CmaCh18G000350 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh18G000350
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	protein ASPARTIC PROTEASE IN GUARD CELL 1-like
Location	Cma_Chr18: 135761 .. 137239 (+)
RNA-Seq Expression	CmaCh18G000350
Synteny	CmaCh18G000350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCTGCAAAGCCAAATCAGCTACCTTTCTGTCCTTTCGCCATCTTCCTCTTCCTCTTCCTCTTCCTCTCTCTCTTCCCCTTCACTCTCTCCTCCTCCGCTTCACTCGATGTTTCTACTTCTCTGAAACAAGCCCACCAGGTCCTTAAATTCGACCCTGAGATTTTGACCTCTTTCCACCAGCAGGAACTGCTTCTTCCGGCGAACTCTTCTTCTTCCTCCTCCTTCTCTCTGCAGCTGCACCCTCGTGATGCTCTTCGCAATGCCGGACATACGGACTACAGGAGCTTGGTCCTCTCCCGGCTCGACCGTGACTCGTCTAGAGTTAAATCGCTTAATGATAGGCTTGAATTTGGCTTAAGCGACTTGACTAGGTCGGATCTTCAGCCGTTGAACGCGGACATTCTGCCGGAGGATCTGAGCACTCCCATTGTCTCTGGCTCCAGTCAGGGGAGTGGCGAGTACTTCTCCCGCGTTGGCGTTGGTCAACCGGCCAAGCCATTCTATATGGTTCTGGATACCGGCAGTGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGTTACCAGCAAACCGACCCCATTTTTGACCCTACCACTTCGTCTTCTTTTACTTCCCTTCCCTGCGAATCTCCGCACTGTCAAGCTCTTGAAATGTCTGGTTGTCGTTCTGCGAAGTGTCTTTACCAGGTCTCGTACGGCGATGGCTCCTTCACCGTCGGCCAGTTCGTCACTGAAACGCTGTCGTTTGGTAACTCCGGAGCGATTAACAACGTCGCTCTCGGCTGTGGCCACGACAACGAAGGACTGTTCATCGGATCCGCCGGATTGCTTGGTCTCGGCGGCGGATCACTTTCCCTAACTTCTCAGATGAAAGCTTCGTCGTTTTCGTACTGTCTTGTCGACCGCGACTCTGGTTCAGCATCAACTCTCGAGTTCAATTCCGCTGCACCGAATGACTCGGTGAATGCTCAATTACTCAGAAGCGGAAGAGTCAATACATTCTACTACGTTGCACTCACCGGCTTGAGCGTCGGCGGCCATCCACTCTCTATTCCGCCTTCTCTGTTTCAATTGGACGATTCCGGCCACGGCGGCATCATCGTAGATTCAGGAACCGCCATAACTAGACTGCAGACTCAGGCCTACAACTCCCTTCGCGACGCCTTCGTGAGGCTCACACCGTACTTGAAGAAGGCGAACAGCTTCGCATTATTCGACACATGTTACGATCTGTCGTCGCAATCGAGAGTGACGATTCCGACAGTATCTTTTCATTTCTCGGGCGGAAAGTCGCTGCAGCTGGCGCCGAAGAACTACCTCATTCCGGTGGATTCAGCCGGAACTTTCTGTTTTGCGTTCGCCCCAACGACGTCGTCGCTGTCCATCATAGGGAACGTTCAGCAGCAAGGGACACGTGTCAACTTCGACTTGGCTAATTCGGTGGTAGCATTTTCACCCAATAAATGTTAG

mRNA sequence

ATGGCGTCTGCAAAGCCAAATCAGCTACCTTTCTGTCCTTTCGCCATCTTCCTCTTCCTCTTCCTCTTCCTCTCTCTCTTCCCCTTCACTCTCTCCTCCTCCGCTTCACTCGATGTTTCTACTTCTCTGAAACAAGCCCACCAGGTCCTTAAATTCGACCCTGAGATTTTGACCTCTTTCCACCAGCAGGAACTGCTTCTTCCGGCGAACTCTTCTTCTTCCTCCTCCTTCTCTCTGCAGCTGCACCCTCGTGATGCTCTTCGCAATGCCGGACATACGGACTACAGGAGCTTGGTCCTCTCCCGGCTCGACCGTGACTCGTCTAGAGTTAAATCGCTTAATGATAGGCTTGAATTTGGCTTAAGCGACTTGACTAGGTCGGATCTTCAGCCGTTGAACGCGGACATTCTGCCGGAGGATCTGAGCACTCCCATTGTCTCTGGCTCCAGTCAGGGGAGTGGCGAGTACTTCTCCCGCGTTGGCGTTGGTCAACCGGCCAAGCCATTCTATATGGTTCTGGATACCGGCAGTGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGTTACCAGCAAACCGACCCCATTTTTGACCCTACCACTTCGTCTTCTTTTACTTCCCTTCCCTGCGAATCTCCGCACTGTCAAGCTCTTGAAATGTCTGGTTGTCGTTCTGCGAAGTGTCTTTACCAGGTCTCGTACGGCGATGGCTCCTTCACCGTCGGCCAGTTCGTCACTGAAACGCTGTCGTTTGGTAACTCCGGAGCGATTAACAACGTCGCTCTCGGCTGTGGCCACGACAACGAAGGACTGTTCATCGGATCCGCCGGATTGCTTGGTCTCGGCGGCGGATCACTTTCCCTAACTTCTCAGATGAAAGCTTCGTCGTTTTCGTACTGTCTTGTCGACCGCGACTCTGGTTCAGCATCAACTCTCGAGTTCAATTCCGCTGCACCGAATGACTCGGTGAATGCTCAATTACTCAGAAGCGGAAGAGTCAATACATTCTACTACGTTGCACTCACCGGCTTGAGCGTCGGCGGCCATCCACTCTCTATTCCGCCTTCTCTGTTTCAATTGGACGATTCCGGCCACGGCGGCATCATCGTAGATTCAGGAACCGCCATAACTAGACTGCAGACTCAGGCCTACAACTCCCTTCGCGACGCCTTCGTGAGGCTCACACCGTACTTGAAGAAGGCGAACAGCTTCGCATTATTCGACACATGTTACGATCTGTCGTCGCAATCGAGAGTGACGATTCCGACAGTATCTTTTCATTTCTCGGGCGGAAAGTCGCTGCAGCTGGCGCCGAAGAACTACCTCATTCCGGTGGATTCAGCCGGAACTTTCTGTTTTGCGTTCGCCCCAACGACGTCGTCGCTGTCCATCATAGGGAACGTTCAGCAGCAAGGGACACGTGTCAACTTCGACTTGGCTAATTCGGTGGTAGCATTTTCACCCAATAAATGTTAG

Coding sequence (CDS)

ATGGCGTCTGCAAAGCCAAATCAGCTACCTTTCTGTCCTTTCGCCATCTTCCTCTTCCTCTTCCTCTTCCTCTCTCTCTTCCCCTTCACTCTCTCCTCCTCCGCTTCACTCGATGTTTCTACTTCTCTGAAACAAGCCCACCAGGTCCTTAAATTCGACCCTGAGATTTTGACCTCTTTCCACCAGCAGGAACTGCTTCTTCCGGCGAACTCTTCTTCTTCCTCCTCCTTCTCTCTGCAGCTGCACCCTCGTGATGCTCTTCGCAATGCCGGACATACGGACTACAGGAGCTTGGTCCTCTCCCGGCTCGACCGTGACTCGTCTAGAGTTAAATCGCTTAATGATAGGCTTGAATTTGGCTTAAGCGACTTGACTAGGTCGGATCTTCAGCCGTTGAACGCGGACATTCTGCCGGAGGATCTGAGCACTCCCATTGTCTCTGGCTCCAGTCAGGGGAGTGGCGAGTACTTCTCCCGCGTTGGCGTTGGTCAACCGGCCAAGCCATTCTATATGGTTCTGGATACCGGCAGTGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGTTACCAGCAAACCGACCCCATTTTTGACCCTACCACTTCGTCTTCTTTTACTTCCCTTCCCTGCGAATCTCCGCACTGTCAAGCTCTTGAAATGTCTGGTTGTCGTTCTGCGAAGTGTCTTTACCAGGTCTCGTACGGCGATGGCTCCTTCACCGTCGGCCAGTTCGTCACTGAAACGCTGTCGTTTGGTAACTCCGGAGCGATTAACAACGTCGCTCTCGGCTGTGGCCACGACAACGAAGGACTGTTCATCGGATCCGCCGGATTGCTTGGTCTCGGCGGCGGATCACTTTCCCTAACTTCTCAGATGAAAGCTTCGTCGTTTTCGTACTGTCTTGTCGACCGCGACTCTGGTTCAGCATCAACTCTCGAGTTCAATTCCGCTGCACCGAATGACTCGGTGAATGCTCAATTACTCAGAAGCGGAAGAGTCAATACATTCTACTACGTTGCACTCACCGGCTTGAGCGTCGGCGGCCATCCACTCTCTATTCCGCCTTCTCTGTTTCAATTGGACGATTCCGGCCACGGCGGCATCATCGTAGATTCAGGAACCGCCATAACTAGACTGCAGACTCAGGCCTACAACTCCCTTCGCGACGCCTTCGTGAGGCTCACACCGTACTTGAAGAAGGCGAACAGCTTCGCATTATTCGACACATGTTACGATCTGTCGTCGCAATCGAGAGTGACGATTCCGACAGTATCTTTTCATTTCTCGGGCGGAAAGTCGCTGCAGCTGGCGCCGAAGAACTACCTCATTCCGGTGGATTCAGCCGGAACTTTCTGTTTTGCGTTCGCCCCAACGACGTCGTCGCTGTCCATCATAGGGAACGTTCAGCAGCAAGGGACACGTGTCAACTTCGACTTGGCTAATTCGGTGGTAGCATTTTCACCCAATAAATGTTAG

Protein sequence

MASAKPNQLPFCPFAIFLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPNKC

Homology

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BLAST of CmaCh18G000350 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 2.6e-163
Identity = 298/497 (59.96%), Postives = 373/497 (75.05%), Query Frame = 0


Query: 13  PFAIFLFLFLFLSLFPFTLSSSAS----------LDVSTSLKQAHQVLKFDP--EILTSF 72
           P  + L   + LSLF  T  +S+           LDV +SL+Q   +L  DP    LT+ 
Sbjct: 4   PRFLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTT 63

Query: 73  HQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFG 132
             + L  P   +SSS  SL+LH RD    + H DY+SL LSRL+RDSSRV  +  ++ F 
Sbjct: 64  KPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFA 123

Query: 133 LSDLTRSDLQPL-NAD--ILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGS 192
           +  + RSDL+P+ N D     EDL+TP+VSG+SQGSGEYFSR+GVG PAK  Y+VLDTGS
Sbjct: 124 VEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGS 183

Query: 193 DINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGD 252
           D+NW+QC+PC DCYQQ+DP+F+PT+SS++ SL C +P C  LE S CRS KCLYQVSYGD
Sbjct: 184 DVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGD 243

Query: 253 GSFTVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASS 312
           GSFTVG+  T+T++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG LS+T+QMKA+S
Sbjct: 244 GSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATS 303

Query: 313 FSYCLVDRDSGSASTLEFNSA-APNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPP 372
           FSYCLVDRDSG +S+L+FNS         A LLR+ +++TFYYV L+G SVGG  + +P 
Sbjct: 304 FSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPD 363

Query: 373 SLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKK-ANSFALFDTCYDLS 432
           ++F +D SG GG+I+D GTA+TRLQTQAYNSLRDAF++LT  LKK ++S +LFDTCYD S
Sbjct: 364 AIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFS 423

Query: 433 SQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 492
           S S V +PTV+FHF+GGKSL L  KNYLIPVD +GTFCFAFAPT+SSLSIIGNVQQQGTR
Sbjct: 424 SLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTR 483

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BLAST of CmaCh18G000350 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 9.8e-110
Identity = 240/488 (49.18%), Postives = 306/488 (62.70%), Query Frame = 0


Query: 16  IFLFLFLFLSLFPF-TLSSSASLDVSTSLKQAHQVLKFDPEI-LTSFHQQELLLPANSSS 75
           +F   F FLSL  F +L S  +L  ++        + F P+    S  + E    ++S S
Sbjct: 9   LFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDSES 68

Query: 76  SSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLND-RLEFGLSDLTRSDLQPL 135
           SSS +L L   DAL +    D   L  SRL RDS RVKS+     +    ++T +     
Sbjct: 69  SSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAP---- 128

Query: 136 NADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 195
                P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQC PC  CY 
Sbjct: 129 ----RPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYS 188

Query: 196 QTDPIFDPTTSSSFTSLPCESPHCQALEMSGC--RSAKCLYQVSYGDGSFTVGQFVTETL 255
           Q+DPIFDP  S ++ ++PC SPHC+ L+ +GC  R   CLYQVSYGDGSFTVG F TETL
Sbjct: 189 QSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETL 248

Query: 256 SFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSL---TSQMKASSFSYCLVDRDS 315
           +F     +  VALGCGHDNEGLF+G+AGLLGLG G LS    T       FSYCLVDR +
Sbjct: 249 TF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSA 308

Query: 316 GS--ASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPL-SIPPSLFQLDDS 375
            S  +S +  N+A    +    LL + +++TFYYV L G+SVGG  +  +  SLF+LD  
Sbjct: 309 SSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQI 368

Query: 376 GHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPT 435
           G+GG+I+DSGT++TRL   AY ++RDAF      LK+A  F+LFDTC+DLS+ + V +PT
Sbjct: 369 GNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPT 428

Query: 436 VSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSV 493
           V  HF G   + L   NYLIPVD+ G FCFAFA T   LSIIGN+QQQG RV +DLA+S 
Sbjct: 429 VVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSR 484

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BLAST of CmaCh18G000350 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 3.0e-103
Identity = 206/480 (42.92%), Postives = 288/480 (60.00%), Query Frame = 0


Query: 17  FLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLPANSSSSSS 76
           F FL L L L   +  S     +   L+    V    P+   +    E        SSS 
Sbjct: 7   FFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDE--------SSSK 66

Query: 77  FSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSDLQPLNADI 136
           ++L+L  RD   +  + ++   + +R+ RD+ RV ++  R+   +   + S  +      
Sbjct: 67  YTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYE------ 126

Query: 137 LPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDP 196
              D  + IVSG  QGSGEYF R+GVG P +  YMV+D+GSD+ W+QCQPC  CY+Q+DP
Sbjct: 127 -VNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDP 186

Query: 197 IFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETLSFGNSG 256
           +FDP  S S+T + C S  C  +E SGC S  C Y+V YGDGS+T G    ETL+F  + 
Sbjct: 187 VFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKT- 246

Query: 257 AINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMK---ASSFSYCLVDRDSGSASTL 316
            + NVA+GCGH N G+FIG+AGLLG+GGGS+S   Q+      +F YCLV R + S  +L
Sbjct: 247 VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSL 306

Query: 317 EF-NSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGIIVD 376
            F   A P  +    L+R+ R  +FYYV L GL VGG  + +P  +F L ++G GG+++D
Sbjct: 307 VFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMD 366

Query: 377 SGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFHFSGG 436
           +GTA+TRL T AY + RD F   T  L +A+  ++FDTCYDLS    V +PTVSF+F+ G
Sbjct: 367 TGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEG 426

Query: 437 KSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPNKC 493
             L L  +N+L+PVD +GT+CFAFA + + LSIIGN+QQ+G +V+FD AN  V F PN C
Sbjct: 427 PVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

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BLAST of CmaCh18G000350 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.7e-74
Identity = 156/358 (43.58%), Postives = 206/358 (57.54%), Query Frame = 0


Query: 141 LSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDP 200
           + TP+ +    G GEY   V +G P   F  ++DTGSD+ W QC+PCT C+ Q  PIF+P
Sbjct: 85  IETPVYA----GDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNP 144

Query: 201 TTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETLSFGNSGAINN 260
             SSSF++LPCES +CQ L    C + +C Y   YGDGS T G   TET +F  S ++ N
Sbjct: 145 QDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETS-SVPN 204

Query: 261 VALGCGHDNEGLFIGS-AGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSASTLEFNSAA 320
           +A GCG DN+G   G+ AGL+G+G G LSL SQ+    FSYC+    S S STL   SAA
Sbjct: 205 IAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAA 264

Query: 321 ---PNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGIIVDSGTA 380
              P  S +  L+ S    T+YY+ L G++VGG  L IP S FQL D G GG+I+DSGT 
Sbjct: 265 SGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTT 324

Query: 381 ITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQ-SRVTIPTVSFHFSGGKSL 440
           +T L   AYN++  AF           S +   TC+   S  S V +P +S  F GG  L
Sbjct: 325 LTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGG-VL 384

Query: 441 QLAPKNYLIPVDSAGTFCFAFAPTTS-SLSIIGNVQQQGTRVNFDLANSVVAFSPNKC 493
            L  +N LI   + G  C A   ++   +SI GN+QQQ T+V +DL N  V+F P +C
Sbjct: 385 NLGEQNILIS-PAEGVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

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BLAST of CmaCh18G000350 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 6.6e-74
Identity = 156/347 (44.96%), Postives = 206/347 (59.37%), Query Frame = 0


Query: 152 GSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPC 211
           G GEY   + +G PA+PF  ++DTGSD+ W QCQPCT C+ Q+ PIF+P  SSSF++LPC
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPC 150

Query: 212 ESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETLSFGNSGAINNVALGCGHDNEG 271
            S  CQAL    C +  C Y   YGDGS T G   TETL+FG S +I N+  GCG +N+G
Sbjct: 151 SSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFG-SVSIPNITFGCGENNQG 210

Query: 272 LFIGS-AGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSASTLEFNSAAPN---DSVNAQ 331
              G+ AGL+G+G G LSL SQ+  + FSYC+    S + S L   S A +    S N  
Sbjct: 211 FGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTT 270

Query: 332 LLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLD-DSGHGGIIVDSGTAITRLQTQAYN 391
           L++S ++ TFYY+ L GLSVG   L I PS F L+ ++G GGII+DSGT +T     AY 
Sbjct: 271 LIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQ 330

Query: 392 SLRDAFVRLTPYLKKANSFALFDTCYDL-SSQSRVTIPTVSFHFSGGKSLQLAPKNYLIP 451
           S+R  F+          S + FD C+   S  S + IPT   HF GG  L+L  +NY I 
Sbjct: 331 SVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELPSENYFIS 390

Query: 452 VDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPNKC 493
             S G  C A   ++  +SI GN+QQQ   V +D  NSVV+F+  +C
Sbjct: 391 -PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

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BLAST of CmaCh18G000350 vs. ExPASy TrEMBL
Match: A0A6J1JXD0 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111490538 PE=3 SV=1)

HSP 1 Score: 971.1 bits (2509), Expect = 1.8e-279
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0


Query: 1   MASAKPNQLPFCPFAIFLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSF 60
           MASAKPNQLPFCPFAIFLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSF
Sbjct: 1   MASAKPNQLPFCPFAIFLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSF 60

Query: 61  HQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFG 120
           HQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFG
Sbjct: 61  HQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFG 120

Query: 121 LSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDIN 180
           LSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDIN
Sbjct: 121 LSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDIN 180

Query: 181 WLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSF 240
           WLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSF
Sbjct: 181 WLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSF 240

Query: 241 TVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSY 300
           TVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSY
Sbjct: 241 TVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSY 300

Query: 301 CLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQ 360
           CLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQ
Sbjct: 301 CLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQ 360

Query: 361 LDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRV 420
           LDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRV
Sbjct: 361 LDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRV 420

Query: 421 TIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDL 480
           TIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDL
Sbjct: 421 TIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDL 480

Query: 481 ANSVVAFSPNKC 493
           ANSVVAFSPNKC
Sbjct: 481 ANSVVAFSPNKC 492

[Close]

BLAST of CmaCh18G000350 vs. ExPASy TrEMBL
Match: A0A6J1GS20 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456998 PE=3 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.0e-266
Identity = 477/497 (95.98%), Postives = 482/497 (96.98%), Query Frame = 0


Query: 1   MASAKPNQLPFCPFAIFLFLFLFLSLFPFTL-----SSSASLDVSTSLKQAHQVLKFDPE 60
           MASA+PNQLPF PFAI  FLFLFLSLFPFTL     SSSASLDVSTSLKQAHQVLKF P+
Sbjct: 1   MASARPNQLPFFPFAI--FLFLFLSLFPFTLSSSSSSSSASLDVSTSLKQAHQVLKFGPK 60

Query: 61  ILTSFHQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLND 120
           ILTSFHQQELL+PAN SSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLND
Sbjct: 61  ILTSFHQQELLVPAN-SSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLND 120

Query: 121 RLEFGLSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDT 180
           RLEFG +DLTRSDLQPLN DILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDT
Sbjct: 121 RLEFGSTDLTRSDLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDT 180

Query: 181 GSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSY 240
           GSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCR+AKCLYQVSY
Sbjct: 181 GSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSY 240

Query: 241 GDGSFTVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKA 300
           GDGSFTVGQFVTETLSFGNSG INNVALGCGHDNEGLFIGSAGLLGLGGGSLSL SQMKA
Sbjct: 241 GDGSFTVGQFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLNSQMKA 300

Query: 301 SSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIP 360
           SSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIP
Sbjct: 301 SSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIP 360

Query: 361 PSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLS 420
           PSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLS
Sbjct: 361 PSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLS 420

Query: 421 SQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 480
           SQSRVTIPTVSFHFSGGKSLQL PKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR
Sbjct: 421 SQSRVTIPTVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 480

Query: 481 VNFDLANSVVAFSPNKC 493
           VNFDLANSVVAFSPNKC
Sbjct: 481 VNFDLANSVVAFSPNKC 494

[Close]

BLAST of CmaCh18G000350 vs. ExPASy TrEMBL
Match: A0A6J1C6K9 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Momordica charantia OX=3673 GN=LOC111008878 PE=3 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.6e-232
Identity = 417/482 (86.51%), Postives = 437/482 (90.66%), Query Frame = 0


Query: 20  LFLFLSLFPFT---------LSSSASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLPAN 79
           +F+F    PFT          SSSA LDVS SLKQA QVLKFDP+ L SF QQE L+PAN
Sbjct: 8   IFIFSLFLPFTSCRSSPELSRSSSALLDVSASLKQADQVLKFDPKGLMSFQQQEQLVPAN 67

Query: 80  SSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSDLQ 139
              SSSFSLQLHPRDALRNAGH DY+SLVLSRLDRDSSRVKSLNDRL+F LSDL RSDLQ
Sbjct: 68  ---SSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRSDLQ 127

Query: 140 PLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDC 199
           PL  +ILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDC
Sbjct: 128 PLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDC 187

Query: 200 YQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETL 259
           YQQ+DPIFDP TSSSF SLPCES  CQALEMSGCR+AKCLYQVSYGDGSFTVG+FVTETL
Sbjct: 188 YQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVTETL 247

Query: 260 SFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSA 319
           SFGNSG INNVALGCGHDNEGLF+GSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGS+
Sbjct: 248 SFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSS 307

Query: 320 STLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGII 379
           S+L+FNS AP+DSV A LLRSGRV+TFYYV LTGLSVGG  LSIPPSLFQ+DDSG+GGII
Sbjct: 308 SSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYGGII 367

Query: 380 VDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFHFS 439
           VDSGTAITRLQTQAYNSLRDAFVRLTPYL+K N FALFDTCYDLSSQSRVTIPTVSFHFS
Sbjct: 368 VDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSFHFS 427

Query: 440 GGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPN 493
           GGKSL L PKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS+V FSPN
Sbjct: 428 GGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGFSPN 486

[Close]

BLAST of CmaCh18G000350 vs. ExPASy TrEMBL
Match: A0A6J1E815 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111431581 PE=3 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.7e-227
Identity = 410/500 (82.00%), Postives = 439/500 (87.80%), Query Frame = 0


Query: 4   AKPNQLPFCPFAIFLFLFLFLSLFPFTL-----------SSSASLDVSTSLKQAHQVLKF 63
           A  N LP   FA F     F SL PFTL           SSSA LDVS SLKQA+QVLKF
Sbjct: 2   ATANLLPLFSFASF-----FFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKF 61

Query: 64  DPEILTSFHQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKS 123
           DP+ L SF QQ+ ++P N  SSSSFSLQLH RDAL+NAGH DY+SLVLSRLDRDSSRVKS
Sbjct: 62  DPKALLSFQQQDEVVPVN--SSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKS 121

Query: 124 LNDRLEFGLSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMV 183
           LNDRLEF LSDL RSDLQPL  +ILPEDLSTPIVSG+SQGSGEYFSR+GVG+PAKPFYMV
Sbjct: 122 LNDRLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMV 181

Query: 184 LDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQ 243
           LDTGSDINWLQCQPCTDCYQQTDPIFDP TSSSF SLPCESP CQALE SGCR+AKCLYQ
Sbjct: 182 LDTGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQ 241

Query: 244 VSYGDGSFTVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQ 303
           V+YGDGSFTVG+FVTE+LSFGNSG INNVALGCGHDNEGLF+GSAGLLGLGGGSLSLTSQ
Sbjct: 242 VAYGDGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQ 301

Query: 304 MKASSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPL 363
           MKASSFSYCLVDRDS S+S LEFNSAAP+DSVNA LLRSGRV+TFYYV L GLSVGG  L
Sbjct: 302 MKASSFSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLL 361

Query: 364 SIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCY 423
           SIPPSLFQ+DD+G+GGIIVDSGTAITRLQTQAYN+LRDAFV  TPYL++ N FALFDTCY
Sbjct: 362 SIPPSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCY 421

Query: 424 DLSSQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQ 483
           DLSSQSRVTIPT+SF F+GGKSLQL PKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQ
Sbjct: 422 DLSSQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQ 481

Query: 484 GTRVNFDLANSVVAFSPNKC 493
           GTRV+FDLANS+V FSPNKC
Sbjct: 482 GTRVHFDLANSLVGFSPNKC 494

[Close]

BLAST of CmaCh18G000350 vs. ExPASy TrEMBL
Match: A0A6J1KJJ8 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111495193 PE=3 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 9.5e-225
Identity = 407/501 (81.24%), Postives = 436/501 (87.03%), Query Frame = 0


Query: 4   AKPNQLPFCPFAIFLFLFLFLSLFPFTL------------SSSASLDVSTSLKQAHQVLK 63
           A  N  P   FAIF     F SL PFTL            SSSA LDVS SLKQA+QVLK
Sbjct: 2   ATANLQPLFSFAIF-----FFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLK 61

Query: 64  FDPEILTSFHQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVK 123
           FDP+ L SF QQ  ++PAN  SSSSFSLQLH RDAL+NAGH DY+SLVLSRLDRDSSRVK
Sbjct: 62  FDPKALLSFQQQNEVVPAN--SSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVK 121

Query: 124 SLNDRLEFGLSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYM 183
           SLNDRLEF LSDL RSDLQPL  +ILPEDLSTPIVSG+SQGSGEYFSR+GVGQPAKPFYM
Sbjct: 122 SLNDRLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYM 181

Query: 184 VLDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLY 243
           VLDTGSDINWLQCQPCTDCYQQTDPIFDP TSSSF SL CESP CQALE SGCR+AKCLY
Sbjct: 182 VLDTGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLY 241

Query: 244 QVSYGDGSFTVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTS 303
           QV+YGDGSFTVG+FVTE+LSFGNSG INNVALGCGHDNEGLF+GSAGLLGLGGGSLSL S
Sbjct: 242 QVAYGDGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLIS 301

Query: 304 QMKASSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHP 363
           QMKASSFSYCLVDRDS S+S LEFNSAAP+D+VNA LLRSGRV+TFYYV L GLSVGG  
Sbjct: 302 QMKASSFSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQL 361

Query: 364 LSIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTC 423
           LSIPPSLFQ+DD+G+GGIIVDSGTAITRLQTQAYN+LRDAFV  TPYL++ N  ALFDTC
Sbjct: 362 LSIPPSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTC 421

Query: 424 YDLSSQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQ 483
           YDLSSQSRVTIPT+SF F+GGKSLQL PKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQ
Sbjct: 422 YDLSSQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQ 481

Query: 484 QGTRVNFDLANSVVAFSPNKC 493
           QGTRV+FDLANS++ FSPNKC
Sbjct: 482 QGTRVHFDLANSLLGFSPNKC 495

[Close]

BLAST of CmaCh18G000350 vs. NCBI nr
Match: XP_022994952.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima])

HSP 1 Score: 971.1 bits (2509), Expect = 3.6e-279
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0


Query: 1   MASAKPNQLPFCPFAIFLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSF 60
           MASAKPNQLPFCPFAIFLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSF
Sbjct: 1   MASAKPNQLPFCPFAIFLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSF 60

Query: 61  HQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFG 120
           HQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFG
Sbjct: 61  HQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFG 120

Query: 121 LSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDIN 180
           LSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDIN
Sbjct: 121 LSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDIN 180

Query: 181 WLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSF 240
           WLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSF
Sbjct: 181 WLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSF 240

Query: 241 TVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSY 300
           TVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSY
Sbjct: 241 TVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSY 300

Query: 301 CLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQ 360
           CLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQ
Sbjct: 301 CLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQ 360

Query: 361 LDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRV 420
           LDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRV
Sbjct: 361 LDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRV 420

Query: 421 TIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDL 480
           TIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDL
Sbjct: 421 TIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDL 480

Query: 481 ANSVVAFSPNKC 493
           ANSVVAFSPNKC
Sbjct: 481 ANSVVAFSPNKC 492

[Close]

BLAST of CmaCh18G000350 vs. NCBI nr
Match: KAG6572933.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 935.3 bits (2416), Expect = 2.2e-268
Identity = 479/502 (95.42%), Postives = 484/502 (96.41%), Query Frame = 0


Query: 1   MASAKPNQLPFCPFAIFLFLFLFLSLFPFTL----------SSSASLDVSTSLKQAHQVL 60
           MASA+PNQLPF PFAI    FLFLSLFPFTL          SSSASLDVSTSLKQAHQVL
Sbjct: 1   MASARPNQLPFFPFAI----FLFLSLFPFTLSSSSSSSSSSSSSASLDVSTSLKQAHQVL 60

Query: 61  KFDPEILTSFHQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRV 120
           KFDP+ILTSFHQQELL+PA+SSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRV
Sbjct: 61  KFDPKILTSFHQQELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRV 120

Query: 121 KSLNDRLEFGLSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFY 180
           KSLNDRLEFGLSDLTRSDLQPLN DILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFY
Sbjct: 121 KSLNDRLEFGLSDLTRSDLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFY 180

Query: 181 MVLDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCL 240
           MVLDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCR+AKCL
Sbjct: 181 MVLDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCL 240

Query: 241 YQVSYGDGSFTVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLT 300
           YQVSYGDGSFTVGQFVTETLSFGNSG INNVALGCGHDNEGLFIGSAGLLGLGGGSLSLT
Sbjct: 241 YQVSYGDGSFTVGQFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLT 300

Query: 301 SQMKASSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGH 360
           SQMKASSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGH
Sbjct: 301 SQMKASSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGH 360

Query: 361 PLSIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDT 420
           PLSIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDT
Sbjct: 361 PLSIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDT 420

Query: 421 CYDLSSQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQ 480
           CYDLSSQSRVTIPTVSFHFSGGKSLQL PKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQ
Sbjct: 421 CYDLSSQSRVTIPTVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQ 480

Query: 481 QQGTRVNFDLANSVVAFSPNKC 493
           QQGTRVNFDLANSVVAFSPNKC
Sbjct: 481 QQGTRVNFDLANSVVAFSPNKC 498

[Close]

BLAST of CmaCh18G000350 vs. NCBI nr
Match: XP_022954867.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata])

HSP 1 Score: 928.7 bits (2399), Expect = 2.1e-266
Identity = 477/497 (95.98%), Postives = 482/497 (96.98%), Query Frame = 0


Query: 1   MASAKPNQLPFCPFAIFLFLFLFLSLFPFTL-----SSSASLDVSTSLKQAHQVLKFDPE 60
           MASA+PNQLPF PFAI  FLFLFLSLFPFTL     SSSASLDVSTSLKQAHQVLKF P+
Sbjct: 1   MASARPNQLPFFPFAI--FLFLFLSLFPFTLSSSSSSSSASLDVSTSLKQAHQVLKFGPK 60

Query: 61  ILTSFHQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLND 120
           ILTSFHQQELL+PAN SSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLND
Sbjct: 61  ILTSFHQQELLVPAN-SSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLND 120

Query: 121 RLEFGLSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDT 180
           RLEFG +DLTRSDLQPLN DILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDT
Sbjct: 121 RLEFGSTDLTRSDLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDT 180

Query: 181 GSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSY 240
           GSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCR+AKCLYQVSY
Sbjct: 181 GSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSY 240

Query: 241 GDGSFTVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKA 300
           GDGSFTVGQFVTETLSFGNSG INNVALGCGHDNEGLFIGSAGLLGLGGGSLSL SQMKA
Sbjct: 241 GDGSFTVGQFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLNSQMKA 300

Query: 301 SSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIP 360
           SSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIP
Sbjct: 301 SSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIP 360

Query: 361 PSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLS 420
           PSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLS
Sbjct: 361 PSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLS 420

Query: 421 SQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 480
           SQSRVTIPTVSFHFSGGKSLQL PKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR
Sbjct: 421 SQSRVTIPTVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 480

Query: 481 VNFDLANSVVAFSPNKC 493
           VNFDLANSVVAFSPNKC
Sbjct: 481 VNFDLANSVVAFSPNKC 494

[Close]

BLAST of CmaCh18G000350 vs. NCBI nr
Match: XP_023541582.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 922.5 bits (2383), Expect = 1.5e-264
Identity = 472/500 (94.40%), Postives = 478/500 (95.60%), Query Frame = 0


Query: 1   MASAKPNQLPFCPFAIFLFLFLFLSLFPFTLS--------SSASLDVSTSLKQAHQVLKF 60
           MASA PNQLPFCPFAI     LFLSLFPFTLS        SSA LDVSTSLKQAHQVLKF
Sbjct: 1   MASANPNQLPFCPFAI----ILFLSLFPFTLSRTSPELSHSSALLDVSTSLKQAHQVLKF 60

Query: 61  DPEILTSFHQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKS 120
           DP+ILTSFH Q+LL+P NSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKS
Sbjct: 61  DPKILTSFHHQQLLVPPNSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKS 120

Query: 121 LNDRLEFGLSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMV 180
           LNDRLEFGLSDLTRSDLQPLN DILPEDLSTPIVSGSSQGSGEYFSRVGVG+PAKPFYMV
Sbjct: 121 LNDRLEFGLSDLTRSDLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGRPAKPFYMV 180

Query: 181 LDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQ 240
           LDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSL CESPHCQALEMSGCR+AKCLYQ
Sbjct: 181 LDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLTCESPHCQALEMSGCRAAKCLYQ 240

Query: 241 VSYGDGSFTVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQ 300
           VSYGDGSFTVGQFVTETLSFGNSG INNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQ
Sbjct: 241 VSYGDGSFTVGQFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQ 300

Query: 301 MKASSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPL 360
           MKASSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGR+NTFYYVALTGLSVGGHPL
Sbjct: 301 MKASSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRLNTFYYVALTGLSVGGHPL 360

Query: 361 SIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCY 420
           SIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAY SLRDAFVRLTPYLKKANSFALFDTCY
Sbjct: 361 SIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYISLRDAFVRLTPYLKKANSFALFDTCY 420

Query: 421 DLSSQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQ 480
           DLSSQSRVTIPTVSFHFSGGKSLQL PKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQ
Sbjct: 421 DLSSQSRVTIPTVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQ 480

Query: 481 GTRVNFDLANSVVAFSPNKC 493
           GTRVNFDLANSVVAFSPNKC
Sbjct: 481 GTRVNFDLANSVVAFSPNKC 496

[Close]

BLAST of CmaCh18G000350 vs. NCBI nr
Match: KAG7012115.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 841.6 bits (2173), Expect = 3.3e-240
Identity = 422/429 (98.37%), Postives = 425/429 (99.07%), Query Frame = 0


Query: 64  ELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 123
           ELL+PA+SSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD
Sbjct: 1   ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 60

Query: 124 LTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 183
           LTRS LQPLN DILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ
Sbjct: 61  LTRSGLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 120

Query: 184 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVG 243
           CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCR+AKCLYQVSYGDGSFTVG
Sbjct: 121 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 180

Query: 244 QFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 303
           QFVTETLSFGNSG INNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV
Sbjct: 181 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 240

Query: 304 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 363
           DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD
Sbjct: 241 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 300

Query: 364 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 423
           SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP
Sbjct: 301 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 360

Query: 424 TVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 483
           TVSFHFSGGKSLQL PKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS
Sbjct: 361 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 420

Query: 484 VVAFSPNKC 493
           VVAFSPNKC
Sbjct: 421 VVAFSPNKC 429

[Close]

BLAST of CmaCh18G000350 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 576.6 bits (1485), Expect = 1.9e-164
Identity = 298/497 (59.96%), Postives = 373/497 (75.05%), Query Frame = 0


Query: 13  PFAIFLFLFLFLSLFPFTLSSSAS----------LDVSTSLKQAHQVLKFDP--EILTSF 72
           P  + L   + LSLF  T  +S+           LDV +SL+Q   +L  DP    LT+ 
Sbjct: 4   PRFLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTT 63

Query: 73  HQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFG 132
             + L  P   +SSS  SL+LH RD    + H DY+SL LSRL+RDSSRV  +  ++ F 
Sbjct: 64  KPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFA 123

Query: 133 LSDLTRSDLQPL-NAD--ILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGS 192
           +  + RSDL+P+ N D     EDL+TP+VSG+SQGSGEYFSR+GVG PAK  Y+VLDTGS
Sbjct: 124 VEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGS 183

Query: 193 DINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGD 252
           D+NW+QC+PC DCYQQ+DP+F+PT+SS++ SL C +P C  LE S CRS KCLYQVSYGD
Sbjct: 184 DVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGD 243

Query: 253 GSFTVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASS 312
           GSFTVG+  T+T++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG LS+T+QMKA+S
Sbjct: 244 GSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATS 303

Query: 313 FSYCLVDRDSGSASTLEFNSA-APNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPP 372
           FSYCLVDRDSG +S+L+FNS         A LLR+ +++TFYYV L+G SVGG  + +P 
Sbjct: 304 FSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPD 363

Query: 373 SLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKK-ANSFALFDTCYDLS 432
           ++F +D SG GG+I+D GTA+TRLQTQAYNSLRDAF++LT  LKK ++S +LFDTCYD S
Sbjct: 364 AIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFS 423

Query: 433 SQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 492
           S S V +PTV+FHF+GGKSL L  KNYLIPVD +GTFCFAFAPT+SSLSIIGNVQQQGTR
Sbjct: 424 SLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTR 483

[Close]

BLAST of CmaCh18G000350 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 551.2 bits (1419), Expect = 8.4e-157
Identity = 279/489 (57.06%), Postives = 372/489 (76.07%), Query Frame = 0


Query: 14  FAIFLFLF-------LFLSLFPFTLSSSAS-LDVSTSLKQAHQVLKFDPEILTSFHQQEL 73
           ++ F F+F       +F  + P T +++ S L+V+ S+ +      F        +QQE 
Sbjct: 5   YSFFFFIFFLTSHSSVFSRILPETSTTTTSILNVADSIHRTKYTSSF------RLNQQE- 64

Query: 74  LLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLT 133
                 S+SSSFSLQLH R ++R   H+DY+SL L+RL+RD++RVKSL  RL+  +++++
Sbjct: 65  --EQTHSASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNIS 124

Query: 134 RSDLQPLNADILPE--DLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 193
           ++DL+P++     E  D+  P++SG++QGSGEYF+RVG+G+PA+  YMVLDTGSD+NWLQ
Sbjct: 125 KADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQ 184

Query: 194 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVG 253
           C PC DCY QT+PIF+P++SSS+  L C++P C ALE+S CR+A CLY+VSYGDGS+TVG
Sbjct: 185 CTPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVG 244

Query: 254 QFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 313
            F TETL+ G S  + NVA+GCGH NEGLF+G+AGLLGLGGG L+L SQ+  +SFSYCLV
Sbjct: 245 DFATETLTIG-STLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLV 304

Query: 314 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 373
           DRDS SAST++F ++   D+V A LLR+ +++TFYY+ LTG+SVGG  L IP S F++D+
Sbjct: 305 DRDSDSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDE 364

Query: 374 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 433
           SG GGII+DSGTA+TRLQT+ YNSLRD+FV+ T  L+KA   A+FDTCY+LS+++ V +P
Sbjct: 365 SGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVP 424

Query: 434 TVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 493
           TV+FHF GGK L L  KNY+IPVDS GTFC AFAPT SSL+IIGNVQQQGTRV FDLANS
Sbjct: 425 TVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANS 483

[Close]

BLAST of CmaCh18G000350 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 398.7 bits (1023), Expect = 6.9e-111
Identity = 240/488 (49.18%), Postives = 306/488 (62.70%), Query Frame = 0


Query: 16  IFLFLFLFLSLFPF-TLSSSASLDVSTSLKQAHQVLKFDPEI-LTSFHQQELLLPANSSS 75
           +F   F FLSL  F +L S  +L  ++        + F P+    S  + E    ++S S
Sbjct: 9   LFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDSES 68

Query: 76  SSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLND-RLEFGLSDLTRSDLQPL 135
           SSS +L L   DAL +    D   L  SRL RDS RVKS+     +    ++T +     
Sbjct: 69  SSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAP---- 128

Query: 136 NADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 195
                P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQC PC  CY 
Sbjct: 129 ----RPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYS 188

Query: 196 QTDPIFDPTTSSSFTSLPCESPHCQALEMSGC--RSAKCLYQVSYGDGSFTVGQFVTETL 255
           Q+DPIFDP  S ++ ++PC SPHC+ L+ +GC  R   CLYQVSYGDGSFTVG F TETL
Sbjct: 189 QSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETL 248

Query: 256 SFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSL---TSQMKASSFSYCLVDRDS 315
           +F     +  VALGCGHDNEGLF+G+AGLLGLG G LS    T       FSYCLVDR +
Sbjct: 249 TF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSA 308

Query: 316 GS--ASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPL-SIPPSLFQLDDS 375
            S  +S +  N+A    +    LL + +++TFYYV L G+SVGG  +  +  SLF+LD  
Sbjct: 309 SSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQI 368

Query: 376 GHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPT 435
           G+GG+I+DSGT++TRL   AY ++RDAF      LK+A  F+LFDTC+DLS+ + V +PT
Sbjct: 369 GNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPT 428

Query: 436 VSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSV 493
           V  HF G   + L   NYLIPVD+ G FCFAFA T   LSIIGN+QQQG RV +DLA+S 
Sbjct: 429 VVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSR 484

[Close]

BLAST of CmaCh18G000350 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 384.4 bits (986), Expect = 1.4e-106
Identity = 240/501 (47.90%), Postives = 301/501 (60.08%), Query Frame = 0


Query: 7   NQLPFCPFAIFLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSFHQQELL 66
           N L F  FA+  F            +SSAS    T +     V         S+ + E L
Sbjct: 7   NTLAFSVFAVLFF------------TSSASSQYQTLV-----VNTLPSSATLSWPESESL 66

Query: 67  LPAN-SSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLT 126
              + S S++S S+ L   DAL +        L   RL RDS RVKS+       L+ ++
Sbjct: 67  TDESLSESTTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSIT-----SLAAVS 126

Query: 127 RSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQ 186
                           S  ++SG SQGSGEYF R+GVG PA   YMVLDTGSD+ WLQC 
Sbjct: 127 TGRNATKRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCS 186

Query: 187 PCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQAL-EMSGC---RSAKCLYQVSYGDGSFT 246
           PC  CY QTD IFDP  S +F ++PC S  C+ L + S C   RS  CLYQVSYGDGSFT
Sbjct: 187 PCKACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFT 246

Query: 247 VGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMK---ASSF 306
            G F TETL+F +   +++V LGCGHDNEGLF+G+AGLLGLG G LS  SQ K      F
Sbjct: 247 EGDFSTETLTF-HGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKF 306

Query: 307 SYCLVDR-DSGSA----STLEF-NSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPL 366
           SYCLVDR  SGS+    ST+ F N+A P  SV   LL + +++TFYY+ L G+SVGG  +
Sbjct: 307 SYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRV 366

Query: 367 -SIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTC 426
             +  S F+LD +G+GG+I+DSGT++TRL   AY +LRDAF      LK+A S++LFDTC
Sbjct: 367 PGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTC 426

Query: 427 YDLSSQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQ 486
           +DLS  + V +PTV FHF GG+ + L   NYLIPV++ G FCFAFA T  SLSIIGN+QQ
Sbjct: 427 FDLSGMTTVKVPTVVFHFGGGE-VSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQ 483

Query: 487 QGTRVNFDLANSVVAFSPNKC 493
           QG RV +DL  S V F    C
Sbjct: 487 QGFRVAYDLVGSRVGFLSRAC 483

[Close]

BLAST of CmaCh18G000350 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 377.1 bits (967), Expect = 2.2e-104
Identity = 206/480 (42.92%), Postives = 288/480 (60.00%), Query Frame = 0


Query: 17  FLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLPANSSSSSS 76
           F FL L L L   +  S     +   L+    V    P+   +    E        SSS 
Sbjct: 7   FFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDE--------SSSK 66

Query: 77  FSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSDLQPLNADI 136
           ++L+L  RD   +  + ++   + +R+ RD+ RV ++  R+   +   + S  +      
Sbjct: 67  YTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYE------ 126

Query: 137 LPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDP 196
              D  + IVSG  QGSGEYF R+GVG P +  YMV+D+GSD+ W+QCQPC  CY+Q+DP
Sbjct: 127 -VNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDP 186

Query: 197 IFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETLSFGNSG 256
           +FDP  S S+T + C S  C  +E SGC S  C Y+V YGDGS+T G    ETL+F  + 
Sbjct: 187 VFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKT- 246

Query: 257 AINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMK---ASSFSYCLVDRDSGSASTL 316
            + NVA+GCGH N G+FIG+AGLLG+GGGS+S   Q+      +F YCLV R + S  +L
Sbjct: 247 VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSL 306

Query: 317 EF-NSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGIIVD 376
            F   A P  +    L+R+ R  +FYYV L GL VGG  + +P  +F L ++G GG+++D
Sbjct: 307 VFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMD 366

Query: 377 SGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFHFSGG 436
           +GTA+TRL T AY + RD F   T  L +A+  ++FDTCYDLS    V +PTVSF+F+ G
Sbjct: 367 TGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEG 426

Query: 437 KSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPNKC 493
             L L  +N+L+PVD +GT+CFAFA + + LSIIGN+QQ+G +V+FD AN  V F PN C
Sbjct: 427 PVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

The following BLAST results are available for this feature:

BLAST of CmaCh18G000350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LS40	2.6e-163	59.96	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	9.8e-110	49.18	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LHE3	3.0e-103	42.92	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q766C2	1.7e-74	43.58	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q766C3	6.6e-74	44.96	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]

BLAST of CmaCh18G000350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JXD0	1.8e-279	100.00	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1GS20	1.0e-266	95.98	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1C6K9	1.6e-232	86.51	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1E815	2.7e-227	82.00	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1KJJ8	9.5e-225	81.24	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of CmaCh18G000350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022994952.1	3.6e-279	100.00	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima]	[more]
KAG6572933.1	2.2e-268	95.42	Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_022954867.1	2.1e-266	95.98	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata]	[more]
XP_023541582.1	1.5e-264	94.40	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG7012115.1	3.3e-240	98.37	Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp...	[more]

BLAST of CmaCh18G000350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18490.1	1.9e-164	59.96	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	8.4e-157	57.06	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	6.9e-111	49.18	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	1.4e-106	47.90	Eukaryotic aspartyl protease family protein	[more]
AT3G20015.1	2.2e-104	42.92	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 156..315 e-value: 1.5E-47 score: 162.1
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 337..488 e-value: 6.7E-38 score: 130.0
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 133..311 e-value: 3.0E-50 score: 172.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 312..492 e-value: 2.0E-51 score: 176.3
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 150..492
None	No IPR available	PANTHER	PTHR13683:SF274	PROTEIN ASPARTIC PROTEASE IN GUARD CELL 1	coord: 30..492
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 30..492
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 171..182
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 156..488 score: 45.262596

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh18G000350.1	CmaCh18G000350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity