CmaCh18G008060 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh18G008060
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionU4/U6 small nuclear ribonucleoprotein Prp31 homolog
LocationCma_Chr18: 7062094 .. 7065635 (-)
RNA-Seq ExpressionCmaCh18G008060
SyntenyCmaCh18G008060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCATCGGCGAACGGCGGTCGTAAAATTCAATTGTTATCTCTTCCTCTGTTTCTGACTATTTCCGTCGATCACAAGCCATTGCCAGTTTCTCCTCTTTCTTTCGTCGGCGTTCTTTCTGATCGTTCTGAGTGTGAATTTTATCTCGTCGGTTACCTTGCTGTAACATTTGGAGAGTATATTTCATCCGAGGTTTGGGATTGCCTTGTGTGTAAAAGCATCAGAGTGTGGTTTTATCAGTTTAGGTTTATATTTGTATGTTCCTAGCTCTGGATATTGAAACTATAAAATTCAGCTATTCTTCAACTTGGTGCTGAATTGATTACGACCAAGACAACCAGGGAAGCTTTATAATCTTGTGGAGAAGCTTAGAAAATGGTCAGTAACTTTTTTTTTTTTTTGTTCTGCTTGCATCCATGAAATGTTAGGGTTTCAGCTCTTTCTTTTGTATGCTGATTTAGTTTAGTCTCTTGTTTTTTCATGTAATTCAAATTGACTTATGTTGACATTATGAATTTGAGAATATTCTATTAAATATGTAGCTCAATTCTTTATCAGCTGCTAATTCTGTGTTGCTATTCGTTCGTTTTCGAATTTTAAAGTTACTGAAAGGATATCTTGTATTCAAGAATTTTCTGGATCAGCTTATAATTCATTATTGGTGTAGGGGAACTTGATCTTTTTTCTCTCTTGCATGTAATTCTTTGGAATGAACAAGTTGTGAATCGCTGTATTTGTTTCTACTTCTTGTAATGCTATTGTTGTTATCTTTTTATTCATTTTGCTATGATGCTTCTTCCTTCAACTGTATGTTAGAGTCTATCGTGAAAACGTGAATTATCTTGCTTATTGATGATCATATTTTTTCTGGGTTTTGTAGGCTACTTTAGCCGATTCTTTTCTAGCAGATCTTGATGAACTCTCTGATGAAGACAATTTTCCGGTGAGCAAATGCATTTTACCTTCCTATCGGGAAAGTAAATTCCATAATCATGTTAGTAGTAGTGTGAACCAATCAATAGTTACTGAATGATTACAGGATGAGGAAGATGTTGATGCTGAAAATATGGAAGAGGATATTGATGGAGACCTTGCCGACCTAGAAAGCCTTAATTATGATGATCTTGATAATGTCTCAAAATTGCAGAAATCACAGAGATACAGTGATATAATGCAGGTTAGTTCACATGAAAGTTTCCCTTATCACTTCGGCATACCTAGTGGCTTTAACGGAAAGGGTTTGGTAAAAGGCGAGTAAATACATTGGAACTCTGTTTTGTCTATTATGCTTACCTTTTGTTGACTGATAAGTATGGGTTACTATTTAATCATTGGACTTTTTTCTCTGTGATAATTCATTGCTTTTAAATAATGCTATTGGAATTTTGATGCATTTTCTAATTTACTATTGAAAACCATGTTGTTCCTTGAACCAAAACTCGTCTCCTTGAAAGGTTAATTTTTATTGCTCATTACATGTTGGATTTCATGATGCTTTATCTCCAGATCATTAAGTTTCTGCAGACTGATAAGTTATAGGTTATCATATAATTATTTGGATTTTACAGAAAGTGGAAGATGCATTACAGAAGGATTCTAATATCTCAAATCAAGGATTAGTATTGGAAGATGATCCTGAGTACCAACTGATTGTGGAGTGTAATGCTTTGTCGGTGGATATTGAGAATGAAATTATTATTATTCACAATTTTATACGTGATAAATATCGATTGAAATTTCCAGAGCTTGAATCACTTGTGCACCACCCAATCGATTATGCTCGAGTTGTTAAGAAGATTGGCAATGAAATGGATTTGACTCTTGTAGACTTAGAAGGGCTTTTACCCTCTGCTGTCATTATGGTTGTCTCAGTTACAGCATCTACCACAAGTGGCAAGCCACTTCCAGAGGAAATTCTCCAGAAAACAATTGATGCATGTGACCGGGCTCTTGCTTTAGATTCAGCAAAGAAAATGGTTCTTAACTTTGTTGAAAGTAGAATGGGGTATATTGCACCAAATCTTTCAGCCATAGTTGGAAGTGCTGTTGCAGCAAAACTAATGGGAACTGCTGGTGGGCTTGCTTCTTTAGCTAAGATGCCTGCTTGCAATGTCCAGCTTCTTGGTGCGAAGAAAAAGAACCTTGCTGGGTTTTCCACAGCAACTTCACAGTTTCGGTTAGGTTACATTGAGCAAACAGAGATATTCCAATCAACACCTCCCCATTTGAAGATGCGTGCTTGTCGACTTGTATCTGCAAAATCAACACTTGCAGCACGAGTTGACTCCACCAGGGGCGATCCTACTGGGAAGACTGGAAGAGTTTTTAAAGATGAGATCCTTAAGAAAATTGAGAAGTGGCAAGAACCTCCACCAGCTAAACAACCAAAACCTCTTCCGGTCCCTGATTCTGAACCCAAAAAGAAGAGAGGTGGCCGTCGACTAAGGAAGATGAAGGAAAGGTACTGATATTTTTTTCCATCTCTCTAACAATCAAAGGTTTAATTGTTTCACTTTTCGGTTGATCATGGATCAATATGACTTCCTTTGTAGATATGCAACAACAGAGATGAGGAAGCTAGCCAACAGGATGCAGTTTGGGGTGCCTGAAGAGAGTTCTTTAGGCAAGGCTTTCGTTCATTTTTATAATAATCTGGATTGGTTAAATTTGTATTCTTGCTTATGCTTCATTCCATTTACATTAGTATAAGATGCAATGCTGTGCTGTTCGATAAGCTTGAGTTGCCAAGTTGTTTGTCTTGTGAACCAGAAAACAGTCATAATTGCAAGTAATACTACTATAACCATCTTGGGTCTTTCCTCTTTCAGGAATATTGGCATGCCAAAAGTAGTTGTATTTTCATCTGTATTTAGAAATTTGTTCTGGATGGAATAAGTAATTATTTGAAGCATTCTCCAATAAGATTCTGAATAAAGATTTCATTCTCTTCATCCTTTCATACAACAGGAGATGGATTAGGGGAAGGGTATGGAATGCTTGGTCAGGCTGGGAGTGGCAAGCTGCGTGTGTCAGCTGCTCAGAGCAAGCTTGCCGCAAAAGTTGTTAAGAAGTAAGTCAAGTCATTGAGCTGATATTAATATTTTCATACCCCTGGAAAATAGAAAATGAAAAATAGAAGTAGATGAGAAAGAGGAGATACTTGCAGCCTAAAATCTGCCCGTTTATCTGTTTTTGTTGACTTATTTTCTCAATTTACTTTTAGGTTCAAGGAAAAACGTTATGGAAGCAGTGGTGCTACGTCTGGCCTGACGTCCAGTTTAGCATTTACACCTGTACAAGTCAGTATTTCTTGCCTTCCTCACCAGTTCCTCCTATGTTATCTAATGTTCGCCTAGAAAGTAATTGTTCTTTTTTTTCATGATACTGCCTGGATACTTAATGTCGGGGTTGTGAGAATTTCATATTTTCGTTGCAGGGGATTGAGCTGTCGAATCCTCAGGCTCATATGAATCAACTAGGCAGTGGAACTCAAAGCACCTACTTTTCTGAAACGGGAACATTTTCAAAAATCAGGAAAAACTAA

mRNA sequence

TGCATCGGCGAACGGCGGTCGTAAAATTCAATTGTTATCTCTTCCTCTGTTTCTGACTATTTCCGTCGATCACAAGCCATTGCCAGTTTCTCCTCTTTCTTTCGTCGGCGTTCTTTCTGATCGTTCTGAGTGTGAATTTTATCTCGTCGGTTACCTTGCTGTAACATTTGGAGAGTATATTTCATCCGAGCTATTCTTCAACTTGGTGCTGAATTGATTACGACCAAGACAACCAGGGAAGCTTTATAATCTTGTGGAGAAGCTTAGAAAATGGCTACTTTAGCCGATTCTTTTCTAGCAGATCTTGATGAACTCTCTGATGAAGACAATTTTCCGGATGAGGAAGATGTTGATGCTGAAAATATGGAAGAGGATATTGATGGAGACCTTGCCGACCTAGAAAGCCTTAATTATGATGATCTTGATAATGTCTCAAAATTGCAGAAATCACAGAGATACAGTGATATAATGCAGAAAGTGGAAGATGCATTACAGAAGGATTCTAATATCTCAAATCAAGGATTAGTATTGGAAGATGATCCTGAGTACCAACTGATTGTGGAGTGTAATGCTTTGTCGGTGGATATTGAGAATGAAATTATTATTATTCACAATTTTATACGTGATAAATATCGATTGAAATTTCCAGAGCTTGAATCACTTGTGCACCACCCAATCGATTATGCTCGAGTTGTTAAGAAGATTGGCAATGAAATGGATTTGACTCTTGTAGACTTAGAAGGGCTTTTACCCTCTGCTGTCATTATGGTTGTCTCAGTTACAGCATCTACCACAAGTGGCAAGCCACTTCCAGAGGAAATTCTCCAGAAAACAATTGATGCATGTGACCGGGCTCTTGCTTTAGATTCAGCAAAGAAAATGGTTCTTAACTTTGTTGAAAGTAGAATGGGGTATATTGCACCAAATCTTTCAGCCATAGTTGGAAGTGCTGTTGCAGCAAAACTAATGGGAACTGCTGGTGGGCTTGCTTCTTTAGCTAAGATGCCTGCTTGCAATGTCCAGCTTCTTGGTGCGAAGAAAAAGAACCTTGCTGGGTTTTCCACAGCAACTTCACAGTTTCGGTTAGGTTACATTGAGCAAACAGAGATATTCCAATCAACACCTCCCCATTTGAAGATGCGTGCTTGTCGACTTGTATCTGCAAAATCAACACTTGCAGCACGAGTTGACTCCACCAGGGGCGATCCTACTGGGAAGACTGGAAGAGTTTTTAAAGATGAGATCCTTAAGAAAATTGAGAAGTGGCAAGAACCTCCACCAGCTAAACAACCAAAACCTCTTCCGGTCCCTGATTCTGAACCCAAAAAGAAGAGAGGTGGCCGTCGACTAAGGAAGATGAAGGAAAGATATGCAACAACAGAGATGAGGAAGCTAGCCAACAGGATGCAGTTTGGGGTGCCTGAAGAGAGTTCTTTAGGAGATGGATTAGGGGAAGGGTATGGAATGCTTGGTCAGGCTGGGAGTGGCAAGCTGCGTGTGTCAGCTGCTCAGAGCAAGCTTGCCGCAAAAGTTGTTAAGAAGTTCAAGGAAAAACGTTATGGAAGCAGTGGTGCTACGTCTGGCCTGACGTCCAGTTTAGCATTTACACCTGTACAAGGGATTGAGCTGTCGAATCCTCAGGCTCATATGAATCAACTAGGCAGTGGAACTCAAAGCACCTACTTTTCTGAAACGGGAACATTTTCAAAAATCAGGAAAAACTAA

Coding sequence (CDS)

ATGGCTACTTTAGCCGATTCTTTTCTAGCAGATCTTGATGAACTCTCTGATGAAGACAATTTTCCGGATGAGGAAGATGTTGATGCTGAAAATATGGAAGAGGATATTGATGGAGACCTTGCCGACCTAGAAAGCCTTAATTATGATGATCTTGATAATGTCTCAAAATTGCAGAAATCACAGAGATACAGTGATATAATGCAGAAAGTGGAAGATGCATTACAGAAGGATTCTAATATCTCAAATCAAGGATTAGTATTGGAAGATGATCCTGAGTACCAACTGATTGTGGAGTGTAATGCTTTGTCGGTGGATATTGAGAATGAAATTATTATTATTCACAATTTTATACGTGATAAATATCGATTGAAATTTCCAGAGCTTGAATCACTTGTGCACCACCCAATCGATTATGCTCGAGTTGTTAAGAAGATTGGCAATGAAATGGATTTGACTCTTGTAGACTTAGAAGGGCTTTTACCCTCTGCTGTCATTATGGTTGTCTCAGTTACAGCATCTACCACAAGTGGCAAGCCACTTCCAGAGGAAATTCTCCAGAAAACAATTGATGCATGTGACCGGGCTCTTGCTTTAGATTCAGCAAAGAAAATGGTTCTTAACTTTGTTGAAAGTAGAATGGGGTATATTGCACCAAATCTTTCAGCCATAGTTGGAAGTGCTGTTGCAGCAAAACTAATGGGAACTGCTGGTGGGCTTGCTTCTTTAGCTAAGATGCCTGCTTGCAATGTCCAGCTTCTTGGTGCGAAGAAAAAGAACCTTGCTGGGTTTTCCACAGCAACTTCACAGTTTCGGTTAGGTTACATTGAGCAAACAGAGATATTCCAATCAACACCTCCCCATTTGAAGATGCGTGCTTGTCGACTTGTATCTGCAAAATCAACACTTGCAGCACGAGTTGACTCCACCAGGGGCGATCCTACTGGGAAGACTGGAAGAGTTTTTAAAGATGAGATCCTTAAGAAAATTGAGAAGTGGCAAGAACCTCCACCAGCTAAACAACCAAAACCTCTTCCGGTCCCTGATTCTGAACCCAAAAAGAAGAGAGGTGGCCGTCGACTAAGGAAGATGAAGGAAAGATATGCAACAACAGAGATGAGGAAGCTAGCCAACAGGATGCAGTTTGGGGTGCCTGAAGAGAGTTCTTTAGGAGATGGATTAGGGGAAGGGTATGGAATGCTTGGTCAGGCTGGGAGTGGCAAGCTGCGTGTGTCAGCTGCTCAGAGCAAGCTTGCCGCAAAAGTTGTTAAGAAGTTCAAGGAAAAACGTTATGGAAGCAGTGGTGCTACGTCTGGCCTGACGTCCAGTTTAGCATTTACACCTGTACAAGGGATTGAGCTGTCGAATCCTCAGGCTCATATGAATCAACTAGGCAGTGGAACTCAAAGCACCTACTTTTCTGAAACGGGAACATTTTCAAAAATCAGGAAAAACTAA

Protein sequence

MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKSQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLASLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSKIRKN
Homology
BLAST of CmaCh18G008060 vs. ExPASy Swiss-Prot
Match: Q8RXN6 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Arabidopsis thaliana OX=3702 GN=PRP31 PE=1 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 3.2e-198
Identity = 373/485 (76.91%), Postives = 421/485 (86.80%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATL DSFLADLDELSD +   DE D D    EED+D D+ADLE+LNYDDLDNVSKLQKS
Sbjct: 1   MATLEDSFLADLDELSDNEAELDENDGDVGKEEEDVDMDMADLETLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIM KVE+AL KDS+ + +G VLEDDPEY+LIV+CN LSVDIENEI+I+HNFI+DK
Sbjct: 61  QRYADIMHKVEEALGKDSDGAEKGTVLEDDPEYKLIVDCNQLSVDIENEIVIVHNFIKDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           Y+LKF ELESLVHHPIDYA VVKKIGNE DL LVDL  LLPSA+IMVVSVTA TT G  L
Sbjct: 121 YKLKFQELESLVHHPIDYACVVKKIGNETDLALVDLADLLPSAIIMVVSVTALTTKGSAL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PE++LQK ++ACDRAL LDSA+K VL FVES+MG IAPNLSAIVGSAVAAKLMGTAGGL+
Sbjct: 181 PEDVLQKVLEACDRALDLDSARKKVLEFVESKMGSIAPNLSAIVGSAVAAKLMGTAGGLS 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300
           +LAKMPACNVQ+LG K+KNLAGFS+ATSQ R+GY+EQTEI+QSTPP L+ RA RLV+AKS
Sbjct: 241 ALAKMPACNVQVLGHKRKNLAGFSSATSQSRVGYLEQTEIYQSTPPGLQARAGRLVAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVD+TRGDP G +G+ F++EI KKIEKWQEPPPA+QPKPLPVPDSEPKK+RGGRRL
Sbjct: 301 TLAARVDATRGDPLGISGKAFREEIRKKIEKWQEPPPARQPKPLPVPDSEPKKRRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLA-- 420
           RKMKERY  T+MRKLANRM FG PEESSLGDGLGEGYGMLGQAGS +LRVS+  SKL   
Sbjct: 361 RKMKERYQVTDMRKLANRMAFGTPEESSLGDGLGEGYGMLGQAGSNRLRVSSVPSKLKIN 420

Query: 421 AKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTF 480
           AKV KK KE++Y     TSGLTSSLAFTPVQGIEL NPQ  +  LGSGTQSTYFSE+GTF
Sbjct: 421 AKVAKKLKERQYAGGATTSGLTSSLAFTPVQGIELCNPQQALG-LGSGTQSTYFSESGTF 480

Query: 481 SKIRK 484
           SK++K
Sbjct: 481 SKLKK 484

BLAST of CmaCh18G008060 vs. ExPASy Swiss-Prot
Match: Q8CCF0 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Mus musculus OX=10090 GN=Prpf31 PE=1 SV=3)

HSP 1 Score: 386.3 bits (991), Expect = 4.9e-106
Identity = 234/496 (47.18%), Postives = 332/496 (66.94%), Query Frame = 0

Query: 3   TLADSFLADLDELSDED---NFPDEEDVDA-ENMEEDIDGDLADLESLNYDDLDNVSKLQ 62
           +LAD  LADL+E ++E+   ++ +EE+  A E+++E+   DL+       D + +++KL 
Sbjct: 2   SLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSG------DSVKSIAKLW 61

Query: 63  KSQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIR 122
            S+ +++IM K+E+ + K +N+S     +E  PEY++IV+ N L+V+IENE+ IIH FIR
Sbjct: 62  DSKMFAEIMMKIEEYISKQANVSEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIR 121

Query: 123 DKYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTS 182
           DKY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT 
Sbjct: 122 DKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQ 181

Query: 183 GKPLPEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTA 242
           G+ L +E L++  +ACD AL L+++K  +  +VESRM +IAPNLS I+G++ AAK+MG A
Sbjct: 182 GQQLSDEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVA 241

Query: 243 GGLASLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLV 302
           GGL +L+KMPACN+ LLGA++K L+GFS+ +     GYI  ++I QS PP L+ +A RLV
Sbjct: 242 GGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLV 301

Query: 303 SAKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRG 362
           +AK TLAARVDS      GK G   KDEI +K +KWQEPPP KQ KPLP P    +KKRG
Sbjct: 302 AAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRG 361

Query: 363 GRRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLR---VSAA 422
           GRR RKMKER   TE+RK ANRM FG  EE +  + LG   G LG++GSG++R   V+ A
Sbjct: 362 GRRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRVRQTQVNEA 421

Query: 423 QSKLAAKVVKKFKEKR---YGSSGA----TSGLTSSLAFTPVQGIELSNPQAHMNQLGSG 482
                +K +++  +K+   YG        +SG  SS+AFTP+QG+E+ NPQA   ++   
Sbjct: 422 TKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEA 481

BLAST of CmaCh18G008060 vs. ExPASy Swiss-Prot
Match: Q5U5C5 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus laevis OX=8355 GN=prpf31 PE=2 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.4e-105
Identity = 237/495 (47.88%), Postives = 327/495 (66.06%), Query Frame = 0

Query: 3   TLADSFLADLDELS--DEDNFPDEEDVDA-ENMEEDIDGDLADLESLNYDDLDNVSKLQK 62
           +LAD  LADL+E +  +E+N  DE+D++  E ++E++  D      LN + + +++KL  
Sbjct: 2   SLADELLADLEEAAEEEEENLIDEDDLETIEEVDEEMQVD------LNAESVKSIAKLSD 61

Query: 63  SQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRD 122
           S+ +S+I+ K+E  +QK    S     +E  PEY++IV+ N L+V+IENE+ IIH FIRD
Sbjct: 62  SKLFSEILLKIEGYIQKQPKASEVMGPVEAAPEYKVIVDANNLTVEIENELNIIHKFIRD 121

Query: 123 KYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTSG 182
           KY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT G
Sbjct: 122 KYSKRFPELESLVPNALDYIRTVKELGNNLDKCKNNENLQQILTNATIMVVSVTASTTQG 181

Query: 183 KPLPEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAG 242
           + L +E L++  +ACD AL L+ +K  +  +VESRM +IAPNLS IVG++ AAK+MG AG
Sbjct: 182 QQLTDEELERIEEACDMALELNQSKHRIYEYVESRMSFIAPNLSIIVGASTAAKIMGIAG 241

Query: 243 GLASLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVS 302
           GL +L+KMPACNV LLGA++K L GFS+ +     GYI  +EI QS P  L  +A RLVS
Sbjct: 242 GLTNLSKMPACNVMLLGAQRKTLTGFSSTSVLPHTGYIYHSEIVQSLPSDLHRKAARLVS 301

Query: 303 AKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGG 362
           AK TLA+RVDS   +P GK G   K+EI +K +KWQEPPP KQ KPLP P    +KKRGG
Sbjct: 302 AKCTLASRVDSFHENPEGKIGYDLKEEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGG 361

Query: 363 RRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLR---VSAAQ 422
           RR RKMKER   TE+RK ANRM FG  EE +  + LG   G LG++GSG++R   V+ A 
Sbjct: 362 RRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRIRQAQVNEAT 421

Query: 423 SKLAAKVVKKFKEKR---YGSSGA----TSGLTSSLAFTPVQGIELSNPQAHMNQLGSGT 482
               +K +++  +K+   YG        +SG  SS+AFTP+QG+E+ NPQA   ++    
Sbjct: 422 KARISKTLQRTLQKQSVVYGGKSTVRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEAN 481

BLAST of CmaCh18G008060 vs. ExPASy Swiss-Prot
Match: Q8WWY3 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Homo sapiens OX=9606 GN=PRPF31 PE=1 SV=2)

HSP 1 Score: 383.3 bits (983), Expect = 4.2e-105
Identity = 234/496 (47.18%), Postives = 329/496 (66.33%), Query Frame = 0

Query: 3   TLADSFLADLDELSDED---NFPDEEDVDA-ENMEEDIDGDLADLESLNYDDLDNVSKLQ 62
           +LAD  LADL+E ++E+   ++ +EE+  A E+++E+   DL+       D +  ++KL 
Sbjct: 2   SLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSG------DSVKTIAKLW 61

Query: 63  KSQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIR 122
            S+ +++IM K+E+ + K +  S     +E  PEY++IV+ N L+V+IENE+ IIH FIR
Sbjct: 62  DSKMFAEIMMKIEEYISKQAKASEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIR 121

Query: 123 DKYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTS 182
           DKY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT 
Sbjct: 122 DKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQ 181

Query: 183 GKPLPEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTA 242
           G+ L EE L++  +ACD AL L+++K  +  +VESRM +IAPNLS I+G++ AAK+MG A
Sbjct: 182 GQQLSEEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVA 241

Query: 243 GGLASLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLV 302
           GGL +L+KMPACN+ LLGA++K L+GFS+ +     GYI  ++I QS PP L+ +A RLV
Sbjct: 242 GGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLV 301

Query: 303 SAKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRG 362
           +AK TLAARVDS      GK G   KDEI +K +KWQEPPP KQ KPLP P    +KKRG
Sbjct: 302 AAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRG 361

Query: 363 GRRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLR---VSAA 422
           GRR RKMKER   TE+RK ANRM FG  EE +  + LG   G LG++GSG++R   V+ A
Sbjct: 362 GRRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRVRQTQVNEA 421

Query: 423 QSKLAAKVVKKFKEKR---YGSSGA----TSGLTSSLAFTPVQGIELSNPQAHMNQLGSG 482
                +K +++  +K+   YG        +SG  SS+AFTP+QG+E+ NPQA   ++   
Sbjct: 422 TKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEA 481

BLAST of CmaCh18G008060 vs. ExPASy Swiss-Prot
Match: Q6NVP6 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus tropicalis OX=8364 GN=prpf31 PE=2 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 3.5e-104
Identity = 234/495 (47.27%), Postives = 326/495 (65.86%), Query Frame = 0

Query: 3   TLADSFLADLDELS--DEDNFPDEEDVDA-ENMEEDIDGDLADLESLNYDDLDNVSKLQK 62
           +LAD  LADL+E +  +E+N  DE+D++  E ++E++  D      LN + + +++KL  
Sbjct: 2   SLADELLADLEEAAEEEEENLIDEDDLETIEEVQEEMQVD------LNAESVKSIAKLSD 61

Query: 63  SQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRD 122
           S+ +S+I+ K++  ++K    S     +E  PEY++IV+ N L+V+IENE+ IIH FIRD
Sbjct: 62  SKLFSEILLKIDGYIKKQPKASEVMGPVEAAPEYKVIVDANNLTVEIENELNIIHKFIRD 121

Query: 123 KYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTSG 182
           KY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT G
Sbjct: 122 KYSKRFPELESLVPNALDYIRTVKELGNNLDKCKNNENLQQILTNATIMVVSVTASTTQG 181

Query: 183 KPLPEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAG 242
           + L +E L++  +ACD AL L+ +K  +  +VESRM +IAPNLS IVG++ AAK+MG AG
Sbjct: 182 QQLTDEELERIEEACDMALELNQSKHRIYEYVESRMSFIAPNLSIIVGASTAAKIMGIAG 241

Query: 243 GLASLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVS 302
           GL +L+KMPACNV LLGA++K L+GFS+ +     GYI  ++I QS PP L  +A RLVS
Sbjct: 242 GLTNLSKMPACNVMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLHRKAARLVS 301

Query: 303 AKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGG 362
           AK TLAARVDS      GK G   K+EI +K +KWQEPPP KQ KPLP P    +KKRGG
Sbjct: 302 AKCTLAARVDSFHESSEGKVGYDLKEEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGG 361

Query: 363 RRLRKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLR---VSAAQ 422
           RR RKMKER   TE+RK ANRM F   EE +  + LG   G LG++GSG++R   V+ A 
Sbjct: 362 RRYRKMKERLGLTEIRKQANRMSFAEIEEDAYQEDLGFSLGHLGKSGSGRIRQAQVNEAT 421

Query: 423 SKLAAKVVKKFKEKR---YGSSGA----TSGLTSSLAFTPVQGIELSNPQAHMNQLGSGT 482
               +K +++  +K+   YG        +SG  SS+AFTP+QG+E+ NPQA   ++    
Sbjct: 422 KARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEAN 481

BLAST of CmaCh18G008060 vs. ExPASy TrEMBL
Match: A0A6J1HSU1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita maxima OX=3661 GN=LOC111466452 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 1.8e-260
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300
           SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK 480

Query: 481 IRKN 485
           IRKN
Sbjct: 481 IRKN 484

BLAST of CmaCh18G008060 vs. ExPASy TrEMBL
Match: A0A6J1G0W2 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita moschata OX=3662 GN=LOC111449657 PE=3 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 1.1e-257
Identity = 479/484 (98.97%), Postives = 481/484 (99.38%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300
           SLAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRLVSAKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAH+NQLG GTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSK 480

Query: 481 IRKN 485
           IRKN
Sbjct: 481 IRKN 484

BLAST of CmaCh18G008060 vs. ExPASy TrEMBL
Match: A0A6J1DBC9 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019139 PE=3 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.6e-253
Identity = 471/484 (97.31%), Postives = 480/484 (99.17%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNF DEEDVDAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKVE ALQKDSNISNQGLVLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRALALDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA
Sbjct: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRL++AKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAH+NQLGSGTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK 480

Query: 481 IRKN 485
           IRKN
Sbjct: 481 IRKN 484

BLAST of CmaCh18G008060 vs. ExPASy TrEMBL
Match: A0A6J1KRI6 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497587 PE=3 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.4e-251
Identity = 465/484 (96.07%), Postives = 475/484 (98.14%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNF DEED DAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKV DAL+ DSN+SNQG VLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRAL LDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLV 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRL++AKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAH+NQLG GTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSK 480

Query: 481 IRKN 485
           IRKN
Sbjct: 481 IRKN 484

BLAST of CmaCh18G008060 vs. ExPASy TrEMBL
Match: A0A6J1GXP7 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458320 PE=3 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.4e-251
Identity = 465/484 (96.07%), Postives = 475/484 (98.14%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNF DEED DAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKV DAL+ DSN+SNQG VLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRAL LDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLV 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRL++AKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAH+NQLG GTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSK 480

Query: 481 IRKN 485
           IRKN
Sbjct: 481 IRKN 484

BLAST of CmaCh18G008060 vs. NCBI nr
Match: XP_022966885.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita maxima])

HSP 1 Score: 907.9 bits (2345), Expect = 3.7e-260
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300
           SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK 480

Query: 481 IRKN 485
           IRKN
Sbjct: 481 IRKN 484

BLAST of CmaCh18G008060 vs. NCBI nr
Match: XP_022945419.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita moschata] >XP_022945420.1 U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita moschata] >KAG6573747.1 U4/U6 small nuclear ribonucleoprotein Prp31-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 898.7 bits (2321), Expect = 2.3e-257
Identity = 479/484 (98.97%), Postives = 481/484 (99.38%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300
           SLAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRLVSAKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAH+NQLG GTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSK 480

Query: 481 IRKN 485
           IRKN
Sbjct: 481 IRKN 484

BLAST of CmaCh18G008060 vs. NCBI nr
Match: KAG7012823.1 (U4/U6 small nuclear ribonucleoprotein Prp31-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 884.8 bits (2285), Expect = 3.4e-253
Identity = 479/509 (94.11%), Postives = 481/509 (94.50%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300
           SLAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRLVSAKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLG-------------------------DGLGE 420
           RKMKERYATTEMRKLANRMQFGVPEESSLG                         DGLGE
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420

Query: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480
           GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN
Sbjct: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480

Query: 481 PQAHMNQLGSGTQSTYFSETGTFSKIRKN 485
           PQAH+NQLG GTQSTYFSETGTFSKIRKN
Sbjct: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 509

BLAST of CmaCh18G008060 vs. NCBI nr
Match: XP_022151138.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Momordica charantia])

HSP 1 Score: 884.8 bits (2285), Expect = 3.4e-253
Identity = 471/484 (97.31%), Postives = 480/484 (99.17%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNF DEEDVDAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKVE ALQKDSNISNQGLVLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRALALDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA
Sbjct: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRL++AKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAH+NQLGSGTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGSGTQSTYFSETGTFSK 480

Query: 481 IRKN 485
           IRKN
Sbjct: 481 IRKN 484

BLAST of CmaCh18G008060 vs. NCBI nr
Match: XP_022956658.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita moschata] >XP_023004221.1 U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita maxima] >XP_023526610.1 U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita pepo subsp. pepo] >KAG6601489.1 U4/U6 small nuclear ribonucleoprotein Prp31-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 877.1 bits (2265), Expect = 7.0e-251
Identity = 465/484 (96.07%), Postives = 475/484 (98.14%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNF DEED DAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKV DAL+ DSN+SNQG VLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PEEILQKTIDACDRAL LDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLV 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRL++AKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLAAK 420

Query: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTFSK 480
           VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAH+NQLG GTQSTYFSETGTFSK
Sbjct: 421 VVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSK 480

Query: 481 IRKN 485
           IRKN
Sbjct: 481 IRKN 484

BLAST of CmaCh18G008060 vs. TAIR 10
Match: AT1G60170.1 (pre-mRNA processing ribonucleoprotein binding region-containing protein )

HSP 1 Score: 692.6 bits (1786), Expect = 2.3e-199
Identity = 373/485 (76.91%), Postives = 421/485 (86.80%), Query Frame = 0

Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATL DSFLADLDELSD +   DE D D    EED+D D+ADLE+LNYDDLDNVSKLQKS
Sbjct: 1   MATLEDSFLADLDELSDNEAELDENDGDVGKEEEDVDMDMADLETLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIM KVE+AL KDS+ + +G VLEDDPEY+LIV+CN LSVDIENEI+I+HNFI+DK
Sbjct: 61  QRYADIMHKVEEALGKDSDGAEKGTVLEDDPEYKLIVDCNQLSVDIENEIVIVHNFIKDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           Y+LKF ELESLVHHPIDYA VVKKIGNE DL LVDL  LLPSA+IMVVSVTA TT G  L
Sbjct: 121 YKLKFQELESLVHHPIDYACVVKKIGNETDLALVDLADLLPSAIIMVVSVTALTTKGSAL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240
           PE++LQK ++ACDRAL LDSA+K VL FVES+MG IAPNLSAIVGSAVAAKLMGTAGGL+
Sbjct: 181 PEDVLQKVLEACDRALDLDSARKKVLEFVESKMGSIAPNLSAIVGSAVAAKLMGTAGGLS 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300
           +LAKMPACNVQ+LG K+KNLAGFS+ATSQ R+GY+EQTEI+QSTPP L+ RA RLV+AKS
Sbjct: 241 ALAKMPACNVQVLGHKRKNLAGFSSATSQSRVGYLEQTEIYQSTPPGLQARAGRLVAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVD+TRGDP G +G+ F++EI KKIEKWQEPPPA+QPKPLPVPDSEPKK+RGGRRL
Sbjct: 301 TLAARVDATRGDPLGISGKAFREEIRKKIEKWQEPPPARQPKPLPVPDSEPKKRRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGDGLGEGYGMLGQAGSGKLRVSAAQSKLA-- 420
           RKMKERY  T+MRKLANRM FG PEESSLGDGLGEGYGMLGQAGS +LRVS+  SKL   
Sbjct: 361 RKMKERYQVTDMRKLANRMAFGTPEESSLGDGLGEGYGMLGQAGSNRLRVSSVPSKLKIN 420

Query: 421 AKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHMNQLGSGTQSTYFSETGTF 480
           AKV KK KE++Y     TSGLTSSLAFTPVQGIEL NPQ  +  LGSGTQSTYFSE+GTF
Sbjct: 421 AKVAKKLKERQYAGGATTSGLTSSLAFTPVQGIELCNPQQALG-LGSGTQSTYFSESGTF 480

Query: 481 SKIRK 484
           SK++K
Sbjct: 481 SKLKK 484

BLAST of CmaCh18G008060 vs. TAIR 10
Match: AT1G70400.1 (CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thaliana protein match is: pre-mRNA processing ribonucleoprotein binding region-containing protein (TAIR:AT1G60170.1); Has 479 Blast hits to 479 proteins in 178 species: Archae - 0; Bacteria - 0; Metazoa - 125; Fungi - 138; Plants - 124; Viruses - 0; Other Eukaryotes - 92 (source: NCBI BLink). )

HSP 1 Score: 212.2 bits (539), Expect = 9.1e-55
Identity = 117/172 (68.02%), Postives = 138/172 (80.23%), Query Frame = 0

Query: 39  DLADLESLNYDDLDNVSKLQKSQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVE 98
           D+ +L +L YDDLD+VSKLQKS+RY+DIMQ+VE+AL        +G VLE     +LIV+
Sbjct: 2   DMTELNTLTYDDLDSVSKLQKSRRYADIMQQVEEAL--------EGSVLE---YKKLIVD 61

Query: 99  CNALSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEG 158
           C  L VDIENEI+I+ NFIRDKYR+KF ELE LV HPIDYARVVK+IGNEMDL LVDLEG
Sbjct: 62  CKQLLVDIENEIVIVQNFIRDKYRVKFQELELLVPHPIDYARVVKRIGNEMDLKLVDLEG 121

Query: 159 LLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKKMVLNFVE 211
           LLPSA+IMV+ VTA TT G  LPE++L KTIDACDRAL LDSA+K VL FV+
Sbjct: 122 LLPSAMIMVLLVTALTTKGNQLPEDVLLKTIDACDRALDLDSARKKVLEFVD 162

BLAST of CmaCh18G008060 vs. TAIR 10
Match: AT1G70400.2 (CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thaliana protein match is: pre-mRNA processing ribonucleoprotein binding region-containing protein (TAIR:AT1G60170.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 204.9 bits (520), Expect = 1.4e-52
Identity = 113/165 (68.48%), Postives = 133/165 (80.61%), Query Frame = 0

Query: 39  DLADLESLNYDDLDNVSKLQKSQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVE 98
           D+ +L +L YDDLD+VSKLQKS+RY+DIMQ+VE+AL        +G VLE     +LIV+
Sbjct: 2   DMTELNTLTYDDLDSVSKLQKSRRYADIMQQVEEAL--------EGSVLE---YKKLIVD 61

Query: 99  CNALSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEG 158
           C  L VDIENEI+I+ NFIRDKYR+KF ELE LV HPIDYARVVK+IGNEMDL LVDLEG
Sbjct: 62  CKQLLVDIENEIVIVQNFIRDKYRVKFQELELLVPHPIDYARVVKRIGNEMDLKLVDLEG 121

Query: 159 LLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKK 204
           LLPSA+IMV+ VTA TT G  LPE++L KTIDACDRAL LDSA+K
Sbjct: 122 LLPSAMIMVLLVTALTTKGNQLPEDVLLKTIDACDRALDLDSARK 155

BLAST of CmaCh18G008060 vs. TAIR 10
Match: AT1G70400.3 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: egg cell; CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thaliana protein match is: pre-mRNA processing ribonucleoprotein binding region-containing protein (TAIR:AT1G60170.1); Has 484 Blast hits to 484 proteins in 190 species: Archae - 0; Bacteria - 0; Metazoa - 149; Fungi - 134; Plants - 124; Viruses - 0; Other Eukaryotes - 77 (source: NCBI BLink). )

HSP 1 Score: 204.9 bits (520), Expect = 1.4e-52
Identity = 113/165 (68.48%), Postives = 133/165 (80.61%), Query Frame = 0

Query: 39  DLADLESLNYDDLDNVSKLQKSQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVE 98
           D+ +L +L YDDLD+VSKLQKS+RY+DIMQ+VE+AL        +G VLE     +LIV+
Sbjct: 2   DMTELNTLTYDDLDSVSKLQKSRRYADIMQQVEEAL--------EGSVLE---YKKLIVD 61

Query: 99  CNALSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEG 158
           C  L VDIENEI+I+ NFIRDKYR+KF ELE LV HPIDYARVVK+IGNEMDL LVDLEG
Sbjct: 62  CKQLLVDIENEIVIVQNFIRDKYRVKFQELELLVPHPIDYARVVKRIGNEMDLKLVDLEG 121

Query: 159 LLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKK 204
           LLPSA+IMV+ VTA TT G  LPE++L KTIDACDRAL LDSA+K
Sbjct: 122 LLPSAMIMVLLVTALTTKGNQLPEDVLLKTIDACDRALDLDSARK 155

BLAST of CmaCh18G008060 vs. TAIR 10
Match: AT3G05060.1 (NOP56-like pre RNA processing ribonucleoprotein )

HSP 1 Score: 100.5 bits (249), Expect = 3.8e-21
Identity = 69/239 (28.87%), Postives = 118/239 (49.37%), Query Frame = 0

Query: 79  NISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDY 138
           +++   L    D    +I++   L  D++ E+      +R+ Y   FPEL  ++   I Y
Sbjct: 144 SLARYKLKFSSDKVDTMIIQAIGLLDDLDKELNTYAMRVREWYGWHFPELAKIISDNILY 203

Query: 139 ARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALAL 198
           A+ VK +GN ++   +D   +L   +   +   A  + G  + +  L    + CD+ L+L
Sbjct: 204 AKSVKLMGNRVNAAKLDFSEILADEIEADLKDAAVISMGTEVSDLDLLHIRELCDQVLSL 263

Query: 199 DSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLASLAKMPACNVQLLGAKKK 258
              +  + ++++SRM  IAPNL+A+VG  V A+L+   G L +L+K P   VQ+LGA+K 
Sbjct: 264 SEYRAQLYDYLKSRMNTIAPNLTALVGELVGARLISHGGSLLNLSKQPGSTVQILGAEKA 323

Query: 259 NLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKSTLAARVDSTRGDPTGKT 318
                 T  +  + G I    +     P  K +  R ++AK+ LA RVD+  GD    T
Sbjct: 324 LFRALKTKHATPKYGLIFHASLVGQAAPKHKGKISRSLAAKTVLAIRVDAL-GDSQDNT 381

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RXN63.2e-19876.91U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Arabidopsis thaliana OX=3... [more]
Q8CCF04.9e-10647.18U4/U6 small nuclear ribonucleoprotein Prp31 OS=Mus musculus OX=10090 GN=Prpf31 P... [more]
Q5U5C51.4e-10547.88U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus laevis OX=8355 GN=prpf31 ... [more]
Q8WWY34.2e-10547.18U4/U6 small nuclear ribonucleoprotein Prp31 OS=Homo sapiens OX=9606 GN=PRPF31 PE... [more]
Q6NVP63.5e-10447.27U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus tropicalis OX=8364 GN=prp... [more]
Match NameE-valueIdentityDescription
A0A6J1HSU11.8e-260100.00U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1G0W21.1e-25798.97U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita moschata OX=366... [more]
A0A6J1DBC91.6e-25397.31U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Momordica char... [more]
A0A6J1KRI63.4e-25196.07U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita maxi... [more]
A0A6J1GXP73.4e-25196.07U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita mosc... [more]
Match NameE-valueIdentityDescription
XP_022966885.13.7e-260100.00U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita maxima][more]
XP_022945419.12.3e-25798.97U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita moschata] >XP_022... [more]
KAG7012823.13.4e-25394.11U4/U6 small nuclear ribonucleoprotein Prp31-like protein [Cucurbita argyrosperma... [more]
XP_022151138.13.4e-25397.31U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Momordica charan... [more]
XP_022956658.17.0e-25196.07U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita moscha... [more]
Match NameE-valueIdentityDescription
AT1G60170.12.3e-19976.91pre-mRNA processing ribonucleoprotein binding region-containing protein [more]
AT1G70400.19.1e-5568.02CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thalian... [more]
AT1G70400.21.4e-5268.48CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thalian... [more]
AT1G70400.31.4e-5268.48FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT3G05060.13.8e-2128.87NOP56-like pre RNA processing ribonucleoprotein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012976NOSICSMARTSM00931NOSIC_2coord: 95..147
e-value: 6.4E-23
score: 92.1
IPR042239Nop, C-terminal domainGENE3D1.10.246.90Nop domaincoord: 217..342
e-value: 1.9E-40
score: 139.5
IPR019175Prp31 C-terminalPFAMPF09785Prp31_Ccoord: 339..456
e-value: 3.8E-43
score: 147.3
NoneNo IPR availableGENE3D1.10.287.4070coord: 77..216
e-value: 8.5E-57
score: 193.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 456..484
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 330..361
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 456..478
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..36
NoneNo IPR availablePANTHERPTHR13904:SF0U4/U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31coord: 1..481
IPR002687Nop domainPFAMPF01798Nopcoord: 103..331
e-value: 3.2E-73
score: 246.0
IPR002687Nop domainPROSITEPS51358NOPcoord: 216..334
score: 37.259941
IPR027105U4/U6 small nuclear ribonucleoprotein Prp31PANTHERPTHR13904PRE-MRNA SPLICING FACTOR PRP31coord: 1..481
IPR036070Nop domain superfamilySUPERFAMILY89124Nop domaincoord: 88..334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G008060.1CmaCh18G008060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000244 spliceosomal tri-snRNP complex assembly
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0071011 precatalytic spliceosome
cellular_component GO:0005687 U4 snRNP
cellular_component GO:0046540 U4/U6 x U5 tri-snRNP complex
molecular_function GO:0003723 RNA binding