CmaCh18G005450 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh18G005450
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRING/U-box superfamily protein
LocationCma_Chr18: 3504430 .. 3507845 (-)
RNA-Seq ExpressionCmaCh18G005450
SyntenyCmaCh18G005450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGACAATGAATGCCATAAACAGCAAAAGTAACCCATTTTTTCTTTAATCTTAAGGCATCAGATTAGCAAATAGTAACTCAAGATTCTTCCCTTTTCTCTCTCTGAAAAACCCAATTGATGGATCCGCCTCTTTTCTAGAGAGAGAATTTGGGGCGCTGCTTTGGTGCTTATTCTTGCAGTTTGTCAGATTAGGGTTTTTTTTCTGTTTTCTTTTTTTGTTTTTAATATCATTTCTTCCACAGACATATCTTCCAGGCTCCAGAATTCCCTTCACATGCGGTGAAGTTCTTATGGTATGGAACATTCATTCATTTCTTTGATTTCTTTTTTCTTACGTTTCTTCATTCTCCGACCCCCAATCCCCGAGTTTTTGTGAATTTTTGTTTGGTTCTTATAAATGGGGTTTGGTGGATTACGTAGTTGATTAATGGGTCGATCTTGATTTGCACAGAAAATACAGGAACTGCTTGTTTTGTTTACGTTTTCTCCGATTCGGACATGTTCCTCTTTTTCGTATTCGATTTTTGGAATTTCGTGTCCTCTAATGTGGAAATGGAATAGATTTGATGGAAATTTCCGAGTGATTTATATTGTTTTCTGTGATTTTAGGAGGGAGTGTTGCTTCAGTTGGCAAATTAGGGAAATCTTGCTCCCGATTGTTTATTTGGAGTTCTTCTTAAACAGCGGATGAGTAATTAAGAACTGCGAAGAACGTTCTTAAATTCATTTGCTGGTGGATCTTCGCTTATTTGCTGCCATCTGGGATTTGTGGATCGTTTGAAATTTTTTAACAAAATGGAGCATCGTGAGATGTTCGGTCCAGCTGAGGAAGATGATGTTGAAGATTTTCGAAGTTGTTGTGAAGATCATGAGGATGTGGTGGAAGAAGGGTCTGAGGAGGAGGAGGATCTTGATGAGTTTTCGGTGAAAATGTTCTTTAAGGGCATTTCGATAGCCGAGATTGGTGACCCGTCTTCTGGTCTTTCTGGTATTGGAGTGTTCATGGAAAGATCAGGGAAAGTCCCTTTTATTCAGGTCCAGAAAAAGCTTGATTTCTATGTTGATGAGCTAGTTGCTGATTATTTAGCTTTGATGGATGGATTGGTTGAGGCCATACAGAATGGCGTCCGCTCTATTCGAGCTTTTACCGACTCCAATACGTTATATAATCAGGTTACTACTTCCCCTATCTGCGTAACTTTGCTTGAAAGATTGCATAAACTCTTAAATTTATGTGCATTTTCTGAAGATATGTGAATGTTTGACAGATAACAAATGAAGAGGAGCCTGAAATTCCGCTTCTTGTAGCGCTGAAAGAAAGGATTCTAGAGCATGCTAGCACTTTTGAAGCGTTTTCTCTGAACCTTTCGTCGCATTCAGACCTCGAGCAGCCATTTCATCTCGCGAAAGTGGCTATTGGGGTTCTTTCTTGTCCACAAAAGGGAGATAAATCAATTGAGAATTGTTCGATTTGTTGTGAGAACAAACCGACCGCTATGATGGTTGCGTTGAAGTGTTGTCATAAGTTCTGCTCTCATTGTATGAAGAGTTATGTTGATGGAAAATTAGAGACATCTCAAGTTCCTATCAGATGCCCTCAGCTGAGATGCAAATATTACATCACAAGCAATGAGTTTAAATCTTTTCTTCCCTTGACTTTATATGAATCGTTGGAGAATACCTTAGCCGAAGCGAATATTCACTCAGATAAAATTTACTGCCCTTATCCGAATTGCTCTGTTCTGTTAGACCCGAGCGAATGTTTGTCAGCTCGGGCAAGTTCATCAAGTCAATCGGATAACACTTGTATTGAATGTCCAGTTTGTCAGAGGTTTATTTGTGTTGAATGTTGTGTTCCTTGGCATTCATCAATGAGCTGTGAAGAGTTTCAGGACATCCCATTAGAGGAAAGAGACATTGCTGATATTACCTTACATCGTCTTGCACGAAACAAAAGGTGGCGACGTTGTCAGCAGTGTCGTAGGATGATTGAATTGGCTCAAGGCTGCTACCATATGACATGCTGGTAATGCTACTTCCTCGAAATCTCTCGTTATTTATGTTAACGTGTCGGTTTATGAGATAATTGTGGAAGCACCTGAATCATCGTGAATATATATCATTAGTTGAGTGGAATGTTAGACTTTTTGAAGGATCAGCTTGTAGGACTTGATTAGAAAGTTAGAGGCACTTTATATACTTATTTGCACGTCGTAAACGTGATCTGGTATTTGTACGTATGGTCTATACATCTATGGCCAGGCACGTGGTTTGATGTTTGCACGTCGTAAACATGATCTGATATTTGCACGTATGGTCTATGCATCTATGGCCAGGCACATGTGGTTTGATATTTGCACGTCGTAAACGTGATTTGATATTTGCACGTTGTAAACGTGATTTGATATTTGCTCGTCATAAACGTGATTTGATATTTGCACGTATGGTCTATAATCTATGGTCAGGCTCACGTGGTTTGATATTTGCACGTCGTAAACGTGATCAGCAAATTGCAATGCTTTGATTTTACTAAACTAGGTGCAGGGTTTTCTTTATGTATATGAATTCAATGCTTATACTGATATGTTGCAGGTGCGGGCATGAATTCTGCTATTCTTGTGGTGCTGAGTACCAAGATGGGCAACAGACCTGCCAATGCGCCTTTTGGGACGACGACGATGACGACGACAACTCCGAGGACTTGGTTACCCACTCCATCCAAGAATCTGAGCAGTGGGCATGGGAAACTTTTAATTCCCTCCCGATGATAACGGACGCATACTCAGAGCAAGAGAGATCACAGTTAGCACTAATCCAACGATTCCTGGCTGGGGGATTCAGTCTGAGTGACCACCAACCGTATCAATCGCCACCACGATGTACAGACTCTTATGTCGACACGATGAAGGACCTACACCAACTTCCATGGCTTGAGAGGTTCGTTTCCGTGATAAGTGATAACTACTATGAAGATTACGTACAATGATATATCGATTCTTAGACGAAAAATAAAAGGGGGAGGTAAAAGAGCCTCTCACTCTAGTTTGCCTCCCTCGATACCTCATTTACACCGCCGCACAGCTTGGCGTTTCGATCGCTAGTTACGCTCTGTTTGTCTGTTGAATCATATACTGGAAATTATGAAGTGACCCTCAGAGGTCCTCATTGCATTAGATTAGATTAGAATGAAGGAATCTTATGCAAGAATGTCAGCTTTGCTTGTTTGATTCTTTCCTGTTTCAAACAAAAGAAAAAGACAAGAAATAGTCAAGAATTTAATCAAAATGAAAATTATGTTCGAGTTGAGAGTATATGTTTCGATGAGTTGAGTTATGTTCAAGGTGGACAAGTTAAACCCGTCCTGCCTGAAAGGACGAGATCTCGATGGTACTTTGATGGTACTTCCTGTTCCAAAT

mRNA sequence

GGACAATGAATGCCATAAACAGCAAAAGTAACCCATTTTTTCTTTAATCTTAAGGCATCAGATTAGCAAATAGTAACTCAAGATTCTTCCCTTTTCTCTCTCTGAAAAACCCAATTGATGGATCCGCCTCTTTTCTAGAGAGAGAATTTGGGGCGCTGCTTTGACATATCTTCCAGGCTCCAGAATTCCCTTCACATGCGGTGAAGTTCTTATGGAGGGAGTGTTGCTTCAGTTGGCAAATTAGGGAAATCTTGCTCCCGATTGTTTATTTGGAGTTCTTCTTAAACAGCGGATGAGTAATTAAGAACTGCGAAGAACGTTCTTAAATTCATTTGCTGGTGGATCTTCGCTTATTTGCTGCCATCTGGGATTTGTGGATCGTTTGAAATTTTTTAACAAAATGGAGCATCGTGAGATGTTCGGTCCAGCTGAGGAAGATGATGTTGAAGATTTTCGAAGTTGTTGTGAAGATCATGAGGATGTGGTGGAAGAAGGGTCTGAGGAGGAGGAGGATCTTGATGAGTTTTCGGTGAAAATGTTCTTTAAGGGCATTTCGATAGCCGAGATTGGTGACCCGTCTTCTGGTCTTTCTGGTATTGGAGTGTTCATGGAAAGATCAGGGAAAGTCCCTTTTATTCAGGTCCAGAAAAAGCTTGATTTCTATGTTGATGAGCTAGTTGCTGATTATTTAGCTTTGATGGATGGATTGGTTGAGGCCATACAGAATGGCGTCCGCTCTATTCGAGCTTTTACCGACTCCAATACGTTATATAATCAGATAACAAATGAAGAGGAGCCTGAAATTCCGCTTCTTGTAGCGCTGAAAGAAAGGATTCTAGAGCATGCTAGCACTTTTGAAGCGTTTTCTCTGAACCTTTCGTCGCATTCAGACCTCGAGCAGCCATTTCATCTCGCGAAAGTGGCTATTGGGGTTCTTTCTTGTCCACAAAAGGGAGATAAATCAATTGAGAATTGTTCGATTTGTTGTGAGAACAAACCGACCGCTATGATGGTTGCGTTGAAGTGTTGTCATAAGTTCTGCTCTCATTGTATGAAGAGTTATGTTGATGGAAAATTAGAGACATCTCAAGTTCCTATCAGATGCCCTCAGCTGAGATGCAAATATTACATCACAAGCAATGAGTTTAAATCTTTTCTTCCCTTGACTTTATATGAATCGTTGGAGAATACCTTAGCCGAAGCGAATATTCACTCAGATAAAATTTACTGCCCTTATCCGAATTGCTCTGTTCTGTTAGACCCGAGCGAATGTTTGTCAGCTCGGGCAAGTTCATCAAGTCAATCGGATAACACTTGTATTGAATGTCCAGTTTGTCAGAGGTTTATTTGTGTTGAATGTTGTGTTCCTTGGCATTCATCAATGAGCTGTGAAGAGTTTCAGGACATCCCATTAGAGGAAAGAGACATTGCTGATATTACCTTACATCGTCTTGCACGAAACAAAAGGTGGCGACGTTGTCAGCAGTGTCGTAGGATGATTGAATTGGCTCAAGGCTGCTACCATATGACATGCTGGTGCGGGCATGAATTCTGCTATTCTTGTGGTGCTGAGTACCAAGATGGGCAACAGACCTGCCAATGCGCCTTTTGGGACGACGACGATGACGACGACAACTCCGAGGACTTGGTTACCCACTCCATCCAAGAATCTGAGCAGTGGGCATGGGAAACTTTTAATTCCCTCCCGATGATAACGGACGCATACTCAGAGCAAGAGAGATCACAGTTAGCACTAATCCAACGATTCCTGGCTGGGGGATTCAGTCTGAGTGACCACCAACCGTATCAATCGCCACCACGATGTACAGACTCTTATGTCGACACGATGAAGGACCTACACCAACTTCCATGGCTTGAGAGGTTCGTTTCCGTGATAAGTGATAACTACTATGAAGATTACGTACAATGATATATCGATTCTTAGACGAAAAATAAAAGGGGGAGGTAAAAGAGCCTCTCACTCTAGTTTGCCTCCCTCGATACCTCATTTACACCGCCGCACAGCTTGGCGTTTCGATCGCTAGTTACGCTCTGTTTGTCTGTTGAATCATATACTGGAAATTATGAAGTGACCCTCAGAGGTCCTCATTGCATTAGATTAGATTAGAATGAAGGAATCTTATGCAAGAATGTCAGCTTTGCTTGTTTGATTCTTTCCTGTTTCAAACAAAAGAAAAAGACAAGAAATAGTCAAGAATTTAATCAAAATGAAAATTATGTTCGAGTTGAGAGTATATGTTTCGATGAGTTGAGTTATGTTCAAGGTGGACAAGTTAAACCCGTCCTGCCTGAAAGGACGAGATCTCGATGGTACTTTGATGGTACTTCCTGTTCCAAAT

Coding sequence (CDS)

ATGGAGCATCGTGAGATGTTCGGTCCAGCTGAGGAAGATGATGTTGAAGATTTTCGAAGTTGTTGTGAAGATCATGAGGATGTGGTGGAAGAAGGGTCTGAGGAGGAGGAGGATCTTGATGAGTTTTCGGTGAAAATGTTCTTTAAGGGCATTTCGATAGCCGAGATTGGTGACCCGTCTTCTGGTCTTTCTGGTATTGGAGTGTTCATGGAAAGATCAGGGAAAGTCCCTTTTATTCAGGTCCAGAAAAAGCTTGATTTCTATGTTGATGAGCTAGTTGCTGATTATTTAGCTTTGATGGATGGATTGGTTGAGGCCATACAGAATGGCGTCCGCTCTATTCGAGCTTTTACCGACTCCAATACGTTATATAATCAGATAACAAATGAAGAGGAGCCTGAAATTCCGCTTCTTGTAGCGCTGAAAGAAAGGATTCTAGAGCATGCTAGCACTTTTGAAGCGTTTTCTCTGAACCTTTCGTCGCATTCAGACCTCGAGCAGCCATTTCATCTCGCGAAAGTGGCTATTGGGGTTCTTTCTTGTCCACAAAAGGGAGATAAATCAATTGAGAATTGTTCGATTTGTTGTGAGAACAAACCGACCGCTATGATGGTTGCGTTGAAGTGTTGTCATAAGTTCTGCTCTCATTGTATGAAGAGTTATGTTGATGGAAAATTAGAGACATCTCAAGTTCCTATCAGATGCCCTCAGCTGAGATGCAAATATTACATCACAAGCAATGAGTTTAAATCTTTTCTTCCCTTGACTTTATATGAATCGTTGGAGAATACCTTAGCCGAAGCGAATATTCACTCAGATAAAATTTACTGCCCTTATCCGAATTGCTCTGTTCTGTTAGACCCGAGCGAATGTTTGTCAGCTCGGGCAAGTTCATCAAGTCAATCGGATAACACTTGTATTGAATGTCCAGTTTGTCAGAGGTTTATTTGTGTTGAATGTTGTGTTCCTTGGCATTCATCAATGAGCTGTGAAGAGTTTCAGGACATCCCATTAGAGGAAAGAGACATTGCTGATATTACCTTACATCGTCTTGCACGAAACAAAAGGTGGCGACGTTGTCAGCAGTGTCGTAGGATGATTGAATTGGCTCAAGGCTGCTACCATATGACATGCTGGTGCGGGCATGAATTCTGCTATTCTTGTGGTGCTGAGTACCAAGATGGGCAACAGACCTGCCAATGCGCCTTTTGGGACGACGACGATGACGACGACAACTCCGAGGACTTGGTTACCCACTCCATCCAAGAATCTGAGCAGTGGGCATGGGAAACTTTTAATTCCCTCCCGATGATAACGGACGCATACTCAGAGCAAGAGAGATCACAGTTAGCACTAATCCAACGATTCCTGGCTGGGGGATTCAGTCTGAGTGACCACCAACCGTATCAATCGCCACCACGATGTACAGACTCTTATGTCGACACGATGAAGGACCTACACCAACTTCCATGGCTTGAGAGGTTCGTTTCCGTGATAAGTGATAACTACTATGAAGATTACGTACAATGA

Protein sequence

MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKGISIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLSCPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDDDDDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ
Homology
BLAST of CmaCh18G005450 vs. ExPASy Swiss-Prot
Match: Q8TC41 (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-17
Identity = 61/204 (29.90%), Postives = 92/204 (45.10%), Query Frame = 0

Query: 192 CSICCENKPTAMMVALKCCHK-FCSHCMKSYVDGKLETSQVPIRCPQLRCKYYITSNEFK 251
           C +C E+KP   +  L CC K  C  C+K Y+  +++  QV I+CP   C  ++   E  
Sbjct: 263 CRVCLEDKP---IKPLPCCKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFL--EETT 322

Query: 252 SFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSSQSDNTCIECP 311
               LT  +S++          D    P P C       +       S S+S    I+CP
Sbjct: 323 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYK-IQCP 382

Query: 312 VCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMIELA 371
            CQ   C +C  PWH  ++C+E++      R  A    H     +  ++C +C+  I+  
Sbjct: 383 TCQFVWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEH---GQRNAQKCPKCKIHIQRT 442

Query: 372 QGCYHMTC-WCGHEFCYSCGAEYQ 394
           +GC HMTC  C   FCY CG  Y+
Sbjct: 443 EGCDHMTCSQCNTNFCYRCGERYR 457

BLAST of CmaCh18G005450 vs. ExPASy Swiss-Prot
Match: D3YYI7 (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-17
Identity = 61/204 (29.90%), Postives = 92/204 (45.10%), Query Frame = 0

Query: 192 CSICCENKPTAMMVALKCCHK-FCSHCMKSYVDGKLETSQVPIRCPQLRCKYYITSNEFK 251
           C +C E+KP   +  L CC K  C  C+K Y+  +++  QV I+CP   C  ++   E  
Sbjct: 236 CRVCLEDKP---IKPLPCCKKAVCEECLKIYLSSQVQLGQVEIKCPVTECFEFL--EETT 295

Query: 252 SFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSSQSDNTCIECP 311
               LT  +S++          D    P P C       +       S S+S    I+CP
Sbjct: 296 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSES-RYKIQCP 355

Query: 312 VCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMIELA 371
            CQ   C +C  PWH  ++C+E++      R  A    H     +  ++C +C+  I+  
Sbjct: 356 TCQLIWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEH---GQRNAQKCPKCKIHIQRT 415

Query: 372 QGCYHMTC-WCGHEFCYSCGAEYQ 394
           +GC HMTC  C   FCY CG  Y+
Sbjct: 416 EGCDHMTCSQCNTNFCYRCGERYR 430

BLAST of CmaCh18G005450 vs. ExPASy Swiss-Prot
Match: Q9SKC3 (Probable E3 ubiquitin-protein ligase ARI9 OS=Arabidopsis thaliana OX=3702 GN=ARI9 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.9e-15
Identity = 55/214 (25.70%), Postives = 88/214 (41.12%), Query Frame = 0

Query: 192 CSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQ--VPIRCPQLRCKYYITSNEF 251
           C IC E+     +  + C H +C  C   Y+  K+E     + ++CP+  C   +  +  
Sbjct: 128 CGICFESYTREEIARVSCGHPYCKTCWAGYITTKIEDGPGCLRVKCPEPSCSAAVGKDMI 187

Query: 252 KSFLPLTLYESLENTLAEANIHSDK--IYCPYPNCSVLLDPSECLSARASSSSQSDNTCI 311
           +      + E     +  + +   K   +CP P C   ++         S SS  D +C+
Sbjct: 188 EDVTETKVNEKYSRYILRSYVEDGKKIKWCPSPGCGYAVE------FGGSESSSYDVSCL 247

Query: 312 ECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMI 371
               C    C  C    HS + C+       + +D ++     LA +K    C +C+R I
Sbjct: 248 ----CSYRFCWNCSEDAHSPVDCDTVSKWIFKNQDESENKNWMLANSK---PCPECKRPI 307

Query: 372 ELAQGCYHMTCW--CGHEFCYSCGAEYQDGQQTC 400
           E   GC HMTC   CGHEFC+ C   Y+     C
Sbjct: 308 EKNDGCNHMTCSAPCGHEFCWICLKAYRRHSGAC 328

BLAST of CmaCh18G005450 vs. ExPASy Swiss-Prot
Match: Q9SKC4 (Probable E3 ubiquitin-protein ligase ARI10 OS=Arabidopsis thaliana OX=3702 GN=ARI10 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 3.6e-14
Identity = 64/248 (25.81%), Postives = 96/248 (38.71%), Query Frame = 0

Query: 176 IGVLSCP---QKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQ-- 235
           +G+L  P     G +    C IC E+     + ++ C H +C  C   Y+  K+E     
Sbjct: 102 VGILKEPVVDVNGTEVDIQCGICFESYTRKEIASVSCGHPYCKTCWTGYITTKIEDGPGC 161

Query: 236 VPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDK--IYCPYPNCSVLLDP 295
           + ++CP+  C   +  +           +        + +   K   +CP P C      
Sbjct: 162 LRVKCPEPSCYAVVGQDMIDEVTEKKDKDKYYRYFLRSYVEDGKKMKWCPSPGC------ 221

Query: 296 SECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITL 355
            EC +     SS  D  C+    C    C  C    HS + CE       + +D ++   
Sbjct: 222 -EC-AVEFGESSGYDVACL----CSYRFCWNCSEDAHSPVDCETVSKWIFKNQDESE--- 281

Query: 356 HRLARNKRW-----RRCQQCRRMIELAQGCYHMTC--WCGHEFCYSCGAEYQDGQQTCQC 410
                NK W     + C +C+R IE + GC HMTC   CGH FC+ CG  Y D    C  
Sbjct: 282 -----NKNWILANSKPCPKCKRPIEKSHGCNHMTCSASCGHRFCWICGKSYSD-HYACNN 328

BLAST of CmaCh18G005450 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 4.7e-14
Identity = 62/244 (25.41%), Postives = 100/244 (40.98%), Query Frame = 0

Query: 191 NCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQ--VPIRCPQLRCKYYITSNE 250
           +C IC E+ P     AL C H++C  C K+Y++ K+      +   CP  +CK  +  + 
Sbjct: 138 SCLICLEDYPPTQTFALICNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVIVHQDA 197

Query: 251 FKSFLPLTLYESLENTLAEANIHSDK--IYCPYPNCSVLLDPSECLSARASSSSQSDNTC 310
           FK  +   ++E   N + ++ +  +    +CP P C          S R     + +   
Sbjct: 198 FKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAPGC--------IYSIRCDRKERKEAVN 257

Query: 311 IECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRM 370
            +C     F C +  +  H    C +      +  D ++     LA  K   +C +CR  
Sbjct: 258 CKCGFQYCFNCNDYEIGDHMPCPCSQVDKWLQKASDESENVTWMLANTK---KCPECRSP 317

Query: 371 IELAQGCYHMTC-----WCGHEFCYSCGAEYQDGQQTC----QCAFWDDD---DDDDNSE 419
           IE   GC HMTC      CG EFC+ C   + +   T      C  +D     +DDD + 
Sbjct: 318 IEKNGGCMHMTCRKNAGGCGFEFCWLCRGPWTEHGSTTGGYYNCNKYDKSKAKEDDDKAH 370

BLAST of CmaCh18G005450 vs. ExPASy TrEMBL
Match: A0A6J1K3V2 (probable E3 ubiquitin-protein ligase ARI11 OS=Cucurbita maxima OX=3661 GN=LOC111490412 PE=3 SV=1)

HSP 1 Score: 1056.2 bits (2730), Expect = 4.3e-305
Identity = 509/509 (100.00%), Postives = 509/509 (100.00%), Query Frame = 0

Query: 1   MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKGISIAEIGDPS 60
           MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKGISIAEIGDPS
Sbjct: 1   MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKGISIAEIGDPS 60

Query: 61  SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS 120
           SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS
Sbjct: 61  SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS 120

Query: 121 NTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS 180
           NTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS
Sbjct: 121 NTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS 180

Query: 181 CPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC 240
           CPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC
Sbjct: 181 CPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC 240

Query: 241 KYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS 300
           KYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS
Sbjct: 241 KYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS 300

Query: 301 QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC 360
           QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC
Sbjct: 301 QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC 360

Query: 361 QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDDDDDNSEDLVTHS 420
           QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDDDDDNSEDLVTHS
Sbjct: 361 QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDDDDDNSEDLVTHS 420

Query: 421 IQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYV 480
           IQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYV
Sbjct: 421 IQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYV 480

Query: 481 DTMKDLHQLPWLERFVSVISDNYYEDYVQ 510
           DTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 DTMKDLHQLPWLERFVSVISDNYYEDYVQ 509

BLAST of CmaCh18G005450 vs. ExPASy TrEMBL
Match: A0A6J1GTW7 (probable E3 ubiquitin-protein ligase ARI11 OS=Cucurbita moschata OX=3662 GN=LOC111456904 PE=3 SV=1)

HSP 1 Score: 1037.7 bits (2682), Expect = 1.6e-299
Identity = 501/512 (97.85%), Postives = 505/512 (98.63%), Query Frame = 0

Query: 1   MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKGISIAEIGDPS 60
           ME+REMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKG+SIAE+GDPS
Sbjct: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKGVSIAEVGDPS 60

Query: 61  SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS 120
           SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS
Sbjct: 61  SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS 120

Query: 121 NTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS 180
           NTLYNQITNEEEPEIPLLVALKERILEH STFEAFSLNLSSHSDLEQPFHLAKVAIGVLS
Sbjct: 121 NTLYNQITNEEEPEIPLLVALKERILEHTSTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS 180

Query: 181 CPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC 240
           CPQKGD S+ENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC
Sbjct: 181 CPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC 240

Query: 241 KYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS 300
           KYYITSNEFKSFLPLTLYE LENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS
Sbjct: 241 KYYITSNEFKSFLPLTLYELLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS 300

Query: 301 QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC 360
           QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC
Sbjct: 301 QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC 360

Query: 361 QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDD---DDDDDNSEDLV 420
           QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQD QQTCQCAFWDD   DDDDDNSEDLV
Sbjct: 361 QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDEQQTCQCAFWDDDDNDDDDDNSEDLV 420

Query: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480
           THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD
Sbjct: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480

Query: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 510
           SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 512

BLAST of CmaCh18G005450 vs. ExPASy TrEMBL
Match: A0A6J1CLL9 (uncharacterized protein LOC111012584 OS=Momordica charantia OX=3673 GN=LOC111012584 PE=3 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 1.0e-269
Identity = 457/521 (87.72%), Postives = 483/521 (92.71%), Query Frame = 0

Query: 1   MEHREMF------GPAEEDDVEDFRSCCEDH------EDVVEEGSEEEEDLDEFSVKMFF 60
           MEHRE+F      GPAEEDDVEDFRSCCEDH      ED+VEEGSEEEE LDEFSVKMFF
Sbjct: 1   MEHREIFGRGSSHGPAEEDDVEDFRSCCEDHEVWKEAEDLVEEGSEEEEHLDEFSVKMFF 60

Query: 61  KGISIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQ 120
           KGISIAEIGDP+SGLSGIGVFME+SGKVPFIQVQKKLDFYVDELVADYLALMDGLVEA+Q
Sbjct: 61  KGISIAEIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NGVRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQP 180
           N +R IRAFTDS+ LYNQITNEEE EIPLL+ALKERILEHASTFEAFSLNLSS +DLEQP
Sbjct: 121 NEIRCIRAFTDSDLLYNQITNEEESEIPLLIALKERILEHASTFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSCPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLET 240
           FHLAKVAIGVLSCPQKGDKS+ENCSICCENKP+AMMV LKCCHKFCSHCMK+YVDGK+++
Sbjct: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCENKPSAMMVGLKCCHKFCSHCMKTYVDGKVQS 240

Query: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDP 300
           SQVPIRCPQLRCKYYIT NEF+SFLPLTLYESLENTLAEANIHSD+IYCP+PNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITINEFRSFLPLTLYESLENTLAEANIHSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITL 360
           SECLS RASSSSQSDN+CIECPVCQRFICV C VPWHSSMSCEEFQ+I     D  DITL
Sbjct: 301 SECLSGRASSSSQSDNSCIECPVCQRFICVYCGVPWHSSMSCEEFQNI-----DTEDITL 360

Query: 361 HRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDD 420
           HRLA+NKRW+RCQQCRRMIEL QGCYHMTCWCGHEFCYSCGAEYQD QQTCQCA W    
Sbjct: 361 HRLAQNKRWKRCQQCRRMIELTQGCYHMTCWCGHEFCYSCGAEYQDSQQTCQCALW---- 420

Query: 421 DDDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQP 480
           D++NSEDLVTHSIQESEQWAWETFNSLPMI DAYSEQERSQLALIQRFLAGGFSLSDHQP
Sbjct: 421 DEENSEDLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP 480

Query: 481 YQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 510
           +QSPPRCTDSYVDTMKDLHQLPWLERFVSVISD+YYEDYVQ
Sbjct: 481 HQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDSYYEDYVQ 512

BLAST of CmaCh18G005450 vs. ExPASy TrEMBL
Match: A0A1S3C9E4 (E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 1.3e-269
Identity = 452/521 (86.76%), Postives = 485/521 (93.09%), Query Frame = 0

Query: 1   MEHREMF------GPAEEDDVEDFRSCCEDH------EDVVEEGSEEEEDLDEFSVKMFF 60
           MEHR++F      GPAEEDDVE+FRSCCEDH      ED VEE  E EEDLDEFSVKMFF
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEE--ESEEDLDEFSVKMFF 60

Query: 61  KGISIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQ 120
           KG+SIAEIGDP+SGLSGIGVFME+SGK+PF+QVQKKLDFYVDELVADYLALMDGLVEA+Q
Sbjct: 61  KGVSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NGVRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQP 180
           N VR IRAFTDS+ LY QITNEEEPEIPLLVALKERILEHAS FE+FSL LSS +DLEQP
Sbjct: 121 NDVRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADLEQP 180

Query: 181 FHLAKVAIGVLSCPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLET 240
           FHLAKVAIGVLSCPQKGDKS+ENCSICC+NKP+AMMVALKCCHKFCS C+K+YVDGK++ 
Sbjct: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGKVDA 240

Query: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDP 300
           SQVPIRCPQLRCKYYITSNEFKSFLPLTLYE LENTLAEANIH+D+IYCP+PNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITL 360
           SECLSARASSSSQSDN+CIECPVCQRFICVEC VPWHSS+SCEEFQ+IPLEERD ADITL
Sbjct: 301 SECLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITL 360

Query: 361 HRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDD 420
           HRLA+NKRWRRCQ+C RMIEL QGC+HMTCWCGHEFCYSCG EYQDGQQTCQCAFW    
Sbjct: 361 HRLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFW---- 420

Query: 421 DDDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQP 480
           D+DNSE+LVTHS+QESEQWAWETFNSLPMI DAYSEQERSQLALIQRFLAGGFSLSDHQP
Sbjct: 421 DEDNSENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP 480

Query: 481 YQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 510
           YQSPPRCTDSYVDTMKD+HQLPWLERFVSVISD+YYEDYVQ
Sbjct: 481 YQSPPRCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

BLAST of CmaCh18G005450 vs. ExPASy TrEMBL
Match: A0A5D3E1X1 (E3 ubiquitin-protein ligase arih1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G001170 PE=3 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 1.3e-269
Identity = 452/521 (86.76%), Postives = 485/521 (93.09%), Query Frame = 0

Query: 1   MEHREMF------GPAEEDDVEDFRSCCEDH------EDVVEEGSEEEEDLDEFSVKMFF 60
           MEHR++F      GPAEEDDVE+FRSCCEDH      ED VEE  E EEDLDEFSVKMFF
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEE--ESEEDLDEFSVKMFF 60

Query: 61  KGISIAEIGDPSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQ 120
           KG+SIAEIGDP+SGLSGIGVFME+SGK+PF+QVQKKLDFYVDELVADYLALMDGLVEA+Q
Sbjct: 61  KGVSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NGVRSIRAFTDSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQP 180
           N VR IRAFTDS+ LY QITNEEEPEIPLLVALKERILEHAS FE+FSL LSS +DLEQP
Sbjct: 121 NDVRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADLEQP 180

Query: 181 FHLAKVAIGVLSCPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLET 240
           FHLAKVAIGVLSCPQKGDKS+ENCSICC+NKP+AMMVALKCCHKFCS C+K+YVDGK++ 
Sbjct: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGKVDA 240

Query: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDP 300
           SQVPIRCPQLRCKYYITSNEFKSFLPLTLYE LENTLAEANIH+D+IYCP+PNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITL 360
           SECLSARASSSSQSDN+CIECPVCQRFICVEC VPWHSS+SCEEFQ+IPLEERD ADITL
Sbjct: 301 SECLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITL 360

Query: 361 HRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDD 420
           HRLA+NKRWRRCQ+C RMIEL QGC+HMTCWCGHEFCYSCG EYQDGQQTCQCAFW    
Sbjct: 361 HRLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFW---- 420

Query: 421 DDDNSEDLVTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQP 480
           D+DNSE+LVTHS+QESEQWAWETFNSLPMI DAYSEQERSQLALIQRFLAGGFSLSDHQP
Sbjct: 421 DEDNSENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQP 480

Query: 481 YQSPPRCTDSYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 510
           YQSPPRCTDSYVDTMKD+HQLPWLERFVSVISD+YYEDYVQ
Sbjct: 481 YQSPPRCTDSYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

BLAST of CmaCh18G005450 vs. NCBI nr
Match: XP_022994789.1 (probable E3 ubiquitin-protein ligase ARI11 [Cucurbita maxima])

HSP 1 Score: 1056.2 bits (2730), Expect = 8.9e-305
Identity = 509/509 (100.00%), Postives = 509/509 (100.00%), Query Frame = 0

Query: 1   MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKGISIAEIGDPS 60
           MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKGISIAEIGDPS
Sbjct: 1   MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKGISIAEIGDPS 60

Query: 61  SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS 120
           SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS
Sbjct: 61  SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS 120

Query: 121 NTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS 180
           NTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS
Sbjct: 121 NTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS 180

Query: 181 CPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC 240
           CPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC
Sbjct: 181 CPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC 240

Query: 241 KYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS 300
           KYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS
Sbjct: 241 KYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS 300

Query: 301 QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC 360
           QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC
Sbjct: 301 QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC 360

Query: 361 QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDDDDDNSEDLVTHS 420
           QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDDDDDNSEDLVTHS
Sbjct: 361 QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDDDDDNSEDLVTHS 420

Query: 421 IQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYV 480
           IQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYV
Sbjct: 421 IQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYV 480

Query: 481 DTMKDLHQLPWLERFVSVISDNYYEDYVQ 510
           DTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 DTMKDLHQLPWLERFVSVISDNYYEDYVQ 509

BLAST of CmaCh18G005450 vs. NCBI nr
Match: KAG6573449.1 (putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1042.7 bits (2695), Expect = 1.0e-300
Identity = 503/510 (98.63%), Postives = 507/510 (99.41%), Query Frame = 0

Query: 1   MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKGISIAEIGDPS 60
           ME+REMFGPAEEDDVEDFRSCCEDHEDVVEEGS+EEEDLDEFSVKMFFKG+SIAEIGDPS
Sbjct: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSKEEEDLDEFSVKMFFKGVSIAEIGDPS 60

Query: 61  SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS 120
           SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS
Sbjct: 61  SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS 120

Query: 121 NTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS 180
           NTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS
Sbjct: 121 NTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS 180

Query: 181 CPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC 240
           CPQKGD S+ENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC
Sbjct: 181 CPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC 240

Query: 241 KYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS 300
           KYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS
Sbjct: 241 KYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS 300

Query: 301 QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC 360
           QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC
Sbjct: 301 QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC 360

Query: 361 QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDD-DDDNSEDLVTH 420
           QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQC FWDDDD DDDNSEDLVTH
Sbjct: 361 QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDNDDDNSEDLVTH 420

Query: 421 SIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSY 480
           SIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSY
Sbjct: 421 SIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSY 480

Query: 481 VDTMKDLHQLPWLERFVSVISDNYYEDYVQ 510
           VDTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 VDTMKDLHQLPWLERFVSVISDNYYEDYVQ 510

BLAST of CmaCh18G005450 vs. NCBI nr
Match: KAG7012597.1 (putative E3 ubiquitin-protein ligase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1039.3 bits (2686), Expect = 1.1e-299
Identity = 504/512 (98.44%), Postives = 507/512 (99.02%), Query Frame = 0

Query: 1   MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGS--EEEEDLDEFSVKMFFKGISIAEIGD 60
           ME+REMFGPAEEDDVEDFRSCCEDHEDVVEEGS  EEEEDLDEFSVKMFFKG+SIAEIGD
Sbjct: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEEEDLDEFSVKMFFKGVSIAEIGD 60

Query: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120
           PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT
Sbjct: 61  PSSGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFT 120

Query: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180
           DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV
Sbjct: 121 DSNTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGV 180

Query: 181 LSCPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240
           LSCPQKGD S+ENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL
Sbjct: 181 LSCPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQL 240

Query: 241 RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300
           RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS
Sbjct: 241 RCKYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASS 300

Query: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360
           SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR
Sbjct: 301 SSQSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWR 360

Query: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDD-DDDNSEDLV 420
           RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQC FWDDDD DDDNSEDLV
Sbjct: 361 RCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCVFWDDDDNDDDNSEDLV 420

Query: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480
           THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD
Sbjct: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480

Query: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 510
           SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 512

BLAST of CmaCh18G005450 vs. NCBI nr
Match: XP_022954734.1 (probable E3 ubiquitin-protein ligase ARI11 [Cucurbita moschata])

HSP 1 Score: 1037.7 bits (2682), Expect = 3.3e-299
Identity = 501/512 (97.85%), Postives = 505/512 (98.63%), Query Frame = 0

Query: 1   MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKGISIAEIGDPS 60
           ME+REMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKG+SIAE+GDPS
Sbjct: 1   MENREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKGVSIAEVGDPS 60

Query: 61  SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS 120
           SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS
Sbjct: 61  SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS 120

Query: 121 NTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS 180
           NTLYNQITNEEEPEIPLLVALKERILEH STFEAFSLNLSSHSDLEQPFHLAKVAIGVLS
Sbjct: 121 NTLYNQITNEEEPEIPLLVALKERILEHTSTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS 180

Query: 181 CPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC 240
           CPQKGD S+ENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC
Sbjct: 181 CPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC 240

Query: 241 KYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS 300
           KYYITSNEFKSFLPLTLYE LENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS
Sbjct: 241 KYYITSNEFKSFLPLTLYELLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS 300

Query: 301 QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC 360
           QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC
Sbjct: 301 QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC 360

Query: 361 QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDD---DDDDDNSEDLV 420
           QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQD QQTCQCAFWDD   DDDDDNSEDLV
Sbjct: 361 QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDEQQTCQCAFWDDDDNDDDDDNSEDLV 420

Query: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480
           THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD
Sbjct: 421 THSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTD 480

Query: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 510
           SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 SYVDTMKDLHQLPWLERFVSVISDNYYEDYVQ 512

BLAST of CmaCh18G005450 vs. NCBI nr
Match: XP_023541881.1 (probable E3 ubiquitin-protein ligase ARI10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1029.6 bits (2661), Expect = 8.9e-297
Identity = 496/509 (97.45%), Postives = 501/509 (98.43%), Query Frame = 0

Query: 1   MEHREMFGPAEEDDVEDFRSCCEDHEDVVEEGSEEEEDLDEFSVKMFFKGISIAEIGDPS 60
           ME REMFGPAEEDDVEDFRSCCEDHEDVVE+GSEEE+DLDEFSVKMFFKG+SIAEIGDPS
Sbjct: 1   MERREMFGPAEEDDVEDFRSCCEDHEDVVEDGSEEEDDLDEFSVKMFFKGVSIAEIGDPS 60

Query: 61  SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS 120
           SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS
Sbjct: 61  SGLSGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDS 120

Query: 121 NTLYNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLS 180
           NTLYNQITNEEEPEIPLLVALKERILEHAST EAFSLNLSSHSDLEQPFHLAKVAIGVLS
Sbjct: 121 NTLYNQITNEEEPEIPLLVALKERILEHASTCEAFSLNLSSHSDLEQPFHLAKVAIGVLS 180

Query: 181 CPQKGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC 240
           CPQKGD S+ENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC
Sbjct: 181 CPQKGDTSVENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRC 240

Query: 241 KYYITSNEFKSFLPLTLYESLENTLAEANIHSDKIYCPYPNCSVLLDPSECLSARASSSS 300
           KYYITSNEFKSFLPLT YESLEN LAEANIHSDKIYCPYPNCS+LLDPSECLSARASSSS
Sbjct: 241 KYYITSNEFKSFLPLTSYESLENALAEANIHSDKIYCPYPNCSILLDPSECLSARASSSS 300

Query: 301 QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC 360
           QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC
Sbjct: 301 QSDNTCIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRC 360

Query: 361 QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDDDDDNSEDLVTHS 420
           QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFW   DDDDNSEDLVTHS
Sbjct: 361 QQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFW---DDDDNSEDLVTHS 420

Query: 421 IQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYV 480
           IQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYV
Sbjct: 421 IQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSYV 480

Query: 481 DTMKDLHQLPWLERFVSVISDNYYEDYVQ 510
           DTMKDLHQLPWLERFVSVISDNYYEDYVQ
Sbjct: 481 DTMKDLHQLPWLERFVSVISDNYYEDYVQ 506

BLAST of CmaCh18G005450 vs. TAIR 10
Match: AT4G19670.1 (RING/U-box superfamily protein )

HSP 1 Score: 653.3 bits (1684), Expect = 1.6e-187
Identity = 335/516 (64.92%), Postives = 407/516 (78.88%), Query Frame = 0

Query: 14  DVEDFRSCCEDHE----------DVVEEGSEEEEDLDEFSVKMFFKGISIAEIGDPSSGL 73
           D E+FRSCC D E           VVEE  +++ D DEFSVKM FKG+SI E GD  SG 
Sbjct: 20  DEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLD-DEFSVKMVFKGVSIFERGDLGSGY 79

Query: 74  SGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDSNTL 133
           SGIGV +ERSG +  IQVQKKLDFYV+E VA+YLALMDGL  A+QN + S+ A TDS  L
Sbjct: 80  SGIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEVALQNNLSSVVAVTDSELL 139

Query: 134 YNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLSCPQ 193
           YNQIT EE+ EIPLLVAL+ER+LE  S    F L L+   DL++   LA+VA+G++S   
Sbjct: 140 YNQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDLDEALSLAQVAVGIVSSNL 199

Query: 194 KGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRCKYY 253
            GDK IENCSICCE++ + MM++LKC HKFCSHCMK+YV+GK++TS+VPIRCPQ++CK+Y
Sbjct: 200 DGDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKHY 259

Query: 254 ITSNEFKSFLPLTLYESLENTLAEANI---HSDKIYCPYPNCSVLLDPSECLS---ARAS 313
           +++ E KSFLP+T ++S E    EAN+   ++ KIYCPYPNCS LLDP ECLS   A +S
Sbjct: 260 LSAAECKSFLPVTTFKSFE----EANVCSKNNGKIYCPYPNCSFLLDPQECLSSGRASSS 319

Query: 314 SSSQSDNT-CIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKR 373
           SS+QS+N+ C+ECPVC+RF+CV+C VPWH+SMSCEEFQ +P++ER   DITLHRLAR KR
Sbjct: 320 SSTQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKR 379

Query: 374 WRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDDDDDNSEDL 433
           WRRCQQCR MIELAQGC HMTC CGHEFCYSCGAEY++GQQTC CAFWDDD+++  +  +
Sbjct: 380 WRRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQTCTCAFWDDDEEEQENA-V 439

Query: 434 VTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLA-GGFSLSDHQ-PYQS-PP 493
             ++IQE EQW W+TFNS+P + DAYSEQERSQLALIQRFLA GGFSLSDH   YQS PP
Sbjct: 440 EENTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFLAGGGFSLSDHHTTYQSPPP 499

Query: 494 RCTD-SYVD-TMKDLHQLPWLERFVSVISDNYYEDY 508
            CT+ SYV+  MKDLHQLPWLERFVSVISD+YYE+Y
Sbjct: 500 PCTESSYVEAAMKDLHQLPWLERFVSVISDDYYEEY 529

BLAST of CmaCh18G005450 vs. TAIR 10
Match: AT4G19670.2 (RING/U-box superfamily protein )

HSP 1 Score: 653.3 bits (1684), Expect = 1.6e-187
Identity = 335/516 (64.92%), Postives = 407/516 (78.88%), Query Frame = 0

Query: 14  DVEDFRSCCEDHE----------DVVEEGSEEEEDLDEFSVKMFFKGISIAEIGDPSSGL 73
           D E+FRSCC D E           VVEE  +++ D DEFSVKM FKG+SI E GD  SG 
Sbjct: 20  DEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLD-DEFSVKMVFKGVSIFERGDLGSGY 79

Query: 74  SGIGVFMERSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDSNTL 133
           SGIGV +ERSG +  IQVQKKLDFYV+E VA+YLALMDGL  A+QN + S+ A TDS  L
Sbjct: 80  SGIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEVALQNNLSSVVAVTDSELL 139

Query: 134 YNQITNEEEPEIPLLVALKERILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLSCPQ 193
           YNQIT EE+ EIPLLVAL+ER+LE  S    F L L+   DL++   LA+VA+G++S   
Sbjct: 140 YNQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDLDEALSLAQVAVGIVSSNL 199

Query: 194 KGDKSIENCSICCENKPTAMMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRCKYY 253
            GDK IENCSICCE++ + MM++LKC HKFCSHCMK+YV+GK++TS+VPIRCPQ++CK+Y
Sbjct: 200 DGDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKHY 259

Query: 254 ITSNEFKSFLPLTLYESLENTLAEANI---HSDKIYCPYPNCSVLLDPSECLS---ARAS 313
           +++ E KSFLP+T ++S E    EAN+   ++ KIYCPYPNCS LLDP ECLS   A +S
Sbjct: 260 LSAAECKSFLPVTTFKSFE----EANVCSKNNGKIYCPYPNCSFLLDPQECLSSGRASSS 319

Query: 314 SSSQSDNT-CIECPVCQRFICVECCVPWHSSMSCEEFQDIPLEERDIADITLHRLARNKR 373
           SS+QS+N+ C+ECPVC+RF+CV+C VPWH+SMSCEEFQ +P++ER   DITLHRLAR KR
Sbjct: 320 SSTQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKR 379

Query: 374 WRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAEYQDGQQTCQCAFWDDDDDDDNSEDL 433
           WRRCQQCR MIELAQGC HMTC CGHEFCYSCGAEY++GQQTC CAFWDDD+++  +  +
Sbjct: 380 WRRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQTCTCAFWDDDEEEQENA-V 439

Query: 434 VTHSIQESEQWAWETFNSLPMITDAYSEQERSQLALIQRFLA-GGFSLSDHQ-PYQS-PP 493
             ++IQE EQW W+TFNS+P + DAYSEQERSQLALIQRFLA GGFSLSDH   YQS PP
Sbjct: 440 EENTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFLAGGGFSLSDHHTTYQSPPP 499

Query: 494 RCTD-SYVD-TMKDLHQLPWLERFVSVISDNYYEDY 508
            CT+ SYV+  MKDLHQLPWLERFVSVISD+YYE+Y
Sbjct: 500 PCTESSYVEAAMKDLHQLPWLERFVSVISDDYYEEY 529

BLAST of CmaCh18G005450 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 183.3 bits (464), Expect = 4.7e-46
Identity = 125/408 (30.64%), Postives = 206/408 (50.49%), Query Frame = 0

Query: 45  KMFFKGISIAE--IGDPSSGLSGIGV-FMERSGKVPFIQVQKKLDFYVDELVADYLALMD 104
           +++FKG+   E   G  +  +SG GV   ++   + F      +D  +    A+  AL+ 
Sbjct: 146 RLYFKGLVSDENGKGKMTDVVSGFGVAICDQRDNLLFEMKGPLIDNGMSRQGAELKALIR 205

Query: 105 GLVEAIQNGVRSIRAFTDSNTLYNQITNE---EEPEIPLLVALKERILEHASTFEAFSLN 164
           GL EA++ G++ I  F DS  ++  +T +   ++ +I LL+   + I++H S+++     
Sbjct: 206 GLTEALKLGIKHIVFFCDSYPIFQYVTGKWMAKQKKISLLLDDLQSIMQHFSSYQHV--- 265

Query: 165 LSSHSDLEQPFHLAKVAIGVLSCPQKGDKSI-----ENCSICCENKPTAMMVAL-KCCHK 224
           L + +D++  + LA+ +I  +  P +  +       E C+IC  +     M ++ KC H+
Sbjct: 266 LVARNDVKFAYKLARESILSMVTPHEDPRQAKAVLKEECAICFNDIVAEGMFSVDKCRHR 325

Query: 225 FCSHCMKSYVDGKLETSQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANIH- 284
           FC  C+K +V+ KL     P +CP   CK  +  +     L   L +  +  L E  I  
Sbjct: 326 FCFQCVKQHVEVKLLHGMAP-KCPHDGCKSELVIDACGKLLTPKLSKLWQQRLQENAIPV 385

Query: 285 SDKIYCPYPNCSVLLDPSECL-SARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSC 344
           ++++YCPYP CS L+  ++   SA++  S    +    C  C+   CV+C VPWH ++SC
Sbjct: 386 TERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVRRCVECRGLFCVDCKVPWHGNLSC 445

Query: 345 EEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGA 404
            E++ +   E    D+ L  LA NK WR+C +C+ MIEL+QGC H+TC CGHEFCY+CG 
Sbjct: 446 TEYKKLH-PEPPADDVKLKSLANNKMWRQCGKCQHMIELSQGCNHITCRCGHEFCYNCGG 505

Query: 405 EYQDGQQTC--QCAFW---------------------DDDDDDDNSED 416
            +     TC  +C  W                     DD+DDDD   D
Sbjct: 506 GWNKIMGTCLNRCPTWNEEYITRQDPARANVAANNYFDDEDDDDVDGD 548

BLAST of CmaCh18G005450 vs. TAIR 10
Match: AT2G25360.1 (RING/U-box superfamily protein )

HSP 1 Score: 173.7 bits (439), Expect = 3.8e-43
Identity = 112/331 (33.84%), Postives = 177/331 (53.47%), Query Frame = 0

Query: 97  LALMDGLVEAIQNGVRSIRAFTDSNTLYNQIT--NEEEPEIPLLVALKERILEHASTFEA 156
           +AL+ GL E+   G+R +  + D   LY  I    + + ++  LV   + +LE  +  +A
Sbjct: 1   MALIRGLRESFDLGIRQVVVYCDDQLLYQYIVGRGKIKKKVVHLVDEFQLLLEEMTYTDA 60

Query: 157 FSLNLSSHSDLEQPFHLAKVAIGVLSCPQKGDKSIENCSICCENKPTAMM--VALKCCHK 216
              +L + +D+   F LA+ AI       + D   E CSIC E      M     KC H+
Sbjct: 61  ---DLIALNDVNFAFKLAREAI-----VSRDDVKAEICSICFEETEGERMFFTTEKCVHR 120

Query: 217 FCSHCMKSYVDGKLETSQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLENTLAEANI-H 276
            C  C+K YV+ KL +  VP  C    CK+ +T       L L L E  +  + E +I  
Sbjct: 121 HCFPCVKQYVEVKLLSGTVP-TCLDDGCKFKLTLESCSKVLTLELIEMWKQKMKEDSIPA 180

Query: 277 SDKIYCPYPNCSVLLDPSECLSARASSSSQSDNTCIECPVCQRFICVECCVPWHSSMSCE 336
           +++IYCPYPNCS+L+  +E  S    S   +D +C++C  C  F C++C VP HS +SC 
Sbjct: 181 AERIYCPYPNCSMLMSKTELSS---ESDLSNDRSCVKC--CGLF-CIDCKVPSHSDLSCA 240

Query: 337 EFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMIELAQGCYHMTCWCGHEFCYSCGAE 396
           E++ +   +  + ++ L  LA++K+WR+C+ CR MIEL+  C HMTC CG++FCY C  E
Sbjct: 241 EYKKLH-HDPLVDELKLKSLAKDKKWRQCKMCRHMIELSHACNHMTCRCGYQFCYQCEVE 300

Query: 397 YQDGQQTCQC-------AFWDDDDDDDNSED 416
           +++ Q+TC          ++DD D ++   D
Sbjct: 301 WKNDQKTCSSGCLLTGHGYYDDYDYNEPEYD 315

BLAST of CmaCh18G005450 vs. TAIR 10
Match: AT2G25370.1 (RING/U-box superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 8.4e-43
Identity = 120/372 (32.26%), Postives = 196/372 (52.69%), Query Frame = 0

Query: 30  EEGSEEEEDLDEFSVKMFFKG-ISIAEIGD--PSSGLSGIGVFMERSGKVPFIQVQKKL- 89
           E  S   + +D    ++ FKG ++   + D    +  +G GV +         ++++ L 
Sbjct: 140 EGSSPAAKFMDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHEIKESLR 199

Query: 90  DFYVDELVADYLALMDGLVEAIQNGVRSIRAFTDSNTLYNQI--TNEEEPEIPLLVALKE 149
           D  +     + +AL+ GL E+   G+R++  + D + +Y  I    + + +I  LV   +
Sbjct: 200 DIEISRRGVEIMALVRGLSESFDLGMRNVVIYCDDDWIYQSIIGRGKSKKKIDHLVEEVQ 259

Query: 150 RILEHASTFEAFSLNLSSHSDLEQPFHLAKVAIGVLSCPQKGDKSIENCSICCENKPTA- 209
            ILE  +  +A    L + +D++  F LA+ AIG  S     ++  E C I  E      
Sbjct: 260 GILEKMACIDAV---LVARNDVKFAFRLAREAIGRNSVDVNAEQG-ETCGIFFEETDVEH 319

Query: 210 MMVALKCCHKFCSHCMKSYVDGKLETSQVPIRCPQLRCKYYITSNEFKSFLPLTLYESLE 269
           M V  KC H+ C  C+K +V  KL +   P  C +  CK+ +T       L L L E  +
Sbjct: 320 MFVTEKCLHRHCFPCVKQHVKVKLRSGTEP-TCLEYGCKFKLTLERCSKVLTLKLIEMWK 379

Query: 270 NTLAEANI-HSDKIYCPYPNCSVLLDPSECLSARASSSSQSDNTCIECPVCQRFICVECC 329
             + E +I  +++IYCPYPNCS+L+  +E LS+ A  S+    TC++C  C  F C++C 
Sbjct: 380 QKMKEDSIPAAERIYCPYPNCSMLMSKTE-LSSEADLSNV--RTCVKC--CGLF-CIDCK 439

Query: 330 VPWHSSMSCEEFQDIPLEERDIADITLHRLARNKRWRRCQQCRRMIELAQGCYHMTCWCG 389
           VP H+ +S ++++ +  +   + D+ L  LA +K WR+C +CR MIEL+ GC HMTC CG
Sbjct: 440 VPSHTDLSYDDYKKLHPDPL-VDDLKLKSLANDKMWRQCVKCRHMIELSHGCNHMTCRCG 499

Query: 390 HEFCYSCGAEYQ 394
           +EFCY CG E+Q
Sbjct: 500 YEFCYECGIEWQ 499

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8TC411.2e-1729.90Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE... [more]
D3YYI71.2e-1729.90Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 P... [more]
Q9SKC31.9e-1525.70Probable E3 ubiquitin-protein ligase ARI9 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q9SKC43.6e-1425.81Probable E3 ubiquitin-protein ligase ARI10 OS=Arabidopsis thaliana OX=3702 GN=AR... [more]
Q6T4864.7e-1425.41Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 G... [more]
Match NameE-valueIdentityDescription
A0A6J1K3V24.3e-305100.00probable E3 ubiquitin-protein ligase ARI11 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1GTW71.6e-29997.85probable E3 ubiquitin-protein ligase ARI11 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1CLL91.0e-26987.72uncharacterized protein LOC111012584 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
A0A1S3C9E41.3e-26986.76E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 S... [more]
A0A5D3E1X11.3e-26986.76E3 ubiquitin-protein ligase arih1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
XP_022994789.18.9e-305100.00probable E3 ubiquitin-protein ligase ARI11 [Cucurbita maxima][more]
KAG6573449.11.0e-30098.63putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sor... [more]
KAG7012597.11.1e-29998.44putative E3 ubiquitin-protein ligase [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_022954734.13.3e-29997.85probable E3 ubiquitin-protein ligase ARI11 [Cucurbita moschata][more]
XP_023541881.18.9e-29797.45probable E3 ubiquitin-protein ligase ARI10 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G19670.11.6e-18764.92RING/U-box superfamily protein [more]
AT4G19670.21.6e-18764.92RING/U-box superfamily protein [more]
AT5G60250.14.7e-4630.64zinc finger (C3HC4-type RING finger) family protein [more]
AT2G25360.13.8e-4333.84RING/U-box superfamily protein [more]
AT2G25370.18.4e-4332.26RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002867IBR domainSMARTSM00647ibrneu5coord: 340..399
e-value: 0.087
score: 13.6
coord: 259..330
e-value: 7.0E-9
score: 45.5
IPR002867IBR domainPFAMPF01485IBRcoord: 351..399
e-value: 4.9E-6
score: 26.7
coord: 268..330
e-value: 6.6E-8
score: 32.7
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 360..401
e-value: 11.0
score: 2.3
coord: 192..238
e-value: 0.0039
score: 26.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 192..239
score: 10.792683
NoneNo IPR availableGENE3D1.20.120.1750coord: 328..458
e-value: 1.3E-14
score: 56.7
NoneNo IPR availablePANTHERPTHR11685:SF353RBR-TYPE E3 UBIQUITIN TRANSFERASEcoord: 13..508
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 343..407
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 187..266
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 62..159
e-value: 3.4E-10
score: 39.8
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 186..273
e-value: 4.2E-14
score: 54.2
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 44..178
e-value: 7.4E-8
score: 34.4
IPR031127E3 ubiquitin ligase RBR familyPANTHERPTHR11685RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAININGcoord: 13..508
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 209..218
IPR044066TRIAD supradomainPROSITEPS51873TRIADcoord: 188..405
score: 29.162672
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 60..162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G005450.1CmaCh18G005450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000151 ubiquitin ligase complex
molecular_function GO:0004386 helicase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity