bits (1202), Expect = 7.6e-132
Identity = 213/265 (80.38%), Postives = 232/265 (87.55%), Query Frame = 0

Query: 41  GKPNVAVSSSVRELLECPVCLNVMYPPIHHCSNGHTLCSGCKPRVHNRCPTCRHELGNIR 100
           G    A ++SV ELLECPVC   MYPPIH C NGHTLCS CK RVHNRCPTCR ELG+IR
Sbjct: 48  GPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIR 107

Query: 101 CLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPF 160
           CLALEKVA SLELPC +   GC  I+PYYSKLKHES C +RPY+CPYAGSEC +VGDIPF
Sbjct: 108 CLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPF 167

Query: 161 LVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMA 220
           LVAHL+DDHKVDMH GSTFNHRYVKSNP+EVENATWMLTVF CFG YFCLHFEAFQLGM 
Sbjct: 168 LVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMG 227

Query: 221 PVYIAFLRFMGDDNEVKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRN 280
           PVY+AFLRFMGD+ + ++YSYSLEVGG+GRK+ W+G PRSIRDSHRKVRDS DGLIIQRN
Sbjct: 228 PVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRN 287

Query: 281 MALFFSGGDRKELKLRVTGRIWKEQ 306
           MALFFSGGDRKELKLRVTG+IWKEQ
Sbjct: 288 MALFFSGGDRKELKLRVTGKIWKEQ 312

BLAST of CmaCh18G008660 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 463.0 bits (1190), Expect = 1.9e-130
Identity = 211/274 (77.01%), Postives = 232/274 (84.67%), Query Frame = 0

Query: 36  PTRNG----GKPNVAVSSSVRELLECPVCLNVMYPPIHHCSNGHTLCSGCKPRVHNRCPT 95
           PT+ G    GK  +  ++ V ELLECPVC N+MYPPIH C NGHTLCS CK RV N CPT
Sbjct: 35  PTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPT 94

Query: 96  CRHELGNIRCLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSE 155
           CR+ELGNIRCLALEKVA SLE+PC YQ  GC  I+PYYSKLKHE  C +RPY CPYAGSE
Sbjct: 95  CRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSE 154

Query: 156 CMVVGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLH 215
           C V GDIP LV HLKDDHKVDMH+G TFNHRYVKSNP EVENATWMLTVF+CFG  FCLH
Sbjct: 155 CSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLH 214

Query: 216 FEAFQLGMAPVYIAFLRFMGDDNEVKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDS 275
           FEAFQLGMAPVY+AFLRFMGD+NE K +SYSLEVG +GRK+ WQG+PRSIRDSHRKVRDS
Sbjct: 215 FEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDS 274

Query: 276 FDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
            DGLII RN+AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 275 QDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of CmaCh18G008660 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 453.4 bits (1165), Expect = 1.5e-127
Identity = 208/277 (75.09%), Postives = 231/277 (83.39%), Query Frame = 0

Query: 31  ESVSDPTRNGGKP--NVAVSSSVRELLECPVCLNVMYPPIHHCSNGHTLCSGCKPRVHNR 90
           E+ S PT++G         S+ V ELLECPVC N+MYPPIH C NGHTLCS CK RV N 
Sbjct: 29  EANSKPTKSGSGSIGKFHSSNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNT 88

Query: 91  CPTCRHELGNIRCLALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYA 150
           CPTCR+ELGNIRCLALEKVA SLE+PC YQ  GC  I+PYYSKLKHE  C +R Y+CPYA
Sbjct: 89  CPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYA 148

Query: 151 GSECMVVGDIPFLVAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYF 210
           GSEC V GDIP LV HLKDDHKVDMH+G TFNHRYVKSNP EVENATWMLTVF+CFG  F
Sbjct: 149 GSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQF 208

Query: 211 CLHFEAFQLGMAPVYIAFLRFMGDDNEVKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKV 270
           CLHFEAFQLGMAPVY+AFLRFMGD+NE K +SYSLEVG + RK+ WQG+PRSIRDSHRKV
Sbjct: 209 CLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKV 268

Query: 271 RDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
           RDS DGLII RN+AL+FSG D++ELKLRVTGRIWKE+
Sbjct: 269 RDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWKEE 305

BLAST of CmaCh18G008660 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 376.3 bits (965), Expect = 2.3e-104
Identity = 169/204 (82.84%), Postives = 185/204 (90.69%), Query Frame = 0

Query: 102 LALEKVAASLELPCTYQTFGCAGIYPYYSKLKHESQCAYRPYNCPYAGSECMVVGDIPFL 161
           +ALEKVA SLELPC Y   GC GI+PYYSKLKHESQC +RPY+CPYAGSEC  VGDI FL
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 162 VAHLKDDHKVDMHNGSTFNHRYVKSNPQEVENATWMLTVFSCFGHYFCLHFEAFQLGMAP 221
           VAHL+DDHKVDMH G TFNHRYVKSNP+EVENATWMLTVF CFG YFCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 222 VYIAFLRFMGDDNEVKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNM 281
           VY+AFLRFMGD+++ +NY+YSLEVGG+GRK  W+G PRS+RDSHRKVRDS DGLIIQRNM
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 282 ALFFSGGDRKELKLRVTGRIWKEQ 306
           ALFFSGGD+KELKLRVTGRIWKEQ
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQ 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q10L911.8e-15487.24E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS... [more]
Q8S3N11.3e-13376.47E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=... [more]
Q84JL31.5e-13282.17E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=... [more]
Q9STN81.1e-13080.38E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=... [more]
Q9M2P42.6e-12977.01E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1HQW61.7e-184100.00RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466544 P... [more]
A0A6J1HPK31.7e-184100.00RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466544 P... [more]
A0A6J1G1E24.4e-18097.70RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449792... [more]
A0A6J1G1D34.4e-18097.70RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449792... [more]
A0A6J1GX871.1e-17595.41RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111458334... [more]
Match NameE-valueIdentityDescription
XP_022967016.13.6e-184100.00E3 ubiquitin-protein ligase DIS1-like isoform X1 [Cucurbita maxima][more]
XP_022967017.13.6e-184100.00E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita maxima][more]
XP_023541837.12.4e-18098.03E3 ubiquitin-protein ligase DIS1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023541838.12.4e-18098.03E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita pepo subsp. pepo][more]
KAG7012878.17.0e-18097.70E3 ubiquitin-protein ligase SINAT5 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT3G61790.11.1e-13382.17Protein with RING/U-box and TRAF-like domains [more]
AT4G27880.17.6e-13280.38Protein with RING/U-box and TRAF-like domains [more]
AT3G58040.11.9e-13077.01seven in absentia of Arabidopsis 2 [more]
AT2G41980.11.5e-12775.09Protein with RING/U-box and TRAF-like domains [more]
AT5G53360.12.3e-10482.84TRAF-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 18..109
e-value: 3.2E-9
score: 38.4
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 110..165
e-value: 2.5E-15
score: 58.4
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 166..298
e-value: 3.0E-38
score: 132.6
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 99..298
e-value: 6.9E-79
score: 264.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..44
NoneNo IPR availablePANTHERPTHR10315:SF77E3 UBIQUITIN-PROTEIN LIGASE DIS1coord: 14..305
NoneNo IPR availablePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 14..305
NoneNo IPR availableCDDcd16571RING-HC_SIAHscoord: 55..93
e-value: 1.0894E-13
score: 62.3022
NoneNo IPR availableCDDcd03829Sinacoord: 171..299
e-value: 1.11006E-63
score: 195.298
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 110..302
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 46..99
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 110..170
score: 13.750349
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 57..93
score: 9.214112

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G008660.1CmaCh18G008660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding