CmaCh18G007210 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh18G007210
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	mitotic spindle checkpoint protein MAD2
Location	Cma_Chr18: 6536358 .. 6538941 (+)
RNA-Seq Expression	CmaCh18G007210
Synteny	CmaCh18G007210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCCGTTGCCTATCCACTCAAGATTGTCTCTACGAGACGAACCCTTGCTCATCATTTTACACAGCTTTTAGTCGCCCCTCCTGCAAGTTGAAGCTCTCAAATCTTCTCAAATCCACTTGTTTTTCTTTTCATCGATTCGATTTCTTCATGATCTTGATTACCCCTTGCATTGATTAAAACCCTAGATCCGCTCCTCGTCTTAGAATCCAAGATTCAGGAACAAATCTTACTGTTTCTTTAGGTTAAACAAAATTTGAAATGGCTTCAAAAACAGCTTCCAAAGACATTATCACTCTTCGCGGTTCTGCAGCGATTGTCAGCGAGTTCTTCGGTAAATTATGCGCATTCTGAAACCCTTCTGATTTCAATTTTCTGTTTAGTTCGTTCGATTATCGATGAATACTCTTGGCTGATTTGTTGTTTTTCGTTTTTCTTGAAATTGCTCATTTAGGTTATGCGGCAAATAGGTATGAATATTGATTTCGTTTTCTGTGCCCTAATTTTCATTTTGGAATCATGTAAATGTGCATTTTCTCACTTACAAGCGGTGTGGATTTTAAATTTGTAGCATCCTCTACAATCGTGGGCTTTATCCGGAAGAAAGCTTTGCAAGGGTGAAGAAATATGGACTTCCAATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTAACTGCTCAGCTTTCTGGTAAGTACCCTTTCTCTAAATGAAGGATTCGCACTGTTCAACAGTTCAGTATCGTTTTTTTCCTTCAAATTTATTTTCAGTCGATCAGATTGTGGTTTTGAATCATGAAATTGGTTTTAGAATGGCTGGAAGCTGGGAAGTTGCAAAGAGTTGTGCTCGTTATAATGAGCAAAGCCAATAATGAGGTCCTTGAGAGGTGGAACTTTAGAATTGAAACTGACGGAGAGGTTGTAGAGCAAGGGTAATAATCTCAACCAAAAACTTCTCCTCCCTTTCATAAATTTCTGGATTTTGCATTGATTAGATGAATTGTTGCAGTGTGTCGAGGGAAAAGAGTGATAAAGAAATAATGAGAGAGATCCAGGCTATTATGAGACAGATTGCTTCAAGCATCACTTACTTGCCCTGCCTAGATGAACCCTGTAAGTGTTGGCAAACTGTACCATTATGTATCATTTATTACTTTGGGGATTGGTGTGACATGATATTGTTAATTATGGCCAGTTCTAAATCCATGGGATGTCATGTAATTTCCATCATGATCCATGGCCAGTTATAATGATTCATGAGTTTAAATGACAATTTCTAGGCCACATTCTGAATCGGTAGGATGTCATATGAATTTTTTCGTCATTAAAACATTACTTTTATAGCCACTCTGAACATAACTTGAATAGTTAGCCATGTGTCTCTTTTTAAGGGATTTGAGTTCTAAATCCCCAATGTCTAAGGCCGGTTCTGAATCCATGGGATGTCATGTAGTTTCCATCATGATATCATTATCATCATGCAAGTGTTGCTTCAAGCATCATTTTCTTGTCCAACCGAGATGAGAACCCTACATTGGTTATTTTCAGAGGATTGGCGTTTTCATCATTAAAACATTACTTTTATAGCCACTCTGAACATAACTTGAATAGTTAGCCATGTGTCTCTTTTTAAGGGATTTGAGTTCTAAATCCCCAATGTCTAAGGCCGGTTCTGAATCCATGGGATGTCATGTAGTTTCCATCATGATATCATTATCATCATGCAAGTGTTGCTTCAAGCATCATTTTCTTGTCCAACCGAGATGAGAACCCTACATTGGTTATTTTCAGAGGATTGGCGTAACACGACGATTGTTAGGCCGCAATCTGAACCAATGAGATTTGAGTTCTATAGTTTGAATAGTTAGGTATGTGTGTGTGAGATCCATCCGTTGGAGAAGGGAACGAAACATTTCTTATAAGAGAAAGCCCAAAAAGGACAATATCTGCTAACGGTGGGCTTGGAGTGTGTCATTATGGGATTTGCATTCTGATCTCCCTTGTTTATATTCTAACACGCAATCATTAGTTCATAATGATTCATGAGTTTAAAAGATAATGTAGATGTGTGAGATCCCTTTTGCTTGTTGATGATAATCACCATGATGCTCAATCAAGTGTTCTCTGATGTTTTGTTGTCTCAGGTGTATTTGATGTGTTAGCATACACTGATAAAGATCTAGCAGTTCCATTTACTTGGATCGAGAGTGACCCCAAACTGATTGCTAATCCACAAATGGTGAAGCTCCATTCTTTCGATACAAAGGTGAGCAAATGGCATAACTCATTTTGTTCAGCTCACCCCTGCAAGCAACCAACTTAAACATTATAAATGGTGTTGATGCAGATTCACAAAGTGGACACTCTTGTTTCTTACAAGAACGATGACTGCGATGACGAGTAAGAACAACTCCTCCTCCTCCCTTTTCTTGAATTTGTTGTTAGTTTTCTGGATCCCTCCAACCCGATCGCTTTCAAGGTGGCATTGTGAATCTTTTATTCGCATGTTGTATTGTAATATCAATTAGGATTCCACCTTGAACGGTTGGTTGGTTGGTTGGTTGGTTGGTTGG

mRNA sequence

ATGAGCCGTTGCCTATCCACTCAAGATTGTCTCTACGAGACGAACCCTTGCTCATCATTTTACACAGCTTTTAGTCGCCCCTCCTGCAAGTTGAAGCTCTCAAATCTTCTCAAATCCACTTGTTTTTCTTTTCATCGATTCGATTTCTTCATGATCTTGATTACCCCTTGCATTGATTAAAACCCTAGATCCGCTCCTCGTCTTAGAATCCAAGATTCAGGAACAAATCTTACTGTTTCTTTAGGTTAAACAAAATTTGAAATGGCTTCAAAAACAGCTTCCAAAGACATTATCACTCTTCGCGGTTCTGCAGCGATTGTCAGCGAGTTCTTCGGTTATGCGGCAAATAGCATCCTCTACAATCGTGGGCTTTATCCGGAAGAAAGCTTTGCAAGGGTGAAGAAATATGGACTTCCAATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTAACTGCTCAGCTTTCTGAATGGCTGGAAGCTGGGAAGTTGCAAAGAGTTGTGCTCGTTATAATGAGCAAAGCCAATAATGAGGTCCTTGAGAGGTGGAACTTTAGAATTGAAACTGACGGAGAGGTTGTAGAGCAAGGTGTGTCGAGGGAAAAGAGTGATAAAGAAATAATGAGAGAGATCCAGGCTATTATGAGACAGATTGCTTCAAGCATCACTTACTTGCCCTGCCTAGATGAACCCTGTGTATTTGATGTGTTAGCATACACTGATAAAGATCTAGCAGTTCCATTTACTTGGATCGAGAGTGACCCCAAACTGATTGCTAATCCACAAATGGTGAAGCTCCATTCTTTCGATACAAAGATTCACAAAGTGGACACTCTTGTTTCTTACAAGAACGATGACTGCGATGACGAGTAAGAACAACTCCTCCTCCTCCCTTTTCTTGAATTTGTTGTTAGTTTTCTGGATCCCTCCAACCCGATCGCTTTCAAGGTGGCATTGTGAATCTTTTATTCGCATGTTGTATTGTAATATCAATTAGGATTCCACCTTGAACGGTTGGTTGGTTGGTTGGTTGGTTGGTTGG

Coding sequence (CDS)

ATGGCTTCAAAAACAGCTTCCAAAGACATTATCACTCTTCGCGGTTCTGCAGCGATTGTCAGCGAGTTCTTCGGTTATGCGGCAAATAGCATCCTCTACAATCGTGGGCTTTATCCGGAAGAAAGCTTTGCAAGGGTGAAGAAATATGGACTTCCAATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTAACTGCTCAGCTTTCTGAATGGCTGGAAGCTGGGAAGTTGCAAAGAGTTGTGCTCGTTATAATGAGCAAAGCCAATAATGAGGTCCTTGAGAGGTGGAACTTTAGAATTGAAACTGACGGAGAGGTTGTAGAGCAAGGTGTGTCGAGGGAAAAGAGTGATAAAGAAATAATGAGAGAGATCCAGGCTATTATGAGACAGATTGCTTCAAGCATCACTTACTTGCCCTGCCTAGATGAACCCTGTGTATTTGATGTGTTAGCATACACTGATAAAGATCTAGCAGTTCCATTTACTTGGATCGAGAGTGACCCCAAACTGATTGCTAATCCACAAATGGTGAAGCTCCATTCTTTCGATACAAAGATTCACAAAGTGGACACTCTTGTTTCTTACAAGAACGATGACTGCGATGACGAGTAA

Protein sequence

MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE

Homology

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BLAST of CmaCh18G007210 vs. ExPASy Swiss-Prot
Match: Q9LU93 (Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana OX=3702 GN=MAD2 PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 5.2e-97
Identity = 179/208 (86.06%), Postives = 194/208 (93.27%), Query Frame = 0


Query: 1   MASKT-ASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDE 60
           MASKT A+KDIITL GSAAIVSEFF YAANSILYNR +YPEESF +VKKYGLPMLL +DE
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFCYAANSILYNRAVYPEESFVKVKKYGLPMLLIEDE 60

Query: 61  GVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREK 120
            VK+F+SNLT+Q+SEWLEAGKLQRVVLVIMSKA  EVLERWNFRIETD EVV++GVSREK
Sbjct: 61  SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQ 180
           SDKEIMREIQAIMRQ+ASS+TYLPCLDE CVFDVLAYTD D+AVPFTWIESDPKLIANPQ
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQ 180

Query: 181 MVKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           MVKLH FDTKIHKVDTLVSYKND+ D+E
Sbjct: 181 MVKLHGFDTKIHKVDTLVSYKNDEWDEE 208

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BLAST of CmaCh18G007210 vs. ExPASy Swiss-Prot
Match: Q9XFH3 (Mitotic spindle checkpoint protein MAD2 OS=Zea mays OX=4577 GN=MAD2 PE=2 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 7.1e-94
Identity = 173/207 (83.57%), Postives = 189/207 (91.30%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MAS++ASKDIITLRGSAAIVSEFFGYAANSILYNR +YPEESF++VKKYGL MLLTQDEG
Sbjct: 1   MASRSASKDIITLRGSAAIVSEFFGYAANSILYNRAVYPEESFSKVKKYGLTMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120
           VK FI++LT+QLSEWLEAGKLQR+VLVIMSKA +EVLERWNF I TD EVVE+G  +EKS
Sbjct: 61  VKNFIASLTSQLSEWLEAGKLQRIVLVIMSKATSEVLERWNFNIVTDAEVVEKGAIKEKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIAS ITYLPCLDEPCVFDVLAYTD D+  P TW+ESD KLI NPQM
Sbjct: 121 DKEIMREIQAIMRQIASCITYLPCLDEPCVFDVLAYTDTDVDAPGTWVESDAKLIDNPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYK D+ D+E
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKKDEWDEE 207

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BLAST of CmaCh18G007210 vs. ExPASy Swiss-Prot
Match: Q556Y9 (Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum OX=44689 GN=mad2l1-1 PE=3 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 6.9e-57
Identity = 112/201 (55.72%), Postives = 145/201 (72.14%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           M +  ASK  I+L+GS  IV+EFF Y+ N+IL+ RGLYP ESF RV KYGLP+L+T D+ 
Sbjct: 1   MQAAVASKTNISLKGSTEIVTEFFSYSINTILFQRGLYPPESFTRVAKYGLPILVTNDQS 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQG-VSREK 120
           +K ++ N+  QLSEWL +G +Q++VLVI      EVLERW F + TD  + ++G   R+K
Sbjct: 61  LKDYLDNVLKQLSEWLLSGDVQKLVLVITDIVTKEVLERWVFDVTTD--IPKEGEAPRQK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQ 180
            +KEIM EIQAI+RQI +S+T+LP L   C FD+L YT KDLAVP  W ESDPK I N Q
Sbjct: 121 PEKEIMNEIQAIIRQITASVTFLPLLPNACTFDLLVYTSKDLAVPQKWEESDPKFITNSQ 180

Query: 181 MVKLHSFDTKIHKVDTLVSYK 201
            VKL SF T IHKV+++V+YK
Sbjct: 181 QVKLRSFTTTIHKVESMVAYK 199

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BLAST of CmaCh18G007210 vs. ExPASy Swiss-Prot
Match: Q9Z1B5 (Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus OX=10090 GN=Mad2l1 PE=1 SV=2)

HSP 1 Score: 206.8 bits (525), Expect = 2.3e-52
Identity = 103/200 (51.50%), Postives = 140/200 (70.00%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MA + A +  ITLRGSA IV+EFF +  NSILY RG+YP E+F RV+KYGL +L T D  
Sbjct: 1   MAQQLAREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLTTTDPE 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120
           +  +++N+  QL EWL    +Q++V+VI +  + EVLERW F IE D    E+GV REKS
Sbjct: 61  LIKYLNNVVEQLKEWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKEEGVRREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
            K I  EI++++RQI +++T+LP L+  C FD+L YTDKDL VP  W ES P+ I N + 
Sbjct: 121 QKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNCEE 180

Query: 181 VKLHSFDTKIHKVDTLVSYK 201
           V+L SF T IHKV+++V+YK
Sbjct: 181 VRLRSFTTTIHKVNSMVAYK 200

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BLAST of CmaCh18G007210 vs. ExPASy Swiss-Prot
Match: Q13257 (Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens OX=9606 GN=MAD2L1 PE=1 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 6.3e-50
Identity = 98/200 (49.00%), Postives = 138/200 (69.00%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MA + + +  ITLRGSA IV+EFF +  NSILY RG+YP E+F RV+KYGL +L+T D  
Sbjct: 1   MALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLE 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120
           +  +++N+  QL +WL    +Q++V+VI +  + EVLERW F IE D    +    REKS
Sbjct: 61  LIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
            K I  EI++++RQI +++T+LP L+  C FD+L YTDKDL VP  W ES P+ I N + 
Sbjct: 121 QKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEE 180

Query: 181 VKLHSFDTKIHKVDTLVSYK 201
           V+L SF T IHKV+++V+YK
Sbjct: 181 VRLRSFTTTIHKVNSMVAYK 200

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BLAST of CmaCh18G007210 vs. ExPASy TrEMBL
Match: A0A6J1HTK5 (mitotic spindle checkpoint protein MAD2 OS=Cucurbita maxima OX=3661 GN=LOC111466640 PE=3 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 1.5e-110
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDCDDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of CmaCh18G007210 vs. ExPASy TrEMBL
Match: A0A6J1GXP9 (mitotic spindle checkpoint protein MAD2 OS=Cucurbita moschata OX=3662 GN=LOC111458459 PE=3 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 1.5e-110
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDCDDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of CmaCh18G007210 vs. ExPASy TrEMBL
Match: A0A6J1KEW7 (mitotic spindle checkpoint protein MAD2-like OS=Cucurbita maxima OX=3661 GN=LOC111493236 PE=3 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 9.5e-110
Identity = 206/207 (99.52%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDG VVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDCDDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of CmaCh18G007210 vs. ExPASy TrEMBL
Match: A0A6J1FZG6 (mitotic spindle checkpoint protein MAD2-like OS=Cucurbita moschata OX=3662 GN=LOC111449324 PE=3 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 3.6e-109
Identity = 206/207 (99.52%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEP VFDVLAYTDKDLAVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPRVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDCDDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of CmaCh18G007210 vs. ExPASy TrEMBL
Match: A0A0A0KPY5 (HORMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G590170 PE=3 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 1.2e-107
Identity = 202/207 (97.58%), Postives = 205/207 (99.03%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKTA+KDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESF RVKKYGLPMLLTQDEG
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFVRVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSK+NNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTW+ESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWMESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDD DDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDDDDE 207

[Close]

BLAST of CmaCh18G007210 vs. NCBI nr
Match: XP_022956917.1 (mitotic spindle checkpoint protein MAD2 [Cucurbita moschata] >XP_022967135.1 mitotic spindle checkpoint protein MAD2 [Cucurbita maxima] >XP_023534396.1 mitotic spindle checkpoint protein MAD2-like [Cucurbita pepo subsp. pepo] >KAG6601576.1 Mitotic spindle checkpoint protein MAD2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 408.7 bits (1049), Expect = 3.0e-110
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDCDDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of CmaCh18G007210 vs. NCBI nr
Match: XP_023542211.1 (mitotic spindle checkpoint protein MAD2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 407.5 bits (1046), Expect = 6.8e-110
Identity = 206/207 (99.52%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVV+QGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVDQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDCDDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of CmaCh18G007210 vs. NCBI nr
Match: KAG7012753.1 (Mitotic spindle checkpoint protein MAD2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 407.5 bits (1046), Expect = 6.8e-110
Identity = 206/207 (99.52%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDCDD+
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDD 207

[Close]

BLAST of CmaCh18G007210 vs. NCBI nr
Match: XP_022998654.1 (mitotic spindle checkpoint protein MAD2-like [Cucurbita maxima])

HSP 1 Score: 406.0 bits (1042), Expect = 2.0e-109
Identity = 206/207 (99.52%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDG VVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGVVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDCDDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of CmaCh18G007210 vs. NCBI nr
Match: KAG6573668.1 (Mitotic spindle checkpoint protein MAD2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 404.1 bits (1037), Expect = 7.5e-109
Identity = 206/207 (99.52%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60
           MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEG 60

Query: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120
           VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS
Sbjct: 61  VKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKS 120

Query: 121 DKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180
           DKEIMREIQAIMRQIASSITYLPCLDEP VFDVLAYTDKDLAVPFTWIESDPKLIANPQM
Sbjct: 121 DKEIMREIQAIMRQIASSITYLPCLDEPRVFDVLAYTDKDLAVPFTWIESDPKLIANPQM 180

Query: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           VKLHSFDTKIHKVDTLVSYKNDDCDDE
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKNDDCDDE 207

[Close]

BLAST of CmaCh18G007210 vs. TAIR 10
Match: AT3G25980.1 (DNA-binding HORMA family protein )

HSP 1 Score: 355.1 bits (910), Expect = 3.7e-98
Identity = 179/208 (86.06%), Postives = 194/208 (93.27%), Query Frame = 0


Query: 1   MASKT-ASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDE 60
           MASKT A+KDIITL GSAAIVSEFF YAANSILYNR +YPEESF +VKKYGLPMLL +DE
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFCYAANSILYNRAVYPEESFVKVKKYGLPMLLIEDE 60

Query: 61  GVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREK 120
            VK+F+SNLT+Q+SEWLEAGKLQRVVLVIMSKA  EVLERWNFRIETD EVV++GVSREK
Sbjct: 61  SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQ 180
           SDKEIMREIQAIMRQ+ASS+TYLPCLDE CVFDVLAYTD D+AVPFTWIESDPKLIANPQ
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQ 180

Query: 181 MVKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           MVKLH FDTKIHKVDTLVSYKND+ D+E
Sbjct: 181 MVKLHGFDTKIHKVDTLVSYKNDEWDEE 208

[Close]

BLAST of CmaCh18G007210 vs. TAIR 10
Match: AT3G25980.2 (DNA-binding HORMA family protein )

HSP 1 Score: 340.1 bits (871), Expect = 1.2e-93
Identity = 174/208 (83.65%), Postives = 190/208 (91.35%), Query Frame = 0


Query: 1   MASKT-ASKDIITLRGSAAIVSEFFGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDE 60
           MASKT A+KDIITL GSAAIVSEFF     +ILYNR +YPEESF +VKKYGLPMLL +DE
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFF----CTILYNRAVYPEESFVKVKKYGLPMLLIEDE 60

Query: 61  GVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREK 120
            VK+F+SNLT+Q+SEWLEAGKLQRVVLVIMSKA  EVLERWNFRIETD EVV++GVSREK
Sbjct: 61  SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120

Query: 121 SDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQ 180
           SDKEIMREIQAIMRQ+ASS+TYLPCLDE CVFDVLAYTD D+AVPFTWIESDPKLIANPQ
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQ 180

Query: 181 MVKLHSFDTKIHKVDTLVSYKNDDCDDE 208
           MVKLH FDTKIHKVDTLVSYKND+ D+E
Sbjct: 181 MVKLHGFDTKIHKVDTLVSYKNDEWDEE 204

The following BLAST results are available for this feature:

BLAST of CmaCh18G007210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LU93	5.2e-97	86.06	Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana OX=3702 GN=MAD2 ...	[more]
Q9XFH3	7.1e-94	83.57	Mitotic spindle checkpoint protein MAD2 OS=Zea mays OX=4577 GN=MAD2 PE=2 SV=1	[more]
Q556Y9	6.9e-57	55.72	Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum OX...	[more]
Q9Z1B5	2.3e-52	51.50	Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus OX=10090 GN=Ma...	[more]
Q13257	6.3e-50	49.00	Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens OX=9606 GN=MAD...	[more]

BLAST of CmaCh18G007210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HTK5	1.5e-110	100.00	mitotic spindle checkpoint protein MAD2 OS=Cucurbita maxima OX=3661 GN=LOC111466...	[more]
A0A6J1GXP9	1.5e-110	100.00	mitotic spindle checkpoint protein MAD2 OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KEW7	9.5e-110	99.52	mitotic spindle checkpoint protein MAD2-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1FZG6	3.6e-109	99.52	mitotic spindle checkpoint protein MAD2-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A0A0KPY5	1.2e-107	97.58	HORMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G590170 PE=3 ...	[more]

BLAST of CmaCh18G007210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022956917.1	3.0e-110	100.00	mitotic spindle checkpoint protein MAD2 [Cucurbita moschata] >XP_022967135.1 mit...	[more]
XP_023542211.1	6.8e-110	99.52	mitotic spindle checkpoint protein MAD2 [Cucurbita pepo subsp. pepo]	[more]
KAG7012753.1	6.8e-110	99.52	Mitotic spindle checkpoint protein MAD2 [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_022998654.1	2.0e-109	99.52	mitotic spindle checkpoint protein MAD2-like [Cucurbita maxima]	[more]
KAG6573668.1	7.5e-109	99.52	Mitotic spindle checkpoint protein MAD2, partial [Cucurbita argyrosperma subsp. ...	[more]

BLAST of CmaCh18G007210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25980.1	3.7e-98	86.06	DNA-binding HORMA family protein	[more]
AT3G25980.2	1.2e-93	83.65	DNA-binding HORMA family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036570	HORMA domain superfamily	GENE3D	3.30.900.10	HORMA domain	coord: 1..204 e-value: 1.5E-78 score: 264.8
IPR036570	HORMA domain superfamily	SUPERFAMILY	56019	The spindle assembly checkpoint protein mad2	coord: 10..204
IPR003511	HORMA domain	PFAM	PF02301	HORMA	coord: 16..191 e-value: 2.3E-20 score: 73.1
IPR003511	HORMA domain	PROSITE	PS50815	HORMA	coord: 14..197 score: 45.824375
IPR027097	Mitotic spindle checkpoint protein Mad2	PANTHER	PTHR11842:SF11	MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A	coord: 1..203
IPR045091	Mad2-like	PANTHER	PTHR11842	MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2	coord: 1..203

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh18G007210.1	CmaCh18G007210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0007094	mitotic spindle assembly checkpoint signaling
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000776	kinetochore
cellular_component	GO:0005654	nucleoplasm
molecular_function	GO:0003677	DNA binding