CmaCh17G011260 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G011260
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionE3 ubiquitin-protein ligase MIEL1-like
LocationCma_Chr17: 7858274 .. 7861573 (+)
RNA-Seq ExpressionCmaCh17G011260
SyntenyCmaCh17G011260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATTTTTAGTTTTGCAATTATTCTCAAACGAAGCCGAAGAGCTCGAACAGGGAGACGGTCTCCAATGGAACAAGAGGACTCCCCTGTGCCCCAATCTGCCGGGAGAGAGGATTTCGGGAAATTTCAGCACGGGTATCGATTTCTGTATCATGTCTTCTTCTTCTTCTTTGTTCTCGCTTTTGTTATTAGTAACTTGTTTTCGATTTCAATTAGATGCGAGCATTATAGGAGGCGTTGCAGAATCCGAGCCCCATGCTGCGACCGAATCTTTACCTGTCGCCATTGCCACAACGAGGCCATGGTATGTCTCTCTTCTTTCTAGGCCCTTGTGTTGCTTGCGTTTGTTTGTGAGAGATGTATTAGAAAGGATTTTGCATATGCTGTTAAACTCGAACTTTCTGTAATTTTCTCTAGTAGTTTTCTGAATTTTCTGAAATTTTCGTTGATTTTAGACTTCGCTGAGCAATCCTAAGGACCGTCACGATCTCGTTCGGCAGGACGTTAGACAAGTCTGTTTGTACGCTGTTCTATTGTTTTTTTTTTTTTTTTCTTCTTTTTTGTGATATAACATCTTTTAGAGCATATCGTGATGTGAATTTATTAATTTTGTTGGAATTTTTGCTTTAGGTGGTTTGCTTGATTTGCAACACAGAGCAAGAGGTTTGGGGGACATTTTTTTTGTTGTTCTTGGGATAATTTCCATTCATTGCGTTTAGATATTCTTGTTTTTCGCTCTGCTAAGCGTTTATATTTGGTTTATTATAGGTTTCCAAGTCTTGTAGCAAGTGCAATGTTAATATGGGAGAATACTTCTGCGGCATCTGCAAATTCTACGATGATGATGTAGGAGAAGTTTCCATCAATCTGTTTCTTTCTTACTTTGTTCTGTTTCACTTTCTTTAAGAACTAATTTTCAGTTTTAAACTGTGGAACCGAATGGTTTTCTTATCCTAGATGCTAATAGATTCTGCTATGAATGCAGACCAGGAAAAAGCAGTTTCATTGTGATGCGTGTGGTATCTGTAGGTTCGTGTGCATATTTCCCGTTCTAGTTCTAATGTAAACTCAGCTTTCTGTGCTAAGTTTCTCAACACATGTGTTCTTTGTTATATGATTTTCAGGGTTGGTGGTCGTGAAAATTTCTTTCACTGTGAAAAATGTGGTACGTCATTCAATTCTCCTGATGGTTTGTTAGAAATACACATTGTGTTATTCATTTACTGTTTGAGGTAAATGTAGCAAAAGGATTTCTCTGGTTTGTCTGTAAAGTCTTTGATACTATGTAATAAAACAGGCACACACTTTGAGGCCATATGAGAACGAAACAATTAGTAAAATGTGAGCAATTGAGCGAATAAACCGTTCAGATGAATTAACTTTTTTTTTTTTTTATAAATACAGGATCTTGCTATTCCACTCCTCTGCGTGATAACCACATGTGTGTTGAGAACTCCATGAAAAGTTTTTGCCCCATCTGTTATGAGGTTTGTCCTTTTAGAATGCTTCTTTTCTCAGGGAAGTATAATCTAAACTGTTCTCTTCTAACAATTTTATTGATGCTTGCAGTTTCTTTTTGACTCAATAAAAGACACAACTGTTATGCCTTGTGGTCACACAATACATTTGGAGTGCTTCAGAGAGATGGAATCACAGAATCAGTAATCCAAACTCCTAATTCAAAATCCAGTTTCTGAATCTACTATAAAACATGTTTCTAAAAACTTTGAATATGCTACATACAACTCTATTTTCTATTATATCCATTGCCTTTTATTCTCAAACCAAACTGGTTCTAAGTTTCCTGTATTGAATTACATTAAGATTTCCAGTTCTCTTGTAATAATGAAGTTGTATCTGTCTCTCAGATACCGTTGTCCTATTTGCTCAAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGGTGTGTTACATCTTAGTTTAGGCGTGATAAAAGTGGCATGTAGAAGGCAATTAGATTACTATCTTTTTTATCCATAGAGCAGTTTCCCCAGTTAGTGAATATGGTAGAACAGGTGGCTTCACATAAATTTTAGCAACCTTAGAAATTAGAATATATATCTTATCATTGAACATTGTACCCACTCTTCAACCTCCTGACCGATCTTTTCGTTTCTTTCTTTGTCTTGATGTATGATTACATGTATTGGGCTGATTCTTTGAAAGTTGATGGTTGCAGACTCAATATCTCAATAGAATTCTAACTGAGTGATTGCATGAAGAAAGAGATTTAAGAAATGAGTGTGGATTCAGGGCTTTAGTTATGCCTTAAAGTTGAGTTTAGAGGGATATAGAGGCATTAACATATATAATAAAAATGAAAGAGGAAGATGTCCGTGAGAGCTATATATATTCTGTTACAGACAAGTACTTGACTTGATCTTATACGACCTTAAATCATGTTCGTCCACTTGAATAAAATTTTCTCTTATTTTTATTTTTCCCTCATTTCCCATTTACCCATTACCTGTTATCAAGTTGTTATTGGATGGTAAAATAGATCTTATTGGATGCTTACTTTTTGGTATTCCAAGTGGTATTTCTATCTATTTGCAAATTCTGACATGTTTCTTCTGCCATATTCTGGTGTCGAAATTCCAGAGATTTTTGGTTATCTTAATTATGTTTTCTTTACCATAGATCTTTTGCGATATTATCTTAAATTTGAAAATCGTTTCTATCTTATCTAGATTGACTGCACACCGATGCCGGAGGAGTACAGCCACGAGGTAGTTGCTTGTATACATGTTTAGATTACCGCAAGAATGAAATCTTCTAACTTAACTGTGTACTTGGCTAAACAGGTATCAATTCTTTGCAACGACTGCAGCCATACGAGCAAAGTTCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGGAGACGTCAATGAAAACTGAAGGTAAAGAAGTATGGAGCTCTCTTAATCTCAACAACTAGGTTATGTTGAACGTTTGATTATTGGAGTCATTGATCCATTTAGGAAGTCAACTATGATTGGTTTATGCCTATGAATTCAGTCACATGGAGTTAAGCAGTGCGATCTTCTATAGGAAGAGCCTCAGATTTTTTTTTTTTTCATTGTAACATTTAGCATTCATCTTGAAAAATATATATCATATTAGATACATTTTACAGATGTGAATATAACATGAATCTCAACAAATACTTGTTTGAGTACTTGGATTTCAAGTATAACAAGAGATAGATAAATGAATGATGATTTCCATTAAA

mRNA sequence

CAATTTTTAGTTTTGCAATTATTCTCAAACGAAGCCGAAGAGCTCGAACAGGGAGACGGTCTCCAATGGAACAAGAGGACTCCCCTGTGCCCCAATCTGCCGGGAGAGAGGATTTCGGGAAATTTCAGCACGGATGCGAGCATTATAGGAGGCGTTGCAGAATCCGAGCCCCATGCTGCGACCGAATCTTTACCTGTCGCCATTGCCACAACGAGGCCATGACTTCGCTGAGCAATCCTAAGGACCGTCACGATCTCGTTCGGCAGGACGTTAGACAAGTGGTTTGCTTGATTTGCAACACAGAGCAAGAGGTTTCCAAGTCTTGTAGCAAGTGCAATGTTAATATGGGAGAATACTTCTGCGGCATCTGCAAATTCTACGATGATGATACCAGGAAAAAGCAGTTTCATTGTGATGCGTGTGGTATCTGTAGGGTTGGTGGTCGTGAAAATTTCTTTCACTGTGAAAAATGTGGATCTTGCTATTCCACTCCTCTGCGTGATAACCACATGTGTGTTGAGAACTCCATGAAAAGTTTTTGCCCCATCTGTTATGAGTTTCTTTTTGACTCAATAAAAGACACAACTGTTATGCCTTGTGGTCACACAATACATTTGGAGTGCTTCAGAGAGATGGAATCACAGAATCAATACCGTTGTCCTATTTGCTCAAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGATTGACTGCACACCGATGCCGGAGGAGTACAGCCACGAGGTATCAATTCTTTGCAACGACTGCAGCCATACGAGCAAAGTTCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGGAGACGTCAATGAAAACTGAAGGTAAAGAAGTATGGAGCTCTCTTAATCTCAACAACTAGGTTATGTTGAACGTTTGATTATTGGAGTCATTGATCCATTTAGGAAGTCAACTATGATTGGTTTATGCCTATGAATTCAGTCACATGGAGTTAAGCAGTGCGATCTTCTATAGGAAGAGCCTCAGATTTTTTTTTTTTTCATTGTAACATTTAGCATTCATCTTGAAAAATATATATCATATTAGATACATTTTACAGATGTGAATATAACATGAATCTCAACAAATACTTGTTTGAGTACTTGGATTTCAAGTATAACAAGAGATAGATAAATGAATGATGATTTCCATTAAA

Coding sequence (CDS)

ATGGAACAAGAGGACTCCCCTGTGCCCCAATCTGCCGGGAGAGAGGATTTCGGGAAATTTCAGCACGGATGCGAGCATTATAGGAGGCGTTGCAGAATCCGAGCCCCATGCTGCGACCGAATCTTTACCTGTCGCCATTGCCACAACGAGGCCATGACTTCGCTGAGCAATCCTAAGGACCGTCACGATCTCGTTCGGCAGGACGTTAGACAAGTGGTTTGCTTGATTTGCAACACAGAGCAAGAGGTTTCCAAGTCTTGTAGCAAGTGCAATGTTAATATGGGAGAATACTTCTGCGGCATCTGCAAATTCTACGATGATGATACCAGGAAAAAGCAGTTTCATTGTGATGCGTGTGGTATCTGTAGGGTTGGTGGTCGTGAAAATTTCTTTCACTGTGAAAAATGTGGATCTTGCTATTCCACTCCTCTGCGTGATAACCACATGTGTGTTGAGAACTCCATGAAAAGTTTTTGCCCCATCTGTTATGAGTTTCTTTTTGACTCAATAAAAGACACAACTGTTATGCCTTGTGGTCACACAATACATTTGGAGTGCTTCAGAGAGATGGAATCACAGAATCAATACCGTTGTCCTATTTGCTCAAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGATTGACTGCACACCGATGCCGGAGGAGTACAGCCACGAGGTATCAATTCTTTGCAACGACTGCAGCCATACGAGCAAAGTTCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGGAGACGTCAATGAAAACTGA

Protein sequence

MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKDRHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN
Homology
[Close]
BLAST of CmaCh17G011260 vs. ExPASy Swiss-Prot
Match: Q8VZK0 (E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 5.3e-105
Identity = 162/254 (63.78%), Postives = 203/254 (79.92%), Query Frame = 0 

Query: 14  REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKDRHDLVRQDVRQVV 73
           R  FGK   GC+HY+RRC+IRAPCC+ +F CRHCHNE+ ++L N  DRHDLVRQDV+QV+
Sbjct: 8   RLHFGKMGFGCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLRNIYDRHDLVRQDVKQVI 67

Query: 74  CLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFHC 133
           C +C+TEQ  ++ CS C VNMGEYFC IC FYDDDT K+QFHCD CGICRVGGRENFFHC
Sbjct: 68  CSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGGRENFFHC 127

Query: 134 EKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQ 193
           +KCGSCY+  LR+NH CVENSM+  CPICYE+LFDS+KDT VM CGHT+H+EC+ EM  +
Sbjct: 128 KKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVECYNEMIKR 187

Query: 194 NQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEY-SHEVSILCNDCSHTSKVRFHILGH 253
           +++ CPICS++V+DMS +W  L  EI+ T MP +Y   +V ILCNDC+ T++V FHI+G 
Sbjct: 188 DKFCCPICSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEVHFHIIGQ 247

Query: 254 KCSQCNSFNTRRIS 267
           KC  C S+NTR I+
Sbjct: 248 KCGHCRSYNTRAIA 261

[Close]
BLAST of CmaCh17G011260 vs. ExPASy Swiss-Prot
Match: Q9FFB6 (E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=1 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 2.5e-91
Identity = 144/267 (53.93%), Postives = 185/267 (69.29%), Query Frame = 0 

Query: 4   EDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLS-NPKDRH 63
           E+    + A   + G   +GC HYRRRC+IRAPCCD IF CRHCHNEA  SL      RH
Sbjct: 7   ENEQNQEFANLMEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRH 66

Query: 64  DLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGIC 123
           +L R +V +V+C +C TEQ+V ++CS C V MG+YFC  CKF+DDD  KKQ+HCD CGIC
Sbjct: 67  ELPRHEVSKVICSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGIC 126

Query: 124 RVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTI 183
           R GG ENFFHC++C  CYS  + D H CVE +M   CP+C+E+LFDS +D TV+ CGHT+
Sbjct: 127 RTGGEENFFHCKRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTM 186

Query: 184 HLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSH 243
           HLEC ++M   N+Y CP+CSK++ DMS  W  L  E+   PMP+ Y ++ V ILCNDC  
Sbjct: 187 HLECTKDMGLHNRYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGS 246

Query: 244 TSKVRFHILGHKCSQCNSFNTRRISTG 269
            + VRFH++ HKCS C S+NTR+   G
Sbjct: 247 NTNVRFHLIAHKCSSCGSYNTRQTQRG 273

[Close]
BLAST of CmaCh17G011260 vs. ExPASy Swiss-Prot
Match: Q5JL96 (Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=39947 GN=RZFP34 PE=2 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 5.3e-89
Identity = 142/265 (53.58%), Postives = 188/265 (70.94%), Query Frame = 0 

Query: 2   EQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLS-NPKD 61
           E+ +   P    + + G  Q+GC HYRRRCRIRAPCC+ IF CRHCHNE   S+  +   
Sbjct: 39  EKTEDVDPDDYEKLEEGIMQYGCAHYRRRCRIRAPCCNEIFDCRHCHNETKNSIKIDAVK 98

Query: 62  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 121
           RH+L R +V+QV+C +C TEQEV + C  C V MG+YFC +CK +DDD  K+Q+HC+ CG
Sbjct: 99  RHELPRHEVQQVICSLCGTEQEVRQVCISCGVCMGKYFCEVCKLFDDDVSKQQYHCNGCG 158

Query: 122 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 181
           ICR+GG+ENFFHC KCG CYS  L+++H CVE +M   CPIC+E+LF+S  D +V+PCGH
Sbjct: 159 ICRIGGKENFFHCSKCGCCYSIVLKNSHACVEGAMHHDCPICFEYLFESTNDVSVLPCGH 218

Query: 182 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 241
           TIH++C REME   Q+ CP+CSK+V DMS +W  L  E+  T      +  V ILCNDC 
Sbjct: 219 TIHVKCLREMEEHCQFACPLCSKSVCDMSKAWERLDEEL-ATISDTCDNKMVRILCNDCG 278

Query: 242 HTSKVRFHILGHKCSQCNSFNTRRI 266
            TS+V+FH++ HKC +C S+NTR+I
Sbjct: 279 ATSEVQFHLIAHKCQKCKSYNTRQI 302

[Close]
BLAST of CmaCh17G011260 vs. ExPASy Swiss-Prot
Match: Q10LI1 (E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SRFP1 PE=1 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.0e-87
Identity = 139/255 (54.51%), Postives = 179/255 (70.20%), Query Frame = 0 

Query: 12  AGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKDRHDLVRQDVRQ 71
           A R  FG+   GC+HYRRRCRIRAPCC+ +F CRHCHNE      + KD H+L R  V  
Sbjct: 6   ASRHGFGRMGFGCKHYRRRCRIRAPCCNDVFHCRHCHNE------STKDGHELDRHAVES 65

Query: 72  VVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFF 131
           V+CL+C+TEQ V++ C  C V MGEYFC  CKF+DDD  ++ FHC  CGICRVGG++NFF
Sbjct: 66  VICLVCDTEQPVAQVCYNCGVCMGEYFCSACKFFDDDVDREHFHCQDCGICRVGGKDNFF 125

Query: 132 HCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREME 191
           HCEKCGSCYS  LRD H C+ENSMK+ CPICYE+LFDS+++T+V+ CGHT+HL+CF EM 
Sbjct: 126 HCEKCGSCYSVSLRDKHCCIENSMKNNCPICYEYLFDSLRETSVLRCGHTMHLQCFHEML 185

Query: 192 SQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSKVRFHILG 251
             +++ CPICS  + DM      L  EI+   +  +Y  +  I+CNDC  T++V   + G
Sbjct: 186 KHDKFSCPICSMPIFDMDKFLRALDAEIEANMLHIDYMGKGWIVCNDCRDTTQVYARVAG 245

Query: 252 HKCSQCNSFNTRRIS 267
           HKC  C S NT R++
Sbjct: 246 HKCCHCQSHNTCRVA 254

[Close]
BLAST of CmaCh17G011260 vs. ExPASy Swiss-Prot
Match: Q9CR50 (RING finger and CHY zinc finger domain-containing protein 1 OS=Mus musculus OX=10090 GN=Rchy1 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 4.1e-65
Identity = 112/251 (44.62%), Postives = 154/251 (61.35%), Query Frame = 0 

Query: 13  GREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKDRHDLVRQDVRQV 72
           G  +  +   GCEHY R C ++APCCD+++TCR CH        +  + H L R  V++V
Sbjct: 9   GVRNLAQGPRGCEHYDRACLLKAPCCDKLYTCRLCH--------DTNEDHQLDRFKVKEV 68

Query: 73  VCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFH 132
            C+ C   Q   ++C  C+   GEY+C IC  +D D  K+Q+HC++CGICR+G +E+FFH
Sbjct: 69  QCINCEKLQHAQQTCEDCSTLFGEYYCSICHLFDKD--KRQYHCESCGICRIGPKEDFFH 128

Query: 133 CEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMES 192
           C KC  C +T LR  H C+EN  +  CPIC E +  S     V+PCGH +H  C+ EM  
Sbjct: 129 CLKCNLCLTTNLRGKHKCIENVSRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLK 188

Query: 193 QNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSH-EVSILCNDCSHTSKVRFHILG 252
           +  YRCP+C  + +DM+  W  L  E+  TPMP EY +  V ILCNDC+  S V+FHILG
Sbjct: 189 EG-YRCPLCMHSALDMTRYWRQLDTEVAQTPMPSEYQNVTVDILCNDCNGRSTVQFHILG 248

Query: 253 HKCSQCNSFNT 263
            KC  C+S+NT
Sbjct: 249 MKCKLCDSYNT 248

[Close]
BLAST of CmaCh17G011260 vs. ExPASy TrEMBL
Match: A0A6J1JVS4 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita maxima OX=3661 GN=LOC111488790 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.6e-168
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG
Sbjct: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 274
           HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273

[Close]
BLAST of CmaCh17G011260 vs. ExPASy TrEMBL
Match: A0A6J1GP21 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita moschata OX=3662 GN=LOC111455836 PE=4 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 3.8e-167
Identity = 270/273 (98.90%), Postives = 272/273 (99.63%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQEV+KSC KCNVNMGEYFCGICKFYDDDTRKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 274
           HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273

[Close]
BLAST of CmaCh17G011260 vs. ExPASy TrEMBL
Match: A0A6J1D947 (E3 ubiquitin-protein ligase MIEL1 OS=Momordica charantia OX=3673 GN=LOC111018400 PE=4 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 5.6e-158
Identity = 255/269 (94.80%), Postives = 263/269 (97.77%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           ME+EDS VPQSA REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQEV+K C KCNVNMGEYFC ICKF+DDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNH+CVENSMKSFCPICYEFLFDSIKDTTVM CGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHL+CFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 270
           HTSKVRFHILGHKCSQCNSFNTRRISTGD
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269

[Close]
BLAST of CmaCh17G011260 vs. ExPASy TrEMBL
Match: A0A6J1KQY2 (E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita maxima OX=3661 GN=LOC111497863 PE=4 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 2.3e-156
Identity = 252/267 (94.38%), Postives = 257/267 (96.25%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQ+D PVPQSAGREDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQE  + C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 268
           HTS VRFHILGHKCSQCNSFNTRRIST
Sbjct: 241 HTSNVRFHILGHKCSQCNSFNTRRIST 267

[Close]
BLAST of CmaCh17G011260 vs. ExPASy TrEMBL
Match: A0A6J1H4Y6 (E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita moschata OX=3662 GN=LOC111460617 PE=4 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 2.6e-155
Identity = 250/267 (93.63%), Postives = 256/267 (95.88%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQ+D PVPQSAGREDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAM+SLSNP D
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPND 60

Query: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQE  + C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 268
           HTS VRFHILGHKCS+CNSFNTRRIST
Sbjct: 241 HTSNVRFHILGHKCSRCNSFNTRRIST 267

[Close]
BLAST of CmaCh17G011260 vs. NCBI nr
Match: XP_022992470.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita maxima])

HSP 1 Score: 601.7 bits (1550), Expect = 3.2e-168
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG
Sbjct: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 274
           HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273

[Close]
BLAST of CmaCh17G011260 vs. NCBI nr
Match: XP_022953225.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita moschata] >KAG6575860.1 E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 597.0 bits (1538), Expect = 7.9e-167
Identity = 270/273 (98.90%), Postives = 272/273 (99.63%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQEV+KSC KCNVNMGEYFCGICKFYDDDTRKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 274
           HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273

[Close]
BLAST of CmaCh17G011260 vs. NCBI nr
Match: XP_023549279.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 596.7 bits (1537), Expect = 1.0e-166
Identity = 270/273 (98.90%), Postives = 272/273 (99.63%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQEV+KSCSKCNVNMGEYFCGICKFYDDDTRK QFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKSCSKCNVNMGEYFCGICKFYDDDTRKMQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 274
           HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273

[Close]
BLAST of CmaCh17G011260 vs. NCBI nr
Match: XP_022150148.1 (E3 ubiquitin-protein ligase MIEL1 [Momordica charantia] >XP_022150149.1 E3 ubiquitin-protein ligase MIEL1 [Momordica charantia])

HSP 1 Score: 566.6 bits (1459), Expect = 1.1e-157
Identity = 255/269 (94.80%), Postives = 263/269 (97.77%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           ME+EDS VPQSA REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQEV+K C KCNVNMGEYFC ICKF+DDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNH+CVENSMKSFCPICYEFLFDSIKDTTVM CGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHL+CFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 270
           HTSKVRFHILGHKCSQCNSFNTRRISTGD
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269

[Close]
BLAST of CmaCh17G011260 vs. NCBI nr
Match: XP_023004622.1 (E3 ubiquitin-protein ligase MIEL1 [Cucurbita maxima] >XP_023513585.1 E3 ubiquitin-protein ligase MIEL1 [Cucurbita pepo subsp. pepo] >KAG6593162.1 E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 561.2 bits (1445), Expect = 4.8e-156
Identity = 252/267 (94.38%), Postives = 257/267 (96.25%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQ+D PVPQSAGREDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQE  + C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 268
           HTS VRFHILGHKCSQCNSFNTRRIST
Sbjct: 241 HTSNVRFHILGHKCSQCNSFNTRRIST 267

[Close]
BLAST of CmaCh17G011260 vs. TAIR 10
Match: AT3G62970.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 413.3 bits (1061), Expect = 1.5e-115
Identity = 177/263 (67.30%), Postives = 213/263 (80.99%), Query Frame = 0 

Query: 5   DSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKDRHDL 64
           DS +P+    +DFGKFQ GCEHY+RRC+IRAPCC+ IF+CRHCHN++  SL +PK+RHDL
Sbjct: 19  DSSIPRD---KDFGKFQFGCEHYKRRCKIRAPCCNLIFSCRHCHNDSANSLPDPKERHDL 78

Query: 65  VRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRV 124
           VRQ+V+QVVC IC TEQEV+K CS C VNMGEYFC ICKF+DDD  K+QFHCD CGICRV
Sbjct: 79  VRQNVKQVVCSICQTEQEVAKVCSNCGVNMGEYFCDICKFFDDDISKEQFHCDDCGICRV 138

Query: 125 GGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHL 184
           GGR+ FFHC+ CG+CY   LRD H C+ENS K+ CP+CYE+LFDS+K   VM CGHT+H+
Sbjct: 139 GGRDKFFHCQNCGACYGMGLRDKHSCIENSTKNSCPVCYEYLFDSVKAAHVMKCGHTMHM 198

Query: 185 ECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSK 244
           +CF +M ++NQYRCPIC+K+++DMS SW LL  EI  T MP EY  EVSILCNDC+  SK
Sbjct: 199 DCFEQMINENQYRCPICAKSMVDMSPSWHLLDFEISATEMPVEYKFEVSILCNDCNKGSK 258

Query: 245 VRFHILGHKCSQCNSFNTRRIST 268
             FHILGHKCS C S+NTRRIST
Sbjct: 259 AMFHILGHKCSDCGSYNTRRIST 278

[Close]
BLAST of CmaCh17G011260 vs. TAIR 10
Match: AT5G18650.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 382.1 bits (980), Expect = 3.7e-106
Identity = 162/254 (63.78%), Postives = 203/254 (79.92%), Query Frame = 0 

Query: 14  REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKDRHDLVRQDVRQVV 73
           R  FGK   GC+HY+RRC+IRAPCC+ +F CRHCHNE+ ++L N  DRHDLVRQDV+QV+
Sbjct: 8   RLHFGKMGFGCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLRNIYDRHDLVRQDVKQVI 67

Query: 74  CLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFHC 133
           C +C+TEQ  ++ CS C VNMGEYFC IC FYDDDT K+QFHCD CGICRVGGRENFFHC
Sbjct: 68  CSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGGRENFFHC 127

Query: 134 EKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQ 193
           +KCGSCY+  LR+NH CVENSM+  CPICYE+LFDS+KDT VM CGHT+H+EC+ EM  +
Sbjct: 128 KKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVECYNEMIKR 187

Query: 194 NQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEY-SHEVSILCNDCSHTSKVRFHILGH 253
           +++ CPICS++V+DMS +W  L  EI+ T MP +Y   +V ILCNDC+ T++V FHI+G 
Sbjct: 188 DKFCCPICSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEVHFHIIGQ 247

Query: 254 KCSQCNSFNTRRIS 267
           KC  C S+NTR I+
Sbjct: 248 KCGHCRSYNTRAIA 261

[Close]
BLAST of CmaCh17G011260 vs. TAIR 10
Match: AT5G25560.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 339.7 bits (870), Expect = 2.1e-93
Identity = 146/251 (58.17%), Postives = 179/251 (71.31%), Query Frame = 0 

Query: 16  DFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLS-NPKDRHDLVRQDVRQVVC 75
           D G  ++GC HYRRRC IRAPCC+ IF C HCH EA  +++ + K RHD+ R  V QV+C
Sbjct: 55  DRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQVIC 114

Query: 76  LICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFHCE 135
           L+C TEQEV + C  C V MG+YFC +CK YDDDT KKQ+HCD CGICR+GGRENFFHC 
Sbjct: 115 LLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRIGGRENFFHCY 174

Query: 136 KCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQN 195
           KCG CYS  L++ H CVE +M   CPIC+EFLF+S  D TV+PCGHTIH +C  EM    
Sbjct: 175 KCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQKCLEEMRDHY 234

Query: 196 QYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSHTSKVRFHILGHK 255
           QY CP+CSK+V DMS  W    MEI  TPMPE Y +  V ILCNDC   ++V++H++  K
Sbjct: 235 QYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCNDCGKKAEVQYHVVAQK 294

Query: 256 CSQCNSFNTRR 265
           C  C S+NTR+
Sbjct: 295 CPNCKSYNTRQ 305

[Close]
BLAST of CmaCh17G011260 vs. TAIR 10
Match: AT5G22920.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 336.7 bits (862), Expect = 1.8e-92
Identity = 144/267 (53.93%), Postives = 185/267 (69.29%), Query Frame = 0 

Query: 4   EDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLS-NPKDRH 63
           E+    + A   + G   +GC HYRRRC+IRAPCCD IF CRHCHNEA  SL      RH
Sbjct: 7   ENEQNQEFANLMEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRH 66

Query: 64  DLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGIC 123
           +L R +V +V+C +C TEQ+V ++CS C V MG+YFC  CKF+DDD  KKQ+HCD CGIC
Sbjct: 67  ELPRHEVSKVICSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGIC 126

Query: 124 RVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTI 183
           R GG ENFFHC++C  CYS  + D H CVE +M   CP+C+E+LFDS +D TV+ CGHT+
Sbjct: 127 RTGGEENFFHCKRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTM 186

Query: 184 HLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSH 243
           HLEC ++M   N+Y CP+CSK++ DMS  W  L  E+   PMP+ Y ++ V ILCNDC  
Sbjct: 187 HLECTKDMGLHNRYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGS 246

Query: 244 TSKVRFHILGHKCSQCNSFNTRRISTG 269
            + VRFH++ HKCS C S+NTR+   G
Sbjct: 247 NTNVRFHLIAHKCSSCGSYNTRQTQRG 273

[Close]
BLAST of CmaCh17G011260 vs. TAIR 10
Match: AT5G25560.3 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 331.6 bits (849), Expect = 5.8e-91
Identity = 146/261 (55.94%), Postives = 179/261 (68.58%), Query Frame = 0 

Query: 16  DFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLS-NPKDRHDLVRQDVR---- 75
           D G  ++GC HYRRRC IRAPCC+ IF C HCH EA  +++ + K RHD+ R  V     
Sbjct: 55  DRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQLTR 114

Query: 76  ------QVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRV 135
                 QV+CL+C TEQEV + C  C V MG+YFC +CK YDDDT KKQ+HCD CGICR+
Sbjct: 115 PLSSSSQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRI 174

Query: 136 GGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHL 195
           GGRENFFHC KCG CYS  L++ H CVE +M   CPIC+EFLF+S  D TV+PCGHTIH 
Sbjct: 175 GGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQ 234

Query: 196 ECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSHTS 255
           +C  EM    QY CP+CSK+V DMS  W    MEI  TPMPE Y +  V ILCNDC   +
Sbjct: 235 KCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCNDCGKKA 294

Query: 256 KVRFHILGHKCSQCNSFNTRR 265
           +V++H++  KC  C S+NTR+
Sbjct: 295 EVQYHVVAQKCPNCKSYNTRQ 315

The following BLAST results are available for this feature:
BLAST of CmaCh17G011260 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8VZK05.3e-10563.78E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 ... [more]
Q9FFB62.5e-9153.93E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=... [more]
Q5JL965.3e-8953.58Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=3... [more]
Q10LI11.0e-8754.51E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SR... [more]
Q9CR504.1e-6544.62RING finger and CHY zinc finger domain-containing protein 1 OS=Mus musculus OX=1... [more]
BLAST of CmaCh17G011260 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JVS41.6e-168100.00E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita maxima OX=3661 GN=LOC1114887... [more]
A0A6J1GP213.8e-16798.90E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1D9475.6e-15894.80E3 ubiquitin-protein ligase MIEL1 OS=Momordica charantia OX=3673 GN=LOC111018400... [more]
A0A6J1KQY22.3e-15694.38E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita maxima OX=3661 GN=LOC111497863 PE... [more]
A0A6J1H4Y62.6e-15593.63E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita moschata OX=3662 GN=LOC111460617 ... [more]
BLAST of CmaCh17G011260 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022992470.13.2e-168100.00E3 ubiquitin-protein ligase MIEL1-like [Cucurbita maxima][more]
XP_022953225.17.9e-16798.90E3 ubiquitin-protein ligase MIEL1-like [Cucurbita moschata] >KAG6575860.1 E3 ubi... [more]
XP_023549279.11.0e-16698.90E3 ubiquitin-protein ligase MIEL1-like [Cucurbita pepo subsp. pepo][more]
XP_022150148.11.1e-15794.80E3 ubiquitin-protein ligase MIEL1 [Momordica charantia] >XP_022150149.1 E3 ubiqu... [more]
XP_023004622.14.8e-15694.38E3 ubiquitin-protein ligase MIEL1 [Cucurbita maxima] >XP_023513585.1 E3 ubiquiti... [more]
BLAST of CmaCh17G011260 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G62970.11.5e-11567.30zinc finger (C3HC4-type RING finger) family protein [more]
AT5G18650.13.7e-10663.78CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.12.1e-9358.17CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G22920.11.8e-9253.93CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.35.8e-9155.94CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 159..201
e-value: 7.1E-8
score: 42.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 159..201
e-value: 4.1E-8
score: 33.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 159..202
score: 12.282736
IPR039512RCHY1, zinc-ribbonPFAMPF14599zinc_ribbon_6coord: 206..263
e-value: 1.7E-23
score: 82.4
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 142..210
e-value: 7.8E-11
score: 43.2
IPR008913Zinc finger, CHY-typePFAMPF05495zf-CHYcoord: 24..104
e-value: 6.7E-20
score: 71.4
IPR008913Zinc finger, CHY-typePROSITEPS51266ZF_CHYcoord: 17..92
score: 24.540371
NoneNo IPR availableGENE3D2.20.28.10coord: 229..271
e-value: 1.6E-17
score: 64.4
NoneNo IPR availablePANTHERPTHR21319RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1coord: 15..265
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 158..209
IPR031109Ring finger and CHY zinc finger domain-containing protein, plantPANTHERPTHR21319:SF12ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEINcoord: 15..265
IPR017921Zinc finger, CTCHY-typePROSITEPS51270ZF_CTCHYcoord: 94..158
score: 32.596294
IPR037275Zinc finger, CTCHY-type superfamilySUPERFAMILY161245Zinc hairpin stackcoord: 95..152
IPR037274Zinc finger, CHY-type superfamilySUPERFAMILY161219CHY zinc finger-likecoord: 20..93

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G011260.1CmaCh17G011260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0016746 acyltransferase activity
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding