CmaCh18G006640 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh18G006640
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pectin lyase-like superfamily protein
Location	Cma_Chr18: 6093565 .. 6097943 (-)
RNA-Seq Expression	CmaCh18G006640
Synteny	CmaCh18G006640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGTGATCAGAATCGGAAGAACATTCAACCATATCGCACTTTTTTTTTTTTCTTTTTCTTTTTGGTTTCCGATCCGTCTGAGTTTTCCGGTGGTCCATCTCCGCCTTGGTTTCCCGTCGGAAGAGGAGGGAGAAGAAGCAGTTGGATGCGGTGAAGCAACAGGCAGGGCAGGACCTACTGCTTAATATTATCCAGTAGCAACGGTCAGCGTCAATGATTTGTGTGGGTGGTGGTGCCTCAAGCCTCATTATGATCCACCTAATCACATTTATAACTGCTGGATCTTGATCTTCCTCAACAAGCTCCCCTCATTTCATTTTCATTTTCATCTGAAAAGAGATTTGGACGTTTGGAGACCAGGAGCCCGGACGGTGTTGTGATTTGTCATCCATCTGATCAACCGGAGCAACTCAGTCGTATCGTATGAAGACACCTCTCACTGTGAGTTGTTTCCTCTTTCTCAACTCCAACACTTGTAAAATTGACACCAATTCCTACTAATTTCTTCAATTTGATGGTCTGAGGTTTCGCTTTTAAGCTACATTTTGGCTAAACCTCAAAATCCCGATTAATGCTTAATGCCTAGCGCAATTCTCTCTCCGTTGTGGCTGTTGCTACCTTAATCTTAGGTTTTCTTACATTGTTTGTAAAATTGGGGCTAGTGGACAGCAAATTTTCTGATTCCCGTTTTTCTCTTGTGATTTATTTAATTGAAGTTCTGCAAGCTCAAACCAATTACTTTTACTTGATCGCCTCACTTCGATCTATCTTGATTGATATTCAACTGTTTTTCTTTGCCACCAAATTCCTTCGGGTTCTTTGCACCTTTGTAGAAGAAGATCCCTCTGGTAACTTTGTTCCTTGTTCATTGTTAGAGCAAGAAGAACAATGTTAATTGCGGAACTAAGCCAGATGATGCACTTGATCCCTTCCCTTTTGATACTTTTAACAATAGTGGCTTTATTCTTTCAGCTGGTGGATGTTCTTCTGTTGCTTGCATTGTTTTGTATTTCTCCATGGGTTGCTGGGAGTAGCAGCTCTTATTGCAAGCTGACTAATACAGGAGAAATTCGACCCCACAGTGTCTCGATCACTGATTTTGGGGCAGTTGGAGATGGTGTCACTCTCAACACAAAGGCATTCCAAAATGCTATTTTCTACCTCAACTCTTTTGCTGACAAGGGTGGTGCCAAGCTTTTTGTCCCAGCTGGGCGTTGGTTGACAGGAAGCTTCGATCTTATCAGTCATTTAACTTTGTGGTTAGATAAGGATGCTGTGATTCTTGGCTCAACGGTAAGCTCTTGCTTCTTGGTTGAATAAAAGTGTCTACCAAGCCGAGTTTTGAGTTAACTGGTTGTTAGTACACCAAAATTCTCCCCTTGCAAGTGCTTTTTTAATGAACTTGAGCTAATTTTCAATCAGATTAAAGTACAAGATGTAGTGGCATTGTATTCTACACCAAAATTCTCCTCTTGAAAGTGCATTTTTAATGAATTTGAGCTGATTTTCAATCAGATTGAAGTACAAGATGTAGTGGCATTGTATTATCTAATGACGAATGTTTCGTAATTTCTTCAGATCTCTGATGATTGGCCGATTATCAAACCACTGCCATCATATGGTAGAGGACGCGAGTTGCCAGGTGAACGGCATCGAAGCCTCATCTATGGATGCAATTTGACGAATGTTGTCATCACAGGTAGCCCATATTGAAGAACAGTTGTATTCTGCAGTAACCTTTATACTCCTTGATTGTTGTTACTTTTAATTATGTGCAGGTGAAAATGGAACCATTGATGGACAGGGTAGCATCTGGTGGGACTGGTTCCATAGTAAAACGTTAAACTATACGAGGCCGCATTTAGTTGAAATTATGAATTCAACTAATGTTGTAATCTCAAATCTGACATTCAAGAATTCACCCTTTTGGACCCTCCATCCTGTATATTGCAGGTCTTTCTCCATCTGGGGAAGCATAATGTTCATTCTTTCCTTGATCGTTGAACTCGATACTTTATCTATTACGAAGACATGAAACTGAGCTCTTCATTTTTCTATTTTGAATAAACAGCAAAGTCCTTGTCCAGAATGTGACAATCCTTGCACCATCTGATTCTCCAAACACCGATGGTATTGATCCAGGTGAGGCATCATGTTCTTGATACTAACCTTTTATAATCACTTTATTTATTTAGAGCATTCTTTAGTTCAAAAGTATGACTCTGCAAATATGTAGTGAATAGAGAAAAGGTTTCATATATATAGAATATTCGTGAGATCCCACATTGATTGAGAGGAGAACGAAGCATTCTTTATAATGGTGTTGAAACCTCTCCCTAGAAAACGCGTTAGAATATTGTTTGAGCCTTGAAGAATAAGGTAATTCCCAGAAAGCTAAAATTTTGAATATGATGTGCTTCAAGTTAATTATAGTCAGTGCCCCTGCCTTTTAACCGTCTGTTTGAAGCTGACATTGTTATCTTACACAAACTTCTCATCTCCAGATTCTTCATCTGAAGTTTGCATCGAAGATTGCTACATTAGCACAGGTGACGATCTAATTTCGATCAAGAGTGGGTGGGATGAATATGGCGTTGAGTATGCTCGTCCTAGTACAAACATTACCATTCGAAGGCTCACTGGGCAAACTACTAGTGCGGGAATTGCGATTGGAAGTGAGATGTCTGGAGGCGTGTCAGAAATTCTTGTAGAAAACATCCTATTTTTCAACTCGAACATGGGTATCAGAATTAAGACGTCTCCAGGAAGGGGTGGTTACGTGCGCAATGTTTACATATCGAATGTGAGCTTGATCGATGTAAATGTAGCTATAAGGTTCACTGGTAAGTATGGTGAGCACCCAGATTCTGGTTATAATCCAAATGCTCTTCCGACAGTTGAAAGGATCACAATTAAACATGTTAGTGGGGAGAATATTAAAACAGCAGGCGTTTTAGAGGGCATAGAAGGAGATGATTTTCATAATATTTGCTTAGCCAACATCACGCTTAATGTGAGCTCAATGCACCAATGGAACTGCTCGCATGTGCAAGGATTTTCTGACTTTGTTTCTCCAAAGACATGCCATTCTCTGAGGGAAAGAATTTTTCCCCAGCATATTTCGGATTGTTATCTGCCACCAAATTTCATTGCTCAGGTATATTCTCTGAGCTTTGTTGGGTTTAGGCTTTGTTGTCATAATTCTTTCACTCATTTTCGAATATTCGAGTGATTGTATGATTTTGTCTAGTCTCTTGTGTGATAGATGAGTTCAACATGGACCGACTTCATCGGGATGTCGATTGCTTTGCCTGATGAATCAAGTAAAAGCTAAGAGCTGTGTGGCTGAACACTTTCAAATGGCAGGTACACAAACCAAATGTTATTTGTTTTCTGGACTTGGTGTGCAGCATAGTTAATAATGGAGTGATTAATTAGAAATATGTATATAGAGAGAGTTTCTTTTCACACACATTTGCAACCACACTTAGAATACACAAAAGTTAGTGTGGGTGTAATGTATGAACTGAGTTTCTTAAATGATAGCTGAGCAAGATTTCTTTGGGAAGAAGAATTCAGTAATAGCTAATCATACATGGTCGGGGTCGGGTCGGGTCGCACTATGTTCAGAACGGTTGAAAGAACACTATCTCGGGGAGACCATACTCTCCTCCTTGATGATGGAGCATAAAACCTGTCTTGAAATGTAAGTCTCGAGCTCTTTGGACACTTCTGTCAACCTGAGGTCAAAATTGCTTCTGTACTTCCTGTGATTTGGAGATATTGATGCTGTAGTGTCTGGGGCTGGAACTGTGGTCAGAGAGCCCGAGCATCGCTTCCTTGGGCTCGAAAAACTTCCAGGGGAACCAGTAGGGGCCCTTCCCTTAGCCTTAGGCAGCAACTCACCTTTTGCATTCCCCCAACTTCCCCTTCCATCTATATTATCACAATTACTAGTATTAGTATTGTTCTGTAGGCCAGATTTCGTCTCTAGTGGCAGAGGCTTCAGGTAGTCGTGATTGGATATCTGAAGTCTTTCCTTAGCTCTCTCAGCTGCTGCAAGTGCCTTGTCTGTGCTCAGAGTTATCAGTTTCCATGGATCGATACTGATACGAAGGTCTTGTTTCACGGTTGATGTACTCTGGGGATTTGCATCTATTTTTATGGATAATTGTGTCGAGTCCTGAACGTACAACACGAGATTAAGGAAGATCCTCCATAAAACTACGTATCAGTATGTAGCTTACAAAGTATAATTACCTCTTTGACTCGTCCTCCTTGGCACGTGATAAAACGTGACACAAAAGTTGGCTTCTTCTCAAGCAAATCAAAATCATCATCAGAAGAGAAATCCGAGTTTTCGTTGAATGAATCGTCG

mRNA sequence

GAAGTGATCAGAATCGGAAGAACATTCAACCATATCGCACTTTTTTTTTTTTCTTTTTCTTTTTGGTTTCCGATCCGTCTGAGTTTTCCGGTGGTCCATCTCCGCCTTGGTTTCCCGTCGGAAGAGGAGGGAGAAGAAGCAGTTGGATGCGGTGAAGCAACAGGCAGGGCAGGACCTACTGCTTAATATTATCCAGTAGCAACGGTCAGCGTCAATGATTTGTGTGGGTGGTGGTGCCTCAAGCCTCATTATGATCCACCTAATCACATTTATAACTGCTGGATCTTGATCTTCCTCAACAAGCTCCCCTCATTTCATTTTCATTTTCATCTGAAAAGAGATTTGGACGTTTGGAGACCAGGAGCCCGGACGGTGTTGTGATTTGTCATCCATCTGATCAACCGGAGCAACTCAGTCGTATCGTATGAAGACACCTCTCACTCTGGTGGATGTTCTTCTGTTGCTTGCATTGTTTTGTATTTCTCCATGGGTTGCTGGGAGTAGCAGCTCTTATTGCAAGCTGACTAATACAGGAGAAATTCGACCCCACAGTGTCTCGATCACTGATTTTGGGGCAGTTGGAGATGGTGTCACTCTCAACACAAAGGCATTCCAAAATGCTATTTTCTACCTCAACTCTTTTGCTGACAAGGGTGGTGCCAAGCTTTTTGTCCCAGCTGGGCGTTGGTTGACAGGAAGCTTCGATCTTATCAGTCATTTAACTTTGTGGTTAGATAAGGATGCTGTGATTCTTGGCTCAACGATCTCTGATGATTGGCCGATTATCAAACCACTGCCATCATATGGTAGAGGACGCGAGTTGCCAGGTGAACGGCATCGAAGCCTCATCTATGGATGCAATTTGACGAATGTTGTCATCACAGGTGAAAATGGAACCATTGATGGACAGGGTAGCATCTGGTGGGACTGGTTCCATAGTAAAACGTTAAACTATACGAGGCCGCATTTAGTTGAAATTATGAATTCAACTAATGTTGTAATCTCAAATCTGACATTCAAGAATTCACCCTTTTGGACCCTCCATCCTGTATATTGCAGCAAAGTCCTTGTCCAGAATGTGACAATCCTTGCACCATCTGATTCTCCAAACACCGATGGTATTGATCCAGATTCTTCATCTGAAGTTTGCATCGAAGATTGCTACATTAGCACAGGTGACGATCTAATTTCGATCAAGAGTGGGTGGGATGAATATGGCGTTGAGTATGCTCGTCCTAGTACAAACATTACCATTCGAAGGCTCACTGGGCAAACTACTAGTGCGGGAATTGCGATTGGAAGTGAGATGTCTGGAGGCGTGTCAGAAATTCTTGTAGAAAACATCCTATTTTTCAACTCGAACATGGGTATCAGAATTAAGACGTCTCCAGGAAGGGGTGGTTACGTGCGCAATGTTTACATATCGAATGTGAGCTTGATCGATGTAAATGTAGCTATAAGGTTCACTGGTAAGTATGGTGAGCACCCAGATTCTGGTTATAATCCAAATGCTCTTCCGACAGTTGAAAGGATCACAATTAAACATGTTAGTGGGGAGAATATTAAAACAGCAGGCGTTTTAGAGGGCATAGAAGGAGATGATTTTCATAATATTTGCTTAGCCAACATCACGCTTAATGTGAGCTCAATGCACCAATGGAACTGCTCGCATGTGCAAGGATTTTCTGACTTTGTTTCTCCAAAGACATGCCATTCTCTGAGGGAAAGAATTTTTCCCCAGCATATTTCGGATTGTTATCTGCCACCAAATTTCATTGCTCAGATGAGTTCAACATGGACCGACTTCATCGGGATGTCGATTGCTTTGCCTGATGAATCAAGTAAAAGCTAAGAGCTGTGTGGCTGAACACTTTCAAATGGCAGGTACACAAACCAAATGTTATTTGTTTTCTGGACTTGGTGTGCAGCATAGTTAATAATGGAGTGATTAATTAGAAATATGTATATAGAGAGAGTTTCTTTTCACACACATTTGCAACCACACTTAGAATACACAAAAGTTAGTGTGGGTGTAATGTATGAACTGAGTTTCTTAAATGATAGCTGAGCAAGATTTCTTTGGGAAGAAGAATTCAGTAATAGCTAATCATACATGGTCGGGGTCGGGTCGGGTCGCACTATGTTCAGAACGGTTGAAAGAACACTATCTCGGGGAGACCATACTCTCCTCCTTGATGATGGAGCATAAAACCTGTCTTGAAATGTAAGTCTCGAGCTCTTTGGACACTTCTGTCAACCTGAGGTCAAAATTGCTTCTGTACTTCCTGTGATTTGGAGATATTGATGCTGTAGTGTCTGGGGCTGGAACTGTGGTCAGAGAGCCCGAGCATCGCTTCCTTGGGCTCGAAAAACTTCCAGGGGAACCAGTAGGGGCCCTTCCCTTAGCCTTAGGCAGCAACTCACCTTTTGCATTCCCCCAACTTCCCCTTCCATCTATATTATCACAATTACTAGTATTAGTATTGTTCTGTAGGCCAGATTTCGTCTCTAGTGGCAGAGGCTTCAGGTAGTCGTGATTGGATATCTGAAGTCTTTCCTTAGCTCTCTCAGCTGCTGCAAGTGCCTTGTCTGTGCTCAGAGTTATCAGTTTCCATGGATCGATACTGATACGAAGGTCTTGTTTCACGGTTGATGTACTCTGGGGATTTGCATCTATTTTTATGGATAATTGTGTCGAGTCCTGAACGTACAACACGAGATTAAGGAAGATCCTCCATAAAACTACGTATCAGTATGTAGCTTACAAAGTATAATTACCTCTTTGACTCGTCCTCCTTGGCACGTGATAAAACGTGACACAAAAGTTGGCTTCTTCTCAAGCAAATCAAAATCATCATCAGAAGAGAAATCCGAGTTTTCGTTGAATGAATCGTCG

Coding sequence (CDS)

ATGAAGACACCTCTCACTCTGGTGGATGTTCTTCTGTTGCTTGCATTGTTTTGTATTTCTCCATGGGTTGCTGGGAGTAGCAGCTCTTATTGCAAGCTGACTAATACAGGAGAAATTCGACCCCACAGTGTCTCGATCACTGATTTTGGGGCAGTTGGAGATGGTGTCACTCTCAACACAAAGGCATTCCAAAATGCTATTTTCTACCTCAACTCTTTTGCTGACAAGGGTGGTGCCAAGCTTTTTGTCCCAGCTGGGCGTTGGTTGACAGGAAGCTTCGATCTTATCAGTCATTTAACTTTGTGGTTAGATAAGGATGCTGTGATTCTTGGCTCAACGATCTCTGATGATTGGCCGATTATCAAACCACTGCCATCATATGGTAGAGGACGCGAGTTGCCAGGTGAACGGCATCGAAGCCTCATCTATGGATGCAATTTGACGAATGTTGTCATCACAGGTGAAAATGGAACCATTGATGGACAGGGTAGCATCTGGTGGGACTGGTTCCATAGTAAAACGTTAAACTATACGAGGCCGCATTTAGTTGAAATTATGAATTCAACTAATGTTGTAATCTCAAATCTGACATTCAAGAATTCACCCTTTTGGACCCTCCATCCTGTATATTGCAGCAAAGTCCTTGTCCAGAATGTGACAATCCTTGCACCATCTGATTCTCCAAACACCGATGGTATTGATCCAGATTCTTCATCTGAAGTTTGCATCGAAGATTGCTACATTAGCACAGGTGACGATCTAATTTCGATCAAGAGTGGGTGGGATGAATATGGCGTTGAGTATGCTCGTCCTAGTACAAACATTACCATTCGAAGGCTCACTGGGCAAACTACTAGTGCGGGAATTGCGATTGGAAGTGAGATGTCTGGAGGCGTGTCAGAAATTCTTGTAGAAAACATCCTATTTTTCAACTCGAACATGGGTATCAGAATTAAGACGTCTCCAGGAAGGGGTGGTTACGTGCGCAATGTTTACATATCGAATGTGAGCTTGATCGATGTAAATGTAGCTATAAGGTTCACTGGTAAGTATGGTGAGCACCCAGATTCTGGTTATAATCCAAATGCTCTTCCGACAGTTGAAAGGATCACAATTAAACATGTTAGTGGGGAGAATATTAAAACAGCAGGCGTTTTAGAGGGCATAGAAGGAGATGATTTTCATAATATTTGCTTAGCCAACATCACGCTTAATGTGAGCTCAATGCACCAATGGAACTGCTCGCATGTGCAAGGATTTTCTGACTTTGTTTCTCCAAAGACATGCCATTCTCTGAGGGAAAGAATTTTTCCCCAGCATATTTCGGATTGTTATCTGCCACCAAATTTCATTGCTCAGATGAGTTCAACATGGACCGACTTCATCGGGATGTCGATTGCTTTGCCTGATGAATCAAGTAAAAGCTAA

Protein sequence

MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYNPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGFSDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQMSSTWTDFIGMSIALPDESSKS

Homology

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BLAST of CmaCh18G006640 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 3.8e-127
Identity = 212/412 (51.46%), Postives = 287/412 (69.66%), Query Frame = 0


Query: 40  RPHSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHL 99
           R HS S+ DFG VGDG TLNTKAFQ+A+  L+ +  +GGA+L+VPAG+WLTGSF L SH 
Sbjct: 59  RAHSASLVDFGGVGDGQTLNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHF 118

Query: 100 TLWLDKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTI 159
           TL+L +DAV+L S     WP+IKPLPSYGRGR+    R+ SLI+G NLT+V+ITG+NGTI
Sbjct: 119 TLFLHRDAVLLASQDISQWPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTI 178

Query: 160 DGQGSIWWDWFHSKTLNYTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNV 219
           DGQG +WW  FH   L YTRP+L+E+M S ++ ISNLT  NSP W +HPVY   +L+Q +
Sbjct: 179 DGQGGLWWQRFHGGKLKYTRPYLIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGI 238

Query: 220 TILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRR 279
           TILAP  SPNTDGI+PDS +   IEDCYI +GDD +++KSGWDEYG+ Y  P+  + IRR
Sbjct: 239 TILAPVRSPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRR 298

Query: 280 LTG-QTTSAGIAIGSEMSGGVSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSL 339
           LT     SA IA+GSEMSGG+ ++  E+I+  NS  GIRIKT  GRGGYV+++Y+  +++
Sbjct: 299 LTCISPYSAVIALGSEMSGGIQDVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTM 358

Query: 340 IDVNVAIRFTGKYGEHPDSGYNPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNIC 399
             +  A   TG YG H D+ Y+P A P ++ I  + +  EN+  A  LEGI  D F  IC
Sbjct: 359 KTMKWAFWMTGNYGSHADNHYDPKAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGIC 418

Query: 400 LANITLNVSSMHQ---WNCSHVQGFSDFVSPKTCHSLRERIFPQHISDCYLP 448
           ++N+T+++++  +   W C+ V+G S  V+P  C +L ++  P+  S C  P
Sbjct: 419 ISNVTIHLAAKAKKVPWTCTDVEGISSGVTPTPCSTLPDQ-GPEKTSLCNFP 469

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BLAST of CmaCh18G006640 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.4e-28
Identity = 116/411 (28.22%), Postives = 185/411 (45.01%), Query Frame = 0


Query: 45  SITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLD 104
           ++  +GAVGDG   +T AF+      N+  +K  A   VPA +       ++++L  +  
Sbjct: 61  NVEHYGAVGDGKHDSTDAFEKT---WNAACNKLSAVFLVPANKKF-----VVNNLVFYGP 120

Query: 105 KDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGS 164
                  S   D      P P+  +  ++        ++   LT+  + G  G IDGQG+
Sbjct: 121 CQPHF--SFKVDGTIAAYPDPAKWKNSKI-------WMHFARLTDFNLMG-TGVIDGQGN 180

Query: 165 IWWDWFHSKTLN-------YTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQ 224
            WW     KT+N         RP  ++I  S +V +  LT  NSP + L    C  V +Q
Sbjct: 181 RWWS-DQCKTINGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQ 240

Query: 225 NVTILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITI 284
            + I AP DSPNTDGID  +S    IE C I TGDD +++ +G           S+NITI
Sbjct: 241 GIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTG-----------SSNITI 300

Query: 285 RRLTGQTTSAGIAIGSEMSGG----VSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYI 344
           + LT      G++IGS   G     VS + ++   F ++  G+RIKT  G  G   ++  
Sbjct: 301 KDLT-CGPGHGMSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITY 360

Query: 345 SNVSLIDVNVAIRFTGKYGEHPDSGYNPNALPTVERITIKHVSGENIKTAGV-LEGIEGD 404
            NV +I+    I     Y     +  N  +   ++ +T K++ G +  TA + L   +  
Sbjct: 361 ENVEMINAENPILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTSATTAAIQLMCSDSV 420

Query: 405 DFHNICLANITLNVSSMHQWNCSHVQGFSDFVSP--KTCHSLRERIFPQHI 442
              NI L+N+ L ++S     C +      + +P   +C SL     P+ +
Sbjct: 421 PCSNIKLSNVFLKLTSGKVATCVNKNANGYYTNPLNPSCKSLHPGRTPKEL 440

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BLAST of CmaCh18G006640 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 4.1e-28
Identity = 123/426 (28.87%), Postives = 187/426 (43.90%), Query Frame = 0


Query: 26  SSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPA 85
           S+ S  KL ++        ++  +GAVGDG   +T+AF       N+   K  A L VPA
Sbjct: 41  SNRSLKKLVHSRHDAATVFNVEQYGAVGDGKHDSTEAFATT---WNAACKKASAVLLVPA 100

Query: 86  GRW-----LTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRS 145
            +      L        HL+  +D        TI     + +P P+  +  ++       
Sbjct: 101 NKKFFVNNLVFRGPCQPHLSFKVD-------GTI-----VAQPDPARWKNSKI------- 160

Query: 146 LIYGCNLTNVVITGENGTIDGQGSIWW----DWFHSKTL--NYTRPHLVEIMNSTNVVIS 205
            +    LT+  + G  G IDGQG  WW       + +T+  +  RP  ++I  S +V + 
Sbjct: 161 WLQFAQLTDFNLMG-TGVIDGQGQQWWAGQCKVVNGRTVCNDRNRPTAIKIDYSKSVTVK 220

Query: 206 NLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDL 265
            LT  NSP + L    C  V +Q + I AP DSPNTDGID  +S    IE C I TGDD 
Sbjct: 221 ELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDC 280

Query: 266 ISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGS----EMSGGVSEILVENILFF 325
           I+I +G           S+NITI+ L       GI+IGS         VS + V    F 
Sbjct: 281 IAIGTG-----------SSNITIKDLI-CGPGHGISIGSLGRDNSRAEVSHVHVNRAKFI 340

Query: 326 NSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYNPNALPTVERI 385
           ++  G+RIKT  G  G    +   NV +I+    I     Y     +  N  +   ++ +
Sbjct: 341 DTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSASACQNQRSAVQIQGV 400

Query: 386 TIKHVSGENIKTAGV-LEGIEGDDFHNICLANITLNVSSMHQWNC--SHVQGFSDFVSPK 434
           T K++ G +   A + L   +      I L+N++L ++S    +C   + +GF       
Sbjct: 401 TYKNIHGTSATAAAIQLMCSDSVPCTGIQLSNVSLKLTSGKPASCVDKNARGFYSGRLIP 431

[Close]

BLAST of CmaCh18G006640 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 4.1e-28
Identity = 118/403 (29.28%), Postives = 188/403 (46.65%), Query Frame = 0


Query: 42  HSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTL 101
           +++ +T FGAVGDGVT +++AF  A  +    +  G  +  VPAG            +T 
Sbjct: 22  NALDVTQFGAVGDGVTDDSQAFLKA--WEAVCSGTGDGQFVVPAG------------MTF 81

Query: 102 WLDKDAVILGSTISDDWPIIKPL------PSYGRGRELPGERHRSLIYGCNLTNVVITGE 161
            L +     GS  S   P+   +      PS G  +   G++ + +++  ++  +VI G+
Sbjct: 82  ML-QPLKFQGSCKST--PVFVQMLGKLVAPSKGNWK---GDKDQWILF-TDIEGLVIEGD 141

Query: 162 NGTIDGQGSIWWDWFHSKTLNYTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVL 221
            G I+GQGS WW+   S      RP  ++  +  N+ +S LT  +SP   +H   C+ V 
Sbjct: 142 -GEINGQGSSWWEHKGS------RPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVT 201

Query: 222 VQNVTILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNI 281
           + ++ I AP  SPNTDGID  +SS V I+DC I+TGDD I+I SG           ++NI
Sbjct: 202 ISSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TSNI 261

Query: 282 TIRRLTGQTTSAGIAIGSEMSGG----VSEILVENILFFNSNMGIRIKTSPGRGGYVRNV 341
            I  +       GI+IGS    G    V  + V+N  F  +  G RIKT  G  GY R +
Sbjct: 262 HISGI-DCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMI 321

Query: 342 YISNVSLIDVNVAIRFTGKY--GEHPDSGYNPNALPTVERITIKHVSGENIKTAGV-LEG 401
             + ++L +V   I     Y  G+  ++    ++   V ++   +  G +    GV    
Sbjct: 322 TFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRC 381

Query: 402 IEGDDFHNICLANITLNVSS-----MHQWNCSHVQGFSDFVSP 427
            E      I L ++ +  +S     + Q  C +V+G S    P
Sbjct: 382 SERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVP 384

[Close]

BLAST of CmaCh18G006640 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 119.0 bits (297), Expect = 1.4e-25
Identity = 97/346 (28.03%), Postives = 149/346 (43.06%), Query Frame = 0


Query: 41  PHSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGR-WLTGSFDLIS-- 100
           P +VS++DFGA GDG T +T+AF NA  +  + +  G   L VP G  +L  S  L    
Sbjct: 65  PTTVSVSDFGAKGDGKTDDTQAFVNA--WKKACSSNGAVNLLVPKGNTYLLKSIQLTGPC 124

Query: 101 HLTLWLDKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENG 160
           +  L +     +  S    D+  I     +     L                 V  G+ G
Sbjct: 125 NSILTVQIFGTLSASQKRSDYKDISKWIMFDGVNNLS----------------VDGGDTG 184

Query: 161 TIDGQGSIWW--DWFHSKTLNYTR-PHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKV 220
            +DG G  WW      +K    T+ P  +   NS ++++ NL  +N+    +    CS V
Sbjct: 185 VVDGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNV 244

Query: 221 LVQNVTILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTN 280
            V NV + AP+DSPNTDGI   ++  + + +  I TGDD ISI+SG           S N
Sbjct: 245 QVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------SQN 304

Query: 281 ITIRRLT---GQTTSAGIAIGSEMSGGVSEILVENILFFNSNMGIRIKTSPGRGGYVRNV 340
           + I  +T   G   S G          VS + V+      ++ G+RIKT  G  G   N+
Sbjct: 305 VQINDITCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNI 364

Query: 341 YISNVSLIDVNVAIRFTGKYGEHPDSGYNPNALPTVERITIKHVSG 378
              N+ + +V   I     Y +        +A+  V+ +  + +SG
Sbjct: 365 IFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAV-QVKNVVYRDISG 380

[Close]

BLAST of CmaCh18G006640 vs. ExPASy TrEMBL
Match: A0A6J1HR20 (probable polygalacturonase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466589 PE=3 SV=1)

HSP 1 Score: 983.4 bits (2541), Expect = 3.3e-283
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
           MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT
Sbjct: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60

Query: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
           KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI
Sbjct: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120

Query: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
           IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP
Sbjct: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180

Query: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
           HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE
Sbjct: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240

Query: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300
           VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300

Query: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360
           EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN
Sbjct: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360

Query: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420
           PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF
Sbjct: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420

Query: 421 SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQMSSTWTDFIGMSIALPDESSKS 476
           SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQMSSTWTDFIGMSIALPDESSKS
Sbjct: 421 SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQMSSTWTDFIGMSIALPDESSKS 475

[Close]

BLAST of CmaCh18G006640 vs. ExPASy TrEMBL
Match: A0A6J1G153 (probable polygalacturonase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449733 PE=3 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 1.1e-281
Identity = 473/475 (99.58%), Postives = 473/475 (99.58%), Query Frame = 0


Query: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
           MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT
Sbjct: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60

Query: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
           KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI
Sbjct: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120

Query: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
           IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP
Sbjct: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180

Query: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
           HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE
Sbjct: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240

Query: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300
           VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300

Query: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360
           EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDS YN
Sbjct: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYN 360

Query: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420
           PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF
Sbjct: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420

Query: 421 SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQMSSTWTDFIGMSIALPDESSKS 476
           SDFVSPKTCHSLRERIFPQHISDCY PPNFIAQMSSTWTDFIGMSIALPDESSKS
Sbjct: 421 SDFVSPKTCHSLRERIFPQHISDCYQPPNFIAQMSSTWTDFIGMSIALPDESSKS 475

[Close]

BLAST of CmaCh18G006640 vs. ExPASy TrEMBL
Match: A0A6J1HPS3 (probable polygalacturonase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466589 PE=3 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 3.2e-270
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0


Query: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
           MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT
Sbjct: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60

Query: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
           KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI
Sbjct: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120

Query: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
           IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP
Sbjct: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180

Query: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
           HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE
Sbjct: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240

Query: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300
           VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300

Query: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360
           EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN
Sbjct: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360

Query: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420
           PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF
Sbjct: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420

Query: 421 SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQ 454
           SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQ
Sbjct: 421 SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQ 453

[Close]

BLAST of CmaCh18G006640 vs. ExPASy TrEMBL
Match: A0A6J1G174 (probable polygalacturonase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449733 PE=3 SV=1)

HSP 1 Score: 935.3 bits (2416), Expect = 1.0e-268
Identity = 451/453 (99.56%), Postives = 451/453 (99.56%), Query Frame = 0


Query: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
           MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT
Sbjct: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60

Query: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
           KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI
Sbjct: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120

Query: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
           IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP
Sbjct: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180

Query: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
           HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE
Sbjct: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240

Query: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300
           VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300

Query: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360
           EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDS YN
Sbjct: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYN 360

Query: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420
           PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF
Sbjct: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420

Query: 421 SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQ 454
           SDFVSPKTCHSLRERIFPQHISDCY PPNFIAQ
Sbjct: 421 SDFVSPKTCHSLRERIFPQHISDCYQPPNFIAQ 453

[Close]

BLAST of CmaCh18G006640 vs. ExPASy TrEMBL
Match: A0A6J1DDC5 (probable polygalacturonase isoform X3 OS=Momordica charantia OX=3673 GN=LOC111019738 PE=3 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 8.8e-236
Identity = 392/452 (86.73%), Postives = 424/452 (93.81%), Query Frame = 0


Query: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
           MKTP TLVDVLLLLALFC++P +A  SSS+CKL N  EIRPHSVSITDFGAVGDG+TLNT
Sbjct: 1   MKTPFTLVDVLLLLALFCLAPRIA-RSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNT 60

Query: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
           KAFQNAIFYLNSFADKGGAKL VPAGRWLTGSFDLISHLTLWLDKDAVILGSTIS+DWP+
Sbjct: 61  KAFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPV 120

Query: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
           +KPLPSYGRGRELPG+RHRSLIYGCNLT+V+ITGENGTIDGQGSIWWDWFH KTLNYTRP
Sbjct: 121 VKPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRP 180

Query: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
           HLVE+MNST VVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAP DSPNTDGIDPDSS+E
Sbjct: 181 HLVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAE 240

Query: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQT-TSAGIAIGSEMSGGV 300
           VCIEDCYISTGDDLISIKSGWD+YG+EYA PSTNITIRRLTGQT TSAG+AIGSEMSGG+
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGI 300

Query: 301 SEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGY 360
           SEI VEN+LFFNS MGIRIKTSPGR GYVRNVYISNVSL +V++AIRFTG YGEHPDS Y
Sbjct: 301 SEIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCY 360

Query: 361 NPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQG 420
           +P ALPTVE ITIKHVSGENI+TAG+LEGIEGDDFHNICL+NITLNV+SMH WNCSHVQG
Sbjct: 361 DPMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQG 420

Query: 421 FSDFVSPKTCHSLRERIFPQHISDCYLPPNFI 452
           FSD VSP+ C  L+E+I P+HI +CYLPPNF+
Sbjct: 421 FSDLVSPEICQPLKEKISPRHILECYLPPNFL 451

[Close]

BLAST of CmaCh18G006640 vs. NCBI nr
Match: XP_022967081.1 (probable polygalacturonase isoform X1 [Cucurbita maxima])

HSP 1 Score: 983.4 bits (2541), Expect = 6.8e-283
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
           MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT
Sbjct: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60

Query: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
           KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI
Sbjct: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120

Query: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
           IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP
Sbjct: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180

Query: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
           HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE
Sbjct: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240

Query: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300
           VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300

Query: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360
           EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN
Sbjct: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360

Query: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420
           PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF
Sbjct: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420

Query: 421 SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQMSSTWTDFIGMSIALPDESSKS 476
           SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQMSSTWTDFIGMSIALPDESSKS
Sbjct: 421 SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQMSSTWTDFIGMSIALPDESSKS 475

[Close]

BLAST of CmaCh18G006640 vs. NCBI nr
Match: XP_023542118.1 (probable polygalacturonase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 979.2 bits (2530), Expect = 1.3e-281
Identity = 472/475 (99.37%), Postives = 474/475 (99.79%), Query Frame = 0


Query: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
           MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT
Sbjct: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60

Query: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
           KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI
Sbjct: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120

Query: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
           IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP
Sbjct: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180

Query: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
           HLVE+MNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE
Sbjct: 181 HLVELMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240

Query: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300
           VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300

Query: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360
           EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN
Sbjct: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360

Query: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420
           PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF
Sbjct: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420

Query: 421 SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQMSSTWTDFIGMSIALPDESSKS 476
           SDFVSPKTCHSLRERIFPQHISDCY PPNFIAQMSSTWTDFIGMSIALPDESS+S
Sbjct: 421 SDFVSPKTCHSLRERIFPQHISDCYQPPNFIAQMSSTWTDFIGMSIALPDESSRS 475

[Close]

BLAST of CmaCh18G006640 vs. NCBI nr
Match: XP_022945523.1 (probable polygalacturonase isoform X1 [Cucurbita moschata])

HSP 1 Score: 978.4 bits (2528), Expect = 2.2e-281
Identity = 473/475 (99.58%), Postives = 473/475 (99.58%), Query Frame = 0


Query: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
           MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT
Sbjct: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60

Query: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
           KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI
Sbjct: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120

Query: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
           IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP
Sbjct: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180

Query: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
           HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE
Sbjct: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240

Query: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300
           VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300

Query: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360
           EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDS YN
Sbjct: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYN 360

Query: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420
           PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF
Sbjct: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420

Query: 421 SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQMSSTWTDFIGMSIALPDESSKS 476
           SDFVSPKTCHSLRERIFPQHISDCY PPNFIAQMSSTWTDFIGMSIALPDESSKS
Sbjct: 421 SDFVSPKTCHSLRERIFPQHISDCYQPPNFIAQMSSTWTDFIGMSIALPDESSKS 475

[Close]

BLAST of CmaCh18G006640 vs. NCBI nr
Match: XP_022967082.1 (probable polygalacturonase isoform X2 [Cucurbita maxima])

HSP 1 Score: 940.3 bits (2429), Expect = 6.6e-270
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0


Query: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
           MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT
Sbjct: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60

Query: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
           KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI
Sbjct: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120

Query: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
           IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP
Sbjct: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180

Query: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
           HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE
Sbjct: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240

Query: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300
           VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300

Query: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360
           EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN
Sbjct: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360

Query: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420
           PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF
Sbjct: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420

Query: 421 SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQ 454
           SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQ
Sbjct: 421 SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQ 453

[Close]

BLAST of CmaCh18G006640 vs. NCBI nr
Match: XP_023542119.1 (probable polygalacturonase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 937.2 bits (2421), Expect = 5.6e-269
Identity = 451/453 (99.56%), Postives = 452/453 (99.78%), Query Frame = 0


Query: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
           MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT
Sbjct: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60

Query: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
           KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI
Sbjct: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120

Query: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
           IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP
Sbjct: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180

Query: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
           HLVE+MNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE
Sbjct: 181 HLVELMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240

Query: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300
           VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVS 300

Query: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360
           EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN
Sbjct: 301 EILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYN 360

Query: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420
           PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF
Sbjct: 361 PNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGF 420

Query: 421 SDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQ 454
           SDFVSPKTCHSLRERIFPQHISDCY PPNFIAQ
Sbjct: 421 SDFVSPKTCHSLRERIFPQHISDCYQPPNFIAQ 453

[Close]

BLAST of CmaCh18G006640 vs. TAIR 10
Match: AT3G62110.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 657.1 bits (1694), Expect = 1.0e-188
Identity = 315/451 (69.84%), Postives = 372/451 (82.48%), Query Frame = 0


Query: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEI-RPHSVSITDFGAVGDGVTLN 60
           MK    L+ VLL+ A +    W +   S +C+ +N   + RPHSVSIT+FGAVGDGVTLN
Sbjct: 1   MKRSFLLLYVLLVQAFY--GAWCSVGESLHCEYSNLASLHRPHSVSITEFGAVGDGVTLN 60

Query: 61  TKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWP 120
           TKAFQNA+FYLNSF+DKGGAKLFVPAG+WLTGSFDLISHLTLWLDK A ILGST S++WP
Sbjct: 61  TKAFQNALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISHLTLWLDKGATILGSTSSENWP 120

Query: 121 IIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTR 180
           ++ PLPSYGRGRELPG RHRSLIYG NLT+VVITGENGTIDGQG++WWDWF +  LNYTR
Sbjct: 121 VVDPLPSYGRGRELPGRRHRSLIYGQNLTDVVITGENGTIDGQGTVWWDWFRNGELNYTR 180

Query: 181 PHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSS 240
           PHLVE+MNST ++ISNLTF NSPFW +HPVYC  V+V+N+TILAP +SPNTDG+DPDSS+
Sbjct: 181 PHLVELMNSTGLIISNLTFLNSPFWNIHPVYCRDVVVKNLTILAPLESPNTDGVDPDSST 240

Query: 241 EVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTT-SAGIAIGSEMSGG 300
            VCIEDCYI TGDDL+SIKSGWDEYG+ YARPS+ I I RLTGQTT S+GIAIGSEMSGG
Sbjct: 241 NVCIEDCYIVTGDDLVSIKSGWDEYGISYARPSSKIKINRLTGQTTSSSGIAIGSEMSGG 300

Query: 301 VSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSG 360
           VSEI ++++  FNSN GIRIKTS GRGGYVRNV+I NV L +V  AIRFTGKYGEHPD  
Sbjct: 301 VSEIYIKDLHLFNSNTGIRIKTSAGRGGYVRNVHILNVKLDNVKKAIRFTGKYGEHPDEK 360

Query: 361 YNPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNV---SSMHQWNCS 420
           Y+P ALP +E+IT ++V+G+ I  AG+LEGIEGD F NIC  N+TL V   S    W CS
Sbjct: 361 YDPKALPAIEKITFENVNGDGIGVAGLLEGIEGDVFKNICFLNVTLRVKKNSKKSPWECS 420

Query: 421 HVQGFSDFVSPK-TCHSLRERIFPQHISDCY 446
           +V+G+S +VSP+ TC SL+E IFP+H SDC+
Sbjct: 421 NVRGYSQWVSPEITCDSLKESIFPEHGSDCF 449

[Close]

BLAST of CmaCh18G006640 vs. TAIR 10
Match: AT4G23820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 477.2 bits (1227), Expect = 1.5e-134
Identity = 231/428 (53.97%), Postives = 300/428 (70.09%), Query Frame = 0


Query: 8   VDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNAI 67
           V + L   LF +S    G S + C        R   +SITDFG VGDG T+NTKAF+ AI
Sbjct: 6   VSIFLFSCLF-VSSSSLGDSEATCSGIVPLRYRYDKISITDFGGVGDGRTVNTKAFRAAI 65

Query: 68  FYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPSY 127
           + +     +GG  L++P G +LT SF+L SH+TL+L K AVI     + +WP+I PLPSY
Sbjct: 66  YRIQHLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIRAVQDTWNWPLIDPLPSY 125

Query: 128 GRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIMN 187
           GRGRELPG R+ S I+G  L +VVITG+NGTIDGQG +WW+ + S+TL YTRP+L+E  +
Sbjct: 126 GRGRELPGGRYMSFIHGDGLRDVVITGQNGTIDGQGEVWWNMWRSRTLKYTRPNLIEFKD 185

Query: 188 STNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDCY 247
           S  ++ISN+ F+NSPFW +HPVYCS V++ +VTILAP DSPNTDGIDPDSS  VCIED Y
Sbjct: 186 SKEIIISNVIFQNSPFWNIHPVYCSNVVIHHVTILAPQDSPNTDGIDPDSSYNVCIEDSY 245

Query: 248 ISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVENI 307
           ISTGDDL++IKSGWD+YG+ Y RPS+NITIRR+TG +  AGIAIGSE SGG+  I+ E+I
Sbjct: 246 ISTGDDLVAIKSGWDQYGIAYGRPSSNITIRRITGSSPFAGIAIGSETSGGIKNIIAEHI 305

Query: 308 LFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYNPNALPTV 367
              N  +G+ IKT+ GRGGY++N+ IS+V +      I+  G  G+HPD  YNPNALP V
Sbjct: 306 TLSNMGVGVNIKTNIGRGGYIKNIKISDVYVDTAKYGIKIAGDTGDHPDENYNPNALPVV 365

Query: 368 ERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITL--NVSSMHQWNCSHVQGFSDFVS 427
           + I IK+V G N++ AG ++G++G  F  ICL+ I L  +++S   W CS V G S  VS
Sbjct: 366 KGIHIKNVWGVNVRNAGSIQGLKGSPFTGICLSEINLHGSLNSYKTWKCSDVSGTSLKVS 425

Query: 428 PKTCHSLR 434
           P  C  LR
Sbjct: 426 PWPCSELR 432

[Close]

BLAST of CmaCh18G006640 vs. TAIR 10
Match: AT4G33440.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 471.9 bits (1213), Expect = 6.2e-133
Identity = 223/394 (56.60%), Postives = 295/394 (74.87%), Query Frame = 0


Query: 44  VSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWL 103
           +SITDFG VGDG T NT AF+ A+ +L  FA +GGA+L VP G WL+GSF+L S+ TL+L
Sbjct: 72  MSITDFGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVPEGTWLSGSFNLTSNFTLFL 131

Query: 104 DKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQG 163
           ++ A+ILGS   D+WPII+PLPSYGRGRE PG RH SLI+G NLTNVVITGENGTIDGQG
Sbjct: 132 ERGALILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHGDNLTNVVITGENGTIDGQG 191

Query: 164 SIWWDWFHSKTLNYTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILA 223
            +WW+ + ++TL +TR HL+E+ NS N++ISNLT  NSPFWT+HPVYCS V+++N+TILA
Sbjct: 192 KMWWELWWNRTLVHTRGHLIELKNSHNILISNLTLLNSPFWTIHPVYCSNVVIRNMTILA 251

Query: 224 PSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQ 283
           P ++PNTDGIDPDSS+ VCIEDCYI +GDDL+++KSGWD+YG+  ARPS+NI IRR++G 
Sbjct: 252 PMNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGMAVARPSSNIVIRRISGT 311

Query: 284 T-TSAGIAIGSEMSGGVSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVN 343
           T T +G+ IGSEMSGG+  I VE+I  ++S  G+RIKT  GRGGY+ N+  +NV L  V 
Sbjct: 312 TRTCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTDKGRGGYISNITFNNVLLEKVK 371

Query: 344 VAIRFTGKYGEHPDSGYNPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANI 403
           V IRF+    +H D  ++P ALP V+ I I +V   N + A +L G+EG  F ++CL N+
Sbjct: 372 VPIRFSSGSNDHSDDKWDPKALPRVKGIYISNVVSLNSRKAPMLLGVEGTSFQDVCLRNV 431

Query: 404 T-LNVSSMHQWNCSHVQGFSDFVSPKTCHSLRER 436
           T L +    +W C  V G++  V P +C  L ++
Sbjct: 432 TLLGLPKTEKWKCKDVSGYASDVFPLSCPQLLQK 465

[Close]

BLAST of CmaCh18G006640 vs. TAIR 10
Match: AT3G16850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 461.8 bits (1187), Expect = 6.5e-130
Identity = 230/433 (53.12%), Postives = 302/433 (69.75%), Query Frame = 0


Query: 1   MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
           MK P+ LV  LL   +  IS      +++ CK     + RPHSVSI +FGAVGDG TLNT
Sbjct: 1   MKMPVALV-WLLAFTILLIS---GEGNNAICKENFKLDPRPHSVSILEFGAVGDGKTLNT 60

Query: 61  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
            AFQNA+FYL SFADKGGA+L+VP G+WLTGSF+L SHLTL+L+K A IL S     W +
Sbjct: 61  IAFQNAVFYLKSFADKGGAQLYVPPGKWLTGSFNLTSHLTLFLEKGATILASPDPSHWDV 120

Query: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
           + PLPSYGRG ELPG+R+RSLI G NL +VVITGENGT DGQG+ WW+W  S +LNY+RP
Sbjct: 121 VSPLPSYGRGIELPGKRYRSLINGDNLIDVVITGENGTFDGQGAAWWEWLESGSLNYSRP 180

Query: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
           H++E ++S +++ISNLTF N+P   +HPVYCS++ ++ V I    DSP+  G+ PDSS  
Sbjct: 181 HIIEFVSSKHILISNLTFLNAPAINIHPVYCSQIHIRKVLIETSVDSPHVLGVAPDSSDN 240

Query: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQT-TSAGIAIGSEMSGGV 300
           VCIED  I+ G D +S+KSGWD+YG+ Y RP+T + IR L  ++ T AGI+ GSEMSGGV
Sbjct: 241 VCIEDSTINVGHDAVSLKSGWDQYGIHYGRPTTAVHIRNLRLKSPTGAGISFGSEMSGGV 300

Query: 301 SEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGY 360
           S++ VE +   +S +G+  +T+ GRGGY+RN+ IS+V L  V+ AI   G  G HPD  +
Sbjct: 301 SDVTVERLNIHSSLIGVAFRTTRGRGGYIRNITISDVDLTSVDTAIVANGHTGSHPDDKF 360

Query: 361 NPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQG 420
           + +ALP V  I +++ +G +I  AG L GI    F +ICLA+I L   S   W CS+V G
Sbjct: 361 DRDALPVVTHIVMRNFTGVDIGVAGNLTGIGESPFTSICLADIHLQTRSEESWICSNVSG 420

Query: 421 FSDFVSPKTCHSL 433
           FSD VSP+ C  L
Sbjct: 421 FSDDVSPEPCQEL 429

[Close]

BLAST of CmaCh18G006640 vs. TAIR 10
Match: AT3G61490.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 450.7 bits (1158), Expect = 1.5e-126
Identity = 208/397 (52.39%), Postives = 285/397 (71.79%), Query Frame = 0


Query: 40  RPHSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHL 99
           R HS SIT++G VGDG TLNTKAFQ+A+ +L+ ++ +GGA+LFVPAG+WLTGSF+L SH 
Sbjct: 41  RSHSASITEYGGVGDGKTLNTKAFQSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHF 100

Query: 100 TLWLDKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTI 159
           TL+L KDA++L +   +++PI+K LPSYGRGR+  G R  SLI+G NL++V+ITG NGTI
Sbjct: 101 TLFLHKDAILLAAQDLNEYPILKALPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTI 160

Query: 160 DGQGSIWWDWFHSKTLNYTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNV 219
           DGQGS WW  FH   L YTRP+L+E+M S  + ISNLTF +SP W +HPVY S ++V+ V
Sbjct: 161 DGQGSFWWQKFHGGKLKYTRPYLIELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGV 220

Query: 220 TILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRR 279
           TI+AP  SPNTDGI+PDS +   IEDCYI +GDD I++KSGWDEYG+ +  P+ ++ IRR
Sbjct: 221 TIIAPVKSPNTDGINPDSCTNTRIEDCYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRR 280

Query: 280 LTG-QTTSAGIAIGSEMSGGVSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSL 339
           LT     SA IA+GSEMSGG+ ++  E+I  + +  G+RIKT+ GRG +V+N+Y+  ++L
Sbjct: 281 LTCISPYSAAIALGSEMSGGIEDVRAEDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNL 340

Query: 340 IDVNVAIRFTGKYGEHPDSGYNPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNIC 399
             +      TG Y  H DS Y+P+ALP +  I  + +  EN+  AG LEGI GD F  IC
Sbjct: 341 HTMKWVFWMTGNYKAHADSHYDPHALPEITGINYRDIVAENVSMAGRLEGISGDPFTGIC 400

Query: 400 LANITLNVSSMHQ---WNCSHVQGFSDFVSPKTCHSL 433
           ++N T+++++ H+   W CS V+G +  V PK C  L
Sbjct: 401 ISNATISMAAKHKKAIWMCSDVEGVTSGVDPKPCDLL 437

The following BLAST results are available for this feature:

BLAST of CmaCh18G006640 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7PZL3	3.8e-127	51.46	Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ...	[more]
Q9FY19	2.4e-28	28.22	Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1	[more]
Q7M1E7	4.1e-28	28.87	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
Q9LW07	4.1e-28	29.28	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
Q8RY29	1.4e-25	28.03	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]

BLAST of CmaCh18G006640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HR20	3.3e-283	100.00	probable polygalacturonase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146658...	[more]
A0A6J1G153	1.1e-281	99.58	probable polygalacturonase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449...	[more]
A0A6J1HPS3	3.2e-270	100.00	probable polygalacturonase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11146658...	[more]
A0A6J1G174	1.0e-268	99.56	probable polygalacturonase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449...	[more]
A0A6J1DDC5	8.8e-236	86.73	probable polygalacturonase isoform X3 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]

BLAST of CmaCh18G006640 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022967081.1	6.8e-283	100.00	probable polygalacturonase isoform X1 [Cucurbita maxima]	[more]
XP_023542118.1	1.3e-281	99.37	probable polygalacturonase isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022945523.1	2.2e-281	99.58	probable polygalacturonase isoform X1 [Cucurbita moschata]	[more]
XP_022967082.1	6.6e-270	100.00	probable polygalacturonase isoform X2 [Cucurbita maxima]	[more]
XP_023542119.1	5.6e-269	99.56	probable polygalacturonase isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh18G006640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62110.1	1.0e-188	69.84	Pectin lyase-like superfamily protein	[more]
AT4G23820.1	1.5e-134	53.97	Pectin lyase-like superfamily protein	[more]
AT4G33440.1	6.2e-133	56.60	Pectin lyase-like superfamily protein	[more]
AT3G16850.1	6.5e-130	53.12	Pectin lyase-like superfamily protein	[more]
AT3G61490.1	1.5e-126	52.39	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 299..320 e-value: 560.0 score: 9.0 coord: 272..293 e-value: 530.0 score: 9.2 coord: 211..237 e-value: 600.0 score: 8.8 coord: 238..270 e-value: 3300.0 score: 3.5 coord: 328..349 e-value: 790.0 score: 8.0
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 139..418 e-value: 1.3E-46 score: 159.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 25..423 e-value: 1.2E-124 score: 418.5
None	No IPR available	PANTHER	PTHR31339	PECTIN LYASE-RELATED	coord: 7..449
None	No IPR available	PANTHER	PTHR31339:SF20	OS07G0245200 PROTEIN	coord: 7..449
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 38..434

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh18G006640.1	CmaCh18G006640.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity