CmaCh18G006450 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh18G006450
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionNicalin
LocationCma_Chr18: 5854670 .. 5864668 (-)
RNA-Seq ExpressionCmaCh18G006450
SyntenyCmaCh18G006450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCCTCCTAAAGCACGCGAGCGACAGGTGCTCGAATCCTTCTACCCTGTTCTCGCTCTCGTCTTCATTCTTGTCGCCTGTGTCGAGTTCTGTGACGCCGCCACTGTTGTGGATGTCTACCGTCTCATTCAGTACGATATCTCTGGTGTTCCATTTGGATCTCGCGCCGCCACACTTAATCACCATGCTGGCTCTCTTCATTTTCCCTCTGGTGCTGATCTCTCTCGCACTGTTGTCATCAGTCCTCTTTGTGAACTCAATATCACCTTTGTCAAGGGTACGATTCTTCTATTTTTTTTTTTTTTTTTTTTTTTAATTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTAACACTTGTCATTCTCGCACTTTCTTAGCTGGTGGAGCATGAATACGACGCTTGTATATCGTTAAAATGTGGAGATTTCAGTAGAGCAGTTCTGAACTGTTTTTAACTGTTGGCGGATTGAATTACTCTCATAGATTCAGTTTTTGATTGTTGCTACTTCATTTGGGTTCTTTTTGAAATTCCACATAAAGTTTCATTGGGGGAAGTTTCCTTGACGACCGGGCTTTAATTACGGAACAAGTTAAAGAAGTTTGCTCTTTGTGTATTTCTTTTACAATATGCCAAATTTGGAAGGTTCGATTCTTCTTTCTATTATCAAACAATTCTGTGCTTGTAGACTTGCAGAACAGTGTTAATCTAATTTATCATGTACTTGAAGAATGAAGGGTATGATTGTAGACTTTGATTGATTTTAAACACTATTCATTTTATCAACGATAAAGAAAGGTTCTTCAAATAGCTTTACCTCCTTCAATAAAGTCAGGAAATAGAGGTTAGGGGAAAGAGATTTTCATCCAGCAGCTTTGCCTTGTGAAAGAAAGAGTGAAAGAAGAATTTGAGGATTTCTTCTTATTCACCGAGCAAAAGCAGCTAATTCATTTACTCGACTGTAAAGGATTGAATGAATTTACATTTCTTACTTTGTAACATTTTACATTTCAGAAATCCTGCCTGTTTTTGTCAGTGAGAAGATTGTGTGATCTTACATATGTGTACTATGAGAAGTGTCGGTATACTATTCCCGCTCTTCCTTCTCCTCTGTTGGTAACTGGGATATCTTTTTGGTCTTGTGCAGAATGTATATCTCAAAAAAAGCGTCTGGGAGGTCTGCTGTTTTTGCTTCCAAAGATTCTTGGCTTGGATGGCCTGAAAAACGAGGGTACTAGATGTCCGAATAATGGAGAGGGGATGATCAAGGATTTATTGGCTCAACTTGAACGGTTCCTCATACATGAAAATATACCTGTGAGTGAAATGAAAATAACTTCTGGAATTTCTTCACTTCACCAGATATTTCAATAGTTATTCAGTGCTTCTATGTTTGGTTCTTCTATATATGATTGAAACATTTGAACATTTAACTTCTTTTTATATTAAGTGCTGCTAGAAAGAAATGTGAACTTTATTTTCTTTCTACATTAAATGCACCCGATCTTTTTTCAGTCTGAATTATGACCACATTTTTGTATTACATTTCTTCAATATATATTTTCAATTTCTATTTTTTCTGAGAGTATCTATGTCGTTCGTTTCAGTATCCTGTATATTTTGCTTCAGAAGGTGATGATATTGATGCTGTTTTGGCTGATGTCAAGAGGAATGATGCCACTGGTCAGCTTGCAACTGCAACTACTGGCGGGTTAGTCGAATCTATTGTAACTTTTGGGTGCTTTAGTGGAGATTTTATATGGATAAATGAATTTATATCACAACTGATATAGGAAAGAATAGTTGTTTTTGGAATCACAAAATAATTTTTATCATTTATGATAATTTTGTATTATATTATATTTTTTTGATTCTGACTTCAATATGATTATTATGTTTTGTTTTTTGCAACATAAGCCTTTTATTGCAACAGGAAACAACACTAAGTATTGAATGTTGAGTTATATTATGTTCTCATCTTTTATATTCACCACTATCTCTAATTTTTTCCTCAGTTCTCTGTCTGATCCCCCTTTTGCATAAAATATTGACGATATTTATCTTGTAACTTCCTTTATCCTTAAGTTCTTGCCTAAGGAGTTAAATATCATTGTTGGCTAAAGTGTCGAGATTTCAATGAAGATTTTTCGAAAAATCTTAGAAATCTGTAAAGTTTCTACACTTCTCAAGTAACTTATAAAATCATGATTATTATTAATGTTGTTTTCTTTTTGTGAAATTCCTAATATTTCTGGACACTTTCGATGTGTTATTGACATTTGCACAGTGTTAAAGCAAACATCTATCAACCTGTAATATTCATATCAAACCATTCTGTTTTTCGTTTAAAAAGTGCTTGCAGTTTAAACTCTCCTTGTGCCTTTTGCAAGAGCTTTTATTTAATAGTTCATTCTCTCTTCAATCTTATTTCTCATAAAAAATAACTTGGGAGCTTGAGGATACTATATGATTGCTAATCTTCTCTGTGTTCATGTCTGTCAGATACAAGCTTGTTGTTTCAGCACCAGAACCAAGGAAACTTGTATCTTCCACAATTACAAACATTCAGGTACCTGTTTTGGTTTCCTTAAAATGTTAACAACTAGAATTGTTACATATAATTATTTATTTGAGTACAAACATTCAGGTATCTGCATTATGCTTTTAAGCAACACGTTGGAGACCAATTGATTGCCTTGAAATCATACATAATAGCGGATTGGAAATCCTTTCTAAAGAAAAAGTGTTTGGCTTGTTGGGTTTCTAGTTTTCTTTAAGATTGTACCCAAGCAATTACTGCATGGTCGAGTTTGTTGCCCTCTTTCACCTTTGTACATGCATGTGGTTTTTTACTACAGAAATAAATTATGACTTGCGCTCTGCCTTGAATGGATTGCTATGTTAATGAGACTGCTAATTTACATTATCCTAGCCCGCCCTTTTTTCTGATGGCTTCTCTGCTGCAGTACTTTTCATAATTTCCATTTTCTTAATGTCCATTACAAATTTCCACCTTACCCACCTTATGAAAGGGCACTTAAGAAATTTCCTCCTTTATATTGTGAGGTTTTATTTACCGGATTTTTTTTTATTGCTATGCTGGCTTAACCAAGGGCCATAGACCGAAGGAAAAATTTGACTTTCGGAGTATTGGGTATCCATACCACATGAGAAAGGTGTCCTAGGGATAATATCTGTGTAATGCTTTGTTTTGGACCCCCTAACCCCTTTCAGTTTAATTGGCCGCTCTGTCAAATCTCTCTGTTAAACTAGATTGCACTTACTGGAGACTTCCTTTGCTAGAGATATTTGGAAAGACCATTGCCCAAAGATCAATTATTTCCTACGGGAAATTTCTCACATAGCCATTAATAGCCAAGCTTTCATCCAAAGGAGATTGGCATTTGCTTAGAGATTTGGGAATGATACACAACGTTTATGGTTGGTTTTGGGTGTTTCCTAGGGATGTTAAGGAGCTTCTTACCACTGTTTTGTTGTGTCACCCCTTCAAGAAAGCCAAGGCCCTGCTGTGGATGCACATATCTAGGGCCTTCTTTTGTGTCCTTTGGAAGAAAAGAAACCAGAGTCTTGTAGGATTAAAAACACCCTTTTGACCGTTCTTCAACCTCATGGATTTTACATCAGTTTTGTAAATTTCATGCATCAACGAAATTGTTTCTTATAAAAAATAAAAAATAAAAAGTGATTTGAAATTTTCACATGGCATTTATGTTAATACTCTCTTTTGACCTCTAAAAATATTGGAGGGGTTATGGATACTGGTCAAGTGAGAGCTAGCGTGTTAAAAATACTACAGCCTAAATTCTGTATTTTCCTTATAACTTACCGTTTCCATTGTTGGTGCAATTGTCTAGTTAGTGTTCTGTTATTGAGTTGCTGTGATTAGCTTTATTCTCCCCTAATATTTTGTGTCTAATTCTTTTCAATAGTGTTACATCGTTTTTTCCCCTAAAAATTTGTGTCAGGGTTGGCTGCCAGGACTAAAATCTGACGGAGATGCTAGTCAACTCCCAACAATTGCTATTATAGCATCATATGATACATTTGGCGCTGCTCCTGTATGTTATTTGTTTAACGCACCAACAGTATCGACCATATTTTATCTATATTATATCTAGATGATGTGGATCCTCTCTCTGGTTGTAGGACTTATCTGTGGGAAGTGATAGCAACGGAAGTGGAATTGTTGCACTTCTTGAAATTGCAAGGTTATTCTCTCTTCTTTATTCCAACCCTAAGACAAGAGGAAGGTATAACCTACTTTTTGGGCTCACTTCCGGCGGACCTTACAACTATAATGGGACTCACAAGGTTCTCATCCTTTCTATGTGTATACAATTGTCATAATTCTTACTTGTTTTTATCATTACAATGTATTCCATTTACTTTATTTTCTTCATTTTAATTATTATGTATTTATTTATTTATTTTATTATTTACTTTATCACTGTTTCCAGTTCTCATTCTTTCTATGTATATACAATTGTCATATTTCTTGTTTTTATCATTACAAAGTATTCCATTTAGTTAATTTTCTTCATTTTAATTATTTTATTATTTACTTTATCATTGTTTCCATTTGTTGTCCACCAATGTAAAATATCGCTTTTCCAGTGGCTTCAAAGCTTGGATCACCGTCTCCATGAGAGTATTGACTATGCTATTTGCTTAAATAGTATTGGCTCATGGGATGACAAATTGTGGCTGCATGTCTCTAAACCTCCAGAAAATGCCTACATCAAAAAAATCTTTGAAGTAAGCAAATGTGCTTTTAAAATTTTAATGTCTTATGACTGTTATTTCATTGTAACGTGTTGATTGTGGAGGACTGGACTCTGTAGGATTTCTCAAATGTTGCCGAGGATTTGGGCTTTAAAGTTGATCTGAAGCACAAGAAGATTAATATTTCAAACCCTCGAGTGAGTAAATACACCTGAAATATTTCTTTTTTGACAGAAAATTAATTTTTGGCTGAAGATAATATTATGAAACTGTATAATTCGTATTTAAAAATTTGATATGAATAAAGGCATCTCAATCATGCAATATGAACTTCGAGGAAGATGTAGAAAATGTTGTTCAAATTACCACAAGCTAAACTCTAGTCACATTAAGTGAAGTCAAGTTTCATTAAGTTGAACTCATGTTGCAATTAATGATACATAAAAAAAATTAAGTTTCATTTGTGCGAAAATGGATTCAAGCTACTGCAAGTGAGACTTTATTCATTTTTGAATGGTTACAAATTCTTGGGTGGCTGATTGAAATCTACTATTTTCAAATTTGTAGTTTTTCATGAGTGTATTTTCAGTTTTATGATTTTTTATTTACTTTTTAGTTACATTTACATAATGACTATAATATGAAGGGTACAACTCTTGAAATGCCTCATGAAAGGTCAAAGAGGACTTGAAAAATGTGGTAAAAAAAAACATTTGTACTTTCTTTTAGTCTAGAGCTAAATTCTTTACAATTCTATTTAGTTTAGGTTGCATGCATAAATCATTCAATCTTGACTTGATCAATCTTGCTTGTGGAGTGATTCGAATCTTAAATAAAATTTCTTATCTTGAGATATTTGATCAATAAGGTAACTTGGATCTTCCCTTGAGTAATTCCTTAATTTTTTTTGGGGTTTTTGGATATTTTAGATCTAAAAGTCTTTTTTCCAATAAGTTTGTTAGGTCAAATTCTCGGGAGTTTCATATAATTTGGGATGAAATCATCTTGATTTGAAGTTTTTTAGCATCTGTGATCTAGGGGTTTGCATATCTTTAAGGTTCTTAGATTCAATTAGCTAAGGTTTTCATATCCCAATTCATATAAAAATTAAAACATTTATTGAAATGTTACATACATACATACATACATACATACATACATATATATATATATACATACATACATACATATATTAAATAGAAAGCGGAAGTAGAAGACCGTTTGAAAGGTTTTGTTCTCTCCCGAGAACAAGCCTAATTCCAACCTACCTGTTACATACATCCATTTACACCTACGATCTTTCTATAATAACAAGCCGAGGGTGTCCATGCCCTTGCTTATGTCATCCCTCGTGCTTCCCCACCCTTCGTTCTACGTGCGCATACAAATCTAATGGATGGTTTAGTAAGCCTGTCATTCTGCACATTGATGATACAAAAGAACTATAAAGATTTTTGTGACTGGACAACATACATCTCCGTTATTACATTTTTTCCCACATGATGTGTGCTATTTCCTTAGCATGGTATACTATTGTTACTTTTATTTTTATGATTATCGTAGTCCGCTCCATAATTCCTTTGTATGCTGTAGGTAGCCTGGGAGCACGAACAGTTTTCAAGATTGAGAGTAACTGCTGCTACCCTTTCTGGACTTTCTACTGCTCCTGAGCTTTTGGAAAGGACTGGAGGTTTAGTTGATAACAGGTTCTGTTTTTAAGAAATTAATCAATTTCTCGGTTCTGTTCCACTTCATGTTTGCATGTGTGCGTTATATATTTGTGTATGCGTGTTCTTTGTATTCTGACAAGATCATAACTTCCAACAGATCATTTTTGAACGAGAGTGCAGTTGCCAAGAGTATCAAGTTAGTTGCGGAGAGCCTTGCAGTAAGATTTGTCATGTCTTGCTTAGCCATAATCTTTAAGCTATTTCAACTTCTTCCTGTTTCCAACTGCTAGTGTTTTTTTTTTTTTTGGCAATCCAGAACTCATTTCTAGATTTGAATGTTTAAATATGTCACTCAACTTCTACTGTTATTTAAAAGTGGGGAGAGTGCACGAATACATTACATACATTATTGATTTTCAAATTGCAGATGACTCAGAATGTCTGCTGTATTATTTTATATTGTTTTTAGGATTCTCTAATGCATAATTCCTTTTATTTATGCAGAGGCATATTTACAGATATGATGGAAAGAACATACAAGTATTTGCAGATGATAGTAGTTTGGCAGTCCATCCAACATATATTCGATCATGGTTGGATCTTTTGTCACGATCACCTCGAGTTGCTCCATTTCTGTCGAAGGACGATCCCTTCATATTGGCATTGAAAAAGGTTTTATATCATAACTAAAATTGTTCCAGTTGATGCCATCTACTTTCATTTAGCTTCCTATACTGTATTCACCCAAAGACTGATTGATTGACAATCTGATAAAGAAAATAGAAGACAATGTTCTGAAACATGTAGATGTTCAACTTGGCAAGTTTCCTGTCATAAGATAACCCCTTCCTAGGCTGTTACCATTCTTGTCCTTTCATTCAGGTTAACCCGAAAATATTGCTCTAAAACATACATAAAATGCTTGGGGTTTTTAATTTTCAGCTGCATACAACATTCTGAATTTTGATAAAATTGCTGCCATCTTTTTGAACCATAATTTAAATTTGTTACATCATAAAATCCTGACATTTTTTTTTTAATATGGTGGAACTATTACTGTTGCTTCTACTCTATCCAAATGTGTCTTGCACGTTTGTGTGCTTCAAAAGTTTGCAAATCATTTGATCTAACACAATATCTGCGTGGTCAGTTAGTTTGACTGGGCTGACTCATAAGTCATACGAATACCTTAGGTTACTAACTCAAGTTACCTAAGCCATCGTCCAATGATATTGTTCTACTATTTTCAGGAACTGGAGGTCCATACCAGTGATGTGAGCTTGCAACATGAGCTATTCGATGGGATGTTCACCTTTTATGATTCAACTGCAGCTAAACTTCACATATATCAGGTACTTTGCAGATGAATGCTGAACTTGATTCTGTACTTGTATTTTCATGTTACAGATCCAGTTTTGTTATAATTTTCCAGCTTCTCCAATCACACATGCTCATTCTGAATCTACAGTTCTATTTTAGTTTGTACATAGTTATTTCCTATGTTCGAGTCAGCTGATTCTGGATCTGTGGTTATACATGCATGTGAAATGGGATGAGGGTTGCTTTCTCTATTTAAGTCCTTCGGTGTTCTTTCTTGACTTCAATCTAAGCAAGCTTTATATTTGTTGTTTCAGGTTGCTAGCGTGACGTTCGACTTGCTTTTGCTTTTGGCCTTGGGATCATATTTAGTTTTTCTCTTCTGTTTTCTTGTGATCACAACCAGGGTATGTTCTATCCTTTTCTTAATCAATTTTAGAAATCTCTCTCATGATATAAAATTATACCCGAACTTCTTTACATCTGCGTAAAACACTTATTTTAAATGCATTCTCAAGCACACTTTTAGAGTAGGTGTGACATGACTTTTTAAAATAATGTTTTTTTCAGTGAATCTTTTTTATAAGCACTCTACAGAAACACTCTCTAGAATCGATTTCAAGTGCTTTTAAGAATTTCTAAATTATTTTTATTATGTAATCAAACGTTATACAAAATCTTAAAAAGTAATTGTAATTGGTTAAAAAATGGTAGAAAAGCTCACAAAATGTTTTTGTTTCATAGGGTCTTGATGATCTGATCGGTCTATTTAGACGCCCTCCCTCCCGAAAAGTGAAAACAGCTTGATGAGTATTGTAGATTTTGATGCTTGGATTTACATCATTGCTGCCAAATTTTTGCCACCTAGATTAGACAGGCCATACACTATAGGGTCCAATGTGACAAGGTGTCAAACCAATCATCACAGCTAGATGGAACTGAAGGTTTCATTTCATGTGGTATTATTCCTCTCAGGATTTTCTCATCCTTTTCTCTTTTCCTGACATCCTTTAGTTATATCATCTGTGCCATAATAACGTTATTGTGTGCTCTCCGTATTTAATTTCGTACTGTTTCACCCTCAATTGTGGTTTCCACTTTTGACTAAGATTTCTTTTCTTGAAACTTGTTGGGTCTTATTTGGAGCACCGTCGATGTTAGTGGCAAAAATACATTAGATTCTGTTCAACAGTTAAAAGTCATGAGATAAGAGTTTTCTTTCCCTCCATTTTTTCAGAGAATTTGTATGGGATTTTGACTGTTGGTATTAGAATTAACTTTCAGGTTAGTCATTAGTTTGTAATTGTGTTAACTATTATCCAGTTCTATCAAATCTCTGTAGTATTTCTGTTTAAAAGCATGGACTTACTAAGAGATAATATACAAGATTTTATCTCACAAATTTCGAATTCGATTTCAAAATAGTAACGTTTCTGCTGCTTCACAAACTTTCTTTTGCACTTTCTTTTCATTTTACAGATGCTACAGTCTAGAATACATACTTTGTACATTATTATGATTGGTTAAATCTTGTTCCTGTTATATGGGCTTCGTACCGACAATGTATCGTGGAGCCGTTAGATGATTATAGCTCTTTATATCAAGAACAAATTAGGGTTCCTCAATGGAACTGTTTCCAAACACTGGTGAATTATTATTACCTGCTTGGACTTGAAAATGGCATCTTGAATGCCTGGATTTTAAGTTCGCTCAGAACTGATTGTTGCTTGTATCACTTACTAGAATTCTGCATATGGCATTCGGGTTGATTTTAAGGGAAGATTCCAGCGACAAATCGGGTTTGTGGTAATCATGAACCAGATAATCATGATTGTCTAAAACAATATGAATTCAACGTAGACAACTATATGAGACACTGTTCCATGGCCTCCACATTATAACCACCATCTAGCTTTTACTTTACTTGACTCC

mRNA sequence

ATGGCTCCTCCTAAAGCACGCGAGCGACAGGTGCTCGAATCCTTCTACCCTGTTCTCGCTCTCGTCTTCATTCTTGTCGCCTGTGTCGAGTTCTGTGACGCCGCCACTGTTGTGGATGTCTACCGTCTCATTCAGTACGATATCTCTGGTGTTCCATTTGGATCTCGCGCCGCCACACTTAATCACCATGCTGGCTCTCTTCATTTTCCCTCTGGTGCTGATCTCTCTCGCACTGTTGTCATCAGTCCTCTTTGTGAACTCAATATCACCTTTGTCAAGGAATGTATATCTCAAAAAAAGCGTCTGGGAGGTCTGCTGTTTTTGCTTCCAAAGATTCTTGGCTTGGATGGCCTGAAAAACGAGGGTACTAGATGTCCGAATAATGGAGAGGGGATGATCAAGGATTTATTGGCTCAACTTGAACGGTTCCTCATACATGAAAATATACCTTATCCTGTATATTTTGCTTCAGAAGGTGATGATATTGATGCTGTTTTGGCTGATGTCAAGAGGAATGATGCCACTGGTCAGCTTGCAACTGCAACTACTGGCGGATACAAGCTTGTTGTTTCAGCACCAGAACCAAGGAAACTTGTATCTTCCACAATTACAAACATTCAGGGTTGGCTGCCAGGACTAAAATCTGACGGAGATGCTAGTCAACTCCCAACAATTGCTATTATAGCATCATATGATACATTTGGCGCTGCTCCTGACTTATCTGTGGGAAGTGATAGCAACGGAAGTGGAATTGTTGCACTTCTTGAAATTGCAAGGTTATTCTCTCTTCTTTATTCCAACCCTAAGACAAGAGGAAGGTATAACCTACTTTTTGGGCTCACTTCCGGCGGACCTTACAACTATAATGGGACTCACAAGTGGCTTCAAAGCTTGGATCACCGTCTCCATGAGAGTATTGACTATGCTATTTGCTTAAATAGTATTGGCTCATGGGATGACAAATTGTGGCTGCATGTCTCTAAACCTCCAGAAAATGCCTACATCAAAAAAATCTTTGAAGATTTCTCAAATGTTGCCGAGGATTTGGGCTTTAAAGTTGATCTGAAGCACAAGAAGATTAATATTTCAAACCCTCGAGTAGCCTGGGAGCACGAACAGTTTTCAAGATTGAGAGTAACTGCTGCTACCCTTTCTGGACTTTCTACTGCTCCTGAGCTTTTGGAAAGGACTGGAGGTTTAGTTGATAACAGATCATTTTTGAACGAGAGTGCAGTTGCCAAGAGTATCAAGTTAGTTGCGGAGAGCCTTGCAAGGCATATTTACAGATATGATGGAAAGAACATACAAGTATTTGCAGATGATAGTAGTTTGGCAGTCCATCCAACATATATTCGATCATGGTTGGATCTTTTGTCACGATCACCTCGAGTTGCTCCATTTCTGTCGAAGGACGATCCCTTCATATTGGCATTGAAAAAGGAACTGGAGGTCCATACCAGTGATGTGAGCTTGCAACATGAGCTATTCGATGGGATGTTCACCTTTTATGATTCAACTGCAGCTAAACTTCACATATATCAGGTTGCTAGCGTGACGTTCGACTTGCTTTTGCTTTTGGCCTTGGGATCATATTTAGTTTTTCTCTTCTGTTTTCTTGTGATCACAACCAGGGGTCTTGATGATCTGATCGGTCTATTTAGACGCCCTCCCTCCCGAAAAGTGAAAACAGCTTGATGAGTATTGTAGATTTTGATGCTTGGATTTACATCATTGCTGCCAAATTTTTGCCACCTAGATTAGACAGGCCATACACTATAGGGTCCAATGTGACAAGGTGTCAAACCAATCATCACAGCTAGATGGAACTGAAGGTTTCATTTCATGTGAATTCTGCATATGGCATTCGGGTTGATTTTAAGGGAAGATTCCAGCGACAAATCGGGTTTGTGGTAATCATGAACCAGATAATCATGATTGTCTAAAACAATATGAATTCAACGTAGACAACTATATGAGACACTGTTCCATGGCCTCCACATTATAACCACCATCTAGCTTTTACTTTACTTGACTCC

Coding sequence (CDS)

ATGGCTCCTCCTAAAGCACGCGAGCGACAGGTGCTCGAATCCTTCTACCCTGTTCTCGCTCTCGTCTTCATTCTTGTCGCCTGTGTCGAGTTCTGTGACGCCGCCACTGTTGTGGATGTCTACCGTCTCATTCAGTACGATATCTCTGGTGTTCCATTTGGATCTCGCGCCGCCACACTTAATCACCATGCTGGCTCTCTTCATTTTCCCTCTGGTGCTGATCTCTCTCGCACTGTTGTCATCAGTCCTCTTTGTGAACTCAATATCACCTTTGTCAAGGAATGTATATCTCAAAAAAAGCGTCTGGGAGGTCTGCTGTTTTTGCTTCCAAAGATTCTTGGCTTGGATGGCCTGAAAAACGAGGGTACTAGATGTCCGAATAATGGAGAGGGGATGATCAAGGATTTATTGGCTCAACTTGAACGGTTCCTCATACATGAAAATATACCTTATCCTGTATATTTTGCTTCAGAAGGTGATGATATTGATGCTGTTTTGGCTGATGTCAAGAGGAATGATGCCACTGGTCAGCTTGCAACTGCAACTACTGGCGGATACAAGCTTGTTGTTTCAGCACCAGAACCAAGGAAACTTGTATCTTCCACAATTACAAACATTCAGGGTTGGCTGCCAGGACTAAAATCTGACGGAGATGCTAGTCAACTCCCAACAATTGCTATTATAGCATCATATGATACATTTGGCGCTGCTCCTGACTTATCTGTGGGAAGTGATAGCAACGGAAGTGGAATTGTTGCACTTCTTGAAATTGCAAGGTTATTCTCTCTTCTTTATTCCAACCCTAAGACAAGAGGAAGGTATAACCTACTTTTTGGGCTCACTTCCGGCGGACCTTACAACTATAATGGGACTCACAAGTGGCTTCAAAGCTTGGATCACCGTCTCCATGAGAGTATTGACTATGCTATTTGCTTAAATAGTATTGGCTCATGGGATGACAAATTGTGGCTGCATGTCTCTAAACCTCCAGAAAATGCCTACATCAAAAAAATCTTTGAAGATTTCTCAAATGTTGCCGAGGATTTGGGCTTTAAAGTTGATCTGAAGCACAAGAAGATTAATATTTCAAACCCTCGAGTAGCCTGGGAGCACGAACAGTTTTCAAGATTGAGAGTAACTGCTGCTACCCTTTCTGGACTTTCTACTGCTCCTGAGCTTTTGGAAAGGACTGGAGGTTTAGTTGATAACAGATCATTTTTGAACGAGAGTGCAGTTGCCAAGAGTATCAAGTTAGTTGCGGAGAGCCTTGCAAGGCATATTTACAGATATGATGGAAAGAACATACAAGTATTTGCAGATGATAGTAGTTTGGCAGTCCATCCAACATATATTCGATCATGGTTGGATCTTTTGTCACGATCACCTCGAGTTGCTCCATTTCTGTCGAAGGACGATCCCTTCATATTGGCATTGAAAAAGGAACTGGAGGTCCATACCAGTGATGTGAGCTTGCAACATGAGCTATTCGATGGGATGTTCACCTTTTATGATTCAACTGCAGCTAAACTTCACATATATCAGGTTGCTAGCGTGACGTTCGACTTGCTTTTGCTTTTGGCCTTGGGATCATATTTAGTTTTTCTCTTCTGTTTTCTTGTGATCACAACCAGGGGTCTTGATGATCTGATCGGTCTATTTAGACGCCCTCCCTCCCGAAAAGTGAAAACAGCTTGA

Protein sequence

MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATLNHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKNEGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLATATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDLSVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDHRLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKINISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVAESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKKELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLVITTRGLDDLIGLFRRPPSRKVKTA
Homology
BLAST of CmaCh18G006450 vs. ExPASy Swiss-Prot
Match: Q6NZ07 (Nicalin-1 OS=Danio rerio OX=7955 GN=ncl1 PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.4e-67
Identity = 194/595 (32.61%), Postives = 308/595 (51.76%), Query Frame = 0

Query: 11  VLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATLNHHAGSLHFP 70
           +L+  +P+  ++F+++ C    +AA    VYR+ QYD+ G  +GSR A LN  A ++   
Sbjct: 12  MLKVSFPLSLVLFLVLVCPLRAEAAHEFSVYRMQQYDLQGQTYGSRNAILNTEARTV--- 71

Query: 71  SGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKNEGTRCPNNGE 130
               LSR  V+  L + +    ++ + Q    G ++ +L                P+N  
Sbjct: 72  EAEVLSRRCVMMRLADFSYEKYQKALRQS--AGAVVIIL----------------PHNMS 131

Query: 131 GMIKDLLAQ---LERFLIHENIPYPVYFASEGDDIDAVLADVK--------RNDATGQLA 190
            + +D++ Q   LE  L+      PVYFA E +++ ++    +         + A   L 
Sbjct: 132 TLPQDIVQQFMELEPELLATETIVPVYFALEDEELLSIYTQTQISSSSQGSSSAAEVLLH 191

Query: 191 TATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPD 250
           TAT  G+++V S  + + +    IT+++G L G  S G+   LPTI ++A YD+FG AP 
Sbjct: 192 TATANGFQMVTSGAQSKAVSDWAITSLEGRLTG--SGGE--DLPTIVLVAHYDSFGVAPW 251

Query: 251 LSVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQ-SL 310
           LS G+DSNGSG+  LLE+ARLFS LYS  +T   YNLLF L+ GG +NY GT +WL+ +L
Sbjct: 252 LSYGADSNGSGVAILLELARLFSRLYSYKRTHAGYNLLFFLSGGGKFNYQGTKRWLEDNL 311

Query: 311 DHR----LHESIDYAICLNSIGSWDDKLWLHVSKPPEN-----AYIKKIFEDFSNVAEDL 370
           DH     L +++ + +CL+++G+  D L LHVSKPP+        +K++    ++   DL
Sbjct: 312 DHTDASLLQDNVAFVLCLDTLGN-SDNLHLHVSKPPKEGSPQYTLLKELETVVAHQHPDL 371

Query: 371 GFKVDLKHKKINISNPRVAWEHEQFSRLRVTAATLSGL---------------STAPELL 430
            F   + HKKIN+++  +AWEHE+F   R+ A TLS L               S +P L 
Sbjct: 372 KFA--MVHKKINLADDTLAWEHERFGIRRLPAFTLSHLESHRSPARHSIMDMRSVSPSL- 431

Query: 431 ERTGGLVDNRSFLNESAVAKSIKLVAESLARHIYRYDGK----NIQVFADDSSLAVHPTY 490
               G       ++   ++++ K++AE+LAR IY    K    ++++F +   + V    
Sbjct: 432 -EGAGEATTGPHVDLGKLSRNTKVIAETLARVIYNLTEKGVTGDLEIFTE--QMQVQEDQ 491

Query: 491 IRSWLDLLSRSPRVAPFLSKDDPFILALKKELEVHTSDVS---LQHELFDGMFTFYDSTA 550
           + S +D L+  PR A  L KD   I  L+ +L  +  DV    ++ +  D  F FYD   
Sbjct: 492 LASLVDWLTAQPRAAQLLDKDSSIINTLEHQLSRYLKDVKRHLVRADKRDPEFVFYDQLK 551

Query: 551 AKLHIYQVASVTFDLLLLLALGSYLVFLFCFLVITTRGLDDLIGLFRRPPSRKVK 563
             ++ Y+V    FDLLL + + SYL  L  +L I   GL  L G  RR  + +VK
Sbjct: 552 QTMNAYRVKPAIFDLLLAVCIASYLGVL--YLAIQNFGL--LYGFLRRVTAPRVK 570

BLAST of CmaCh18G006450 vs. ExPASy Swiss-Prot
Match: Q8VCM8 (Nicalin OS=Mus musculus OX=10090 GN=Ncln PE=1 SV=2)

HSP 1 Score: 256.9 bits (655), Expect = 5.3e-67
Identity = 184/548 (33.58%), Postives = 291/548 (53.10%), Query Frame = 0

Query: 15  FYPVLALVFILVA-CVEFCDAATVVDVYRLIQYDISGVPFGSRAATLNHHAGSLHFPSGA 74
           F   L  V +LVA  +   DAA    VYR+ QYD+ G P+G+R A LN  A ++     A
Sbjct: 22  FIVFLPAVLLLVAPPLPAADAAHEFTVYRMQQYDLQGQPYGTRNAVLNTEARTV----DA 81

Query: 75  D-LSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKNEGTRCPNNGEGM 134
           D LSR  V+  L + +    ++ + Q    G ++ +LP+ +                + +
Sbjct: 82  DVLSRRCVLMRLLDFSYEHYQKALRQS--AGAVVIILPRAMAA------------VPQDV 141

Query: 135 IKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATG--------QLATATTG 194
           ++  + ++E  ++      PVYFA E + + ++    +   A+          L TAT  
Sbjct: 142 VRQFM-EIEPEMLAMETVVPVYFAVEDEALLSIYEQTQAASASQGSASAAEVLLHTATAN 201

Query: 195 GYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDLSVGS 254
           G+++V S  + + +    IT+++G L GL  +     LPTI I+A YD FG AP LS+G+
Sbjct: 202 GFQMVTSGAQSQAVSDWLITSVEGRLTGLGGE----DLPTIVIVAHYDAFGVAPWLSLGA 261

Query: 255 DSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQ-SLDHR-- 314
           DSNGSGI  LLE+ARLFS LY+  +T   YNLLF  + GG +NY GT +WL+ SLDH   
Sbjct: 262 DSNGSGISVLLELARLFSRLYTYKRTHAAYNLLFFASGGGKFNYQGTKRWLEDSLDHTDS 321

Query: 315 --LHESIDYAICLNSIGSWDDKLWLHVSKPP-----ENAYIKKIFEDFSNVAEDLGFKVD 374
             L +++ + +CL+++G     L LHVSKPP     ++A+++++    ++   D+ F   
Sbjct: 322 SLLQDNVAFVLCLDTVGR-GSHLRLHVSKPPREGTLQHAFLRELETVAAHQFPDVSF--S 381

Query: 375 LKHKKINISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTG---GLVDNRSFLNESA 434
           + HKKIN+++  +AWEHE+F+  R+ A TLS L +      R G    ++D RS ++   
Sbjct: 382 MVHKKINLADDVLAWEHERFAIRRLPAFTLSHLES-----HRAGPRSSIMDVRSRVDSKT 441

Query: 435 VAKSIKLVAESLARHIYRYDGK----NIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPF 494
           + ++ +++AE+L R IY    K    ++ VF +   + V    I S +D L+  PR A  
Sbjct: 442 LTRNTRIIAEALTRVIYNLTEKGTPPDMPVFTE--QMQVQEEQIDSVMDWLTNQPRAAQL 501

Query: 495 LSKDDPFILALKKELEVHTSDVSLQH---ELFDGMFTFYDSTAAKLHIYQVASVTFDLLL 533
           L KD  F+  L+  L  +  DV   H   +  D  F FYD     ++ Y+V    FDLLL
Sbjct: 502 LDKDGTFLSTLEHFLSRYLKDVRQHHVKADKRDPEFVFYDQLKQVMNAYRVKPAIFDLLL 536

BLAST of CmaCh18G006450 vs. ExPASy Swiss-Prot
Match: Q5XIA1 (Nicalin OS=Rattus norvegicus OX=10116 GN=Ncln PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 6.9e-67
Identity = 186/548 (33.94%), Postives = 287/548 (52.37%), Query Frame = 0

Query: 15  FYPVLALVFILVA-CVEFCDAATVVDVYRLIQYDISGVPFGSRAATLNHHAGSLHFPSGA 74
           F   L  V +LVA  +   DAA    VYR+ QYD+ G P+G+R A LN  A ++     A
Sbjct: 22  FIVFLPAVLLLVAPPLPAADAAHEFTVYRMQQYDLQGQPYGTRNAVLNTEARTV----DA 81

Query: 75  D-LSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKNEGTRCPNNGEGM 134
           D LSR  V+  L + +    ++ + Q    G ++ +LP+ +                + +
Sbjct: 82  DVLSRRCVLMRLLDFSYEHYQKALRQS--AGAVVIILPRAMAA------------VPQDV 141

Query: 135 IKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATG--------QLATATTG 194
           ++  + ++E  ++      PVYFA E + + ++    +   A+          L TAT  
Sbjct: 142 VRQFM-EIEPEMLAMETVVPVYFAVEDEALLSIYEQTQAASASQGSASAAEVLLHTATAN 201

Query: 195 GYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDLSVGS 254
           G+++V S  + + +    IT+++G L GL  +     LPTI I+A YD FG AP LS+G+
Sbjct: 202 GFQMVTSGAQSQAVSDWLITSVEGRLTGLGGE----DLPTIVIVAHYDAFGVAPWLSLGA 261

Query: 255 DSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQ-SLDHR-- 314
           DSNGSGI  LLE+ARLFS LY+  +T   YNLLF  + GG +NY GT +WL+ SLDH   
Sbjct: 262 DSNGSGISVLLELARLFSRLYTYKRTHAAYNLLFFASGGGKFNYQGTKRWLEDSLDHTDS 321

Query: 315 --LHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIF-EDFSNVA----EDLGFKVD 374
             L +++ + +CL+++G     L LHVSKPP    ++ +F  +   VA     D+ F   
Sbjct: 322 SLLQDNVAFVLCLDTVGR-GSHLRLHVSKPPREGTLQHVFLRELEMVAAHQFPDVSF--S 381

Query: 375 LKHKKINISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTG---GLVDNRSFLNESA 434
           + HKKIN+++  +AWEHE+F+  R+ A TLS L        R G    ++D RS ++   
Sbjct: 382 MVHKKINLADDVLAWEHERFAIRRLPAFTLSHLEN-----HRAGPRSSIMDVRSRVDSKT 441

Query: 435 VAKSIKLVAESLARHIYRYDGK----NIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPF 494
           + ++ +++AE+L R IY    K    ++ VF +   + V    I S +D L+  PR A  
Sbjct: 442 LTRNTRIIAEALTRVIYNLTEKGTPPDMPVFTE--QMQVQQEQIDSVMDWLTNQPRAAQL 501

Query: 495 LSKDDPFILALKKELEVHTSDVSLQH---ELFDGMFTFYDSTAAKLHIYQVASVTFDLLL 533
           L KD  F+  L+  L  +  DV   H   +  D  F FYD     ++ Y+V    FDLLL
Sbjct: 502 LDKDGTFLSTLEHFLSRYLKDVRQHHVKADKRDPEFVFYDQLKQVMNAYRVKPAIFDLLL 536

BLAST of CmaCh18G006450 vs. ExPASy Swiss-Prot
Match: Q969V3 (Nicalin OS=Homo sapiens OX=9606 GN=NCLN PE=1 SV=2)

HSP 1 Score: 249.6 bits (636), Expect = 8.4e-65
Identity = 174/545 (31.93%), Postives = 283/545 (51.93%), Query Frame = 0

Query: 15  FYPVLALVFILVA-CVEFCDAATVVDVYRLIQYDISGVPFGSRAATLNHHAGSLHFPSGA 74
           F   L  V +LVA  +   DAA    VYR+ QYD+ G P+G+R A LN  A ++   +  
Sbjct: 22  FIVFLPAVLLLVAPPLPAADAAHEFTVYRMQQYDLQGQPYGTRNAVLNTEARTM---AAE 81

Query: 75  DLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKNEGTRCPNNGEGMI 134
            LSR  V+  L + +    ++ + Q    G ++ +LP+ +                + ++
Sbjct: 82  VLSRRCVLMRLLDFSYEQYQKALRQS--AGAVVIILPRAMAA------------VPQDVV 141

Query: 135 KDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQ-----------LATAT 194
           +  + ++E  ++      PVYFA E    +A+L+  K+  A              L TAT
Sbjct: 142 RQFM-EIEPEMLAMETAVPVYFAVED---EALLSIYKQTQAASASQGSASAAEVLLRTAT 201

Query: 195 TGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDLSV 254
             G+++V S  + + +    I +++G L GL  +     LPTI I+A YD FG AP LS+
Sbjct: 202 ANGFQMVTSGVQSKAVSDWLIASVEGRLTGLGGE----DLPTIVIVAHYDAFGVAPWLSL 261

Query: 255 GSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQ-SLDHR 314
           G+DSNGSG+  LLE+ARLFS LY+  +T   YNLLF  + GG +NY GT +WL+ +LDH 
Sbjct: 262 GADSNGSGVSVLLELARLFSRLYTYKRTHAAYNLLFFASGGGKFNYQGTKRWLEDNLDHT 321

Query: 315 ----LHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIF-EDFSNVA--EDLGFKVD 374
               L +++ + +CL+++G     L LHVSKPP    ++  F  +   VA  +    +  
Sbjct: 322 DSSLLQDNVAFVLCLDTVGR-GSSLHLHVSKPPREGTLQHAFLRELETVAAHQFPEVRFS 381

Query: 375 LKHKKINISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAK 434
           + HK+IN++   +AWEHE+F+  R+ A TLS L +  +   +   ++D RS ++   + +
Sbjct: 382 MVHKRINLAEDVLAWEHERFAIRRLPAFTLSHLESHRD--GQRSSIMDVRSRVDSKTLTR 441

Query: 435 SIKLVAESLARHIYRYDGK----NIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSK 494
           + +++AE+L R IY    K    ++ VF +   + +    + S +D L+  PR A  + K
Sbjct: 442 NTRIIAEALTRVIYNLTEKGTPPDMPVFTE--QMQIQQEQLDSVMDWLTNQPRAAQLVDK 501

Query: 495 DDPFILALKKELEVHTSDVSLQH---ELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLA 533
           D  F+  L+  L  +  DV   H   +  D  F FYD     ++ Y+V    FDLLL + 
Sbjct: 502 DSTFLSTLEHHLSRYLKDVKQHHVKADKRDPEFVFYDQLKQVMNAYRVKPAVFDLLLAVG 536

BLAST of CmaCh18G006450 vs. ExPASy Swiss-Prot
Match: Q5ZJH2 (Nicalin OS=Gallus gallus OX=9031 GN=NCLN PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 8.4e-57
Identity = 168/548 (30.66%), Postives = 276/548 (50.36%), Query Frame = 0

Query: 14  SFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATLNHHAGSLHFPSGA 73
           SF   +  V +L+      +AA    VYR+ QY++ G P+G+R+A LN  A ++     A
Sbjct: 21  SFLLFVPAVLLLLGPPPAAEAAHESTVYRMQQYELGGQPYGTRSAVLNTEARTVE----A 80

Query: 74  D-LSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKNEGTRCPNNGEGM 133
           D LSR  V+  L + +    ++ + Q    G ++ +LP+ +             +  + +
Sbjct: 81  DVLSRRCVMMRLVDFSYEQYQKALRQS--AGAVVIILPRSIS------------SVPQDV 140

Query: 134 IKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATG--------QLATATTG 193
           +K  +      L  E I  PVYFA E D++ ++    +   A+          L TAT  
Sbjct: 141 VKQFMEIEPEMLAMETI-VPVYFAVEDDELLSIYEQTRAASASQGSASAAEVLLHTATAN 200

Query: 194 GYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDLSVGS 253
           G+++V S  + + +    I +++G L GL  +     LPT+ I+A YD+FG AP LS G+
Sbjct: 201 GFQMVTSGAQSKAIHDWLIPSVEGRLTGLGGE----DLPTVVIVAHYDSFGVAPWLSHGA 260

Query: 254 DSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQ-SLDHR-- 313
           DSNGSG+  LLE+ARLFS LY+  +T   YNLLF  + GG +NY GT +WL+ +LDH   
Sbjct: 261 DSNGSGVSVLLELARLFSRLYTYRRTHAGYNLLFFASGGGKFNYQGTKRWLEDNLDHTDS 320

Query: 314 --LHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGF---KVDLK 373
             L +++ + +CL+++G   + L LHVSKPP+   ++  F     +     F   K  + 
Sbjct: 321 SLLQDNVAFVLCLDTLGR-GNSLHLHVSKPPKEGTLQHAFLRELEMVVASQFPEVKFSMV 380

Query: 374 HKKINISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSI 433
           HKKIN++   +AWEHE+F+  R+ A T+S L +  + L  +  ++D RS ++  A+ +  
Sbjct: 381 HKKINLAEDILAWEHERFAIRRLPAFTISHLESHRDSLRNS--IMDRRSQVDTKALTQEY 440

Query: 434 KLVAESLARHIYRYDGKNIQVFADDS---SLAVHPT------YIRSWLDLLSRSPRVAPF 493
           +        H   +D  ++Q   + S   S  +H +        R    L  +S + A  
Sbjct: 441 Q-------DHCGGFDEGHLQPNREGSTCRSADLHGSDADPGGAARISDGLAEQSAQAAQL 500

Query: 494 LSKDDPFILALKKELEVHTSDVSLQH---ELFDGMFTFYDSTAAKLHIYQVASVTFDLLL 533
           + KD  F+  L+  +  +  DV   H   +  D  F FYD     ++ Y+V    FDLLL
Sbjct: 501 IDKDSTFLSTLEYYMGRYLKDVKQHHVKADKRDPEFVFYDQLKQVMNAYRVKPAIFDLLL 535

BLAST of CmaCh18G006450 vs. ExPASy TrEMBL
Match: A0A6J1HVN9 (nicalin-1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466578 PE=4 SV=1)

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 564/564 (100.00%), Postives = 564/564 (100.00%), Query Frame = 0

Query: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
           MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60

Query: 61  NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120
           NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN
Sbjct: 61  NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120

Query: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
           EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT
Sbjct: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180

Query: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL 240
           ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL
Sbjct: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL 240

Query: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
           SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300

Query: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
           RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360

Query: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
           NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420

Query: 421 ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK 480
           ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK 480

Query: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV 540
           ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV
Sbjct: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV 540

Query: 541 ITTRGLDDLIGLFRRPPSRKVKTA 565
           ITTRGLDDLIGLFRRPPSRKVKTA
Sbjct: 541 ITTRGLDDLIGLFRRPPSRKVKTA 564

BLAST of CmaCh18G006450 vs. ExPASy TrEMBL
Match: A0A6J1G0U3 (Nicalin OS=Cucurbita moschata OX=3662 GN=LOC111449666 PE=3 SV=1)

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 556/564 (98.58%), Postives = 563/564 (99.82%), Query Frame = 0

Query: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
           MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60

Query: 61  NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120
           NHHAGSLHFPSGADLSRTV+I+PLCELNITFVKECISQKKRLGGLLFL+PKILGLDGLKN
Sbjct: 61  NHHAGSLHFPSGADLSRTVIITPLCELNITFVKECISQKKRLGGLLFLVPKILGLDGLKN 120

Query: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
           EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT
Sbjct: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180

Query: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL 240
           ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAI+ASYDTFGAAPDL
Sbjct: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPDL 240

Query: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
           SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300

Query: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
           R+HESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RIHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360

Query: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
           NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420

Query: 421 ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK 480
           ESLARHIYRYDGKNIQVFADDSSLAV+PTYIRSWLDLLSR+PRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYDGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 480

Query: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV 540
           ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLV LFCFLV
Sbjct: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVLLFCFLV 540

Query: 541 ITTRGLDDLIGLFRRPPSRKVKTA 565
           ITTRGLDDLIGLFRRPPSRKVKTA
Sbjct: 541 ITTRGLDDLIGLFRRPPSRKVKTA 564

BLAST of CmaCh18G006450 vs. ExPASy TrEMBL
Match: A0A6J1CME5 (Nicalin OS=Momordica charantia OX=3673 GN=LOC111012612 PE=3 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 1.9e-301
Identity = 522/564 (92.55%), Postives = 538/564 (95.39%), Query Frame = 0

Query: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
           MAP KARER+VLESFYPV+ALVFILVACVE CDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1   MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 60

Query: 61  NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120
           NHHAGSLHFP GADLSRTVVI PLCELNITFVKECISQKK LGGLLFLLPKI G D  KN
Sbjct: 61  NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKKXLGGLLFLLPKIFGSDDTKN 120

Query: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
           +GT+CPNNGEG +K+LL +LER L+HENIPYPVYFASEG+DI AVLADVKRNDATGQLAT
Sbjct: 121 DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT 180

Query: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL 240
           ATTGGYKLVVSA EPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAI+ASYDTFGAAP+L
Sbjct: 181 ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL 240

Query: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
           SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQS DH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH 300

Query: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
           RL ESIDYAICLNSIGSWDDKLWLHVSKPPEN YIK+IFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 360

Query: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
           NISN RVAWEHEQFSRLRVTAATLS LS APELLERTGGLVDNR FLNESA+AKSIKLVA
Sbjct: 361 NISNLRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA 420

Query: 421 ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK 480
           ESLARHIYRY+GKNIQVFADDSSLAV+PTYIRSWLDLLSR PRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRMPRVAPFLSKDDPFILALKK 480

Query: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV 540
           ELEVHT DVS+QHE FDGMFTFYDSTAAKLHIYQVASVTFDLLLLL LGSYLV LFCFLV
Sbjct: 481 ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV 540

Query: 541 ITTRGLDDLIGLFRRPPSRKVKTA 565
           ITTRGLDDLIGLFRRPPSRKVKTA
Sbjct: 541 ITTRGLDDLIGLFRRPPSRKVKTA 564

BLAST of CmaCh18G006450 vs. ExPASy TrEMBL
Match: A0A6J1IC52 (nicalin-1-like OS=Cucurbita maxima OX=3661 GN=LOC111471642 PE=4 SV=1)

HSP 1 Score: 1006.5 bits (2601), Expect = 4.3e-290
Identity = 502/567 (88.54%), Postives = 529/567 (93.30%), Query Frame = 0

Query: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
           MAP K RE QVLESFYP+LALVF+LVAC E CDAA VVDVYRLI YDISGVPFGSRAA+L
Sbjct: 1   MAPRKPREPQVLESFYPLLALVFLLVACTELCDAAAVVDVYRLIHYDISGVPFGSRAASL 60

Query: 61  NHHAGSLHFP---SGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDG 120
           NHHA SLHFP   + ADLSRTV I PLCELN TFVKEC+SQ+KRLGGLL LLPKILG DG
Sbjct: 61  NHHAASLHFPPAAAAADLSRTVFIIPLCELNFTFVKECLSQRKRLGGLLILLPKILGSDG 120

Query: 121 LKNEGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQ 180
            KN+  +CP NG+GMIKDLL +LER LIH  +PYPVYFASEG+DI+AVLADVK NDATGQ
Sbjct: 121 PKNDDFKCPQNGDGMIKDLLVELERLLIHATLPYPVYFASEGEDINAVLADVKSNDATGQ 180

Query: 181 LATATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAA 240
           LATATTGGYKLVVSA EPRKLVSSTITNIQGWLPGLK DGDASQLPTIAI+ASYDTFGA+
Sbjct: 181 LATATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKFDGDASQLPTIAIVASYDTFGAS 240

Query: 241 PDLSVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQS 300
           P+LSVGSDSNGSGIVALLEIARLFSLLYS+PKTRGRYNLLFGLTSGGPYNYNGTHKWLQS
Sbjct: 241 PELSVGSDSNGSGIVALLEIARLFSLLYSSPKTRGRYNLLFGLTSGGPYNYNGTHKWLQS 300

Query: 301 LDHRLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKH 360
            DHR+ ESIDYAICLNSIGSWDDKLWLHVSKPPENAYIK+IFEDFSNVAEDLGFKVDLKH
Sbjct: 301 FDHRIRESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKQIFEDFSNVAEDLGFKVDLKH 360

Query: 361 KKINISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIK 420
           KKINISNPRVAWEHEQFSRLRVTAATLSG+S APELLERTGGL DNR FLNESA+AKSIK
Sbjct: 361 KKINISNPRVAWEHEQFSRLRVTAATLSGISAAPELLERTGGLADNRLFLNESAIAKSIK 420

Query: 421 LVAESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILA 480
           LVAESLARHIYRY+GKNIQVFADDSSLAV+PTYIRSWLDLLSR+PRVAPFLSKDDPFI A
Sbjct: 421 LVAESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFISA 480

Query: 481 LKKELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFC 540
           LKKELEVHT DVSLQHE FDG+FTFYDSTAAKLH+YQVASVTFDL+LLL LGSYLV LFC
Sbjct: 481 LKKELEVHTHDVSLQHEPFDGVFTFYDSTAAKLHVYQVASVTFDLVLLLVLGSYLVLLFC 540

Query: 541 FLVITTRGLDDLIGLFRRPPSRKVKTA 565
           FLVITTRGLDDLIGLFRRPPSRKVKTA
Sbjct: 541 FLVITTRGLDDLIGLFRRPPSRKVKTA 567

BLAST of CmaCh18G006450 vs. ExPASy TrEMBL
Match: A0A1S3CJR5 (nicalin-1 OS=Cucumis melo OX=3656 GN=LOC103501746 PE=4 SV=1)

HSP 1 Score: 1006.1 bits (2600), Expect = 5.7e-290
Identity = 503/564 (89.18%), Postives = 531/564 (94.15%), Query Frame = 0

Query: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
           MAP K RE QVL+SFYPVLALVFILVACVE CDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1   MAPRKPREPQVLDSFYPVLALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 60

Query: 61  NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120
           NHHA SLHFPSGADLSRTV+I PLCEL +TF++ECISQKKRLGGLL LLP+ILG + LKN
Sbjct: 61  NHHASSLHFPSGADLSRTVLIIPLCELKMTFLQECISQKKRLGGLLVLLPRILGSESLKN 120

Query: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
           +  +C  NGEG+IKDLL +LER LIH  IPYPVYFAS+G+DIDAVLADVK NDATGQLAT
Sbjct: 121 DDIKC-TNGEGVIKDLLVELERLLIHSTIPYPVYFASDGEDIDAVLADVKNNDATGQLAT 180

Query: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL 240
           ATTGGYKLVVSA EP+KL+SSTITNIQGWLPGLKSDGDASQLPTIAI+ASYDTFGAAP+L
Sbjct: 181 ATTGGYKLVVSAAEPKKLISSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL 240

Query: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
           SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQS DH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH 300

Query: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
           RL E IDYAICLNSIGSWDDKLWLHVSKPPENAYIK+IFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RLRERIDYAICLNSIGSWDDKLWLHVSKPPENAYIKQIFEDFSNVAEDLGFKVDLKHKKI 360

Query: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
           NISNPRVAWEHEQFSRLRVTAATLS LS APELLERTGGL DNR FL+ES +AKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLGDNRLFLDESKIAKSIKLVA 420

Query: 421 ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK 480
           ESLARHIYRY+GKNIQVFADDSSLAV+PTYIRSW+DLLSR+PRVAPFLSKDDPFI ALKK
Sbjct: 421 ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWVDLLSRTPRVAPFLSKDDPFISALKK 480

Query: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV 540
           ELEVHT DVSLQHE+F+G+FTFY STAAKLH+YQVASVTFDLLLLL LGSYLV LFCFLV
Sbjct: 481 ELEVHTHDVSLQHEVFEGIFTFYGSTAAKLHVYQVASVTFDLLLLLVLGSYLVLLFCFLV 540

Query: 541 ITTRGLDDLIGLFRRPPSRKVKTA 565
           ITTRGLDDLIGLFRRPPSRKVKTA
Sbjct: 541 ITTRGLDDLIGLFRRPPSRKVKTA 563

BLAST of CmaCh18G006450 vs. NCBI nr
Match: XP_022967064.1 (nicalin-1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 564/564 (100.00%), Postives = 564/564 (100.00%), Query Frame = 0

Query: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
           MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60

Query: 61  NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120
           NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN
Sbjct: 61  NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120

Query: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
           EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT
Sbjct: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180

Query: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL 240
           ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL
Sbjct: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL 240

Query: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
           SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300

Query: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
           RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360

Query: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
           NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420

Query: 421 ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK 480
           ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK 480

Query: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV 540
           ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV
Sbjct: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV 540

Query: 541 ITTRGLDDLIGLFRRPPSRKVKTA 565
           ITTRGLDDLIGLFRRPPSRKVKTA
Sbjct: 541 ITTRGLDDLIGLFRRPPSRKVKTA 564

BLAST of CmaCh18G006450 vs. NCBI nr
Match: KAG7012715.1 (Nicalin [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1113.6 bits (2879), Expect = 0.0e+00
Identity = 558/564 (98.94%), Postives = 563/564 (99.82%), Query Frame = 0

Query: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
           MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60

Query: 61  NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120
           NHHAGSLHFPSGADLSRTVVI+PLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN
Sbjct: 61  NHHAGSLHFPSGADLSRTVVITPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120

Query: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
           EGTRCPNNGEGMIK+LLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT
Sbjct: 121 EGTRCPNNGEGMIKELLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180

Query: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL 240
           ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAI+ASYDTFGAAPDL
Sbjct: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPDL 240

Query: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
           SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300

Query: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
           RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360

Query: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
           NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420

Query: 421 ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK 480
           ESLARHIYRYDGKNIQVFADDSSLAV+PTYIRSWLDLLSR+PRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYDGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 480

Query: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV 540
           ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLV LFCFLV
Sbjct: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVLLFCFLV 540

Query: 541 ITTRGLDDLIGLFRRPPSRKVKTA 565
           ITTRGLDDLIGLFRRPPSRKVKTA
Sbjct: 541 ITTRGLDDLIGLFRRPPSRKVKTA 564

BLAST of CmaCh18G006450 vs. NCBI nr
Match: XP_022945431.1 (nicalin-1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 556/564 (98.58%), Postives = 563/564 (99.82%), Query Frame = 0

Query: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
           MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60

Query: 61  NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120
           NHHAGSLHFPSGADLSRTV+I+PLCELNITFVKECISQKKRLGGLLFL+PKILGLDGLKN
Sbjct: 61  NHHAGSLHFPSGADLSRTVIITPLCELNITFVKECISQKKRLGGLLFLVPKILGLDGLKN 120

Query: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
           EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT
Sbjct: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180

Query: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL 240
           ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAI+ASYDTFGAAPDL
Sbjct: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPDL 240

Query: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
           SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300

Query: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
           R+HESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RIHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360

Query: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
           NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420

Query: 421 ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK 480
           ESLARHIYRYDGKNIQVFADDSSLAV+PTYIRSWLDLLSR+PRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYDGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 480

Query: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV 540
           ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLV LFCFLV
Sbjct: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVLLFCFLV 540

Query: 541 ITTRGLDDLIGLFRRPPSRKVKTA 565
           ITTRGLDDLIGLFRRPPSRKVKTA
Sbjct: 541 ITTRGLDDLIGLFRRPPSRKVKTA 564

BLAST of CmaCh18G006450 vs. NCBI nr
Match: KAG6573634.1 (Nicalin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 558/564 (98.94%), Postives = 562/564 (99.65%), Query Frame = 0

Query: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
           MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60

Query: 61  NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120
           NHHAGSLHFPSGADLSRTVVI+PLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN
Sbjct: 61  NHHAGSLHFPSGADLSRTVVITPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120

Query: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
           EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT
Sbjct: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180

Query: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL 240
           ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAI+ASYDTFGAAPDL
Sbjct: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPDL 240

Query: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
           SVGSDSNGSG VALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH
Sbjct: 241 SVGSDSNGSGSVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300

Query: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
           RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360

Query: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
           NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420

Query: 421 ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK 480
           ESLARHIYRYDGKNIQVFADDSSLAV+PTYIRSWLDLLSR+PRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYDGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 480

Query: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV 540
           ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLV LFCFLV
Sbjct: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVLLFCFLV 540

Query: 541 ITTRGLDDLIGLFRRPPSRKVKTA 565
           ITTRGLDDLIGLFRRPPSRKVKTA
Sbjct: 541 ITTRGLDDLIGLFRRPPSRKVKTA 564

BLAST of CmaCh18G006450 vs. NCBI nr
Match: XP_023542146.1 (nicalin-1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 557/564 (98.76%), Postives = 563/564 (99.82%), Query Frame = 0

Query: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
           MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1   MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60

Query: 61  NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120
           NHHAGSLHFPSGADLSRTVVI+PLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN
Sbjct: 61  NHHAGSLHFPSGADLSRTVVITPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120

Query: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
           EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT
Sbjct: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180

Query: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL 240
           ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAI+ASYDTFGAAPDL
Sbjct: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPDL 240

Query: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
           SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300

Query: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
           RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360

Query: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
           NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420

Query: 421 ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK 480
           ESLARHIYRYDGKNIQVFADDSSLAV+PTYIRSWLDLLS++PRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYDGKNIQVFADDSSLAVNPTYIRSWLDLLSQTPRVAPFLSKDDPFILALKK 480

Query: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV 540
           ELEVHTSDVSLQHELFDGMFTFYDSTAAKL+IYQVASVTFDLLLLLALGSYLV LFCFLV
Sbjct: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLNIYQVASVTFDLLLLLALGSYLVLLFCFLV 540

Query: 541 ITTRGLDDLIGLFRRPPSRKVKTA 565
           ITTRGLDDLIGLFRRPPSRKVKTA
Sbjct: 541 ITTRGLDDLIGLFRRPPSRKVKTA 564

BLAST of CmaCh18G006450 vs. TAIR 10
Match: AT3G44330.1 (INVOLVED IN: protein processing; LOCATED IN: mitochondrion, endoplasmic reticulum, plasma membrane, vacuole; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Nicalin (InterPro:IPR016574), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Nicastrin (InterPro:IPR008710); Has 245 Blast hits to 243 proteins in 99 species: Archae - 6; Bacteria - 10; Metazoa - 139; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 44 (source: NCBI BLink). )

HSP 1 Score: 817.4 bits (2110), Expect = 7.2e-237
Identity = 410/559 (73.35%), Postives = 472/559 (84.44%), Query Frame = 0

Query: 5   KARER-QVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATLNHH 64
           K R R  V ES YP+LAL+ ILVACVE CDAATVVDVYRLIQYDISGVPFGSR ++LNHH
Sbjct: 8   KQRHRVMVFESMYPILALMLILVACVELCDAATVVDVYRLIQYDISGVPFGSRFSSLNHH 67

Query: 65  AGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKNEGT 124
           A SL F  GADLSR+V+I PL EL+I FV++ ISQK+ LGGLL LLP+      +     
Sbjct: 68  AASLSFQRGADLSRSVLILPLRELDIAFVQDYISQKQSLGGLLILLPQTFRPGNVGGGSL 127

Query: 125 RCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLATATT 184
              N+G    + LL QLE+ L+H NIP+PVYFA E ++ DA+LADVK+NDA GQ ATATT
Sbjct: 128 SSENDG---FRSLLGQLEKLLVHGNIPFPVYFAFENEETDAMLADVKKNDALGQQATATT 187

Query: 185 GGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDLSVG 244
           GGYKLV+S  EPRK+ S TITNIQGWLPGL+++GD+SQLPTIA++ASYDTFGAAP LSVG
Sbjct: 188 GGYKLVISVSEPRKIASPTITNIQGWLPGLRAEGDSSQLPTIAVVASYDTFGAAPALSVG 247

Query: 245 SDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDHRLH 304
           SDSNGSG+VALLE+ARLFS+LYSNPKTRG+YNLLF LTSGGPYNY GT KWL+SLD R+ 
Sbjct: 248 SDSNGSGVVALLEVARLFSVLYSNPKTRGKYNLLFALTSGGPYNYEGTQKWLKSLDQRMR 307

Query: 305 ESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKINIS 364
           ESIDYAICLNS+GSWD +L +HVSKPP+NAYIK+IFE FSNVAEDLGF+V LKHKKINIS
Sbjct: 308 ESIDYAICLNSVGSWDSELLIHVSKPPDNAYIKQIFEGFSNVAEDLGFQVALKHKKINIS 367

Query: 365 NPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVAESL 424
           N RVAWEHEQFSRLRVTAATLS LST PELLE  G L D R  +NE A+ K +KLVAESL
Sbjct: 368 NSRVAWEHEQFSRLRVTAATLSELSTPPELLENAGSLSDTRQLVNEDAIIKGVKLVAESL 427

Query: 425 ARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKKELE 484
           A+HIY + GK+I++FADDSSLAV+P Y+RSWLDLLS++PRVAPFLSK++P I+ALKKELE
Sbjct: 428 AKHIYGHQGKDIKIFADDSSLAVNPFYVRSWLDLLSQTPRVAPFLSKNEPLIMALKKELE 487

Query: 485 VHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLVITT 544
            +T++VS+QHE  DG FTFYDST A L+IYQVASVTFDLLLLL LGSYL+ LF FLVITT
Sbjct: 488 DYTAEVSVQHESLDGSFTFYDSTKASLNIYQVASVTFDLLLLLVLGSYLIVLFSFLVITT 547

Query: 545 RGLDDLIGLFRRPPSRKVK 563
           RGLDDLI LFRRPPSRKVK
Sbjct: 548 RGLDDLISLFRRPPSRKVK 563

BLAST of CmaCh18G006450 vs. TAIR 10
Match: AT3G52640.2 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 5.0e-04
Identity = 44/169 (26.04%), Postives = 72/169 (42.60%), Query Frame = 0

Query: 144 LIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLATATTGGYKLVVSAPEPRK------ 203
           ++  N  +PVY  SE   I AV   + +        T+    + +V+   +         
Sbjct: 162 IMWRNYNFPVYLLSE-SGISAVHEILSKKKMKHGTYTSDVAEFNMVMETTKAGTHNSEAC 221

Query: 204 LVSSTITNIQGW-----LPGLKSDGDASQLPTIAIIASYDTFGAAPDLSVGSDSNGSGIV 263
           L   T   + G+     LP +      ++ P +  +AS DT     D S G+DS  SG+V
Sbjct: 222 LQEGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLTVASMDTASFFRDKSFGADSPISGLV 281

Query: 264 ALLEIARLFSLL--YSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLD 300
           ALL      S +   SN K +    L+F + +G  + Y G+ ++L  LD
Sbjct: 282 ALLGAVDALSRVDGISNLKKQ----LVFLVLTGETWGYLGSRRFLHELD 325

BLAST of CmaCh18G006450 vs. TAIR 10
Match: AT3G52640.1 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 5.0e-04
Identity = 44/169 (26.04%), Postives = 72/169 (42.60%), Query Frame = 0

Query: 144 LIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLATATTGGYKLVVSAPEPRK------ 203
           ++  N  +PVY  SE   I AV   + +        T+    + +V+   +         
Sbjct: 162 IMWRNYNFPVYLLSE-SGISAVHEILSKKKMKHGTYTSDVAEFNMVMETTKAGTHNSEAC 221

Query: 204 LVSSTITNIQGW-----LPGLKSDGDASQLPTIAIIASYDTFGAAPDLSVGSDSNGSGIV 263
           L   T   + G+     LP +      ++ P +  +AS DT     D S G+DS  SG+V
Sbjct: 222 LQEGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLTVASMDTASFFRDKSFGADSPISGLV 281

Query: 264 ALLEIARLFSLL--YSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLD 300
           ALL      S +   SN K +    L+F + +G  + Y G+ ++L  LD
Sbjct: 282 ALLGAVDALSRVDGISNLKKQ----LVFLVLTGETWGYLGSRRFLHELD 325

BLAST of CmaCh18G006450 vs. TAIR 10
Match: AT3G52640.3 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 5.0e-04
Identity = 44/169 (26.04%), Postives = 72/169 (42.60%), Query Frame = 0

Query: 144 LIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLATATTGGYKLVVSAPEPRK------ 203
           ++  N  +PVY  SE   I AV   + +        T+    + +V+   +         
Sbjct: 162 IMWRNYNFPVYLLSE-SGISAVHEILSKKKMKHGTYTSDVAEFNMVMETTKAGTHNSEAC 221

Query: 204 LVSSTITNIQGW-----LPGLKSDGDASQLPTIAIIASYDTFGAAPDLSVGSDSNGSGIV 263
           L   T   + G+     LP +      ++ P +  +AS DT     D S G+DS  SG+V
Sbjct: 222 LQEGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLTVASMDTASFFRDKSFGADSPISGLV 281

Query: 264 ALLEIARLFSLL--YSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLD 300
           ALL      S +   SN K +    L+F + +G  + Y G+ ++L  LD
Sbjct: 282 ALLGAVDALSRVDGISNLKKQ----LVFLVLTGETWGYLGSRRFLHELD 325

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NZ071.4e-6732.61Nicalin-1 OS=Danio rerio OX=7955 GN=ncl1 PE=2 SV=1[more]
Q8VCM85.3e-6733.58Nicalin OS=Mus musculus OX=10090 GN=Ncln PE=1 SV=2[more]
Q5XIA16.9e-6733.94Nicalin OS=Rattus norvegicus OX=10116 GN=Ncln PE=2 SV=1[more]
Q969V38.4e-6531.93Nicalin OS=Homo sapiens OX=9606 GN=NCLN PE=1 SV=2[more]
Q5ZJH28.4e-5730.66Nicalin OS=Gallus gallus OX=9031 GN=NCLN PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HVN90.0e+00100.00nicalin-1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466578 PE=4 SV=1[more]
A0A6J1G0U30.0e+0098.58Nicalin OS=Cucurbita moschata OX=3662 GN=LOC111449666 PE=3 SV=1[more]
A0A6J1CME51.9e-30192.55Nicalin OS=Momordica charantia OX=3673 GN=LOC111012612 PE=3 SV=1[more]
A0A6J1IC524.3e-29088.54nicalin-1-like OS=Cucurbita maxima OX=3661 GN=LOC111471642 PE=4 SV=1[more]
A0A1S3CJR55.7e-29089.18nicalin-1 OS=Cucumis melo OX=3656 GN=LOC103501746 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022967064.10.0e+00100.00nicalin-1-like isoform X1 [Cucurbita maxima][more]
KAG7012715.10.0e+0098.94Nicalin [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022945431.10.0e+0098.58nicalin-1-like isoform X1 [Cucurbita moschata][more]
KAG6573634.10.0e+0098.94Nicalin, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023542146.10.0e+0098.76nicalin-1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G44330.17.2e-23773.35INVOLVED IN: protein processing; LOCATED IN: mitochondrion, endoplasmic reticulu... [more]
AT3G52640.25.0e-0426.04Zn-dependent exopeptidases superfamily protein [more]
AT3G52640.15.0e-0426.04Zn-dependent exopeptidases superfamily protein [more]
AT3G52640.35.0e-0426.04Zn-dependent exopeptidases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF05450Nicastrincoord: 225..361
e-value: 8.5E-9
score: 35.2
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 148..422
e-value: 1.2E-21
score: 79.6
NoneNo IPR availablePANTHERPTHR31826:SF7NICALINcoord: 10..563
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..28
score: 6.0
NoneNo IPR availableCDDcd03882M28_nicalin_likecoord: 137..428
e-value: 7.48359E-114
score: 338.573
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 195..424
IPR016574NicalinPANTHERPTHR31826NICALINcoord: 10..563
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 245..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G006450.1CmaCh18G006450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009966 regulation of signal transduction
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane