CmaCh17G011650 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G011650
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionadenylate isopentenyltransferase 3, chloroplastic-like
LocationCma_Chr17: 8090132 .. 8091124 (-)
RNA-Seq ExpressionCmaCh17G011650
SyntenyCmaCh17G011650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGTGTCCACCATGTTCATGAGCAAAGAGATGATCACTCAACCATTGATAAATCTTCCTCCTCGAAGAATAAACTCCAACCCTTTCTTCAATCTCCCAAAGCTCGGAAAAGAGAAGGTGGTGATCGTGATCGGCGCTACTGGCACGGGTAAGTCTCGGTTGTCGATCGACATTGCTACCCGCTTCTCGATGGAAGTTATAAACTCTGATAAAATGCAAGTTTACGATGGGCTAGATATAGTTACAAATAAGGTCACGAAGAGAGAACAACGTGGTGTACCGCATCACTTGCTTGGAGTTATTGATCCCTTTTGTGACTTTTCGGCAGCTGACTTCTCTGATATGACTATGTTATCTTTGGATTCTATTTATAGCCGTGGTCGGCTCCCGATTATCGCTGGTGGCTCGAATTCTTATATTGAAACCTTAGTTGACTCGAACTTTGAATCGAGATATGAATGTTGCTTTATTTGGGTAGATGTGTCAATACCTGTATTGCAATCCTTTGTATCTAATAGGGTAGACAAAATGGTAGCTAAGGGAATGGTTGACGAGGCCGAAAAATTCTTTGATCCCACTCGAGATTATTCTCGAGGGATCAAAAGGGCAATTGGGGTTCCCGAATTCGACTCCTATTTTCGATACAAATCGTTCTTAAACGAGGAAACTCAAGAGAAACTCTTACAAGAATCCATATCAAAAGTGAAGGAGCACACATGCAAGCTAGCATTGAGGCAATTGGGAAAGATCCAACGACTGAGAAACACCAAAGAATGGAACATACACAGATTGGATGCTACAGAGGCCTTTACAAAGCGAGGCAAAGAAGCAGAAGAAGCATGGAACAAGCTCGTCACGACACGAAGCACAACGATAATCGACCAATTTCTTCATCCGAAGATCTTCACCGTCAAAACCCAGAAGAATTTAGCATCCCTCCCCGGCGTGCCGCCTAAGAAAAGTGCAGCCATTGCAGCTGCAACACCTTGA

mRNA sequence

ATGAATGTGTCCACCATGTTCATGAGCAAAGAGATGATCACTCAACCATTGATAAATCTTCCTCCTCGAAGAATAAACTCCAACCCTTTCTTCAATCTCCCAAAGCTCGGAAAAGAGAAGGTGGTGATCGTGATCGGCGCTACTGGCACGGGTAAGTCTCGGTTGTCGATCGACATTGCTACCCGCTTCTCGATGGAAGTTATAAACTCTGATAAAATGCAAGTTTACGATGGGCTAGATATAGTTACAAATAAGGTCACGAAGAGAGAACAACGTGGTGTACCGCATCACTTGCTTGGAGTTATTGATCCCTTTTGTGACTTTTCGGCAGCTGACTTCTCTGATATGACTATCCGTGGTCGGCTCCCGATTATCGCTGGTGGCTCGAATTCTTATATTGAAACCTTAGTTGACTCGAACTTTGAATCGAGATATGAATGTTGCTTTATTTGGGTAGATGTGTCAATACCTGTATTGCAATCCTTTGTATCTAATAGGGTAGACAAAATGGTAGCTAAGGGAATGGTTGACGAGGCCGAAAAATTCTTTGATCCCACTCGAGATTATTCTCGAGGGATCAAAAGGGCAATTGGGGTTCCCGAATTCGACTCCTATTTTCGATACAAATCGTTCTTAAACGAGGAAACTCAAGAGAAACTCTTACAAGAATCCATATCAAAAGTGAAGGAGCACACATGCAAGCTAGCATTGAGGCAATTGGGAAAGATCCAACGACTGAGAAACACCAAAGAATGGAACATACACAGATTGGATGCTACAGAGGCCTTTACAAAGCGAGGCAAAGAAGCAGAAGAAGCATGGAACAAGCTCGTCACGACACGAAGCACAACGATAATCGACCAATTTCTTCATCCGAAGATCTTCACCGTCAAAACCCAGAAGAATTTAGCATCCCTCCCCGGCGTGCCGCCTAAGAAAAGTGCAGCCATTGCAGCTGCAACACCTTGA

Coding sequence (CDS)

ATGAATGTGTCCACCATGTTCATGAGCAAAGAGATGATCACTCAACCATTGATAAATCTTCCTCCTCGAAGAATAAACTCCAACCCTTTCTTCAATCTCCCAAAGCTCGGAAAAGAGAAGGTGGTGATCGTGATCGGCGCTACTGGCACGGGTAAGTCTCGGTTGTCGATCGACATTGCTACCCGCTTCTCGATGGAAGTTATAAACTCTGATAAAATGCAAGTTTACGATGGGCTAGATATAGTTACAAATAAGGTCACGAAGAGAGAACAACGTGGTGTACCGCATCACTTGCTTGGAGTTATTGATCCCTTTTGTGACTTTTCGGCAGCTGACTTCTCTGATATGACTATCCGTGGTCGGCTCCCGATTATCGCTGGTGGCTCGAATTCTTATATTGAAACCTTAGTTGACTCGAACTTTGAATCGAGATATGAATGTTGCTTTATTTGGGTAGATGTGTCAATACCTGTATTGCAATCCTTTGTATCTAATAGGGTAGACAAAATGGTAGCTAAGGGAATGGTTGACGAGGCCGAAAAATTCTTTGATCCCACTCGAGATTATTCTCGAGGGATCAAAAGGGCAATTGGGGTTCCCGAATTCGACTCCTATTTTCGATACAAATCGTTCTTAAACGAGGAAACTCAAGAGAAACTCTTACAAGAATCCATATCAAAAGTGAAGGAGCACACATGCAAGCTAGCATTGAGGCAATTGGGAAAGATCCAACGACTGAGAAACACCAAAGAATGGAACATACACAGATTGGATGCTACAGAGGCCTTTACAAAGCGAGGCAAAGAAGCAGAAGAAGCATGGAACAAGCTCGTCACGACACGAAGCACAACGATAATCGACCAATTTCTTCATCCGAAGATCTTCACCGTCAAAACCCAGAAGAATTTAGCATCCCTCCCCGGCGTGCCGCCTAAGAAAAGTGCAGCCATTGCAGCTGCAACACCTTGA

Protein sequence

MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTIRGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVAKGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHTCKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHPKIFTVKTQKNLASLPGVPPKKSAAIAAATP
Homology
BLAST of CmaCh17G011650 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.8e-83
Identity = 161/304 (52.96%), Postives = 207/304 (68.09%), Query Frame = 0

Query: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           M +S     + +   P ++ PP R   N     P   K+KVV+++GATGTGKSRLS+DIA
Sbjct: 3   MKISMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIA 62

Query: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMT--- 120
           TRF  E+INSDK+QV+ GLDIVTNK+T  E  GVPHHLLGV+ P  D +AA++  M    
Sbjct: 63  TRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLS 122

Query: 121 -----IRGRLPIIAGGSNSYIETLVD---SNFESRYECCFIWVDVSIPVLQSFVSNRVDK 180
                 RG+LPII GGSNSY+E LVD   + F SRY+CCF+WVDV++PVL  FVS RVDK
Sbjct: 123 IESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDK 182

Query: 181 MVAKGMVDEAEKFFD-PTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKV 240
           MV  GMV+E  +FFD    DYSRGIK+AIG PEFD +FR + FLN E +E+LL + + ++
Sbjct: 183 MVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEI 242

Query: 241 KEHTCKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGK--EAEEAWNKLVTTRSTTII 291
           K +T +LA RQ  KI+RLR  K+W+I R+DAT  FTKR    +A  AW +LV   ST  +
Sbjct: 243 KRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTV 302

BLAST of CmaCh17G011650 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 297.7 bits (761), Expect = 1.5e-79
Identity = 160/275 (58.18%), Postives = 195/275 (70.91%), Query Frame = 0

Query: 29  PFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRFSMEVINSDKMQVYDGLDIVTNKVTK 88
           PFF      K+KVV V+GATGTGKSRL+ID+ATRF  E++NSDK+QVY GLDIVTNKVT 
Sbjct: 27  PFFR----RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTP 86

Query: 89  REQRGVPHHLLGVI-DPFCDFSAADFSDMTIRG--------RLPIIAGGSNSYIETLVDS 148
            E  GVPHHLLG + D + DF+A DF    IR         R+PIIAGGSNSYIE LV+ 
Sbjct: 87  EESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVND 146

Query: 149 --NFESRYECCFIWVDVSIPVLQSFVSNRVDKMVAKGMVDEAEKFFDP-TRDYSRGIKRA 208
             +F  RY CCF+WVDVS PVL SFVS RVDKMV  G+VDE  + FDP + DYS GI+RA
Sbjct: 147 CVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRA 206

Query: 209 IGVPEFDSYFRYK-SFLNEETQEKLLQESISKVKEHTCKLALRQLGKIQRLRNTKEWNIH 268
           IGVPE D + R +      ET E+LL+ +I K+KE+TC LA RQL KIQRL    +WN+H
Sbjct: 207 IGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMH 266

Query: 269 RLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFL 291
           R+DATE F +RG+EA+EAW+  V   S   +++FL
Sbjct: 267 RVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFL 297

BLAST of CmaCh17G011650 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 268.9 bits (686), Expect = 7.7e-71
Identity = 139/279 (49.82%), Postives = 192/279 (68.82%), Query Frame = 0

Query: 24  RINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRFSMEVINSDKMQVYDGLDIVT 83
           ++N N F +     KEKV+ V+GATG+GKSRL+ID+ATRF  E+INSDK+Q+Y GLD++T
Sbjct: 23  KVNVNSFLH----PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLT 82

Query: 84  NKVTKREQRGVPHHLLGVID---------PFCDFSAADFSDMTIRGRLPIIAGGSNSYIE 143
           NKVT +E RGVPHHLLGV D          +   ++   S ++   +LPI+AGGSNSYIE
Sbjct: 83  NKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIE 142

Query: 144 TLVDSN---FESRYECCFIWVDVSIPVLQSFVSNRVDKMVAKGMVDEAEKFFDPTRDYSR 203
            LV+ +     + Y+CCFIWVDVS+PVL SFVS RVD+M+  G+++E  + F+P  +YS 
Sbjct: 143 ALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSV 202

Query: 204 GIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHTCKLALRQLGKIQRLRNTKE 263
           GI+RAIGVPE   Y R +S ++  T+ K+L  ++  +K++T  LA RQL KIQRL    +
Sbjct: 203 GIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWK 262

Query: 264 WNIHRLDATEAFTKRG-KEAEEAWNKLVTTRSTTIIDQF 290
            ++HR+DATE F KR  +E +EAW  LV   S  I+D+F
Sbjct: 263 MSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKF 297

BLAST of CmaCh17G011650 vs. ExPASy Swiss-Prot
Match: Q9LJL4 (Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT8 PE=2 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 3.9e-59
Identity = 131/327 (40.06%), Postives = 197/327 (60.24%), Query Frame = 0

Query: 2   NVSTMFMSKEM--ITQPLINL-PPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSID 61
           N+++ F+S  M  IT P + L PPR +       + +  K+KVV+++GATG+GKS LSID
Sbjct: 3   NLTSTFVSPSMIPITSPRLRLPPPRSVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSID 62

Query: 62  IATRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDP---------FCDFS 121
           +ATRFS E++NSDK+Q YDGL + TN+++  E+ GVPHHLLG + P         F   +
Sbjct: 63  LATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSLA 122

Query: 122 AADFSDMTIRGRLPIIAGGSNSYIETLVDSNFES---------------RYECCFIWVDV 181
           +   S++T RG LPIIAGGSNS+I  L+   F+                RYECCF+WVDV
Sbjct: 123 SRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFSSETSISSGLRYECCFLWVDV 182

Query: 182 SIPVLQSFVSNRVDKMVAKGMVDEAEKFFDPTRDYS----RGIKRAIGVPEFDSYF---- 241
           S+ VL  ++S RVD+M+  GM +E   F+DP    S     GI + IG+PEFD YF    
Sbjct: 183 SVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYP 242

Query: 242 ---RYKSFLNEETQEKLLQESISKVKEHTCKLALRQLGKIQRLRNTKEWNIHRLDATEAF 291
              + K    ++ ++    E++ ++KE+T +LA +Q+ +I +L+++  W+I RLDAT +F
Sbjct: 243 PERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIERIMKLKSS-GWDIQRLDATPSF 302

BLAST of CmaCh17G011650 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.3e-58
Identity = 128/300 (42.67%), Postives = 181/300 (60.33%), Query Frame = 0

Query: 38  KEKVVIVIGATGTGKSRLSIDIATRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHH 97
           KEK+++++GATGTGKSRLSID+A  F +EVINSDKMQVY GLDI TNK++  ++ GVPHH
Sbjct: 29  KEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPHH 88

Query: 98  LLGVIDPF-CDFSAADF--------SDMTIRGRLPIIAGGSNSYIETLVDSNFES----- 157
           LLG +DP   + + ADF        S++T R +LP++ GGSNS+I  L+   F+S     
Sbjct: 89  LLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPGV 148

Query: 158 -------------RYECCFIWVDVSIPVLQSFVSNRVDKMVAKGMVDEAEKFFDP-TRDY 217
                        RY+CCF+WVDVS+ VL  +++ RVD M+  GM DE  +F+ P   D+
Sbjct: 149 FEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDEDH 208

Query: 218 SR------GIKRAIGVPEFDSYFRY--------KSFLNEETQEKLLQESISKVKEHTCKL 277
                   G+++AIGVPEFD YF          +    +  +    +E++  +KE+TC L
Sbjct: 209 DEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKENTCHL 268

Query: 278 ALRQLGKIQRLRNTKEWNIHRLDATEAF-----TKRGKEAEEAWNKLVTTRSTTIIDQFL 291
           A RQ+GKI RL+    W++ RLDATE+F     +  G++  E W K V   S  I+ +FL
Sbjct: 269 AKRQIGKILRLKGA-GWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRFL 327

BLAST of CmaCh17G011650 vs. ExPASy TrEMBL
Match: A0A6J1JXT3 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488830 PE=4 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 3.7e-177
Identity = 321/330 (97.27%), Postives = 322/330 (97.58%), Query Frame = 0

Query: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTI-- 120
           TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMT+  
Sbjct: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTMLS 120

Query: 121 ------RGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA 180
                 RGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA
Sbjct: 121 LDSIYSRGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA 180

Query: 181 KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT 240
           KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT
Sbjct: 181 KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT 240

Query: 241 CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP 300
           CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP
Sbjct: 241 CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP 300

Query: 301 KIFTVKTQKNLASLPGVPPKKSAAIAAATP 323
           KIFTVKTQKNLASLPGVPPKKSAAIAAATP
Sbjct: 301 KIFTVKTQKNLASLPGVPPKKSAAIAAATP 330

BLAST of CmaCh17G011650 vs. ExPASy TrEMBL
Match: A0A6J1GR83 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456395 PE=4 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 3.8e-166
Identity = 304/332 (91.57%), Postives = 313/332 (94.28%), Query Frame = 0

Query: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSKEMITQPLINLPPRRIN+NPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSKEMITQPLINLPPRRINTNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTI-- 120
           TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDM +  
Sbjct: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMAMLS 120

Query: 121 ------RGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA 180
                 RGRLPIIAGGSNS+IETLVDSNF+SRYECCF+WVDVSIPVLQSFVS+RVDKMVA
Sbjct: 121 LDSICSRGRLPIIAGGSNSFIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180

Query: 181 KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT 240
           KGMVDEAEKFFDP RDYSRGIKRAIGVPEFDSYFR KSFL+E+T+EKLLQESISKVKEHT
Sbjct: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCKSFLDEKTREKLLQESISKVKEHT 240

Query: 241 CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP 300
           CKLALRQLGKIQRLRN KEWNIHRLDATE FTKRGKEAEEAWNKLVT RSTTIIDQFLHP
Sbjct: 241 CKLALRQLGKIQRLRNIKEWNIHRLDATEVFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300

Query: 301 KIFTVKTQKNLASL--PGVPPKKSAAIAAATP 323
           KI TVKTQKNL SL  P VPPKKSAAIAAATP
Sbjct: 301 KIVTVKTQKNLESLPVPSVPPKKSAAIAAATP 332

BLAST of CmaCh17G011650 vs. ExPASy TrEMBL
Match: A0A6J1KSJ2 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111497849 PE=4 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 9.5e-141
Identity = 256/330 (77.58%), Postives = 287/330 (86.97%), Query Frame = 0

Query: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+++  QPLINLPPRRIN+NPFFN+ KL KEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTI-- 120
           T+F  EVINSDKMQVYDGLDIVTNKVTK+EQRGVPHHLLG+IDPF DFSA +FSDM +  
Sbjct: 61  TQFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 ------RGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA 180
                 R RLPIIAGGSNSYIE L+DSNF+S+YECCF+WVDVS+P+L SFVS RVDKMVA
Sbjct: 121 LDTILGRDRLPIIAGGSNSYIEALIDSNFQSKYECCFLWVDVSMPILHSFVSKRVDKMVA 180

Query: 181 KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT 240
            GMV+EA++FFDP  DYSRGIKRAIGVPEFDSYFRY+S L++ETQEKLLQE+ISKVK++T
Sbjct: 181 NGMVNEAKEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP 300
           CKLA RQ+GKIQRLRN K W IHRLDATEAF K+GKEAEE WNKLVT  STTII+QFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIEQFLHS 300

Query: 301 KIFTVKTQKNLASLPGVPPKKSAAIAAATP 323
            + T     NLASL G+P K+SAAIAAATP
Sbjct: 301 NVITTNVPTNLASLLGLPRKRSAAIAAATP 330

BLAST of CmaCh17G011650 vs. ExPASy TrEMBL
Match: A0A6J1H8C9 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460993 PE=4 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.6e-140
Identity = 257/330 (77.88%), Postives = 285/330 (86.36%), Query Frame = 0

Query: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+++  QPLINLPPRRIN+NPFFN+ KL KEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTI-- 120
           TRF  EVINSDKMQVYDGLDIVTNKVTK+EQRGVPHHLLG+IDPF DFSA +FSDM +  
Sbjct: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 ------RGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA 180
                 R RLPIIAGGSNSY+E L+DSNF+S+YECCF+WVDVS+PVL SFVS RVDKMVA
Sbjct: 121 LVTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180

Query: 181 KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT 240
            GMVDEAE+FFDP  DYSRGIKRAIGVPEFDSYFRY+S L++ETQEKLLQE+ISKVK++T
Sbjct: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP 300
           CKLA RQ+GKIQRLRN K W IHRLDATEAF K+GKEAEE WNKLVT  STTIIDQFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300

Query: 301 KIFTVKTQKNLASLPGVPPKKSAAIAAATP 323
            + T K   NLA+L  +   +SAAIAAATP
Sbjct: 301 NVITTKVPTNLATLLDLSRMRSAAIAAATP 330

BLAST of CmaCh17G011650 vs. ExPASy TrEMBL
Match: A0A5D3DBM7 (Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00080 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.5e-138
Identity = 258/330 (78.18%), Postives = 281/330 (85.15%), Query Frame = 0

Query: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+    QPLINLPP R+N+N FFNL KL KEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFFNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTI-- 120
           TRF  EVINSDKMQVY GLDIVTNK+TK+EQ GVPHHLLG+I+PF DFSA++FSDM +  
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 ------RGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA 180
                   RLPIIAGGSNSYIE L+DSNF  +YECCF+WVDVS+ VLQSFVS RVDKMVA
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT 240
            GMVDEAEKFFDP RDYSRGIKRAIGVPEFDSYFR +S L+EE QEKLL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP 300
           CKLAL+QLGKIQRLRN K WNIHRLDATEAFTK+GKEAEEAWNKLVT  STTIIDQFLHP
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 KIFTVKTQKNLASLPGVPPKKSAAIAAATP 323
                K   NLASL GVP K+SAA+AAATP
Sbjct: 301 NAVATKFPTNLASLIGVPRKRSAAMAAATP 330

BLAST of CmaCh17G011650 vs. NCBI nr
Match: XP_022992534.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 630.6 bits (1625), Expect = 7.6e-177
Identity = 321/330 (97.27%), Postives = 322/330 (97.58%), Query Frame = 0

Query: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTI-- 120
           TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMT+  
Sbjct: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTMLS 120

Query: 121 ------RGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA 180
                 RGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA
Sbjct: 121 LDSIYSRGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA 180

Query: 181 KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT 240
           KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT
Sbjct: 181 KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT 240

Query: 241 CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP 300
           CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP
Sbjct: 241 CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP 300

Query: 301 KIFTVKTQKNLASLPGVPPKKSAAIAAATP 323
           KIFTVKTQKNLASLPGVPPKKSAAIAAATP
Sbjct: 301 KIFTVKTQKNLASLPGVPPKKSAAIAAATP 330

BLAST of CmaCh17G011650 vs. NCBI nr
Match: XP_023547390.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 602.1 bits (1551), Expect = 2.9e-168
Identity = 308/332 (92.77%), Postives = 316/332 (95.18%), Query Frame = 0

Query: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSKE+ITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSKEIITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTI-- 120
           TR+SMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSA DFSDMT+  
Sbjct: 61  TRYSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSATDFSDMTMLS 120

Query: 121 ------RGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA 180
                 RGRLPIIAGGSNSYIETLVDSNF+SRYECCF+WVDVSIPVLQSFVS+RVDKMVA
Sbjct: 121 LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180

Query: 181 KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT 240
           KGMVDEAEKFFDP RDYSRGIKRAIGVPEFDSYFRYKSFL+EET+EKLLQESISKVKEHT
Sbjct: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEETREKLLQESISKVKEHT 240

Query: 241 CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP 300
           CKLALRQLGKIQRL N KEWNIHRLDATEAFTKRGKEAEEAWNKLVT RSTTIIDQFLHP
Sbjct: 241 CKLALRQLGKIQRLINIKEWNIHRLDATEAFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300

Query: 301 KIFTVKTQKNLASL--PGVPPKKSAAIAAATP 323
           KI TVKTQKNLASL  PGVPPKKSAAIAAATP
Sbjct: 301 KIVTVKTQKNLASLPGPGVPPKKSAAIAAATP 332

BLAST of CmaCh17G011650 vs. NCBI nr
Match: KAG7014428.1 (Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 600.5 bits (1547), Expect = 8.5e-168
Identity = 308/332 (92.77%), Postives = 315/332 (94.88%), Query Frame = 0

Query: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTI-- 120
           TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDM +  
Sbjct: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMAMLS 120

Query: 121 ------RGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA 180
                 RGRLPIIAGGSNSYIETLVDSNF+SRYECCF+WVDVSIPVLQSFVS+RVDKMVA
Sbjct: 121 LDSICSRGRLPIIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180

Query: 181 KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT 240
           KGMVDEAEKFFDP RDYSRGIKRAIGVPEFDSYFRYKSFL+E+T+EKLLQESISKVKEHT
Sbjct: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSFLDEKTREKLLQESISKVKEHT 240

Query: 241 CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP 300
           CKLALRQLGKIQRLRN KEWNIHRLDATE FTKRGKEAEEAWNKLVT RSTTIIDQFLHP
Sbjct: 241 CKLALRQLGKIQRLRNIKEWNIHRLDATEVFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300

Query: 301 KIFTVKTQKNLASL--PGVPPKKSAAIAAATP 323
           KI TVKTQKNLASL  PGVP KKSAAIAAATP
Sbjct: 301 KIVTVKTQKNLASLPGPGVPLKKSAAIAAATP 332

BLAST of CmaCh17G011650 vs. NCBI nr
Match: XP_022954005.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 594.0 bits (1530), Expect = 7.9e-166
Identity = 304/332 (91.57%), Postives = 313/332 (94.28%), Query Frame = 0

Query: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSKEMITQPLINLPPRRIN+NPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSKEMITQPLINLPPRRINTNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTI-- 120
           TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDM +  
Sbjct: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMAMLS 120

Query: 121 ------RGRLPIIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVA 180
                 RGRLPIIAGGSNS+IETLVDSNF+SRYECCF+WVDVSIPVLQSFVS+RVDKMVA
Sbjct: 121 LDSICSRGRLPIIAGGSNSFIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVA 180

Query: 181 KGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHT 240
           KGMVDEAEKFFDP RDYSRGIKRAIGVPEFDSYFR KSFL+E+T+EKLLQESISKVKEHT
Sbjct: 181 KGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCKSFLDEKTREKLLQESISKVKEHT 240

Query: 241 CKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHP 300
           CKLALRQLGKIQRLRN KEWNIHRLDATE FTKRGKEAEEAWNKLVT RSTTIIDQFLHP
Sbjct: 241 CKLALRQLGKIQRLRNIKEWNIHRLDATEVFTKRGKEAEEAWNKLVTARSTTIIDQFLHP 300

Query: 301 KIFTVKTQKNLASL--PGVPPKKSAAIAAATP 323
           KI TVKTQKNL SL  P VPPKKSAAIAAATP
Sbjct: 301 KIVTVKTQKNLESLPVPSVPPKKSAAIAAATP 332

BLAST of CmaCh17G011650 vs. NCBI nr
Match: KAG6575896.1 (Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 544.3 bits (1401), Expect = 7.2e-151
Identity = 273/294 (92.86%), Postives = 280/294 (95.24%), Query Frame = 0

Query: 12  MITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRFSMEVINSD 71
           MITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRFSMEVINSD
Sbjct: 1   MITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRFSMEVINSD 60

Query: 72  KMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMTI--------RGRLP 131
           KMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDM +        RGRLP
Sbjct: 61  KMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMAMLSLDSICSRGRLP 120

Query: 132 IIAGGSNSYIETLVDSNFESRYECCFIWVDVSIPVLQSFVSNRVDKMVAKGMVDEAEKFF 191
           IIAGGSNSYIETLVDSNF+SRYECCF+WVDVSIPVLQSFVS+RVDKMVAKGMVDEAEKFF
Sbjct: 121 IIAGGSNSYIETLVDSNFQSRYECCFLWVDVSIPVLQSFVSDRVDKMVAKGMVDEAEKFF 180

Query: 192 DPTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHTCKLALRQLGKI 251
           DP RDYSRGIKRAIGVPEFDSYFRYKSFL+E+T+EKLLQESISKVKEHTCKLALRQLGKI
Sbjct: 181 DPARDYSRGIKRAIGVPEFDSYFRYKSFLDEKTREKLLQESISKVKEHTCKLALRQLGKI 240

Query: 252 QRLRNTKEWNIHRLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFLHPKIFTV 298
           QRLRN KEWNIHRLDATE FTKRGKEAEEAWNKLVT RSTTIIDQFLHPKI TV
Sbjct: 241 QRLRNIKEWNIHRLDATEVFTKRGKEAEEAWNKLVTARSTTIIDQFLHPKIVTV 294

BLAST of CmaCh17G011650 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 310.8 bits (795), Expect = 1.2e-84
Identity = 161/304 (52.96%), Postives = 207/304 (68.09%), Query Frame = 0

Query: 1   MNVSTMFMSKEMITQPLINLPPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIA 60
           M +S     + +   P ++ PP R   N     P   K+KVV+++GATGTGKSRLS+DIA
Sbjct: 3   MKISMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIA 62

Query: 61  TRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDPFCDFSAADFSDMT--- 120
           TRF  E+INSDK+QV+ GLDIVTNK+T  E  GVPHHLLGV+ P  D +AA++  M    
Sbjct: 63  TRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLS 122

Query: 121 -----IRGRLPIIAGGSNSYIETLVD---SNFESRYECCFIWVDVSIPVLQSFVSNRVDK 180
                 RG+LPII GGSNSY+E LVD   + F SRY+CCF+WVDV++PVL  FVS RVDK
Sbjct: 123 IESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDK 182

Query: 181 MVAKGMVDEAEKFFD-PTRDYSRGIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKV 240
           MV  GMV+E  +FFD    DYSRGIK+AIG PEFD +FR + FLN E +E+LL + + ++
Sbjct: 183 MVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEI 242

Query: 241 KEHTCKLALRQLGKIQRLRNTKEWNIHRLDATEAFTKRGK--EAEEAWNKLVTTRSTTII 291
           K +T +LA RQ  KI+RLR  K+W+I R+DAT  FTKR    +A  AW +LV   ST  +
Sbjct: 243 KRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTV 302

BLAST of CmaCh17G011650 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 297.7 bits (761), Expect = 1.1e-80
Identity = 160/275 (58.18%), Postives = 195/275 (70.91%), Query Frame = 0

Query: 29  PFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRFSMEVINSDKMQVYDGLDIVTNKVTK 88
           PFF      K+KVV V+GATGTGKSRL+ID+ATRF  E++NSDK+QVY GLDIVTNKVT 
Sbjct: 27  PFFR----RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTP 86

Query: 89  REQRGVPHHLLGVI-DPFCDFSAADFSDMTIRG--------RLPIIAGGSNSYIETLVDS 148
            E  GVPHHLLG + D + DF+A DF    IR         R+PIIAGGSNSYIE LV+ 
Sbjct: 87  EESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVND 146

Query: 149 --NFESRYECCFIWVDVSIPVLQSFVSNRVDKMVAKGMVDEAEKFFDP-TRDYSRGIKRA 208
             +F  RY CCF+WVDVS PVL SFVS RVDKMV  G+VDE  + FDP + DYS GI+RA
Sbjct: 147 CVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRA 206

Query: 209 IGVPEFDSYFRYK-SFLNEETQEKLLQESISKVKEHTCKLALRQLGKIQRLRNTKEWNIH 268
           IGVPE D + R +      ET E+LL+ +I K+KE+TC LA RQL KIQRL    +WN+H
Sbjct: 207 IGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMH 266

Query: 269 RLDATEAFTKRGKEAEEAWNKLVTTRSTTIIDQFL 291
           R+DATE F +RG+EA+EAW+  V   S   +++FL
Sbjct: 267 RVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFL 297

BLAST of CmaCh17G011650 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 268.9 bits (686), Expect = 5.4e-72
Identity = 139/279 (49.82%), Postives = 192/279 (68.82%), Query Frame = 0

Query: 24  RINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSIDIATRFSMEVINSDKMQVYDGLDIVT 83
           ++N N F +     KEKV+ V+GATG+GKSRL+ID+ATRF  E+INSDK+Q+Y GLD++T
Sbjct: 23  KVNVNSFLH----PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLT 82

Query: 84  NKVTKREQRGVPHHLLGVID---------PFCDFSAADFSDMTIRGRLPIIAGGSNSYIE 143
           NKVT +E RGVPHHLLGV D          +   ++   S ++   +LPI+AGGSNSYIE
Sbjct: 83  NKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIE 142

Query: 144 TLVDSN---FESRYECCFIWVDVSIPVLQSFVSNRVDKMVAKGMVDEAEKFFDPTRDYSR 203
            LV+ +     + Y+CCFIWVDVS+PVL SFVS RVD+M+  G+++E  + F+P  +YS 
Sbjct: 143 ALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSV 202

Query: 204 GIKRAIGVPEFDSYFRYKSFLNEETQEKLLQESISKVKEHTCKLALRQLGKIQRLRNTKE 263
           GI+RAIGVPE   Y R +S ++  T+ K+L  ++  +K++T  LA RQL KIQRL    +
Sbjct: 203 GIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWK 262

Query: 264 WNIHRLDATEAFTKRG-KEAEEAWNKLVTTRSTTIIDQF 290
            ++HR+DATE F KR  +E +EAW  LV   S  I+D+F
Sbjct: 263 MSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKF 297

BLAST of CmaCh17G011650 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 229.9 bits (585), Expect = 2.8e-60
Identity = 131/327 (40.06%), Postives = 197/327 (60.24%), Query Frame = 0

Query: 2   NVSTMFMSKEM--ITQPLINL-PPRRINSNPFFNLPKLGKEKVVIVIGATGTGKSRLSID 61
           N+++ F+S  M  IT P + L PPR +       + +  K+KVV+++GATG+GKS LSID
Sbjct: 3   NLTSTFVSPSMIPITSPRLRLPPPRSVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSID 62

Query: 62  IATRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDP---------FCDFS 121
           +ATRFS E++NSDK+Q YDGL + TN+++  E+ GVPHHLLG + P         F   +
Sbjct: 63  LATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSLA 122

Query: 122 AADFSDMTIRGRLPIIAGGSNSYIETLVDSNFES---------------RYECCFIWVDV 181
           +   S++T RG LPIIAGGSNS+I  L+   F+                RYECCF+WVDV
Sbjct: 123 SRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFSSETSISSGLRYECCFLWVDV 182

Query: 182 SIPVLQSFVSNRVDKMVAKGMVDEAEKFFDPTRDYS----RGIKRAIGVPEFDSYF---- 241
           S+ VL  ++S RVD+M+  GM +E   F+DP    S     GI + IG+PEFD YF    
Sbjct: 183 SVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYP 242

Query: 242 ---RYKSFLNEETQEKLLQESISKVKEHTCKLALRQLGKIQRLRNTKEWNIHRLDATEAF 291
              + K    ++ ++    E++ ++KE+T +LA +Q+ +I +L+++  W+I RLDAT +F
Sbjct: 243 PERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIERIMKLKSS-GWDIQRLDATPSF 302

BLAST of CmaCh17G011650 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 217.6 bits (553), Expect = 1.4e-56
Identity = 128/333 (38.44%), Postives = 192/333 (57.66%), Query Frame = 0

Query: 4   STMFMSKEMITQPLINLPPRRINSNPFFNLPKL-------GKEKVVIVIGATGTGKSRLS 63
           S  F S    +  L++   RR    P  +  ++        K+KVV+++GATG GKSRLS
Sbjct: 23  SPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLS 82

Query: 64  IDIATRFSMEVINSDKMQVYDGLDIVTNKVTKREQRGVPHHLLGVIDP-FCDFSAADF-- 123
           +D+ATRF  E+INSDK+QVY+GL+I TN++T +++RGVPHHLLGVI+P   + +A +F  
Sbjct: 83  VDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRS 142

Query: 124 ------SDMTIRGRLPIIAGGSNSYIETLVDSNFES----------------RYECCFIW 183
                  ++T R ++PIIAGGSNS++  L+   F+                 RYECCFIW
Sbjct: 143 AASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIW 202

Query: 184 VDVSIPVLQSFVSNRVDKMVAKGMVDEAEKFFDPTR---DYSRGIKRAIGVPEFDSYFR- 243
           VDVS  VL  ++  RVD+M+  GM +E  +F+DP +   +   GI++AIGVPEFD YF+ 
Sbjct: 203 VDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKE 262

Query: 244 ----YKSFLNEETQEKLLQESISKVKEHTCKLALRQLGKIQRLRNTKEWNIHRLDATEAF 291
                K    +  ++    +++  +K +T  LA RQ+ KI+ L++   W I R+DAT +F
Sbjct: 263 YPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDA-GWEIERVDATASF 322

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93WC91.8e-8352.96Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID21.5e-7958.18Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID17.7e-7149.82Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Q9LJL43.9e-5940.06Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q5GHF73.3e-5842.67Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JXT33.7e-17797.27adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
A0A6J1GR833.8e-16691.57adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A6J1KSJ29.5e-14177.58adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
A0A6J1H8C91.6e-14077.88adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A5D3DBM71.5e-13878.18Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
XP_022992534.17.6e-17797.27adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima][more]
XP_023547390.12.9e-16892.77adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pe... [more]
KAG7014428.18.5e-16892.77Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosper... [more]
XP_022954005.17.9e-16691.57adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita moschata][more]
KAG6575896.17.2e-15192.86Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
AT3G63110.11.2e-8452.96isopentenyltransferase 3 [more]
AT5G19040.11.1e-8058.18isopentenyltransferase 5 [more]
AT3G23630.15.4e-7249.82isopentenyltransferase 7 [more]
AT3G19160.12.8e-6040.06ATP/ADP isopentenyltransferases [more]
AT1G68460.11.4e-5638.44isopentenyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 38..290
e-value: 1.0E-100
score: 338.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 37..183
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 156..254
e-value: 1.0E-100
score: 338.4
NoneNo IPR availablePFAMPF01715IPPTcoord: 143..240
e-value: 6.6E-11
score: 42.4
coord: 74..139
e-value: 2.5E-16
score: 60.1
NoneNo IPR availablePANTHERPTHR11088:SF74ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTICcoord: 36..294
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 36..294

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G011650.1CmaCh17G011650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009691 cytokinin biosynthetic process
biological_process GO:0006400 tRNA modification
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005634 nucleus
cellular_component GO:0009536 plastid
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0052381 tRNA dimethylallyltransferase activity