CmaCh18G007310 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh18G007310 vs. ExPASy Swiss-Prot
Match: A1L4V7 (D-cysteine desulfhydrase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g26115 PE=2 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 2.1e-128
Identity = 243/426 (57.04%), Postives = 309/426 (72.54%), Query Frame = 0

Query: 72  SRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKV-ILSTEGTDIKDSPANHSCLA 131
           S+++  +S++      +FV++LLDRKW L  P S I ++ + S +G D K S  N++   
Sbjct: 24  SQRIPITSEI---DSREFVSKLLDRKWGLQCPASPIQQISVSSVKGID-KFSFLNNTRPH 83

Query: 132 VDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCG 191
           +        D   ++    SFY++RDDLLHPL+NGNKARKLD  LPL+ED+ VTD+VTCG
Sbjct: 84  L-------GDEMSKSKQGSSFYILRDDLLHPLVNGNKARKLDALLPLVEDHKVTDLVTCG 143

Query: 192 GCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKM 251
           GCQSAH AA AV CAERGLRSHLLLRGEQP++LTGYNL+ST+YGNV YVPRS YANRE+M
Sbjct: 144 GCQSAHTAAVAVSCAERGLRSHLLLRGEQPDVLTGYNLVSTMYGNVQYVPRSRYANREEM 203

Query: 252 LETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKHERKVIIINEG 311
           L T A+LVAG  G++LW  DI +         T N  ++D DF    +   RKV+I+NEG
Sbjct: 204 LRTYADLVAGEDGTVLWAKDIVEGR------DTMNVAKMD-DFS-SMKTSRRKVLIVNEG 263

Query: 312 AGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALCLELPWEVTAV 371
           AGD++ALLG+ RLV +LSQDHLLGK+R +KFVVDAGTGTTA+GLG+ A+ L LPWE+ AV
Sbjct: 264 AGDALALLGMFRLVQHLSQDHLLGKKRPVKFVVDAGTGTTAVGLGVAAMSLGLPWEINAV 323

Query: 372 MLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRKFGNVLVGEVE 431
           MLAD +  YKR E  LI+EF + F   V     ++N  ++ W++R  PRKFG VL GEVE
Sbjct: 324 MLADTLKNYKRHEDHLIAEFSRQFPGSVFCSGLDMN-QMIKWIDRQHPRKFGKVLEGEVE 383

Query: 432 TCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGMFGIAQRYKSY 491
            C++IAQETG+LVDP+YTLAAWE AT L Q   +  + VVMLHTGGTLGMFG+AQRYK+ 
Sbjct: 384 MCRKIAQETGVLVDPMYTLAAWETATELVQD--EKSSIVVMLHTGGTLGMFGLAQRYKTC 427

Query: 492 FNNLKN 497
           F NLK+
Sbjct: 444 FTNLKD 427

BLAST of CmaCh18G007310 vs. ExPASy Swiss-Prot
Match: B9EYZ1 (Putative D-cysteine desulfhydrase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0695600 PE=3 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 1.2e-107
Identity = 211/412 (51.21%), Postives = 267/412 (64.81%), Query Frame = 0

Query: 85  GGEDFVARLLD-RKWALANPDSKINKVILSTEGTDIKDSPANHSCLAVDTNICTYNDLAR 144
           GG   VA LL   +W L +P ++++ +  S   +    SP +H   +  T     N    
Sbjct: 9   GGGRTVANLLSATEWMLPSPATQVHTI--SVLPSHSPPSPPHHFAFSNLTTAPKRNGGKG 68

Query: 145 ENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCGGCQSAHAAATAVL 204
           E      F VVRDDLLHPL NGNKARKLD  LPL+     TDVVTCGGCQSAHAAATAV 
Sbjct: 69  EEEGRPRFEVVRDDLLHPLANGNKARKLDALLPLLRRRGATDVVTCGGCQSAHAAATAVH 128

Query: 205 CAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKMLETRAELVAGSSG 264
           CAE G+R H+LLRGEQP++ TGYNL+S ++GNV Y  RS+YA+R++ML   A  VAG+ G
Sbjct: 129 CAEWGMRPHILLRGEQPDIPTGYNLISLMFGNVAYASRSVYAHRDEMLYNHARKVAGTGG 188

Query: 265 SILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKHERKVIIINEGAGDSIALLGLIRL 324
           ++LW DDI +   +       N  ++            R+V+II EGAGD  ALLG+IRL
Sbjct: 189 TVLWADDISKEDFV---LDEDNGCEIG----------SRRVVIIKEGAGDVQALLGVIRL 248

Query: 325 VNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALCLELPWEVTAVMLADRIDGYKRQE 384
           V YL       K   +  VVDAGTGTTA+GL LGA+CL L W VTAVMLAD ++ YK +E
Sbjct: 249 VEYLYNLSSFHKHENVHVVVDAGTGTTAVGLALGAVCLGLHWRVTAVMLADTLERYKERE 308

Query: 385 KRLISEFKK--HFGYPVGLGEDEVNGGIVNWVERFRPRKFGNVLVGEVETCKQIAQETGI 444
           K LIS+FKK  H  Y   +GE+++   +V WVERF PR+FG VL GE+  C+QIAQ+TGI
Sbjct: 309 KSLISDFKKLCHNNYHEMVGENDIGDSLVEWVERFSPRRFGKVLNGEIALCRQIAQQTGI 368

Query: 445 LVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGMFGIAQRYKSYFNN 494
           L+DP+YTLA WE A  L    ++ K  VVM+HTGGTLG  G+AQRY S+F +
Sbjct: 369 LLDPMYTLAGWEQAVDLCVGDSRTK--VVMIHTGGTLGFCGLAQRYSSHFTS 403

BLAST of CmaCh18G007310 vs. ExPASy Swiss-Prot
Match: Q9WY68 (Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=TM_0225 PE=3 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.5e-20
Identity = 98/339 (28.91%), Postives = 144/339 (42.48%), Query Frame = 0

Query: 150 SFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCGGCQSAHAAATAVLCAERGL 209
           + YV RDDL   + +GNK RKL+  L        T V TCGG QS HA ATA +    GL
Sbjct: 25  NIYVKRDDLTELVGSGNKIRKLEYLLWEALKKGATTVFTCGGLQSNHARATAYVSRRYGL 84

Query: 210 RSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKMLETRAELVAGSSGSILWFD 269
           +  L LR +  ++L G  L+  + G                    AE+V  S       D
Sbjct: 85  KPVLFLR-KGEKVLNGNLLLDILLG--------------------AEIVEVSPEEYERID 144

Query: 270 DIFQSSIMKQPCSTTNSVQVDYDFHIRAR-KHERKVIIINEGAGDSIALLGLIRLVNYLS 329
           +IF                   D H + R K   KV +I EG  +S+   G    V  L 
Sbjct: 145 EIF-------------------DVHKKMREKKGEKVYVIPEGGSNSLGAFGYFNAV--LE 204

Query: 330 QDHLLGKQRAIKFVVDAGTGTTAIGLGLGALCLELPWEVTAVMLADR-IDGYKRQEKRLI 389
               L  +     V   G+G T  GL  G   LE    V  V +  +  D +  + KR+I
Sbjct: 205 MKDQLNLESFDAIVCAVGSGGTIAGLSAGISFLEYHVPVVGVNVTTKNSDYFVGKVKRII 264

Query: 390 SEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRKFGNVLVGEVETCKQIAQETGILVDPIY 449
           S  +++     GL    VN  +   V+ +R   +      +VE  K++A   GI++DP+Y
Sbjct: 265 SGMEEY-----GL---RVNETVFEVVDDYRGPGYAIPSSEDVEILKEVASIEGIILDPVY 310

Query: 450 TLAAWEMATFLSQKGAKVKADVVMLHTGGTLGMFGIAQR 487
           T  A+     + +   K   +V+ +HTGG  G+F  ++R
Sbjct: 325 TAKAFRGMIEMFRNSEK---NVLFIHTGGIFGLFAQSRR 310

BLAST of CmaCh18G007310 vs. ExPASy Swiss-Prot
Match: Q9V2L2 (Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=PYRAB00630 PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 6.8e-18
Identity = 92/332 (27.71%), Postives = 144/332 (43.37%), Query Frame = 0

Query: 152 YVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCGGCQSAHAAATAVLCAERGLRS 211
           YV RDDL    I GNK RKL+  L          V+T G   S HA  TA+   + GL +
Sbjct: 39  YVKRDDLTGLGIGGNKIRKLEFLLGDALSRGCDTVITIGAVHSNHAFVTALAAKKLGLGA 98

Query: 212 HLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKMLETRAELVAGSSGSILWFDDI 271
            L+LRGE  E+L G  L+  + G                +ETR                I
Sbjct: 99  VLILRGE--EVLKGNYLLDKLMG----------------IETR----------------I 158

Query: 272 FQSSIMKQPCSTTNSVQVDYDFHIRARKHERKVIIINEGAGDSIALLGLIRLVNYL-SQD 331
           +++       ++   ++V  +     +   +K  II  G    +  LG IR V  L +Q 
Sbjct: 159 YEAD------NSWELMKVAEEVAEELKGEGKKPYIIPPGGASPVGTLGYIRGVGELYTQV 218

Query: 332 HLLGKQRAIKFVVDA-GTGTTAIGLGLGALCLELPWEVTAVMLADRIDGYKRQEKRLISE 391
             LG +  I  VVDA G+G T  GL LG+  +   W V  + ++   +  K + K L+ +
Sbjct: 219 KKLGLR--IDTVVDAVGSGGTYAGLLLGSAIVNAEWSVVGIDVSSATEKAKERVKNLVEK 278

Query: 392 FKKHFGYPVGLGEDEVNGGIVNWVERFRPRKFGNVLVGEVETCKQIAQETGILVDPIYTL 451
            K+  G  V + E  +          +    +G ++    +  K +    G+L+DP+YT 
Sbjct: 279 TKELLGINVKVQEPRIYD--------YGFGAYGKIVKEVAKLIKSVGTMEGLLLDPVYTG 319

Query: 452 AAWEMATFLSQKGAKVKADVVMLHTGGTLGMF 482
            A+     L++KG  +   V+ +HTGG  G+F
Sbjct: 339 KAFYGLMDLAKKG-DLGESVLFIHTGGLPGIF 319

BLAST of CmaCh18G007310 vs. ExPASy Swiss-Prot
Match: F4HYF3 (Bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DCD PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.0e-14
Identity = 93/385 (24.16%), Postives = 163/385 (42.34%), Query Frame = 0

Query: 119 IKDSPANHSCLA-VDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPL 178
           ++  P++   LA + T I  +N     N +E   ++ RDD     ++GNK RKL+  +  
Sbjct: 46  LRPLPSHTFSLAHLPTPIHRWNLPGLPNGTE--LWIKRDDFTGMELSGNKVRKLEFLMAE 105

Query: 179 IEDNLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVT 238
             D     V+T GG QS H  ATA       L SHL+LR    +LL   +    + GN+ 
Sbjct: 106 AVDQHADTVITIGGIQSNHCRATATASNYLNLNSHLILR--TSKLLADED--PGLVGNLL 165

Query: 239 YVPRSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRA 298
                     E+++     L++                  K+  S+  S  +      + 
Sbjct: 166 V---------ERLVGANVHLIS------------------KEEYSSIGSEALTNALKEKL 225

Query: 299 RKHERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKF---VVDAGTGTTAIGL 358
            K  +K  +I  G  +S+   G I     + ++ L  +   +KF   VV  G+G T  G+
Sbjct: 226 EKEGKKPYVIPVGGSNSLGTWGYIEAAREI-EEQLNYRPDDLKFDDIVVACGSGGTIAGI 285

Query: 359 GLGALCLELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVE 418
            LG+    L  +V A  + D  D +    + L+           GL     +  IVN + 
Sbjct: 286 SLGSWLGALKAKVHAFSVCDDPDYFYDFVQGLLD----------GLHAGVNSRDIVN-IH 345

Query: 419 RFRPRKFGNVLVGEVETCKQIAQETGILVDPIYT-LAAWEMATFLSQ-----KGAKVKAD 478
             + + +      E+E  K++A  TG+++DP+Y+  AA+ +   +++     +G K    
Sbjct: 346 NAKGKGYAMNTSEELEFVKKVASSTGVILDPVYSGKAAYGLINEITKDPKCWEGRK---- 381

Query: 479 VVMLHTGGTLGMFGIAQRYKSYFNN 494
           ++ +HTGG LG++    +  S   N
Sbjct: 406 ILFIHTGGLLGLYDKVDQMASLMGN 381

BLAST of CmaCh18G007310 vs. ExPASy TrEMBL
Match: A0A6J1HST3 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466307 PE=3 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 4.7e-288
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0

Query: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60
           MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK
Sbjct: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60

Query: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120
           TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK
Sbjct: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120

Query: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300
           RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH
Sbjct: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300

Query: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360
           ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC
Sbjct: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK 420
           LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK
Sbjct: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK 420

Query: 421 FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480
           FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM
Sbjct: 421 FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480

Query: 481 FGIAQRYKSYFNNLKNV 498
           FGIAQRYKSYFNNLKNV
Sbjct: 481 FGIAQRYKSYFNNLKNV 497

BLAST of CmaCh18G007310 vs. ExPASy TrEMBL
Match: A0A6J1HUH2 (D-cysteine desulfhydrase 2, mitochondrial isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466307 PE=3 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.6e-283
Identity = 492/497 (98.99%), Postives = 492/497 (98.99%), Query Frame = 0

Query: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60
           MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK
Sbjct: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60

Query: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120
           TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK
Sbjct: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120

Query: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAAT     ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAAT-----ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300
           RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH
Sbjct: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300

Query: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360
           ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC
Sbjct: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK 420
           LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK
Sbjct: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK 420

Query: 421 FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480
           FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM
Sbjct: 421 FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480

Query: 481 FGIAQRYKSYFNNLKNV 498
           FGIAQRYKSYFNNLKNV
Sbjct: 481 FGIAQRYKSYFNNLKNV 492

BLAST of CmaCh18G007310 vs. ExPASy TrEMBL
Match: A0A6J1FZ47 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449244 PE=3 SV=1)

HSP 1 Score: 934.1 bits (2413), Expect = 2.4e-268
Identity = 465/497 (93.56%), Postives = 481/497 (96.78%), Query Frame = 0

Query: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60
           MRQAIPKMASA SVGSFTS P  PR+ SSVFRPRRTVVQSR+GGSGFFFR TK RLLHSK
Sbjct: 1   MRQAIPKMASARSVGSFTSLPYRPRDASSVFRPRRTVVQSRMGGSGFFFRDTKCRLLHSK 60

Query: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120
           TA  AMDNEY SRKLT+SSQVVQFGGEDFVARLLDR+WALANPDSKINKVILSTE T+++
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRQWALANPDSKINKVILSTEDTNLE 120

Query: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDL+R NASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300
           RSIYANREKML+T+AELVAGSSGSILWFDD+FQSSIMKQPCSTTNS+ VDYDFHIRARK+
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSLPVDYDFHIRARKY 300

Query: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360
           ERKV++INEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC
Sbjct: 301 ERKVVVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK 420
           L LPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYP+GLGEDEVNGGIVNWVERFRPRK
Sbjct: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPIGLGEDEVNGGIVNWVERFRPRK 420

Query: 421 FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480
           FGNVL GEVE CKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM
Sbjct: 421 FGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480

Query: 481 FGIAQRYKSYFNNLKNV 498
           FGIAQRYKSYFNNLKNV
Sbjct: 481 FGIAQRYKSYFNNLKNV 497

BLAST of CmaCh18G007310 vs. ExPASy TrEMBL
Match: A0A6J1FZ73 (D-cysteine desulfhydrase 2, mitochondrial isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449244 PE=3 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 8.0e-264
Identity = 460/497 (92.56%), Postives = 476/497 (95.77%), Query Frame = 0

Query: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60
           MRQAIPKMASA SVGSFTS P  PR+ SSVFRPRRTVVQSR+GGSGFFFR TK RLLHSK
Sbjct: 1   MRQAIPKMASARSVGSFTSLPYRPRDASSVFRPRRTVVQSRMGGSGFFFRDTKCRLLHSK 60

Query: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120
           TA  AMDNEY SRKLT+SSQVVQFGGEDFVARLLDR+WALANPDSKINKVILSTE T+++
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRQWALANPDSKINKVILSTEDTNLE 120

Query: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDL+R NASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAAT     ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAAT-----ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300
           RSIYANREKML+T+AELVAGSSGSILWFDD+FQSSIMKQPCSTTNS+ VDYDFHIRARK+
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSLPVDYDFHIRARKY 300

Query: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360
           ERKV++INEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC
Sbjct: 301 ERKVVVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK 420
           L LPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYP+GLGEDEVNGGIVNWVERFRPRK
Sbjct: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPIGLGEDEVNGGIVNWVERFRPRK 420

Query: 421 FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480
           FGNVL GEVE CKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM
Sbjct: 421 FGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480

Query: 481 FGIAQRYKSYFNNLKNV 498
           FGIAQRYKSYFNNLKNV
Sbjct: 481 FGIAQRYKSYFNNLKNV 492

BLAST of CmaCh18G007310 vs. ExPASy TrEMBL
Match: A0A6J1DFQ3 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019685 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 4.6e-219
Identity = 382/491 (77.80%), Postives = 433/491 (88.19%), Query Frame = 0

Query: 8   MASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSKTAKVAMD 67
           M SA SV SF S P+ P +VS V R  RTVV+S +GG G+FF ATK RLLHSKTA  A+D
Sbjct: 1   MGSARSVCSFMSVPSRPGDVSGVLRWWRTVVESEMGGPGYFFSATKLRLLHSKTAIAAVD 60

Query: 68  NEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIKDSPANHS 127
           NE+  R  T  SQ V+ GGE+FV+RLLDR+W LA+PD+KI+KV  S   T++++SPA+H 
Sbjct: 61  NEFSFRNFTDGSQGVKLGGEEFVSRLLDRRWTLADPDTKISKVTFSNADTNLENSPASHL 120

Query: 128 CLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVV 187
           CLAVDTN CT+ND++R +  EQSFYVVRDDLLHPLINGNKARKLDG LPLIEDN VTDVV
Sbjct: 121 CLAVDTNSCTFNDVSRTDYQEQSFYVVRDDLLHPLINGNKARKLDGVLPLIEDNSVTDVV 180

Query: 188 TCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANR 247
           TCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNL+STIYGNVTYVPR+IYANR
Sbjct: 181 TCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLISTIYGNVTYVPRAIYANR 240

Query: 248 EKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKHERKVIII 307
           EKML+++A+LVAGSS  I+WFDDIF SS+ KQ CS TNSVQ+DYDF+ RARKH RKVI+I
Sbjct: 241 EKMLKSQADLVAGSSDGIIWFDDIFPSSLKKQACS-TNSVQIDYDFNRRARKHGRKVIVI 300

Query: 308 NEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALCLELPWEV 367
           NEGAGD++ALLGLIRLV YLSQDHLLGKQRA+KFVVDAGTGTTA+GLGLGALCL LPWEV
Sbjct: 301 NEGAGDAVALLGLIRLVKYLSQDHLLGKQRAVKFVVDAGTGTTAVGLGLGALCLGLPWEV 360

Query: 368 TAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRKFGNVLVG 427
           TA+MLAD+IDGYK+QEKRLISEF+K+FGYP  LG + VNG +VNWVERF PRKFGNVL G
Sbjct: 361 TALMLADKIDGYKQQEKRLISEFRKYFGYPADLGNNVVNGAVVNWVERFHPRKFGNVLDG 420

Query: 428 EVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKAD-VVMLHTGGTLGMFGIAQR 487
           EVETC+QIAQ+TGILVDPIYTLAAWEMA+FLSQKGAK  AD VV+LHTGGTLGMFG+AQR
Sbjct: 421 EVETCRQIAQKTGILVDPIYTLAAWEMASFLSQKGAKTNADVVVLLHTGGTLGMFGLAQR 480

Query: 488 YKSYFNNLKNV 498
           YKSYFN+LKNV
Sbjct: 481 YKSYFNDLKNV 490

BLAST of CmaCh18G007310 vs. NCBI nr
Match: XP_022966678.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 999.6 bits (2583), Expect = 9.7e-288
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0

Query: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60
           MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK
Sbjct: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60

Query: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120
           TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK
Sbjct: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120

Query: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300
           RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH
Sbjct: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300

Query: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360
           ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC
Sbjct: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK 420
           LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK
Sbjct: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK 420

Query: 421 FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480
           FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM
Sbjct: 421 FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480

Query: 481 FGIAQRYKSYFNNLKNV 498
           FGIAQRYKSYFNNLKNV
Sbjct: 481 FGIAQRYKSYFNNLKNV 497

BLAST of CmaCh18G007310 vs. NCBI nr
Match: XP_022966679.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X2 [Cucurbita maxima])

HSP 1 Score: 984.6 bits (2544), Expect = 3.2e-283
Identity = 492/497 (98.99%), Postives = 492/497 (98.99%), Query Frame = 0

Query: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60
           MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK
Sbjct: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60

Query: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120
           TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK
Sbjct: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120

Query: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAAT     ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAAT-----ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300
           RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH
Sbjct: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300

Query: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360
           ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC
Sbjct: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK 420
           LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK
Sbjct: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK 420

Query: 421 FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480
           FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM
Sbjct: 421 FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480

Query: 481 FGIAQRYKSYFNNLKNV 498
           FGIAQRYKSYFNNLKNV
Sbjct: 481 FGIAQRYKSYFNNLKNV 492

BLAST of CmaCh18G007310 vs. NCBI nr
Match: XP_022944821.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 934.1 bits (2413), Expect = 5.0e-268
Identity = 465/497 (93.56%), Postives = 481/497 (96.78%), Query Frame = 0

Query: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60
           MRQAIPKMASA SVGSFTS P  PR+ SSVFRPRRTVVQSR+GGSGFFFR TK RLLHSK
Sbjct: 1   MRQAIPKMASARSVGSFTSLPYRPRDASSVFRPRRTVVQSRMGGSGFFFRDTKCRLLHSK 60

Query: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120
           TA  AMDNEY SRKLT+SSQVVQFGGEDFVARLLDR+WALANPDSKINKVILSTE T+++
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRQWALANPDSKINKVILSTEDTNLE 120

Query: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDL+R NASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300
           RSIYANREKML+T+AELVAGSSGSILWFDD+FQSSIMKQPCSTTNS+ VDYDFHIRARK+
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSLPVDYDFHIRARKY 300

Query: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360
           ERKV++INEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC
Sbjct: 301 ERKVVVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK 420
           L LPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYP+GLGEDEVNGGIVNWVERFRPRK
Sbjct: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPIGLGEDEVNGGIVNWVERFRPRK 420

Query: 421 FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480
           FGNVL GEVE CKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM
Sbjct: 421 FGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480

Query: 481 FGIAQRYKSYFNNLKNV 498
           FGIAQRYKSYFNNLKNV
Sbjct: 481 FGIAQRYKSYFNNLKNV 497

BLAST of CmaCh18G007310 vs. NCBI nr
Match: XP_023541195.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 922.2 bits (2382), Expect = 2.0e-264
Identity = 463/499 (92.79%), Postives = 477/499 (95.59%), Query Frame = 0

Query: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60
           MRQAIPKMAS  SVGSFTS PN PR+VSSVFR RRTVVQSR+GG GFFFRATK RLLHSK
Sbjct: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60

Query: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120
           TA  AMDNEY SRKLT+SSQVVQFGGEDFVARLLDR+WALANP+SKINKVIL T  T+++
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120

Query: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
            SPANH CLAVDTNICTYNDL+R NASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300
           RSIYANREKML+T+AELVAGSSGSILWFDD+FQSSIMKQPCSTTNSV VDYDFHIRARKH
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300

Query: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360
           ERKVI+INEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFV+DAGTGTTAIGLGLGALC
Sbjct: 301 ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC 360

Query: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYP--VGLGEDEVNGGIVNWVERFRP 420
           L LPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYP  VGLGEDEVNGGIVNWVERFRP
Sbjct: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP 420

Query: 421 RKFGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480
           RKFGNVL GEVE CKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL
Sbjct: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480

Query: 481 GMFGIAQRYKSYFNNLKNV 498
           GMFGIAQRYKSYFNNLKNV
Sbjct: 481 GMFGIAQRYKSYFNNLKNV 499

BLAST of CmaCh18G007310 vs. NCBI nr
Match: XP_022944822.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X2 [Cucurbita moschata])

HSP 1 Score: 919.1 bits (2374), Expect = 1.7e-263
Identity = 460/497 (92.56%), Postives = 476/497 (95.77%), Query Frame = 0

Query: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60
           MRQAIPKMASA SVGSFTS P  PR+ SSVFRPRRTVVQSR+GGSGFFFR TK RLLHSK
Sbjct: 1   MRQAIPKMASARSVGSFTSLPYRPRDASSVFRPRRTVVQSRMGGSGFFFRDTKCRLLHSK 60

Query: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120
           TA  AMDNEY SRKLT+SSQVVQFGGEDFVARLLDR+WALANPDSKINKVILSTE T+++
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRQWALANPDSKINKVILSTEDTNLE 120

Query: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           DSPANHSCLAVDTNICTYNDL+R NASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAAT     ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAAT-----ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300
           RSIYANREKML+T+AELVAGSSGSILWFDD+FQSSIMKQPCSTTNS+ VDYDFHIRARK+
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSLPVDYDFHIRARKY 300

Query: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360
           ERKV++INEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC
Sbjct: 301 ERKVVVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRK 420
           L LPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYP+GLGEDEVNGGIVNWVERFRPRK
Sbjct: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPIGLGEDEVNGGIVNWVERFRPRK 420

Query: 421 FGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480
           FGNVL GEVE CKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM
Sbjct: 421 FGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGM 480

Query: 481 FGIAQRYKSYFNNLKNV 498
           FGIAQRYKSYFNNLKNV
Sbjct: 481 FGIAQRYKSYFNNLKNV 492

BLAST of CmaCh18G007310 vs. TAIR 10
Match: AT3G26115.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 460.7 bits (1184), Expect = 1.5e-129
Identity = 243/426 (57.04%), Postives = 309/426 (72.54%), Query Frame = 0

Query: 72  SRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKV-ILSTEGTDIKDSPANHSCLA 131
           S+++  +S++      +FV++LLDRKW L  P S I ++ + S +G D K S  N++   
Sbjct: 24  SQRIPITSEI---DSREFVSKLLDRKWGLQCPASPIQQISVSSVKGID-KFSFLNNTRPH 83

Query: 132 VDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCG 191
           +        D   ++    SFY++RDDLLHPL+NGNKARKLD  LPL+ED+ VTD+VTCG
Sbjct: 84  L-------GDEMSKSKQGSSFYILRDDLLHPLVNGNKARKLDALLPLVEDHKVTDLVTCG 143

Query: 192 GCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKM 251
           GCQSAH AA AV CAERGLRSHLLLRGEQP++LTGYNL+ST+YGNV YVPRS YANRE+M
Sbjct: 144 GCQSAHTAAVAVSCAERGLRSHLLLRGEQPDVLTGYNLVSTMYGNVQYVPRSRYANREEM 203

Query: 252 LETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKHERKVIIINEG 311
           L T A+LVAG  G++LW  DI +         T N  ++D DF    +   RKV+I+NEG
Sbjct: 204 LRTYADLVAGEDGTVLWAKDIVEGR------DTMNVAKMD-DFS-SMKTSRRKVLIVNEG 263

Query: 312 AGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALCLELPWEVTAV 371
           AGD++ALLG+ RLV +LSQDHLLGK+R +KFVVDAGTGTTA+GLG+ A+ L LPWE+ AV
Sbjct: 264 AGDALALLGMFRLVQHLSQDHLLGKKRPVKFVVDAGTGTTAVGLGVAAMSLGLPWEINAV 323

Query: 372 MLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPRKFGNVLVGEVE 431
           MLAD +  YKR E  LI+EF + F   V     ++N  ++ W++R  PRKFG VL GEVE
Sbjct: 324 MLADTLKNYKRHEDHLIAEFSRQFPGSVFCSGLDMN-QMIKWIDRQHPRKFGKVLEGEVE 383

Query: 432 TCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGMFGIAQRYKSY 491
            C++IAQETG+LVDP+YTLAAWE AT L Q   +  + VVMLHTGGTLGMFG+AQRYK+ 
Sbjct: 384 MCRKIAQETGVLVDPMYTLAAWETATELVQD--EKSSIVVMLHTGGTLGMFGLAQRYKTC 427

Query: 492 FNNLKN 497
           F NLK+
Sbjct: 444 FTNLKD 427

BLAST of CmaCh18G007310 vs. TAIR 10
Match: AT3G26115.2 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 453.0 bits (1164), Expect = 3.1e-127
Identity = 242/432 (56.02%), Postives = 309/432 (71.53%), Query Frame = 0

Query: 72  SRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKV-ILSTEGTDIKDSPANHSCLA 131
           S+++  +S++      +FV++LLDRKW L  P S I ++ + S +G D K S  N++   
Sbjct: 24  SQRIPITSEI---DSREFVSKLLDRKWGLQCPASPIQQISVSSVKGID-KFSFLNNTRPH 83

Query: 132 VDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCG 191
           +        D   ++    SFY++RDDLLHPL+NGNKARKLD  LPL+ED+ VTD+VTCG
Sbjct: 84  L-------GDEMSKSKQGSSFYILRDDLLHPLVNGNKARKLDALLPLVEDHKVTDLVTCG 143

Query: 192 GCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKM 251
           GCQSAH AA AV CAERGLRSHLLLRGEQP++LTGYNL+ST+YGNV YVPRS YANRE+M
Sbjct: 144 GCQSAHTAAVAVSCAERGLRSHLLLRGEQPDVLTGYNLVSTMYGNVQYVPRSRYANREEM 203

Query: 252 LETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKHERKVIIINEG 311
           L T A+LVAG  G++LW  DI +         T N  ++D DF    +   RKV+I+NEG
Sbjct: 204 LRTYADLVAGEDGTVLWAKDIVEGR------DTMNVAKMD-DFS-SMKTSRRKVLIVNEG 263

Query: 312 AGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALCLELPWEVTAV 371
           AGD++ALLG+ RLV +LSQDHLLGK+R +KFVVDAGTGTTA+GLG+ A+ L LPWE+ AV
Sbjct: 264 AGDALALLGMFRLVQHLSQDHLLGKKRPVKFVVDAGTGTTAVGLGVAAMSLGLPWEINAV 323

Query: 372 MLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVERFRPR------KFGNV 431
           MLAD +  YKR E  LI+EF + F   V     ++N  ++ W++R  PR      +FG V
Sbjct: 324 MLADTLKNYKRHEDHLIAEFSRQFPGSVFCSGLDMN-QMIKWIDRQHPRNSFCSCRFGKV 383

Query: 432 LVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGMFGIA 491
           L GEVE C++IAQETG+LVDP+YTLAAWE AT L Q   +  + VVMLHTGGTLGMFG+A
Sbjct: 384 LEGEVEMCRKIAQETGVLVDPMYTLAAWETATELVQD--EKSSIVVMLHTGGTLGMFGLA 433

Query: 492 QRYKSYFNNLKN 497
           QRYK+ F NLK+
Sbjct: 444 QRYKTCFTNLKD 433

BLAST of CmaCh18G007310 vs. TAIR 10
Match: AT1G48420.1 (D-cysteine desulfhydrase )

HSP 1 Score: 82.0 bits (201), Expect = 1.5e-15
Identity = 93/385 (24.16%), Postives = 163/385 (42.34%), Query Frame = 0

Query: 119 IKDSPANHSCLA-VDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPL 178
           ++  P++   LA + T I  +N     N +E   ++ RDD     ++GNK RKL+  +  
Sbjct: 46  LRPLPSHTFSLAHLPTPIHRWNLPGLPNGTE--LWIKRDDFTGMELSGNKVRKLEFLMAE 105

Query: 179 IEDNLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVT 238
             D     V+T GG QS H  ATA       L SHL+LR    +LL   +    + GN+ 
Sbjct: 106 AVDQHADTVITIGGIQSNHCRATATASNYLNLNSHLILR--TSKLLADED--PGLVGNLL 165

Query: 239 YVPRSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRA 298
                     E+++     L++                  K+  S+  S  +      + 
Sbjct: 166 V---------ERLVGANVHLIS------------------KEEYSSIGSEALTNALKEKL 225

Query: 299 RKHERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKF---VVDAGTGTTAIGL 358
            K  +K  +I  G  +S+   G I     + ++ L  +   +KF   VV  G+G T  G+
Sbjct: 226 EKEGKKPYVIPVGGSNSLGTWGYIEAAREI-EEQLNYRPDDLKFDDIVVACGSGGTIAGI 285

Query: 359 GLGALCLELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGLGEDEVNGGIVNWVE 418
            LG+    L  +V A  + D  D +    + L+           GL     +  IVN + 
Sbjct: 286 SLGSWLGALKAKVHAFSVCDDPDYFYDFVQGLLD----------GLHAGVNSRDIVN-IH 345

Query: 419 RFRPRKFGNVLVGEVETCKQIAQETGILVDPIYT-LAAWEMATFLSQ-----KGAKVKAD 478
             + + +      E+E  K++A  TG+++DP+Y+  AA+ +   +++     +G K    
Sbjct: 346 NAKGKGYAMNTSEELEFVKKVASSTGVILDPVYSGKAAYGLINEITKDPKCWEGRK---- 381

Query: 479 VVMLHTGGTLGMFGIAQRYKSYFNN 494
           ++ +HTGG LG++    +  S   N
Sbjct: 406 ILFIHTGGLLGLYDKVDQMASLMGN 381