CmaCh17G012140 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G012140
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionprotein OSB2, chloroplastic-like
LocationCma_Chr17: 8385674 .. 8391369 (-)
RNA-Seq ExpressionCmaCh17G012140
SyntenyCmaCh17G012140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCGCACACAAAGGAACACAGTCGAAGATAAGAGGGAAATCGAGAGCGATGATAATGGCGGTAGAGAAAACCCTAACTCTTTCGTCGTCATTTTTATCAAAGAACTGCATCTTCTCCTTCTCTAGAAACAAGTCACGAGTCGCTTCCTTCTCCCATTTTCCCTCGCATCTGAACGCTGCTAAACACAGCCGTTTCGCTACTCGTTTCCTAAAATGCTTGGCTATGGAAAACTACAGCATTTACTCTGTGTCCTACCCCAAACCTTCCGAAATTCCATGGAAGAAGGAGCTCAGTAACTCGGTCAGTCTCATCGGGACAGTTGGTACGCCCGTGGAGCTCAAGCACCTACCATCGGGCAAAGATGTCGCGCGGACGCGACTTGCTGTGTGGAAGTCATCTACTGAAACATCTTGGTACTAATCTAATGTTCTTTCCGAAATGGGTTTTTATGGTTACATTTTATAGAATATTGAATGATAATGTGATTTTTATTTTCGGGGGGATGCTTATGAGGATTAAATTATTTTCGAATATGAAGAATAAGCTCTTGTGTAGTTTGGAGTTGAGATGTTAAGAATATGGAAGTTTTAGGACCGTGGTTATAGACTATATTTCTGATTACGGGGGAGTGGCATCCCAAAATTGTTGTCTCAATTTCAGTAGAATTTGGAAACCATACGAATCTTTAAATGAACTTTTGTTTCATATAAATTTTCGGGTTTTACCAAGTTTGGAAGGAACTTATTCAAATGCCTCTATTTGGGCGTTGACAGAGAGGATGTGTGGATTTTGACGCATTCTAAAATTCTTAACAATGGAAGTTTATTGTTGTTATTATGTATTTTGTAATTTTTGGCTCGTTTATTGGATTTTTCTTGTTTGTATAGCTTTTGCTCTGTAGTTTCCATCTTACAAAAGCTTATTTTTCAAATTAAAAAAGTGTTATTTCACTAAATCTATCCTACCATGAAGTCCACTAATCAATTGGCATTTGAAGCAAATATTATCTGTTAAAGCATTCTAGAATGATTTCATTCCAACTGTTGTTAGGATTTGCCCTGTTACCCATCTCAGCTGCCAAGTTGTTAGCTCTTTCACCATGTTTAACTTATTAATCATCCATAATTATTGTAATTTCATTGTTTATGTTCGTAATTTTTAAAAGTTGTTAATGTATGTGGGGTATGAAGTAACGACTATTGGTTGGCCTATGGTCATTGGGACAGGTAAAGAAGAATTGTTGTTCTTGAAGGGACCAAAGTACTCAATCTCCCAACTATTTTAATTTCCTTCTAAATTCAAAATGCCAGCCTCCGCTGAAATTGTTCTTAGAGTGACTCATGTCCATTGGAATTAGCAAGGGGTTGAAGTTTAGGAAACAGTAAATAGGTAGGAAGTATGGATGATGAGACCAAGGATCTTCTAAAAATCTAAATCAAAGAATCCTGACTACCTTTAAATTTCAGGAGGTGATCCACTTTTATATCAAGAAATGAAACCCTAAGCATATTTTGAACAATGGAGACATCATTCTTATAGCTTCCTTACATATCGTCTATGAATAACTTTGCACCAATACCATTTGTTCATTGGCGAAGCTTATCAGTGAGGACACCTCCCACCACGAGAGCTGAGTGCCTGAGCGCATGCCTAGGTGGAACTTTTAAAATTATGACCTTGCACTTTGGGCTCAGATGGCCATCATGCTTCAATGCACTTTAGGAAGATGCACAAGTCTTTTTGGTGAATAAACTTACTACTTTGAGTTTGTATCATCGTGCTAGTTATGTAGGACAAATGTTATTACATTATTAGAAAATTATAGTCATTAGCTAGGGATTTTATTAGAGAAGTAGTCATAAATAAAGTGTGGCTTGAGATAGGTGTATAATTGGGTATTATCTGAGGAGAGAACTAAGCCTCTCTAATGCTTGACAACCCATTGTAGTTCTAATCCATTCATTAGCCTTATCAATATCATATTTTCATTCATTTCCATTTCCTATTTTGTTTCCTATCAAATTGGTGTTAGAGCACCAACCACTAGGGAATATGCCCAACTATTTTCTAGTTATCTCCTAGGGGAACCCTTACTAATGGTTAGTGCTGCTGATTTTTGAGGGAACAGATTCTGATATTTGGGCTTTGATAAATTTATCCAGTCAACCAGACTGAGTTAGACAAGAGATCAAAGTTTTTGCATGTTTGATGTGGTATTAAGTCTGTACCATGGATTGATAGGACTTGTGCAAACTTGGACATTTATCAATTTTTCATTCTAGACTGATTTCTTCCCGTTCAAATTGAGCTTAATAATATGGAAAACTCATGATGTCTCCATCTTGGGGCTGGCTCAATTGCTTTTGAATAGAGAGTTGGATAGCCACTAGTTTCCTATTGAACCTTTAAAATATTGAAATATTAAAAGTTGACACCCACAAGGAGGCATAGTGACCTATAGAAAGATACTCTAATCCAACAAAATTATACTAAGATCATAATTACAAAAGACCTAGTAATTGACACCCACAAGGAGGCATTAAACTTCATCACCACCCAAACATTGTGACACGGTCTCTTTGTCTCTCCAAATATCCTATTATTCTTTCAAGCCAAACGCCCCACAAGATAGCAAAACCCAGCAAATTCTCAAGTCAGACCACTCAAAAGGCAAATCGAACTGATTCACTCCTTTTGACATACCAGAAAGAAGCCTGGTAGTACCCTGGTGTGTTAGTTAGTGGGCTAAATCTTTCAAACACTTTGTTATCCTTCGTCACTCTGCTCTGGCTTAGCATTTCCGATAGCATTTTTGTTAGCCTCCCTTTCAACTTTTGGTTCCTATCAAACTTTTGTTTTCTTTGATGATGAGAAACAAGTTAAATTAAGATAGAGAGGGAAAAGTATGGGAAAAAAAGGCATGACATTGTTGAAAGACTCCCCATTTCTTGCCAGCAAAATCTTCCACAAAGTGCTACTTTCATTTCTTTGCACGGCAGAACTTTAAACTTCAGATTTATAGAGAGATACAACATAACATTTCAATCTTGAACCTCAAATGTAAAGACAATTAGGACTATGACCCAGATCAATATTTTGCTCTTTCAGGATAAATTTAGCATTCTGGGATGAATTGGCACATGTTGCTTCTCAACACATTCAGAAAGGCAACCAGATATATGTATCTGGCCGGATGGTCGCAGATGTAGTTGAAGCTGATGATGGAAAACAGCAAACTTACCATAAGGTTTTCCCGTCCTTGTTCTCATTGTTCCATGGTTTTCTGTTGATTCATGAAGTAGATTCTGATGCTTTCATATCTTTTGCAGGTAGTAGTTCAGCAACTGAATTTTATAGAAAGAAGCAACTCAGCAGCCCCTCATTGTGATCAAGACTCTAATTCTGCAATGGAAGGCACGGGATACGGGATATTCTTTAATTTAAGATTATTGGTATGACACTTTGCTTCAGAATCTTATTATTTGATCCTATCAAATTTCTCCGCAGGGAGAAAGCTCGGCAGTGGTGCTGTTGATAATATGGGGGGGTCCACACAAGAACTATGGCAAGCATTCTTTGCCAATCCCGTTAACTGGTGGGACAATAGGAAAAACAAGGTACTTGATTAATTATTCTCTTGCAATTTTCAACATTCAAATGTCTTGTTTTTGGATTTAAATGTTCTTAACATGGACCAAGTCTGGTATTTTGCTTGTGCTTTTATGTATTTTATTACTTCTAATTGTATTTTGCCATATTGATGCATAAAAAAACCTTGAGAACTTACAATGAACTGCATGACATAATAACACATAGTTTTATGTATGTTATTATTTCTATTGCAGAGGGCAGGCCACTTGGATGCTTGCATTTTTTTAGACGAATAAGTTATTTAGGTTTTTTTTGTTAAAAAAAAAGAAACAACAATAATAAAAAGGAAAATGTTCCAGGGATACGAACTCCAAAGGGGAGTGGGAATAAAGAAAGAAAAAAAAAACAAAATCAAGAATTTATAGAAAAAGGAAAAATCAAAAACTTTGAGCCGAACACAAGCAAATAGTGGTTACCCTGCGAACAATATATTTATCTAATAAAACACTCCGCCTTCTTTGGACCAAAAACTTTTAGAGTTTGCAAATCAAGGCTGAAGTGAAGAAGGCTTCACAAACCACCCCTAAAGCAGCCGCAGCTAAAAACAAGGTTAAAACAAACACAGTCGACAGGAACACTGCCAAAAGAATCTTCAATCAGAGCTTTCTGAAAAATTGTTGGATGGATATTTACATACTTTACAGCTACAAATATTCAAATACCAGAAAACTAGGTGCAACCTCACAACTGTGCGTGACAAATAGCTTGAAAATCTATGCAAACAACGTGAGGGCAGCATTTTCAACAGCAAAGGAACCGAGTGTAATTGAGGCTGTAAAGATTTGCTAAAGGCACAAAACAAAAGCAACCAAGTTGAAAACAGAGCAGACTAAAAACAACTTATCATATGCCAAAAAGGCAAAAGCAACAACAAAGGAAGGAGCCAGCACAAGGCATAGAAAAAGCAAGCTTCCACAAGACCAAAGATCCCAAAAAAAAAAAAGCACAGCCACGAAGACGATGAAATCTCTAGGAGTCCATATGCTTGAAAACTTGAACTTCTTAATGTCTGTGATTTCATCTCGTAAACCTCAAGACCACAGACCCTTGGGAGGGAGGAAAAATTATCTCATAATGTGTTCACATGAATGGAGGAGTGGGACTTTGTATCAGAGAAATAATTTAAGAGGGAGGTTGATTTCTTCTAGAGAAATTTTGCATAGAACCCTATCAATATCTTGTTCAGATGAAATTTTATCATTCCCACTGTCAATTGGAAAATTGAAATCCATTTCCTCACTAGTAAGACTCACCATGGATTTGTTGTTTTATTCGAGCACATCCTCCAAACATCGACTTTTTGGTTGGTCCATTTGTTGAAAATATGTCATCCTTTGACAGACCTTTGTCAACTAAGAGGCTGAAATTCCAAAAGAGGGGAACTGGGAGAAGAGTTGAATCTTGAGCCAACAGTCATCAGCAGACTATTTTTTAGTTATCATATCAAAATATTATTATTTATTGTATATATGAAATGGTGGGCCTTAATGCTTGAAAAAGGAAAGAAAAAGGATTACTAGCCTCCCTTTGCAGGCCATTTTAGACATCCTAACCGTCATCTTTTGTTAGTATTTTTAGGTTCCCTTCACTATATGATTCACTTGAATCCTTAAGGAGAGATGAGATTCACTCTTTAATGTTAATACACCCGCATTTTGATTCTGCTTGCAGAAGAACCCAAAATATCCAGATTTTAAACACAAGGACACTGGGGAAGCCTTGTGGGTTGAGGGCAGGAATAATCCACCTTGGGTGAAGTCCCAGCTTGCACTATTGGACATGAGAATGGGTTCTCTTGATGATCAGGAAGCCAAAATGGCTAACTTTGTAAACAATTTTTGATTTCCCATCTTAATGAACCGACTCTGGATTCCTTTAAATTGTTTCCAACAGGGTATTAACCAAATATATATGGAAAGATTCTCGTGGTTCCTGGCTTGTATGGTTTGTAAATTGATGTGCTACATGTTAGATTGATAGTAATTCAAATTTTTCTTATGGGTCGGTACCTTTCATGATGGTATGTTGAAATATTTGTTTGTGTG

mRNA sequence

CGCGCACACAAAGGAACACAGTCGAAGATAAGAGGGAAATCGAGAGCGATGATAATGGCGGTAGAGAAAACCCTAACTCTTTCGTCGTCATTTTTATCAAAGAACTGCATCTTCTCCTTCTCTAGAAACAAGTCACGAGTCGCTTCCTTCTCCCATTTTCCCTCGCATCTGAACGCTGCTAAACACAGCCGTTTCGCTACTCGTTTCCTAAAATGCTTGGCTATGGAAAACTACAGCATTTACTCTGTGTCCTACCCCAAACCTTCCGAAATTCCATGGAAGAAGGAGCTCAGTAACTCGGTCAGTCTCATCGGGACAGTTGGTACGCCCGTGGAGCTCAAGCACCTACCATCGGGCAAAGATGTCGCGCGGACGCGACTTGCTGTGTGGAAGTCATCTACTGAAACATCTTGGATAAATTTAGCATTCTGGGATGAATTGGCACATGTTGCTTCTCAACACATTCAGAAAGGCAACCAGATATATGTATCTGGCCGGATGGTCGCAGATGTAGTTGAAGCTGATGATGGAAAACAGCAAACTTACCATAAGGTAGTAGTTCAGCAACTGAATTTTATAGAAAGAAGCAACTCAGCAGCCCCTCATTGTGATCAAGACTCTAATTCTGCAATGGAAGGGAGAAAGCTCGGCAGTGGTGCTGTTGATAATATGGGGGGGTCCACACAAGAACTATGGCAAGCATTCTTTGCCAATCCCGTTAACTGGTGGGACAATAGGAAAAACAAGAAGAACCCAAAATATCCAGATTTTAAACACAAGGACACTGGGGAAGCCTTGTGGGTTGAGGGCAGGAATAATCCACCTTGGGTGAAGTCCCAGCTTGCACTATTGGACATGAGAATGGGTTCTCTTGATGATCAGGAAGCCAAAATGGCTAACTTTGTAAACAATTTTTGATTTCCCATCTTAATGAACCGACTCTGGATTCCTTTAAATTGTTTCCAACAGGGTATTAACCAAATATATATGGAAAGATTCTCGTGGTTCCTGGCTTGTATGGTTTGTAAATTGATGTGCTACATGTTAGATTGATAGTAATTCAAATTTTTCTTATGGGTCGGTACCTTTCATGATGGTATGTTGAAATATTTGTTTGTGTG

Coding sequence (CDS)

ATGATAATGGCGGTAGAGAAAACCCTAACTCTTTCGTCGTCATTTTTATCAAAGAACTGCATCTTCTCCTTCTCTAGAAACAAGTCACGAGTCGCTTCCTTCTCCCATTTTCCCTCGCATCTGAACGCTGCTAAACACAGCCGTTTCGCTACTCGTTTCCTAAAATGCTTGGCTATGGAAAACTACAGCATTTACTCTGTGTCCTACCCCAAACCTTCCGAAATTCCATGGAAGAAGGAGCTCAGTAACTCGGTCAGTCTCATCGGGACAGTTGGTACGCCCGTGGAGCTCAAGCACCTACCATCGGGCAAAGATGTCGCGCGGACGCGACTTGCTGTGTGGAAGTCATCTACTGAAACATCTTGGATAAATTTAGCATTCTGGGATGAATTGGCACATGTTGCTTCTCAACACATTCAGAAAGGCAACCAGATATATGTATCTGGCCGGATGGTCGCAGATGTAGTTGAAGCTGATGATGGAAAACAGCAAACTTACCATAAGGTAGTAGTTCAGCAACTGAATTTTATAGAAAGAAGCAACTCAGCAGCCCCTCATTGTGATCAAGACTCTAATTCTGCAATGGAAGGGAGAAAGCTCGGCAGTGGTGCTGTTGATAATATGGGGGGGTCCACACAAGAACTATGGCAAGCATTCTTTGCCAATCCCGTTAACTGGTGGGACAATAGGAAAAACAAGAAGAACCCAAAATATCCAGATTTTAAACACAAGGACACTGGGGAAGCCTTGTGGGTTGAGGGCAGGAATAATCCACCTTGGGTGAAGTCCCAGCTTGCACTATTGGACATGAGAATGGGTTCTCTTGATGATCAGGAAGCCAAAATGGCTAACTTTGTAAACAATTTTTGA

Protein sequence

MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAMENYSIYSVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTETSWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERSNSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPDFKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF
Homology
BLAST of CmaCh17G012140 vs. ExPASy Swiss-Prot
Match: Q9SX99 (Protein OSB1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OSB1 PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.7e-24
Identity = 72/218 (33.03%), Postives = 111/218 (50.92%), Query Frame = 0

Query: 69  YPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWK--SSTETSWINLA 128
           + KP     K  L NSVSL+G V   +++ +    +    T L V    +   +  I+L 
Sbjct: 42  FKKPLSTKLKFNLVNSVSLMGFVDRSIQVMNTGPDRFGVFTILRVKDPLNPNRSFRISLR 101

Query: 129 FWDELAHVASQHIQKGNQIYVSGRMVADVVEADD---GKQQTYHKVVVQQLNFIERSNSA 188
            WD +A     H++  + I VSGR+ +    + D   G    Y +V V ++N++    +A
Sbjct: 102 MWDAMARTCIAHLKLNDHILVSGRLESYSKSSSDVYSGLNLDY-QVKVAEVNYV----AA 161

Query: 189 APHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPDFKH 248
            P    DS  +   +      ++        LWQ FF+NP +WWDNR+NKKNPK PDFKH
Sbjct: 162 PPSHVLDSQISKNPKTKTEDDIEESKKDEIYLWQVFFSNPYDWWDNRRNKKNPKQPDFKH 221

Query: 249 KDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAK 282
           KDTGEALW+   + P W+  +L L D +    D+++ +
Sbjct: 222 KDTGEALWL-CSDLPDWITRRLELFDQKNRFYDEEKTR 253

BLAST of CmaCh17G012140 vs. ExPASy Swiss-Prot
Match: Q8GXH3 (Protein OSB2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OSB2 PE=1 SV=2)

HSP 1 Score: 107.5 bits (267), Expect = 2.6e-22
Identity = 69/209 (33.01%), Postives = 111/209 (53.11%), Query Frame = 0

Query: 70  PKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTETS-WINLAFW 129
           P+P+EI ++ E++N V+LIG V  PV+ +    GK  A T ++   +S  +  WI + F 
Sbjct: 86  PRPNEIAYESEVANWVNLIGFVDQPVQFEASSDGKFWAGTVISQRSASDSSGFWIPIIFE 145

Query: 130 DELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIE-RSNSAAPHC 189
            +LA  A++++ K +QI+VSG++  D    +    Q   +V+VQ LNFI+  S S +P  
Sbjct: 146 GDLAKTAARYVSKDDQIHVSGKLFIDSPPPNMTYAQANVQVLVQNLNFIQPMSPSPSPFM 205

Query: 190 DQDSNSAMEG---RKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKN----PKYPD 249
              S+   E    ++      D +     + W     NP  WWD+R+NK N    P++PD
Sbjct: 206 VMSSSEKEESGIKKQPARAKQDIVIDEASDSWNHLIENPKEWWDHRENKVNGLVKPRHPD 265

Query: 250 FKHKDTGEALWVEGRNNPPWVKSQLALLD 270
           FK KD+  +LW+     P WV  +L  L+
Sbjct: 266 FKSKDSSFSLWL--NKAPNWVLPKLEGLE 292

BLAST of CmaCh17G012140 vs. ExPASy Swiss-Prot
Match: Q9FYJ2 (Protein OSB4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OSB4 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 5.0e-21
Identity = 70/233 (30.04%), Postives = 117/233 (50.21%), Query Frame = 0

Query: 66  SVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTETS---- 125
           S  +P+P E+P++ +++NS+ LIG V  PV+      GK  A T ++   SS   S    
Sbjct: 56  SEEWPRPMEVPYQPKIANSIDLIGYVHQPVQFDSTLDGKFWAGTVISHEPSSDSKSESDS 115

Query: 126 ----WINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTY----------- 185
               WI + F  +LAH A+ +++K ++++++G+++ DV+++     Q +           
Sbjct: 116 SSNFWIPVLFEGDLAHTANSYLKKNDRVHITGQILGDVIQSGANSDQAHVQLFKSFHGSF 175

Query: 186 -HKVVVQQLNFIERSNS---AAPHCDQD------SNSAMEGRKLGSGAVDNMGGSTQELW 245
            H+V+V+ L++IE S +     P  DQ+      S S   GR+ G+            LW
Sbjct: 176 SHQVMVRDLHYIEGSKAMPKVLPTLDQNEGVLKHSASVQRGREFGT-----------NLW 235

Query: 246 QAFFANPVNWWDNRKNKK----NPKYPDFKHKDTGEALWVEGRNNPPWVKSQL 266
                 P  W D R+ K+    NPK+PDFK KD  +ALW+   N P  + S+L
Sbjct: 236 FDLVDKPNEWCDYREMKQNGSVNPKHPDFKKKDGSQALWL--NNAPTEILSEL 275

BLAST of CmaCh17G012140 vs. ExPASy Swiss-Prot
Match: Q8GWJ4 (Protein OSB3, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OSB3 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.0e-18
Identity = 63/201 (31.34%), Postives = 103/201 (51.24%), Query Frame = 0

Query: 72  PSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTETS-WINLAFWDE 131
           P +I +K E+SN ++LIG V  PV+      GK  A T ++    S  ++ WI + F  +
Sbjct: 71  PKKIEYKPEISNWINLIGFVEQPVQFGPCSDGKFWAGTVISQRSGSKSSNFWIPIIFEGD 130

Query: 132 LAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERSNSAAPHCD-- 191
           LA +A QH++K ++I+VSG++  D    +    Q+  +V+VQ LNF++ + S        
Sbjct: 131 LAKIAVQHVKKEDRIHVSGKLFIDSPPPNVTYSQSNVQVMVQNLNFVQAATSTTKTISPP 190

Query: 192 QDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKN----PKYPDFKHK 251
           +   ++++ +   S  V  +   T   W+    NP  W D+R NK N    P +PDFK K
Sbjct: 191 EKEVTSIKKKPARSKKVKVIDEETSNSWKHLIENPKEWLDHRGNKANGLVKPGHPDFKMK 250

Query: 252 DTGEALWVEGRNNPPWVKSQL 266
             G +LW+     P W   +L
Sbjct: 251 VGGLSLWLS--TAPDWALLKL 269

BLAST of CmaCh17G012140 vs. ExPASy Swiss-Prot
Match: Q8Y2B4 (Single-stranded DNA-binding protein OS=Ralstonia solanacearum (strain GMI1000) OX=267608 GN=ssb PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.7e-08
Identity = 34/104 (32.69%), Postives = 59/104 (56.73%), Query Frame = 0

Query: 83  NSVSLIGTVGTPVELKHLPSGKDVARTRLAVW-----KSSTE----TSWINLAFWDELAH 142
           N V ++G +G   E +++PSG  V   R+A       K+S E    T W  +AF+  LA 
Sbjct: 5   NKVIIVGNLGADPETRYMPSGDAVTNIRVATTDRYKDKASGEMREATEWHRIAFFGRLAE 64

Query: 143 VASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFI 178
           +A ++++KG+Q+YV GR+     E  DG++Q   ++V +Q+  +
Sbjct: 65  IAGEYLKKGSQVYVEGRLKTRQWE-KDGQKQYSTEIVAEQMQML 107

BLAST of CmaCh17G012140 vs. ExPASy TrEMBL
Match: A0A6J1JVR7 (protein OSB2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111487976 PE=4 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 1.9e-164
Identity = 289/289 (100.00%), Postives = 289/289 (100.00%), Query Frame = 0

Query: 1   MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME 60
           MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME
Sbjct: 1   MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME 60

Query: 61  NYSIYSVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTET 120
           NYSIYSVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTET
Sbjct: 61  NYSIYSVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTET 120

Query: 121 SWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERS 180
           SWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERS
Sbjct: 121 SWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERS 180

Query: 181 NSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240
           NSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD
Sbjct: 181 NSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240

Query: 241 FKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF 290
           FKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF
Sbjct: 241 FKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF 289

BLAST of CmaCh17G012140 vs. ExPASy TrEMBL
Match: A0A6J1GQ31 (protein OSB2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456464 PE=4 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 8.8e-154
Identity = 271/289 (93.77%), Postives = 279/289 (96.54%), Query Frame = 0

Query: 1   MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME 60
           MIMAV+KTLTLSSSFLSK+CIFSFSRNKSR+ASFS F SHLNAAKHSRFATRF KCLAME
Sbjct: 1   MIMAVQKTLTLSSSFLSKSCIFSFSRNKSRIASFSPFLSHLNAAKHSRFATRFPKCLAME 60

Query: 61  NYSIYSVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTET 120
           NYS YSVSYPKPSEIPWKKELSNSVSLIG VGTPVELK LP+GKDVARTRLAVWKSSTET
Sbjct: 61  NYSTYSVSYPKPSEIPWKKELSNSVSLIGIVGTPVELKRLPTGKDVARTRLAVWKSSTET 120

Query: 121 SWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERS 180
           SWINLAFWDELAHVASQH+QKGNQIYVSGRMVADV EADDGKQQTYHKVVVQQLNFIERS
Sbjct: 121 SWINLAFWDELAHVASQHVQKGNQIYVSGRMVADVAEADDGKQQTYHKVVVQQLNFIERS 180

Query: 181 NSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240
           NS APHCDQDSNSAMEG+KLGSGAVDN+GGSTQELWQAFFANPVNWWDNRKNKKNPKYPD
Sbjct: 181 NSTAPHCDQDSNSAMEGKKLGSGAVDNVGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240

Query: 241 FKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF 290
           FKHKDTGEALWVEG NNPPWVKSQLALLDMRMGSLD+QEA MANFVNNF
Sbjct: 241 FKHKDTGEALWVEGWNNPPWVKSQLALLDMRMGSLDNQEANMANFVNNF 289

BLAST of CmaCh17G012140 vs. ExPASy TrEMBL
Match: A0A1S3BRV3 (protein OSB2, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492617 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 7.2e-132
Identity = 240/290 (82.76%), Postives = 258/290 (88.97%), Query Frame = 0

Query: 1   MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME 60
           M +A+E+TLTLSSSF SKN IFS  RN+ R++SF  F S+LN  KH RFATRF KC AME
Sbjct: 3   MALALEQTLTLSSSFSSKNSIFSIPRNQLRISSFFPFHSYLNPPKHLRFATRFPKCSAME 62

Query: 61  NYSIYS-VSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTE 120
           NY+ YS V+YPKPSEIPWKKELSNSV+LIGTVG PVELKHLPSGKDVA TRLAVWKSSTE
Sbjct: 63  NYTNYSAVTYPKPSEIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 122

Query: 121 TSWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIER 180
           TSWI+L FWDELAH+ASQH+QKGNQIYVSGR+VAD+VEADDGKQQTY+KVVVQQLNFIER
Sbjct: 123 TSWISLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLNFIER 182

Query: 181 SNSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYP 240
           SNS APH DQDSNS M GRK  SGAVDN   STQELWQAFFANPV+WWDNRKNKKNPKYP
Sbjct: 183 SNSTAPHYDQDSNSIMAGRKPSSGAVDNT-ASTQELWQAFFANPVDWWDNRKNKKNPKYP 242

Query: 241 DFKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF 290
           DFKHKDTGEALWVEGR NPPWVKSQLALLDMRM SLDDQE KM NFV+NF
Sbjct: 243 DFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEVKMTNFVSNF 291

BLAST of CmaCh17G012140 vs. ExPASy TrEMBL
Match: A0A5D3DR47 (Protein OSB2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00530 PE=4 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 2.8e-131
Identity = 239/290 (82.41%), Postives = 258/290 (88.97%), Query Frame = 0

Query: 1   MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME 60
           M +A+E+TLTLSSSF SKN IFS  RN+ R++SF  F S+LN  KH RFATRF KC AME
Sbjct: 3   MALALEQTLTLSSSFSSKNSIFSIPRNQLRISSFFPFHSYLNPPKHLRFATRFPKCSAME 62

Query: 61  NYSIYS-VSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTE 120
           NY+ YS V+YPKPSEIPWKKELSNSV+LIGTVG PVELKHLPSGKDVA TRLAVWKSSTE
Sbjct: 63  NYTNYSAVTYPKPSEIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 122

Query: 121 TSWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIER 180
           TSWI+L FWDELAH+ASQH+QKGNQIYVSGR+VAD+VEADDGKQQTY+KVVVQQLNFIER
Sbjct: 123 TSWISLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLNFIER 182

Query: 181 SNSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYP 240
           SNS APH DQDSNS M GRK  SGAVDN   STQELWQAFFANPV+WWDNRKNKK+PKYP
Sbjct: 183 SNSTAPHYDQDSNSIMAGRKPSSGAVDNT-ASTQELWQAFFANPVDWWDNRKNKKSPKYP 242

Query: 241 DFKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF 290
           DFKHKDTGEALWVEGR NPPWVKSQLALLDMRM SLDDQE KM NFV+NF
Sbjct: 243 DFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEVKMTNFVSNF 291

BLAST of CmaCh17G012140 vs. ExPASy TrEMBL
Match: A0A0A0K4W2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G378540 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.3e-130
Identity = 238/290 (82.07%), Postives = 256/290 (88.28%), Query Frame = 0

Query: 1   MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME 60
           M+MA+E+TL LSSS  SKN IFS  RN+  ++SF  F S+LN  KH RF   F KC AME
Sbjct: 1   MVMALEQTLILSSSLSSKNSIFSIPRNQLPISSFFPFHSYLNPPKHLRFPAPFPKCSAME 60

Query: 61  NYSIYS-VSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTE 120
           NY+ YS V+YPKPS+IPWKKELSNSV+LIGTVG PVELKHLPSGKDVA TRLAVWKSSTE
Sbjct: 61  NYTNYSAVTYPKPSQIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 120

Query: 121 TSWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIER 180
           TSWI L FWDELAH+ASQH+QKGNQIYVSGR+VAD+VEADDGKQQTY+KVVVQQLNFIER
Sbjct: 121 TSWIGLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLNFIER 180

Query: 181 SNSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYP 240
           SNS APH DQDSNS M GRK GSGAVDN  GSTQELWQAFFANPV+WWDNRKNKKNPKYP
Sbjct: 181 SNSTAPHYDQDSNSIMAGRKPGSGAVDNT-GSTQELWQAFFANPVDWWDNRKNKKNPKYP 240

Query: 241 DFKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF 290
           DFKHKDTGEALWVEGR NPPWVKSQLALLDMRMGSLDDQEAKMANFV +F
Sbjct: 241 DFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMGSLDDQEAKMANFVTSF 289

BLAST of CmaCh17G012140 vs. NCBI nr
Match: XP_022991273.1 (protein OSB2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 588.2 bits (1515), Expect = 3.9e-164
Identity = 289/289 (100.00%), Postives = 289/289 (100.00%), Query Frame = 0

Query: 1   MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME 60
           MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME
Sbjct: 1   MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME 60

Query: 61  NYSIYSVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTET 120
           NYSIYSVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTET
Sbjct: 61  NYSIYSVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTET 120

Query: 121 SWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERS 180
           SWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERS
Sbjct: 121 SWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERS 180

Query: 181 NSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240
           NSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD
Sbjct: 181 NSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240

Query: 241 FKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF 290
           FKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF
Sbjct: 241 FKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF 289

BLAST of CmaCh17G012140 vs. NCBI nr
Match: XP_022954097.1 (protein OSB2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 552.7 bits (1423), Expect = 1.8e-153
Identity = 271/289 (93.77%), Postives = 279/289 (96.54%), Query Frame = 0

Query: 1   MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME 60
           MIMAV+KTLTLSSSFLSK+CIFSFSRNKSR+ASFS F SHLNAAKHSRFATRF KCLAME
Sbjct: 1   MIMAVQKTLTLSSSFLSKSCIFSFSRNKSRIASFSPFLSHLNAAKHSRFATRFPKCLAME 60

Query: 61  NYSIYSVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTET 120
           NYS YSVSYPKPSEIPWKKELSNSVSLIG VGTPVELK LP+GKDVARTRLAVWKSSTET
Sbjct: 61  NYSTYSVSYPKPSEIPWKKELSNSVSLIGIVGTPVELKRLPTGKDVARTRLAVWKSSTET 120

Query: 121 SWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERS 180
           SWINLAFWDELAHVASQH+QKGNQIYVSGRMVADV EADDGKQQTYHKVVVQQLNFIERS
Sbjct: 121 SWINLAFWDELAHVASQHVQKGNQIYVSGRMVADVAEADDGKQQTYHKVVVQQLNFIERS 180

Query: 181 NSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240
           NS APHCDQDSNSAMEG+KLGSGAVDN+GGSTQELWQAFFANPVNWWDNRKNKKNPKYPD
Sbjct: 181 NSTAPHCDQDSNSAMEGKKLGSGAVDNVGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240

Query: 241 FKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF 290
           FKHKDTGEALWVEG NNPPWVKSQLALLDMRMGSLD+QEA MANFVNNF
Sbjct: 241 FKHKDTGEALWVEGWNNPPWVKSQLALLDMRMGSLDNQEANMANFVNNF 289

BLAST of CmaCh17G012140 vs. NCBI nr
Match: KAG6575942.1 (Protein OSB1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 548.1 bits (1411), Expect = 4.5e-152
Identity = 269/289 (93.08%), Postives = 279/289 (96.54%), Query Frame = 0

Query: 1   MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME 60
           MIMAVEK LTLSSSFLSK+CIFSFSRNKSR+ASFS F SHLNAAKHSRFATRF KCLAME
Sbjct: 1   MIMAVEKALTLSSSFLSKSCIFSFSRNKSRIASFSPFLSHLNAAKHSRFATRFPKCLAME 60

Query: 61  NYSIYSVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTET 120
           NYS YSVSYPKPSEIPWKKELSNSVSLIGTVG PVELKHLPSGKDVA TRLAVWKSSTET
Sbjct: 61  NYSTYSVSYPKPSEIPWKKELSNSVSLIGTVGAPVELKHLPSGKDVAWTRLAVWKSSTET 120

Query: 121 SWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERS 180
           SWINLAFWDELAHVASQH+QKGNQIYVSG+MVA+VVEADDGKQQTY+KVVVQQLNFIERS
Sbjct: 121 SWINLAFWDELAHVASQHVQKGNQIYVSGQMVANVVEADDGKQQTYYKVVVQQLNFIERS 180

Query: 181 NSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240
           NS APHCDQDSN+AMEG+KLGSGAVDN+GGSTQELWQAFFANPVNWWDNRKNKKNPKYPD
Sbjct: 181 NSTAPHCDQDSNTAMEGKKLGSGAVDNVGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240

Query: 241 FKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF 290
           FKHKDTGEALWVEG NNPPWVKSQLALLDMRMGSLD+QEA MANFVNNF
Sbjct: 241 FKHKDTGEALWVEGWNNPPWVKSQLALLDMRMGSLDNQEANMANFVNNF 289

BLAST of CmaCh17G012140 vs. NCBI nr
Match: XP_023549001.1 (protein OSB2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 543.9 bits (1400), Expect = 8.4e-151
Identity = 267/289 (92.39%), Postives = 277/289 (95.85%), Query Frame = 0

Query: 1   MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME 60
           MIMAVEKTL LSSSFLSKNCIFSFSRNK R+ASFS   SHLNAAKHSRFATRF KCLAME
Sbjct: 1   MIMAVEKTLILSSSFLSKNCIFSFSRNKLRIASFSPLLSHLNAAKHSRFATRFPKCLAME 60

Query: 61  NYSIYSVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTET 120
           NYS YSVSYPKPSEIPWKKELSNSVSLIGTVG PVELKHLPSGKDVA TRLAVWKSSTET
Sbjct: 61  NYSTYSVSYPKPSEIPWKKELSNSVSLIGTVGAPVELKHLPSGKDVAWTRLAVWKSSTET 120

Query: 121 SWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERS 180
           SWINLAFWDELAHVASQH+QKGNQIYVSG+MVA+V+EADDGKQQTY+KVVVQQLNFIERS
Sbjct: 121 SWINLAFWDELAHVASQHVQKGNQIYVSGQMVANVIEADDGKQQTYYKVVVQQLNFIERS 180

Query: 181 NSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240
           NS APHCDQDSN+AMEG+KL SGAVDN+GGSTQELWQAFFANPVNWWDNRKNKKNPKYPD
Sbjct: 181 NSTAPHCDQDSNTAMEGKKLDSGAVDNVGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240

Query: 241 FKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF 290
           FKHKDTGEALWVEG NNPPWVKSQLALLDMRMGSLD+QEAKMANFVNNF
Sbjct: 241 FKHKDTGEALWVEGWNNPPWVKSQLALLDMRMGSLDNQEAKMANFVNNF 289

BLAST of CmaCh17G012140 vs. NCBI nr
Match: KAG7014475.1 (Protein OSB1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 518.1 bits (1333), Expect = 5.0e-143
Identity = 259/289 (89.62%), Postives = 268/289 (92.73%), Query Frame = 0

Query: 1   MIMAVEKTLTLSSSFLSKNCIFSFSRNKSRVASFSHFPSHLNAAKHSRFATRFLKCLAME 60
           MIMAVEK LTLSSSFLSK+CIFSFSRNKSR+ASFS F SHLNAAKHSRFATRF KCLAME
Sbjct: 1   MIMAVEKALTLSSSFLSKSCIFSFSRNKSRIASFSPFLSHLNAAKHSRFATRFPKCLAME 60

Query: 61  NYSIYSVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTET 120
           NYS YSVSYPKPSEIPWKKELSNSVSLIGTVG PVELKHLPSGKDVA TRLAVWKSSTET
Sbjct: 61  NYSTYSVSYPKPSEIPWKKELSNSVSLIGTVGAPVELKHLPSGKDVAWTRLAVWKSSTET 120

Query: 121 SWINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERS 180
           SWINLAFWDELAHVASQH+QKGNQIYVSG+MVA+VVEADDGKQQTY+KVVVQQLNFIERS
Sbjct: 121 SWINLAFWDELAHVASQHVQKGNQIYVSGQMVANVVEADDGKQQTYYKVVVQQLNFIERS 180

Query: 181 NSAAPHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240
           NS APH           +KLGSGAVDN+GGSTQELWQAFFANPVNWWDNRKNKKNPKYPD
Sbjct: 181 NSTAPH----------WKKLGSGAVDNVGGSTQELWQAFFANPVNWWDNRKNKKNPKYPD 240

Query: 241 FKHKDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAKMANFVNNF 290
           FKHKDTGEALWVEG NNPPWVKSQLALLDMRMGSLD+QEA MANFVNNF
Sbjct: 241 FKHKDTGEALWVEGWNNPPWVKSQLALLDMRMGSLDNQEANMANFVNNF 279

BLAST of CmaCh17G012140 vs. TAIR 10
Match: AT1G47720.1 (Primosome PriB/single-strand DNA-binding )

HSP 1 Score: 114.8 bits (286), Expect = 1.2e-25
Identity = 72/218 (33.03%), Postives = 111/218 (50.92%), Query Frame = 0

Query: 69  YPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWK--SSTETSWINLA 128
           + KP     K  L NSVSL+G V   +++ +    +    T L V    +   +  I+L 
Sbjct: 42  FKKPLSTKLKFNLVNSVSLMGFVDRSIQVMNTGPDRFGVFTILRVKDPLNPNRSFRISLR 101

Query: 129 FWDELAHVASQHIQKGNQIYVSGRMVADVVEADD---GKQQTYHKVVVQQLNFIERSNSA 188
            WD +A     H++  + I VSGR+ +    + D   G    Y +V V ++N++    +A
Sbjct: 102 MWDAMARTCIAHLKLNDHILVSGRLESYSKSSSDVYSGLNLDY-QVKVAEVNYV----AA 161

Query: 189 APHCDQDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKNPKYPDFKH 248
            P    DS  +   +      ++        LWQ FF+NP +WWDNR+NKKNPK PDFKH
Sbjct: 162 PPSHVLDSQISKNPKTKTEDDIEESKKDEIYLWQVFFSNPYDWWDNRRNKKNPKQPDFKH 221

Query: 249 KDTGEALWVEGRNNPPWVKSQLALLDMRMGSLDDQEAK 282
           KDTGEALW+   + P W+  +L L D +    D+++ +
Sbjct: 222 KDTGEALWL-CSDLPDWITRRLELFDQKNRFYDEEKTR 253

BLAST of CmaCh17G012140 vs. TAIR 10
Match: AT4G20010.2 (plastid transcriptionally active 9 )

HSP 1 Score: 109.4 bits (272), Expect = 4.9e-24
Identity = 70/209 (33.49%), Postives = 112/209 (53.59%), Query Frame = 0

Query: 70  PKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTETS-WINLAFW 129
           P+P+EI ++ E++N V+LIG V  PV+ +    GK  A T ++   +S  +  WI + F 
Sbjct: 86  PRPNEIAYESEVANWVNLIGFVDQPVQFEASSDGKFWAGTVISQRSASDSSGFWIPIIFE 145

Query: 130 DELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIE-RSNSAAPHC 189
            +LA  A++++ K +QI+VSG++  D    +    Q   +V+VQ LNFI+  S S +P  
Sbjct: 146 GDLAKTAARYVSKDDQIHVSGKLFIDSPPPNMTYAQANVQVLVQNLNFIQPMSPSPSPFM 205

Query: 190 DQDSNSAMEG---RKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKN----PKYPD 249
              S+   E    ++ G    D +     + W     NP  WWD+R+NK N    P++PD
Sbjct: 206 VMSSSEKEESGIKKQPGIAKQDIVIDEASDSWNHLIENPKEWWDHRENKVNGLVKPRHPD 265

Query: 250 FKHKDTGEALWVEGRNNPPWVKSQLALLD 270
           FK KD+  +LW+     P WV  +L  L+
Sbjct: 266 FKSKDSSFSLWL--NKAPNWVLPKLEGLE 292

BLAST of CmaCh17G012140 vs. TAIR 10
Match: AT4G20010.1 (plastid transcriptionally active 9 )

HSP 1 Score: 107.5 bits (267), Expect = 1.9e-23
Identity = 69/209 (33.01%), Postives = 111/209 (53.11%), Query Frame = 0

Query: 70  PKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTETS-WINLAFW 129
           P+P+EI ++ E++N V+LIG V  PV+ +    GK  A T ++   +S  +  WI + F 
Sbjct: 86  PRPNEIAYESEVANWVNLIGFVDQPVQFEASSDGKFWAGTVISQRSASDSSGFWIPIIFE 145

Query: 130 DELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIE-RSNSAAPHC 189
            +LA  A++++ K +QI+VSG++  D    +    Q   +V+VQ LNFI+  S S +P  
Sbjct: 146 GDLAKTAARYVSKDDQIHVSGKLFIDSPPPNMTYAQANVQVLVQNLNFIQPMSPSPSPFM 205

Query: 190 DQDSNSAMEG---RKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKN----PKYPD 249
              S+   E    ++      D +     + W     NP  WWD+R+NK N    P++PD
Sbjct: 206 VMSSSEKEESGIKKQPARAKQDIVIDEASDSWNHLIENPKEWWDHRENKVNGLVKPRHPD 265

Query: 250 FKHKDTGEALWVEGRNNPPWVKSQLALLD 270
           FK KD+  +LW+     P WV  +L  L+
Sbjct: 266 FKSKDSSFSLWL--NKAPNWVLPKLEGLE 292

BLAST of CmaCh17G012140 vs. TAIR 10
Match: AT1G31010.1 (organellar single-stranded DNA binding protein 4 )

HSP 1 Score: 103.2 bits (256), Expect = 3.5e-22
Identity = 70/233 (30.04%), Postives = 117/233 (50.21%), Query Frame = 0

Query: 66  SVSYPKPSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTETS---- 125
           S  +P+P E+P++ +++NS+ LIG V  PV+      GK  A T ++   SS   S    
Sbjct: 56  SEEWPRPMEVPYQPKIANSIDLIGYVHQPVQFDSTLDGKFWAGTVISHEPSSDSKSESDS 115

Query: 126 ----WINLAFWDELAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTY----------- 185
               WI + F  +LAH A+ +++K ++++++G+++ DV+++     Q +           
Sbjct: 116 SSNFWIPVLFEGDLAHTANSYLKKNDRVHITGQILGDVIQSGANSDQAHVQLFKSFHGSF 175

Query: 186 -HKVVVQQLNFIERSNS---AAPHCDQD------SNSAMEGRKLGSGAVDNMGGSTQELW 245
            H+V+V+ L++IE S +     P  DQ+      S S   GR+ G+            LW
Sbjct: 176 SHQVMVRDLHYIEGSKAMPKVLPTLDQNEGVLKHSASVQRGREFGT-----------NLW 235

Query: 246 QAFFANPVNWWDNRKNKK----NPKYPDFKHKDTGEALWVEGRNNPPWVKSQL 266
                 P  W D R+ K+    NPK+PDFK KD  +ALW+   N P  + S+L
Sbjct: 236 FDLVDKPNEWCDYREMKQNGSVNPKHPDFKKKDGSQALWL--NNAPTEILSEL 275

BLAST of CmaCh17G012140 vs. TAIR 10
Match: AT5G44785.2 (organellar single-stranded DNA binding protein 3 )

HSP 1 Score: 95.5 bits (236), Expect = 7.4e-20
Identity = 63/201 (31.34%), Postives = 103/201 (51.24%), Query Frame = 0

Query: 72  PSEIPWKKELSNSVSLIGTVGTPVELKHLPSGKDVARTRLAVWKSSTETS-WINLAFWDE 131
           P +I +K E+SN ++LIG V  PV+      GK  A T ++    S  ++ WI + F  +
Sbjct: 71  PKKIEYKPEISNWINLIGFVEQPVQFGPCSDGKFWAGTVISQRSGSKSSNFWIPIIFEGD 130

Query: 132 LAHVASQHIQKGNQIYVSGRMVADVVEADDGKQQTYHKVVVQQLNFIERSNSAAPHCD-- 191
           LA +A QH++K ++I+VSG++  D    +    Q+  +V+VQ LNF++ + S        
Sbjct: 131 LAKIAVQHVKKEDRIHVSGKLFIDSPPPNVTYSQSNVQVMVQNLNFVQAATSTTKTISPP 190

Query: 192 QDSNSAMEGRKLGSGAVDNMGGSTQELWQAFFANPVNWWDNRKNKKN----PKYPDFKHK 251
           +   ++++ +   S  V  +   T   W+    NP  W D+R NK N    P +PDFK K
Sbjct: 191 EKEVTSIKKKPARSKKVKVIDEETSNSWKHLIENPKEWLDHRGNKANGLVKPGHPDFKMK 250

Query: 252 DTGEALWVEGRNNPPWVKSQL 266
             G +LW+     P W   +L
Sbjct: 251 VGGLSLWLS--TAPDWALLKL 269

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SX991.7e-2433.03Protein OSB1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OSB1 PE=1 SV=1[more]
Q8GXH32.6e-2233.01Protein OSB2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OSB2 PE=1 SV=2[more]
Q9FYJ25.0e-2130.04Protein OSB4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OSB4 PE=2 SV=1[more]
Q8GWJ41.0e-1831.34Protein OSB3, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OSB... [more]
Q8Y2B41.7e-0832.69Single-stranded DNA-binding protein OS=Ralstonia solanacearum (strain GMI1000) O... [more]
Match NameE-valueIdentityDescription
A0A6J1JVR71.9e-164100.00protein OSB2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111487976 PE=... [more]
A0A6J1GQ318.8e-15493.77protein OSB2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456464 P... [more]
A0A1S3BRV37.2e-13282.76protein OSB2, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492617 PE=4 SV... [more]
A0A5D3DR472.8e-13182.41Protein OSB2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00530 P... [more]
A0A0A0K4W22.3e-13082.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G378540 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022991273.13.9e-164100.00protein OSB2, chloroplastic-like [Cucurbita maxima][more]
XP_022954097.11.8e-15393.77protein OSB2, chloroplastic-like [Cucurbita moschata][more]
KAG6575942.14.5e-15293.08Protein OSB1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023549001.18.4e-15192.39protein OSB2, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG7014475.15.0e-14389.62Protein OSB1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT1G47720.11.2e-2533.03Primosome PriB/single-strand DNA-binding [more]
AT4G20010.24.9e-2433.49plastid transcriptionally active 9 [more]
AT4G20010.11.9e-2333.01plastid transcriptionally active 9 [more]
AT1G31010.13.5e-2230.04organellar single-stranded DNA binding protein 4 [more]
AT5G44785.27.4e-2031.34organellar single-stranded DNA binding protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000424Primosome PriB/single-strand DNA-bindingPFAMPF00436SSBcoord: 83..176
e-value: 4.8E-19
score: 68.4
IPR000424Primosome PriB/single-strand DNA-bindingPROSITEPS50935SSBcoord: 81..178
score: 22.686615
IPR000424Primosome PriB/single-strand DNA-bindingCDDcd04496SSB_OBFcoord: 85..177
e-value: 4.09638E-22
score: 86.5121
IPR011344Single-stranded DNA-binding proteinTIGRFAMTIGR00621TIGR00621coord: 83..193
e-value: 1.9E-18
score: 65.3
IPR011344Single-stranded DNA-binding proteinPANTHERPTHR10302SINGLE-STRANDED DNA-BINDING PROTEINcoord: 23..284
NoneNo IPR availableGENE3D2.40.50.140coord: 79..186
e-value: 5.2E-23
score: 83.3
NoneNo IPR availablePANTHERPTHR10302:SF15OS03G0633900 PROTEINcoord: 23..284
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 83..263

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G012140.1CmaCh17G012140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006264 mitochondrial DNA replication
biological_process GO:0051096 positive regulation of helicase activity
biological_process GO:0006260 DNA replication
cellular_component GO:0042645 mitochondrial nucleoid
molecular_function GO:0003697 single-stranded DNA binding