CmaCh18G007460 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh18G007460
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptioncytochrome P450 71B19-like
LocationCma_Chr18: 6699586 .. 6704953 (-)
RNA-Seq ExpressionCmaCh18G007460
SyntenyCmaCh18G007460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGAAAGACCTAGAAGAGTTTCTTCAAGAGAGATTTTGCAGGGAGCTCCATTGATTACAAAGGACTACATTTTGAGTTCTTGCCATTTGGGGGTGGTCGGAGGATATGCTCGGGGTTGGACATGGGAGGTGAAATCTGTGGATTTGGCTTTTGCAAATCTCTTGTACCATTTTGATTGGAAGTTGCCGGAGGGGATGAAGGAGGAGGACTTGGACATGGAAGAGAAATTAGGTTTGAGTCTCACCGTCCACAAAAGTTGCCTCTCGAACTAGTACCAATGTTATACCATCCATGAGTATTAAACTTGAAGTGGAGTCCTCTAATTGTAAAGTATAAGACTTGCCTTATGTATGACGACACGTGACCAGTGACCCTTGCATGTGTCATTTATGCTTATATACTATGATGATGATTATGTGAGCTAGTATGATGTGATGTTATATTATGATATGATGACATGATGACGATGGAGTGTGTTGATGATATTACTCAATATGATGATATTTTATATTAAGATAATGTGTATATACGAGATGACATGCTGCATTATGATGAAGAGTTTTCCGAGGCACAACCCTACACTAGTGTAATGATGATGAATGTATGAAGGCTAATGCATGCATGTTACCTAGAATATTATGTGAATGATGTATAGGGTTGTGTCATAAGAATAATTTAAGATGAAAACTATAGTGACCTCATGCATATTGTGTGTTCATATATATTAGGTTACTTCTCCAATTATGATAATGAGTACAAACACGTACAATATGATGATGATCATCATCATGCCTTTATGATATTCAAGAATTTGCTGACATCCGGACATCCGACTACGAACAACTAATCTCACTATGATGGGTCTAGGGGTGCAAACCACCTGGTGGTCTAATGCTCGCACATATGGATCGTGTGTAGTGAAGTACTACACATCCAATTTTGTCCGGACTAGAGACCACCTCTCTAATGGTTTTAACCATGTCCCTAATCATGAGTTGCATCTTTTGCATTCCCTAACCCAATTAGTACGACCATTTACTAAGTATTTTTTAAAATATTCAAGTCATGGATTATTTCTATTTTTCAATAAATACAAGGCACCCCTATATACTGATGATCACAAGAAAAAAAAAAAAAAATTGCAGTAAAGAATGATGTACACTTAAAAGATAAAAGATAAAAGTAGTAATATAATTTGAAATAATTCAAATTGATCCCAAAACAAGATAACATCCATTCCAATCAATGAAGTTTACCCAATTTTTTTAAAAAAATTCTCCCTAAAAAGTTGTGATTTTTTAAAATTAGGTTTTATTTATCTTTTAAAATGAATTTAATAGATTATTTTTTACTTTTCAAAAGTAAATAAATTAAAAAATTGATATTTGATTTTATTGGATGTCACATGGAAGGTATGGGCCACTTTCCACATCTAATGCATCGAGACTATGGGAAGGTATGGGCCACTTTCCACCTCTAATGCATCTTTTTCGAGTGAGAGGGAATTTTGCCAATTTCTGTATAATAGACGAATACGCAAGCATACGTTTCATGCTTGTTTGAGTGTTTTCTCCTCGTACGGCTCGAGGAAATTCAAAGTTCTTTCAGGAATATCAATACTGTTACAAATAGTAATCATAGAATTGGGTTTTTTCTAAGCGGAGCCTGGATACTTCATTTTATTAGTACAACCGAGAAAACCATAAATTATTCTAATTGATAATATTAATCTGGATACCCCAAAAATTAGATCTAATTCCACTTCACGCTCCAAATTTTTGCATCGAGACAAAAATCTTTGAAGTTGATTTTTGTTATTTATTGTTATTTTCTATTGCAGCTTGTATCATTGAAAAATACAGATCATCTACTAGAAGATTAATAATCAAATCTATCATGCTAATGTTTCAATTTTTGTTTTCCATCTTGAGAGGATGACAGCTTATTGTTTTCATGGAGTGGACCGTCATTTTTTAACATTATTGGTTGATGTTATAAGAATAATATCAATGGACCATCATTTTTTTTTTAAAAATAATTCATTATTTTGGTTCAAATAATATAAGACTTTTGTTTTATTTCTATTTAATCCAAAAGAAGAATCAATGTTTTTTATTCCTAATCTTTTTCCTCTCTTCTTGAACTCTTTCTAAACTTCAATGTCGTTCCTTCCTTTCCCTCCATGGCTTCCCCTCATCCTCTTGAAAATTGCCGGCCGAGACGGCTCGTGCCCTCTTGAAAATTCACGATCTTCAAAGCTGCAACAGATACAAACACATGCTATAAAAAAATTCTCCTACAATTTCCTTGACATTAGGTTTTCACCCTACAGTGATGACTGACGAGAGATGTGCAAAATTTGTATGCTTGAGTTTTTCAATATGAAGAGGGTGTTCTCTTACGAATCCATTAGAAAGCAAGAAGTTGGTTTACTTATTGAATCAATTTCACAATCAGCTTTTTGTCAAGTGGTTGTTGATCTTAGTGTTATTTTTAGAATTGCATTTGGGTAGTGGTTTGAAGGGGATGGATTTCATGAGCATATAAGTCAAGTTCCTGTTGTAGGAGAGGTATTCAATTGGTTTAGTGGTCCAGAAGCTAGACTTGAGAGGGTTTTTAATGAGATAAATTCTCTCTTTCAAGAGGTGATTGATGAACTACACATCCAATTTTGTCCGGACTAAAGACCACCTCTCTAATAGTTTTAACCCTATGTCCCTCCGAATTATGAGTGACATGTGTTTGCATTCCCTAACCCAAATAGTAGAACCACTTACAAAGTATTTTTTAAAATACTAAAGTTGTGGGCTATTTCTATTTTTTAGGTAAATACAAGGCACCCCTATACGCTGACGACATTGCAAGTAGAGACTATACATAGGACAGTGGTATTTCAATTCTTAGATTTTATGTTTGAATAGGGTGTTATTACTTTTAGTATTTAATTTGTTTTATTTAATATTTCATTTATATTACTTTAAAACCTTTTTAATCCTATAAATCCATAACAATTTTTATGAATATGTTTTTGAATTGTTATTTATGCATAAAAAATTATATTACGAAAACCCGTAGCAATTTTCGGTTAGTTAAAATCTAGAGTCGTTGCTTCAATTTTTTTTAATTGAAACTAAAATAAAATAAAATTCAAAATATAAATTTCAAAAAAGGATATTTTAACCTTTTTCTATTATTATGTAACTACTAAACATAATAAACAAATATGTGTTGATTATAAACTTATATTATTATATATTTACTAAATTGATGAAATTTTATAAAGAAAAAAAAATAGGACCACTTTCTCATTTTCCAAAGGGATCTCAAGGTATATGTTTGAAGTTGATTTTAGTTCTTTATTATTCTATTGCCTATTCTATTACCGCTTATATCATTGTCTCAATCTCAATCTCCATCCTTAATTGAAAATCTAAGACACGGTTGGATAAGATCAGACCCTATCAAATTAGATTTAATCATAACTTCTAAACGTGATATCATTTGTAAAAATATAGATTATCTACTAGAAGATTAGTATTCAAATCTATCATGCTAATACCTAGTGTTTCAATTTTTGAATATGACAACTTATGGCTTACATGGAGTGGACAACCATTTTTTTATTTGAATGTTTTGTTTTATGTCTATTTAATCGAGAGAAGAATCACCCTTTTTTATTCGTGATCTTTTTCCTCTTGTTCTTGAGCTCTTTCTAAATTCTAATGTCTTTCCTTCCCTTCCCTCCATGGCTTCCCCTCCTCCTCTTCGTCTCTTCACTTTTCATCATCCTAAAATGGAAAGCAGCTGCTAATAGAAGAAAGCGTAATTTTCCCCCAAGCCCACCGAAGCTTCCAATCATTGGAAACTTGCATCAACTTGGCAAGCTGCCACACAGATCTCTTTGGCGCCTTTCTCAGCTTTACGGTCCTATTATGAGCCTCAACCTAGGTCGTATAGAAACCATCATCATATCATCGACTGAAACTGCTCGTGCCCTCTTGAAAACTCACGATCTTCAAAGTTGCAACAGACCACAAACTCATGCTATGAAAAAATTCACTTACAATTTTCTTGACCTTGGTTTTTCACCCTACAATGATTACTTGCGAGAGATGCGCAAAATTTGTATGCTTGAGTTTTTCAGTATGAAGAGGGTCTTATCTTACAAACCCATTAGAGAGCAAGAAGTTTGTTTGCTTATTGAATCAATTTCACAATCAGCTTCTTGTGGAGCTATTGTTGATCTTAGTGAGAAGTCCATTGCTCTCACTACTGGTGTTATTTTTAGAATTGCATTTGGGAAGCCATTTGAAGGGGATGGATTTCATGAGCTTATAAGTGAAGTTGAAGCCCTGATGGGAAGCTATAGTGCTTCTGAGTTTTTTCCAGTTCCTTTTGTGGGAGAAGTATTTGATTGGTTTAGTGGTCGAGAAGCTAGAATTGAGAAAGTTTTTAATGAGATAAATGCTCTATTTCAAAAGGTGATTGATGAACACCTCTGTCCTGAGAGGCCTAAGCCAGAGCAAGATGATATCATTGATGTGCTTTTGGCTATTGGTAAAAAGCAAGTTCATCCTCACACGGTTGTTATTAACCATGAAAACATCAAAGCCATTCTTTTTGTAAGCAACTTCTATTCCTAAATTTTAATGGCTTTTAAGCTTAAGTTTAATTTTAATTTCTAAAGTTTCAATCGTGTGATTAACTAAGTTAGTGTAAGAAAGCTCAAGAAGATATTTGTTGCAGAACATATTTGTTGCAGGATTAGACACTGGTTCAATTACAATAGTCTGGGCAATGGCAGAACTCGCAAAAAATGCAAAATTGATGAAGAAAGCTCAAGAAGAAATAAGAAACTATGTGAGAAACAAACAAAAGGTAAGAGAGAAAGACGTCGAAGAGCTTCCATATTTAAAAATGATAGTAAAAGAGACACTAAGACTACATCCACCAGCACCTCTTCTCCTTCCAAGAGAAACCATTTCTCATTTCAAAATTGAAGACTATGATTTTTACCCGAAAACGAAGGTTCAAGTAAATATTTGGGCAATTGGACGAGACCCCACATACTGGACAGACCCAGAAGAGTTCCTTCCAGAGAGATTTGCAGAGAGCTCGGTTGATTACAAAGGACAACATTATGAGTTCTTACCATTTGGGGCTGGTCGGAGAATATGCCCGGGGTTGAATATGGGAGTAAAAACTGTGGAGTTAGCTTTAGCGAATCTTTTGTATCATTTTGATTGGAAGCTTCCGAATGGGATGAAAGAGGAAGAGTTGGATATGGAAGAGAGTTCAGGTTTGAGTCTCACCATCTACAAAAAGTTGCCTCTAAAGCTTGTACCAATTTTATACCATCCATGA

mRNA sequence

ATGTTGGAAAGACCTAGAAGAGTTTCTTCAAGAGAGATTTTGCAGGGAGCTCCATTGATTACAAAGGACTACATTTTGAGTTCTTGCCATTTGGGGGTGGTCGGAGGATATGCTCGGGGTTGGACATGGGAGGTGAAATCTGTGGATTTGGCTTTTGCAAATCTCTTGTACCATTTTGATTGGAAGTTGCCGGAGGGGATGAAGGAGGAGGACTTGGACATGGAAGAGAAATTAGATTATTTTTTACTTTTCAAAAGTTTTCACCCTACAGTGATGACTGACGAGAGATGTGCAAAATTTTGGTTTGAAGGGGATGGATTTCATGAGCATATAAGTCAAGTTCCTGTTGTAGGAGAGCTTTACGGTCCTATTATGAGCCTCAACCTAGGTCGTATAGAAACCATCATCATATCATCGACTGAAACTGCTCGTGCCCTCTTGAAAACTCACGATCTTCAAAGTTGCAACAGACCACAAACTCATGCTATGAAAAAATTCACTTACAATTTTCTTGACCTTGGTTTTTCACCCTACAATGATTACTTGCGAGAGATGCGCAAAATTTCTTCTTGTGGAGCTATTGTTGATCTTAGTGAGAAGTCCATTGCTCTCACTACTGGTGTTATTTTTAGAATTGCATTTGGGAAGCCATTTGAAGGGGATGGATTTCATGAGCTTATAAGTGAAGTTGAAGCCCTGATGGGAAGCTATAGTGCTTCTGAGTTTTTTCCAGTTCCTTTTGTGGGAGAAGTATTTGATTGGTTTAGTGGTCGAGAAGCTAGAATTGAGAAAGTTTTTAATGAGATAAATGCTCTATTTCAAAAGGTGATTGATGAACACCTCTGTCCTGAGAGGCCTAAGCCAGAGCAAGATGATATCATTGATGTGCTTTTGGCTATTGGTAAAAAGCAAGTTCATCCTCACACGGTTGTTATTAACCATGAAAACATCAAAGCCATTCTTTTTAACATATTTGTTGCAGGATTAGACACTGGTTCAATTACAATAGTCTGGGCAATGGCAGAACTCGCAAAAAATGCAAAATTGATGAAGAAAGCTCAAGAAGAAATAAGAAACTATGTGAGAAACAAACAAAAGGTAAGAGAGAAAGACGTCGAAGAGCTTCCATATTTAAAAATGATAGTAAAAGAGACACTAAGACTACATCCACCAGCACCTCTTCTCCTTCCAAGAGAAACCATTTCTCATTTCAAAATTGAAGACTATGATTTTTACCCGAAAACGAAGGTTCAAGTAAATATTTGGGCAATTGGACGAGACCCCACATACTGGACAGACCCAGAAGAGTTCCTTCCAGAGAGATTTGCAGAGAGCTCGGTTGATTACAAAGGACAACATTATGAGTTCTTACCATTTGGGGCTGGTCGGAGAATATGCCCGGGGTTGAATATGGGAGTAAAAACTGTGGAGTTAGCTTTAGCGAATCTTTTGTATCATTTTGATTGGAAGCTTCCGAATGGGATGAAAGAGGAAGAGTTGGATATGGAAGAGAGTTCAGGTTTGAGTCTCACCATCTACAAAAAGTTGCCTCTAAAGCTTGTACCAATTTTATACCATCCATGA

Coding sequence (CDS)

ATGTTGGAAAGACCTAGAAGAGTTTCTTCAAGAGAGATTTTGCAGGGAGCTCCATTGATTACAAAGGACTACATTTTGAGTTCTTGCCATTTGGGGGTGGTCGGAGGATATGCTCGGGGTTGGACATGGGAGGTGAAATCTGTGGATTTGGCTTTTGCAAATCTCTTGTACCATTTTGATTGGAAGTTGCCGGAGGGGATGAAGGAGGAGGACTTGGACATGGAAGAGAAATTAGATTATTTTTTACTTTTCAAAAGTTTTCACCCTACAGTGATGACTGACGAGAGATGTGCAAAATTTTGGTTTGAAGGGGATGGATTTCATGAGCATATAAGTCAAGTTCCTGTTGTAGGAGAGCTTTACGGTCCTATTATGAGCCTCAACCTAGGTCGTATAGAAACCATCATCATATCATCGACTGAAACTGCTCGTGCCCTCTTGAAAACTCACGATCTTCAAAGTTGCAACAGACCACAAACTCATGCTATGAAAAAATTCACTTACAATTTTCTTGACCTTGGTTTTTCACCCTACAATGATTACTTGCGAGAGATGCGCAAAATTTCTTCTTGTGGAGCTATTGTTGATCTTAGTGAGAAGTCCATTGCTCTCACTACTGGTGTTATTTTTAGAATTGCATTTGGGAAGCCATTTGAAGGGGATGGATTTCATGAGCTTATAAGTGAAGTTGAAGCCCTGATGGGAAGCTATAGTGCTTCTGAGTTTTTTCCAGTTCCTTTTGTGGGAGAAGTATTTGATTGGTTTAGTGGTCGAGAAGCTAGAATTGAGAAAGTTTTTAATGAGATAAATGCTCTATTTCAAAAGGTGATTGATGAACACCTCTGTCCTGAGAGGCCTAAGCCAGAGCAAGATGATATCATTGATGTGCTTTTGGCTATTGGTAAAAAGCAAGTTCATCCTCACACGGTTGTTATTAACCATGAAAACATCAAAGCCATTCTTTTTAACATATTTGTTGCAGGATTAGACACTGGTTCAATTACAATAGTCTGGGCAATGGCAGAACTCGCAAAAAATGCAAAATTGATGAAGAAAGCTCAAGAAGAAATAAGAAACTATGTGAGAAACAAACAAAAGGTAAGAGAGAAAGACGTCGAAGAGCTTCCATATTTAAAAATGATAGTAAAAGAGACACTAAGACTACATCCACCAGCACCTCTTCTCCTTCCAAGAGAAACCATTTCTCATTTCAAAATTGAAGACTATGATTTTTACCCGAAAACGAAGGTTCAAGTAAATATTTGGGCAATTGGACGAGACCCCACATACTGGACAGACCCAGAAGAGTTCCTTCCAGAGAGATTTGCAGAGAGCTCGGTTGATTACAAAGGACAACATTATGAGTTCTTACCATTTGGGGCTGGTCGGAGAATATGCCCGGGGTTGAATATGGGAGTAAAAACTGTGGAGTTAGCTTTAGCGAATCTTTTGTATCATTTTGATTGGAAGCTTCCGAATGGGATGAAAGAGGAAGAGTTGGATATGGAAGAGAGTTCAGGTTTGAGTCTCACCATCTACAAAAAGTTGCCTCTAAAGCTTGTACCAATTTTATACCATCCATGA

Protein sequence

MLERPRRVSSREILQGAPLITKDYILSSCHLGVVGGYARGWTWEVKSVDLAFANLLYHFDWKLPEGMKEEDLDMEEKLDYFLLFKSFHPTVMTDERCAKFWFEGDGFHEHISQVPVVGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFSPYNDYLREMRKISSCGAIVDLSEKSIALTTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEKVFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNIFVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKETLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAESSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEESSGLSLTIYKKLPLKLVPILYHP
Homology
BLAST of CmaCh18G007460 vs. ExPASy Swiss-Prot
Match: Q9LTM4 (Cytochrome P450 71B19 OS=Arabidopsis thaliana OX=3702 GN=CYP71B19 PE=1 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 6.6e-112
Identity = 205/446 (45.96%), Postives = 291/446 (65.25%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           + E YGP+M L+ G +   ++SS E A  +L+THDL  C+RP+    +  + +F D+GF+
Sbjct: 57  LAERYGPVMLLHFGFVPITVVSSREAAEEVLRTHDLDCCSRPKLVGTRLLSRDFKDIGFT 116

Query: 177 PYNDYLREMRKIS-------------------SCGAI-------------VDLSEKSIAL 236
           PY +  +  RK +                    C  +             VDLS+    L
Sbjct: 117 PYGNEWKARRKFALRELFCLKKVQSFRHIREEECNFLVKQLSESAVDRSPVDLSKSLFWL 176

Query: 237 TTGVIFRIAFGKPFEGDGF------HELISEVEALMGSYSASEFFPVPFVGEVFDWFSGR 296
           T  ++FR+A G+ F    F       EL+ E E  + S++ S+FFPV  +G + DWFSG+
Sbjct: 177 TASILFRVALGQNFHESDFIDKEKIEELVFEAETALASFTCSDFFPVAGLGWLVDWFSGQ 236

Query: 297 EARIEKVFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIK 356
             R+  VF +++ALFQ VID+HL P R K E +DIID +L +  KQ    ++ +  ++IK
Sbjct: 237 HKRLNDVFYKLDALFQHVIDDHLNPGRSK-EHEDIIDSMLDVIHKQGEDSSLELTIDHIK 296

Query: 357 AILFNIFVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYV-RNKQKVREKDVEELPY 416
             L NIF+AG+DTG+IT++WA+ EL KN KL+KK Q +IR  +  NK+++ E+D+E++PY
Sbjct: 297 GFLANIFLAGIDTGAITMIWAVTELVKNPKLIKKVQGDIREQLGSNKERITEEDIEKVPY 356

Query: 417 LKMIVKETLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEF 476
           LKM++KET RLHP APL+LPRET++H K++ YD  PK ++ VN+ AIGRDP  WT+P+EF
Sbjct: 357 LKMVIKETFRLHPAAPLILPRETMAHIKVQGYDIPPKRRILVNVSAIGRDPKLWTNPKEF 416

Query: 477 LPERFAESSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKE 524
            PERF +S VDY+GQHYE LPFG+GRRICPG+ MG+  VEL L NLLY FDWKLP+GM  
Sbjct: 417 DPERFMDSFVDYRGQHYELLPFGSGRRICPGMPMGIAAVELGLLNLLYFFDWKLPDGMTH 476

BLAST of CmaCh18G007460 vs. ExPASy Swiss-Prot
Match: Q9LTM3 (Cytochrome P450 71B20 OS=Arabidopsis thaliana OX=3702 GN=CYP71B20 PE=2 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 1.2e-110
Identity = 205/446 (45.96%), Postives = 288/446 (64.57%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           + E YGP+M L+ G +   ++SS E A  +L+THDL  C+RP+    +  + NF D+ F+
Sbjct: 57  LAERYGPVMLLHFGFVPVTVVSSREAAEEVLRTHDLDCCSRPKLVGTRLLSRNFKDVCFT 116

Query: 177 PYNDYLREMRKIS-------------------SCGAI-------------VDLSEKSIAL 236
           PY +  +  RK +                    C  +             VDLS+    L
Sbjct: 117 PYGNEWKARRKFALRELFCLKKVQSFRHIREEECNFLVKQLSESAVNRSPVDLSKSLFWL 176

Query: 237 TTGVIFRIAFGKPFEGDGF------HELISEVEALMGSYSASEFFPVPFVGEVFDWFSGR 296
           T  + FR+A G+ F    F       EL+ E E  + S++ S+FFPV  +G + DWFSG+
Sbjct: 177 TASIFFRVALGQNFHESNFIDKEKIEELVFEAETALASFTCSDFFPVAGLGWLVDWFSGQ 236

Query: 297 EARIEKVFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIK 356
             R+  VF +++ALFQ VID+HL P R K E +DIID +L    K+    ++ +  ++IK
Sbjct: 237 HKRLNDVFYKLDALFQHVIDDHLNPGRSK-EHEDIIDSMLDAIHKEGKDSSLELIIDHIK 296

Query: 357 AILFNIFVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYV-RNKQKVREKDVEELPY 416
             L NIF+AG+DTG++T++WAM EL KN KL+KK Q EIR  +  NK ++ E+D++++PY
Sbjct: 297 GFLANIFLAGIDTGALTMIWAMTELVKNPKLIKKVQGEIREQLGSNKARITEEDIDKVPY 356

Query: 417 LKMIVKETLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEF 476
           LKM++KET RLHP APL+LPRET++H K++ YD  PK ++ VN+ AIGRDP  WT+PEEF
Sbjct: 357 LKMVIKETFRLHPAAPLILPRETMAHIKVQGYDIPPKRRILVNVSAIGRDPKLWTNPEEF 416

Query: 477 LPERFAESSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKE 524
            PERF +SSVDY+GQHYE LPFG+GRRICPG+ MG+  VEL L NLLY FDWKLP+GM  
Sbjct: 417 DPERFMDSSVDYRGQHYELLPFGSGRRICPGMPMGIAAVELGLLNLLYFFDWKLPDGMTH 476

BLAST of CmaCh18G007460 vs. ExPASy Swiss-Prot
Match: Q9LTL0 (Cytochrome P450 71B26 OS=Arabidopsis thaliana OX=3702 GN=CYP71B26 PE=2 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.5e-108
Identity = 201/442 (45.48%), Postives = 288/442 (65.16%), Query Frame = 0

Query: 121 YGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFSPYND 180
           YGP+M L LG++ T+I+SS+ETA+  L+ +DL  C+RP     ++ +YN LD+  SPYN+
Sbjct: 62  YGPVMLLKLGKVPTLILSSSETAKQALRDYDLHCCSRPSLAGGRELSYNNLDMSSSPYNE 121

Query: 181 YLREMRKI--------------------------------SSCGAIVDLSEKSIALTTGV 240
           Y +E+RK+                                SS    V+LS+  +ALTT V
Sbjct: 122 YWKELRKLCSQELFSANKIQSIQPIKDEEVKKVIDSIAESSSLKNPVNLSKTFLALTTSV 181

Query: 241 IFRIAFGKPFEG-----DGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIE 300
           + + AFG  FEG     D F++L+ +   ++GS+SAS+F  +P+VG + D F+G +   +
Sbjct: 182 VCKAAFGVSFEGSVLNSDRFNKLVRDTFEMLGSFSASDF--IPYVGWIIDKFNGLQGWRK 241

Query: 301 KVFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFN 360
           K F +++A ++++ D H   E  +   +D++DVLL + K+++      +   +IKAIL N
Sbjct: 242 KSFRDLDAFYEQIFDLH--KEEKEVGSEDLVDVLLRLEKEEIVVGNGKLTRNHIKAILMN 301

Query: 361 IFVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVK 420
           I + G+DT +IT+ WAMAELAKN ++MKK Q EIRN ++NK+++   D ++L YLKM++K
Sbjct: 302 ILLGGIDTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNKERISFDDTDKLEYLKMVIK 361

Query: 421 ETLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFA 480
           ET RLHPP PLLLPR+ I+ F+I  Y    KT++ VN+WAIGRDP  W DPE FLPERF 
Sbjct: 362 ETWRLHPPTPLLLPRDVITEFEINGYTIPAKTRLHVNVWAIGRDPDTWKDPEMFLPERFN 421

Query: 481 ESSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDME 526
           +S++D KGQ++E L FG+GRRICPGL MG   VE  LAN+LYHFDWKLP GM  E++DME
Sbjct: 422 DSNIDAKGQNFELLSFGSGRRICPGLYMGTTMVEFGLANMLYHFDWKLPEGMVVEDIDME 481

BLAST of CmaCh18G007460 vs. ExPASy Swiss-Prot
Match: O65788 (Cytochrome P450 71B2 OS=Arabidopsis thaliana OX=3702 GN=CYP71B2 PE=2 SV=2)

HSP 1 Score: 394.0 bits (1011), Expect = 2.6e-108
Identity = 199/443 (44.92%), Postives = 291/443 (65.69%), Query Frame = 0

Query: 121 YGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFSPYND 180
           YGP++ L LG +  ++ISS+E A A+LKT+DL+ C+RP+T    K +Y F D+ F+PY +
Sbjct: 62  YGPLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGE 121

Query: 181 YLREMRKIS----------------------------SCGAI----VDLSEKSIALTTGV 240
           Y RE+RK++                            S  A+    VDLS+   +LT  +
Sbjct: 122 YWREVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASI 181

Query: 241 IFRIAFGKPFEGDGF-------HELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREAR 300
           I R+A G+ F   GF        EL++E    +G+++ S+FFP   +G   DW   R  +
Sbjct: 182 ICRVALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFP-GGLGRFVDWLFQRHKK 241

Query: 301 IEKVFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAIL 360
           I KVF E++A +Q VID+HL PE  K    DI+ ++L +  KQ    +  +N +N+KAI+
Sbjct: 242 INKVFKELDAFYQHVIDDHLKPEGRK--NQDIVTLILDMIDKQEDSDSFKLNMDNLKAIV 301

Query: 361 FNIFVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYV-RNKQKVREKDVEELPYLKM 420
            ++F+AG+DT ++T++WAM EL +N ++MKKAQE IR  +   K+++ E+D+ ++ YL  
Sbjct: 302 MDVFLAGIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEYLNH 361

Query: 421 IVKETLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPE 480
           I+KET RLHP  P ++PRET+SH KI+ YD  PKT++Q+N+W IGRDP  W DPEEF PE
Sbjct: 362 ILKETFRLHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEFNPE 421

Query: 481 RFAESSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEEL 524
           RFA SSVD++GQH++ LPFG+GRRICPG+ M + +VELAL NLLY+FDW +P+G K E++
Sbjct: 422 RFANSSVDFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTKGEDI 481

BLAST of CmaCh18G007460 vs. ExPASy Swiss-Prot
Match: Q9LVD2 (Cytochrome P450 71B10 OS=Arabidopsis thaliana OX=3702 GN=CYP71B10 PE=3 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.1e-106
Identity = 201/443 (45.37%), Postives = 281/443 (63.43%), Query Frame = 0

Query: 121 YGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFSPYND 180
           YGP+M L LGR+ T+I+S+ ETA+ +LK +DL  C+RP     +K +YN+LD+ FS ++D
Sbjct: 61  YGPVMLLKLGRVPTVIVSTPETAKQVLKDYDLHCCSRPSLEGTRKLSYNYLDIAFSRFDD 120

Query: 181 YLREMRKI--------------------------------SSCGAIVDLSEKSIALTTGV 240
           Y +E+RK+                                +S   +V+LS+  ++L   V
Sbjct: 121 YWKELRKLCVEELFCNKRINSIQPIKEAEMEKLIDSIAESASQKTLVNLSDTFLSLNVNV 180

Query: 241 IFRIAFGKPFEG-----DGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIE 300
           I +  FG  F+G     D F +L+ E   ++GS+SAS+FF  P+VG + DWF+G  AR E
Sbjct: 181 ICKAVFGVNFQGTVLNNDKFQDLVHEALEMLGSFSASDFF--PYVGWIVDWFTGLHARRE 240

Query: 301 KVFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFN 360
           +   +++A ++++ID HL   R + E DD +D+LL + K++       +   +IKAIL N
Sbjct: 241 RSVRDLDAFYEQMIDLHLQKNREESE-DDFVDLLLRLEKEEAVLGYGKLTRNHIKAILMN 300

Query: 361 IFVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEE---LPYLKM 420
           I + G++T +IT+ WAMAEL +N ++MKK Q EIR  +    K R   ++E   L YL M
Sbjct: 301 ILLGGINTSAITMTWAMAELIRNPRVMKKVQSEIRAQIGKNNKTRIISLDEINHLSYLNM 360

Query: 421 IVKETLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPE 480
           ++KET RLHP APLL+PRE IS FKI  Y   PKT++ VN+WAIGRDP  W DPEEFLPE
Sbjct: 361 VIKETCRLHPVAPLLVPREVISEFKINGYTIQPKTRLHVNVWAIGRDPEIWKDPEEFLPE 420

Query: 481 RFAESSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEEL 524
           RF +  +D KGQ YE LPFG+GRRICP + MG+ TVE  LANLLYHFDWKLP G+  E++
Sbjct: 421 RFMDCDIDVKGQDYELLPFGSGRRICPAVYMGITTVEFGLANLLYHFDWKLPEGVAVEDI 480

BLAST of CmaCh18G007460 vs. ExPASy TrEMBL
Match: A0A6J1HQM2 (cytochrome P450 71B19-like OS=Cucurbita maxima OX=3661 GN=LOC111466486 PE=3 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 1.9e-231
Identity = 407/443 (91.87%), Postives = 410/443 (92.55%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           + +LYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS
Sbjct: 63  LSQLYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 122

Query: 177 PYNDYLREMRKI--------------------------------SSCGAIVDLSEKSIAL 236
           PYNDYLREMRKI                                +SCGAIVDLSEKSIAL
Sbjct: 123 PYNDYLREMRKICMLEFFSMKRVLSYKPIREQEVCLLIESISQSASCGAIVDLSEKSIAL 182

Query: 237 TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK 296
           TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK
Sbjct: 183 TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK 242

Query: 297 VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI 356
           VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI
Sbjct: 243 VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI 302

Query: 357 FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE 416
           FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE
Sbjct: 303 FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE 362

Query: 417 TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE 476
           TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE
Sbjct: 363 TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE 422

Query: 477 SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE 528
           SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE
Sbjct: 423 SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE 482

BLAST of CmaCh18G007460 vs. ExPASy TrEMBL
Match: A0A6J1FZI8 (cytochrome P450 71B34-like OS=Cucurbita moschata OX=3662 GN=LOC111449355 PE=3 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 1.7e-208
Identity = 364/443 (82.17%), Postives = 392/443 (88.49%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           + +LYGPIMSL+LGRIETIIISS ET RALLKTHDLQSCNRPQT A+KKF+YNFLD+ FS
Sbjct: 63  LSQLYGPIMSLSLGRIETIIISSAETTRALLKTHDLQSCNRPQTQAVKKFSYNFLDIAFS 122

Query: 177 PYNDYLREMRKI--------------------------------SSCGAIVDLSEKSIAL 236
           PY+DY RE+RKI                                +SCGA+VDLSEKSIAL
Sbjct: 123 PYSDYWREIRKICMLEFFNMKRVLSYEPIREQEVGLLIESISQSASCGAVVDLSEKSIAL 182

Query: 237 TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK 296
           T GVIFRIAFGKPFEG+GFHELISE+EAL+GSYSASEFFPVPFVGEVFDWFSGRE ++E+
Sbjct: 183 TAGVIFRIAFGKPFEGNGFHELISEIEALLGSYSASEFFPVPFVGEVFDWFSGRETKLER 242

Query: 297 VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI 356
           VFNE+NALFQ+VIDEHLCPER KPEQDDIIDVLLAI KKQV P TVVI HENIKAILF+I
Sbjct: 243 VFNEMNALFQEVIDEHLCPERLKPEQDDIIDVLLAISKKQVEPCTVVITHENIKAILFDI 302

Query: 357 FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE 416
           FVAGLDTGSITIVWAMAELAKN+KLMKKAQEEIRNYV NK+KV EKDVEELPYLKMIVKE
Sbjct: 303 FVAGLDTGSITIVWAMAELAKNSKLMKKAQEEIRNYVGNKRKVTEKDVEELPYLKMIVKE 362

Query: 417 TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE 476
           TLRLHPPAPLLLPRETISHFKIEDY+FYPKT VQVN+WAIGRDPT W DPEEFLPERFAE
Sbjct: 363 TLRLHPPAPLLLPRETISHFKIEDYNFYPKTMVQVNVWAIGRDPTCWKDPEEFLPERFAE 422

Query: 477 SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE 528
           SS+DYKGQH+EFLPFG GRRICPGLNMGVKTVELALANLLYH+DWKLPNGMKEE+LDMEE
Sbjct: 423 SSIDYKGQHFEFLPFGVGRRICPGLNMGVKTVELALANLLYHYDWKLPNGMKEEDLDMEE 482

BLAST of CmaCh18G007460 vs. ExPASy TrEMBL
Match: A0A6J1HTM0 (cytochrome P450 71B34-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466484 PE=3 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 1.1e-207
Identity = 362/443 (81.72%), Postives = 390/443 (88.04%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           + +LYGPIMSL+LGRIE IIISS ETARALLK HDLQSCNRPQTHA+KKF+YNFLD+ FS
Sbjct: 76  LSQLYGPIMSLSLGRIEIIIISSVETARALLKIHDLQSCNRPQTHAIKKFSYNFLDIAFS 135

Query: 177 PYNDYLREMRKI--------------------------------SSCGAIVDLSEKSIAL 236
           PY+DY RE+RKI                                +SCGA+VDLSEKSIAL
Sbjct: 136 PYSDYWREIRKICMLEFFSMKRVLSYKPIREQEVGLFIESISQSASCGAVVDLSEKSIAL 195

Query: 237 TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK 296
           T GVIFRIAFGK FEGDGFHELISEVEAL+GSYSASEFFPVPFVGEVFDWFSGRE R+E+
Sbjct: 196 TAGVIFRIAFGKQFEGDGFHELISEVEALLGSYSASEFFPVPFVGEVFDWFSGRETRLER 255

Query: 297 VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI 356
           VFNE+N LFQ+VIDEHLCP+RPKPEQDDIIDVLLAI K QV P +VVI HENIKAILF+I
Sbjct: 256 VFNEMNGLFQEVIDEHLCPQRPKPEQDDIIDVLLAISKNQVEPCSVVITHENIKAILFDI 315

Query: 357 FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE 416
           F+AGLDT SITIVWAMAELAKN+KLMKKAQEEIRNYV NK+KV EKDVEELPYLKMIVKE
Sbjct: 316 FLAGLDTSSITIVWAMAELAKNSKLMKKAQEEIRNYVGNKRKVTEKDVEELPYLKMIVKE 375

Query: 417 TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE 476
           TLRLHPPAPLLLPRETISHFKIEDY+FYPKT VQVN+WAIGRDPTYW DPEEFLPERFAE
Sbjct: 376 TLRLHPPAPLLLPRETISHFKIEDYNFYPKTMVQVNVWAIGRDPTYWKDPEEFLPERFAE 435

Query: 477 SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE 528
           SS+DYKGQH+E LPFGAGRRICPGLNMGVKTVELALANLLYH+DWKLPNGMKEE+LDMEE
Sbjct: 436 SSIDYKGQHFELLPFGAGRRICPGLNMGVKTVELALANLLYHYDWKLPNGMKEEDLDMEE 495

BLAST of CmaCh18G007460 vs. ExPASy TrEMBL
Match: A0A6J1HTN1 (cytochrome P450 71B34-like OS=Cucurbita maxima OX=3661 GN=LOC111466493 PE=3 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 5.3e-205
Identity = 360/443 (81.26%), Postives = 388/443 (87.58%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           + +LYGPIMSL+LGRIE IIISS ETARALLK HDLQSCNRPQTHA+KKF+YNFLD+ FS
Sbjct: 60  LSQLYGPIMSLSLGRIEIIIISSVETARALLKNHDLQSCNRPQTHAIKKFSYNFLDIAFS 119

Query: 177 PYNDYLREMRKI--------------------------------SSCGAIVDLSEKSIAL 236
           P +DY RE+RKI                                +SCGA+VDLSEKSIAL
Sbjct: 120 PCSDYWREIRKICMLEFFSMKRVLSYEPIREQEVGLFIESISQSASCGAVVDLSEKSIAL 179

Query: 237 TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK 296
           TTGVIFRIAFGK FEGDGFHELISEVEAL+GSYSASEFFPVPFVGEVFDWFSGRE R+E+
Sbjct: 180 TTGVIFRIAFGKQFEGDGFHELISEVEALLGSYSASEFFPVPFVGEVFDWFSGRETRLER 239

Query: 297 VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI 356
           VFNE+N LFQ+VIDEHLCP+RPKPEQDDIIDVLLAI K QV P +VVI HENIKAILF+I
Sbjct: 240 VFNEMNGLFQEVIDEHLCPQRPKPEQDDIIDVLLAISKNQVEPCSVVITHENIKAILFDI 299

Query: 357 FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE 416
           F+AGLDT SITIVWAMAELAKN+KLMKKAQEEIRNYV NK+KV EKDVEELPYLKMIVKE
Sbjct: 300 FLAGLDTSSITIVWAMAELAKNSKLMKKAQEEIRNYVGNKRKVTEKDVEELPYLKMIVKE 359

Query: 417 TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE 476
           TLRLHPPAP LLPRETISHFKIEDY+FYPKT VQVN+WAIGRDPTYW DPEEFLPERFAE
Sbjct: 360 TLRLHPPAP-LLPRETISHFKIEDYNFYPKTMVQVNVWAIGRDPTYWKDPEEFLPERFAE 419

Query: 477 SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE 528
           SS+DYKGQH+E LPFGAGRRICPGLNMGVKTVE ALANLLYH+DWKLPNGMKEE+LDMEE
Sbjct: 420 SSIDYKGQHFELLPFGAGRRICPGLNMGVKTVEFALANLLYHYDWKLPNGMKEEDLDMEE 479

BLAST of CmaCh18G007460 vs. ExPASy TrEMBL
Match: A0A6J1G0A5 (cytochrome P450 71B19-like OS=Cucurbita moschata OX=3662 GN=LOC111449539 PE=3 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 1.6e-201
Identity = 356/443 (80.36%), Postives = 385/443 (86.91%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           +  +YGPIMSLNLGRIETIIISS ETARALLKTHDLQSCNRPQTHA+KKF+YNFLD+GFS
Sbjct: 62  LSRIYGPIMSLNLGRIETIIISSAETARALLKTHDLQSCNRPQTHAIKKFSYNFLDIGFS 121

Query: 177 PYNDYLREMRKI--------------------------------SSCGAIVDLSEKSIAL 236
           PYN Y RE+RKI                                +S GAIVD+SEKSIAL
Sbjct: 122 PYNSYWREIRKICMLEFFSMKRVLSYEPIREQEVGLIIESISQSASRGAIVDISEKSIAL 181

Query: 237 TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK 296
           TTGVIFRIAFGK FEGDGF+ELISEVEA +GSYSASE FP PFVGEVFDWFSGREAR+E+
Sbjct: 182 TTGVIFRIAFGKRFEGDGFNELISEVEAQIGSYSASEIFPAPFVGEVFDWFSGREARLER 241

Query: 297 VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI 356
           VFNE+NALFQ+VIDEHLCPERPKPEQDDIIDVLLAI KKQV   TVVI  ENIKAILFNI
Sbjct: 242 VFNEMNALFQEVIDEHLCPERPKPEQDDIIDVLLAISKKQVGSCTVVITQENIKAILFNI 301

Query: 357 FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE 416
           FVAGLDTGSITIVWAM ELAKN+K+MKKAQEEIRNYV NK+KV E+D+EELPYLKMIVKE
Sbjct: 302 FVAGLDTGSITIVWAMTELAKNSKVMKKAQEEIRNYVGNKRKVTERDIEELPYLKMIVKE 361

Query: 417 TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE 476
           TLRLHPPAP LLPRETISHFKI DYDFYPK+ VQVN+WAIGRDPT WTDPEEFLPERF E
Sbjct: 362 TLRLHPPAP-LLPRETISHFKIGDYDFYPKSMVQVNVWAIGRDPTCWTDPEEFLPERFTE 421

Query: 477 SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE 528
           SS+DY GQH+EFLPFGAGRR+CPGLNMGVKTVELALANLLYHFDWKLPNGMK+E+LDMEE
Sbjct: 422 SSIDYNGQHFEFLPFGAGRRMCPGLNMGVKTVELALANLLYHFDWKLPNGMKKEDLDMEE 481

BLAST of CmaCh18G007460 vs. NCBI nr
Match: XP_022966921.1 (cytochrome P450 71B19-like [Cucurbita maxima])

HSP 1 Score: 811.6 bits (2095), Expect = 4.0e-231
Identity = 407/443 (91.87%), Postives = 410/443 (92.55%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           + +LYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS
Sbjct: 63  LSQLYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 122

Query: 177 PYNDYLREMRKI--------------------------------SSCGAIVDLSEKSIAL 236
           PYNDYLREMRKI                                +SCGAIVDLSEKSIAL
Sbjct: 123 PYNDYLREMRKICMLEFFSMKRVLSYKPIREQEVCLLIESISQSASCGAIVDLSEKSIAL 182

Query: 237 TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK 296
           TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK
Sbjct: 183 TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK 242

Query: 297 VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI 356
           VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI
Sbjct: 243 VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI 302

Query: 357 FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE 416
           FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE
Sbjct: 303 FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE 362

Query: 417 TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE 476
           TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE
Sbjct: 363 TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE 422

Query: 477 SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE 528
           SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE
Sbjct: 423 SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE 482

BLAST of CmaCh18G007460 vs. NCBI nr
Match: XP_023542050.1 (cytochrome P450 71B19-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 756.1 bits (1951), Expect = 2.0e-214
Identity = 377/443 (85.10%), Postives = 397/443 (89.62%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           + +LYGPIMSLNLGRIETIIISS ETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS
Sbjct: 63  LSQLYGPIMSLNLGRIETIIISSAETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 122

Query: 177 PYNDYLREMRKI--------------------------------SSCGAIVDLSEKSIAL 236
           PY+DY REMRKI                                +SCGA VDLSEKSIAL
Sbjct: 123 PYSDYWREMRKICMLEFFSMKRVLSYEPIREQEVGLLIESILQSASCGATVDLSEKSIAL 182

Query: 237 TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK 296
           TTGVIFRIAFGKPFEG+GFHELISEVEALMGSYSASEFFPVPFVGE+FDWF+GR+AR+EK
Sbjct: 183 TTGVIFRIAFGKPFEGNGFHELISEVEALMGSYSASEFFPVPFVGEIFDWFNGRKARLEK 242

Query: 297 VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI 356
           VFNEINALFQ+VI+EHLCPERPKP QDDIIDVLLAI KKQV P T+VINHENIKAILFNI
Sbjct: 243 VFNEINALFQEVINEHLCPERPKPVQDDIIDVLLAISKKQVDPCTIVINHENIKAILFNI 302

Query: 357 FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE 416
           FVAGLDTGSITIVWAMAELAKN+KLMKKAQEEIRNYV NK KVRE+DVEELPYLKMIVKE
Sbjct: 303 FVAGLDTGSITIVWAMAELAKNSKLMKKAQEEIRNYVGNKGKVRERDVEELPYLKMIVKE 362

Query: 417 TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE 476
           TLRLHPPAPLLLPRETISHFKIE+YDFYPKT VQVNIWAIGRDPT WTDPEEFLPERFAE
Sbjct: 363 TLRLHPPAPLLLPRETISHFKIENYDFYPKTMVQVNIWAIGRDPTCWTDPEEFLPERFAE 422

Query: 477 SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE 528
           SS+DYKGQH+EFLPFGAGRRICPG+NMGVKTVELAL NLLYHFDWKL NGMKEE+LDMEE
Sbjct: 423 SSIDYKGQHFEFLPFGAGRRICPGINMGVKTVELALTNLLYHFDWKLSNGMKEEDLDMEE 482

BLAST of CmaCh18G007460 vs. NCBI nr
Match: XP_022944978.1 (cytochrome P450 71B34-like [Cucurbita moschata])

HSP 1 Score: 735.3 bits (1897), Expect = 3.6e-208
Identity = 364/443 (82.17%), Postives = 392/443 (88.49%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           + +LYGPIMSL+LGRIETIIISS ET RALLKTHDLQSCNRPQT A+KKF+YNFLD+ FS
Sbjct: 63  LSQLYGPIMSLSLGRIETIIISSAETTRALLKTHDLQSCNRPQTQAVKKFSYNFLDIAFS 122

Query: 177 PYNDYLREMRKI--------------------------------SSCGAIVDLSEKSIAL 236
           PY+DY RE+RKI                                +SCGA+VDLSEKSIAL
Sbjct: 123 PYSDYWREIRKICMLEFFNMKRVLSYEPIREQEVGLLIESISQSASCGAVVDLSEKSIAL 182

Query: 237 TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK 296
           T GVIFRIAFGKPFEG+GFHELISE+EAL+GSYSASEFFPVPFVGEVFDWFSGRE ++E+
Sbjct: 183 TAGVIFRIAFGKPFEGNGFHELISEIEALLGSYSASEFFPVPFVGEVFDWFSGRETKLER 242

Query: 297 VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI 356
           VFNE+NALFQ+VIDEHLCPER KPEQDDIIDVLLAI KKQV P TVVI HENIKAILF+I
Sbjct: 243 VFNEMNALFQEVIDEHLCPERLKPEQDDIIDVLLAISKKQVEPCTVVITHENIKAILFDI 302

Query: 357 FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE 416
           FVAGLDTGSITIVWAMAELAKN+KLMKKAQEEIRNYV NK+KV EKDVEELPYLKMIVKE
Sbjct: 303 FVAGLDTGSITIVWAMAELAKNSKLMKKAQEEIRNYVGNKRKVTEKDVEELPYLKMIVKE 362

Query: 417 TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE 476
           TLRLHPPAPLLLPRETISHFKIEDY+FYPKT VQVN+WAIGRDPT W DPEEFLPERFAE
Sbjct: 363 TLRLHPPAPLLLPRETISHFKIEDYNFYPKTMVQVNVWAIGRDPTCWKDPEEFLPERFAE 422

Query: 477 SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE 528
           SS+DYKGQH+EFLPFG GRRICPGLNMGVKTVELALANLLYH+DWKLPNGMKEE+LDMEE
Sbjct: 423 SSIDYKGQHFEFLPFGVGRRICPGLNMGVKTVELALANLLYHYDWKLPNGMKEEDLDMEE 482

BLAST of CmaCh18G007460 vs. NCBI nr
Match: XP_022966918.1 (cytochrome P450 71B34-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 732.6 bits (1890), Expect = 2.3e-207
Identity = 362/443 (81.72%), Postives = 390/443 (88.04%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           + +LYGPIMSL+LGRIE IIISS ETARALLK HDLQSCNRPQTHA+KKF+YNFLD+ FS
Sbjct: 76  LSQLYGPIMSLSLGRIEIIIISSVETARALLKIHDLQSCNRPQTHAIKKFSYNFLDIAFS 135

Query: 177 PYNDYLREMRKI--------------------------------SSCGAIVDLSEKSIAL 236
           PY+DY RE+RKI                                +SCGA+VDLSEKSIAL
Sbjct: 136 PYSDYWREIRKICMLEFFSMKRVLSYKPIREQEVGLFIESISQSASCGAVVDLSEKSIAL 195

Query: 237 TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK 296
           T GVIFRIAFGK FEGDGFHELISEVEAL+GSYSASEFFPVPFVGEVFDWFSGRE R+E+
Sbjct: 196 TAGVIFRIAFGKQFEGDGFHELISEVEALLGSYSASEFFPVPFVGEVFDWFSGRETRLER 255

Query: 297 VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI 356
           VFNE+N LFQ+VIDEHLCP+RPKPEQDDIIDVLLAI K QV P +VVI HENIKAILF+I
Sbjct: 256 VFNEMNGLFQEVIDEHLCPQRPKPEQDDIIDVLLAISKNQVEPCSVVITHENIKAILFDI 315

Query: 357 FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE 416
           F+AGLDT SITIVWAMAELAKN+KLMKKAQEEIRNYV NK+KV EKDVEELPYLKMIVKE
Sbjct: 316 FLAGLDTSSITIVWAMAELAKNSKLMKKAQEEIRNYVGNKRKVTEKDVEELPYLKMIVKE 375

Query: 417 TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE 476
           TLRLHPPAPLLLPRETISHFKIEDY+FYPKT VQVN+WAIGRDPTYW DPEEFLPERFAE
Sbjct: 376 TLRLHPPAPLLLPRETISHFKIEDYNFYPKTMVQVNVWAIGRDPTYWKDPEEFLPERFAE 435

Query: 477 SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE 528
           SS+DYKGQH+E LPFGAGRRICPGLNMGVKTVELALANLLYH+DWKLPNGMKEE+LDMEE
Sbjct: 436 SSIDYKGQHFELLPFGAGRRICPGLNMGVKTVELALANLLYHYDWKLPNGMKEEDLDMEE 495

BLAST of CmaCh18G007460 vs. NCBI nr
Match: XP_023542080.1 (cytochrome P450 71B19-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 727.6 bits (1877), Expect = 7.5e-206
Identity = 357/443 (80.59%), Postives = 390/443 (88.04%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           + +LYGPIMSL+LGRIETIIISS ETAR LLK HDLQSCNRPQTHA+KKF+YNFLD+ FS
Sbjct: 85  LSQLYGPIMSLSLGRIETIIISSAETARTLLKIHDLQSCNRPQTHAIKKFSYNFLDIAFS 144

Query: 177 PYNDYLREMRKI--------------------------------SSCGAIVDLSEKSIAL 236
           PY+DY RE+RK+                                +SCGA+VDLSEKSIAL
Sbjct: 145 PYSDYWREIRKVCMLEFFNMKRVLSYEPIREQEVGLLIESISQSASCGAVVDLSEKSIAL 204

Query: 237 TTGVIFRIAFGKPFEGDGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIEK 296
           T GVIFRIAFGK FEGDGFHELISE+EAL+GSYSASEFFP+PFVGEVFDWFSGR+ R+ +
Sbjct: 205 TAGVIFRIAFGKRFEGDGFHELISEIEALLGSYSASEFFPIPFVGEVFDWFSGRKTRLGR 264

Query: 297 VFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFNI 356
           VFNE+NALFQ+VIDEHLCPERPKPEQDDIIDVLLAI KKQV P TVVI HENIKAILF+I
Sbjct: 265 VFNEMNALFQEVIDEHLCPERPKPEQDDIIDVLLAISKKQVEPCTVVITHENIKAILFDI 324

Query: 357 FVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVKE 416
           FVAGLDTGSITIVWAMAELAKN+KLMKKAQEEIRNYV NK+KV E+D+EEL YLKMIVKE
Sbjct: 325 FVAGLDTGSITIVWAMAELAKNSKLMKKAQEEIRNYVGNKRKVTERDIEELSYLKMIVKE 384

Query: 417 TLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFAE 476
           TLRLHPPAPLLLPRETISHFKIE+YDFYPKT VQVN+WAIGRDPT WTDPEEF+PERFAE
Sbjct: 385 TLRLHPPAPLLLPRETISHFKIEEYDFYPKTMVQVNVWAIGRDPTCWTDPEEFIPERFAE 444

Query: 477 SSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDMEE 528
           SS+DYKGQ++EFLPFG GRRICPGLN+GVKTVEL LANLLYHFDWKLPNGMKEE+LDMEE
Sbjct: 445 SSIDYKGQYFEFLPFGTGRRICPGLNIGVKTVELTLANLLYHFDWKLPNGMKEEDLDMEE 504

BLAST of CmaCh18G007460 vs. TAIR 10
Match: AT3G26170.1 (cytochrome P450, family 71, subfamily B, polypeptide 19 )

HSP 1 Score: 406.0 bits (1042), Expect = 4.7e-113
Identity = 205/446 (45.96%), Postives = 291/446 (65.25%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           + E YGP+M L+ G +   ++SS E A  +L+THDL  C+RP+    +  + +F D+GF+
Sbjct: 57  LAERYGPVMLLHFGFVPITVVSSREAAEEVLRTHDLDCCSRPKLVGTRLLSRDFKDIGFT 116

Query: 177 PYNDYLREMRKIS-------------------SCGAI-------------VDLSEKSIAL 236
           PY +  +  RK +                    C  +             VDLS+    L
Sbjct: 117 PYGNEWKARRKFALRELFCLKKVQSFRHIREEECNFLVKQLSESAVDRSPVDLSKSLFWL 176

Query: 237 TTGVIFRIAFGKPFEGDGF------HELISEVEALMGSYSASEFFPVPFVGEVFDWFSGR 296
           T  ++FR+A G+ F    F       EL+ E E  + S++ S+FFPV  +G + DWFSG+
Sbjct: 177 TASILFRVALGQNFHESDFIDKEKIEELVFEAETALASFTCSDFFPVAGLGWLVDWFSGQ 236

Query: 297 EARIEKVFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIK 356
             R+  VF +++ALFQ VID+HL P R K E +DIID +L +  KQ    ++ +  ++IK
Sbjct: 237 HKRLNDVFYKLDALFQHVIDDHLNPGRSK-EHEDIIDSMLDVIHKQGEDSSLELTIDHIK 296

Query: 357 AILFNIFVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYV-RNKQKVREKDVEELPY 416
             L NIF+AG+DTG+IT++WA+ EL KN KL+KK Q +IR  +  NK+++ E+D+E++PY
Sbjct: 297 GFLANIFLAGIDTGAITMIWAVTELVKNPKLIKKVQGDIREQLGSNKERITEEDIEKVPY 356

Query: 417 LKMIVKETLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEF 476
           LKM++KET RLHP APL+LPRET++H K++ YD  PK ++ VN+ AIGRDP  WT+P+EF
Sbjct: 357 LKMVIKETFRLHPAAPLILPRETMAHIKVQGYDIPPKRRILVNVSAIGRDPKLWTNPKEF 416

Query: 477 LPERFAESSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKE 524
            PERF +S VDY+GQHYE LPFG+GRRICPG+ MG+  VEL L NLLY FDWKLP+GM  
Sbjct: 417 DPERFMDSFVDYRGQHYELLPFGSGRRICPGMPMGIAAVELGLLNLLYFFDWKLPDGMTH 476

BLAST of CmaCh18G007460 vs. TAIR 10
Match: AT3G26180.1 (cytochrome P450, family 71, subfamily B, polypeptide 20 )

HSP 1 Score: 401.7 bits (1031), Expect = 8.8e-112
Identity = 205/446 (45.96%), Postives = 288/446 (64.57%), Query Frame = 0

Query: 117 VGELYGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFS 176
           + E YGP+M L+ G +   ++SS E A  +L+THDL  C+RP+    +  + NF D+ F+
Sbjct: 57  LAERYGPVMLLHFGFVPVTVVSSREAAEEVLRTHDLDCCSRPKLVGTRLLSRNFKDVCFT 116

Query: 177 PYNDYLREMRKIS-------------------SCGAI-------------VDLSEKSIAL 236
           PY +  +  RK +                    C  +             VDLS+    L
Sbjct: 117 PYGNEWKARRKFALRELFCLKKVQSFRHIREEECNFLVKQLSESAVNRSPVDLSKSLFWL 176

Query: 237 TTGVIFRIAFGKPFEGDGF------HELISEVEALMGSYSASEFFPVPFVGEVFDWFSGR 296
           T  + FR+A G+ F    F       EL+ E E  + S++ S+FFPV  +G + DWFSG+
Sbjct: 177 TASIFFRVALGQNFHESNFIDKEKIEELVFEAETALASFTCSDFFPVAGLGWLVDWFSGQ 236

Query: 297 EARIEKVFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIK 356
             R+  VF +++ALFQ VID+HL P R K E +DIID +L    K+    ++ +  ++IK
Sbjct: 237 HKRLNDVFYKLDALFQHVIDDHLNPGRSK-EHEDIIDSMLDAIHKEGKDSSLELIIDHIK 296

Query: 357 AILFNIFVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYV-RNKQKVREKDVEELPY 416
             L NIF+AG+DTG++T++WAM EL KN KL+KK Q EIR  +  NK ++ E+D++++PY
Sbjct: 297 GFLANIFLAGIDTGALTMIWAMTELVKNPKLIKKVQGEIREQLGSNKARITEEDIDKVPY 356

Query: 417 LKMIVKETLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEF 476
           LKM++KET RLHP APL+LPRET++H K++ YD  PK ++ VN+ AIGRDP  WT+PEEF
Sbjct: 357 LKMVIKETFRLHPAAPLILPRETMAHIKVQGYDIPPKRRILVNVSAIGRDPKLWTNPEEF 416

Query: 477 LPERFAESSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKE 524
            PERF +SSVDY+GQHYE LPFG+GRRICPG+ MG+  VEL L NLLY FDWKLP+GM  
Sbjct: 417 DPERFMDSSVDYRGQHYELLPFGSGRRICPGMPMGIAAVELGLLNLLYFFDWKLPDGMTH 476

BLAST of CmaCh18G007460 vs. TAIR 10
Match: AT3G26290.1 (cytochrome P450, family 71, subfamily B, polypeptide 26 )

HSP 1 Score: 394.8 bits (1013), Expect = 1.1e-109
Identity = 201/442 (45.48%), Postives = 288/442 (65.16%), Query Frame = 0

Query: 121 YGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFSPYND 180
           YGP+M L LG++ T+I+SS+ETA+  L+ +DL  C+RP     ++ +YN LD+  SPYN+
Sbjct: 62  YGPVMLLKLGKVPTLILSSSETAKQALRDYDLHCCSRPSLAGGRELSYNNLDMSSSPYNE 121

Query: 181 YLREMRKI--------------------------------SSCGAIVDLSEKSIALTTGV 240
           Y +E+RK+                                SS    V+LS+  +ALTT V
Sbjct: 122 YWKELRKLCSQELFSANKIQSIQPIKDEEVKKVIDSIAESSSLKNPVNLSKTFLALTTSV 181

Query: 241 IFRIAFGKPFEG-----DGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIE 300
           + + AFG  FEG     D F++L+ +   ++GS+SAS+F  +P+VG + D F+G +   +
Sbjct: 182 VCKAAFGVSFEGSVLNSDRFNKLVRDTFEMLGSFSASDF--IPYVGWIIDKFNGLQGWRK 241

Query: 301 KVFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFN 360
           K F +++A ++++ D H   E  +   +D++DVLL + K+++      +   +IKAIL N
Sbjct: 242 KSFRDLDAFYEQIFDLH--KEEKEVGSEDLVDVLLRLEKEEIVVGNGKLTRNHIKAILMN 301

Query: 361 IFVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEELPYLKMIVK 420
           I + G+DT +IT+ WAMAELAKN ++MKK Q EIRN ++NK+++   D ++L YLKM++K
Sbjct: 302 ILLGGIDTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNKERISFDDTDKLEYLKMVIK 361

Query: 421 ETLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPERFA 480
           ET RLHPP PLLLPR+ I+ F+I  Y    KT++ VN+WAIGRDP  W DPE FLPERF 
Sbjct: 362 ETWRLHPPTPLLLPRDVITEFEINGYTIPAKTRLHVNVWAIGRDPDTWKDPEMFLPERFN 421

Query: 481 ESSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEELDME 526
           +S++D KGQ++E L FG+GRRICPGL MG   VE  LAN+LYHFDWKLP GM  E++DME
Sbjct: 422 DSNIDAKGQNFELLSFGSGRRICPGLYMGTTMVEFGLANMLYHFDWKLPEGMVVEDIDME 481

BLAST of CmaCh18G007460 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 394.0 bits (1011), Expect = 1.8e-109
Identity = 199/443 (44.92%), Postives = 291/443 (65.69%), Query Frame = 0

Query: 121 YGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFSPYND 180
           YGP++ L LG +  ++ISS+E A A+LKT+DL+ C+RP+T    K +Y F D+ F+PY +
Sbjct: 62  YGPLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGE 121

Query: 181 YLREMRKIS----------------------------SCGAI----VDLSEKSIALTTGV 240
           Y RE+RK++                            S  A+    VDLS+   +LT  +
Sbjct: 122 YWREVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASI 181

Query: 241 IFRIAFGKPFEGDGF-------HELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREAR 300
           I R+A G+ F   GF        EL++E    +G+++ S+FFP   +G   DW   R  +
Sbjct: 182 ICRVALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFP-GGLGRFVDWLFQRHKK 241

Query: 301 IEKVFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAIL 360
           I KVF E++A +Q VID+HL PE  K    DI+ ++L +  KQ    +  +N +N+KAI+
Sbjct: 242 INKVFKELDAFYQHVIDDHLKPEGRK--NQDIVTLILDMIDKQEDSDSFKLNMDNLKAIV 301

Query: 361 FNIFVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYV-RNKQKVREKDVEELPYLKM 420
            ++F+AG+DT ++T++WAM EL +N ++MKKAQE IR  +   K+++ E+D+ ++ YL  
Sbjct: 302 MDVFLAGIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEYLNH 361

Query: 421 IVKETLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPE 480
           I+KET RLHP  P ++PRET+SH KI+ YD  PKT++Q+N+W IGRDP  W DPEEF PE
Sbjct: 362 ILKETFRLHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEFNPE 421

Query: 481 RFAESSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEEL 524
           RFA SSVD++GQH++ LPFG+GRRICPG+ M + +VELAL NLLY+FDW +P+G K E++
Sbjct: 422 RFANSSVDFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTKGEDI 481

BLAST of CmaCh18G007460 vs. TAIR 10
Match: AT5G57260.1 (cytochrome P450, family 71, subfamily B, polypeptide 10 )

HSP 1 Score: 388.7 bits (997), Expect = 7.7e-108
Identity = 201/443 (45.37%), Postives = 281/443 (63.43%), Query Frame = 0

Query: 121 YGPIMSLNLGRIETIIISSTETARALLKTHDLQSCNRPQTHAMKKFTYNFLDLGFSPYND 180
           YGP+M L LGR+ T+I+S+ ETA+ +LK +DL  C+RP     +K +YN+LD+ FS ++D
Sbjct: 61  YGPVMLLKLGRVPTVIVSTPETAKQVLKDYDLHCCSRPSLEGTRKLSYNYLDIAFSRFDD 120

Query: 181 YLREMRKI--------------------------------SSCGAIVDLSEKSIALTTGV 240
           Y +E+RK+                                +S   +V+LS+  ++L   V
Sbjct: 121 YWKELRKLCVEELFCNKRINSIQPIKEAEMEKLIDSIAESASQKTLVNLSDTFLSLNVNV 180

Query: 241 IFRIAFGKPFEG-----DGFHELISEVEALMGSYSASEFFPVPFVGEVFDWFSGREARIE 300
           I +  FG  F+G     D F +L+ E   ++GS+SAS+FF  P+VG + DWF+G  AR E
Sbjct: 181 ICKAVFGVNFQGTVLNNDKFQDLVHEALEMLGSFSASDFF--PYVGWIVDWFTGLHARRE 240

Query: 301 KVFNEINALFQKVIDEHLCPERPKPEQDDIIDVLLAIGKKQVHPHTVVINHENIKAILFN 360
           +   +++A ++++ID HL   R + E DD +D+LL + K++       +   +IKAIL N
Sbjct: 241 RSVRDLDAFYEQMIDLHLQKNREESE-DDFVDLLLRLEKEEAVLGYGKLTRNHIKAILMN 300

Query: 361 IFVAGLDTGSITIVWAMAELAKNAKLMKKAQEEIRNYVRNKQKVREKDVEE---LPYLKM 420
           I + G++T +IT+ WAMAEL +N ++MKK Q EIR  +    K R   ++E   L YL M
Sbjct: 301 ILLGGINTSAITMTWAMAELIRNPRVMKKVQSEIRAQIGKNNKTRIISLDEINHLSYLNM 360

Query: 421 IVKETLRLHPPAPLLLPRETISHFKIEDYDFYPKTKVQVNIWAIGRDPTYWTDPEEFLPE 480
           ++KET RLHP APLL+PRE IS FKI  Y   PKT++ VN+WAIGRDP  W DPEEFLPE
Sbjct: 361 VIKETCRLHPVAPLLVPREVISEFKINGYTIQPKTRLHVNVWAIGRDPEIWKDPEEFLPE 420

Query: 481 RFAESSVDYKGQHYEFLPFGAGRRICPGLNMGVKTVELALANLLYHFDWKLPNGMKEEEL 524
           RF +  +D KGQ YE LPFG+GRRICP + MG+ TVE  LANLLYHFDWKLP G+  E++
Sbjct: 421 RFMDCDIDVKGQDYELLPFGSGRRICPAVYMGITTVEFGLANLLYHFDWKLPEGVAVEDI 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LTM46.6e-11245.96Cytochrome P450 71B19 OS=Arabidopsis thaliana OX=3702 GN=CYP71B19 PE=1 SV=1[more]
Q9LTM31.2e-11045.96Cytochrome P450 71B20 OS=Arabidopsis thaliana OX=3702 GN=CYP71B20 PE=2 SV=1[more]
Q9LTL01.5e-10845.48Cytochrome P450 71B26 OS=Arabidopsis thaliana OX=3702 GN=CYP71B26 PE=2 SV=1[more]
O657882.6e-10844.92Cytochrome P450 71B2 OS=Arabidopsis thaliana OX=3702 GN=CYP71B2 PE=2 SV=2[more]
Q9LVD21.1e-10645.37Cytochrome P450 71B10 OS=Arabidopsis thaliana OX=3702 GN=CYP71B10 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HQM21.9e-23191.87cytochrome P450 71B19-like OS=Cucurbita maxima OX=3661 GN=LOC111466486 PE=3 SV=1[more]
A0A6J1FZI81.7e-20882.17cytochrome P450 71B34-like OS=Cucurbita moschata OX=3662 GN=LOC111449355 PE=3 SV... [more]
A0A6J1HTM01.1e-20781.72cytochrome P450 71B34-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146648... [more]
A0A6J1HTN15.3e-20581.26cytochrome P450 71B34-like OS=Cucurbita maxima OX=3661 GN=LOC111466493 PE=3 SV=1[more]
A0A6J1G0A51.6e-20180.36cytochrome P450 71B19-like OS=Cucurbita moschata OX=3662 GN=LOC111449539 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_022966921.14.0e-23191.87cytochrome P450 71B19-like [Cucurbita maxima][more]
XP_023542050.12.0e-21485.10cytochrome P450 71B19-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022944978.13.6e-20882.17cytochrome P450 71B34-like [Cucurbita moschata][more]
XP_022966918.12.3e-20781.72cytochrome P450 71B34-like isoform X1 [Cucurbita maxima][more]
XP_023542080.17.5e-20680.59cytochrome P450 71B19-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G26170.14.7e-11345.96cytochrome P450, family 71, subfamily B, polypeptide 19 [more]
AT3G26180.18.8e-11245.96cytochrome P450, family 71, subfamily B, polypeptide 20 [more]
AT3G26290.11.1e-10945.48cytochrome P450, family 71, subfamily B, polypeptide 26 [more]
AT1G13080.11.8e-10944.92cytochrome P450, family 71, subfamily B, polypeptide 2 [more]
AT5G57260.17.7e-10845.37cytochrome P450, family 71, subfamily B, polypeptide 10 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 343..370
NoneNo IPR availablePANTHERPTHR47956:SF17CYTOCHROME P450coord: 43..82
coord: 128..189
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 43..82
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 128..189
coord: 190..524
NoneNo IPR availablePANTHERPTHR47956:SF17CYTOCHROME P450coord: 190..524
IPR001128Cytochrome P450PRINTSPR00385P450coord: 457..466
score: 62.62
coord: 327..344
score: 37.02
coord: 380..391
score: 40.84
IPR001128Cytochrome P450PFAMPF00067p450coord: 182..510
e-value: 6.9E-75
score: 252.6
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 118..137
score: 26.82
coord: 420..444
score: 38.91
coord: 456..466
score: 62.02
coord: 316..333
score: 34.71
coord: 379..397
score: 34.1
coord: 336..362
score: 26.58
coord: 466..489
score: 29.51
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 108..523
e-value: 2.4E-95
score: 321.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 119..522
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 459..468

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G007460.1CmaCh18G007460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen